Rroxscaffold_6G00420460

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0010637
Physical Location & Seq
Forward (+)
41710626 .. 41711970
1345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00420460.1

Sequence Viewer

Length: 720 bp
ATGACAAGAAAAACCTCTAAATCCAAGAGCAAGACTAAGGCGAAGGCTAACACTTCCGAGGAGATAGAGAGTTTATTAGAGATTGACAATGTCTACGACCCGGTTCCTCCTCCTTACCAACAGGGCCCCGACCCCATTGTGGTCTGTCCAAATGACCCCAAGGACTTGGCAGAAGTCTACGAGAAGGTGAACTTCTATGCCGCCGGCGTTATCTTGAGTGTTGTGAAGATGGTTGAGGGTCTGTCGATGGATGGCTACAACCTTCAGGCTGCGGAGATTGGGGCTGAGGTCATGGACAAGGGTAACATGCCTGGGGTGGTGGTCTTCACCGGCATCATTGAGGAGTATGCGAAAATGGGCAGGACCTTGGATGCTCTCAAGGCATACAAGAGGATGTTGACCTCTAAGGTCACACCCAATTCTTACACTTACAGTATTCTAATTGAGACTCTAGCCGGAGACGCTGATCCGAAGTTCCTTGTTGTTGCTAAGAAGTACATGCTGGAGATGATGGGGATGGGAATGAGGCCCAATGCATTGACTTATACTGCGGTGTTTAAGGGGTTTGCGCAGCGGGGGAAGACAGAGGAGGCGAAGCAGTTCCTGGAGGACATGAAAGCCAAGGGGTTTGAGCCGGACGAGAAGGCTGTAAGGGAGGTTCTTATGGGAAGAAGGGGACCTATGGTTAGAAGCCTCTTCAACATCCTCTTTGGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

239

Amino Acids

26.52

Weight (kDa)

8.39

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 104 - 152 1.7e-11 PPR repeat family
PPR_long PF17177 115 - 212 2.2e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 177 - 214 9.5e-09 PPR repeat family
PPR PF01535 181 - 210 5.2e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 570
AccI GTMKAC 2 cut(s) 93, 177
AciI CCGC 4 cut(s) 201, 272, 551, 574
AclWI GGATC 1 cut(s) 461
AcuI CTGAAG 1 cut(s) 248
AfaI GTAC 1 cut(s) 497
AfiI CCNNNNNNNGG 1 cut(s) 139
AgsI TTSAA 1 cut(s) 700
AjnI CCWGG 2 cut(s) 310, 603
Alw26I GTCTC 2 cut(s) 440, 453
AlwI GGATC 1 cut(s) 461
AlwNI CAGNNNCTG 1 cut(s) 604
AoxI GGCC 2 cut(s) 124, 527
ApaI GGGCCC 1 cut(s) 128
ApeKI GCWGC 2 cut(s) 269, 571
Asp700I GAANNNNTTC 1 cut(s) 599
AspLEI GCGC 1 cut(s) 571
AspS9I GGNCC 5 cut(s) 124, 125, 363, 528, 677
AsuC2I CCSGG 1 cut(s) 101
AsuHPI GGTGA 2 cut(s) 199, 319
AvaII GGWCC 2 cut(s) 363, 677
BaeGI GKGCMC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 128
BbsI GAAGAC 2 cut(s) 316, 587
BbvCI CCTCAGC 1 cut(s) 285
BbvI GCAGC 2 cut(s) 256, 583
BccI CCATC 5 cut(s) 223, 241, 245, 505, 511
BciT130I CCWGG 2 cut(s) 312, 605
BcnI CCSGG 1 cut(s) 101
BcoDI GTCTC 2 cut(s) 440, 453
BfaI CTAG 1 cut(s) 452
BisI GCNGC 3 cut(s) 201, 270, 572
BlsI GCNGC 3 cut(s) 202, 271, 573
Bme1390I CCNGG 3 cut(s) 101, 312, 605
Bme18I GGWCC 2 cut(s) 363, 677
BmgT120I GGNCC 5 cut(s) 124, 125, 363, 528, 677
BmiI GGNNCC 4 cut(s) 105, 126, 127, 678
BmrFI CCNGG 3 cut(s) 101, 312, 605
BmsI GCATC 2 cut(s) 342, 361
BpiI GAAGAC 2 cut(s) 316, 587
BpmI CTGGAG 2 cut(s) 524, 626
Bpu10I CCTNAGC 1 cut(s) 285
BpuEI CTTGAG 2 cut(s) 235, 362
BpuMI CCSGG 1 cut(s) 101
BsaJI CCNNGG 5 cut(s) 57, 159, 311, 366, 621
Bsc4I CCNNNNNNNGG 1 cut(s) 139
Bse118I RCCGGY 2 cut(s) 203, 329
BseBI CCWGG 2 cut(s) 312, 605
BseDI CCNNGG 5 cut(s) 57, 159, 311, 366, 621
BseGI GGATG 5 cut(s) 256, 376, 399, 522, 702
BseLI CCNNNNNNNGG 1 cut(s) 139
BseMII CTCAG 1 cut(s) 276
BseRI GAGGAG 4 cut(s) 74, 99, 356, 602
BseSI GKGCMC 1 cut(s) 128
BseXI GCAGC 2 cut(s) 256, 583
BshFI GGCC 2 cut(s) 126, 529
BsiSI CCGG 5 cut(s) 101, 204, 330, 456, 635
BslFI GGGAC 1 cut(s) 690
BslI CCNNNNNNNGG 1 cut(s) 139
BsmAI GTCTC 2 cut(s) 440, 453
BsmBI CGTCTC 1 cut(s) 453
BsmFI GGGAC 1 cut(s) 690
BsnI GGCC 2 cut(s) 126, 529
Bsp120I GGGCCC 1 cut(s) 124
Bsp1286I GDGCHC 1 cut(s) 128
Bsp143I GATC 1 cut(s) 466
BspACI CCGC 4 cut(s) 201, 272, 551, 574
BspANI GGCC 2 cut(s) 126, 529
BspCNI CTCAG 1 cut(s) 277
BspLI GGNNCC 4 cut(s) 105, 126, 127, 678
BspPI GGATC 1 cut(s) 461
BsrFI RCCGGY 2 cut(s) 203, 329
BssAI RCCGGY 2 cut(s) 203, 329
BssECI CCNNGG 5 cut(s) 57, 159, 311, 366, 621
BssMI GATC 1 cut(s) 466
BssT1I CCWWGG 3 cut(s) 159, 366, 621
Bst2UI CCWGG 2 cut(s) 312, 605
Bst4CI ACNGT 1 cut(s) 434
Bst6I CTCTTC 1 cut(s) 701
BstC8I GCNNGC 1 cut(s) 205
BstDEI CTNAG 4 cut(s) 36, 285, 405, 489
BstF5I GGATG 5 cut(s) 256, 376, 399, 522, 702
BstHHI GCGC 1 cut(s) 571
BstKTI GATC 1 cut(s) 469
BstMAI GTCTC 2 cut(s) 440, 453
BstMBI GATC 1 cut(s) 466
BstMWI GCNNNNNNNGC 2 cut(s) 380, 461
BstNI CCWGG 2 cut(s) 312, 605
BstNSI RCATGY 2 cut(s) 310, 502
BstSCI CCNGG 3 cut(s) 99, 310, 603
BstSLI GKGCMC 1 cut(s) 128
BstV1I GCAGC 2 cut(s) 256, 583
BstV2I GAAGAC 2 cut(s) 316, 587
BstXI CCANNNNNNTGG 1 cut(s) 166
BsuRI GGCC 2 cut(s) 126, 529
BtsCI GGATG 5 cut(s) 256, 376, 399, 522, 702
Cac8I GCNNGC 1 cut(s) 205
CaiI CAGNNNCTG 1 cut(s) 604
CfoI GCGC 1 cut(s) 571
Cfr10I RCCGGY 2 cut(s) 203, 329
Cfr13I GGNCC 5 cut(s) 124, 125, 363, 528, 677
CseI GACGC 1 cut(s) 470
Csp6I GTAC 1 cut(s) 496
CviAII CATG 4 cut(s) 292, 307, 499, 613
CviQI GTAC 1 cut(s) 496
DdeI CTNAG 4 cut(s) 36, 285, 405, 489
DpnI GATC 1 cut(s) 468
DpnII GATC 1 cut(s) 466
Eam1104I CTCTTC 1 cut(s) 701
EarI CTCTTC 1 cut(s) 701
Eco130I CCWWGG 3 cut(s) 159, 366, 621
Eco24I GRGCYC 1 cut(s) 128
Eco47I GGWCC 2 cut(s) 363, 677
Eco57I CTGAAG 1 cut(s) 248
EcoO109I RGGNCCY 4 cut(s) 124, 125, 363, 677
EcoRII CCWGG 2 cut(s) 310, 603
EcoT14I CCWWGG 3 cut(s) 159, 366, 621
EcoT22I ATGCAT 1 cut(s) 538
EcoT38I GRGCYC 1 cut(s) 128
ErhI CCWWGG 3 cut(s) 159, 366, 621
Esp3I CGTCTC 1 cut(s) 453
FaeI CATG 4 cut(s) 295, 310, 502, 616
FalI AAGNNNNNCTT 2 cut(s) 176, 208
FaqI GGGAC 1 cut(s) 690
FatI CATG 4 cut(s) 291, 306, 498, 612
FauI CCCGC 1 cut(s) 567
FblI GTMKAC 2 cut(s) 93, 177
Fnu4HI GCNGC 3 cut(s) 201, 270, 572
FokI GGATG 5 cut(s) 263, 383, 406, 529, 689
FriOI GRGCYC 1 cut(s) 128
Fsp4HI GCNGC 3 cut(s) 201, 270, 572
FspBI CTAG 1 cut(s) 452
FspI TGCGCA 1 cut(s) 570
GlaI GCGC 1 cut(s) 570
GluI GCNGC 3 cut(s) 201, 270, 572
GsuI CTGGAG 2 cut(s) 524, 626
HaeIII GGCC 2 cut(s) 126, 529
HapII CCGG 5 cut(s) 101, 204, 330, 456, 635
HgaI GACGC 1 cut(s) 470
HhaI GCGC 1 cut(s) 571
Hin1II CATG 4 cut(s) 295, 310, 502, 616
Hin6I GCGC 1 cut(s) 569
HinP1I GCGC 1 cut(s) 569
HincII GTYRAC 1 cut(s) 399
HindII GTYRAC 1 cut(s) 399
HinfI GANTC 1 cut(s) 448
HpaII CCGG 5 cut(s) 101, 204, 330, 456, 635
HphI GGTGA 2 cut(s) 199, 319
Hpy166II GTNNAC 4 cut(s) 94, 178, 190, 399
Hpy188I TCNGA 2 cut(s) 58, 471
Hpy188III TCNNGA 1 cut(s) 214
Hpy8I GTNNAC 4 cut(s) 94, 178, 190, 399
HpyAV CCTTC 5 cut(s) 37, 178, 272, 637, 666
HpyCH4III ACNGT 1 cut(s) 434
HpyCH4V TGCA 1 cut(s) 536
HpyF10VI GCNNNNNNNGC 2 cut(s) 380, 461
HpyF3I CTNAG 4 cut(s) 36, 285, 405, 489
Hsp92II CATG 4 cut(s) 295, 310, 502, 616
HspAI GCGC 1 cut(s) 569
KroI GCCGGC 1 cut(s) 203
KroNI GCCGGC 1 cut(s) 205
Kzo9I GATC 1 cut(s) 466
Lsp1109I GCAGC 2 cut(s) 256, 583
LweI GCATC 2 cut(s) 342, 361
MaeI CTAG 1 cut(s) 452
MaeIII GTNAC 2 cut(s) 302, 409
MalI GATC 1 cut(s) 468
MboI GATC 1 cut(s) 466
MboII GAAGA 5 cut(s) 238, 316, 592, 681, 688
MhlI GDGCHC 1 cut(s) 128
MluCI AATT 2 cut(s) 418, 441
MlyI GAGTC 1 cut(s) 442
Mph1103I ATGCAT 1 cut(s) 538
MreI CGCCGGCG 1 cut(s) 203
MroNI GCCGGC 1 cut(s) 203
MroXI GAANNNNTTC 1 cut(s) 599
MseI TTAA 1 cut(s) 558
MspA1I CMGCKG 1 cut(s) 574
MspI CCGG 5 cut(s) 101, 204, 330, 456, 635
MspR9I CCNGG 3 cut(s) 101, 312, 605
MvaI CCWGG 2 cut(s) 312, 605
MwoI GCNNNNNNNGC 2 cut(s) 380, 461
NaeI GCCGGC 1 cut(s) 205
NciI CCSGG 1 cut(s) 101
NdeII GATC 1 cut(s) 466
NgoMIV GCCGGC 1 cut(s) 203
NlaIII CATG 4 cut(s) 295, 310, 502, 616
NlaIV GGNNCC 4 cut(s) 105, 126, 127, 678
NmuCI GTSAC 1 cut(s) 409
NsbI TGCGCA 1 cut(s) 570
NsiI ATGCAT 1 cut(s) 538
NspI RCATGY 2 cut(s) 310, 502
PdiI GCCGGC 1 cut(s) 205
PdmI GAANNNNTTC 1 cut(s) 599
PflFI GACNNNGTC 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 603
PkrI GCNGC 3 cut(s) 202, 271, 573
PleI GAGTC 1 cut(s) 442
PpsI GAGTC 1 cut(s) 442
PpuMI RGGWCCY 2 cut(s) 363, 677
Psp5II RGGWCCY 2 cut(s) 363, 677
Psp6I CCWGG 2 cut(s) 310, 603
PspGI CCWGG 2 cut(s) 310, 603
PspN4I GGNNCC 4 cut(s) 105, 126, 127, 678
PspOMI GGGCCC 1 cut(s) 124
PspPI GGNCC 5 cut(s) 124, 125, 363, 528, 677
PspPPI RGGWCCY 2 cut(s) 363, 677
PsrI GAACNNNNNNTAC 2 cut(s) 642, 674
PstNI CAGNNNCTG 1 cut(s) 604
PsyI GACNNNGTC 1 cut(s) 89
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
SaqAI TTAA 1 cut(s) 558
SatI GCNGC 3 cut(s) 201, 270, 572
Sau3AI GATC 1 cut(s) 466
Sau96I GGNCC 5 cut(s) 124, 125, 363, 528, 677
SchI GAGTC 1 cut(s) 442
ScrFI CCNGG 3 cut(s) 101, 312, 605
SduI GDGCHC 1 cut(s) 128
SetI ASST 9 cut(s) 17, 189, 264, 291, 368, 404, 411, 660, 682
SfaNI GCATC 2 cut(s) 342, 361
SgrAI CRCCGGYG 1 cut(s) 203
SinI GGWCC 2 cut(s) 363, 677
SmlI CTYRAG 2 cut(s) 214, 377
SmoI CTYRAG 2 cut(s) 214, 377
Sse9I AATT 2 cut(s) 418, 441
SsiI CCGC 4 cut(s) 201, 272, 551, 574
SspMI CTAG 1 cut(s) 452
StyD4I CCNGG 3 cut(s) 99, 310, 603
StyI CCWWGG 3 cut(s) 159, 366, 621
TaaI ACNGT 1 cut(s) 434
TaqI TCGA 1 cut(s) 245
TasI AATT 2 cut(s) 418, 441
TatI WGTACW 1 cut(s) 495
TauI GCSGC 1 cut(s) 203
Tru1I TTAA 1 cut(s) 558
Tru9I TTAA 1 cut(s) 558
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 2 cut(s) 269, 571
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 1 cut(s) 629
Tth111I GACNNNGTC 1 cut(s) 89
VpaK11BI GGWCC 2 cut(s) 363, 677
XceI RCATGY 2 cut(s) 310, 502
XmiI GTMKAC 2 cut(s) 93, 177
XmnI GAANNNNTTC 1 cut(s) 599
XspI CTAG 1 cut(s) 452
Zsp2I ATGCAT 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.