Rroxscaffold_7G00183690

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0017810
Physical Location & Seq
Reverse (-)
22771398 .. 22789764
18367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00183690.1

Sequence Viewer

Length: 333 bp
ATGAAAGTGGCTTCGATTGGTTCTGGTATTAGTGGTTTGGTCTCAGCTTATTTTCTTGCAAAAGAAGTGGTTCTATATGAGAAAAAAGTCTACCCGGGTGATCATGCCGGGATTGTCACATATGATGGTGTTGATTTGGACCTTGGTTTCATGGTCTTTAATCATGTAATGTATCCAAATATGATGGAGTTCTTTGAGAGCCTTGGAGTTGATATGGAGACATGCAACATGTCCTTCTCAGTGAGTTTAGATAAAGGACGGGGATGTGAATGGGGTAGTCGAAATAGTCTCTCAAGCTTGTTTGCAGAAAACAAGAACTTGTCCAACCCATAA

Protein Analysis

110

Amino Acids

12.09

Weight (kDa)

4.8

Isoelectric Point (pI)

15.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 6 - 68 1.5e-10 NAD(P)-binding Rossmann-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 90
AflIII ACRYGT 1 cut(s) 228
AluBI AGCT 2 cut(s) 47, 297
AluI AGCT 2 cut(s) 47, 297
Alw26I GTCTC 3 cut(s) 46, 212, 293
Ama87I CYCGRG 1 cut(s) 94
Asp700I GAANNNNTTC 1 cut(s) 69
AspS9I GGNCC 1 cut(s) 139
AsuC2I CCSGG 3 cut(s) 95, 96, 109
AsuHPI GGTGA 1 cut(s) 110
AvaI CYCGRG 1 cut(s) 94
AvaII GGWCC 1 cut(s) 139
BccI CCATC 2 cut(s) 119, 178
BciVI GTATCC 1 cut(s) 183
BclI TGATCA 1 cut(s) 100
BcnI CCSGG 3 cut(s) 95, 96, 109
BcoDI GTCTC 3 cut(s) 46, 212, 293
BfuI GTATCC 1 cut(s) 183
Bme1390I CCNGG 3 cut(s) 95, 96, 109
Bme18I GGWCC 1 cut(s) 139
BmeT110I CYCGRG 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 139
BmrFI CCNGG 3 cut(s) 95, 96, 109
BpuEI CTTGAG 1 cut(s) 277
BpuMI CCSGG 3 cut(s) 95, 96, 109
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 3 cut(s) 94, 142, 202
BseDI CCNNGG 3 cut(s) 94, 142, 202
BseGI GGATG 1 cut(s) 269
BseMII CTCAG 2 cut(s) 57, 252
BsiHKCI CYCGRG 1 cut(s) 94
BsiSI CCGG 2 cut(s) 95, 108
BsmAI GTCTC 3 cut(s) 46, 212, 293
Bso31I GGTCTC 1 cut(s) 46
BsoBI CYCGRG 1 cut(s) 94
Bsp143I GATC 1 cut(s) 100
BspCNI CTCAG 2 cut(s) 56, 251
BspTNI GGTCTC 1 cut(s) 46
BssECI CCNNGG 3 cut(s) 94, 142, 202
BssMI GATC 1 cut(s) 100
BssT1I CCWWGG 2 cut(s) 142, 202
BstDEI CTNAG 2 cut(s) 43, 238
BstF5I GGATG 1 cut(s) 269
BstKTI GATC 1 cut(s) 103
BstMAI GTCTC 3 cut(s) 46, 212, 293
BstMBI GATC 1 cut(s) 100
BstNSI RCATGY 2 cut(s) 225, 232
BstSCI CCNGG 3 cut(s) 93, 94, 107
BsuI GTATCC 1 cut(s) 183
BtsCI GGATG 1 cut(s) 269
BtsIMutI CAGTG 1 cut(s) 246
Cfr13I GGNCC 1 cut(s) 139
Cfr9I CCCGGG 1 cut(s) 94
CspCI CAANNNNNGTGG 2 cut(s) 48, 83
CviAII CATG 5 cut(s) 104, 151, 164, 222, 229
CviJI RGCY 4 cut(s) 11, 47, 201, 297
CviKI_1 RGCY 4 cut(s) 11, 47, 201, 297
DdeI CTNAG 2 cut(s) 43, 238
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 142, 202
Eco31I GGTCTC 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 139
Eco88I CYCGRG 1 cut(s) 94
EcoT14I CCWWGG 2 cut(s) 142, 202
ErhI CCWWGG 2 cut(s) 142, 202
FaeI CATG 5 cut(s) 107, 154, 167, 225, 232
FatI CATG 5 cut(s) 103, 150, 163, 221, 228
FauNDI CATATG 1 cut(s) 121
FbaI TGATCA 1 cut(s) 100
FblI GTMKAC 1 cut(s) 90
FokI GGATG 1 cut(s) 276
HapII CCGG 2 cut(s) 95, 108
Hin1II CATG 5 cut(s) 107, 154, 167, 225, 232
HindIII AAGCTT 1 cut(s) 295
HpaII CCGG 2 cut(s) 95, 108
HphI GGTGA 1 cut(s) 110
Hpy166II GTNNAC 1 cut(s) 91
Hpy8I GTNNAC 1 cut(s) 91
HpyAV CCTTC 1 cut(s) 244
HpyCH4V TGCA 3 cut(s) 59, 225, 305
HpyF3I CTNAG 2 cut(s) 43, 238
Hsp92II CATG 5 cut(s) 107, 154, 167, 225, 232
Ksp22I TGATCA 1 cut(s) 100
Kzo9I GATC 1 cut(s) 100
LpnPI CCDG 3 cut(s) 9, 108, 121
MaeIII GTNAC 1 cut(s) 115
MalI GATC 1 cut(s) 102
MboI GATC 1 cut(s) 100
MroXI GAANNNNTTC 1 cut(s) 69
MseI TTAA 1 cut(s) 159
MspI CCGG 2 cut(s) 95, 108
MspR9I CCNGG 3 cut(s) 95, 96, 109
NciI CCSGG 3 cut(s) 95, 96, 109
NdeI CATATG 1 cut(s) 121
NdeII GATC 1 cut(s) 100
NlaIII CATG 5 cut(s) 107, 154, 167, 225, 232
NmuCI GTSAC 1 cut(s) 115
NspI RCATGY 2 cut(s) 225, 232
PciI ACATGT 1 cut(s) 228
PdmI GAANNNNTTC 1 cut(s) 69
PscI ACATGT 1 cut(s) 228
PspPI GGNCC 1 cut(s) 139
SaqAI TTAA 1 cut(s) 159
Sau3AI GATC 1 cut(s) 100
Sau96I GGNCC 1 cut(s) 139
ScrFI CCNGG 3 cut(s) 95, 96, 109
SetI ASST 3 cut(s) 49, 144, 299
SinI GGWCC 1 cut(s) 139
SmaI CCCGGG 1 cut(s) 96
SmlI CTYRAG 1 cut(s) 292
SmoI CTYRAG 1 cut(s) 292
StyD4I CCNGG 3 cut(s) 93, 94, 107
StyI CCWWGG 2 cut(s) 142, 202
TaqI TCGA 2 cut(s) 14, 280
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 1 cut(s) 246
TseFI GTSAC 1 cut(s) 115
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 2 cut(s) 17, 139
TspMI CCCGGG 1 cut(s) 94
TspRI CASTG 1 cut(s) 246
VpaK11BI GGWCC 1 cut(s) 139
XceI RCATGY 2 cut(s) 225, 232
XmaI CCCGGG 1 cut(s) 94
XmiI GTMKAC 1 cut(s) 90
XmnI GAANNNNTTC 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.