RchiOBHm_Chr3g0459221

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0010637
Physical Location & Seq
Reverse (-)
7701807 .. 7703356
1550 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42581

Sequence Viewer

Length: 759 bp
ATGATGAAATTCAAGGTTGACTTCACCGGCGCCGGCAGCTCAGGCGGAAAAGACGACGACAAGCCTATACTGACCTTGGGCACGGCCAAGCTCAAAGTCGACCAGGAAAACAAAACAGCCACCTTCATAGTCGACCAGTCCAAGCTCCCTGCTCGTCCCTACAGCTCCAAGCCCGAACCGATCACCGTGGGCCCGGAAGACCCTACGGACTTGCGACAAGTCTTCCAGAAGGTGGAGACCACCGAAGGCATCATCAACAATGTGGTCAAGATGTTCGACGCTCTACTCACAAAAGGCCACGAGCGCGAGGCTGCGGAGATGCATGCTCAGATCGACCAGGGTTTCATGCCCGAGGTGGTGACCTACACCGGCGTCATGGAGGAGTACGCCGGCGCCGGCAAGACAGGGGACGTTCTCAAGGTGTACCAGCGCATGTTGGACTACGGGATCAACCCTAACAGCTACACTTACAGGGTTCTCATCAAGGCGCTGGCGGAAGATCCGGACTCGAAGTTTCTCGGAGATGCGAAGAAGTACCTGATGGAAATGATGGACGTGTGTTTGCGGCCCGATGCGGTTGCGTACACGGCTGTGTTTAAGGCGTTTGCTCGGCTGGAGAAGGCGGCGGAGGGGAGGAGGTTTTTGGAGGAGATGAAGGGGAAGGGGTTTGTGGCGGACGAGAAGGAAGTGAGGGAGGTTCTCAAGGGCAGAAGAGGACGTTTGGCTAAGAAAGTCATCAATCTTCTATTTGGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

252

Amino Acids

28.12

Weight (kDa)

8.38

Isoelectric Point (pI)

23.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 112 - 164 7e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 117 - 165 2.1e-12 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 29, 392
AccB7I CCANNNNNTGG 1 cut(s) 232
AccI GTMKAC 2 cut(s) 99, 132
AccII CGCG 1 cut(s) 306
AccIII TCCGGA 1 cut(s) 502
AciI CCGC 8 cut(s) 45, 314, 494, 565, 575, 623, 626, 674
AclWI GGATC 2 cut(s) 455, 494
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 1 cut(s) 8
AcyI GRCGYC 3 cut(s) 30, 372, 393
AfaI GTAC 4 cut(s) 386, 425, 536, 584
AfiI CCNNNNNNNGG 1 cut(s) 232
AflIII ACRYGT 1 cut(s) 555
AgsI TTSAA 1 cut(s) 13
AjiI CACGTC 1 cut(s) 556
AjnI CCWGG 2 cut(s) 102, 336
AleI CACNNNNGTG 1 cut(s) 590
AluBI AGCT 5 cut(s) 39, 91, 145, 165, 462
AluI AGCT 5 cut(s) 39, 91, 145, 165, 462
Alw26I GTCTC 1 cut(s) 230
AlwI GGATC 2 cut(s) 455, 494
Ama87I CYCGRG 1 cut(s) 350
Aor13HI TCCGGA 1 cut(s) 502
AoxI GGCC 4 cut(s) 84, 190, 295, 566
ApaI GGGCCC 1 cut(s) 194
ApeKI GCWGC 2 cut(s) 36, 311
ApoI RAATTY 1 cut(s) 8
AspLEI GCGC 5 cut(s) 32, 306, 395, 432, 490
AspS9I GGNCC 3 cut(s) 190, 191, 567
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 3 cut(s) 16, 175, 370
AvaI CYCGRG 1 cut(s) 350
BaeGI GKGCMC 2 cut(s) 83, 194
BanI GGYRCC 2 cut(s) 29, 392
BanII GRGCYC 1 cut(s) 194
BauI CACGAG 1 cut(s) 299
BbsI GAAGAC 2 cut(s) 204, 214
BbvI GCAGC 2 cut(s) 48, 298
BccI CCATC 2 cut(s) 535, 544
BceAI ACGGC 2 cut(s) 99, 603
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciT130I CCWGG 2 cut(s) 104, 338
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 230
BfmI CTRYAG 1 cut(s) 160
BfoI RGCGCY 3 cut(s) 33, 396, 491
BisI GCNGC 4 cut(s) 37, 312, 566, 624
BlsI GCNGC 4 cut(s) 38, 313, 567, 625
Bme1390I CCNGG 3 cut(s) 104, 194, 338
BmeT110I CYCGRG 1 cut(s) 350
BmgBI CACGTC 1 cut(s) 556
BmgT120I GGNCC 3 cut(s) 190, 191, 567
BmiI GGNNCC 3 cut(s) 31, 192, 394
BmrFI CCNGG 3 cut(s) 104, 194, 338
BmsI GCATC 4 cut(s) 258, 309, 514, 562
BpiI GAAGAC 2 cut(s) 204, 214
BpmI CTGGAG 1 cut(s) 635
Bpu10I CCTNAGC 1 cut(s) 40
BpuEI CTTGAG 2 cut(s) 401, 686
BpuMI CCSGG 1 cut(s) 194
BsaHI GRCGYC 3 cut(s) 30, 372, 393
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 4 cut(s) 75, 186, 337, 351
BsaWI WCCGGW 1 cut(s) 502
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse118I RCCGGY 5 cut(s) 26, 32, 368, 389, 395
Bse1I ACTGG 1 cut(s) 136
BseAI TCCGGA 1 cut(s) 502
BseBI CCWGG 2 cut(s) 104, 338
BseDI CCNNGG 4 cut(s) 75, 186, 337, 351
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMII CTCAG 2 cut(s) 54, 341
BseNI ACTGG 1 cut(s) 136
BseRI GAGGAG 3 cut(s) 395, 649, 662
BseSI GKGCMC 2 cut(s) 83, 194
BseXI GCAGC 2 cut(s) 48, 298
Bsh1236I CGCG 1 cut(s) 306
BshFI GGCC 4 cut(s) 86, 192, 297, 568
BshNI GGYRCC 2 cut(s) 29, 392
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 7 cut(s) 27, 33, 194, 369, 390, 396, 503
BslFI GGGAC 2 cut(s) 141, 422
BslI CCNNNNNNNGG 1 cut(s) 232
BsmAI GTCTC 1 cut(s) 230
BsmFI GGGAC 2 cut(s) 141, 422
BsnI GGCC 4 cut(s) 86, 192, 297, 568
Bso31I GGTCTC 1 cut(s) 230
BsoBI CYCGRG 1 cut(s) 350
Bsp120I GGGCCC 1 cut(s) 190
Bsp1286I GDGCHC 2 cut(s) 83, 194
Bsp13I TCCGGA 1 cut(s) 502
Bsp143I GATC 4 cut(s) 180, 330, 447, 499
BspACI CCGC 8 cut(s) 45, 314, 494, 565, 575, 623, 626, 674
BspANI GGCC 4 cut(s) 86, 192, 297, 568
BspCNI CTCAG 2 cut(s) 53, 340
BspEI TCCGGA 1 cut(s) 502
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 3 cut(s) 31, 192, 394
BspPI GGATC 2 cut(s) 455, 494
BspT107I GGYRCC 2 cut(s) 29, 392
BspTNI GGTCTC 1 cut(s) 230
BsrFI RCCGGY 5 cut(s) 26, 32, 368, 389, 395
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 5 cut(s) 26, 32, 368, 389, 395
BssECI CCNNGG 4 cut(s) 75, 186, 337, 351
BssMI GATC 4 cut(s) 180, 330, 447, 499
BssNI GRCGYC 3 cut(s) 30, 372, 393
BssSI CACGAG 1 cut(s) 299
BssT1I CCWWGG 1 cut(s) 75
Bst2BI CACGAG 1 cut(s) 299
Bst2UI CCWGG 2 cut(s) 104, 338
Bst4CI ACNGT 1 cut(s) 187
Bst6I CTCTTC 1 cut(s) 706
BstACI GRCGYC 3 cut(s) 30, 372, 393
BstC8I GCNNGC 5 cut(s) 34, 324, 391, 397, 492
BstDEI CTNAG 3 cut(s) 40, 327, 726
BstDSI CCRYGG 1 cut(s) 186
BstEII GGTNACC 1 cut(s) 358
BstFNI CGCG 1 cut(s) 306
BstH2I RGCGCY 3 cut(s) 33, 396, 491
BstHHI GCGC 5 cut(s) 32, 306, 395, 432, 490
BstKTI GATC 4 cut(s) 183, 333, 450, 502
BstMAI GTCTC 1 cut(s) 230
BstMBI GATC 4 cut(s) 180, 330, 447, 499
BstMWI GCNNNNNNNGC 4 cut(s) 36, 42, 303, 587
BstNI CCWGG 2 cut(s) 104, 338
BstNSI RCATGY 2 cut(s) 326, 436
BstPI GGTNACC 1 cut(s) 358
BstSCI CCNGG 3 cut(s) 102, 192, 336
BstSFI CTRYAG 1 cut(s) 160
BstSLI GKGCMC 2 cut(s) 83, 194
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 2 cut(s) 48, 298
BstV2I GAAGAC 2 cut(s) 204, 214
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
BsuRI GGCC 4 cut(s) 86, 192, 297, 568
BtgI CCRYGG 1 cut(s) 186
BtrI CACGTC 1 cut(s) 556
Cac8I GCNNGC 5 cut(s) 34, 324, 391, 397, 492
CfoI GCGC 5 cut(s) 32, 306, 395, 432, 490
Cfr10I RCCGGY 5 cut(s) 26, 32, 368, 389, 395
Cfr13I GGNCC 3 cut(s) 190, 191, 567
CseI GACGC 2 cut(s) 287, 361
Csp6I GTAC 4 cut(s) 385, 424, 535, 583
CviAII CATG 4 cut(s) 323, 346, 376, 433
CviQI GTAC 4 cut(s) 385, 424, 535, 583
DdeI CTNAG 3 cut(s) 40, 327, 726
DinI GGCGCC 2 cut(s) 31, 394
DpnI GATC 4 cut(s) 182, 332, 449, 501
DpnII GATC 4 cut(s) 180, 330, 447, 499
EaeI YGGCCR 1 cut(s) 84
Eam1104I CTCTTC 1 cut(s) 706
EarI CTCTTC 1 cut(s) 706
EciI GGCGGA 4 cut(s) 60, 509, 641, 689
Eco130I CCWWGG 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 230
Eco88I CYCGRG 1 cut(s) 350
Eco91I GGTNACC 1 cut(s) 358
EcoO65I GGTNACC 1 cut(s) 358
EcoRII CCWGG 2 cut(s) 102, 336
EcoT14I CCWWGG 1 cut(s) 75
EcoT22I ATGCAT 1 cut(s) 324
EcoT38I GRGCYC 1 cut(s) 194
EgeI GGCGCC 2 cut(s) 31, 394
EheI GGCGCC 2 cut(s) 31, 394
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 4 cut(s) 326, 349, 379, 436
FaiI YATR 6 cut(s) 68, 128, 324, 347, 377, 434
FalI AAGNNNNNCTT 1 cut(s) 37
FaqI GGGAC 2 cut(s) 141, 422
FatI CATG 4 cut(s) 322, 345, 375, 432
FblI GTMKAC 2 cut(s) 99, 132
Fnu4HI GCNGC 4 cut(s) 37, 312, 566, 624
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 4 cut(s) 37, 312, 566, 624
GlaI GCGC 5 cut(s) 31, 305, 394, 431, 489
GluI GCNGC 4 cut(s) 37, 312, 566, 624
GsuI CTGGAG 1 cut(s) 635
HaeII RGCGCY 3 cut(s) 33, 396, 491
HaeIII GGCC 4 cut(s) 86, 192, 297, 568
HapII CCGG 7 cut(s) 27, 33, 194, 369, 390, 396, 503
HgaI GACGC 2 cut(s) 287, 361
HhaI GCGC 5 cut(s) 32, 306, 395, 432, 490
Hin1I GRCGYC 3 cut(s) 30, 372, 393
Hin1II CATG 4 cut(s) 326, 349, 379, 436
Hin6I GCGC 5 cut(s) 30, 304, 393, 430, 488
HinP1I GCGC 5 cut(s) 30, 304, 393, 430, 488
HincII GTYRAC 3 cut(s) 19, 100, 133
HindII GTYRAC 3 cut(s) 19, 100, 133
HinfI GANTC 1 cut(s) 506
HpaII CCGG 7 cut(s) 27, 33, 194, 369, 390, 396, 503
HphI GGTGA 3 cut(s) 16, 175, 370
Hpy166II GTNNAC 5 cut(s) 19, 100, 133, 424, 585
Hpy188I TCNGA 2 cut(s) 330, 521
Hpy188III TCNNGA 3 cut(s) 226, 268, 503
Hpy8I GTNNAC 5 cut(s) 19, 100, 133, 424, 585
Hpy99I CGWCG 2 cut(s) 59, 281
HpyAV CCTTC 7 cut(s) 133, 223, 239, 613, 649, 655, 676
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4IV ACGT 3 cut(s) 411, 555, 718
HpyCH4V TGCA 1 cut(s) 322
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 42, 303, 587
HpyF3I CTNAG 3 cut(s) 40, 327, 726
HpySE526I ACGT 3 cut(s) 411, 555, 718
Hsp92I GRCGYC 3 cut(s) 30, 372, 393
Hsp92II CATG 4 cut(s) 326, 349, 379, 436
HspAI GCGC 5 cut(s) 30, 304, 393, 430, 488
KasI GGCGCC 2 cut(s) 29, 392
Kpn2I TCCGGA 1 cut(s) 502
KroI GCCGGC 3 cut(s) 32, 389, 395
KroNI GCCGGC 3 cut(s) 34, 391, 397
Kzo9I GATC 4 cut(s) 180, 330, 447, 499
LmnI GCTCC 2 cut(s) 150, 170
Lsp1109I GCAGC 2 cut(s) 48, 298
LweI GCATC 4 cut(s) 258, 309, 514, 562
MaeII ACGT 3 cut(s) 411, 555, 718
MaeIII GTNAC 1 cut(s) 358
MalI GATC 4 cut(s) 182, 332, 449, 501
MboI GATC 4 cut(s) 180, 330, 447, 499
MboII GAAGA 6 cut(s) 209, 214, 509, 541, 723, 734
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 2 cut(s) 83, 194
MluCI AATT 1 cut(s) 8
Mly113I GGCGCC 2 cut(s) 30, 393
MlyI GAGTC 1 cut(s) 500
MmeI TCCRAC 1 cut(s) 417
Mph1103I ATGCAT 1 cut(s) 324
MreI CGCCGGCG 1 cut(s) 389
MroI TCCGGA 1 cut(s) 502
MroNI GCCGGC 3 cut(s) 32, 389, 395
MseI TTAA 1 cut(s) 597
MslI CAYNNNNRTG 1 cut(s) 590
MspI CCGG 7 cut(s) 27, 33, 194, 369, 390, 396, 503
MspR9I CCNGG 3 cut(s) 104, 194, 338
MvaI CCWGG 2 cut(s) 104, 338
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 4 cut(s) 36, 42, 303, 587
NaeI GCCGGC 3 cut(s) 34, 391, 397
NarI GGCGCC 2 cut(s) 30, 393
NciI CCSGG 1 cut(s) 194
NdeII GATC 4 cut(s) 180, 330, 447, 499
NgoMIV GCCGGC 3 cut(s) 32, 389, 395
NlaIII CATG 4 cut(s) 326, 349, 379, 436
NlaIV GGNNCC 3 cut(s) 31, 192, 394
NmeAIII GCCGAG 1 cut(s) 589
NmuCI GTSAC 1 cut(s) 358
NsiI ATGCAT 1 cut(s) 324
NspI RCATGY 2 cut(s) 326, 436
OliI CACNNNNGTG 1 cut(s) 590
PaeI GCATGC 1 cut(s) 326
PdiI GCCGGC 3 cut(s) 34, 391, 397
PflMI CCANNNNNTGG 1 cut(s) 232
PkrI GCNGC 4 cut(s) 38, 313, 567, 625
PleI GAGTC 1 cut(s) 500
PluTI GGCGCC 2 cut(s) 33, 396
PpsI GAGTC 1 cut(s) 500
Psp6I CCWGG 2 cut(s) 102, 336
PspEI GGTNACC 1 cut(s) 358
PspGI CCWGG 2 cut(s) 102, 336
PspN4I GGNNCC 3 cut(s) 31, 192, 394
PspOMI GGGCCC 1 cut(s) 190
PspPI GGNCC 3 cut(s) 190, 191, 567
PsuI RGATCY 1 cut(s) 499
RsaI GTAC 4 cut(s) 386, 425, 536, 584
RsaNI GTAC 4 cut(s) 385, 424, 535, 583
RseI CAYNNNNRTG 1 cut(s) 590
SalI GTCGAC 2 cut(s) 98, 131
SaqAI TTAA 1 cut(s) 597
SatI GCNGC 4 cut(s) 37, 312, 566, 624
Sau3AI GATC 4 cut(s) 180, 330, 447, 499
Sau96I GGNCC 3 cut(s) 190, 191, 567
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 3 cut(s) 104, 194, 338
SduI GDGCHC 2 cut(s) 83, 194
SfaNI GCATC 4 cut(s) 258, 309, 514, 562
SfcI CTRYAG 1 cut(s) 160
SfoI GGCGCC 2 cut(s) 31, 394
SgrAI CRCCGGYG 3 cut(s) 26, 368, 389
SmiMI CAYNNNNRTG 1 cut(s) 590
SmlI CTYRAG 2 cut(s) 416, 701
SmoI CTYRAG 2 cut(s) 416, 701
SphI GCATGC 1 cut(s) 326
Sse9I AATT 1 cut(s) 8
SsiI CCGC 8 cut(s) 45, 314, 494, 565, 575, 623, 626, 674
SspDI GGCGCC 2 cut(s) 29, 392
StyD4I CCNGG 3 cut(s) 102, 192, 336
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 1 cut(s) 187
TaiI ACGT 3 cut(s) 414, 558, 721
TaqI TCGA 5 cut(s) 99, 132, 276, 333, 509
TasI AATT 1 cut(s) 8
TauI GCSGC 2 cut(s) 568, 626
Tru1I TTAA 1 cut(s) 597
Tru9I TTAA 1 cut(s) 597
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 2 cut(s) 36, 311
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 4 cut(s) 20, 115, 334, 668
TspGWI ACGGA 1 cut(s) 221
Van91I CCANNNNNTGG 1 cut(s) 232
XapI RAATTY 1 cut(s) 8
XceI RCATGY 2 cut(s) 326, 436
XmiI GTMKAC 2 cut(s) 99, 132
Zsp2I ATGCAT 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.