RchiOBHm_Chr1g0383111

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0006698
Physical Location & Seq
Reverse (-)
67590880 .. 67594643
3764 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1332 bp
ATGACGAAGATGATGAAGGGTAGGGTGTCAAGAATTGGAAGCTATGCAATTGCTTCTTCCATTAAAGATGGCCATCATCAACAGCCTTGCATTACCTGCACAACATTCAACATACTCGCTCCAATTTACAAGCGCCTAAGCCATGAGGACCCAGATTGTAGGGAAAGCGATCACAGAGCTTATTGGCTGGCCAGGAATCACATGATTTTGGATTGTTTGTTGGCTGAGAGATCTTCCATTATTTGTCTTCAGGAATTCTGGGTTGGGAATGAAGAGCTAGTGAACATTTATGAGAAGAGACTTGGTGATGCTGGTTATATCAATTTTAAGCTAGGGAGGACCAACAACCGCGGCGATGGTTTGCTGACCGCGGTGCATAAGGACTACTTTAGAGTTGTAAATTATCAAGAGTTGCACTTCCATGATTGTGGAGATCGTGTTGCTCAGTTGTTACATGTTGAATTAGCTTGCCCAATTTCACAGTTTCAAAACAATGATATTCGGCAGGAAATGCTTATTGTGAATACCCATTTGTTATTTCCTCATGATTCAAGTTTGTGTATAGTACGATTGCATCAGGTGTACAAAATCCTACAATATGTGGAATCTTATCAGAAAGAGAAAAAACTTAACCCCATGCCTATCATTCTCTGTGGTGACTGGAATGGAAGCAAACGTGGACATGTTTACAAGTTTCTTAGGTCCCAGGGGTTTGTATCTTCTTATGATACTGCTCATCATTACACCGATGCAGATGCACAAAAGTGGGTTAGCCACCGAAACCATCGGGGAAACATATGTGGGGTTGACTTTATATGGCTTCTCAACCCAAACAGTTACCGGAAACTGCTAAAAACAAGTTGGAGTGAAGCAGTATTTGGCATGTTCAAGTATCTACTACGAAGAGCTTCACTGACAGAAAGTGATGCCTTTGCCTTTCTGAAGGCTGATAACCCTGGTGATTACATTACCTATTCTGGCTTCTGTGAAGCCCTCCGACAGCTTAATTTGACTGGTCATTGCCATGGACTTAGCCTTGAAGAGACCAAGGATTTGTGGGTTCAAGCAGACATAGATGGCAATGGTATTCTTGATTACAGAGGGTTTCAGCAGCGAATTTGGAATCCAACAGATGGTGGTAATGACATCTGGGATGTTGGCTCCAATGGTATTCAGGATCAAGCAATTGGTTTCAGTATAAAAAATGCAGTTCTTTTCCCTCCCGAAGCGGAGAAAGGAACGTGGCCCGAAGACTACTCTCTTTCTGATCATGCACGACTCACTGTGGTTTTCTCACCAGTAAGAATGCCATGCTCTCAGATGATCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

443

Amino Acids

50.86

Weight (kDa)

6.69

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 35 - 321 1.6e-08 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 104
AccB7I CCANNNNNTGG 1 cut(s) 1133
AccII CGCG 2 cut(s) 351, 371
AciI CCGC 5 cut(s) 349, 351, 369, 371, 1229
AclWI GGATC 1 cut(s) 1185
AcoI YGGCCR 2 cut(s) 70, 189
AcsI RAATTY 2 cut(s) 254, 1116
AcuI CTGAAG 2 cut(s) 233, 962
AfaI GTAC 2 cut(s) 567, 584
AfiI CCNNNNNNNGG 1 cut(s) 1133
AflIII ACRYGT 2 cut(s) 454, 682
AgsI TTSAA 7 cut(s) 109, 461, 488, 552, 889, 1040, 1064
AjnI CCWGG 3 cut(s) 191, 705, 955
AjuI GAANNNNNNNTTGG 4 cut(s) 246, 278, 861, 893
AleI CACNNNNGTG 1 cut(s) 763
AluBI AGCT 7 cut(s) 42, 179, 277, 331, 467, 908, 1003
AluI AGCT 7 cut(s) 42, 179, 277, 331, 467, 908, 1003
Alw26I GTCTC 2 cut(s) 292, 1037
AlwI GGATC 1 cut(s) 1185
AoxI GGCC 3 cut(s) 70, 189, 1244
ApeKI GCWGC 1 cut(s) 1111
ApoI RAATTY 2 cut(s) 254, 1116
ArsI GACNNNNNNTTYG 2 cut(s) 1036, 1068
Asp700I GAANNNNTTC 1 cut(s) 907
AspLEI GCGC 1 cut(s) 135
AspS9I GGNCC 4 cut(s) 148, 339, 702, 1245
AsuHPI GGTGA 4 cut(s) 317, 668, 971, 1287
AvaII GGWCC 3 cut(s) 148, 339, 702
BalI TGGCCA 2 cut(s) 72, 191
BbsI GAAGAC 2 cut(s) 239, 1257
BbvI GCAGC 1 cut(s) 1123
BccI CCATC 6 cut(s) 62, 81, 350, 792, 1070, 1127
BcgI CGANNNNNNTGC 2 cut(s) 737, 771
BciT130I CCWGG 3 cut(s) 193, 707, 957
BclI TGATCA 2 cut(s) 1267, 1323
BcoDI GTCTC 2 cut(s) 292, 1037
BfaI CTAG 2 cut(s) 278, 332
BfoI RGCGCY 1 cut(s) 136
BfuAI ACCTGC 1 cut(s) 104
BglII AGATCT 1 cut(s) 230
BisI GCNGC 2 cut(s) 352, 1112
BlsI GCNGC 2 cut(s) 353, 1113
Bme1390I CCNGG 3 cut(s) 193, 707, 957
Bme18I GGWCC 3 cut(s) 148, 339, 702
BmgT120I GGNCC 4 cut(s) 148, 339, 702, 1245
BmiI GGNNCC 3 cut(s) 150, 704, 1162
BmrFI CCNGG 3 cut(s) 193, 707, 957
BmsI GCATC 5 cut(s) 298, 583, 739, 745, 916
BpiI GAAGAC 2 cut(s) 239, 1257
Bpu10I CCTNAGC 1 cut(s) 137
BsaBI GATNNNNATC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 1037
BsaJI CCNNGG 7 cut(s) 349, 369, 705, 706, 955, 1024, 1047
BsaWI WCCGGW 1 cut(s) 840
BsaXI ACNNNNNCTCC 2 cut(s) 423, 453
Bsc4I CCNNNNNNNGG 1 cut(s) 1133
Bse1I ACTGG 3 cut(s) 665, 1018, 1298
Bse3DI GCAATG 2 cut(s) 1018, 1087
Bse8I GATNNNNATC 1 cut(s) 72
BseBI CCWGG 3 cut(s) 193, 707, 957
BseDI CCNNGG 7 cut(s) 349, 369, 705, 706, 955, 1024, 1047
BseGI GGATG 1 cut(s) 1159
BseJI GATNNNNATC 1 cut(s) 72
BseLI CCNNNNNNNGG 1 cut(s) 1133
BseMI GCAATG 2 cut(s) 1018, 1087
BseMII CTCAG 3 cut(s) 216, 458, 1331
BseNI ACTGG 3 cut(s) 665, 1018, 1298
BseXI GCAGC 1 cut(s) 1123
BsgI GTGCAG 1 cut(s) 82
Bsh1236I CGCG 2 cut(s) 351, 371
BshFI GGCC 3 cut(s) 72, 191, 1246
BsiSI CCGG 1 cut(s) 841
BslFI GGGAC 1 cut(s) 688
BslI CCNNNNNNNGG 1 cut(s) 1133
BsmAI GTCTC 2 cut(s) 292, 1037
BsmFI GGGAC 1 cut(s) 688
BsmI GAATGC 1 cut(s) 1311
BsnI GGCC 3 cut(s) 72, 191, 1246
Bso31I GGTCTC 1 cut(s) 1037
Bsp1407I TGTACA 1 cut(s) 582
Bsp143I GATC 6 cut(s) 169, 230, 433, 1177, 1267, 1323
Bsp19I CCATGG 1 cut(s) 1024
BspACI CCGC 5 cut(s) 349, 351, 369, 371, 1229
BspANI GGCC 3 cut(s) 72, 191, 1246
BspCNI CTCAG 3 cut(s) 217, 457, 1330
BspFNI CGCG 2 cut(s) 351, 371
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 3 cut(s) 150, 704, 1162
BspMI ACCTGC 1 cut(s) 104
BspPI GGATC 1 cut(s) 1185
BspQI GCTCTTC 2 cut(s) 267, 898
BspTNI GGTCTC 1 cut(s) 1037
BsrDI GCAATG 2 cut(s) 1018, 1087
BsrGI TGTACA 1 cut(s) 582
BsrI ACTGG 3 cut(s) 665, 1018, 1298
BssECI CCNNGG 7 cut(s) 349, 369, 705, 706, 955, 1024, 1047
BssMI GATC 6 cut(s) 169, 230, 433, 1177, 1267, 1323
BssT1I CCWWGG 2 cut(s) 1024, 1047
Bst2UI CCWGG 3 cut(s) 193, 707, 957
Bst4CI ACNGT 3 cut(s) 483, 836, 1285
Bst6I CTCTTC 4 cut(s) 267, 290, 898, 1035
BstAPI GCANNNNNTGC 2 cut(s) 96, 511
BstAUI TGTACA 1 cut(s) 582
BstC8I GCNNGC 2 cut(s) 189, 469
BstDEI CTNAG 6 cut(s) 137, 225, 444, 698, 1031, 1317
BstDSI CCRYGG 3 cut(s) 349, 369, 1024
BstF5I GGATG 1 cut(s) 1159
BstFNI CGCG 2 cut(s) 351, 371
BstH2I RGCGCY 1 cut(s) 136
BstHHI GCGC 1 cut(s) 135
BstKTI GATC 6 cut(s) 172, 233, 436, 1180, 1270, 1326
BstMAI GTCTC 2 cut(s) 292, 1037
BstMBI GATC 6 cut(s) 169, 230, 433, 1177, 1267, 1323
BstMWI GCNNNNNNNGC 2 cut(s) 96, 511
BstNI CCWGG 3 cut(s) 193, 707, 957
BstNSI RCATGY 3 cut(s) 458, 686, 886
BstSCI CCNGG 3 cut(s) 191, 705, 955
BstUI CGCG 2 cut(s) 351, 371
BstV1I GCAGC 1 cut(s) 1123
BstV2I GAAGAC 2 cut(s) 239, 1257
BstX2I RGATCY 1 cut(s) 230
BstXI CCANNNNNNTGG 1 cut(s) 428
BstYI RGATCY 1 cut(s) 230
BsuRI GGCC 3 cut(s) 72, 191, 1246
BtgI CCRYGG 3 cut(s) 349, 369, 1024
BtgZI GCGATG 1 cut(s) 369
BtsCI GGATG 1 cut(s) 1159
BtsIMutI CAGTG 2 cut(s) 911, 1281
BveI ACCTGC 1 cut(s) 104
Cac8I GCNNGC 2 cut(s) 189, 469
CciI TCATGA 1 cut(s) 544
CfoI GCGC 1 cut(s) 135
Cfr13I GGNCC 4 cut(s) 148, 339, 702, 1245
Cfr42I CCGCGG 2 cut(s) 352, 372
Csp6I GTAC 2 cut(s) 566, 583
CviQI GTAC 2 cut(s) 566, 583
DdeI CTNAG 6 cut(s) 137, 225, 444, 698, 1031, 1317
DpnI GATC 6 cut(s) 171, 232, 435, 1179, 1269, 1325
DpnII GATC 6 cut(s) 169, 230, 433, 1177, 1267, 1323
EaeI YGGCCR 2 cut(s) 70, 189
Eam1104I CTCTTC 4 cut(s) 267, 290, 898, 1035
EarI CTCTTC 4 cut(s) 267, 290, 898, 1035
Eco130I CCWWGG 2 cut(s) 1024, 1047
Eco31I GGTCTC 1 cut(s) 1037
Eco47I GGWCC 3 cut(s) 148, 339, 702
Eco57I CTGAAG 2 cut(s) 233, 962
EcoO109I RGGNCCY 2 cut(s) 148, 702
EcoRI GAATTC 1 cut(s) 254
EcoRII CCWGG 3 cut(s) 191, 705, 955
EcoT14I CCWWGG 2 cut(s) 1024, 1047
ErhI CCWWGG 2 cut(s) 1024, 1047
FalI AAGNNNNNCTT 2 cut(s) 371, 403
FaqI GGGAC 1 cut(s) 688
FauNDI CATATG 1 cut(s) 797
FbaI TGATCA 2 cut(s) 1267, 1323
Fnu4HI GCNGC 2 cut(s) 352, 1112
FokI GGATG 1 cut(s) 1166
Fsp4HI GCNGC 2 cut(s) 352, 1112
FspBI CTAG 2 cut(s) 278, 332
GlaI GCGC 1 cut(s) 134
GluI GCNGC 2 cut(s) 352, 1112
HaeII RGCGCY 1 cut(s) 136
HaeIII GGCC 3 cut(s) 72, 191, 1246
HapII CCGG 1 cut(s) 841
HhaI GCGC 1 cut(s) 135
Hin6I GCGC 1 cut(s) 133
HinP1I GCGC 1 cut(s) 133
HincII GTYRAC 1 cut(s) 808
HindII GTYRAC 1 cut(s) 808
HinfI GANTC 5 cut(s) 196, 548, 605, 1123, 1278
HpaII CCGG 1 cut(s) 841
HphI GGTGA 4 cut(s) 317, 668, 971, 1287
Hpy166II GTNNAC 5 cut(s) 283, 583, 680, 688, 808
Hpy188I TCNGA 5 cut(s) 615, 942, 998, 1267, 1320
Hpy188III TCNNGA 7 cut(s) 30, 251, 407, 545, 1091, 1175, 1223
Hpy8I GTNNAC 5 cut(s) 283, 583, 680, 688, 808
HpyAV CCTTC 2 cut(s) 10, 937
HpyCH4III ACNGT 3 cut(s) 483, 836, 1285
HpyCH4IV ACGT 2 cut(s) 676, 1241
HpyF10VI GCNNNNNNNGC 2 cut(s) 96, 511
HpyF3I CTNAG 6 cut(s) 137, 225, 444, 698, 1031, 1317
HpySE526I ACGT 2 cut(s) 676, 1241
HspAI GCGC 1 cut(s) 133
Ksp22I TGATCA 2 cut(s) 1267, 1323
KspI CCGCGG 2 cut(s) 352, 372
Kzo9I GATC 6 cut(s) 169, 230, 433, 1177, 1267, 1323
LguI GCTCTTC 2 cut(s) 267, 898
LmnI GCTCC 2 cut(s) 124, 1166
Lsp1109I GCAGC 1 cut(s) 1123
LweI GCATC 5 cut(s) 298, 583, 739, 745, 916
MaeI CTAG 2 cut(s) 278, 332
MaeII ACGT 2 cut(s) 676, 1241
MaeIII GTNAC 3 cut(s) 450, 656, 836
MalI GATC 6 cut(s) 171, 232, 435, 1179, 1269, 1325
MboI GATC 6 cut(s) 169, 230, 433, 1177, 1267, 1323
MfeI CAATTG 2 cut(s) 48, 1185
MflI RGATCY 1 cut(s) 230
MlsI TGGCCA 2 cut(s) 72, 191
MluNI TGGCCA 2 cut(s) 72, 191
MlyI GAGTC 1 cut(s) 1272
MmeI TCCRAC 3 cut(s) 842, 1021, 1151
MnlI CCTC 6 cut(s) 139, 330, 552, 1004, 1094, 1230
Mox20I TGGCCA 2 cut(s) 72, 191
MroXI GAANNNNTTC 1 cut(s) 907
MscI TGGCCA 2 cut(s) 72, 191
MseI TTAA 4 cut(s) 63, 327, 630, 1005
MslI CAYNNNNRTG 4 cut(s) 420, 426, 763, 1023
Msp20I TGGCCA 2 cut(s) 72, 191
MspA1I CMGCKG 2 cut(s) 351, 371
MspI CCGG 1 cut(s) 841
MspR9I CCNGG 3 cut(s) 193, 707, 957
MunI CAATTG 2 cut(s) 48, 1185
Mva1269I GAATGC 1 cut(s) 1311
MvaI CCWGG 3 cut(s) 193, 707, 957
MvnI CGCG 2 cut(s) 351, 371
MwoI GCNNNNNNNGC 2 cut(s) 96, 511
NcoI CCATGG 1 cut(s) 1024
NdeI CATATG 1 cut(s) 797
NdeII GATC 6 cut(s) 169, 230, 433, 1177, 1267, 1323
NlaIV GGNNCC 3 cut(s) 150, 704, 1162
NmuCI GTSAC 1 cut(s) 656
NspI RCATGY 3 cut(s) 458, 686, 886
OliI CACNNNNGTG 1 cut(s) 763
PagI TCATGA 1 cut(s) 544
PasI CCCWGGG 1 cut(s) 706
PciI ACATGT 2 cut(s) 454, 682
PciSI GCTCTTC 2 cut(s) 267, 898
PctI GAATGC 1 cut(s) 1311
PdmI GAANNNNTTC 1 cut(s) 907
PfeI GAWTC 4 cut(s) 196, 548, 605, 1123
PflMI CCANNNNNTGG 1 cut(s) 1133
PkrI GCNGC 2 cut(s) 353, 1113
PleI GAGTC 1 cut(s) 1272
PpsI GAGTC 1 cut(s) 1272
PpuMI RGGWCCY 2 cut(s) 148, 702
PscI ACATGT 2 cut(s) 454, 682
Psp5II RGGWCCY 2 cut(s) 148, 702
Psp6I CCWGG 3 cut(s) 191, 705, 955
PspGI CCWGG 3 cut(s) 191, 705, 955
PspN4I GGNNCC 3 cut(s) 150, 704, 1162
PspPI GGNCC 4 cut(s) 148, 339, 702, 1245
PspPPI RGGWCCY 2 cut(s) 148, 702
PsuI RGATCY 1 cut(s) 230
RsaI GTAC 2 cut(s) 567, 584
RsaNI GTAC 2 cut(s) 566, 583
RseI CAYNNNNRTG 4 cut(s) 420, 426, 763, 1023
SacII CCGCGG 2 cut(s) 352, 372
SapI GCTCTTC 2 cut(s) 267, 898
SaqAI TTAA 4 cut(s) 63, 327, 630, 1005
SatI GCNGC 2 cut(s) 352, 1112
Sau3AI GATC 6 cut(s) 169, 230, 433, 1177, 1267, 1323
Sau96I GGNCC 4 cut(s) 148, 339, 702, 1245
SchI GAGTC 1 cut(s) 1272
ScrFI CCNGG 3 cut(s) 193, 707, 957
SfaNI GCATC 5 cut(s) 298, 583, 739, 745, 916
Sfr303I CCGCGG 2 cut(s) 352, 372
SgrBI CCGCGG 2 cut(s) 352, 372
SinI GGWCC 3 cut(s) 148, 339, 702
SmiMI CAYNNNNRTG 4 cut(s) 420, 426, 763, 1023
SsiI CCGC 5 cut(s) 349, 351, 369, 371, 1229
SspMI CTAG 2 cut(s) 278, 332
StyD4I CCNGG 3 cut(s) 191, 705, 955
StyI CCWWGG 2 cut(s) 1024, 1047
TaaI ACNGT 3 cut(s) 483, 836, 1285
TaiI ACGT 2 cut(s) 679, 1244
TatI WGTACW 1 cut(s) 582
TauI GCSGC 1 cut(s) 354
TfiI GAWTC 4 cut(s) 196, 548, 605, 1123
Tru1I TTAA 4 cut(s) 63, 327, 630, 1005
Tru9I TTAA 4 cut(s) 63, 327, 630, 1005
TscAI CASTG 2 cut(s) 918, 1288
TseFI GTSAC 1 cut(s) 656
TseI GCWGC 1 cut(s) 1111
Tsp45I GTSAC 1 cut(s) 656
TspDTI ATGAA 2 cut(s) 29, 285
TspRI CASTG 2 cut(s) 918, 1288
Van91I CCANNNNNTGG 1 cut(s) 1133
VpaK11BI GGWCC 3 cut(s) 148, 339, 702
XapI RAATTY 2 cut(s) 254, 1116
XceI RCATGY 3 cut(s) 458, 686, 886
XmnI GAANNNNTTC 1 cut(s) 907
XspI CTAG 2 cut(s) 278, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.