RchiOBHm_Chr2g0126191

CAAX protease self-immunity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0011002
Physical Location & Seq
Reverse (-)
40349629 .. 40354324
4696 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49820

Sequence Viewer

Length: 942 bp
ATGATAAAGTTACAGTGCCATCTGAAGCTGATGTATCCAACCACACCTACAAAATTATCCATTGGGGATTTTACGTCAAAACTCAAACTCCCGTCACCCTCTCCGTCTCCATCTCCTGGTGCTCTTCTCCTTTGCACGAATTTCAAATCCAGCTTAACCTCAAAACCAGATTCTATCAAGTGCTTTTGTAACAAAACTGACAACACCCAACAAGTCTCTCAGGGCTTCTCAGCACTGGCGGCAGATAGTCCATGGGACGTTGCAACTGTGTGGAGCACTCTGGCTTTCTATATCTTCAGTTTGCACATTCCTCTTAGTTTTGGAGGTTTGTCTGTGGTTGCTTATGTACTGAATCAGCCTGTTCTTGACCCACAGATTGAGGCAATATCGTTGCTTGGAGCTCAGTTTCTAGAGCTCATTGCAGCAATGCTTCTTCTGCAGAGTACTGCTAAGCCACAGTATAAGTTTGCGAACTTCTTCAAAGCTAACAAATTGTCAAAAGAGAGGAGCTGGCTGTTGGTATCAGCTGTAGGATTTGGGTTTCTCTTTGTGTTGGTTTTCCTTACGTCTTTTCTTGCTGATAGATTCATTGGTCCAAAGGATATAAACAACCCTGTACTGAAGGAAATCCTGCTAAGCAGCGACATCTCAAAAGCTGCATGTTTCTTTGTATACTGCGTTATTACTCCCCTGCTGGAAGAGACTGTTTACCGAGGTTTTTTATTGACATCAATGTCATCCACCATGAAATGGCAGTCAGCTGTTTTGATAAGCTCAGCTATATTTAGTGCAGCTCACTTCTCTGGTGAGAATTTCCTACAGTTGTTTATTATTGGAAGTGTCCTTGGATGCTCTTATTGTTGGACTGGAAACTTGGGTTCCTCCATTTTAATACATTCCCTGTACAACGCCCTGACCCTAATGGTAACATTCTTATCCTAA

Protein Analysis

313

Amino Acids

34.29

Weight (kDa)

8.72

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 219 - 305 1.1e-20 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 733
AccB7I CCANNNNNTGG 2 cut(s) 116, 750
AccI GTMKAC 1 cut(s) 672
AciI CCGC 1 cut(s) 239
AcsI RAATTY 2 cut(s) 139, 811
AcuI CTGAAG 3 cut(s) 44, 280, 641
AfaI GTAC 4 cut(s) 348, 445, 618, 905
AfiI CCNNNNNNNGG 2 cut(s) 116, 750
AgsI TTSAA 2 cut(s) 145, 481
AjnI CCWGG 1 cut(s) 115
Alw21I GWGCWC 4 cut(s) 124, 278, 403, 417
Alw26I GTCTC 3 cut(s) 111, 220, 695
ApeKI GCWGC 4 cut(s) 422, 639, 656, 791
ApoI RAATTY 2 cut(s) 139, 811
Asp700I GAANNNNTTC 1 cut(s) 476
AspS9I GGNCC 1 cut(s) 593
AsuHPI GGTGA 2 cut(s) 87, 818
AvaII GGWCC 1 cut(s) 593
BanII GRGCYC 2 cut(s) 403, 417
Bbv12I GWGCWC 4 cut(s) 124, 278, 403, 417
BbvI GCAGC 4 cut(s) 434, 643, 651, 803
BccI CCATC 2 cut(s) 27, 118
BciT130I CCWGG 1 cut(s) 117
BciVI GTATCC 1 cut(s) 45
BcoDI GTCTC 3 cut(s) 111, 220, 695
BfaI CTAG 1 cut(s) 410
BfmI CTRYAG 3 cut(s) 437, 528, 818
BfuI GTATCC 1 cut(s) 45
BisI GCNGC 5 cut(s) 240, 423, 640, 657, 792
BlpI GCTNAGC 3 cut(s) 450, 635, 775
BlsI GCNGC 5 cut(s) 241, 424, 641, 658, 793
BmcAI AGTACT 1 cut(s) 445
Bme1390I CCNGG 1 cut(s) 117
Bme18I GGWCC 1 cut(s) 593
BmgT120I GGNCC 1 cut(s) 593
BmiI GGNNCC 1 cut(s) 880
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 1 cut(s) 839
Bpu1102I GCTNAGC 3 cut(s) 450, 635, 775
BsaJI CCNNGG 3 cut(s) 251, 712, 844
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 750
Bse1I ACTGG 2 cut(s) 240, 871
Bse3DI GCAATG 2 cut(s) 417, 432
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 3 cut(s) 251, 712, 844
BseGI GGATG 2 cut(s) 737, 854
BseLI CCNNNNNNNGG 2 cut(s) 116, 750
BseMI GCAATG 2 cut(s) 417, 432
BseMII CTCAG 4 cut(s) 233, 243, 416, 789
BseNI ACTGG 2 cut(s) 240, 871
BseRI GAGGAG 1 cut(s) 520
BseXI GCAGC 4 cut(s) 434, 643, 651, 803
BsgI GTGCAG 1 cut(s) 810
BsiHKAI GWGCWC 4 cut(s) 124, 278, 403, 417
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 2 cut(s) 116, 750
BsmAI GTCTC 3 cut(s) 111, 220, 695
BsmBI CGTCTC 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 269
Bsp1286I GDGCHC 4 cut(s) 124, 278, 403, 417
Bsp1407I TGTACA 1 cut(s) 903
Bsp1720I GCTNAGC 3 cut(s) 450, 635, 775
Bsp19I CCATGG 1 cut(s) 251
BspACI CCGC 1 cut(s) 239
BspCNI CTCAG 4 cut(s) 232, 242, 415, 788
BspLI GGNNCC 1 cut(s) 880
BspMAI CTGCAG 1 cut(s) 441
BspQI GCTCTTC 1 cut(s) 129
BsrDI GCAATG 2 cut(s) 417, 432
BsrGI TGTACA 1 cut(s) 903
BsrI ACTGG 2 cut(s) 240, 871
BssECI CCNNGG 3 cut(s) 251, 712, 844
BssNAI GTATAC 1 cut(s) 673
BssT1I CCWWGG 2 cut(s) 251, 844
Bst1107I GTATAC 1 cut(s) 673
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 5 cut(s) 15, 268, 459, 706, 822
Bst6I CTCTTC 2 cut(s) 129, 693
BstAUI TGTACA 1 cut(s) 903
BstC8I GCNNGC 1 cut(s) 512
BstDEI CTNAG 7 cut(s) 219, 229, 314, 402, 450, 635, 775
BstDSI CCRYGG 1 cut(s) 251
BstF5I GGATG 2 cut(s) 737, 854
BstMAI GTCTC 3 cut(s) 111, 220, 695
BstMWI GCNNNNNNNGC 2 cut(s) 239, 436
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 663
BstSCI CCNGG 1 cut(s) 115
BstSFI CTRYAG 3 cut(s) 437, 528, 818
BstV1I GCAGC 4 cut(s) 434, 643, 651, 803
BstZ17I GTATAC 1 cut(s) 673
BsuI GTATCC 1 cut(s) 45
BtgI CCRYGG 1 cut(s) 251
BtsCI GGATG 2 cut(s) 737, 854
BtsIMutI CAGTG 2 cut(s) 20, 233
Cac8I GCNNGC 1 cut(s) 512
Cfr13I GGNCC 1 cut(s) 593
Csp6I GTAC 4 cut(s) 347, 444, 617, 904
CviAII CATG 3 cut(s) 252, 660, 745
CviQI GTAC 4 cut(s) 347, 444, 617, 904
DdeI CTNAG 7 cut(s) 219, 229, 314, 402, 450, 635, 775
DrdI GACNNNNNNGTC 1 cut(s) 733
DseDI GACNNNNNNGTC 1 cut(s) 733
Eam1104I CTCTTC 2 cut(s) 129, 693
EarI CTCTTC 2 cut(s) 129, 693
Ecl136II GAGCTC 2 cut(s) 401, 415
Eco130I CCWWGG 2 cut(s) 251, 844
Eco24I GRGCYC 2 cut(s) 403, 417
Eco47I GGWCC 1 cut(s) 593
Eco53kI GAGCTC 2 cut(s) 401, 415
Eco57I CTGAAG 3 cut(s) 44, 280, 641
EcoICRI GAGCTC 2 cut(s) 401, 415
EcoRII CCWGG 1 cut(s) 115
EcoT14I CCWWGG 2 cut(s) 251, 844
EcoT38I GRGCYC 2 cut(s) 403, 417
ErhI CCWWGG 2 cut(s) 251, 844
Esp3I CGTCTC 1 cut(s) 111
FaeI CATG 3 cut(s) 255, 663, 748
FaiI YATR 9 cut(s) 253, 291, 345, 462, 605, 661, 673, 746, 782
FaqI GGGAC 1 cut(s) 269
FatI CATG 3 cut(s) 251, 659, 744
FblI GTMKAC 1 cut(s) 672
Fnu4HI GCNGC 5 cut(s) 240, 423, 640, 657, 792
FokI GGATG 2 cut(s) 724, 861
FriOI GRGCYC 2 cut(s) 403, 417
Fsp4HI GCNGC 5 cut(s) 240, 423, 640, 657, 792
FspBI CTAG 1 cut(s) 410
GluI GCNGC 5 cut(s) 240, 423, 640, 657, 792
Hin1II CATG 3 cut(s) 255, 663, 748
HinfI GANTC 3 cut(s) 170, 352, 585
HphI GGTGA 2 cut(s) 87, 818
Hpy166II GTNNAC 2 cut(s) 673, 709
Hpy188I TCNGA 1 cut(s) 24
Hpy188III TCNNGA 2 cut(s) 365, 410
Hpy8I GTNNAC 2 cut(s) 673, 709
HpyAV CCTTC 1 cut(s) 616
HpyCH4III ACNGT 5 cut(s) 15, 268, 459, 706, 822
HpyCH4IV ACGT 3 cut(s) 74, 258, 566
HpyCH4V TGCA 7 cut(s) 135, 263, 304, 422, 439, 659, 791
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 436
HpyF3I CTNAG 7 cut(s) 219, 229, 314, 402, 450, 635, 775
HpySE526I ACGT 3 cut(s) 74, 258, 566
Hsp92II CATG 3 cut(s) 255, 663, 748
LguI GCTCTTC 1 cut(s) 129
LmnI GCTCC 3 cut(s) 273, 398, 507
Lsp1109I GCAGC 4 cut(s) 434, 643, 651, 803
LweI GCATC 1 cut(s) 839
MaeI CTAG 1 cut(s) 410
MaeII ACGT 3 cut(s) 74, 258, 566
MaeIII GTNAC 4 cut(s) 9, 93, 188, 925
MboII GAAGA 5 cut(s) 116, 286, 425, 469, 710
MhlI GDGCHC 4 cut(s) 124, 278, 403, 417
MluCI AATT 4 cut(s) 53, 139, 491, 811
MmeI TCCRAC 2 cut(s) 62, 842
MnlI CCTC 8 cut(s) 109, 169, 317, 321, 373, 498, 707, 892
MroXI GAANNNNTTC 1 cut(s) 476
MseI TTAA 2 cut(s) 155, 890
MspA1I CMGCKG 2 cut(s) 527, 761
MspR9I CCNGG 1 cut(s) 117
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 2 cut(s) 239, 436
NcoI CCATGG 1 cut(s) 251
NlaIII CATG 3 cut(s) 255, 663, 748
NlaIV GGNNCC 1 cut(s) 880
NmuCI GTSAC 1 cut(s) 93
NspI RCATGY 1 cut(s) 663
PciSI GCTCTTC 1 cut(s) 129
PdmI GAANNNNTTC 1 cut(s) 476
PfeI GAWTC 3 cut(s) 170, 352, 585
PflMI CCANNNNNTGG 2 cut(s) 116, 750
PkrI GCNGC 5 cut(s) 241, 424, 641, 658, 793
Psp124BI GAGCTC 2 cut(s) 403, 417
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 1 cut(s) 880
PspPI GGNCC 1 cut(s) 593
PstI CTGCAG 1 cut(s) 441
PvuII CAGCTG 2 cut(s) 527, 761
RsaI GTAC 4 cut(s) 348, 445, 618, 905
RsaNI GTAC 4 cut(s) 347, 444, 617, 904
SacI GAGCTC 2 cut(s) 403, 417
SapI GCTCTTC 1 cut(s) 129
SaqAI TTAA 2 cut(s) 155, 890
SatI GCNGC 5 cut(s) 240, 423, 640, 657, 792
Sau96I GGNCC 1 cut(s) 593
ScaI AGTACT 1 cut(s) 445
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 4 cut(s) 124, 278, 403, 417
SfaNI GCATC 1 cut(s) 839
SfcI CTRYAG 3 cut(s) 437, 528, 818
SinI GGWCC 1 cut(s) 593
Sse9I AATT 4 cut(s) 53, 139, 491, 811
SsiI CCGC 1 cut(s) 239
SspMI CTAG 1 cut(s) 410
SstI GAGCTC 2 cut(s) 403, 417
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 2 cut(s) 251, 844
TaaI ACNGT 5 cut(s) 15, 268, 459, 706, 822
TaiI ACGT 3 cut(s) 77, 261, 569
TasI AATT 4 cut(s) 53, 139, 491, 811
TatI WGTACW 4 cut(s) 346, 443, 616, 903
TauI GCSGC 1 cut(s) 242
TfiI GAWTC 3 cut(s) 170, 352, 585
Tru1I TTAA 2 cut(s) 155, 890
Tru9I TTAA 2 cut(s) 155, 890
TscAI CASTG 2 cut(s) 20, 240
TseFI GTSAC 1 cut(s) 93
TseI GCWGC 4 cut(s) 422, 639, 656, 791
Tsp45I GTSAC 1 cut(s) 93
TspDTI ATGAA 2 cut(s) 577, 761
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 2 cut(s) 20, 240
Van91I CCANNNNNTGG 2 cut(s) 116, 750
VpaK11BI GGWCC 1 cut(s) 593
XapI RAATTY 2 cut(s) 139, 811
XbaI TCTAGA 1 cut(s) 409
XceI RCATGY 1 cut(s) 663
XmiI GTMKAC 1 cut(s) 672
XmnI GAANNNNTTC 1 cut(s) 476
XspI CTAG 1 cut(s) 410
ZrmI AGTACT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.