Rh4DG259000

Cell division control protein 24, OB domain 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014037
Physical Location & Seq
Forward (+)
47518212 .. 47522115
3904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG259000.1

Sequence Viewer

Length: 1605 bp
ATGAACTCACTCGACTCGCCGGACCGCAATCCGCAACCACAAGAAGAAGAAGACCCACTCCTCAAATTCATCGATTACGCCAGGTCCGCTCTCTCCCCTGAAATCGACGACGATTCGGATCCCAACGGAAATGGGAGGGAGACCGCCCGGCCCAGCTGGAATTGGATCGCCTCTCGGATTCTCAAGACCTGCAGCGCCTACTCCAGCGGCGTTACGGCCGCGATTTTGCTCTCCGATCTCTCACAGGATGATGATGGTGATGCAATGCTGCTAGGAGCTCAGTTTTTTTCAGATTCATTCTCCTCAATTTCTCTTGATGCATTTAGTAAAAACGTTTCTTATTCATTGTATGCAAGAATTGAGCTCATTGGCCCCCTGGAAGTTCGAGGAAAGTTTGGAACTTTACAAAGGAAACAAATCACTCTTGTAGATGATGATGGTGCCAAGTTGCAATTTCTGCTGTGGGGTGACCAGGTTGTCCTGGCAAATCTTTTCAGTGTGGGAAGTATGCTTGCACTGGATAAACCATATATTGCAAGTTCCGCAGACAGTGCTGCTGAAACAAGTGCTGAAGTTTCCCTTGAATTTGGTAGTGCAACACAATTGTTTTTGGTGCCTTTTGTTCAACACAAGGAACAAGTATGTGTATCACTGACACCAAGCCGCCATCAAGGATCAAGACTGCTAAGCACATTAGATCCCAGTCAGAGTACACAAGTTTCTCAAGTTTCCTTGCCCTGTGATTCACAAGGGTCTATTGATTTTAGTAGTTATCCTTTTCAATCCTACATTACCGATCTTCGTGACAAGATGACAGGCATCAGCCTTTATGGTGTTGTTACAAGTATCATCAAAGAAAGATGTACCCCAGCAGCTATCTTCTCATTGCGAATTGAAGATACCACTGGAACAATTTGGACTAAATTACATTTTGTGAAATATTGGTCACTAGGAAGATTAAGCCTTGGCCACACCGTCTACATATCCGGTCTGACATGCTCTATGAAAAAGCGGAAGGGGTTCCAGCTAAACAGATGCCATAACGGCCTAGAAGCAATATGGTCCGAGAATACTGGTGGAGCTTCTTTCTTCAACCTTAGTAGCTTGCCAGCGCTGCTAAACTCATCTTGTCTTCATAAACTAACAAAGCTCTCTGATCTGTCTAGCCAGTCTAGCTGTACTCAGATAGTTCATGTTCAGCTTGAAATGGTTCAATTTCATCTCAGTACAAGGTTGTCACATGCTGTATGTGGTCATTTTGTTACCGAGCAACCCATGGGAGTTTCTAAATGCAGCTTCTGTAATGAGAATTGTGATGAAGTAATTCGCACTTTCCACCTGAAAATGACTCTTGCTGACAAGAGTGGGAAAGTTTTTGCATGGTGTACTGGTCATACTGCTACAGAGATCCTGCAAATATCTCCTGATGAATTCTTTGAACTACCTGAGGAAGAACAAGTTATGTATCCCGCTTCATTAGATAATGAGAGGTTCATGGTTGCAATTGTTAACTGCAAAAGGCAAGGTTCTGGACCTGGTGATGCTCTGGTGCTAGCAACTGAGGGAATCTCATGGGAGATTACTCGTGCAGTGAAGTGTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDC24_OB1 CDC24_OB2 CDC24_OB3

Protein Analysis

534

Amino Acids

58.79

Weight (kDa)

5.14

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC24_OB1 PF17246 21 - 83 2e-20 Cell division control protein 24, OB domain 1
CDC24_OB2 PF17245 76 - 145 1.3e-16 Cell division control protein 24, OB domain 2
CDC24_OB3 PF17244 266 - 493 6.6e-77 Cell division control protein 24, OB domain 3
Rep_fac-A_C PF08646 415 - 506 2.4e-06 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17030
fragaria_vesca FvH4_4g19640
malus_domestica MD13G1155000.v1.1
prunus_persica Prupe.1G118000_v2.0.a1
pyrus_communis pycom13g13180
rosa_chinensis RchiOBHm_Chr4g0424901 RchiOBHm_Chr4g0424931
rosa_laevigata RLG00000007411
rosa_multiflora Rmu_sc0006754.1_g000002 Rmu_ssc0000420.1_g000005
rosa_roxburghii Rroxscaffold_5G00367120
rosa_rugosa Rorug04G0200500
rosa_samantha Rh4AG257900 Rh4BG263300 Rh4CG274300 Rh4DG259000
rosa_wichuraiana Rw4G022260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 476
Acc36I ACCTGC 1 cut(s) 197
AccB1I GGYRCC 2 cut(s) 440, 613
AccBSI CCGCTC 1 cut(s) 89
AccI GTMKAC 1 cut(s) 978
AccII CGCG 1 cut(s) 221
AclI AACGTT 1 cut(s) 333
AclWI GGATC 6 cut(s) 113, 126, 173, 682, 692, 1402
AcoI YGGCCR 2 cut(s) 216, 967
AcsI RAATTY 3 cut(s) 65, 584, 1430
AcuI CTGAAG 1 cut(s) 591
AfaI GTAC 5 cut(s) 712, 865, 1180, 1228, 1387
AfeI AGCGCT 1 cut(s) 1113
AgsI TTSAA 8 cut(s) 584, 626, 782, 896, 1093, 1205, 1214, 1439
AjnI CCWGG 5 cut(s) 80, 375, 471, 480, 1534
Alw21I GWGCWC 2 cut(s) 280, 366
Alw26I GTCTC 1 cut(s) 134
AlwI GGATC 6 cut(s) 113, 126, 173, 682, 692, 1402
AlwNI CAGNNNCTG 1 cut(s) 1299
Aor51HI AGCGCT 1 cut(s) 1113
AoxI GGCC 5 cut(s) 149, 216, 370, 967, 1045
ApeKI GCWGC 6 cut(s) 192, 268, 554, 872, 1114, 1293
ApoI RAATTY 3 cut(s) 65, 584, 1430
Asp700I GAANNNNTTC 3 cut(s) 1019, 1209, 1323
AspLEI GCGC 2 cut(s) 197, 1114
AspS9I GGNCC 6 cut(s) 22, 84, 150, 371, 1062, 1532
AsuC2I CCSGG 1 cut(s) 148
AsuHPI GGTGA 3 cut(s) 269, 479, 1550
AsuNHI GCTAGC 1 cut(s) 1552
AvaII GGWCC 4 cut(s) 22, 84, 1062, 1532
AxyI CCTNAGG 1 cut(s) 1446
BalI TGGCCA 1 cut(s) 969
BamHI GGATCC 1 cut(s) 118
BanI GGYRCC 2 cut(s) 440, 613
BanII GRGCYC 2 cut(s) 280, 366
BauI CACGAG 1 cut(s) 1584
BbsI GAAGAC 2 cut(s) 57, 1124
Bbv12I GWGCWC 2 cut(s) 280, 366
BbvI GCAGC 6 cut(s) 204, 255, 541, 884, 1101, 1305
BccI CCATC 3 cut(s) 248, 431, 675
BceAI ACGGC 2 cut(s) 231, 1060
BciT130I CCWGG 5 cut(s) 82, 377, 473, 482, 1536
BciVI GTATCC 1 cut(s) 1476
BcnI CCSGG 1 cut(s) 148
BcoDI GTCTC 1 cut(s) 134
BfaI CTAG 6 cut(s) 272, 950, 1049, 1164, 1173, 1553
BfmI CTRYAG 2 cut(s) 190, 1401
BfoI RGCGCY 2 cut(s) 198, 1115
BfuAI ACCTGC 1 cut(s) 197
BfuI GTATCC 1 cut(s) 1476
BglI GCCNNNNNGGC 1 cut(s) 1044
BisI GCNGC 9 cut(s) 193, 208, 219, 269, 555, 664, 873, 1115, 1294
BlpI GCTNAGC 1 cut(s) 686
BlsI GCNGC 9 cut(s) 194, 209, 220, 270, 556, 665, 874, 1116, 1295
Bme1390I CCNGG 6 cut(s) 82, 148, 377, 473, 482, 1536
Bme18I GGWCC 4 cut(s) 22, 84, 1062, 1532
BmgT120I GGNCC 6 cut(s) 22, 84, 150, 371, 1062, 1532
BmiI GGNNCC 5 cut(s) 120, 373, 442, 615, 1022
BmrFI CCNGG 6 cut(s) 82, 148, 377, 473, 482, 1536
BmrI ACTGGG 1 cut(s) 696
BmsI GCATC 5 cut(s) 250, 307, 828, 1025, 1531
BmtI GCTAGC 1 cut(s) 1556
BmuI ACTGGG 1 cut(s) 696
BpiI GAAGAC 2 cut(s) 57, 1124
BplI GAGNNNNNCTC 2 cut(s) 1553, 1585
BpmI CTGGAG 1 cut(s) 187
Bpu1102I GCTNAGC 1 cut(s) 686
BpuEI CTTGAG 2 cut(s) 167, 708
BpuMI CCSGG 1 cut(s) 148
Bsa29I ATCGAT 1 cut(s) 72
BsaBI GATNNNNATC 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 3 cut(s) 375, 964, 1275
BsaWI WCCGGW 1 cut(s) 986
Bse1I ACTGG 6 cut(s) 522, 702, 910, 1078, 1168, 1393
Bse21I CCTNAGG 1 cut(s) 1446
Bse3DI GCAATG 2 cut(s) 270, 884
Bse8I GATNNNNATC 1 cut(s) 117
BseBI CCWGG 5 cut(s) 82, 377, 473, 482, 1536
BseCI ATCGAT 1 cut(s) 72
BseDI CCNNGG 3 cut(s) 375, 964, 1275
BseGI GGATG 1 cut(s) 253
BseJI GATNNNNATC 1 cut(s) 117
BseMI GCAATG 2 cut(s) 270, 884
BseMII CTCAG 5 cut(s) 293, 1196, 1237, 1437, 1551
BseNI ACTGG 6 cut(s) 522, 702, 910, 1078, 1168, 1393
BseRI GAGGAG 2 cut(s) 50, 292
BseX3I CGGCCG 1 cut(s) 216
BseXI GCAGC 6 cut(s) 204, 255, 541, 884, 1101, 1305
BseYI CCCAGC 2 cut(s) 152, 868
Bsh1236I CGCG 1 cut(s) 221
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 5 cut(s) 151, 218, 372, 969, 1047
BshNI GGYRCC 2 cut(s) 440, 613
BshVI ATCGAT 1 cut(s) 72
BsiEI CGRYCG 1 cut(s) 219
BsiHKAI GWGCWC 2 cut(s) 280, 366
BsiSI CCGG 3 cut(s) 20, 148, 987
BsmAI GTCTC 1 cut(s) 134
BsnI GGCC 5 cut(s) 151, 218, 372, 969, 1047
Bso31I GGTCTC 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 280, 366
Bsp143I GATC 8 cut(s) 118, 165, 235, 674, 697, 796, 1156, 1407
Bsp1720I GCTNAGC 1 cut(s) 686
Bsp19I CCATGG 1 cut(s) 1275
BspANI GGCC 5 cut(s) 151, 218, 372, 969, 1047
BspCNI CTCAG 5 cut(s) 292, 1195, 1236, 1438, 1552
BspDI ATCGAT 1 cut(s) 72
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 5 cut(s) 120, 373, 442, 615, 1022
BspMAI CTGCAG 1 cut(s) 194
BspMI ACCTGC 1 cut(s) 197
BspOI GCTAGC 1 cut(s) 1556
BspPI GGATC 6 cut(s) 113, 126, 173, 682, 692, 1402
BspT107I GGYRCC 2 cut(s) 440, 613
BspTNI GGTCTC 1 cut(s) 134
BsrBI CCGCTC 1 cut(s) 89
BsrDI GCAATG 2 cut(s) 270, 884
BsrI ACTGG 6 cut(s) 522, 702, 910, 1078, 1168, 1393
BssECI CCNNGG 3 cut(s) 375, 964, 1275
BssMI GATC 8 cut(s) 118, 165, 235, 674, 697, 796, 1156, 1407
BssSI CACGAG 1 cut(s) 1584
BssT1I CCWWGG 2 cut(s) 964, 1275
Bst2BI CACGAG 1 cut(s) 1584
Bst2UI CCWGG 5 cut(s) 82, 377, 473, 482, 1536
Bst4CI ACNGT 2 cut(s) 551, 976
BstAPI GCANNNNNTGC 2 cut(s) 457, 551
BstC8I GCNNGC 4 cut(s) 513, 1106, 1110, 1554
BstDEI CTNAG 7 cut(s) 279, 686, 1097, 1182, 1223, 1446, 1560
BstDSI CCRYGG 1 cut(s) 1275
BstEII GGTNACC 1 cut(s) 467
BstF5I GGATG 1 cut(s) 253
BstFNI CGCG 1 cut(s) 221
BstH2I RGCGCY 2 cut(s) 198, 1115
BstHHI GCGC 2 cut(s) 197, 1114
BstKTI GATC 8 cut(s) 121, 168, 238, 677, 700, 799, 1159, 1410
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 8 cut(s) 118, 165, 235, 674, 697, 796, 1156, 1407
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 7 cut(s) 86, 457, 542, 551, 1044, 1114, 1173
BstNI CCWGG 5 cut(s) 82, 377, 473, 482, 1536
BstNSI RCATGY 2 cut(s) 999, 1244
BstPI GGTNACC 1 cut(s) 467
BstSCI CCNGG 6 cut(s) 80, 146, 375, 471, 480, 1534
BstSFI CTRYAG 2 cut(s) 190, 1401
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 6 cut(s) 204, 255, 541, 884, 1101, 1305
BstV2I GAAGAC 2 cut(s) 57, 1124
BstX2I RGATCY 3 cut(s) 118, 697, 1407
BstYI RGATCY 3 cut(s) 118, 697, 1407
BstZI CGGCCG 1 cut(s) 216
Bsu15I ATCGAT 1 cut(s) 72
Bsu36I CCTNAGG 1 cut(s) 1446
BsuI GTATCC 1 cut(s) 1476
BsuRI GGCC 5 cut(s) 151, 218, 372, 969, 1047
BsuTUI ATCGAT 1 cut(s) 72
BtgI CCRYGG 1 cut(s) 1275
BtsCI GGATG 1 cut(s) 253
BtsI GCAGTG 1 cut(s) 1596
BtsIMutI CAGTG 6 cut(s) 502, 515, 556, 650, 903, 1596
BveI ACCTGC 1 cut(s) 197
Cac8I GCNNGC 4 cut(s) 513, 1106, 1110, 1554
CaiI CAGNNNCTG 1 cut(s) 1299
CfoI GCGC 2 cut(s) 197, 1114
Cfr13I GGNCC 6 cut(s) 22, 84, 150, 371, 1062, 1532
ClaI ATCGAT 1 cut(s) 72
CpoI CGGWCCG 1 cut(s) 22
CsiI ACCWGGT 2 cut(s) 471, 1534
Csp6I GTAC 5 cut(s) 711, 864, 1179, 1227, 1386
CspI CGGWCCG 1 cut(s) 22
CviAII CATG 7 cut(s) 996, 1193, 1241, 1276, 1380, 1495, 1572
CviQI GTAC 5 cut(s) 711, 864, 1179, 1227, 1386
DdeI CTNAG 7 cut(s) 279, 686, 1097, 1182, 1223, 1446, 1560
DpnI GATC 8 cut(s) 120, 167, 237, 676, 699, 798, 1158, 1409
DpnII GATC 8 cut(s) 118, 165, 235, 674, 697, 796, 1156, 1407
DrdI GACNNNNNNGTC 1 cut(s) 476
DseDI GACNNNNNNGTC 1 cut(s) 476
EaeI YGGCCR 2 cut(s) 216, 967
EagI CGGCCG 1 cut(s) 216
Ecl136II GAGCTC 2 cut(s) 278, 364
EclXI CGGCCG 1 cut(s) 216
Eco130I CCWWGG 2 cut(s) 964, 1275
Eco24I GRGCYC 2 cut(s) 280, 366
Eco31I GGTCTC 1 cut(s) 134
Eco47I GGWCC 4 cut(s) 22, 84, 1062, 1532
Eco47III AGCGCT 1 cut(s) 1113
Eco52I CGGCCG 1 cut(s) 216
Eco53kI GAGCTC 2 cut(s) 278, 364
Eco57I CTGAAG 1 cut(s) 591
Eco81I CCTNAGG 1 cut(s) 1446
Eco91I GGTNACC 1 cut(s) 467
EcoICRI GAGCTC 2 cut(s) 278, 364
EcoO65I GGTNACC 1 cut(s) 467
EcoRI GAATTC 1 cut(s) 1430
EcoRII CCWGG 5 cut(s) 80, 375, 471, 480, 1534
EcoT14I CCWWGG 2 cut(s) 964, 1275
EcoT22I ATGCAT 1 cut(s) 322
EcoT38I GRGCYC 2 cut(s) 280, 366
ErhI CCWWGG 2 cut(s) 964, 1275
FaeI CATG 7 cut(s) 999, 1196, 1244, 1279, 1383, 1498, 1575
FalI AAGNNNNNCTT 2 cut(s) 564, 596
FatI CATG 7 cut(s) 995, 1192, 1240, 1275, 1379, 1494, 1571
FauI CCCGC 1 cut(s) 1477
FblI GTMKAC 1 cut(s) 978
Fnu4HI GCNGC 9 cut(s) 193, 208, 219, 269, 555, 664, 873, 1115, 1294
FokI GGATG 1 cut(s) 260
FriOI GRGCYC 2 cut(s) 280, 366
Fsp4HI GCNGC 9 cut(s) 193, 208, 219, 269, 555, 664, 873, 1115, 1294
FspBI CTAG 6 cut(s) 272, 950, 1049, 1164, 1173, 1553
GlaI GCGC 2 cut(s) 196, 1113
GluI GCNGC 9 cut(s) 193, 208, 219, 269, 555, 664, 873, 1115, 1294
GsaI CCCAGC 2 cut(s) 156, 872
GsuI CTGGAG 1 cut(s) 187
HaeII RGCGCY 2 cut(s) 198, 1115
HaeIII GGCC 5 cut(s) 151, 218, 372, 969, 1047
HapII CCGG 3 cut(s) 20, 148, 987
HhaI GCGC 2 cut(s) 197, 1114
Hin1II CATG 7 cut(s) 999, 1196, 1244, 1279, 1383, 1498, 1575
Hin6I GCGC 2 cut(s) 195, 1112
HinP1I GCGC 2 cut(s) 195, 1112
HincII GTYRAC 1 cut(s) 1510
HindII GTYRAC 1 cut(s) 1510
HinfI GANTC 7 cut(s) 14, 113, 178, 293, 743, 1348, 1566
HpaI GTTAAC 1 cut(s) 1510
HpaII CCGG 3 cut(s) 20, 148, 987
HphI GGTGA 3 cut(s) 269, 479, 1550
Hpy166II GTNNAC 4 cut(s) 713, 979, 1386, 1510
Hpy188I TCNGA 9 cut(s) 118, 177, 235, 292, 708, 993, 1066, 1156, 1185
Hpy188III TCNNGA 6 cut(s) 184, 314, 678, 803, 1424, 1530
Hpy8I GTNNAC 4 cut(s) 713, 979, 1386, 1510
Hpy99I CGWCG 2 cut(s) 110, 113
HpyAV CCTTC 1 cut(s) 1009
HpyCH4III ACNGT 2 cut(s) 551, 976
HpyCH4IV ACGT 1 cut(s) 333
HpyF10VI GCNNNNNNNGC 7 cut(s) 86, 457, 542, 551, 1044, 1114, 1173
HpyF3I CTNAG 7 cut(s) 279, 686, 1097, 1182, 1223, 1446, 1560
HpySE526I ACGT 1 cut(s) 333
Hsp92II CATG 7 cut(s) 999, 1196, 1244, 1279, 1383, 1498, 1575
HspAI GCGC 2 cut(s) 195, 1112
KspAI GTTAAC 1 cut(s) 1510
Kzo9I GATC 8 cut(s) 118, 165, 235, 674, 697, 796, 1156, 1407
LmnI GCTCC 2 cut(s) 275, 1079
Lsp1109I GCAGC 6 cut(s) 204, 255, 541, 884, 1101, 1305
LweI GCATC 5 cut(s) 250, 307, 828, 1025, 1531
MabI ACCWGGT 2 cut(s) 471, 1534
MaeI CTAG 6 cut(s) 272, 950, 1049, 1164, 1173, 1553
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 7 cut(s) 211, 467, 803, 838, 945, 1236, 1261
MalI GATC 8 cut(s) 120, 167, 237, 676, 699, 798, 1158, 1409
MbiI CCGCTC 1 cut(s) 89
MboI GATC 8 cut(s) 118, 165, 235, 674, 697, 796, 1156, 1407
MfeI CAATTG 2 cut(s) 602, 1503
MflI RGATCY 3 cut(s) 118, 697, 1407
MhlI GDGCHC 2 cut(s) 280, 366
MlsI TGGCCA 1 cut(s) 969
MluNI TGGCCA 1 cut(s) 969
MlyI GAGTC 2 cut(s) 8, 1342
MnlI CCTC 8 cut(s) 71, 129, 181, 313, 380, 1441, 1482, 1555
Mox20I TGGCCA 1 cut(s) 969
Mph1103I ATGCAT 1 cut(s) 322
MroXI GAANNNNTTC 3 cut(s) 1019, 1209, 1323
MscI TGGCCA 1 cut(s) 969
MseI TTAA 3 cut(s) 959, 1509, 1603
Msp20I TGGCCA 1 cut(s) 969
MspA1I CMGCKG 2 cut(s) 156, 207
MspI CCGG 3 cut(s) 20, 148, 987
MspR9I CCNGG 6 cut(s) 82, 148, 377, 473, 482, 1536
MunI CAATTG 2 cut(s) 602, 1503
MvaI CCWGG 5 cut(s) 82, 377, 473, 482, 1536
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 7 cut(s) 86, 457, 542, 551, 1044, 1114, 1173
NciI CCSGG 1 cut(s) 148
NcoI CCATGG 1 cut(s) 1275
NdeII GATC 8 cut(s) 118, 165, 235, 674, 697, 796, 1156, 1407
NheI GCTAGC 1 cut(s) 1552
NlaIII CATG 7 cut(s) 999, 1196, 1244, 1279, 1383, 1498, 1575
NlaIV GGNNCC 5 cut(s) 120, 373, 442, 615, 1022
NmuCI GTSAC 4 cut(s) 467, 803, 945, 1236
NsiI ATGCAT 1 cut(s) 322
NspI RCATGY 2 cut(s) 999, 1244
PdmI GAANNNNTTC 3 cut(s) 1019, 1209, 1323
PfeI GAWTC 5 cut(s) 113, 178, 293, 743, 1566
PkrI GCNGC 9 cut(s) 194, 209, 220, 270, 556, 665, 874, 1116, 1295
PleI GAGTC 2 cut(s) 8, 1342
PpsI GAGTC 2 cut(s) 8, 1342
Psp124BI GAGCTC 2 cut(s) 280, 366
Psp1406I AACGTT 1 cut(s) 333
Psp6I CCWGG 5 cut(s) 80, 375, 471, 480, 1534
PspEI GGTNACC 1 cut(s) 467
PspFI CCCAGC 2 cut(s) 152, 868
PspGI CCWGG 5 cut(s) 80, 375, 471, 480, 1534
PspN4I GGNNCC 5 cut(s) 120, 373, 442, 615, 1022
PspPI GGNCC 6 cut(s) 22, 84, 150, 371, 1062, 1532
PstI CTGCAG 1 cut(s) 194
PstNI CAGNNNCTG 1 cut(s) 1299
PsuI RGATCY 3 cut(s) 118, 697, 1407
PvuII CAGCTG 1 cut(s) 156
RsaI GTAC 5 cut(s) 712, 865, 1180, 1228, 1387
RsaNI GTAC 5 cut(s) 711, 864, 1179, 1227, 1386
Rsr2I CGGWCCG 1 cut(s) 22
RsrII CGGWCCG 1 cut(s) 22
SacI GAGCTC 2 cut(s) 280, 366
SaqAI TTAA 3 cut(s) 959, 1509, 1603
SatI GCNGC 9 cut(s) 193, 208, 219, 269, 555, 664, 873, 1115, 1294
Sau3AI GATC 8 cut(s) 118, 165, 235, 674, 697, 796, 1156, 1407
Sau96I GGNCC 6 cut(s) 22, 84, 150, 371, 1062, 1532
SchI GAGTC 2 cut(s) 8, 1342
ScrFI CCNGG 6 cut(s) 82, 148, 377, 473, 482, 1536
SduI GDGCHC 2 cut(s) 280, 366
SexAI ACCWGGT 2 cut(s) 471, 1534
SfaNI GCATC 5 cut(s) 250, 307, 828, 1025, 1531
SfcI CTRYAG 2 cut(s) 190, 1401
SinI GGWCC 4 cut(s) 22, 84, 1062, 1532
SmlI CTYRAG 2 cut(s) 182, 723
SmoI CTYRAG 2 cut(s) 182, 723
SspI AATATT 1 cut(s) 941
SspMI CTAG 6 cut(s) 272, 950, 1049, 1164, 1173, 1553
SstI GAGCTC 2 cut(s) 280, 366
StyD4I CCNGG 6 cut(s) 80, 146, 375, 471, 480, 1534
StyI CCWWGG 2 cut(s) 964, 1275
TaaI ACNGT 2 cut(s) 551, 976
TaiI ACGT 1 cut(s) 336
TaqI TCGA 4 cut(s) 12, 72, 105, 385
TatI WGTACW 4 cut(s) 710, 1178, 1226, 1385
TauI GCSGC 3 cut(s) 210, 221, 666
TfiI GAWTC 5 cut(s) 113, 178, 293, 743, 1566
Tru1I TTAA 3 cut(s) 959, 1509, 1603
Tru9I TTAA 3 cut(s) 959, 1509, 1603
TscAI CASTG 6 cut(s) 502, 522, 556, 657, 910, 1596
TseFI GTSAC 4 cut(s) 467, 803, 945, 1236
TseI GCWGC 6 cut(s) 192, 268, 554, 872, 1114, 1293
Tsp45I GTSAC 4 cut(s) 467, 803, 945, 1236
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 6 cut(s) 502, 522, 556, 657, 910, 1596
VpaK11BI GGWCC 4 cut(s) 22, 84, 1062, 1532
XapI RAATTY 3 cut(s) 65, 584, 1430
XceI RCATGY 2 cut(s) 999, 1244
XmiI GTMKAC 1 cut(s) 978
XmnI GAANNNNTTC 3 cut(s) 1019, 1209, 1323
XspI CTAG 6 cut(s) 272, 950, 1049, 1164, 1173, 1553
Zsp2I ATGCAT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.