RchiOBHm_Chr4g0424901

Cell division control protein 24, OB domain 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0014037
Physical Location & Seq
Forward (+)
50468131 .. 50472679
4549 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39414

Sequence Viewer

Length: 2136 bp
ATGTACGGGCCGGCCCAGCACATATTTCAAAACCCGCGCCAAAACGCCGAACGAGTTGCCCTTTCCCTCCCATTATATAACCCCGAGTTTAGTTCAGTAGGGTTTGCTGTGAACTCGGTGGAAATGAACTCACTCGACTCGCCGGACCGCAATCCGCAACCACAAGAAGAAGACCCACTCCTCAAATTCATCGATTACGCCAGGTCCGCTCTCTCCCCTGAAATCGACGACGATTCGGATCCCAACGGAAATGGGACGGAGACCGCCCCGCCCAGCTGGAATTGGATCGCCTCTCGGATTCTCAAGACCTGCAGCGCCTACTCCAGCGGCGTTACGGCCGCGATTTTGCTCTCCGATCTCTCACAGGCATGGCATGAGCAAAATAGGGACAGGCCTGTGAAGAAAATGCCGGAATGCGTGAAGCAATTGCGAAAGAAGAATAAGCGAAGGAAGCTTTCGAACACTGTGACTATTGATTCCATTTACGAGAAGAATTTCTTGTCCTTGAATAGTGTGTTGGAAGCAGTGATTGTCAACGTTTTTGTTCTCCCTGGCACCAACATTTATATGCTTTCCCTGGGGGACTTTTGGAGCACCAATACCATTGATCTTTATCTCCACCGCAGGTACTATGACTTGATAAATCCTACTAATGGGATTCTCAAGAAAGGAAGGCAGATCTTCCTCACGGGGTGCCATCTTCGTGCTGCTGCTGGAGGAGGGTCTCGTTGTCCTCGACTGTTGCCAACAGAATATCTAGTGATATTGTTGGATGAGGATGATGATGGTGATGCAATGCTGCTAGGAGCTCAGTTTTGTTCAGATTCATTCTCCTCAATTTCTCTTGATGCATTTAGTAAAAACGTTTCTTATTCATTGTATGCAAGAATTGAGCTCATTGGCCCCCTGGAAGTTCGAGGAAAGTTTGGAACTTTACAAAGGAAACAAATCACTCTTGTAGATGATGATGGTGCCAAGTTGCAATTTCTGCTGTGGGGTGACCAGGTTGTCCTTGCAAATCTTTTCAGTGTGGGAAGTATGCTTGCACTGGATAAACCATATATTGCAAGTTCTGCAGACAGTGCTGCTGAAACAAGTGCTGAAGTTTCCCTTGAATTTGGTAGTGCAACACAATTGTTTTTGGTGCCTTTTGTTCAACACAAGGAACAAGTATGTGTATCACTGACACCAAGCCGCCATCAAGGATCAAGACTGCTAAGCACATTAGATCCCAGTCAGAGTACACAAGTTTCTCAAGTTTCCTTGCCCTGTGATTCACAAGGGTCTATTGATTTTAGTAGTTATCCTTTCCAATCCTACATTACCGATCTTCGTGACAAGATGACAGGCGTCAGCCTTTATGGTGTTGTTACTAGTATCATCAAAGAAAGATGTAACACAGAAGCTATCTTCTCATTGCGAATTGAAGATACCACTGGAACAATTTGGACTAAATTACATTTTGTGAAATATTGGTCACTAGGAAGATTAAGCCTTGGCCACACCGTCTACATATCTGGTCTGACATGCTCTATGAAAAAGCGGAAGGGGTTCCAGCTAAACAGATGCCATGACGGTCTAGAAGCAATATGGTCCGAGAATACTGGTGGAGCTTCTTTCTTCAACCTTAGTAGCTTGCCAGCGCTGCTAAACTCATCTTGTCTTCATAAACTAACAAAGCTCTCTGATCTGTCCAGCCAGACTAGTTGTACTCAGATAGTTCATGTTCAGCTTGAAATGGTTCAATTTCATCTCAGTACAAGGTTGTCACATGCTGTATGTGGTCATTTTGTTACCGCGCAACCCATGGGAGTTTCTAAATGCAGATTCTGTAATGAGGATTGTGATGAAGTAATTCGCACTTTCCACCTGAAAATGACTCTTGCTGACAAGAGTGGGAAAGTTTTTGCATGGTGTACTGGTCATACTGCTACAGAGATCCTGCAAATATCTCCTGATGAATTCTTTGAACTACCTGAGGAAGAACAAGTTATGTATCCCGCTTCATTAGATAATGAGAGGTTCATGGTTGCAATTGTTAACTGCAAAAGGCAAGGTTCTGGACCTGGTGATGCTCTGGTGCTAGCAACTGAGGGAATCTCATGGGAGATTACTCGTGCACTGAAGTGCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDC24_OB1 CDC24_OB2 CDC24_OB3

Protein Analysis

711

Amino Acids

78.95

Weight (kDa)

5.79

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC24_OB1 PF17246 61 - 187 1.2e-45 Cell division control protein 24, OB domain 1
CDC24_OB2 PF17245 193 - 322 3.9e-46 Cell division control protein 24, OB domain 2
CDC24_OB3 PF17244 443 - 670 6.7e-77 Cell division control protein 24, OB domain 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17030
fragaria_vesca FvH4_4g19640
malus_domestica MD13G1155000.v1.1
prunus_persica Prupe.1G118000_v2.0.a1
pyrus_communis pycom13g13180
rosa_chinensis RchiOBHm_Chr4g0424901 RchiOBHm_Chr4g0424931
rosa_laevigata RLG00000007411
rosa_multiflora Rmu_sc0006754.1_g000002 Rmu_ssc0000420.1_g000005
rosa_roxburghii Rroxscaffold_5G00367120
rosa_rugosa Rorug04G0200500
rosa_samantha Rh4AG257900 Rh4BG263300 Rh4CG274300 Rh4DG259000
rosa_wichuraiana Rw4G022260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1007
Acc36I ACCTGC 2 cut(s) 317, 615
AccB1I GGYRCC 4 cut(s) 554, 693, 971, 1144
AccBSI CCGCTC 1 cut(s) 209
AccI GTMKAC 1 cut(s) 1509
AccII CGCG 3 cut(s) 37, 341, 1799
AclI AACGTT 2 cut(s) 537, 864
AclWI GGATC 6 cut(s) 233, 246, 293, 1213, 1223, 1933
AcoI YGGCCR 2 cut(s) 336, 1498
AcsI RAATTY 4 cut(s) 185, 493, 1115, 1961
AcuI CTGAAG 1 cut(s) 1122
AcyI GRCGYC 1 cut(s) 1350
AdeI CACNNNGTG 1 cut(s) 693
AfaI GTAC 6 cut(s) 5, 629, 1243, 1711, 1759, 1918
AfeI AGCGCT 1 cut(s) 1644
AfiI CCNNNNNNNGG 1 cut(s) 653
AgsI TTSAA 9 cut(s) 29, 508, 1115, 1157, 1427, 1624, 1736, 1745, 1970
AhlI ACTAGT 2 cut(s) 1373, 1703
AjnI CCWGG 6 cut(s) 200, 550, 576, 906, 1002, 2065
AjuI GAANNNNNNNTTGG 2 cut(s) 500, 532
AleI CACNNNNGTG 1 cut(s) 2125
Alw21I GWGCWC 4 cut(s) 596, 811, 897, 2122
Alw26I GTCTC 2 cut(s) 254, 729
Alw44I GTGCAC 1 cut(s) 2118
AlwI GGATC 6 cut(s) 233, 246, 293, 1213, 1223, 1933
AlwNI CAGNNNCTG 1 cut(s) 1830
Ama87I CYCGRG 1 cut(s) 83
Aor51HI AGCGCT 1 cut(s) 1644
AoxI GGCC 6 cut(s) 8, 12, 336, 392, 901, 1498
ApaLI GTGCAC 1 cut(s) 2118
ApeKI GCWGC 6 cut(s) 312, 707, 710, 799, 1085, 1645
ApoI RAATTY 4 cut(s) 185, 493, 1115, 1961
Asp700I GAANNNNTTC 4 cut(s) 494, 1550, 1740, 1854
AspLEI GCGC 4 cut(s) 39, 317, 1645, 1801
AspS9I GGNCC 7 cut(s) 8, 13, 145, 204, 902, 1593, 2063
AsuHPI GGTGA 3 cut(s) 800, 1010, 2081
AsuII TTCGAA 1 cut(s) 458
AsuNHI GCTAGC 1 cut(s) 2083
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 4 cut(s) 145, 204, 1593, 2063
AxyI CCTNAGG 1 cut(s) 1977
BaeGI GKGCMC 1 cut(s) 2122
BalI TGGCCA 1 cut(s) 1500
BamHI GGATCC 1 cut(s) 238
BanI GGYRCC 4 cut(s) 554, 693, 971, 1144
BanII GRGCYC 2 cut(s) 811, 897
BauI CACGAG 1 cut(s) 2115
BbsI GAAGAC 2 cut(s) 177, 1655
Bbv12I GWGCWC 4 cut(s) 596, 811, 897, 2122
BbvI GCAGC 6 cut(s) 324, 694, 697, 786, 1072, 1632
BccI CCATC 4 cut(s) 705, 779, 962, 1206
BceAI ACGGC 1 cut(s) 351
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciT130I CCWGG 6 cut(s) 202, 552, 578, 908, 1004, 2067
BciVI GTATCC 1 cut(s) 2007
BcoDI GTCTC 2 cut(s) 254, 729
BcuI ACTAGT 2 cut(s) 1373, 1703
BfaI CTAG 7 cut(s) 758, 803, 1374, 1481, 1580, 1704, 2084
BfmI CTRYAG 3 cut(s) 310, 1074, 1932
BfoI RGCGCY 2 cut(s) 318, 1646
BfuAI ACCTGC 2 cut(s) 317, 615
BfuI GTATCC 1 cut(s) 2007
BglII AGATCT 1 cut(s) 678
BisI GCNGC 9 cut(s) 313, 328, 339, 708, 711, 800, 1086, 1195, 1646
BlpI GCTNAGC 1 cut(s) 1217
BlsI GCNGC 9 cut(s) 314, 329, 340, 709, 712, 801, 1087, 1196, 1647
Bme1390I CCNGG 6 cut(s) 202, 552, 578, 908, 1004, 2067
Bme18I GGWCC 4 cut(s) 145, 204, 1593, 2063
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 7 cut(s) 8, 13, 145, 204, 902, 1593, 2063
BmiI GGNNCC 7 cut(s) 240, 556, 695, 904, 973, 1146, 1553
BmrFI CCNGG 6 cut(s) 202, 552, 578, 908, 1004, 2067
BmrI ACTGGG 1 cut(s) 1227
BmsI GCATC 4 cut(s) 781, 838, 1556, 2062
BmtI GCTAGC 1 cut(s) 2087
BmuI ACTGGG 1 cut(s) 1227
BoxI GACNNNNGTC 1 cut(s) 1349
BpiI GAAGAC 2 cut(s) 177, 1655
BplI GAGNNNNNCTC 2 cut(s) 2084, 2116
BpmI CTGGAG 2 cut(s) 307, 735
Bpu1102I GCTNAGC 1 cut(s) 1217
Bpu14I TTCGAA 1 cut(s) 458
BpuEI CTTGAG 3 cut(s) 287, 647, 1239
Bsa29I ATCGAT 1 cut(s) 192
BsaBI GATNNNNATC 2 cut(s) 237, 612
BsaHI GRCGYC 1 cut(s) 1350
BsaI GGTCTC 2 cut(s) 254, 729
BsaJI CCNNGG 6 cut(s) 550, 576, 577, 906, 1495, 1806
Bsc4I CCNNNNNNNGG 1 cut(s) 653
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 5 cut(s) 1053, 1233, 1441, 1609, 1924
Bse21I CCTNAGG 1 cut(s) 1977
Bse3DI GCAATG 2 cut(s) 801, 1415
Bse8I GATNNNNATC 2 cut(s) 237, 612
BseBI CCWGG 6 cut(s) 202, 552, 578, 908, 1004, 2067
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 6 cut(s) 550, 576, 577, 906, 1495, 1806
BseGI GGATG 2 cut(s) 778, 784
BseJI GATNNNNATC 2 cut(s) 237, 612
BseLI CCNNNNNNNGG 1 cut(s) 653
BseMI GCAATG 2 cut(s) 801, 1415
BseMII CTCAG 5 cut(s) 824, 1727, 1768, 1968, 2082
BseNI ACTGG 5 cut(s) 1053, 1233, 1441, 1609, 1924
BseRI GAGGAG 3 cut(s) 170, 732, 823
BseSI GKGCMC 1 cut(s) 2122
BseX3I CGGCCG 1 cut(s) 336
BseXI GCAGC 6 cut(s) 324, 694, 697, 786, 1072, 1632
BseYI CCCAGC 2 cut(s) 15, 272
Bsh1236I CGCG 3 cut(s) 37, 341, 1799
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 6 cut(s) 10, 14, 338, 394, 903, 1500
BshNI GGYRCC 4 cut(s) 554, 693, 971, 1144
BshVI ATCGAT 1 cut(s) 192
BsiEI CGRYCG 1 cut(s) 339
BsiHKAI GWGCWC 4 cut(s) 596, 811, 897, 2122
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 3 cut(s) 11, 143, 410
BslFI GGGAC 3 cut(s) 268, 401, 596
BslI CCNNNNNNNGG 1 cut(s) 653
BsmAI GTCTC 2 cut(s) 254, 729
BsmFI GGGAC 3 cut(s) 268, 401, 596
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 6 cut(s) 10, 14, 338, 394, 903, 1500
Bso31I GGTCTC 2 cut(s) 254, 729
BsoBI CYCGRG 1 cut(s) 83
Bsp119I TTCGAA 1 cut(s) 458
Bsp1286I GDGCHC 4 cut(s) 596, 811, 897, 2122
Bsp1720I GCTNAGC 1 cut(s) 1217
Bsp19I CCATGG 1 cut(s) 1806
BspANI GGCC 6 cut(s) 10, 14, 338, 394, 903, 1500
BspCNI CTCAG 5 cut(s) 823, 1726, 1767, 1969, 2083
BspDI ATCGAT 1 cut(s) 192
BspFNI CGCG 3 cut(s) 37, 341, 1799
BspLI GGNNCC 7 cut(s) 240, 556, 695, 904, 973, 1146, 1553
BspMAI CTGCAG 2 cut(s) 314, 1078
BspMI ACCTGC 2 cut(s) 317, 615
BspOI GCTAGC 1 cut(s) 2087
BspPI GGATC 6 cut(s) 233, 246, 293, 1213, 1223, 1933
BspT104I TTCGAA 1 cut(s) 458
BspT107I GGYRCC 4 cut(s) 554, 693, 971, 1144
BspTNI GGTCTC 2 cut(s) 254, 729
BsrBI CCGCTC 1 cut(s) 209
BsrDI GCAATG 2 cut(s) 801, 1415
BsrFI RCCGGY 1 cut(s) 10
BsrI ACTGG 5 cut(s) 1053, 1233, 1441, 1609, 1924
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 6 cut(s) 550, 576, 577, 906, 1495, 1806
BssNI GRCGYC 1 cut(s) 1350
BssSI CACGAG 1 cut(s) 2115
BssT1I CCWWGG 2 cut(s) 1495, 1806
Bst2BI CACGAG 1 cut(s) 2115
Bst2UI CCWGG 6 cut(s) 202, 552, 578, 908, 1004, 2067
Bst4CI ACNGT 5 cut(s) 466, 741, 1082, 1507, 1577
BstACI GRCGYC 1 cut(s) 1350
BstAPI GCANNNNNTGC 3 cut(s) 988, 1073, 1082
BstBI TTCGAA 1 cut(s) 458
BstC8I GCNNGC 5 cut(s) 12, 1044, 1637, 1641, 2085
BstDEI CTNAG 7 cut(s) 810, 1217, 1628, 1713, 1754, 1977, 2091
BstDSI CCRYGG 1 cut(s) 1806
BstEII GGTNACC 1 cut(s) 998
BstF5I GGATG 2 cut(s) 778, 784
BstFNI CGCG 3 cut(s) 37, 341, 1799
BstH2I RGCGCY 2 cut(s) 318, 1646
BstHHI GCGC 4 cut(s) 39, 317, 1645, 1801
BstMAI GTCTC 2 cut(s) 254, 729
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 7 cut(s) 16, 206, 451, 988, 1073, 1082, 1645
BstNI CCWGG 6 cut(s) 202, 552, 578, 908, 1004, 2067
BstNSI RCATGY 2 cut(s) 1530, 1775
BstPAI GACNNNNGTC 1 cut(s) 1349
BstPI GGTNACC 1 cut(s) 998
BstSCI CCNGG 6 cut(s) 200, 550, 576, 906, 1002, 2065
BstSFI CTRYAG 3 cut(s) 310, 1074, 1932
BstSLI GKGCMC 1 cut(s) 2122
BstUI CGCG 3 cut(s) 37, 341, 1799
BstV1I GCAGC 6 cut(s) 324, 694, 697, 786, 1072, 1632
BstV2I GAAGAC 2 cut(s) 177, 1655
BstX2I RGATCY 4 cut(s) 238, 678, 1228, 1938
BstYI RGATCY 4 cut(s) 238, 678, 1228, 1938
BstZI CGGCCG 1 cut(s) 336
Bsu15I ATCGAT 1 cut(s) 192
Bsu36I CCTNAGG 1 cut(s) 1977
BsuI GTATCC 1 cut(s) 2007
BsuRI GGCC 6 cut(s) 10, 14, 338, 394, 903, 1500
BsuTUI ATCGAT 1 cut(s) 192
BtgI CCRYGG 1 cut(s) 1806
BtsCI GGATG 2 cut(s) 778, 784
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 8 cut(s) 462, 531, 1033, 1046, 1087, 1181, 1434, 2120
BveI ACCTGC 2 cut(s) 317, 615
Cac8I GCNNGC 5 cut(s) 12, 1044, 1637, 1641, 2085
CaiI CAGNNNCTG 1 cut(s) 1830
CfoI GCGC 4 cut(s) 39, 317, 1645, 1801
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 7 cut(s) 8, 13, 145, 204, 902, 1593, 2063
ClaI ATCGAT 1 cut(s) 192
CpoI CGGWCCG 1 cut(s) 145
CseI GACGC 1 cut(s) 1339
CsiI ACCWGGT 2 cut(s) 1002, 2065
Csp6I GTAC 6 cut(s) 4, 628, 1242, 1710, 1758, 1917
CspI CGGWCCG 1 cut(s) 145
CviQI GTAC 6 cut(s) 4, 628, 1242, 1710, 1758, 1917
DdeI CTNAG 7 cut(s) 810, 1217, 1628, 1713, 1754, 1977, 2091
DraIII CACNNNGTG 1 cut(s) 693
DrdI GACNNNNNNGTC 1 cut(s) 1007
DseDI GACNNNNNNGTC 1 cut(s) 1007
EaeI YGGCCR 2 cut(s) 336, 1498
EagI CGGCCG 1 cut(s) 336
Ecl136II GAGCTC 2 cut(s) 809, 895
EclXI CGGCCG 1 cut(s) 336
Eco130I CCWWGG 2 cut(s) 1495, 1806
Eco147I AGGCCT 1 cut(s) 394
Eco24I GRGCYC 2 cut(s) 811, 897
Eco31I GGTCTC 2 cut(s) 254, 729
Eco47I GGWCC 4 cut(s) 145, 204, 1593, 2063
Eco47III AGCGCT 1 cut(s) 1644
Eco52I CGGCCG 1 cut(s) 336
Eco53kI GAGCTC 2 cut(s) 809, 895
Eco57I CTGAAG 1 cut(s) 1122
Eco81I CCTNAGG 1 cut(s) 1977
Eco88I CYCGRG 1 cut(s) 83
Eco91I GGTNACC 1 cut(s) 998
EcoICRI GAGCTC 2 cut(s) 809, 895
EcoO65I GGTNACC 1 cut(s) 998
EcoRI GAATTC 1 cut(s) 1961
EcoRII CCWGG 6 cut(s) 200, 550, 576, 906, 1002, 2065
EcoT14I CCWWGG 2 cut(s) 1495, 1806
EcoT22I ATGCAT 1 cut(s) 853
EcoT38I GRGCYC 2 cut(s) 811, 897
ErhI CCWWGG 2 cut(s) 1495, 1806
FalI AAGNNNNNCTT 4 cut(s) 482, 514, 1095, 1127
FaqI GGGAC 3 cut(s) 268, 401, 596
FauI CCCGC 3 cut(s) 42, 276, 2008
FblI GTMKAC 1 cut(s) 1509
Fnu4HI GCNGC 9 cut(s) 313, 328, 339, 708, 711, 800, 1086, 1195, 1646
FokI GGATG 2 cut(s) 785, 791
FriOI GRGCYC 2 cut(s) 811, 897
FseI GGCCGGCC 1 cut(s) 14
Fsp4HI GCNGC 9 cut(s) 313, 328, 339, 708, 711, 800, 1086, 1195, 1646
FspBI CTAG 7 cut(s) 758, 803, 1374, 1481, 1580, 1704, 2084
GlaI GCGC 4 cut(s) 38, 316, 1644, 1800
GluI GCNGC 9 cut(s) 313, 328, 339, 708, 711, 800, 1086, 1195, 1646
GsaI CCCAGC 2 cut(s) 19, 276
GsuI CTGGAG 2 cut(s) 307, 735
HaeII RGCGCY 2 cut(s) 318, 1646
HaeIII GGCC 6 cut(s) 10, 14, 338, 394, 903, 1500
HapII CCGG 3 cut(s) 11, 143, 410
HgaI GACGC 1 cut(s) 1339
HhaI GCGC 4 cut(s) 39, 317, 1645, 1801
Hin1I GRCGYC 1 cut(s) 1350
Hin6I GCGC 4 cut(s) 37, 315, 1643, 1799
HinP1I GCGC 4 cut(s) 37, 315, 1643, 1799
HincII GTYRAC 2 cut(s) 535, 2041
HindII GTYRAC 2 cut(s) 535, 2041
HindIII AAGCTT 1 cut(s) 452
HpaI GTTAAC 1 cut(s) 2041
HpaII CCGG 3 cut(s) 11, 143, 410
HphI GGTGA 3 cut(s) 800, 1010, 2081
Hpy166II GTNNAC 7 cut(s) 112, 535, 1244, 1510, 1917, 2041, 2120
Hpy188I TCNGA 9 cut(s) 238, 297, 355, 823, 1239, 1524, 1597, 1687, 1716
Hpy188III TCNNGA 8 cut(s) 304, 664, 845, 1209, 1334, 1580, 1955, 2061
Hpy8I GTNNAC 7 cut(s) 112, 535, 1244, 1510, 1917, 2041, 2120
Hpy99I CGWCG 2 cut(s) 230, 233
HpyAV CCTTC 3 cut(s) 441, 666, 1540
HpyCH4III ACNGT 5 cut(s) 466, 741, 1082, 1507, 1577
HpyCH4IV ACGT 2 cut(s) 537, 864
HpyF10VI GCNNNNNNNGC 7 cut(s) 16, 206, 451, 988, 1073, 1082, 1645
HpyF3I CTNAG 7 cut(s) 810, 1217, 1628, 1713, 1754, 1977, 2091
HpySE526I ACGT 2 cut(s) 537, 864
Hsp92I GRCGYC 1 cut(s) 1350
HspAI GCGC 4 cut(s) 37, 315, 1643, 1799
KroI GCCGGC 1 cut(s) 10
KroNI GCCGGC 1 cut(s) 12
KspAI GTTAAC 1 cut(s) 2041
LmnI GCTCC 3 cut(s) 591, 806, 1610
Lsp1109I GCAGC 6 cut(s) 324, 694, 697, 786, 1072, 1632
LweI GCATC 4 cut(s) 781, 838, 1556, 2062
MabI ACCWGGT 2 cut(s) 1002, 2065
MaeI CTAG 7 cut(s) 758, 803, 1374, 1481, 1580, 1704, 2084
MaeII ACGT 2 cut(s) 537, 864
MaeIII GTNAC 9 cut(s) 331, 466, 998, 1334, 1369, 1394, 1476, 1767, 1792
MbiI CCGCTC 1 cut(s) 209
MfeI CAATTG 3 cut(s) 425, 1133, 2034
MflI RGATCY 4 cut(s) 238, 678, 1228, 1938
MhlI GDGCHC 4 cut(s) 596, 811, 897, 2122
MlsI TGGCCA 1 cut(s) 1500
MluNI TGGCCA 1 cut(s) 1500
MlyI GAGTC 2 cut(s) 131, 1873
MmeI TCCRAC 2 cut(s) 498, 750
Mox20I TGGCCA 1 cut(s) 1500
Mph1103I ATGCAT 1 cut(s) 853
MroNI GCCGGC 1 cut(s) 10
MroXI GAANNNNTTC 4 cut(s) 494, 1550, 1740, 1854
MscI TGGCCA 1 cut(s) 1500
MseI TTAA 3 cut(s) 1490, 2040, 2134
MslI CAYNNNNRTG 4 cut(s) 367, 566, 702, 2125
Msp20I TGGCCA 1 cut(s) 1500
MspA1I CMGCKG 2 cut(s) 276, 327
MspI CCGG 3 cut(s) 11, 143, 410
MspR9I CCNGG 6 cut(s) 202, 552, 578, 908, 1004, 2067
MunI CAATTG 3 cut(s) 425, 1133, 2034
Mva1269I GAATGC 1 cut(s) 419
MvaI CCWGG 6 cut(s) 202, 552, 578, 908, 1004, 2067
MvnI CGCG 3 cut(s) 37, 341, 1799
MwoI GCNNNNNNNGC 7 cut(s) 16, 206, 451, 988, 1073, 1082, 1645
NaeI GCCGGC 1 cut(s) 12
NcoI CCATGG 1 cut(s) 1806
NgoMIV GCCGGC 1 cut(s) 10
NheI GCTAGC 1 cut(s) 2083
NlaIV GGNNCC 7 cut(s) 240, 556, 695, 904, 973, 1146, 1553
NmuCI GTSAC 5 cut(s) 466, 998, 1334, 1476, 1767
NsiI ATGCAT 1 cut(s) 853
NspI RCATGY 2 cut(s) 1530, 1775
NspV TTCGAA 1 cut(s) 458
OliI CACNNNNGTG 1 cut(s) 2125
PasI CCCWGGG 1 cut(s) 577
PceI AGGCCT 1 cut(s) 394
PcsI WCGNNNNNNNCGW 1 cut(s) 733
PctI GAATGC 1 cut(s) 419
PdiI GCCGGC 1 cut(s) 12
PdmI GAANNNNTTC 4 cut(s) 494, 1550, 1740, 1854
PfeI GAWTC 8 cut(s) 233, 298, 476, 658, 824, 1274, 1827, 2097
PkrI GCNGC 9 cut(s) 314, 329, 340, 709, 712, 801, 1087, 1196, 1647
PleI GAGTC 2 cut(s) 131, 1873
PpsI GAGTC 2 cut(s) 131, 1873
PshAI GACNNNNGTC 1 cut(s) 1349
Psp124BI GAGCTC 2 cut(s) 811, 897
Psp1406I AACGTT 2 cut(s) 537, 864
Psp6I CCWGG 6 cut(s) 200, 550, 576, 906, 1002, 2065
PspEI GGTNACC 1 cut(s) 998
PspFI CCCAGC 2 cut(s) 15, 272
PspGI CCWGG 6 cut(s) 200, 550, 576, 906, 1002, 2065
PspN4I GGNNCC 7 cut(s) 240, 556, 695, 904, 973, 1146, 1553
PspPI GGNCC 7 cut(s) 8, 13, 145, 204, 902, 1593, 2063
PstI CTGCAG 2 cut(s) 314, 1078
PstNI CAGNNNCTG 1 cut(s) 1830
PsuI RGATCY 4 cut(s) 238, 678, 1228, 1938
PvuII CAGCTG 1 cut(s) 276
RigI GGCCGGCC 1 cut(s) 14
RsaI GTAC 6 cut(s) 5, 629, 1243, 1711, 1759, 1918
RsaNI GTAC 6 cut(s) 4, 628, 1242, 1710, 1758, 1917
RseI CAYNNNNRTG 4 cut(s) 367, 566, 702, 2125
Rsr2I CGGWCCG 1 cut(s) 145
RsrII CGGWCCG 1 cut(s) 145
SacI GAGCTC 2 cut(s) 811, 897
SaqAI TTAA 3 cut(s) 1490, 2040, 2134
SatI GCNGC 9 cut(s) 313, 328, 339, 708, 711, 800, 1086, 1195, 1646
Sau96I GGNCC 7 cut(s) 8, 13, 145, 204, 902, 1593, 2063
SchI GAGTC 2 cut(s) 131, 1873
ScrFI CCNGG 6 cut(s) 202, 552, 578, 908, 1004, 2067
SduI GDGCHC 4 cut(s) 596, 811, 897, 2122
SexAI ACCWGGT 2 cut(s) 1002, 2065
SfaNI GCATC 4 cut(s) 781, 838, 1556, 2062
SfcI CTRYAG 3 cut(s) 310, 1074, 1932
SfuI TTCGAA 1 cut(s) 458
SinI GGWCC 4 cut(s) 145, 204, 1593, 2063
SmiMI CAYNNNNRTG 4 cut(s) 367, 566, 702, 2125
SmlI CTYRAG 3 cut(s) 302, 662, 1254
SmoI CTYRAG 3 cut(s) 302, 662, 1254
SpeI ACTAGT 2 cut(s) 1373, 1703
SseBI AGGCCT 1 cut(s) 394
SspI AATATT 1 cut(s) 1472
SspMI CTAG 7 cut(s) 758, 803, 1374, 1481, 1580, 1704, 2084
SstI GAGCTC 2 cut(s) 811, 897
StuI AGGCCT 1 cut(s) 394
StyD4I CCNGG 6 cut(s) 200, 550, 576, 906, 1002, 2065
StyI CCWWGG 2 cut(s) 1495, 1806
TaaI ACNGT 5 cut(s) 466, 741, 1082, 1507, 1577
TaiI ACGT 2 cut(s) 540, 867
TaqI TCGA 6 cut(s) 135, 192, 225, 458, 736, 916
TatI WGTACW 4 cut(s) 1241, 1709, 1757, 1916
TauI GCSGC 3 cut(s) 330, 341, 1197
TfiI GAWTC 8 cut(s) 233, 298, 476, 658, 824, 1274, 1827, 2097
Tru1I TTAA 3 cut(s) 1490, 2040, 2134
Tru9I TTAA 3 cut(s) 1490, 2040, 2134
TscAI CASTG 8 cut(s) 469, 531, 1033, 1053, 1087, 1188, 1441, 2127
TseFI GTSAC 5 cut(s) 466, 998, 1334, 1476, 1767
TseI GCWGC 6 cut(s) 312, 707, 710, 799, 1085, 1645
Tsp45I GTSAC 5 cut(s) 466, 998, 1334, 1476, 1767
TspGWI ACGGA 2 cut(s) 261, 272
TspRI CASTG 8 cut(s) 469, 531, 1033, 1053, 1087, 1188, 1441, 2127
VneI GTGCAC 1 cut(s) 2118
VpaK11BI GGWCC 4 cut(s) 145, 204, 1593, 2063
XapI RAATTY 4 cut(s) 185, 493, 1115, 1961
XbaI TCTAGA 1 cut(s) 1579
XceI RCATGY 2 cut(s) 1530, 1775
XmiI GTMKAC 1 cut(s) 1509
XmnI GAANNNNTTC 4 cut(s) 494, 1550, 1740, 1854
XspI CTAG 7 cut(s) 758, 803, 1374, 1481, 1580, 1704, 2084
Zsp2I ATGCAT 1 cut(s) 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.