RchiOBHm_Chr1g0380201

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0008155
Physical Location & Seq
Reverse (-)
65933720 .. 65939990
6271 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1140 bp
ATGACGAACTTTGCTAAGCTGAACCAGGCAACGGGGTTTCAGAGATCATCAACATATGATAAGCAAAAGGTGCAACCTATCAGTACAAAAGTAGTAAAGAACGACGAGAACAGTGGTGATCTTTCCTTGGCATCAATAGCCTCAAACCTGCATGATATTTCTACCATTGTTAATACACCTATCCCCTCATCAGATATAGCTTATAGTTCATGGGTGGTGGAGCACCCTTCTGCACTAGGCTCATTTGACAGGATGATGAAAGCTGCAAAAGGGAAGAGGATTGTTGTCTTTTTAGACTATGACGGCACCCTTTCACCGATTGTTGATGATCCGGATCGTGCTTTCATGTCCAATGAGATGCGTGCAGCAGTACGCGAAGTTGCCAAATATTTTCCAACAGCAGTAATCAGTGGGAGGAGTAGAGACAAGGTAAAAGGATTTGTACAGTTAAGTAATGTATATTATGCCGGCAGCCATGGGATGGACATTATGGTCCCTCCAAAGCCACTAAAGCCCTGTGATGCCACGAACCACGTCACAGCCACAGCCTCAGCCTTGGATAAGGAGGGTGACATGCTCTTTCAACCTGCCAAAAGATTCCTGCCTGCAATCCAAGAGATAAGCATAGCGTTGGAGGAAATAGCAAGAAAGATAGAAGGTGCAAGAGTAGAGGACAATAGATTCTGCATCTCTGTGCATTACCGGAAGGTCCGGGAAGAGGATTATGGCATATTAGAAGAAAAGGTGAAGTCTGTGATTGAAAACTATCCTGATTTTCACCTAACTCGGGGTAAAAAGGTCCTGGAGATACGTCCATCTATAGAATGGAACAAAGGTCATGCGCTTGAATATTTACTTGACACTCTTGGATTCAGTAATTCGGGTGATGTATTCCCATTGTACATCGGGGATGATCGAACGGATGAAGATGCTTTCAAGGTGATTGAAGCCAGAGGACAGGGATTTCCAATAATCGTGTCTTCCATCCCAAAGGACACCAAAGCTGCTTTTTCTCTGCACGATCCATCAGAAGTGTTGACTTTCTTGTTACGCCTTGCAAGGTGGAGAAAAACCTCGTCTTCGAGCAGGTCACTCGCTCAAATTTGGGGTCCTGGGAATTCACATAAGGCCAATTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Trehalose_PPase

Protein Analysis

379

Amino Acids

42.19

Weight (kDa)

7.71

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 97 - 343 4.6e-62 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 491
Acc36I ACCTGC 3 cut(s) 156, 595, 1077
AccB1I GGYRCC 1 cut(s) 305
AccB7I CCANNNNNTGG 1 cut(s) 481
AccII CGCG 1 cut(s) 375
AccIII TCCGGA 1 cut(s) 331
AclWI GGATC 3 cut(s) 323, 342, 1016
AcsI RAATTY 2 cut(s) 1101, 1117
AfaI GTAC 4 cut(s) 85, 372, 444, 902
AfiI CCNNNNNNNGG 4 cut(s) 31, 481, 718, 787
AgsI TTSAA 5 cut(s) 584, 761, 848, 937, 947
AjiI CACGTC 1 cut(s) 535
AjnI CCWGG 3 cut(s) 24, 801, 1111
AluBI AGCT 4 cut(s) 19, 200, 263, 1004
AluI AGCT 4 cut(s) 19, 200, 263, 1004
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 1 cut(s) 417
AlwI GGATC 3 cut(s) 323, 342, 1016
Ama87I CYCGRG 1 cut(s) 786
Aor13HI TCCGGA 1 cut(s) 331
AoxI GGCC 1 cut(s) 1128
ApeKI GCWGC 4 cut(s) 263, 365, 471, 1004
ApoI RAATTY 2 cut(s) 1101, 1117
ArsI GACNNNNNNTTYG 2 cut(s) 1093, 1125
AspLEI GCGC 1 cut(s) 844
AspS9I GGNCC 4 cut(s) 493, 709, 799, 1109
AsuC2I CCSGG 1 cut(s) 713
AsuHPI GGTGA 7 cut(s) 128, 306, 581, 757, 770, 896, 952
AvaI CYCGRG 1 cut(s) 786
AvaII GGWCC 4 cut(s) 493, 709, 799, 1109
BanI GGYRCC 1 cut(s) 305
BbsI GAAGAC 2 cut(s) 972, 1071
Bbv12I GWGCWC 1 cut(s) 225
BbvCI CCTCAGC 1 cut(s) 550
BbvI GCAGC 4 cut(s) 250, 377, 483, 991
BccI CCATC 4 cut(s) 475, 823, 992, 1033
BceAI ACGGC 1 cut(s) 319
BcgI CGANNNNNNTGC 2 cut(s) 1075, 1109
BciT130I CCWGG 3 cut(s) 26, 803, 1113
BcnI CCSGG 1 cut(s) 713
BcoDI GTCTC 1 cut(s) 417
BfaI CTAG 2 cut(s) 236, 1138
BfmI CTRYAG 1 cut(s) 819
BfuAI ACCTGC 3 cut(s) 156, 595, 1077
BisI GCNGC 4 cut(s) 264, 366, 472, 1005
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 4 cut(s) 265, 367, 473, 1006
Bme1390I CCNGG 4 cut(s) 26, 713, 803, 1113
Bme18I GGWCC 4 cut(s) 493, 709, 799, 1109
BmeT110I CYCGRG 1 cut(s) 786
BmgBI CACGTC 1 cut(s) 535
BmgT120I GGNCC 4 cut(s) 493, 709, 799, 1109
BmiI GGNNCC 3 cut(s) 307, 495, 1110
BmrFI CCNGG 4 cut(s) 26, 713, 803, 1113
BmsI GCATC 5 cut(s) 140, 348, 511, 696, 919
BpiI GAAGAC 2 cut(s) 972, 1071
BpmI CTGGAG 1 cut(s) 824
Bpu10I CCTNAGC 1 cut(s) 550
Bpu1102I GCTNAGC 1 cut(s) 15
BpuMI CCSGG 1 cut(s) 713
BsaBI GATNNNNATC 1 cut(s) 333
BsaJI CCNNGG 4 cut(s) 126, 475, 555, 1112
BsaWI WCCGGW 2 cut(s) 331, 702
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 481, 718, 787
Bse118I RCCGGY 1 cut(s) 467
Bse8I GATNNNNATC 1 cut(s) 333
BseAI TCCGGA 1 cut(s) 331
BseBI CCWGG 3 cut(s) 26, 803, 1113
BseDI CCNNGG 4 cut(s) 126, 475, 555, 1112
BseGI GGATG 5 cut(s) 258, 486, 916, 928, 984
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 4 cut(s) 31, 481, 718, 787
BseMII CTCAG 1 cut(s) 564
BseRI GAGGAG 1 cut(s) 430
BseXI GCAGC 4 cut(s) 250, 377, 483, 991
BsgI GTGCAG 3 cut(s) 216, 384, 1001
Bsh1236I CGCG 1 cut(s) 375
BshFI GGCC 1 cut(s) 1130
BshNI GGYRCC 1 cut(s) 305
BsiHKAI GWGCWC 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 786
BsiSI CCGG 4 cut(s) 332, 468, 703, 712
BslFI GGGAC 1 cut(s) 479
BslI CCNNNNNNNGG 4 cut(s) 31, 481, 718, 787
BsmAI GTCTC 1 cut(s) 417
BsmFI GGGAC 1 cut(s) 479
BsnI GGCC 1 cut(s) 1130
BsoBI CYCGRG 1 cut(s) 786
Bsp1286I GDGCHC 1 cut(s) 225
Bsp13I TCCGGA 1 cut(s) 331
Bsp1407I TGTACA 2 cut(s) 442, 900
Bsp143I GATC 6 cut(s) 44, 118, 328, 334, 913, 1021
Bsp1720I GCTNAGC 1 cut(s) 15
Bsp19I CCATGG 1 cut(s) 475
BspANI GGCC 1 cut(s) 1130
BspCNI CTCAG 1 cut(s) 563
BspEI TCCGGA 1 cut(s) 331
BspFNI CGCG 1 cut(s) 375
BspLI GGNNCC 3 cut(s) 307, 495, 1110
BspMI ACCTGC 3 cut(s) 156, 595, 1077
BspPI GGATC 3 cut(s) 323, 342, 1016
BspT107I GGYRCC 1 cut(s) 305
BsrFI RCCGGY 1 cut(s) 467
BsrGI TGTACA 2 cut(s) 442, 900
BssAI RCCGGY 1 cut(s) 467
BssECI CCNNGG 4 cut(s) 126, 475, 555, 1112
BssMI GATC 6 cut(s) 44, 118, 328, 334, 913, 1021
BssT1I CCWWGG 3 cut(s) 126, 475, 555
Bst2UI CCWGG 3 cut(s) 26, 803, 1113
Bst4CI ACNGT 2 cut(s) 113, 447
Bst6I CTCTTC 2 cut(s) 269, 711
BstAPI GCANNNNNTGC 1 cut(s) 70
BstAUI TGTACA 2 cut(s) 442, 900
BstC8I GCNNGC 3 cut(s) 363, 469, 606
BstDEI CTNAG 2 cut(s) 15, 550
BstDSI CCRYGG 1 cut(s) 475
BstF5I GGATG 5 cut(s) 258, 486, 916, 928, 984
BstFNI CGCG 1 cut(s) 375
BstHHI GCGC 1 cut(s) 844
BstKTI GATC 6 cut(s) 47, 121, 331, 337, 916, 1024
BstMAI GTCTC 1 cut(s) 417
BstMBI GATC 6 cut(s) 44, 118, 328, 334, 913, 1021
BstMWI GCNNNNNNNGC 3 cut(s) 70, 137, 511
BstNI CCWGG 3 cut(s) 26, 803, 1113
BstNSI RCATGY 1 cut(s) 577
BstSCI CCNGG 4 cut(s) 24, 711, 801, 1111
BstSFI CTRYAG 1 cut(s) 819
BstUI CGCG 1 cut(s) 375
BstV1I GCAGC 4 cut(s) 250, 377, 483, 991
BstV2I GAAGAC 2 cut(s) 972, 1071
BsuRI GGCC 1 cut(s) 1130
BtgI CCRYGG 1 cut(s) 475
BtrI CACGTC 1 cut(s) 535
BtsCI GGATG 5 cut(s) 258, 486, 916, 928, 984
BtsIMutI CAGTG 2 cut(s) 118, 415
BveI ACCTGC 3 cut(s) 156, 595, 1077
Cac8I GCNNGC 3 cut(s) 363, 469, 606
CfoI GCGC 1 cut(s) 844
Cfr10I RCCGGY 1 cut(s) 467
Cfr13I GGNCC 4 cut(s) 493, 709, 799, 1109
Csp6I GTAC 4 cut(s) 84, 371, 443, 901
CviAII CATG 6 cut(s) 152, 210, 346, 476, 574, 839
CviQI GTAC 4 cut(s) 84, 371, 443, 901
DdeI CTNAG 2 cut(s) 15, 550
DpnI GATC 6 cut(s) 46, 120, 330, 336, 915, 1023
DpnII GATC 6 cut(s) 44, 118, 328, 334, 913, 1021
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
Eam1104I CTCTTC 2 cut(s) 269, 711
EarI CTCTTC 2 cut(s) 269, 711
Eco130I CCWWGG 3 cut(s) 126, 475, 555
Eco47I GGWCC 4 cut(s) 493, 709, 799, 1109
Eco88I CYCGRG 1 cut(s) 786
EcoO109I RGGNCCY 2 cut(s) 799, 1109
EcoRI GAATTC 1 cut(s) 1117
EcoRII CCWGG 3 cut(s) 24, 801, 1111
EcoT14I CCWWGG 3 cut(s) 126, 475, 555
ErhI CCWWGG 3 cut(s) 126, 475, 555
FaeI CATG 6 cut(s) 155, 213, 349, 479, 577, 842
FaqI GGGAC 1 cut(s) 479
FatI CATG 6 cut(s) 151, 209, 345, 475, 573, 838
FauNDI CATATG 1 cut(s) 55
Fnu4HI GCNGC 4 cut(s) 264, 366, 472, 1005
FokI GGATG 5 cut(s) 265, 493, 923, 935, 971
Fsp4HI GCNGC 4 cut(s) 264, 366, 472, 1005
FspBI CTAG 2 cut(s) 236, 1138
GlaI GCGC 1 cut(s) 843
GluI GCNGC 4 cut(s) 264, 366, 472, 1005
GsuI CTGGAG 1 cut(s) 824
HaeIII GGCC 1 cut(s) 1130
HapII CCGG 4 cut(s) 332, 468, 703, 712
HhaI GCGC 1 cut(s) 844
Hin1II CATG 6 cut(s) 155, 213, 349, 479, 577, 842
Hin6I GCGC 1 cut(s) 842
HinP1I GCGC 1 cut(s) 842
HincII GTYRAC 1 cut(s) 1038
HindII GTYRAC 1 cut(s) 1038
HinfI GANTC 3 cut(s) 597, 681, 870
HpaII CCGG 4 cut(s) 332, 468, 703, 712
HphI GGTGA 7 cut(s) 128, 306, 581, 757, 770, 896, 952
Hpy166II GTNNAC 1 cut(s) 1038
Hpy188I TCNGA 3 cut(s) 42, 193, 1030
Hpy188III TCNNGA 2 cut(s) 332, 770
Hpy8I GTNNAC 1 cut(s) 1038
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 3 cut(s) 237, 650, 700
HpyCH4III ACNGT 2 cut(s) 113, 447
HpyCH4IV ACGT 2 cut(s) 534, 811
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 137, 511
HpyF3I CTNAG 2 cut(s) 15, 550
HpySE526I ACGT 2 cut(s) 534, 811
Hsp92II CATG 6 cut(s) 155, 213, 349, 479, 577, 842
HspAI GCGC 1 cut(s) 842
Kpn2I TCCGGA 1 cut(s) 331
KroI GCCGGC 1 cut(s) 467
KroNI GCCGGC 1 cut(s) 469
Kzo9I GATC 6 cut(s) 44, 118, 328, 334, 913, 1021
LmnI GCTCC 1 cut(s) 220
Lsp1109I GCAGC 4 cut(s) 250, 377, 483, 991
LweI GCATC 5 cut(s) 140, 348, 511, 696, 919
MaeI CTAG 2 cut(s) 236, 1138
MaeII ACGT 2 cut(s) 534, 811
MaeIII GTNAC 4 cut(s) 535, 569, 1047, 1089
MalI GATC 6 cut(s) 46, 120, 330, 336, 915, 1023
MboI GATC 6 cut(s) 44, 118, 328, 334, 913, 1021
MboII GAAGA 6 cut(s) 286, 728, 749, 938, 972, 1071
MfeI CAATTG 1 cut(s) 1132
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 4 cut(s) 877, 1101, 1117, 1132
MmeI TCCRAC 2 cut(s) 419, 612
MroI TCCGGA 1 cut(s) 331
MroNI GCCGGC 1 cut(s) 467
MseI TTAA 2 cut(s) 171, 449
MslI CAYNNNNRTG 1 cut(s) 692
MspI CCGG 4 cut(s) 332, 468, 703, 712
MspR9I CCNGG 4 cut(s) 26, 713, 803, 1113
MunI CAATTG 1 cut(s) 1132
MvaI CCWGG 3 cut(s) 26, 803, 1113
MvnI CGCG 1 cut(s) 375
MwoI GCNNNNNNNGC 3 cut(s) 70, 137, 511
NaeI GCCGGC 1 cut(s) 469
NciI CCSGG 1 cut(s) 713
NcoI CCATGG 1 cut(s) 475
NdeI CATATG 1 cut(s) 55
NdeII GATC 6 cut(s) 44, 118, 328, 334, 913, 1021
NgoMIV GCCGGC 1 cut(s) 467
NlaIII CATG 6 cut(s) 155, 213, 349, 479, 577, 842
NlaIV GGNNCC 3 cut(s) 307, 495, 1110
NmuCI GTSAC 3 cut(s) 535, 569, 1089
NspI RCATGY 1 cut(s) 577
PdiI GCCGGC 1 cut(s) 469
PfeI GAWTC 3 cut(s) 597, 681, 870
PflMI CCANNNNNTGG 1 cut(s) 481
PfoI TCCNGGA 2 cut(s) 711, 801
PkrI GCNGC 4 cut(s) 265, 367, 473, 1006
PpuMI RGGWCCY 2 cut(s) 799, 1109
Psp5II RGGWCCY 2 cut(s) 799, 1109
Psp6I CCWGG 3 cut(s) 24, 801, 1111
PspGI CCWGG 3 cut(s) 24, 801, 1111
PspN4I GGNNCC 3 cut(s) 307, 495, 1110
PspPI GGNCC 4 cut(s) 493, 709, 799, 1109
PspPPI RGGWCCY 2 cut(s) 799, 1109
RsaI GTAC 4 cut(s) 85, 372, 444, 902
RsaNI GTAC 4 cut(s) 84, 371, 443, 901
RseI CAYNNNNRTG 1 cut(s) 692
SaqAI TTAA 2 cut(s) 171, 449
SatI GCNGC 4 cut(s) 264, 366, 472, 1005
Sau3AI GATC 6 cut(s) 44, 118, 328, 334, 913, 1021
Sau96I GGNCC 4 cut(s) 493, 709, 799, 1109
ScrFI CCNGG 4 cut(s) 26, 713, 803, 1113
SduI GDGCHC 1 cut(s) 225
SfaNI GCATC 5 cut(s) 140, 348, 511, 696, 919
SfcI CTRYAG 1 cut(s) 819
SinI GGWCC 4 cut(s) 493, 709, 799, 1109
SmiMI CAYNNNNRTG 1 cut(s) 692
Sse9I AATT 4 cut(s) 877, 1101, 1117, 1132
SspI AATATT 2 cut(s) 389, 851
SspMI CTAG 2 cut(s) 236, 1138
StyD4I CCNGG 4 cut(s) 24, 711, 801, 1111
StyI CCWWGG 3 cut(s) 126, 475, 555
TaaI ACNGT 2 cut(s) 113, 447
TaiI ACGT 2 cut(s) 537, 814
TaqI TCGA 2 cut(s) 916, 1082
TasI AATT 4 cut(s) 877, 1101, 1117, 1132
TatI WGTACW 3 cut(s) 83, 442, 900
TfiI GAWTC 3 cut(s) 597, 681, 870
Tru1I TTAA 2 cut(s) 171, 449
Tru9I TTAA 2 cut(s) 171, 449
TscAI CASTG 2 cut(s) 118, 415
TseFI GTSAC 3 cut(s) 535, 569, 1089
TseI GCWGC 4 cut(s) 263, 365, 471, 1004
Tsp45I GTSAC 3 cut(s) 535, 569, 1089
TspDTI ATGAA 4 cut(s) 198, 272, 334, 939
TspGWI ACGGA 1 cut(s) 935
TspRI CASTG 2 cut(s) 118, 415
Van91I CCANNNNNTGG 1 cut(s) 481
VpaK11BI GGWCC 4 cut(s) 493, 709, 799, 1109
XapI RAATTY 2 cut(s) 1101, 1117
XceI RCATGY 1 cut(s) 577
XcmI CCANNNNNNNNNTGG 1 cut(s) 822
XspI CTAG 2 cut(s) 236, 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.