RchiOBHm_Chr3g0492421
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0014715
Physical Location & Seq
Forward (+)
39176552 .. 39177808
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45531

Sequence Viewer

Length: 957 bp
ATGGAGAAGAAGAAACCCCAACATGTTGGGTACTACTACAAGCTCTTGGCTTTTCTGGCTGCTGGGTTTTCTTCCCTGTTGCTCTGTGCCAACTCTCAACCAAGCGCAAGCAACGATGCTGCAGTAATGCTAGCCCTCAAGAAGAGCCTCAGCCCGTCTAGTTCATTCGGATGGTCCGACCCGGACCCGTGCAAGTGGAGCCATGTAGGCTGCTCAGGTGACAATAGGGTGACCCGGATCCAGATTGGTCACCAAAACCTTCAAGGTACGCTCCCTACAAGCCTCCAAAACCTCACTCAGCTACAGCGATTAGAGGTTCAGTGGAACAAGATTTCGGGGCCTCTTCCAAGCCTAAGTGGGTTGGGCTCGTTACAAGTCTTGTTACTTGGTAACAACTTGTTTACTTCAATTCCTAGTGATTTCTTTGCTGGCATGACTTCACTTCAGTCTGTTGAGATTGACAACAACCCATTTACGGCTTGGGAAATCCCCCAAACCCTCCAGAACGCATCGTCACTTCAAAATTTCTCAGCCAATTCCGCCAACATTACCGGAAAGATACCGGGTTTTTTCAACGCCGATGCGTTTCCCGGGCTGGTTAACTTGCACTTGGCTTTCAATTACCTGGAGGGCCAATTGCCGGAGAGCTTTTCGGGGTCAGAAATGCAGTCTCTTTGGCTTAATGGGCAGCAAAGTGTAGGTAAGCTTGTTGGCAGCATTGATGTTTTACGCAACATGACTACGCTGACTGAGGTTTGGTTGCATTCAAATGGGTTTTCAGGCCCGTTGCCCGACTTTTCGGGGTTGACAGATTTGAGGAGCTTAAGTGTAAGAGATAATTTGTTCACCGGACCTGTTCCGGTGTCATTGTTGAACCTCAAGTCATTAGAGTCTCTGAATTTGACAAACAACTTGCTCCAAGGACCAATGCCAGATTTTCCCAAGGGGTTTCAGTAG

Protein Analysis

318

Amino Acids

34.48

Weight (kDa)

5.93

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 72 3.2e-07 Leucine rich repeat N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 540
AclWI GGATC 2 cut(s) 232, 245
AcsI RAATTY 2 cut(s) 523, 898
AcuI CTGAAG 1 cut(s) 428
AfaI GTAC 2 cut(s) 32, 268
AfiI CCNNNNNNNGG 2 cut(s) 475, 640
AflII CTTAAG 1 cut(s) 823
AflIII ACRYGT 1 cut(s) 22
AgsI TTSAA 7 cut(s) 263, 408, 521, 574, 619, 768, 874
AjnI CCWGG 1 cut(s) 624
AluBI AGCT 5 cut(s) 43, 301, 648, 706, 822
AluI AGCT 5 cut(s) 43, 301, 648, 706, 822
Alw26I GTCTC 2 cut(s) 675, 897
AlwI GGATC 2 cut(s) 232, 245
Ama87I CYCGRG 1 cut(s) 590
AoxI GGCC 3 cut(s) 338, 631, 781
ApeKI GCWGC 5 cut(s) 59, 119, 210, 688, 714
ApoI RAATTY 2 cut(s) 523, 898
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 7 cut(s) 174, 184, 338, 631, 782, 851, 923
AsuC2I CCSGG 5 cut(s) 182, 235, 564, 591, 592
AsuHPI GGTGA 4 cut(s) 230, 241, 242, 838
AsuNHI GCTAGC 1 cut(s) 130
AvaI CYCGRG 1 cut(s) 590
AvaII GGWCC 4 cut(s) 174, 184, 851, 923
BamHI GGATCC 1 cut(s) 237
BanII GRGCYC 1 cut(s) 368
BbvCI CCTCAGC 1 cut(s) 149
BbvI GCAGC 5 cut(s) 46, 106, 197, 700, 726
BccI CCATC 1 cut(s) 165
BceAI ACGGC 1 cut(s) 492
BciT130I CCWGG 1 cut(s) 626
BcnI CCSGG 5 cut(s) 182, 235, 564, 591, 592
BcoDI GTCTC 2 cut(s) 675, 897
BfaI CTAG 3 cut(s) 131, 159, 414
BfmI CTRYAG 2 cut(s) 120, 302
BfrI CTTAAG 1 cut(s) 823
BglI GCCNNNNNGGC 1 cut(s) 207
BisI GCNGC 5 cut(s) 60, 120, 211, 689, 715
BlsI GCNGC 5 cut(s) 61, 121, 212, 690, 716
Bme1390I CCNGG 6 cut(s) 182, 235, 564, 591, 592, 626
Bme18I GGWCC 4 cut(s) 174, 184, 851, 923
BmeT110I CYCGRG 1 cut(s) 590
BmgT120I GGNCC 7 cut(s) 174, 184, 338, 631, 782, 851, 923
BmiI GGNNCC 4 cut(s) 186, 200, 239, 339
BmrFI CCNGG 6 cut(s) 182, 235, 564, 591, 592, 626
BmsI GCATC 3 cut(s) 106, 518, 571
BmtI GCTAGC 1 cut(s) 134
BpmI CTGGAG 2 cut(s) 485, 647
Bpu10I CCTNAGC 2 cut(s) 149, 214
BpuEI CTTGAG 2 cut(s) 122, 863
BpuMI CCSGG 5 cut(s) 182, 235, 564, 591, 592
BsaJI CCNNGG 3 cut(s) 590, 919, 942
BsaWI WCCGGW 3 cut(s) 551, 848, 859
BsaXI ACNNNNNCTCC 2 cut(s) 811, 841
Bsc4I CCNNNNNNNGG 2 cut(s) 475, 640
BseBI CCWGG 1 cut(s) 626
BseDI CCNNGG 3 cut(s) 590, 919, 942
BseGI GGATG 1 cut(s) 176
BseLI CCNNNNNNNGG 2 cut(s) 475, 640
BseMII CTCAG 5 cut(s) 163, 228, 311, 543, 741
BseRI GAGGAG 1 cut(s) 832
BseXI GCAGC 5 cut(s) 46, 106, 197, 700, 726
BseYI CCCAGC 1 cut(s) 62
BshFI GGCC 3 cut(s) 340, 633, 783
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 8 cut(s) 182, 235, 552, 563, 591, 641, 849, 860
BslI CCNNNNNNNGG 2 cut(s) 475, 640
BsmAI GTCTC 2 cut(s) 675, 897
BsmI GAATGC 1 cut(s) 763
BsnI GGCC 3 cut(s) 340, 633, 783
BsoBI CYCGRG 1 cut(s) 590
Bsp1286I GDGCHC 1 cut(s) 368
Bsp143I GATC 1 cut(s) 237
BspACI CCGC 1 cut(s) 540
BspANI GGCC 3 cut(s) 340, 633, 783
BspCNI CTCAG 5 cut(s) 162, 227, 310, 542, 742
BspLI GGNNCC 4 cut(s) 186, 200, 239, 339
BspMAI CTGCAG 1 cut(s) 124
BspOI GCTAGC 1 cut(s) 134
BspPI GGATC 2 cut(s) 232, 245
BspQI GCTCTTC 1 cut(s) 137
BspTI CTTAAG 1 cut(s) 823
BssECI CCNNGG 3 cut(s) 590, 919, 942
BssMI GATC 1 cut(s) 237
BssT1I CCWWGG 2 cut(s) 919, 942
Bst2UI CCWGG 1 cut(s) 626
Bst6I CTCTTC 2 cut(s) 137, 348
BstAFI CTTAAG 1 cut(s) 823
BstC8I GCNNGC 3 cut(s) 109, 132, 430
BstDEI CTNAG 6 cut(s) 149, 214, 297, 353, 529, 750
BstEII GGTNACC 2 cut(s) 229, 248
BstF5I GGATG 1 cut(s) 176
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 1 cut(s) 240
BstMAI GTCTC 2 cut(s) 675, 897
BstMBI GATC 1 cut(s) 237
BstMWI GCNNNNNNNGC 5 cut(s) 56, 198, 207, 539, 685
BstNI CCWGG 1 cut(s) 626
BstNSI RCATGY 1 cut(s) 26
BstPI GGTNACC 2 cut(s) 229, 248
BstSCI CCNGG 6 cut(s) 180, 233, 562, 589, 590, 624
BstSFI CTRYAG 2 cut(s) 120, 302
BstV1I GCAGC 5 cut(s) 46, 106, 197, 700, 726
BstX2I RGATCY 1 cut(s) 237
BstXI CCANNNNNNTGG 1 cut(s) 26
BstYI RGATCY 1 cut(s) 237
BsuRI GGCC 3 cut(s) 340, 633, 783
BtsCI GGATG 1 cut(s) 176
BtsIMutI CAGTG 1 cut(s) 326
Cac8I GCNNGC 3 cut(s) 109, 132, 430
CfoI GCGC 1 cut(s) 107
Cfr13I GGNCC 7 cut(s) 174, 184, 338, 631, 782, 851, 923
Cfr9I CCCGGG 1 cut(s) 590
Csp6I GTAC 2 cut(s) 31, 267
CviAII CATG 4 cut(s) 23, 203, 433, 736
CviQI GTAC 2 cut(s) 31, 267
DdeI CTNAG 6 cut(s) 149, 214, 297, 353, 529, 750
DpnI GATC 1 cut(s) 239
DpnII GATC 1 cut(s) 237
Eam1104I CTCTTC 2 cut(s) 137, 348
EarI CTCTTC 2 cut(s) 137, 348
EciI GGCGGA 1 cut(s) 529
Eco130I CCWWGG 2 cut(s) 919, 942
Eco24I GRGCYC 1 cut(s) 368
Eco47I GGWCC 4 cut(s) 174, 184, 851, 923
Eco57I CTGAAG 1 cut(s) 428
Eco88I CYCGRG 1 cut(s) 590
Eco91I GGTNACC 2 cut(s) 229, 248
EcoO109I RGGNCCY 1 cut(s) 338
EcoO65I GGTNACC 2 cut(s) 229, 248
EcoRII CCWGG 1 cut(s) 624
EcoT14I CCWWGG 2 cut(s) 919, 942
EcoT38I GRGCYC 1 cut(s) 368
ErhI CCWWGG 2 cut(s) 919, 942
FaeI CATG 4 cut(s) 26, 206, 436, 739
FaiI YATR 4 cut(s) 24, 204, 434, 737
FatI CATG 4 cut(s) 22, 202, 432, 735
Fnu4HI GCNGC 5 cut(s) 60, 120, 211, 689, 715
FokI GGATG 1 cut(s) 183
FriOI GRGCYC 1 cut(s) 368
Fsp4HI GCNGC 5 cut(s) 60, 120, 211, 689, 715
FspBI CTAG 3 cut(s) 131, 159, 414
GlaI GCGC 1 cut(s) 106
GluI GCNGC 5 cut(s) 60, 120, 211, 689, 715
GsaI CCCAGC 1 cut(s) 66
GsuI CTGGAG 2 cut(s) 485, 647
HaeIII GGCC 3 cut(s) 340, 633, 783
HapII CCGG 8 cut(s) 182, 235, 552, 563, 591, 641, 849, 860
HhaI GCGC 1 cut(s) 107
Hin1II CATG 4 cut(s) 26, 206, 436, 739
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HincII GTYRAC 2 cut(s) 601, 807
HindII GTYRAC 2 cut(s) 601, 807
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 1 cut(s) 890
HpaI GTTAAC 1 cut(s) 601
HpaII CCGG 8 cut(s) 182, 235, 552, 563, 591, 641, 849, 860
HphI GGTGA 4 cut(s) 230, 241, 242, 838
Hpy166II GTNNAC 4 cut(s) 402, 601, 807, 846
Hpy188I TCNGA 4 cut(s) 170, 178, 661, 897
Hpy188III TCNNGA 3 cut(s) 139, 241, 502
Hpy8I GTNNAC 4 cut(s) 402, 601, 807, 846
HpyAV CCTTC 1 cut(s) 269
HpyCH4V TGCA 5 cut(s) 122, 192, 607, 667, 763
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 198, 207, 539, 685
HpyF3I CTNAG 6 cut(s) 149, 214, 297, 353, 529, 750
Hsp92II CATG 4 cut(s) 26, 206, 436, 739
HspAI GCGC 1 cut(s) 105
KspAI GTTAAC 1 cut(s) 601
Kzo9I GATC 1 cut(s) 237
LguI GCTCTTC 1 cut(s) 137
LmnI GCTCC 4 cut(s) 198, 276, 819, 921
Lsp1109I GCAGC 5 cut(s) 46, 106, 197, 700, 726
LweI GCATC 3 cut(s) 106, 518, 571
MaeI CTAG 3 cut(s) 131, 159, 414
MaeIII GTNAC 7 cut(s) 218, 229, 248, 369, 381, 389, 513
MalI GATC 1 cut(s) 239
MboI GATC 1 cut(s) 237
MboII GAAGA 5 cut(s) 19, 22, 63, 154, 335
MfeI CAATTG 1 cut(s) 635
MflI RGATCY 1 cut(s) 237
MhlI GDGCHC 1 cut(s) 368
MluCI AATT 7 cut(s) 408, 523, 535, 619, 635, 838, 898
MlyI GAGTC 1 cut(s) 899
MmeI TCCRAC 1 cut(s) 201
MseI TTAA 3 cut(s) 600, 681, 824
MslI CAYNNNNRTG 2 cut(s) 169, 768
MspCI CTTAAG 1 cut(s) 823
MspI CCGG 8 cut(s) 182, 235, 552, 563, 591, 641, 849, 860
MspR9I CCNGG 6 cut(s) 182, 235, 564, 591, 592, 626
MunI CAATTG 1 cut(s) 635
Mva1269I GAATGC 1 cut(s) 763
MvaI CCWGG 1 cut(s) 626
MwoI GCNNNNNNNGC 5 cut(s) 56, 198, 207, 539, 685
NciI CCSGG 5 cut(s) 182, 235, 564, 591, 592
NdeII GATC 1 cut(s) 237
NheI GCTAGC 1 cut(s) 130
NlaIII CATG 4 cut(s) 26, 206, 436, 739
NlaIV GGNNCC 4 cut(s) 186, 200, 239, 339
NmuCI GTSAC 4 cut(s) 218, 229, 248, 513
NspI RCATGY 1 cut(s) 26
PciI ACATGT 1 cut(s) 22
PciSI GCTCTTC 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PctI GAATGC 1 cut(s) 763
PkrI GCNGC 5 cut(s) 61, 121, 212, 690, 716
PleI GAGTC 1 cut(s) 898
PpsI GAGTC 1 cut(s) 898
PscI ACATGT 1 cut(s) 22
Psp6I CCWGG 1 cut(s) 624
PspEI GGTNACC 2 cut(s) 229, 248
PspFI CCCAGC 1 cut(s) 62
PspGI CCWGG 1 cut(s) 624
PspN4I GGNNCC 4 cut(s) 186, 200, 239, 339
PspPI GGNCC 7 cut(s) 174, 184, 338, 631, 782, 851, 923
PstI CTGCAG 1 cut(s) 124
PsuI RGATCY 1 cut(s) 237
RsaI GTAC 2 cut(s) 32, 268
RsaNI GTAC 2 cut(s) 31, 267
RseI CAYNNNNRTG 2 cut(s) 169, 768
SapI GCTCTTC 1 cut(s) 137
SaqAI TTAA 3 cut(s) 600, 681, 824
SatI GCNGC 5 cut(s) 60, 120, 211, 689, 715
Sau3AI GATC 1 cut(s) 237
Sau96I GGNCC 7 cut(s) 174, 184, 338, 631, 782, 851, 923
SchI GAGTC 1 cut(s) 899
ScrFI CCNGG 6 cut(s) 182, 235, 564, 591, 592, 626
SduI GDGCHC 1 cut(s) 368
SfaNI GCATC 3 cut(s) 106, 518, 571
SfcI CTRYAG 2 cut(s) 120, 302
SinI GGWCC 4 cut(s) 174, 184, 851, 923
SmaI CCCGGG 1 cut(s) 592
SmiMI CAYNNNNRTG 2 cut(s) 169, 768
SmlI CTYRAG 3 cut(s) 137, 823, 878
SmoI CTYRAG 3 cut(s) 137, 823, 878
Sse9I AATT 7 cut(s) 408, 523, 535, 619, 635, 838, 898
SsiI CCGC 1 cut(s) 540
SspMI CTAG 3 cut(s) 131, 159, 414
StyD4I CCNGG 6 cut(s) 180, 233, 562, 589, 590, 624
StyI CCWWGG 2 cut(s) 919, 942
TasI AATT 7 cut(s) 408, 523, 535, 619, 635, 838, 898
Tru1I TTAA 3 cut(s) 600, 681, 824
Tru9I TTAA 3 cut(s) 600, 681, 824
TscAI CASTG 1 cut(s) 326
TseFI GTSAC 4 cut(s) 218, 229, 248, 513
TseI GCWGC 5 cut(s) 59, 119, 210, 688, 714
Tsp45I GTSAC 4 cut(s) 218, 229, 248, 513
TspDTI ATGAA 1 cut(s) 153
TspMI CCCGGG 1 cut(s) 590
TspRI CASTG 1 cut(s) 326
Vha464I CTTAAG 1 cut(s) 823
VpaK11BI GGWCC 4 cut(s) 174, 184, 851, 923
XapI RAATTY 2 cut(s) 523, 898
XceI RCATGY 1 cut(s) 26
XcmI CCANNNNNNNNNTGG 1 cut(s) 477
XmaI CCCGGG 1 cut(s) 590
XspI CTAG 3 cut(s) 131, 159, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.