RchiOBHm_Chr4g0390341

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0017810
Physical Location & Seq
Reverse (-)
5673490 .. 5678394
4905 bp
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UTR
Exon/CDS
Intron
PRQ36337

Sequence Viewer

Length: 579 bp
ATGAAGGTGGCAGTGATTGGTTCTGGTATTAGTGGTTTGGTCTCGGCCTATGTTCTTGCAAAAGAAGGTGTTGAAGTGGTTCTATATGAGAAAAAAGACTACCTGGGTGGCCATGCCAAGACTGTCACATATGATGGTGTTGATTTGGACCTTGGTCTCATGGTCTTCAATCATGTGATGTATCTAAATATGATGGAGTTCTTTGAGAGCCTTGGAGTTGATATGGAGACATGCGACATGTCTTTCTCAATGAGTTTAGATAAAGGACGGGGCTGTGAATGGGGTAGTCTAAATGGTCTCTCAAGCTTGTTTGCAGAAAAGAAGAACTTGTTCAACCCATACTTCTGGAAAATGCTTCAAGAAATCACCAAGTTTAAGCATGATTTGATCAGTTATCTTGAGGTGCTTGAGAGTAACCTAGACATTCACCGGAATGAAACCTTGGGGCAGTTTATTGAGTCACATCACTACTCTGAACTATTTCTGAAGGCTTACCTTATTCCAATATGTAGTTCAATCTTGTCGTGTCCTTCAGAAGGAGTTATAAGCTTCTCAGCTTTCTCAGTGCTTTCGGAATGA

Protein Analysis

192

Amino Acids

21.56

Weight (kDa)

4.92

Isoelectric Point (pI)

33.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 2 - 39 5.4e-08 FAD dependent oxidoreductase
FAD_binding_2 PF00890 3 - 39 2.5e-06 FAD binding domain
FAD_oxidored PF12831 3 - 68 2.4e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 5 - 70 5.9e-17 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 10 - 77 7.4e-12 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 545
AcoI YGGCCR 1 cut(s) 109
AcuI CTGAAG 2 cut(s) 506, 516
AfiI CCNNNNNNNGG 1 cut(s) 536
AflIII ACRYGT 1 cut(s) 237
AgsI TTSAA 5 cut(s) 74, 169, 334, 359, 516
AjnI CCWGG 1 cut(s) 102
AjuI GAANNNNNNNTTGG 4 cut(s) 425, 457, 496, 528
AluBI AGCT 3 cut(s) 306, 549, 557
AluI AGCT 3 cut(s) 306, 549, 557
Alw26I GTCTC 4 cut(s) 46, 161, 221, 302
AoxI GGCC 2 cut(s) 45, 109
Asp700I GAANNNNTTC 3 cut(s) 78, 329, 480
AspS9I GGNCC 1 cut(s) 148
AsuHPI GGTGA 2 cut(s) 358, 419
AvaII GGWCC 1 cut(s) 148
BalI TGGCCA 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 157
BccI CCATC 2 cut(s) 128, 187
BciT130I CCWGG 1 cut(s) 104
BclI TGATCA 1 cut(s) 387
BcoDI GTCTC 4 cut(s) 46, 161, 221, 302
BfaI CTAG 1 cut(s) 419
Bme1390I CCNGG 1 cut(s) 104
Bme18I GGWCC 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 148
BmrFI CCNGG 1 cut(s) 104
BoxI GACNNNNGTC 1 cut(s) 153
BpiI GAAGAC 1 cut(s) 157
BpuEI CTTGAG 3 cut(s) 286, 419, 428
BsaI GGTCTC 3 cut(s) 46, 161, 302
BsaJI CCNNGG 4 cut(s) 103, 151, 211, 441
BsaWI WCCGGW 1 cut(s) 429
Bsc4I CCNNNNNNNGG 1 cut(s) 536
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 4 cut(s) 103, 151, 211, 441
BseLI CCNNNNNNNGG 1 cut(s) 536
BseMII CTCAG 2 cut(s) 567, 576
BshFI GGCC 2 cut(s) 47, 111
BsiSI CCGG 1 cut(s) 430
BslI CCNNNNNNNGG 1 cut(s) 536
BsmAI GTCTC 4 cut(s) 46, 161, 221, 302
BsnI GGCC 2 cut(s) 47, 111
Bso31I GGTCTC 3 cut(s) 46, 161, 302
Bsp143I GATC 1 cut(s) 387
BspANI GGCC 2 cut(s) 47, 111
BspCNI CTCAG 2 cut(s) 566, 575
BspTNI GGTCTC 3 cut(s) 46, 161, 302
BssECI CCNNGG 4 cut(s) 103, 151, 211, 441
BssMI GATC 1 cut(s) 387
BssT1I CCWWGG 3 cut(s) 151, 211, 441
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 1 cut(s) 124
BstDEI CTNAG 2 cut(s) 553, 562
BstENI CCTNNNNNAGG 1 cut(s) 534
BstKTI GATC 1 cut(s) 390
BstMAI GTCTC 4 cut(s) 46, 161, 221, 302
BstMBI GATC 1 cut(s) 387
BstNI CCWGG 1 cut(s) 104
BstNSI RCATGY 2 cut(s) 234, 241
BstPAI GACNNNNGTC 1 cut(s) 153
BstSCI CCNGG 1 cut(s) 102
BstV2I GAAGAC 1 cut(s) 157
BstXI CCANNNNNNTGG 1 cut(s) 345
BsuRI GGCC 2 cut(s) 47, 111
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 2 cut(s) 18, 570
Cfr13I GGNCC 1 cut(s) 148
CviAII CATG 6 cut(s) 113, 160, 173, 231, 238, 380
CviJI RGCY 8 cut(s) 47, 111, 210, 273, 306, 491, 549, 557
CviKI_1 RGCY 8 cut(s) 47, 111, 210, 273, 306, 491, 549, 557
DdeI CTNAG 2 cut(s) 553, 562
DpnI GATC 1 cut(s) 389
DpnII GATC 1 cut(s) 387
EaeI YGGCCR 1 cut(s) 109
Eco130I CCWWGG 3 cut(s) 151, 211, 441
Eco31I GGTCTC 3 cut(s) 46, 161, 302
Eco47I GGWCC 1 cut(s) 148
Eco57I CTGAAG 2 cut(s) 506, 516
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoRII CCWGG 1 cut(s) 102
EcoT14I CCWWGG 3 cut(s) 151, 211, 441
ErhI CCWWGG 3 cut(s) 151, 211, 441
FaeI CATG 6 cut(s) 116, 163, 176, 234, 241, 383
FalI AAGNNNNNCTT 2 cut(s) 311, 343
FatI CATG 6 cut(s) 112, 159, 172, 230, 237, 379
FauNDI CATATG 1 cut(s) 130
FbaI TGATCA 1 cut(s) 387
FspBI CTAG 1 cut(s) 419
HaeIII GGCC 2 cut(s) 47, 111
HapII CCGG 1 cut(s) 430
Hin1II CATG 6 cut(s) 116, 163, 176, 234, 241, 383
HindIII AAGCTT 2 cut(s) 304, 547
HinfI GANTC 1 cut(s) 458
HpaII CCGG 1 cut(s) 430
HphI GGTGA 2 cut(s) 358, 419
Hpy188I TCNGA 4 cut(s) 475, 486, 535, 574
Hpy188III TCNNGA 3 cut(s) 346, 359, 398
HpyAV CCTTC 4 cut(s) 59, 481, 530, 540
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4V TGCA 2 cut(s) 59, 314
HpyF3I CTNAG 2 cut(s) 553, 562
Hsp92II CATG 6 cut(s) 116, 163, 176, 234, 241, 383
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 1 cut(s) 387
LpnPI CCDG 5 cut(s) 9, 89, 116, 331, 443
MaeI CTAG 1 cut(s) 419
MaeIII GTNAC 3 cut(s) 124, 413, 459
MalI GATC 1 cut(s) 389
MboI GATC 1 cut(s) 387
MboII GAAGA 2 cut(s) 157, 334
MlsI TGGCCA 1 cut(s) 111
MluNI TGGCCA 1 cut(s) 111
MlyI GAGTC 1 cut(s) 467
MnlI CCTC 1 cut(s) 394
Mox20I TGGCCA 1 cut(s) 111
MroXI GAANNNNTTC 3 cut(s) 78, 329, 480
MscI TGGCCA 1 cut(s) 111
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 1 cut(s) 432
Msp20I TGGCCA 1 cut(s) 111
MspI CCGG 1 cut(s) 430
MspR9I CCNGG 1 cut(s) 104
MvaI CCWGG 1 cut(s) 104
NdeI CATATG 1 cut(s) 130
NdeII GATC 1 cut(s) 387
NlaIII CATG 6 cut(s) 116, 163, 176, 234, 241, 383
NmeAIII GCCGAG 1 cut(s) 23
NmuCI GTSAC 2 cut(s) 124, 459
NspI RCATGY 2 cut(s) 234, 241
PciI ACATGT 1 cut(s) 237
PdmI GAANNNNTTC 3 cut(s) 78, 329, 480
PleI GAGTC 1 cut(s) 466
PpsI GAGTC 1 cut(s) 466
PscI ACATGT 1 cut(s) 237
PshAI GACNNNNGTC 1 cut(s) 153
PsiI TTATAA 1 cut(s) 545
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PspPI GGNCC 1 cut(s) 148
RseI CAYNNNNRTG 1 cut(s) 432
SaqAI TTAA 1 cut(s) 375
Sau3AI GATC 1 cut(s) 387
Sau96I GGNCC 1 cut(s) 148
SchI GAGTC 1 cut(s) 467
ScrFI CCNGG 1 cut(s) 104
SinI GGWCC 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 3 cut(s) 301, 398, 407
SmoI CTYRAG 3 cut(s) 301, 398, 407
SspMI CTAG 1 cut(s) 419
StyD4I CCNGG 1 cut(s) 102
StyI CCWWGG 3 cut(s) 151, 211, 441
TaaI ACNGT 1 cut(s) 124
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 2 cut(s) 18, 570
TseFI GTSAC 2 cut(s) 124, 459
Tsp45I GTSAC 2 cut(s) 124, 459
TspDTI ATGAA 2 cut(s) 17, 450
TspRI CASTG 2 cut(s) 18, 570
VpaK11BI GGWCC 1 cut(s) 148
XagI CCTNNNNNAGG 1 cut(s) 534
XceI RCATGY 2 cut(s) 234, 241
XmnI GAANNNNTTC 3 cut(s) 78, 329, 480
XspI CTAG 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.