RchiOBHm_Chr1g0377771

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0015281
Physical Location & Seq
Forward (+)
64513441 .. 64515574
2134 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 987 bp
ATGAAAATCTTCCTGAAATTTTCTTCTTCTTCTTCTTGTTCTGAATCTTCAATGGCTTCTTCGCTTCTCTCAATCTTTCTTCTTTTGCCGATCTTCTCCATCTCCTCCTGCTCTGCCGAAACTAGCAGGAAGGAGTTAAGGAGTAAGGAATTCAACCAGGAAGCATTGATACAGTTGGGTCACTCGGTTGATTACAACAGAATTGACCCGTCAAGGGTTGTTCATCTTTCTTGGCGACCAAGGGTGTTTCTGTACAAAGGATTCCTATCGGATGAGGAGTGTGACCACCTTATTTCTTTGGCAAATGGTGGAGAAGAGAAGTCTTCGACAGAATATGATGAAGCTGGAAACAGCAACACAAATAGGATGCTTAAAAGTTTAGACCTTCCCTTGAGCATGGGGGATGGTATAGTTTCAAAGATTGAGGAAAAGATCTCGGCCTGGACTTTCCTTCCTAAAGAGAACAGCAGAGCTTTACAGGTTTTGCATTACGACCTTGAGGAGGCAGAGAAGATTTACAATTATTTTGGCAACAAATCCACATTGGAACAAAGTGAGCCCTTGTTAGCAACAGTTATTCTTTATCTCTCAAACGTCACTCGTGGTGGTGAGATTCTACTCCCGGAGTCAGAGCTGAAGAGCAATGCCCAGTCTGGTTGTGGAAAGAGTAGCAGTATCTTGAAACCAACCAAAGGAAACGCAATTCTGTTTTTCAATCTCCATCCTAATGGATCTCCCGACAAGAGCAGTTCCCATGCCCGATGCCCCATACTCGAAGGTGAAATGTGGTGCGCCACAAAGTTCCTTCATGTGAAAGCCATTCCTCAGGAAAATTCCTCCTCCAATTCTGATAGCAGCGATTGCACTGATGAAGACGATAGCTGTCCCAGGTGGGCAGACATTGGGGAGTGTCAAAGGAACCCTGTATTCATGATTGGCTCCGATGATTACTACGGTACATGCAGGAAGAGTTGTAATGCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 map00330 map01100
Pfam Domains
Protein Families
2OG-FeII_Oxy_3 ShK

Protein Analysis

328

Amino Acids

36.38

Weight (kDa)

5.22

Isoelectric Point (pI)

55.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25600 AT4G25600 AT4G25600 AT4G25600
fragaria_vesca FvH4_7g29771 FvH4_7g29771
malus_domestica MD07G1273300.v1.1
prunus_persica Prupe.2G297000_v2.0.a1
pyrus_communis pycom07g24990
rosa_chinensis RchiOBHm_Chr1g0377771
rosa_laevigata RLG00000026500
rosa_multiflora Rmu_sc0005758.1_g000003
rosa_roxburghii Rroxscaffold_4G00281070
rosa_rugosa Rorug01G0404900
rosa_samantha Rh1AG427400
rosa_wichuraiana Rw1G037110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 739
AcsI RAATTY 3 cut(s) 17, 149, 832
AcuI CTGAAG 1 cut(s) 656
AfaI GTAC 2 cut(s) 254, 958
AfiI CCNNNNNNNGG 3 cut(s) 214, 502, 692
AgsI TTSAA 5 cut(s) 51, 154, 417, 682, 715
AjnI CCWGG 3 cut(s) 156, 440, 887
AluBI AGCT 4 cut(s) 344, 473, 634, 882
AluI AGCT 4 cut(s) 344, 473, 634, 882
AlwI GGATC 1 cut(s) 739
AoxI GGCC 1 cut(s) 438
ApeKI GCWGC 1 cut(s) 855
ApoI RAATTY 3 cut(s) 17, 149, 832
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 794
AsuC2I CCSGG 1 cut(s) 623
AsuHPI GGTGA 2 cut(s) 620, 791
AxyI CCTNAGG 1 cut(s) 825
BanII GRGCYC 1 cut(s) 561
BarI GAAGNNNNNNTAC 2 cut(s) 153, 185
BauI CACGAG 1 cut(s) 600
BbsI GAAGAC 2 cut(s) 315, 879
BbvI GCAGC 1 cut(s) 867
BccI CCATC 3 cut(s) 107, 398, 729
BciT130I CCWGG 3 cut(s) 158, 442, 889
BcnI CCSGG 1 cut(s) 623
BfaI CTAG 1 cut(s) 123
BglII AGATCT 1 cut(s) 432
BisI GCNGC 1 cut(s) 856
BlsI GCNGC 1 cut(s) 857
Bme1390I CCNGG 4 cut(s) 158, 442, 623, 889
BmiI GGNNCC 2 cut(s) 920, 940
BmrFI CCNGG 4 cut(s) 158, 442, 623, 889
BmrI ACTGGG 1 cut(s) 643
BmsI GCATC 2 cut(s) 357, 752
BmuI ACTGGG 1 cut(s) 643
BpiI GAAGAC 2 cut(s) 315, 879
BpuEI CTTGAG 2 cut(s) 412, 518
BpuMI CCSGG 1 cut(s) 623
BsaBI GATNNNNATC 1 cut(s) 265
BsaJI CCNNGG 2 cut(s) 239, 887
Bsc4I CCNNNNNNNGG 3 cut(s) 214, 502, 692
Bse1I ACTGG 1 cut(s) 649
Bse21I CCTNAGG 1 cut(s) 825
Bse3DI GCAATG 1 cut(s) 649
Bse8I GATNNNNATC 1 cut(s) 265
BseBI CCWGG 3 cut(s) 158, 442, 889
BseDI CCNNGG 2 cut(s) 239, 887
BseGI GGATG 4 cut(s) 277, 372, 409, 721
BseJI GATNNNNATC 1 cut(s) 265
BseLI CCNNNNNNNGG 3 cut(s) 214, 502, 692
BseMI GCAATG 1 cut(s) 649
BseMII CTCAG 1 cut(s) 839
BseNI ACTGG 1 cut(s) 649
BseRI GAGGAG 4 cut(s) 94, 290, 515, 829
BseXI GCAGC 1 cut(s) 867
BshFI GGCC 1 cut(s) 440
BsiSI CCGG 1 cut(s) 623
BslFI GGGAC 1 cut(s) 870
BslI CCNNNNNNNGG 3 cut(s) 214, 502, 692
BsmFI GGGAC 1 cut(s) 870
BsnI GGCC 1 cut(s) 440
Bsp1286I GDGCHC 1 cut(s) 561
Bsp1407I TGTACA 1 cut(s) 252
Bsp143I GATC 3 cut(s) 90, 432, 731
BspANI GGCC 1 cut(s) 440
BspCNI CTCAG 1 cut(s) 838
BspHI TCATGA 1 cut(s) 930
BspLI GGNNCC 2 cut(s) 920, 940
BspPI GGATC 1 cut(s) 739
BspQI GCTCTTC 1 cut(s) 632
BsrDI GCAATG 1 cut(s) 649
BsrGI TGTACA 1 cut(s) 252
BsrI ACTGG 1 cut(s) 649
BssECI CCNNGG 2 cut(s) 239, 887
BssMI GATC 3 cut(s) 90, 432, 731
BssSI CACGAG 1 cut(s) 600
BssT1I CCWWGG 1 cut(s) 239
Bst2BI CACGAG 1 cut(s) 600
Bst2UI CCWGG 3 cut(s) 158, 442, 889
Bst4CI ACNGT 3 cut(s) 174, 574, 956
Bst6I CTCTTC 3 cut(s) 309, 632, 962
BstAPI GCANNNNNTGC 1 cut(s) 861
BstAUI TGTACA 1 cut(s) 252
BstC8I GCNNGC 1 cut(s) 982
BstDEI CTNAG 1 cut(s) 825
BstENI CCTNNNNNAGG 1 cut(s) 500
BstF5I GGATG 4 cut(s) 277, 372, 409, 721
BstHHI GCGC 1 cut(s) 794
BstKTI GATC 3 cut(s) 93, 435, 734
BstMBI GATC 3 cut(s) 90, 432, 731
BstMWI GCNNNNNNNGC 1 cut(s) 861
BstNI CCWGG 3 cut(s) 158, 442, 889
BstNSI RCATGY 2 cut(s) 963, 984
BstSCI CCNGG 4 cut(s) 156, 440, 621, 887
BstV1I GCAGC 1 cut(s) 867
BstV2I GAAGAC 2 cut(s) 315, 879
BstX2I RGATCY 2 cut(s) 432, 731
BstXI CCANNNNNNTGG 1 cut(s) 728
BstYI RGATCY 2 cut(s) 432, 731
Bsu36I CCTNAGG 1 cut(s) 825
BsuRI GGCC 1 cut(s) 440
BtsCI GGATG 4 cut(s) 277, 372, 409, 721
BtsIMutI CAGTG 1 cut(s) 864
Cac8I GCNNGC 1 cut(s) 982
CciI TCATGA 1 cut(s) 930
CfoI GCGC 1 cut(s) 794
Csp6I GTAC 2 cut(s) 253, 957
CviAII CATG 6 cut(s) 397, 755, 809, 931, 960, 981
CviJI RGCY 9 cut(s) 56, 344, 440, 473, 559, 634, 818, 882, 939
CviKI_1 RGCY 9 cut(s) 56, 344, 440, 473, 559, 634, 818, 882, 939
CviQI GTAC 2 cut(s) 253, 957
DdeI CTNAG 1 cut(s) 825
DpnI GATC 3 cut(s) 92, 434, 733
DpnII GATC 3 cut(s) 90, 432, 731
Eam1104I CTCTTC 3 cut(s) 309, 632, 962
EarI CTCTTC 3 cut(s) 309, 632, 962
Eco130I CCWWGG 1 cut(s) 239
Eco24I GRGCYC 1 cut(s) 561
Eco57I CTGAAG 1 cut(s) 656
Eco81I CCTNAGG 1 cut(s) 825
EcoNI CCTNNNNNAGG 1 cut(s) 500
EcoRI GAATTC 1 cut(s) 149
EcoRII CCWGG 3 cut(s) 156, 440, 887
EcoT14I CCWWGG 1 cut(s) 239
EcoT22I ATGCAT 1 cut(s) 982
EcoT38I GRGCYC 1 cut(s) 561
ErhI CCWWGG 1 cut(s) 239
FaeI CATG 6 cut(s) 400, 758, 812, 934, 963, 984
FaiI YATR 9 cut(s) 336, 398, 410, 756, 770, 810, 932, 961, 982
FaqI GGGAC 1 cut(s) 870
FatI CATG 6 cut(s) 396, 754, 808, 930, 959, 980
Fnu4HI GCNGC 1 cut(s) 856
FokI GGATG 4 cut(s) 284, 379, 416, 708
FriOI GRGCYC 1 cut(s) 561
Fsp4HI GCNGC 1 cut(s) 856
FspBI CTAG 1 cut(s) 123
GlaI GCGC 1 cut(s) 793
GluI GCNGC 1 cut(s) 856
HaeIII GGCC 1 cut(s) 440
HapII CCGG 1 cut(s) 623
HhaI GCGC 1 cut(s) 794
Hin1II CATG 6 cut(s) 400, 758, 812, 934, 963, 984
Hin6I GCGC 1 cut(s) 792
HinP1I GCGC 1 cut(s) 792
HinfI GANTC 4 cut(s) 44, 261, 613, 626
HpaII CCGG 1 cut(s) 623
HphI GGTGA 2 cut(s) 620, 791
Hpy188I TCNGA 5 cut(s) 43, 271, 631, 850, 943
Hpy188III TCNNGA 5 cut(s) 13, 679, 737, 827, 931
HpyAV CCTTC 5 cut(s) 124, 395, 461, 770, 815
HpyCH4III ACNGT 3 cut(s) 174, 574, 956
HpyCH4IV ACGT 1 cut(s) 594
HpyCH4V TGCA 4 cut(s) 487, 864, 963, 980
HpyF10VI GCNNNNNNNGC 1 cut(s) 861
HpyF3I CTNAG 1 cut(s) 825
HpySE526I ACGT 1 cut(s) 594
Hsp92II CATG 6 cut(s) 400, 758, 812, 934, 963, 984
HspAI GCGC 1 cut(s) 792
Kzo9I GATC 3 cut(s) 90, 432, 731
LguI GCTCTTC 1 cut(s) 632
LmnI GCTCC 1 cut(s) 944
Lsp1109I GCAGC 1 cut(s) 867
LweI GCATC 2 cut(s) 357, 752
MaeI CTAG 1 cut(s) 123
MaeII ACGT 1 cut(s) 594
MaeIII GTNAC 3 cut(s) 179, 281, 595
MalI GATC 3 cut(s) 92, 434, 733
MboI GATC 3 cut(s) 90, 432, 731
MflI RGATCY 2 cut(s) 432, 731
MhlI GDGCHC 1 cut(s) 561
MluCI AATT 7 cut(s) 17, 149, 201, 520, 702, 832, 844
MlyI GAGTC 1 cut(s) 635
MnlI CCTC 8 cut(s) 115, 268, 418, 493, 496, 834, 847, 850
Mph1103I ATGCAT 1 cut(s) 982
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 2 cut(s) 137, 372
MslI CAYNNNNRTG 1 cut(s) 726
MspI CCGG 1 cut(s) 623
MspR9I CCNGG 4 cut(s) 158, 442, 623, 889
MvaI CCWGG 3 cut(s) 158, 442, 889
MwoI GCNNNNNNNGC 1 cut(s) 861
NciI CCSGG 1 cut(s) 623
NdeII GATC 3 cut(s) 90, 432, 731
NlaIII CATG 6 cut(s) 400, 758, 812, 934, 963, 984
NlaIV GGNNCC 2 cut(s) 920, 940
NmeAIII GCCGAG 1 cut(s) 416
NmuCI GTSAC 3 cut(s) 179, 281, 595
NsiI ATGCAT 1 cut(s) 982
NspI RCATGY 2 cut(s) 963, 984
PaeI GCATGC 1 cut(s) 984
PagI TCATGA 1 cut(s) 930
PciSI GCTCTTC 1 cut(s) 632
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 3 cut(s) 44, 261, 613
PfoI TCCNGGA 1 cut(s) 621
PkrI GCNGC 1 cut(s) 857
PleI GAGTC 1 cut(s) 634
PpsI GAGTC 1 cut(s) 634
Psp6I CCWGG 3 cut(s) 156, 440, 887
PspGI CCWGG 3 cut(s) 156, 440, 887
PspN4I GGNNCC 2 cut(s) 920, 940
PsuI RGATCY 2 cut(s) 432, 731
RsaI GTAC 2 cut(s) 254, 958
RsaNI GTAC 2 cut(s) 253, 957
RseI CAYNNNNRTG 1 cut(s) 726
SapI GCTCTTC 1 cut(s) 632
SaqAI TTAA 2 cut(s) 137, 372
SatI GCNGC 1 cut(s) 856
Sau3AI GATC 3 cut(s) 90, 432, 731
SchI GAGTC 1 cut(s) 635
ScrFI CCNGG 4 cut(s) 158, 442, 623, 889
SduI GDGCHC 1 cut(s) 561
SfaNI GCATC 2 cut(s) 357, 752
SmiMI CAYNNNNRTG 1 cut(s) 726
SmlI CTYRAG 2 cut(s) 391, 497
SmoI CTYRAG 2 cut(s) 391, 497
SphI GCATGC 1 cut(s) 984
Sse9I AATT 7 cut(s) 17, 149, 201, 520, 702, 832, 844
SspMI CTAG 1 cut(s) 123
StyD4I CCNGG 4 cut(s) 156, 440, 621, 887
StyI CCWWGG 1 cut(s) 239
TaaI ACNGT 3 cut(s) 174, 574, 956
TaiI ACGT 1 cut(s) 597
TaqI TCGA 2 cut(s) 326, 774
TasI AATT 7 cut(s) 17, 149, 201, 520, 702, 832, 844
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 3 cut(s) 44, 261, 613
Tru1I TTAA 2 cut(s) 137, 372
Tru9I TTAA 2 cut(s) 137, 372
TscAI CASTG 1 cut(s) 871
TseFI GTSAC 3 cut(s) 179, 281, 595
TseI GCWGC 1 cut(s) 855
Tsp45I GTSAC 3 cut(s) 179, 281, 595
TspDTI ATGAA 6 cut(s) 17, 212, 354, 797, 885, 919
TspRI CASTG 1 cut(s) 871
XagI CCTNNNNNAGG 1 cut(s) 500
XapI RAATTY 3 cut(s) 17, 149, 832
XceI RCATGY 2 cut(s) 963, 984
XcmI CCANNNNNNNNNTGG 1 cut(s) 656
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 123
Zsp2I ATGCAT 1 cut(s) 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.