Rh4CG274300

Cell division control protein 24, OB domain 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0014037
Physical Location & Seq
Forward (+)
52880389 .. 52884265
3877 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG274300.1

Sequence Viewer

Length: 1704 bp
ATGAACTCACTCGACTCGCCGGACCGCAATCCGCAACCACAAGAAGAAGACCCACTCCTCAAATTCATCGATTACGCCAGGTCCGCTCTCTCCCCTGAAATCGACGACGATTCGGATCCCAACGGAAATGGGACGGAGACCGCCCCGCCCAGCTGGAATTGGATCGCCTCTCGGATTCTCAAGACCTGCAGCGCCTACTCCAGCGGCGTTACGGCCGCGATTTTGCTCTCCGATCTCTCACAGAAAGGAAGGCAGATCTTCCTCACAGGGTGCCATCTTCGTGCTGCTGCTGGAGGAGGGTCTCGTTGTCCTCGACTGTTGCCAACAGAATATCTAGTGATATTGTTGGATGAGGATGATGATGGTGATGCAATGCTGCTAGGAGCTCAGTTTTGTTCAGATTCATTCTCCTCAATTTCTCTTGACGCATTTAGTAAAAACGTTTCTTATTCATTGTATGCAAGAATTGAGCTCATTGGCCCCCTGGAAGTTCGAGGAAAGTTTGGAACTTTACAAAGGAAACAAATCACTCTTGTAGATGATGATGGTGCCAAGTTGCAATTTCTGCTGTGGGGTGACCAGGTTGTCCTGGCAAATCTTTTCAGTGTGGGAAGTATGCTTGCACTGGATAAACCATATATTGCAAGTTCCGCAGACAGTGCTGCTGAAACAAGTGCTGAAGTTTCCCTTGAATTTGGTAGTGCAACACAATTGTTTTTGGTGCCTTTTGTTCAACACAAGGAACAAGTATGTGTATCACTGACACCAAGCCGCCATCAAGGATCAAGACTGCTAAGCACATTAGATCCCAGTCAGAGTACACAAGTTTCCTTGCCCTGTGATTCACAAGGGTCTATTGATTTTAGTAGTTATCCTTTTCAATCCTACATTACCGATCTTCGTGACAAGATGACAGGCGTCAGCCTTTACGGTGTTGTTACAAGTATCATCAAAGAAAGATGTACCACAGAAGCTATCTTCTCATTGCGAATTGAAGATACCACTGGAACAATTTGGACTAAATTACATTTTGTGAAATATTGGTCACTAGGAAGATTAAGCCTTGGCCACACCGTCTACATATCCGGTCTGACATGCTCTATGAAAAAGCGGAAGGGGTTCCAGCTAAACAGATGCCATGACGGCCTAGAAGTAATATGGTCCGAGAATACTGGTGGAGCTTCTTTCTTCAACCTTAGTAGCTTGCCAGCGCTGCTAAACTCATCTTGTCTTCATAAACTAACAAAGCTCTCTGATCTGTCCAGCCAGACTAGCTGTACTCAGATAGTTCATGTTCAGCTTGAAATGGTTCAATTTAATCTCAGTACAAGGTTGTCACATGCTGTATGTGGTCATTTTGTTACCGAGCAACCCATGGGAGTTTCTAAATGCAGCTTCTGTAATGAGAATTGTGATGAAGTAATTCGCACTTTCCACCTGAAAATGACTCTTGCTGACAAGAGTGGGAAAGTTTTTGCATGGTGTACTGGTCATACTGCTACAGAGATCCTGCAAATATCTCCTGATGAATTCTTTGAACTACCTGAGGAAGAACAAGTTATGTATCCCGCTTCATTAGATAATGAGAGGTTCATGGTTGCAATTGTCAACTGCAAAACGCAAGGTTCTGGACCTGGTGATGCTCTGGTGCTAGCAACTGAGGGAATCTCATGGGAGATTACTCGTGCACTGAAGTGCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDC24_OB1 CDC24_OB2 CDC24_OB3

Protein Analysis

567

Amino Acids

62.22

Weight (kDa)

5.06

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC24_OB1 PF17246 20 - 84 9.6e-21 Cell division control protein 24, OB domain 1
CDC24_OB2 PF17245 81 - 181 5.6e-33 Cell division control protein 24, OB domain 2
CDC24_OB3 PF17244 299 - 526 1e-77 Cell division control protein 24, OB domain 3
Rep_fac-A_C PF08646 449 - 540 1.5e-06 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17030
fragaria_vesca FvH4_4g19640
malus_domestica MD13G1155000.v1.1
prunus_persica Prupe.1G118000_v2.0.a1
pyrus_communis pycom13g13180
rosa_chinensis RchiOBHm_Chr4g0424901 RchiOBHm_Chr4g0424931
rosa_laevigata RLG00000007411
rosa_multiflora Rmu_sc0006754.1_g000002 Rmu_ssc0000420.1_g000005
rosa_roxburghii Rroxscaffold_5G00367120
rosa_rugosa Rorug04G0200500
rosa_samantha Rh4AG257900 Rh4BG263300 Rh4CG274300 Rh4DG259000
rosa_wichuraiana Rw4G022260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 584
Acc36I ACCTGC 1 cut(s) 194
AccB1I GGYRCC 3 cut(s) 270, 548, 721
AccBSI CCGCTC 1 cut(s) 86
AccI GTMKAC 1 cut(s) 1077
AccII CGCG 1 cut(s) 218
AclI AACGTT 1 cut(s) 441
AclWI GGATC 6 cut(s) 110, 123, 170, 790, 800, 1501
AcoI YGGCCR 2 cut(s) 213, 1066
AcsI RAATTY 3 cut(s) 62, 692, 1529
AcuI CTGAAG 1 cut(s) 699
AcyI GRCGYC 1 cut(s) 918
AdeI CACNNNGTG 1 cut(s) 270
AfaI GTAC 5 cut(s) 820, 964, 1279, 1327, 1486
AfeI AGCGCT 1 cut(s) 1212
AgsI TTSAA 8 cut(s) 692, 734, 881, 995, 1192, 1304, 1313, 1538
AjnI CCWGG 5 cut(s) 77, 483, 579, 588, 1633
AleI CACNNNNGTG 1 cut(s) 1693
Alw21I GWGCWC 3 cut(s) 388, 474, 1690
Alw26I GTCTC 2 cut(s) 131, 306
Alw44I GTGCAC 1 cut(s) 1686
AlwI GGATC 6 cut(s) 110, 123, 170, 790, 800, 1501
AlwNI CAGNNNCTG 1 cut(s) 1398
Aor51HI AGCGCT 1 cut(s) 1212
AoxI GGCC 4 cut(s) 213, 478, 1066, 1144
ApaLI GTGCAC 1 cut(s) 1686
ApeKI GCWGC 7 cut(s) 189, 284, 287, 376, 662, 1213, 1392
ApoI RAATTY 3 cut(s) 62, 692, 1529
Asp700I GAANNNNTTC 3 cut(s) 1118, 1308, 1422
AspLEI GCGC 2 cut(s) 194, 1213
AspS9I GGNCC 5 cut(s) 22, 81, 479, 1161, 1631
AsuHPI GGTGA 3 cut(s) 377, 587, 1649
AsuNHI GCTAGC 1 cut(s) 1651
AvaII GGWCC 4 cut(s) 22, 81, 1161, 1631
AxyI CCTNAGG 1 cut(s) 1545
BaeGI GKGCMC 1 cut(s) 1690
BalI TGGCCA 1 cut(s) 1068
BamHI GGATCC 1 cut(s) 115
BanI GGYRCC 3 cut(s) 270, 548, 721
BanII GRGCYC 2 cut(s) 388, 474
BauI CACGAG 1 cut(s) 1683
BbsI GAAGAC 2 cut(s) 54, 1223
Bbv12I GWGCWC 3 cut(s) 388, 474, 1690
BbvI GCAGC 7 cut(s) 201, 271, 274, 363, 649, 1200, 1404
BccI CCATC 4 cut(s) 282, 356, 539, 783
BceAI ACGGC 2 cut(s) 228, 1159
BciT130I CCWGG 5 cut(s) 79, 485, 581, 590, 1635
BciVI GTATCC 1 cut(s) 1575
BcoDI GTCTC 2 cut(s) 131, 306
BfaI CTAG 6 cut(s) 335, 380, 1049, 1148, 1272, 1652
BfmI CTRYAG 2 cut(s) 187, 1500
BfoI RGCGCY 2 cut(s) 195, 1214
BfuAI ACCTGC 1 cut(s) 194
BfuI GTATCC 1 cut(s) 1575
BglI GCCNNNNNGGC 1 cut(s) 1143
BglII AGATCT 1 cut(s) 255
BlpI GCTNAGC 1 cut(s) 794
Bme1390I CCNGG 5 cut(s) 79, 485, 581, 590, 1635
Bme18I GGWCC 4 cut(s) 22, 81, 1161, 1631
BmgT120I GGNCC 5 cut(s) 22, 81, 479, 1161, 1631
BmiI GGNNCC 6 cut(s) 117, 272, 481, 550, 723, 1121
BmrFI CCNGG 5 cut(s) 79, 485, 581, 590, 1635
BmrI ACTGGG 1 cut(s) 804
BmsI GCATC 3 cut(s) 358, 1124, 1630
BmtI GCTAGC 1 cut(s) 1655
BmuI ACTGGG 1 cut(s) 804
BoxI GACNNNNGTC 1 cut(s) 917
BpiI GAAGAC 2 cut(s) 54, 1223
BplI GAGNNNNNCTC 2 cut(s) 1652, 1684
BpmI CTGGAG 2 cut(s) 184, 312
Bpu1102I GCTNAGC 1 cut(s) 794
BpuEI CTTGAG 1 cut(s) 164
Bsa29I ATCGAT 1 cut(s) 69
BsaBI GATNNNNATC 1 cut(s) 114
BsaHI GRCGYC 1 cut(s) 918
BsaI GGTCTC 2 cut(s) 131, 306
BsaJI CCNNGG 3 cut(s) 483, 1063, 1374
BsaWI WCCGGW 1 cut(s) 1085
Bse1I ACTGG 5 cut(s) 630, 810, 1009, 1177, 1492
Bse21I CCTNAGG 1 cut(s) 1545
Bse3DI GCAATG 2 cut(s) 378, 983
Bse8I GATNNNNATC 1 cut(s) 114
BseBI CCWGG 5 cut(s) 79, 485, 581, 590, 1635
BseCI ATCGAT 1 cut(s) 69
BseDI CCNNGG 3 cut(s) 483, 1063, 1374
BseGI GGATG 2 cut(s) 355, 361
BseJI GATNNNNATC 1 cut(s) 114
BseMI GCAATG 2 cut(s) 378, 983
BseMII CTCAG 5 cut(s) 401, 1295, 1336, 1536, 1650
BseNI ACTGG 5 cut(s) 630, 810, 1009, 1177, 1492
BseRI GAGGAG 3 cut(s) 47, 309, 400
BseSI GKGCMC 1 cut(s) 1690
BseX3I CGGCCG 1 cut(s) 213
BseXI GCAGC 7 cut(s) 201, 271, 274, 363, 649, 1200, 1404
BseYI CCCAGC 1 cut(s) 149
Bsh1236I CGCG 1 cut(s) 218
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 4 cut(s) 215, 480, 1068, 1146
BshNI GGYRCC 3 cut(s) 270, 548, 721
BshVI ATCGAT 1 cut(s) 69
BsiEI CGRYCG 1 cut(s) 216
BsiHKAI GWGCWC 3 cut(s) 388, 474, 1690
BsiSI CCGG 2 cut(s) 20, 1086
BslFI GGGAC 1 cut(s) 145
BsmAI GTCTC 2 cut(s) 131, 306
BsmFI GGGAC 1 cut(s) 145
BsnI GGCC 4 cut(s) 215, 480, 1068, 1146
Bso31I GGTCTC 2 cut(s) 131, 306
Bsp1286I GDGCHC 3 cut(s) 388, 474, 1690
Bsp143I GATC 9 cut(s) 115, 162, 232, 255, 782, 805, 895, 1255, 1506
Bsp1720I GCTNAGC 1 cut(s) 794
Bsp19I CCATGG 1 cut(s) 1374
BspANI GGCC 4 cut(s) 215, 480, 1068, 1146
BspCNI CTCAG 5 cut(s) 400, 1294, 1335, 1537, 1651
BspDI ATCGAT 1 cut(s) 69
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 6 cut(s) 117, 272, 481, 550, 723, 1121
BspMAI CTGCAG 1 cut(s) 191
BspMI ACCTGC 1 cut(s) 194
BspOI GCTAGC 1 cut(s) 1655
BspPI GGATC 6 cut(s) 110, 123, 170, 790, 800, 1501
BspT107I GGYRCC 3 cut(s) 270, 548, 721
BspTNI GGTCTC 2 cut(s) 131, 306
BsrBI CCGCTC 1 cut(s) 86
BsrDI GCAATG 2 cut(s) 378, 983
BsrI ACTGG 5 cut(s) 630, 810, 1009, 1177, 1492
BssECI CCNNGG 3 cut(s) 483, 1063, 1374
BssMI GATC 9 cut(s) 115, 162, 232, 255, 782, 805, 895, 1255, 1506
BssNI GRCGYC 1 cut(s) 918
BssSI CACGAG 1 cut(s) 1683
BssT1I CCWWGG 2 cut(s) 1063, 1374
Bst2BI CACGAG 1 cut(s) 1683
Bst2UI CCWGG 5 cut(s) 79, 485, 581, 590, 1635
Bst4CI ACNGT 4 cut(s) 318, 659, 932, 1075
BstACI GRCGYC 1 cut(s) 918
BstAPI GCANNNNNTGC 2 cut(s) 565, 659
BstC8I GCNNGC 4 cut(s) 621, 1205, 1209, 1653
BstDEI CTNAG 7 cut(s) 387, 794, 1196, 1281, 1322, 1545, 1659
BstDSI CCRYGG 1 cut(s) 1374
BstEII GGTNACC 1 cut(s) 575
BstF5I GGATG 2 cut(s) 355, 361
BstFNI CGCG 1 cut(s) 218
BstH2I RGCGCY 2 cut(s) 195, 1214
BstHHI GCGC 2 cut(s) 194, 1213
BstKTI GATC 9 cut(s) 118, 165, 235, 258, 785, 808, 898, 1258, 1509
BstMAI GTCTC 2 cut(s) 131, 306
BstMBI GATC 9 cut(s) 115, 162, 232, 255, 782, 805, 895, 1255, 1506
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 7 cut(s) 83, 565, 650, 659, 1143, 1213, 1272
BstNI CCWGG 5 cut(s) 79, 485, 581, 590, 1635
BstNSI RCATGY 2 cut(s) 1098, 1343
BstPAI GACNNNNGTC 1 cut(s) 917
BstPI GGTNACC 1 cut(s) 575
BstSCI CCNGG 5 cut(s) 77, 483, 579, 588, 1633
BstSFI CTRYAG 2 cut(s) 187, 1500
BstSLI GKGCMC 1 cut(s) 1690
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 7 cut(s) 201, 271, 274, 363, 649, 1200, 1404
BstV2I GAAGAC 2 cut(s) 54, 1223
BstX2I RGATCY 4 cut(s) 115, 255, 805, 1506
BstYI RGATCY 4 cut(s) 115, 255, 805, 1506
BstZI CGGCCG 1 cut(s) 213
Bsu15I ATCGAT 1 cut(s) 69
Bsu36I CCTNAGG 1 cut(s) 1545
BsuI GTATCC 1 cut(s) 1575
BsuRI GGCC 4 cut(s) 215, 480, 1068, 1146
BsuTUI ATCGAT 1 cut(s) 69
BtgI CCRYGG 1 cut(s) 1374
BtsCI GGATG 2 cut(s) 355, 361
BtsIMutI CAGTG 6 cut(s) 610, 623, 664, 758, 1002, 1688
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 4 cut(s) 621, 1205, 1209, 1653
CaiI CAGNNNCTG 1 cut(s) 1398
CfoI GCGC 2 cut(s) 194, 1213
Cfr13I GGNCC 5 cut(s) 22, 81, 479, 1161, 1631
ClaI ATCGAT 1 cut(s) 69
CpoI CGGWCCG 1 cut(s) 22
CseI GACGC 2 cut(s) 434, 907
CsiI ACCWGGT 2 cut(s) 579, 1633
Csp6I GTAC 5 cut(s) 819, 963, 1278, 1326, 1485
CspI CGGWCCG 1 cut(s) 22
CviAII CATG 8 cut(s) 1095, 1139, 1292, 1340, 1375, 1479, 1594, 1671
CviQI GTAC 5 cut(s) 819, 963, 1278, 1326, 1485
DdeI CTNAG 7 cut(s) 387, 794, 1196, 1281, 1322, 1545, 1659
DpnI GATC 9 cut(s) 117, 164, 234, 257, 784, 807, 897, 1257, 1508
DpnII GATC 9 cut(s) 115, 162, 232, 255, 782, 805, 895, 1255, 1506
DraIII CACNNNGTG 1 cut(s) 270
DrdI GACNNNNNNGTC 1 cut(s) 584
DseDI GACNNNNNNGTC 1 cut(s) 584
EaeI YGGCCR 2 cut(s) 213, 1066
EagI CGGCCG 1 cut(s) 213
Ecl136II GAGCTC 2 cut(s) 386, 472
EclXI CGGCCG 1 cut(s) 213
Eco130I CCWWGG 2 cut(s) 1063, 1374
Eco24I GRGCYC 2 cut(s) 388, 474
Eco31I GGTCTC 2 cut(s) 131, 306
Eco47I GGWCC 4 cut(s) 22, 81, 1161, 1631
Eco47III AGCGCT 1 cut(s) 1212
Eco52I CGGCCG 1 cut(s) 213
Eco53kI GAGCTC 2 cut(s) 386, 472
Eco57I CTGAAG 1 cut(s) 699
Eco81I CCTNAGG 1 cut(s) 1545
Eco91I GGTNACC 1 cut(s) 575
EcoICRI GAGCTC 2 cut(s) 386, 472
EcoO65I GGTNACC 1 cut(s) 575
EcoRI GAATTC 1 cut(s) 1529
EcoRII CCWGG 5 cut(s) 77, 483, 579, 588, 1633
EcoT14I CCWWGG 2 cut(s) 1063, 1374
EcoT38I GRGCYC 2 cut(s) 388, 474
ErhI CCWWGG 2 cut(s) 1063, 1374
FaeI CATG 8 cut(s) 1098, 1142, 1295, 1343, 1378, 1482, 1597, 1674
FalI AAGNNNNNCTT 2 cut(s) 672, 704
FaqI GGGAC 1 cut(s) 145
FatI CATG 8 cut(s) 1094, 1138, 1291, 1339, 1374, 1478, 1593, 1670
FauI CCCGC 2 cut(s) 153, 1576
FblI GTMKAC 1 cut(s) 1077
FokI GGATG 2 cut(s) 362, 368
FriOI GRGCYC 2 cut(s) 388, 474
FspBI CTAG 6 cut(s) 335, 380, 1049, 1148, 1272, 1652
GlaI GCGC 2 cut(s) 193, 1212
GsaI CCCAGC 1 cut(s) 153
GsuI CTGGAG 2 cut(s) 184, 312
HaeII RGCGCY 2 cut(s) 195, 1214
HaeIII GGCC 4 cut(s) 215, 480, 1068, 1146
HapII CCGG 2 cut(s) 20, 1086
HgaI GACGC 2 cut(s) 434, 907
HhaI GCGC 2 cut(s) 194, 1213
Hin1I GRCGYC 1 cut(s) 918
Hin1II CATG 8 cut(s) 1098, 1142, 1295, 1343, 1378, 1482, 1597, 1674
Hin6I GCGC 2 cut(s) 192, 1211
HinP1I GCGC 2 cut(s) 192, 1211
HincII GTYRAC 1 cut(s) 1609
HindII GTYRAC 1 cut(s) 1609
HinfI GANTC 7 cut(s) 14, 110, 175, 401, 842, 1447, 1665
HpaII CCGG 2 cut(s) 20, 1086
HphI GGTGA 3 cut(s) 377, 587, 1649
Hpy166II GTNNAC 5 cut(s) 821, 1078, 1485, 1609, 1688
Hpy188I TCNGA 9 cut(s) 115, 174, 232, 400, 816, 1092, 1165, 1255, 1284
Hpy188III TCNNGA 6 cut(s) 181, 422, 786, 902, 1523, 1629
Hpy8I GTNNAC 5 cut(s) 821, 1078, 1485, 1609, 1688
Hpy99I CGWCG 2 cut(s) 107, 110
HpyAV CCTTC 2 cut(s) 243, 1108
HpyCH4III ACNGT 4 cut(s) 318, 659, 932, 1075
HpyCH4IV ACGT 1 cut(s) 441
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 565, 650, 659, 1143, 1213, 1272
HpyF3I CTNAG 7 cut(s) 387, 794, 1196, 1281, 1322, 1545, 1659
HpySE526I ACGT 1 cut(s) 441
Hsp92I GRCGYC 1 cut(s) 918
Hsp92II CATG 8 cut(s) 1098, 1142, 1295, 1343, 1378, 1482, 1597, 1674
HspAI GCGC 2 cut(s) 192, 1211
Kzo9I GATC 9 cut(s) 115, 162, 232, 255, 782, 805, 895, 1255, 1506
LmnI GCTCC 2 cut(s) 383, 1178
Lsp1109I GCAGC 7 cut(s) 201, 271, 274, 363, 649, 1200, 1404
LweI GCATC 3 cut(s) 358, 1124, 1630
MabI ACCWGGT 2 cut(s) 579, 1633
MaeI CTAG 6 cut(s) 335, 380, 1049, 1148, 1272, 1652
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 7 cut(s) 208, 575, 902, 937, 1044, 1335, 1360
MalI GATC 9 cut(s) 117, 164, 234, 257, 784, 807, 897, 1257, 1508
MbiI CCGCTC 1 cut(s) 86
MboI GATC 9 cut(s) 115, 162, 232, 255, 782, 805, 895, 1255, 1506
MfeI CAATTG 2 cut(s) 710, 1602
MflI RGATCY 4 cut(s) 115, 255, 805, 1506
MhlI GDGCHC 3 cut(s) 388, 474, 1690
MlsI TGGCCA 1 cut(s) 1068
MluNI TGGCCA 1 cut(s) 1068
MlyI GAGTC 2 cut(s) 8, 1441
MmeI TCCRAC 1 cut(s) 327
Mox20I TGGCCA 1 cut(s) 1068
MroXI GAANNNNTTC 3 cut(s) 1118, 1308, 1422
MscI TGGCCA 1 cut(s) 1068
MseI TTAA 3 cut(s) 1058, 1317, 1702
MslI CAYNNNNRTG 2 cut(s) 279, 1693
Msp20I TGGCCA 1 cut(s) 1068
MspA1I CMGCKG 2 cut(s) 153, 204
MspI CCGG 2 cut(s) 20, 1086
MspR9I CCNGG 5 cut(s) 79, 485, 581, 590, 1635
MunI CAATTG 2 cut(s) 710, 1602
MvaI CCWGG 5 cut(s) 79, 485, 581, 590, 1635
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 7 cut(s) 83, 565, 650, 659, 1143, 1213, 1272
NcoI CCATGG 1 cut(s) 1374
NdeII GATC 9 cut(s) 115, 162, 232, 255, 782, 805, 895, 1255, 1506
NheI GCTAGC 1 cut(s) 1651
NlaIII CATG 8 cut(s) 1098, 1142, 1295, 1343, 1378, 1482, 1597, 1674
NlaIV GGNNCC 6 cut(s) 117, 272, 481, 550, 723, 1121
NmuCI GTSAC 4 cut(s) 575, 902, 1044, 1335
NspI RCATGY 2 cut(s) 1098, 1343
OliI CACNNNNGTG 1 cut(s) 1693
PcsI WCGNNNNNNNCGW 1 cut(s) 310
PdmI GAANNNNTTC 3 cut(s) 1118, 1308, 1422
PfeI GAWTC 5 cut(s) 110, 175, 401, 842, 1665
PleI GAGTC 2 cut(s) 8, 1441
PpsI GAGTC 2 cut(s) 8, 1441
PshAI GACNNNNGTC 1 cut(s) 917
Psp124BI GAGCTC 2 cut(s) 388, 474
Psp1406I AACGTT 1 cut(s) 441
Psp6I CCWGG 5 cut(s) 77, 483, 579, 588, 1633
PspEI GGTNACC 1 cut(s) 575
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 5 cut(s) 77, 483, 579, 588, 1633
PspN4I GGNNCC 6 cut(s) 117, 272, 481, 550, 723, 1121
PspPI GGNCC 5 cut(s) 22, 81, 479, 1161, 1631
PstI CTGCAG 1 cut(s) 191
PstNI CAGNNNCTG 1 cut(s) 1398
PsuI RGATCY 4 cut(s) 115, 255, 805, 1506
PvuII CAGCTG 1 cut(s) 153
RsaI GTAC 5 cut(s) 820, 964, 1279, 1327, 1486
RsaNI GTAC 5 cut(s) 819, 963, 1278, 1326, 1485
RseI CAYNNNNRTG 2 cut(s) 279, 1693
Rsr2I CGGWCCG 1 cut(s) 22
RsrII CGGWCCG 1 cut(s) 22
SacI GAGCTC 2 cut(s) 388, 474
SaqAI TTAA 3 cut(s) 1058, 1317, 1702
Sau3AI GATC 9 cut(s) 115, 162, 232, 255, 782, 805, 895, 1255, 1506
Sau96I GGNCC 5 cut(s) 22, 81, 479, 1161, 1631
SchI GAGTC 2 cut(s) 8, 1441
ScrFI CCNGG 5 cut(s) 79, 485, 581, 590, 1635
SduI GDGCHC 3 cut(s) 388, 474, 1690
SexAI ACCWGGT 2 cut(s) 579, 1633
SfaNI GCATC 3 cut(s) 358, 1124, 1630
SfcI CTRYAG 2 cut(s) 187, 1500
SinI GGWCC 4 cut(s) 22, 81, 1161, 1631
SmiMI CAYNNNNRTG 2 cut(s) 279, 1693
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
SspI AATATT 1 cut(s) 1040
SspMI CTAG 6 cut(s) 335, 380, 1049, 1148, 1272, 1652
SstI GAGCTC 2 cut(s) 388, 474
StyD4I CCNGG 5 cut(s) 77, 483, 579, 588, 1633
StyI CCWWGG 2 cut(s) 1063, 1374
TaaI ACNGT 4 cut(s) 318, 659, 932, 1075
TaiI ACGT 1 cut(s) 444
TaqI TCGA 5 cut(s) 12, 69, 102, 313, 493
TatI WGTACW 4 cut(s) 818, 1277, 1325, 1484
TauI GCSGC 3 cut(s) 207, 218, 774
TfiI GAWTC 5 cut(s) 110, 175, 401, 842, 1665
Tru1I TTAA 3 cut(s) 1058, 1317, 1702
Tru9I TTAA 3 cut(s) 1058, 1317, 1702
TscAI CASTG 6 cut(s) 610, 630, 664, 765, 1009, 1695
TseFI GTSAC 4 cut(s) 575, 902, 1044, 1335
TseI GCWGC 7 cut(s) 189, 284, 287, 376, 662, 1213, 1392
Tsp45I GTSAC 4 cut(s) 575, 902, 1044, 1335
TspGWI ACGGA 2 cut(s) 138, 149
TspRI CASTG 6 cut(s) 610, 630, 664, 765, 1009, 1695
VneI GTGCAC 1 cut(s) 1686
VpaK11BI GGWCC 4 cut(s) 22, 81, 1161, 1631
XapI RAATTY 3 cut(s) 62, 692, 1529
XceI RCATGY 2 cut(s) 1098, 1343
XmiI GTMKAC 1 cut(s) 1077
XmnI GAANNNNTTC 3 cut(s) 1118, 1308, 1422
XspI CTAG 6 cut(s) 335, 380, 1049, 1148, 1272, 1652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.