RchiOBHm_Chr1g0375881

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0012744
Physical Location & Seq
Forward (+)
63193201 .. 63195063
1863 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1629 bp
ATGTTTTTCTGGTTATTATTGCTATGCAATGAGTCTCACCTGCCAACTGCAATGGGCAATTACACTCCCCTTTTATACCCATCTGCACCTTTATTTACCTCTATCTCAACTTCCTTTCCACTACCAAAAAAATCTTTTCTCTTCTCTTTCTCATCTCCAATGGCACCTCTAGTTTCTAAGCAATGCCCCAACTCCACCACCCCTTCCACTTTTTTATCAAAGCAGATTCTTTCAACTCTCTCCCAAAGTCTAGGACTTTTGGTGCTCATACTTGTAACATCCCTTGAAGCCTTGTTTCTGCTCCAAAAAAGACCACCCATTTTTCACTTCATTAACCTCTCTTGCTATACCATCGTCTTCATCCTCAAGCCTTACCTCTCCAAAAAGACTCATAAAGTCTACTTGGTAGACTTCTCATGTCTAAAACCACCAAGCTCCTGCAAAGTCCCCTTCTCCAAATTCCTCAAGAACTCCTCCATGATTGAAGGTTTCGACAGTGAAAGCATATCTTTCATGGCCAACATCCTCACTTCTTCGGGCCAAAGCCAAGAGACCTATCTCCCTCCAGCTTTACACCACATTCCTCCCAAAACCCACCAACATGAATACATCAAAGAAGTTCACATGGTCCTTTTCCCCATCATGGATGACCTTTTGGCCAAAACCAAACTCTCCCCTCAAGACATTGACATCCTAATAGTAAATTGTAGCGGTTTCAATCCCTCTCCTTCTCTAGCCTCCATTGTCATTAACCACTACTCCATGAGAACTGACATCAAGAGCTTCAACCTCTCAGGAATGGGATGCAGCGCAGGCGCTCTGAGCATTGATCTGGCTCAAAATCTCCTCCAAATTCACAGGAACTCATACGCCGTTGTTCTCAGCACAGAGATTTTATCGACAGGTTGGTATGCCGGCCATGAAAAACCCAAGTTGATCCTCAACTGCCTCTTCAGAATGGGCAGCGCAGCCATTTTGCTCACAAACAAAAACCAAACATCCAAATCTCCAAAGTACGAACTGTTCAAAACACTCAGAACCCAGAAAGCCTGCGACGACAGAGCTTACCTCTCCACCATTCGAGAAGAAGACTCCGACGGAAAACTCGGCGTCACCATCAAACGTGATCTACTACAAGTCGCCGGCGAGACGCTCCGAGAAAACGTCACCGTTTTGGGGTCGTCAATCCTTCCATTATCTGAGAAATTCTGGCACGGTGTTTCGATAATCAGGAAGAGATTCTTAAACAAGTCTGAGGAGATTTACGTTCCGAATTTCAAGAGAGTGATAGAGCATTTTTGTCTGCCGGCGTCGGGGAGACCGGTGATAAGGGAAATCGGGAAAGGTTTGAAGCTTGGAGATAGGGAAATGGAGGCTGCTGTGATGACGTTGCACAGGTTTGGGAACCAGTCGTCTTCTTCGTTGTGGTATGAATTGGCGTACTTGGAGGGAAAAGAGAGGGTGAAGAAAGGTGACAAGGTTTGGCAGCTTGGGATGGGGACTGGGCCGAAGTGCACTAGTTTGGTTTGGAAGTGTATAAGGCCCATTGAGGGTGAGTCCAAGAAAGGCCCGTGGGCCGATTGCATCCATCAGTATCCATTTGAAGCCCACGGTGAAGGAGCTAATTAG

Protein Analysis

542

Amino Acids

60.66

Weight (kDa)

9.12

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 126 - 413 1.7e-110 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 430 - 511 6.7e-08 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012744)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15090 AT2G46720 AT3G10280 AT4G34250 AT4G34520
fragaria_vesca FvH4_7g28500
malus_domestica MD01G1188700.v1.1
prunus_persica Prupe.2G285400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0375881
rosa_laevigata RLG00000026647
rosa_multiflora Rmu_sc0001943.1_g000009
rosa_roxburghii Rroxscaffold_4G00282560
rosa_rugosa Rorug01G0392100
rosa_samantha Rh1AG404000 Rh1BG367400 Rh1CG380200 Rh1DG397400
rosa_wichuraiana Rw0G000970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 48
Acc36I ACCTGC 1 cut(s) 48
AccB1I GGYRCC 1 cut(s) 163
AccI GTMKAC 2 cut(s) 399, 408
AciI CCGC 1 cut(s) 711
AclWI GGATC 1 cut(s) 931
AcoI YGGCCR 3 cut(s) 516, 657, 916
AcsI RAATTY 4 cut(s) 458, 852, 1205, 1273
AcuI CTGAAG 1 cut(s) 937
AcyI GRCGYC 2 cut(s) 1110, 1310
AfaI GTAC 2 cut(s) 1016, 1442
AfiI CCNNNNNNNGG 4 cut(s) 643, 1176, 1313, 1550
AgeI ACCGGT 1 cut(s) 1321
AgsI TTSAA 9 cut(s) 234, 287, 485, 718, 787, 1027, 1279, 1351, 1604
AhlI ACTAGT 1 cut(s) 1517
AluBI AGCT 7 cut(s) 435, 569, 783, 1064, 1354, 1489, 1622
AluI AGCT 7 cut(s) 435, 569, 783, 1064, 1354, 1489, 1622
Alw21I GWGCWC 2 cut(s) 267, 1517
Alw26I GTCTC 4 cut(s) 39, 545, 1142, 1312
Alw44I GTGCAC 1 cut(s) 1513
AlwI GGATC 1 cut(s) 931
AoxI GGCC 8 cut(s) 516, 538, 657, 916, 1505, 1541, 1567, 1575
ApaLI GTGCAC 1 cut(s) 1513
ApeKI GCWGC 5 cut(s) 807, 963, 968, 1376, 1486
ApoI RAATTY 4 cut(s) 458, 852, 1205, 1273
AsiGI ACCGGT 1 cut(s) 1321
Asp700I GAANNNNTTC 1 cut(s) 1238
AspLEI GCGC 3 cut(s) 812, 818, 968
AspS9I GGNCC 6 cut(s) 538, 628, 1505, 1542, 1568, 1575
AsuHPI GGTGA 8 cut(s) 29, 1105, 1159, 1336, 1474, 1484, 1565, 1625
AvaII GGWCC 1 cut(s) 628
BaeGI GKGCMC 1 cut(s) 1517
BalI TGGCCA 2 cut(s) 518, 659
BanI GGYRCC 1 cut(s) 163
BbsI GAAGAC 3 cut(s) 349, 1095, 1407
Bbv12I GWGCWC 2 cut(s) 267, 1517
BbvI GCAGC 5 cut(s) 819, 975, 980, 1363, 1498
BccI CCATC 6 cut(s) 88, 359, 647, 1124, 1489, 1596
BceAI ACGGC 1 cut(s) 857
BciVI GTATCC 1 cut(s) 1605
BcoDI GTCTC 4 cut(s) 39, 545, 1142, 1312
BcuI ACTAGT 1 cut(s) 1517
BfaI CTAG 4 cut(s) 170, 251, 734, 1518
BfoI RGCGCY 1 cut(s) 819
BfuAI ACCTGC 1 cut(s) 48
BfuI GTATCC 1 cut(s) 1605
BisI GCNGC 5 cut(s) 808, 964, 969, 1377, 1487
BlsI GCNGC 5 cut(s) 809, 965, 970, 1378, 1488
Bme18I GGWCC 1 cut(s) 628
BmgT120I GGNCC 6 cut(s) 538, 628, 1505, 1542, 1568, 1575
BmiI GGNNCC 2 cut(s) 165, 1406
BmrI ACTGGG 1 cut(s) 1512
BmsI GCATC 2 cut(s) 794, 1593
BmuI ACTGGG 1 cut(s) 1512
BpiI GAAGAC 3 cut(s) 349, 1095, 1407
BplI GAGNNNNNCTC 2 cut(s) 1053, 1085
BpmI CTGGAG 1 cut(s) 549
BpuEI CTTGAG 3 cut(s) 350, 449, 663
BsaHI GRCGYC 2 cut(s) 1110, 1310
BsaI GGTCTC 2 cut(s) 545, 1312
BsaJI CCNNGG 2 cut(s) 1571, 1609
BsaWI WCCGGW 1 cut(s) 1321
BsaXI ACNNNNNCTCC 2 cut(s) 743, 773
Bsc4I CCNNNNNNNGG 4 cut(s) 643, 1176, 1313, 1550
Bse118I RCCGGY 4 cut(s) 914, 1142, 1306, 1321
Bse1I ACTGG 2 cut(s) 1408, 1507
Bse3DI GCAATG 3 cut(s) 34, 57, 188
BseDI CCNNGG 2 cut(s) 1571, 1609
BseGI GGATG 9 cut(s) 278, 360, 522, 652, 690, 809, 998, 1500, 1584
BseLI CCNNNNNNNGG 4 cut(s) 643, 1176, 1313, 1550
BseMI GCAATG 3 cut(s) 34, 57, 188
BseMII CTCAG 6 cut(s) 807, 812, 895, 1048, 1191, 1245
BseNI ACTGG 2 cut(s) 1408, 1507
BseRI GAGGAG 3 cut(s) 463, 836, 1271
BseSI GKGCMC 1 cut(s) 1517
BseXI GCAGC 5 cut(s) 819, 975, 980, 1363, 1498
BsgI GTGCAG 1 cut(s) 69
BshFI GGCC 8 cut(s) 518, 540, 659, 918, 1507, 1543, 1569, 1577
BshNI GGYRCC 1 cut(s) 163
BshTI ACCGGT 1 cut(s) 1321
BsiHKAI GWGCWC 2 cut(s) 267, 1517
BsiSI CCGG 4 cut(s) 915, 1143, 1307, 1322
BslFI GGGAC 2 cut(s) 431, 1513
BslI CCNNNNNNNGG 4 cut(s) 643, 1176, 1313, 1550
BsmAI GTCTC 4 cut(s) 39, 545, 1142, 1312
BsmBI CGTCTC 1 cut(s) 1142
BsmFI GGGAC 2 cut(s) 431, 1513
BsnI GGCC 8 cut(s) 518, 540, 659, 918, 1507, 1543, 1569, 1577
Bso31I GGTCTC 2 cut(s) 545, 1312
Bsp1286I GDGCHC 2 cut(s) 267, 1517
Bsp143I GATC 3 cut(s) 829, 936, 1126
BspACI CCGC 1 cut(s) 711
BspANI GGCC 8 cut(s) 518, 540, 659, 918, 1507, 1543, 1569, 1577
BspCNI CTCAG 6 cut(s) 806, 813, 894, 1047, 1192, 1246
BspLI GGNNCC 2 cut(s) 165, 1406
BspMI ACCTGC 1 cut(s) 48
BspPI GGATC 1 cut(s) 931
BspT107I GGYRCC 1 cut(s) 163
BspTNI GGTCTC 2 cut(s) 545, 1312
BsrDI GCAATG 3 cut(s) 34, 57, 188
BsrFI RCCGGY 4 cut(s) 914, 1142, 1306, 1321
BsrI ACTGG 2 cut(s) 1408, 1507
BssAI RCCGGY 4 cut(s) 914, 1142, 1306, 1321
BssECI CCNNGG 2 cut(s) 1571, 1609
BssMI GATC 3 cut(s) 829, 936, 1126
BssNI GRCGYC 2 cut(s) 1110, 1310
Bst4CI ACNGT 5 cut(s) 497, 1023, 1171, 1217, 1613
Bst6I CTCTTC 3 cut(s) 146, 956, 1229
BstACI GRCGYC 2 cut(s) 1110, 1310
BstC8I GCNNGC 5 cut(s) 814, 916, 1051, 1144, 1308
BstDEI CTNAG 7 cut(s) 177, 793, 821, 881, 1034, 1200, 1254
BstDSI CCRYGG 2 cut(s) 1571, 1609
BstF5I GGATG 9 cut(s) 278, 360, 522, 652, 690, 809, 998, 1500, 1584
BstH2I RGCGCY 1 cut(s) 819
BstHHI GCGC 3 cut(s) 812, 818, 968
BstKTI GATC 3 cut(s) 832, 939, 1129
BstMAI GTCTC 4 cut(s) 39, 545, 1142, 1312
BstMBI GATC 3 cut(s) 829, 936, 1126
BstMWI GCNNNNNNNGC 2 cut(s) 813, 822
BstSLI GKGCMC 1 cut(s) 1517
BstV1I GCAGC 5 cut(s) 819, 975, 980, 1363, 1498
BstV2I GAAGAC 3 cut(s) 349, 1095, 1407
BsuI GTATCC 1 cut(s) 1605
BsuRI GGCC 8 cut(s) 518, 540, 659, 918, 1507, 1543, 1569, 1577
BtgI CCRYGG 2 cut(s) 1571, 1609
BtsCI GGATG 9 cut(s) 278, 360, 522, 652, 690, 809, 998, 1500, 1584
BtsIMutI CAGTG 1 cut(s) 502
BveI ACCTGC 1 cut(s) 48
Cac8I GCNNGC 5 cut(s) 814, 916, 1051, 1144, 1308
CfoI GCGC 3 cut(s) 812, 818, 968
Cfr10I RCCGGY 4 cut(s) 914, 1142, 1306, 1321
Cfr13I GGNCC 6 cut(s) 538, 628, 1505, 1542, 1568, 1575
CseI GACGC 3 cut(s) 1099, 1159, 1299
Csp6I GTAC 2 cut(s) 1015, 1441
CspAI ACCGGT 1 cut(s) 1321
CviAII CATG 8 cut(s) 417, 478, 514, 602, 625, 643, 763, 920
CviQI GTAC 2 cut(s) 1015, 1441
DdeI CTNAG 7 cut(s) 177, 793, 821, 881, 1034, 1200, 1254
DpnI GATC 3 cut(s) 831, 938, 1128
DpnII GATC 3 cut(s) 829, 936, 1126
EaeI YGGCCR 3 cut(s) 516, 657, 916
Eam1104I CTCTTC 3 cut(s) 146, 956, 1229
EarI CTCTTC 3 cut(s) 146, 956, 1229
Eco31I GGTCTC 2 cut(s) 545, 1312
Eco47I GGWCC 1 cut(s) 628
Eco57I CTGAAG 1 cut(s) 937
Esp3I CGTCTC 1 cut(s) 1142
FaeI CATG 8 cut(s) 420, 481, 517, 605, 628, 646, 766, 923
FalI AAGNNNNNCTT 4 cut(s) 493, 525, 1226, 1258
FaqI GGGAC 2 cut(s) 431, 1513
FatI CATG 8 cut(s) 416, 477, 513, 601, 624, 642, 762, 919
FblI GTMKAC 2 cut(s) 399, 408
Fnu4HI GCNGC 5 cut(s) 808, 964, 969, 1377, 1487
FokI GGATG 9 cut(s) 265, 347, 509, 659, 677, 816, 985, 1507, 1571
Fsp4HI GCNGC 5 cut(s) 808, 964, 969, 1377, 1487
FspBI CTAG 4 cut(s) 170, 251, 734, 1518
GlaI GCGC 3 cut(s) 811, 817, 967
GluI GCNGC 5 cut(s) 808, 964, 969, 1377, 1487
GsuI CTGGAG 1 cut(s) 549
HaeII RGCGCY 1 cut(s) 819
HaeIII GGCC 8 cut(s) 518, 540, 659, 918, 1507, 1543, 1569, 1577
HapII CCGG 4 cut(s) 915, 1143, 1307, 1322
HgaI GACGC 3 cut(s) 1099, 1159, 1299
HhaI GCGC 3 cut(s) 812, 818, 968
Hin1I GRCGYC 2 cut(s) 1110, 1310
Hin1II CATG 8 cut(s) 420, 481, 517, 605, 628, 646, 766, 923
Hin6I GCGC 3 cut(s) 810, 816, 966
HinP1I GCGC 3 cut(s) 810, 816, 966
HindIII AAGCTT 1 cut(s) 1352
HinfI GANTC 6 cut(s) 32, 226, 388, 1091, 1239, 1556
HpaII CCGG 4 cut(s) 915, 1143, 1307, 1322
HphI GGTGA 8 cut(s) 29, 1105, 1159, 1336, 1474, 1484, 1565, 1625
Hpy166II GTNNAC 4 cut(s) 400, 409, 622, 1515
Hpy188I TCNGA 8 cut(s) 822, 956, 1037, 1096, 1157, 1201, 1255, 1272
Hpy188III TCNNGA 8 cut(s) 466, 680, 778, 795, 1082, 1231, 1279, 1339
Hpy8I GTNNAC 4 cut(s) 400, 409, 622, 1515
Hpy99I CGWCG 3 cut(s) 1058, 1100, 1315
HpyAV CCTTC 6 cut(s) 213, 460, 479, 738, 1199, 1610
HpyCH4III ACNGT 5 cut(s) 497, 1023, 1171, 1217, 1613
HpyCH4IV ACGT 4 cut(s) 1123, 1164, 1266, 1388
HpyCH4V TGCA 8 cut(s) 27, 50, 86, 441, 807, 1393, 1515, 1584
HpyF10VI GCNNNNNNNGC 2 cut(s) 813, 822
HpyF3I CTNAG 7 cut(s) 177, 793, 821, 881, 1034, 1200, 1254
HpySE526I ACGT 4 cut(s) 1123, 1164, 1266, 1388
Hsp92I GRCGYC 2 cut(s) 1110, 1310
Hsp92II CATG 8 cut(s) 420, 481, 517, 605, 628, 646, 766, 923
HspAI GCGC 3 cut(s) 810, 816, 966
KroI GCCGGC 3 cut(s) 914, 1142, 1306
KroNI GCCGGC 3 cut(s) 916, 1144, 1308
Kzo9I GATC 3 cut(s) 829, 936, 1126
LmnI GCTCC 4 cut(s) 306, 440, 1158, 1619
Lsp1109I GCAGC 5 cut(s) 819, 975, 980, 1363, 1498
LweI GCATC 2 cut(s) 794, 1593
MaeI CTAG 4 cut(s) 170, 251, 734, 1518
MaeII ACGT 4 cut(s) 1123, 1164, 1266, 1388
MaeIII GTNAC 4 cut(s) 274, 1111, 1165, 1472
MalI GATC 3 cut(s) 831, 938, 1128
MboI GATC 3 cut(s) 829, 936, 1126
MhlI GDGCHC 2 cut(s) 267, 1517
MlsI TGGCCA 2 cut(s) 518, 659
MluCI AATT 8 cut(s) 58, 458, 703, 852, 1205, 1273, 1433, 1624
MluNI TGGCCA 2 cut(s) 518, 659
MlyI GAGTC 4 cut(s) 41, 382, 1085, 1565
MmeI TCCRAC 1 cut(s) 1119
Mox20I TGGCCA 2 cut(s) 518, 659
MreI CGCCGGCG 1 cut(s) 1142
MroNI GCCGGC 3 cut(s) 914, 1142, 1306
MroXI GAANNNNTTC 1 cut(s) 1238
MscI TGGCCA 2 cut(s) 518, 659
MseI TTAA 3 cut(s) 333, 750, 1244
MslI CAYNNNNRTG 1 cut(s) 600
Msp20I TGGCCA 2 cut(s) 518, 659
MspI CCGG 4 cut(s) 915, 1143, 1307, 1322
MwoI GCNNNNNNNGC 2 cut(s) 813, 822
NaeI GCCGGC 3 cut(s) 916, 1144, 1308
NdeII GATC 3 cut(s) 829, 936, 1126
NgoMIV GCCGGC 3 cut(s) 914, 1142, 1306
NlaIII CATG 8 cut(s) 420, 481, 517, 605, 628, 646, 766, 923
NlaIV GGNNCC 2 cut(s) 165, 1406
NmeAIII GCCGAG 1 cut(s) 1086
NmuCI GTSAC 3 cut(s) 1111, 1165, 1472
PaqCI CACCTGC 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 1418
PdiI GCCGGC 3 cut(s) 916, 1144, 1308
PdmI GAANNNNTTC 1 cut(s) 1238
PfeI GAWTC 2 cut(s) 226, 1239
PinAI ACCGGT 1 cut(s) 1321
PkrI GCNGC 5 cut(s) 809, 965, 970, 1378, 1488
PleI GAGTC 4 cut(s) 40, 382, 1085, 1564
PpsI GAGTC 4 cut(s) 40, 382, 1085, 1564
PspN4I GGNNCC 2 cut(s) 165, 1406
PspPI GGNCC 6 cut(s) 538, 628, 1505, 1542, 1568, 1575
PsrI GAACNNNNNNTAC 2 cut(s) 1007, 1039
RsaI GTAC 2 cut(s) 1016, 1442
RsaNI GTAC 2 cut(s) 1015, 1441
RseI CAYNNNNRTG 1 cut(s) 600
SaqAI TTAA 3 cut(s) 333, 750, 1244
SatI GCNGC 5 cut(s) 808, 964, 969, 1377, 1487
Sau3AI GATC 3 cut(s) 829, 936, 1126
Sau96I GGNCC 6 cut(s) 538, 628, 1505, 1542, 1568, 1575
SchI GAGTC 4 cut(s) 41, 382, 1085, 1565
SduI GDGCHC 2 cut(s) 267, 1517
SfaNI GCATC 2 cut(s) 794, 1593
SgrAI CRCCGGYG 1 cut(s) 1142
SinI GGWCC 1 cut(s) 628
SmiMI CAYNNNNRTG 1 cut(s) 600
SmlI CTYRAG 3 cut(s) 365, 464, 678
SmoI CTYRAG 3 cut(s) 365, 464, 678
SpeI ACTAGT 1 cut(s) 1517
Sse9I AATT 8 cut(s) 58, 458, 703, 852, 1205, 1273, 1433, 1624
SsiI CCGC 1 cut(s) 711
SspMI CTAG 4 cut(s) 170, 251, 734, 1518
TaaI ACNGT 5 cut(s) 497, 1023, 1171, 1217, 1613
TaiI ACGT 4 cut(s) 1126, 1167, 1269, 1391
TaqI TCGA 4 cut(s) 492, 899, 1081, 1223
TasI AATT 8 cut(s) 58, 458, 703, 852, 1205, 1273, 1433, 1624
TfiI GAWTC 2 cut(s) 226, 1239
Tru1I TTAA 3 cut(s) 333, 750, 1244
Tru9I TTAA 3 cut(s) 333, 750, 1244
TscAI CASTG 1 cut(s) 502
TseFI GTSAC 3 cut(s) 1111, 1165, 1472
TseI GCWGC 5 cut(s) 807, 963, 968, 1376, 1486
Tsp45I GTSAC 3 cut(s) 1111, 1165, 1472
TspDTI ATGAA 6 cut(s) 319, 349, 502, 618, 936, 1446
TspGWI ACGGA 1 cut(s) 1113
TspRI CASTG 1 cut(s) 502
VneI GTGCAC 1 cut(s) 1513
VpaK11BI GGWCC 1 cut(s) 628
XapI RAATTY 4 cut(s) 458, 852, 1205, 1273
XmiI GTMKAC 2 cut(s) 399, 408
XmnI GAANNNNTTC 1 cut(s) 1238
XspI CTAG 4 cut(s) 170, 251, 734, 1518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.