RchiOBHm_Chr2g0169081

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004185
Physical Location & Seq
Forward (+)
83361116 .. 83367378
6263 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53671

Sequence Viewer

Length: 1944 bp
ATGTCGGACGTCCATCTCTTCAATAACATCTCTCTCGGCGGCCGCGGCGGCACTAATCCGGGGCAGCTAAAAATACATCCAGGAGGGATTATGTGGAAGAAACAAGGGGGTGGGAAAGCAATTGAGGTTGATAAGGCGGTCATTGTTGGAGTGACATGGATGAAGGTCCCAAAGACAAATCAACTTGGTGTTCGGATTAAAGATGGGTTGTACTATAAATTCATTGGATTTCGAGACCAGGATGTCACTACTTTAACCAATTATTTCCAAACTACTTTCGGAATAGTAGCAGAGGAGAAGAAGCTTTCTGTCAGTGGGCGTAACTGGGGAGAAGTTGATTTAAGCGATAATATGCTGTCTTTCTTGGTTGACTCCAAGCAAGGATTTGAGGTATCTTTGGCTGATGTCTCACAAACACAGCTTCAGGGGAAAAACGACGTCACCTTAGAGTTCCATGTGGATGACACCACTGGAGCAAACGAGAAAGATTCTTTGATGGAAGTAACTTTTCACATTCCCAACTCCAACACTCAATTTATGGGTGATGAGAATCGTCCTCCTGCTCAGGTATTTCGTGACAAAATTATGTCGATGGCAGATGTTGGTGCTGGAGGTGAAGATGCTGTTGTCACATTTGAGAGTATTGCAGTCCTCACACCAAGGGGTCGCTACAATGTTGAACTTCATCTTTCATTCTTGCGGTTGCAAGGGCAGGCTAATGATTTCAAAATTCAGTACAGCAGTGTTGTTCGCCTCTTTCTCCTTCCTAAGTCTAACCAGCCACATACCTTTGTTGTTGTTACTCTTGATCCGCCTATCCGTAAAGGACAAACACTGTATCCACACATTGTTTTGCAGTTTGAAACTGAATATGTAGTTGAAAGTGAACTGGCAATAAGTGATGACCTTTTAAACGGCAAGTACAAAGACAAGTTAGAACCAACTTATAAGGGACTAATTCATGATGTGTTCACCAAAATACTGCATGGATTGTCTGGTGCCAAAATTACTAAACCAGGGAAGTTCCGCAGCTGTCAAGATGGTTATGCTGTGAAGTCTTCACTGAAAGCTGAAGACGGAGTTCTATATCCTCTGGAAAAGAGCTTCTTTTTCCTACCTAAACCTCCTACCCTTATTACTCATGATGTGATTGACTATGTGGAATTTGAGAGGCATGGTGCTGGAGGCTCAAATATGCATTATTTTGATCTTCTTATCAGACTTAAAAGTGAGCAAGAACATCTGTTTAGAAACATTCAGAGGAATGAATATCATACTCTTTTTGACTTCATCAGTTTAAAGGGTATCAAAATCATGAACTTAGGAGATGCTCAAGTAACAGATGGTGTGTCTCCTCTTCTTGAAGAGGCAGATGATGATGCTGTTGTTGACCCACATCTTGTTCGCGTAAAGAATGAAGCTGGTGAGGAAGATAGTGATGAAGAGGATGAAGATTTTGTTATTGAGAAGGATGATGGAGGTTCTCCAACTGATGATTCTGGGGGTGAAGATTCTGATGCTAGTGAAAGTGGAGGCGAGAAAGAGAAGCCTGCTAAAAAGGAGCCCAGGAAAGAACCTTCAACTTCAAAGGTGCCATCTTCCAAGAAAAAATCCAAAGAGGTCGATGAAGATGGTCCAAAGAAGAAAAAACAGAAAAAGAAGAAGGATCCAAATGCACCAAAAAGAGCGATGTCTGGTTTCATGTTCTTCTCACAGATGGAGAGAGAGAATGTGAAGAAAGACAACCCCGGGATTGCATTTACGGATGTGGGAAGAGTGCTTGGGGACAAGTGGAAGAAGATGTCAGCGGAGGAGAAAGAACCATATGAAGCAAAGGCCCGTCAAGATAAATTACGCTACAAGGATGAAATTAGTGGCTACAACAAGAGTAACACCCCCCAACCCATGAATGTGGATTCAGGGAATGAATCTGACAGCGAATAG

Protein Analysis

647

Amino Acids

72.33

Weight (kDa)

5.47

Isoelectric Point (pI)

34.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
POB3_N PF17292 7 - 98 5.9e-23 POB3-like N-terminal PH domain
SSrecog PF03531 106 - 174 2.1e-24 Structure-specific recognition protein (SSRP1)
PH1_SSRP1-like PF21103 207 - 336 3.1e-50 SSRP1 PH domain
Rttp106-like_middle PF08512 349 - 440 7.5e-22 Histone chaperone Rttp106-like, middle domain
HMG_box_2 PF09011 557 - 627 3.1e-16 HMG-box domain
HMG_box PF00505 560 - 628 2e-21 HMG (high mobility group) box
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 948
AasI GACNNNNNNGTC 1 cut(s) 242
AatII GACGTC 2 cut(s) 12, 441
AccB1I GGYRCC 2 cut(s) 998, 1591
AccII CGCG 2 cut(s) 45, 1407
AciI CCGC 9 cut(s) 39, 43, 45, 48, 137, 700, 812, 1027, 1808
AclWI GGATC 3 cut(s) 803, 1661, 1674
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 3 cut(s) 218, 729, 1163
AcuI CTGAAG 2 cut(s) 407, 1092
AcyI GRCGYC 2 cut(s) 9, 438
AfaI GTAC 3 cut(s) 212, 737, 923
AgsI TTSAA 8 cut(s) 22, 680, 727, 863, 881, 1364, 1581, 1587
AjnI CCWGG 4 cut(s) 79, 237, 1015, 1565
AjuI GAANNNNNNNTTGG 2 cut(s) 1480, 1512
AluBI AGCT 7 cut(s) 67, 304, 421, 1032, 1070, 1104, 1421
AluI AGCT 7 cut(s) 67, 304, 421, 1032, 1070, 1104, 1421
Alw26I GTCTC 3 cut(s) 228, 412, 1356
AlwI GGATC 3 cut(s) 803, 1661, 1674
Ama87I CYCGRG 1 cut(s) 1748
AoxI GGCC 2 cut(s) 40, 1836
ApeKI GCWGC 2 cut(s) 64, 1029
ApoI RAATTY 3 cut(s) 218, 729, 1163
AspS9I GGNCC 3 cut(s) 166, 1634, 1837
AsuC2I CCSGG 3 cut(s) 60, 1749, 1750
AsuHPI GGTGA 6 cut(s) 433, 554, 626, 964, 1436, 1517
AvaI CYCGRG 1 cut(s) 1748
AvaII GGWCC 2 cut(s) 166, 1634
BamHI GGATCC 1 cut(s) 1666
BanI GGYRCC 2 cut(s) 998, 1591
BanII GRGCYC 1 cut(s) 1566
BbsI GAAGAC 2 cut(s) 1050, 1080
BbvI GCAGC 2 cut(s) 76, 1041
BceAI ACGGC 1 cut(s) 931
BciT130I CCWGG 4 cut(s) 81, 239, 1017, 1567
BciVI GTATCC 1 cut(s) 849
BcnI CCSGG 3 cut(s) 60, 1749, 1750
BcoDI GTCTC 3 cut(s) 228, 412, 1356
BfaI CTAG 1 cut(s) 1521
BfuI GTATCC 1 cut(s) 849
BglI GCCNNNNNGGC 1 cut(s) 48
BisI GCNGC 6 cut(s) 40, 43, 46, 49, 65, 1030
BlsI GCNGC 6 cut(s) 41, 44, 47, 50, 66, 1031
Bme1390I CCNGG 7 cut(s) 60, 81, 239, 1017, 1567, 1749, 1750
Bme18I GGWCC 2 cut(s) 166, 1634
BmeT110I CYCGRG 1 cut(s) 1748
BmgT120I GGNCC 3 cut(s) 166, 1634, 1837
BmiI GGNNCC 5 cut(s) 168, 1000, 1563, 1593, 1668
BmrFI CCNGG 7 cut(s) 60, 81, 239, 1017, 1567, 1749, 1750
BmrI ACTGGG 1 cut(s) 334
BmsI GCATC 4 cut(s) 610, 1318, 1369, 1507
BmuI ACTGGG 1 cut(s) 334
BpiI GAAGAC 2 cut(s) 1050, 1080
BpmI CTGGAG 3 cut(s) 492, 630, 1203
Bpu10I CCTNAGC 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 1317
BpuMI CCSGG 3 cut(s) 60, 1749, 1750
BsaBI GATNNNNATC 1 cut(s) 549
BsaHI GRCGYC 2 cut(s) 9, 438
BsaI GGTCTC 1 cut(s) 228
BsaJI CCNNGG 7 cut(s) 43, 59, 659, 1016, 1565, 1747, 1748
Bse1I ACTGG 3 cut(s) 329, 475, 894
Bse8I GATNNNNATC 1 cut(s) 549
BseBI CCWGG 4 cut(s) 81, 239, 1017, 1567
BseDI CCNNGG 7 cut(s) 43, 59, 659, 1016, 1565, 1747, 1748
BseGI GGATG 8 cut(s) 76, 165, 247, 466, 1453, 1477, 1771, 1870
BseJI GATNNNNATC 1 cut(s) 549
BseMII CTCAG 1 cut(s) 578
BseNI ACTGG 3 cut(s) 329, 475, 894
BseRI GAGGAG 3 cut(s) 308, 1344, 1826
BseX3I CGGCCG 1 cut(s) 40
BseXI GCAGC 2 cut(s) 76, 1041
Bsh1236I CGCG 2 cut(s) 45, 1407
Bsh1285I CGRYCG 1 cut(s) 43
BshFI GGCC 2 cut(s) 42, 1838
BshNI GGYRCC 2 cut(s) 998, 1591
BsiEI CGRYCG 1 cut(s) 43
BsiHKCI CYCGRG 1 cut(s) 1748
BsiSI CCGG 2 cut(s) 59, 1749
BslFI GGGAC 3 cut(s) 152, 966, 1799
BsmAI GTCTC 3 cut(s) 228, 412, 1356
BsmFI GGGAC 3 cut(s) 152, 966, 1799
BsnI GGCC 2 cut(s) 42, 1838
Bso31I GGTCTC 1 cut(s) 228
BsoBI CYCGRG 1 cut(s) 1748
Bsp1286I GDGCHC 1 cut(s) 1566
Bsp143I GATC 3 cut(s) 808, 1207, 1666
BspACI CCGC 9 cut(s) 39, 43, 45, 48, 137, 700, 812, 1027, 1808
BspANI GGCC 2 cut(s) 42, 1838
BspCNI CTCAG 1 cut(s) 577
BspFNI CGCG 2 cut(s) 45, 1407
BspHI TCATGA 3 cut(s) 961, 1141, 1314
BspLI GGNNCC 5 cut(s) 168, 1000, 1563, 1593, 1668
BspPI GGATC 3 cut(s) 803, 1661, 1674
BspT107I GGYRCC 2 cut(s) 998, 1591
BspTNI GGTCTC 1 cut(s) 228
BsrI ACTGG 3 cut(s) 329, 475, 894
BssECI CCNNGG 7 cut(s) 43, 59, 659, 1016, 1565, 1747, 1748
BssMI GATC 3 cut(s) 808, 1207, 1666
BssNI GRCGYC 2 cut(s) 9, 438
BssT1I CCWWGG 1 cut(s) 659
Bst2UI CCWGG 4 cut(s) 81, 239, 1017, 1567
Bst4CI ACNGT 1 cut(s) 837
Bst6I CTCTTC 5 cut(s) 23, 1359, 1362, 1437, 1768
BstACI GRCGYC 2 cut(s) 9, 438
BstC8I GCNNGC 2 cut(s) 714, 1551
BstDEI CTNAG 4 cut(s) 445, 564, 768, 1321
BstDSI CCRYGG 1 cut(s) 43
BstF5I GGATG 8 cut(s) 76, 165, 247, 466, 1453, 1477, 1771, 1870
BstFNI CGCG 2 cut(s) 45, 1407
BstKTI GATC 3 cut(s) 811, 1210, 1669
BstMAI GTCTC 3 cut(s) 228, 412, 1356
BstMBI GATC 3 cut(s) 808, 1207, 1666
BstMCI CGRYCG 1 cut(s) 43
BstMWI GCNNNNNNNGC 2 cut(s) 45, 48
BstNI CCWGG 4 cut(s) 81, 239, 1017, 1567
BstSCI CCNGG 7 cut(s) 58, 79, 237, 1015, 1565, 1747, 1748
BstUI CGCG 2 cut(s) 45, 1407
BstV1I GCAGC 2 cut(s) 76, 1041
BstV2I GAAGAC 2 cut(s) 1050, 1080
BstX2I RGATCY 1 cut(s) 1666
BstXI CCANNNNNNTGG 1 cut(s) 1912
BstYI RGATCY 1 cut(s) 1666
BstZI CGGCCG 1 cut(s) 40
BsuI GTATCC 1 cut(s) 849
BsuRI GGCC 2 cut(s) 42, 1838
BtgI CCRYGG 1 cut(s) 43
BtgZI GCGATG 1 cut(s) 1703
BtsCI GGATG 8 cut(s) 76, 165, 247, 466, 1453, 1477, 1771, 1870
BtsI GCAGTG 1 cut(s) 748
BtsIMutI CAGTG 5 cut(s) 319, 468, 748, 833, 1061
Cac8I GCNNGC 2 cut(s) 714, 1551
CciI TCATGA 3 cut(s) 961, 1141, 1314
CciNI GCGGCCGC 1 cut(s) 40
Cfr13I GGNCC 3 cut(s) 166, 1634, 1837
Cfr42I CCGCGG 1 cut(s) 46
Cfr9I CCCGGG 1 cut(s) 1748
Csp6I GTAC 3 cut(s) 211, 736, 922
CviAII CATG 9 cut(s) 156, 455, 962, 986, 1142, 1175, 1315, 1702, 1906
CviQI GTAC 3 cut(s) 211, 736, 922
DdeI CTNAG 4 cut(s) 445, 564, 768, 1321
DpnI GATC 3 cut(s) 810, 1209, 1668
DpnII GATC 3 cut(s) 808, 1207, 1666
DraI TTTAAA 2 cut(s) 912, 1299
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
EaeI YGGCCR 1 cut(s) 40
EagI CGGCCG 1 cut(s) 40
Eam1104I CTCTTC 5 cut(s) 23, 1359, 1362, 1437, 1768
EarI CTCTTC 5 cut(s) 23, 1359, 1362, 1437, 1768
EciI GGCGGA 1 cut(s) 801
EclXI CGGCCG 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 659
Eco24I GRGCYC 1 cut(s) 1566
Eco31I GGTCTC 1 cut(s) 228
Eco47I GGWCC 2 cut(s) 166, 1634
Eco52I CGGCCG 1 cut(s) 40
Eco57I CTGAAG 2 cut(s) 407, 1092
Eco88I CYCGRG 1 cut(s) 1748
EcoO109I RGGNCCY 1 cut(s) 166
EcoRII CCWGG 4 cut(s) 79, 237, 1015, 1565
EcoT14I CCWWGG 1 cut(s) 659
EcoT22I ATGCAT 1 cut(s) 1200
EcoT38I GRGCYC 1 cut(s) 1566
ErhI CCWWGG 1 cut(s) 659
FaeI CATG 9 cut(s) 159, 458, 965, 989, 1145, 1178, 1318, 1705, 1909
FalI AAGNNNNNCTT 2 cut(s) 1091, 1123
FaqI GGGAC 3 cut(s) 152, 966, 1799
FatI CATG 9 cut(s) 155, 454, 961, 985, 1141, 1174, 1314, 1701, 1905
FauNDI CATATG 1 cut(s) 1825
Fnu4HI GCNGC 6 cut(s) 40, 43, 46, 49, 65, 1030
FokI GGATG 8 cut(s) 63, 172, 254, 473, 1460, 1484, 1778, 1877
FriOI GRGCYC 1 cut(s) 1566
Fsp4HI GCNGC 6 cut(s) 40, 43, 46, 49, 65, 1030
FspBI CTAG 1 cut(s) 1521
GluI GCNGC 6 cut(s) 40, 43, 46, 49, 65, 1030
GsuI CTGGAG 3 cut(s) 492, 630, 1203
HaeIII GGCC 2 cut(s) 42, 1838
HapII CCGG 2 cut(s) 59, 1749
Hin1I GRCGYC 2 cut(s) 9, 438
Hin1II CATG 9 cut(s) 159, 458, 965, 989, 1145, 1178, 1318, 1705, 1909
HincII GTYRAC 2 cut(s) 370, 1390
HindII GTYRAC 2 cut(s) 370, 1390
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 7 cut(s) 371, 488, 550, 1496, 1511, 1916, 1928
HpaII CCGG 2 cut(s) 59, 1749
HphI GGTGA 6 cut(s) 433, 554, 626, 964, 1436, 1517
Hpy166II GTNNAC 4 cut(s) 370, 887, 972, 1390
Hpy188I TCNGA 7 cut(s) 7, 195, 281, 1220, 1260, 1516, 1933
Hpy8I GTNNAC 4 cut(s) 370, 887, 972, 1390
Hpy99I CGWCG 1 cut(s) 440
HpyAV CCTTC 5 cut(s) 157, 773, 1462, 1587, 1657
HpyCH4III ACNGT 1 cut(s) 837
HpyCH4IV ACGT 2 cut(s) 9, 438
HpyCH4V TGCA 7 cut(s) 647, 706, 856, 985, 1198, 1676, 1757
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 48
HpyF3I CTNAG 4 cut(s) 445, 564, 768, 1321
HpySE526I ACGT 2 cut(s) 9, 438
Hsp92I GRCGYC 2 cut(s) 9, 438
Hsp92II CATG 9 cut(s) 159, 458, 965, 989, 1145, 1178, 1318, 1705, 1909
KspI CCGCGG 1 cut(s) 46
Kzo9I GATC 3 cut(s) 808, 1207, 1666
LmnI GCTCC 2 cut(s) 473, 1561
Lsp1109I GCAGC 2 cut(s) 76, 1041
LweI GCATC 4 cut(s) 610, 1318, 1369, 1507
MaeI CTAG 1 cut(s) 1521
MaeII ACGT 2 cut(s) 9, 438
MalI GATC 3 cut(s) 810, 1209, 1668
MboI GATC 3 cut(s) 808, 1207, 1666
MfeI CAATTG 1 cut(s) 120
MflI RGATCY 1 cut(s) 1666
MhlI GDGCHC 1 cut(s) 1566
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 3 cut(s) 127, 549, 1511
Mph1103I ATGCAT 1 cut(s) 1200
MseI TTAA 6 cut(s) 198, 254, 341, 911, 1224, 1298
MslI CAYNNNNRTG 2 cut(s) 459, 1910
MspA1I CMGCKG 3 cut(s) 45, 1032, 1808
MspI CCGG 2 cut(s) 59, 1749
MspR9I CCNGG 7 cut(s) 60, 81, 239, 1017, 1567, 1749, 1750
MunI CAATTG 1 cut(s) 120
MvaI CCWGG 4 cut(s) 81, 239, 1017, 1567
MvnI CGCG 2 cut(s) 45, 1407
MwoI GCNNNNNNNGC 2 cut(s) 45, 48
NciI CCSGG 3 cut(s) 60, 1749, 1750
NdeI CATATG 1 cut(s) 1825
NdeII GATC 3 cut(s) 808, 1207, 1666
NlaIII CATG 9 cut(s) 159, 458, 965, 989, 1145, 1178, 1318, 1705, 1909
NlaIV GGNNCC 5 cut(s) 168, 1000, 1563, 1593, 1668
NmeAIII GCCGAG 1 cut(s) 15
NmuCI GTSAC 5 cut(s) 151, 244, 439, 575, 628
NotI GCGGCCGC 1 cut(s) 40
NsiI ATGCAT 1 cut(s) 1200
PagI TCATGA 3 cut(s) 961, 1141, 1314
PfeI GAWTC 6 cut(s) 488, 550, 1496, 1511, 1916, 1928
PfoI TCCNGGA 1 cut(s) 79
PkrI GCNGC 6 cut(s) 41, 44, 47, 50, 66, 1031
PleI GAGTC 1 cut(s) 365
PpsI GAGTC 1 cut(s) 365
PpuMI RGGWCCY 1 cut(s) 166
PsiI TTATAA 1 cut(s) 948
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 4 cut(s) 79, 237, 1015, 1565
PspGI CCWGG 4 cut(s) 79, 237, 1015, 1565
PspN4I GGNNCC 5 cut(s) 168, 1000, 1563, 1593, 1668
PspPI GGNCC 3 cut(s) 166, 1634, 1837
PspPPI RGGWCCY 1 cut(s) 166
PsuI RGATCY 1 cut(s) 1666
PvuII CAGCTG 1 cut(s) 1032
RsaI GTAC 3 cut(s) 212, 737, 923
RsaNI GTAC 3 cut(s) 211, 736, 922
RseI CAYNNNNRTG 2 cut(s) 459, 1910
SacII CCGCGG 1 cut(s) 46
SaqAI TTAA 6 cut(s) 198, 254, 341, 911, 1224, 1298
SatI GCNGC 6 cut(s) 40, 43, 46, 49, 65, 1030
Sau3AI GATC 3 cut(s) 808, 1207, 1666
Sau96I GGNCC 3 cut(s) 166, 1634, 1837
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 7 cut(s) 60, 81, 239, 1017, 1567, 1749, 1750
SduI GDGCHC 1 cut(s) 1566
SfaNI GCATC 4 cut(s) 610, 1318, 1369, 1507
Sfr303I CCGCGG 1 cut(s) 46
SgrBI CCGCGG 1 cut(s) 46
SinI GGWCC 2 cut(s) 166, 1634
SmaI CCCGGG 1 cut(s) 1750
SmiMI CAYNNNNRTG 2 cut(s) 459, 1910
SmlI CTYRAG 1 cut(s) 1332
SmoI CTYRAG 1 cut(s) 1332
SsiI CCGC 9 cut(s) 39, 43, 45, 48, 137, 700, 812, 1027, 1808
SspMI CTAG 1 cut(s) 1521
StyD4I CCNGG 7 cut(s) 58, 79, 237, 1015, 1565, 1747, 1748
StyI CCWWGG 1 cut(s) 659
TaaI ACNGT 1 cut(s) 837
TaiI ACGT 2 cut(s) 12, 441
TaqI TCGA 3 cut(s) 232, 590, 1623
TatI WGTACW 3 cut(s) 210, 735, 921
TauI GCSGC 4 cut(s) 42, 45, 48, 51
TfiI GAWTC 6 cut(s) 488, 550, 1496, 1511, 1916, 1928
Tru1I TTAA 6 cut(s) 198, 254, 341, 911, 1224, 1298
Tru9I TTAA 6 cut(s) 198, 254, 341, 911, 1224, 1298
TscAI CASTG 5 cut(s) 319, 475, 748, 840, 1068
TseFI GTSAC 5 cut(s) 151, 244, 439, 575, 628
TseI GCWGC 2 cut(s) 64, 1029
Tsp45I GTSAC 5 cut(s) 151, 244, 439, 575, 628
TspGWI ACGGA 3 cut(s) 809, 1092, 1778
TspMI CCCGGG 1 cut(s) 1748
TspRI CASTG 5 cut(s) 319, 475, 748, 840, 1068
VpaK11BI GGWCC 2 cut(s) 166, 1634
XapI RAATTY 3 cut(s) 218, 729, 1163
XmaI CCCGGG 1 cut(s) 1748
XspI CTAG 1 cut(s) 1521
ZraI GACGTC 2 cut(s) 10, 439
Zsp2I ATGCAT 1 cut(s) 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.