RchiOBHm_Chr4g0429241

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0011332
Physical Location & Seq
Reverse (-)
54058333 .. 54064608
6276 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39807

Sequence Viewer

Length: 2490 bp
ATGCTCAGACTGATTTCTCAAAAAACGTTTCAGTGTGCGAATTTCACTACCCATTGCAAACCCAACTCTTGCTCAAGCTTTCAACGATGTGGGTACACTGCCCAATTGACCCAACACTTGACGTGCCACCCAGAGCTTCCCAACCCAGAATTCGACTCTTACGCCTACGCAACTACACTCCAGCATCACATCCAAACCGGCGACTTTACTTCAGGAACGGCTCTTCACTGCGATATTGTGAAGAAAGGCGGATGCTTGGACCTGTTCGCTTACAACATTCTCGCCAACATGTACGTGAAAGCGGGTCTAGTCAGTGATGCAGTCAAGGTGTTCGACGAAATGCCCCACAGAAATGTCATTTCGTTCGTCACGTTAATCCAGGGGTTTTCGGAGTCTCAACGCTTCGTCGAGGCTGTAGAGTTGTTCAGTAGGTTGCATAGAGAAGGCCAGGAGCTGAACCCGTTTGTTTTCACTACCGTCTTGAAGTTGCTTGTGAGGATGGGATTGGCTGAGCTAGCTTGGACTGTCCATGCTTGTATTTATAAGCTCGGACATGGAAACAATGCCTTTGTTGGCACTGCTCTCATTGATGCATACTCTGTTTGCGGCGACGTGGATATTACTAGGGATGTTTTCGATGGGATTGTTTGTAAGGATATGGTAGCTTGGTCTGGGATGGTAGGGTGCTATGCTGAGAATGGCGGTTTTGAAGAAGCGGTTGAGCTCTTCTGTCAAATGAGGGTGATTGGATTCAGGCCTAATAATTATACTTTCAGCGGTGTGCTTAAGGCCTGTCTCAGGTTAAAGGCACTGGATCACGGCAAGTGTGTTCATGGATGTGTGATAAAATCATGTTTTGAAAAGGATCTATATGTTGGTATTTCATTGCTCGATTTATACACCACATGTGGAGATGTTGATGATGCTAGGAGGGTATTTCAAGATATGCCTAAAAATGAAGTGATTCCTTGGACTCTCATGGTTTCACGGTATGCTCAGAGTGACCACTGTGAAGAGGCGCTGGACATGTTCTGTCGGATGAGGCAAGCTTTTGTCGCTCCCAATCAGTTTACGTATGCTAGTGTGCTGCAAGCTTGTGCAACCATGGAGAGTTTAGTTTTGGGGAAGCAAGTACATTGTCAAGTAGTCAAGGTTGGTCTTGACTCAGATATCTATGTTTTAAATGCCCTCATGGATTTCTATGCTAAATGTGGACAAATAGAGAACTCAATGGACCTGTTTGTGGAATCATCGAACAGAAATGATGTATCTTGGAACACAATGATTGTTGGTTATGTCCAGTTAGGTGATGGGGAGAAGGCGTTGACTTTGTTTTCAGATATGCTTAGATGCCAAGTGCAGGTCACAGAAGTGACGTACTCTAGTGCTCTCCGTGCTTCCGCTAGCATAGCAGCGTTGGAGCCAGGACTTCAGATTCATTCAGTGACGATTAAAACCATATTTCACGAGGACACTGTAGTTGGTAATTCTTTGATAGACATGTATGCCAAGTGTGGGAGCATCAAAGATGCCCGCTTGGTGTTCGATAAGTTGAAGCAACGAGATGAAGTTTCATGGAATGCAATGATCTCAGGATATTCTTTGCATGGTCTTGGTAAGGAGGCTCTTAAACTTTTTGAAATGATGCAGGAAACAAATTGCAAACCAAACAAGTTAACCTTTGTTGGTGTCCTATCGGCATGTAGCCAAGCAGGACTGTTAGACCAAGGAAATGCTTATTTTAATTCTATGGTAGAAGACTACTACATTGAACCATGCATGGAGCACTATACTTGTATGGTCTGGCTACTAGGGAAATCAGGCCAGCTTGGTAAGGCTGTGAAGTTGATTGGCGAAATCCCATTTGAACCTAGTGCTATGGTATGGCGTGCTTTGCTCGGAGCCTGTGTTATCCATAATGACGTTGAGCTTGGAAGGATTTGTGCCCAGCATGTGCTTGAAATGGATCCCCAAGATGAGGCTACGCATGTGCTGTTGTCAAACTTATATGCCACTGCAAAGAGGTGGGATAATGTGGCTTCTGTTAGAAAAAACATGAAAAGGAAAGGAGTAAAGAAGGAGCCAGGCCTAAGTTGGATTGAGAACCAGGGCACAGTTCATTATTTCACTGTGGGTGATACTTCACATCCTGATCTGAGACTGATCAATGGGATGCTGGAATGGTTGAAAATGAGAACCTTGAAGGCAGGTCATGTTCCCAATTGTAATGTTGTTATGCTTGATGTTGAGGATGATGAAAAGGAGCGCCTCTTGTGGGTACACAGTGAAAGATTAGCTCTAGCACTTGCGCTGGTTAGAACTCCATCTGGGAGCCCCATTCGTATCATTAAAAATCTTCGGATATGTGTAGACTGCCATGCCACGATGAAGTTAATATCAAAGGTCGTGCAGCGTGAAATAATTGTTAGAGATATGAATCGGTTCCATCACTTTAAGGGTGGCATTTGCTCCTGTGACGATTACTGGTGA

Protein Analysis

829

Amino Acids

92.97

Weight (kDa)

6.17

Isoelectric Point (pI)

32.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 82 - 114 7.6e-06 PPR repeat
PPR PF01535 90 - 118 8.7e-06 PPR repeat
PPR_2 PF13041 219 - 266 2.8e-09 PPR repeat family
PPR_2 PF13041 323 - 367 1.7e-06 PPR repeat family
PPR_2 PF13041 421 - 466 1.7e-10 PPR repeat family
PPR PF01535 423 - 452 1.7e-07 PPR repeat
PPR_2 PF13041 522 - 569 7e-12 PPR repeat family
PPR PF01535 524 - 553 1.8e-08 PPR repeat
E_motif PF20431 639 - 701 8e-23 E motif
DYW_deaminase PF14432 737 - 829 1.3e-41 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011332)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13230
fragaria_vesca FvH4_4g22970 FvH4_4g22970
malus_domestica MD13G1127800.v1.1
prunus_persica Prupe.1G218200_v2.0.a1
pyrus_communis pycom13g11070 pycom16g10860 pycom16g10870
rosa_chinensis RchiOBHm_Chr4g0429241
rosa_laevigata RLG00000007086
rosa_multiflora Rmu_sc0000054.1_g000027
rosa_roxburghii Rroxscaffold_5G00370870
rosa_rugosa Rorug04G0234000 Rorug04G0234100 Rorug04G0234200 Rorug04G0234300 Rorug04G0234400
rosa_samantha Rh4AG290100 Rh4BG295800
rosa_wichuraiana Rw4G025120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 543
Acc36I ACCTGC 2 cut(s) 1351, 2198
AccI GTMKAC 1 cut(s) 2370
AciI CCGC 8 cut(s) 249, 302, 606, 702, 716, 777, 1401, 1534
AclI AACGTT 1 cut(s) 26
AclWI GGATC 4 cut(s) 822, 873, 1961, 1974
AcsI RAATTY 2 cut(s) 40, 149
AcuI CTGAAG 2 cut(s) 195, 1415
AfaI GTAC 5 cut(s) 95, 293, 1134, 1379, 2280
AfiI CCNNNNNNNGG 6 cut(s) 798, 1243, 1360, 1515, 1978, 2275
AflII CTTAAG 1 cut(s) 785
AflIII ACRYGT 4 cut(s) 288, 905, 1026, 1500
AjiI CACGTC 2 cut(s) 123, 613
AjnI CCWGG 5 cut(s) 378, 447, 1423, 2083, 2106
AjuI GAANNNNNNNTTGG 2 cut(s) 134, 166
AleI CACNNNNGTG 1 cut(s) 1370
Alw21I GWGCWC 3 cut(s) 726, 1390, 1788
Alw26I GTCTC 3 cut(s) 399, 800, 2152
AlwI GGATC 4 cut(s) 822, 873, 1961, 1974
AlwNI CAGNNNCTG 1 cut(s) 454
AoxI GGCC 5 cut(s) 445, 755, 789, 1822, 2086
ApeKI GCWGC 3 cut(s) 1087, 1412, 2410
ApoI RAATTY 2 cut(s) 40, 149
Asp700I GAANNNNTTC 2 cut(s) 963, 2441
AspLEI GCGC 3 cut(s) 1021, 2268, 2311
AspS9I GGNCC 2 cut(s) 259, 1234
AsuHPI GGTGA 3 cut(s) 754, 1319, 2147
AsuNHI GCTAGC 2 cut(s) 514, 1403
AvaII GGWCC 2 cut(s) 259, 1234
BaeGI GKGCMC 2 cut(s) 1948, 2114
BamHI GGATCC 1 cut(s) 1966
BanII GRGCYC 2 cut(s) 726, 2336
BauI CACGAG 1 cut(s) 1466
BbsI GAAGAC 1 cut(s) 1764
Bbv12I GWGCWC 3 cut(s) 726, 1390, 1788
BbvI GCAGC 3 cut(s) 1074, 1424, 2422
BccI CCATC 6 cut(s) 493, 632, 670, 1304, 2332, 2454
BceAI ACGGC 2 cut(s) 234, 835
BciT130I CCWGG 5 cut(s) 380, 449, 1425, 2085, 2108
BclI TGATCA 1 cut(s) 2163
BcoDI GTCTC 3 cut(s) 399, 800, 2152
BfmI CTRYAG 2 cut(s) 414, 1476
BfoI RGCGCY 2 cut(s) 1022, 2269
BfrI CTTAAG 1 cut(s) 785
BfuAI ACCTGC 2 cut(s) 1351, 2198
BisI GCNGC 4 cut(s) 607, 1088, 1413, 2411
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 4 cut(s) 608, 1089, 1414, 2412
Bme1390I CCNGG 5 cut(s) 380, 449, 1425, 2085, 2108
Bme18I GGWCC 2 cut(s) 259, 1234
BmgBI CACGTC 2 cut(s) 123, 613
BmgT120I GGNCC 2 cut(s) 259, 1234
BmiI GGNNCC 6 cut(s) 1422, 1903, 1968, 2082, 2333, 2444
BmrFI CCNGG 5 cut(s) 380, 449, 1425, 2085, 2108
BmtI GCTAGC 2 cut(s) 518, 1407
BpiI GAAGAC 1 cut(s) 1764
BpmI CTGGAG 1 cut(s) 164
Bpu1102I GCTNAGC 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 58
BsaAI YACGTR 2 cut(s) 295, 1074
BsaBI GATNNNNATC 1 cut(s) 2436
BsaJI CCNNGG 5 cut(s) 379, 968, 1104, 1726, 2107
BsaXI ACNNNNNCTCC 4 cut(s) 1307, 1337, 1893, 1923
Bsc4I CCNNNNNNNGG 6 cut(s) 798, 1243, 1360, 1515, 1978, 2275
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 3 cut(s) 816, 1300, 2489
Bse3DI GCAATG 3 cut(s) 52, 884, 1590
Bse8I GATNNNNATC 1 cut(s) 2436
BseBI CCWGG 5 cut(s) 380, 449, 1425, 2085, 2108
BseDI CCNNGG 5 cut(s) 379, 968, 1104, 1726, 2107
BseJI GATNNNNATC 1 cut(s) 2436
BseLI CCNNNNNNNGG 6 cut(s) 798, 1243, 1360, 1515, 1978, 2275
BseMI GCAATG 3 cut(s) 52, 884, 1590
BseMII CTCAG 8 cut(s) 19, 501, 684, 811, 1010, 1179, 1605, 2147
BseNI ACTGG 3 cut(s) 816, 1300, 2489
BseSI GKGCMC 2 cut(s) 1948, 2114
BseXI GCAGC 3 cut(s) 1074, 1424, 2422
BseYI CCCAGC 1 cut(s) 1947
BsgI GTGCAG 2 cut(s) 1379, 2429
BshFI GGCC 5 cut(s) 447, 757, 791, 1824, 2088
BsiHKAI GWGCWC 3 cut(s) 726, 1390, 1788
BsiSI CCGG 1 cut(s) 198
BslI CCNNNNNNNGG 6 cut(s) 798, 1243, 1360, 1515, 1978, 2275
BsmAI GTCTC 3 cut(s) 399, 800, 2152
BsmI GAATGC 1 cut(s) 1585
BsnI GGCC 5 cut(s) 447, 757, 791, 1824, 2088
Bsp1286I GDGCHC 6 cut(s) 726, 1390, 1788, 1948, 2114, 2336
Bsp143I GATC 6 cut(s) 814, 865, 1587, 1966, 2152, 2163
Bsp1720I GCTNAGC 1 cut(s) 510
Bsp19I CCATGG 1 cut(s) 1104
BspACI CCGC 8 cut(s) 249, 302, 606, 702, 716, 777, 1401, 1534
BspANI GGCC 5 cut(s) 447, 757, 791, 1824, 2088
BspCNI CTCAG 8 cut(s) 18, 502, 685, 810, 1009, 1178, 1604, 2148
BspLI GGNNCC 6 cut(s) 1422, 1903, 1968, 2082, 2333, 2444
BspMI ACCTGC 2 cut(s) 1351, 2198
BspOI GCTAGC 2 cut(s) 518, 1407
BspPI GGATC 4 cut(s) 822, 873, 1961, 1974
BspQI GCTCTTC 2 cut(s) 228, 731
BspTI CTTAAG 1 cut(s) 785
BsrDI GCAATG 3 cut(s) 52, 884, 1590
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 3 cut(s) 816, 1300, 2489
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 5 cut(s) 379, 968, 1104, 1726, 2107
BssMI GATC 6 cut(s) 814, 865, 1587, 1966, 2152, 2163
BssSI CACGAG 1 cut(s) 1466
BssT1I CCWWGG 3 cut(s) 968, 1104, 1726
Bst2BI CACGAG 1 cut(s) 1466
Bst2UI CCWGG 5 cut(s) 380, 449, 1425, 2085, 2108
Bst4CI ACNGT 9 cut(s) 478, 526, 990, 1010, 1477, 1719, 2116, 2131, 2285
Bst6I CTCTTC 3 cut(s) 228, 731, 1008
BstAFI CTTAAG 1 cut(s) 785
BstBAI YACGTR 2 cut(s) 295, 1074
BstC8I GCNNGC 7 cut(s) 516, 1047, 1092, 1405, 1534, 1826, 1890
BstDSI CCRYGG 1 cut(s) 1104
BstENI CCTNNNNNAGG 1 cut(s) 796
BstH2I RGCGCY 2 cut(s) 1022, 2269
BstHHI GCGC 3 cut(s) 1021, 2268, 2311
BstKTI GATC 6 cut(s) 817, 868, 1590, 1969, 2155, 2166
BstMAI GTCTC 3 cut(s) 399, 800, 2152
BstMBI GATC 6 cut(s) 814, 865, 1587, 1966, 2152, 2163
BstMWI GCNNNNNNNGC 5 cut(s) 515, 1055, 1394, 1409, 1894
BstNI CCWGG 5 cut(s) 380, 449, 1425, 2085, 2108
BstNSI RCATGY 7 cut(s) 292, 909, 1030, 1504, 1704, 1955, 1991
BstSCI CCNGG 5 cut(s) 378, 447, 1423, 2083, 2106
BstSFI CTRYAG 2 cut(s) 414, 1476
BstSLI GKGCMC 2 cut(s) 1948, 2114
BstSNI TACGTA 1 cut(s) 1074
BstV1I GCAGC 3 cut(s) 1074, 1424, 2422
BstV2I GAAGAC 1 cut(s) 1764
BstX2I RGATCY 2 cut(s) 865, 1966
BstYI RGATCY 2 cut(s) 865, 1966
BsuRI GGCC 5 cut(s) 447, 757, 791, 1824, 2088
BtgI CCRYGG 1 cut(s) 1104
BtrI CACGTC 2 cut(s) 123, 613
BtsI GCAGTG 4 cut(s) 96, 226, 576, 2013
BveI ACCTGC 2 cut(s) 1351, 2198
Cac8I GCNNGC 7 cut(s) 516, 1047, 1092, 1405, 1534, 1826, 1890
CaiI CAGNNNCTG 1 cut(s) 454
CfoI GCGC 3 cut(s) 1021, 2268, 2311
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 2 cut(s) 259, 1234
Csp6I GTAC 5 cut(s) 94, 292, 1133, 1378, 2279
CviQI GTAC 5 cut(s) 94, 292, 1133, 1378, 2279
DpnI GATC 6 cut(s) 816, 867, 1589, 1968, 2154, 2165
DpnII GATC 6 cut(s) 814, 865, 1587, 1966, 2152, 2163
DraI TTTAAA 1 cut(s) 1182
Eam1104I CTCTTC 3 cut(s) 228, 731, 1008
EarI CTCTTC 3 cut(s) 228, 731, 1008
EciI GGCGGA 1 cut(s) 264
Ecl136II GAGCTC 1 cut(s) 724
Eco105I TACGTA 1 cut(s) 1074
Eco130I CCWWGG 3 cut(s) 968, 1104, 1726
Eco147I AGGCCT 3 cut(s) 757, 791, 2088
Eco24I GRGCYC 2 cut(s) 726, 2336
Eco32I GATATC 1 cut(s) 1171
Eco47I GGWCC 2 cut(s) 259, 1234
Eco53kI GAGCTC 1 cut(s) 724
Eco57I CTGAAG 2 cut(s) 195, 1415
EcoICRI GAGCTC 1 cut(s) 724
EcoNI CCTNNNNNAGG 1 cut(s) 796
EcoRI GAATTC 1 cut(s) 149
EcoRII CCWGG 5 cut(s) 378, 447, 1423, 2083, 2106
EcoRV GATATC 1 cut(s) 1171
EcoT14I CCWWGG 3 cut(s) 968, 1104, 1726
EcoT22I ATGCAT 2 cut(s) 595, 1781
EcoT38I GRGCYC 2 cut(s) 726, 2336
ErhI CCWWGG 3 cut(s) 968, 1104, 1726
FalI AAGNNNNNCTT 2 cut(s) 1664, 1696
FauI CCCGC 2 cut(s) 295, 1541
FbaI TGATCA 1 cut(s) 2163
FblI GTMKAC 1 cut(s) 2370
Fnu4HI GCNGC 4 cut(s) 607, 1088, 1413, 2411
FriOI GRGCYC 2 cut(s) 726, 2336
Fsp4HI GCNGC 4 cut(s) 607, 1088, 1413, 2411
GlaI GCGC 3 cut(s) 1020, 2267, 2310
GluI GCNGC 4 cut(s) 607, 1088, 1413, 2411
GsaI CCCAGC 1 cut(s) 1951
GsuI CTGGAG 1 cut(s) 164
HaeII RGCGCY 2 cut(s) 1022, 2269
HaeIII GGCC 5 cut(s) 447, 757, 791, 1824, 2088
HapII CCGG 1 cut(s) 198
HhaI GCGC 3 cut(s) 1021, 2268, 2311
Hin6I GCGC 3 cut(s) 1019, 2266, 2309
HinP1I GCGC 3 cut(s) 1019, 2266, 2309
HincII GTYRAC 2 cut(s) 1326, 1677
HindII GTYRAC 2 cut(s) 1326, 1677
HindIII AAGCTT 3 cut(s) 76, 1047, 1092
HinfI GANTC 9 cut(s) 155, 392, 750, 964, 973, 1163, 1247, 1435, 2437
HpaI GTTAAC 1 cut(s) 1677
HpaII CCGG 1 cut(s) 198
HphI GGTGA 3 cut(s) 754, 1319, 2147
Hpy166II GTNNAC 7 cut(s) 96, 1071, 1214, 1326, 1677, 2281, 2371
Hpy188III TCNNGA 7 cut(s) 213, 481, 941, 1160, 1466, 1593, 2150
Hpy8I GTNNAC 7 cut(s) 96, 1071, 1214, 1326, 1677, 2281, 2371
Hpy99I CGWCG 3 cut(s) 338, 410, 614
HpyAV CCTTC 5 cut(s) 437, 1312, 1929, 2071, 2197
HpyCH4III ACNGT 9 cut(s) 478, 526, 990, 1010, 1477, 1719, 2116, 2131, 2285
HpyCH4IV ACGT 8 cut(s) 26, 122, 294, 371, 612, 1073, 1376, 1923
HpyF10VI GCNNNNNNNGC 5 cut(s) 515, 1055, 1394, 1409, 1894
HpySE526I ACGT 8 cut(s) 26, 122, 294, 371, 612, 1073, 1376, 1923
HspAI GCGC 3 cut(s) 1019, 2266, 2309
Ksp22I TGATCA 1 cut(s) 2163
KspAI GTTAAC 1 cut(s) 1677
Kzo9I GATC 6 cut(s) 814, 865, 1587, 1966, 2152, 2163
LguI GCTCTTC 2 cut(s) 228, 731
Lsp1109I GCAGC 3 cut(s) 1074, 1424, 2422
MaeII ACGT 8 cut(s) 26, 122, 294, 371, 612, 1073, 1376, 1923
MaeIII GTNAC 6 cut(s) 367, 1001, 1363, 1372, 1444, 2474
MalI GATC 6 cut(s) 816, 867, 1589, 1968, 2154, 2165
MboI GATC 6 cut(s) 814, 865, 1587, 1966, 2152, 2163
MboII GAAGA 7 cut(s) 215, 253, 718, 722, 1025, 1769, 2348
MfeI CAATTG 2 cut(s) 104, 2221
MflI RGATCY 2 cut(s) 865, 1966
MhlI GDGCHC 6 cut(s) 726, 1390, 1788, 1948, 2114, 2336
MluCI AATT 9 cut(s) 40, 104, 149, 763, 1486, 1657, 1744, 2221, 2421
MlyI GAGTC 4 cut(s) 149, 401, 967, 1157
MmeI TCCRAC 3 cut(s) 1016, 1398, 2075
Mph1103I ATGCAT 2 cut(s) 595, 1781
MroXI GAANNNNTTC 2 cut(s) 963, 2441
MslI CAYNNNNRTG 4 cut(s) 293, 351, 837, 1370
MspA1I CMGCKG 1 cut(s) 777
MspCI CTTAAG 1 cut(s) 785
MspI CCGG 1 cut(s) 198
MspR9I CCNGG 5 cut(s) 380, 449, 1425, 2085, 2108
MunI CAATTG 2 cut(s) 104, 2221
Mva1269I GAATGC 1 cut(s) 1585
MvaI CCWGG 5 cut(s) 380, 449, 1425, 2085, 2108
MwoI GCNNNNNNNGC 5 cut(s) 515, 1055, 1394, 1409, 1894
NcoI CCATGG 1 cut(s) 1104
NdeII GATC 6 cut(s) 814, 865, 1587, 1966, 2152, 2163
NheI GCTAGC 2 cut(s) 514, 1403
NlaIV GGNNCC 6 cut(s) 1422, 1903, 1968, 2082, 2333, 2444
NmuCI GTSAC 6 cut(s) 367, 1001, 1363, 1372, 1444, 2474
NsiI ATGCAT 2 cut(s) 595, 1781
NspI RCATGY 7 cut(s) 292, 909, 1030, 1504, 1704, 1955, 1991
OliI CACNNNNGTG 1 cut(s) 1370
PceI AGGCCT 3 cut(s) 757, 791, 2088
PciI ACATGT 4 cut(s) 288, 905, 1026, 1500
PciSI GCTCTTC 2 cut(s) 228, 731
PcsI WCGNNNNNNNCGW 1 cut(s) 368
PctI GAATGC 1 cut(s) 1585
PdmI GAANNNNTTC 2 cut(s) 963, 2441
PfeI GAWTC 5 cut(s) 750, 964, 1247, 1435, 2437
PkrI GCNGC 4 cut(s) 608, 1089, 1414, 2412
PleI GAGTC 4 cut(s) 149, 400, 967, 1157
PpsI GAGTC 4 cut(s) 149, 400, 967, 1157
Ppu21I YACGTR 2 cut(s) 295, 1074
PscI ACATGT 4 cut(s) 288, 905, 1026, 1500
PsiI TTATAA 1 cut(s) 543
Psp124BI GAGCTC 1 cut(s) 726
Psp1406I AACGTT 1 cut(s) 26
Psp6I CCWGG 5 cut(s) 378, 447, 1423, 2083, 2106
PspFI CCCAGC 1 cut(s) 1947
PspGI CCWGG 5 cut(s) 378, 447, 1423, 2083, 2106
PspN4I GGNNCC 6 cut(s) 1422, 1903, 1968, 2082, 2333, 2444
PspPI GGNCC 2 cut(s) 259, 1234
PstNI CAGNNNCTG 1 cut(s) 454
PsuI RGATCY 2 cut(s) 865, 1966
RsaI GTAC 5 cut(s) 95, 293, 1134, 1379, 2280
RsaNI GTAC 5 cut(s) 94, 292, 1133, 1378, 2279
RseI CAYNNNNRTG 4 cut(s) 293, 351, 837, 1370
SacI GAGCTC 1 cut(s) 726
SapI GCTCTTC 2 cut(s) 228, 731
SatI GCNGC 4 cut(s) 607, 1088, 1413, 2411
Sau3AI GATC 6 cut(s) 814, 865, 1587, 1966, 2152, 2163
Sau96I GGNCC 2 cut(s) 259, 1234
SchI GAGTC 4 cut(s) 149, 401, 967, 1157
ScrFI CCNGG 5 cut(s) 380, 449, 1425, 2085, 2108
SduI GDGCHC 6 cut(s) 726, 1390, 1788, 1948, 2114, 2336
SfcI CTRYAG 2 cut(s) 414, 1476
SinI GGWCC 2 cut(s) 259, 1234
SmiMI CAYNNNNRTG 4 cut(s) 293, 351, 837, 1370
SmlI CTYRAG 2 cut(s) 73, 785
SmoI CTYRAG 2 cut(s) 73, 785
SnaBI TACGTA 1 cut(s) 1074
Sse9I AATT 9 cut(s) 40, 104, 149, 763, 1486, 1657, 1744, 2221, 2421
SseBI AGGCCT 3 cut(s) 757, 791, 2088
SsiI CCGC 8 cut(s) 249, 302, 606, 702, 716, 777, 1401, 1534
SstI GAGCTC 1 cut(s) 726
StuI AGGCCT 3 cut(s) 757, 791, 2088
StyD4I CCNGG 5 cut(s) 378, 447, 1423, 2083, 2106
StyI CCWWGG 3 cut(s) 968, 1104, 1726
TaaI ACNGT 9 cut(s) 478, 526, 990, 1010, 1477, 1719, 2116, 2131, 2285
TaiI ACGT 8 cut(s) 29, 125, 297, 374, 615, 1076, 1379, 1926
TaqI TCGA 7 cut(s) 153, 333, 408, 636, 891, 1253, 1545
TasI AATT 9 cut(s) 40, 104, 149, 763, 1486, 1657, 1744, 2221, 2421
TatI WGTACW 1 cut(s) 1132
TauI GCSGC 1 cut(s) 609
TfiI GAWTC 5 cut(s) 750, 964, 1247, 1435, 2437
TseFI GTSAC 6 cut(s) 367, 1001, 1363, 1372, 1444, 2474
TseI GCWGC 3 cut(s) 1087, 1412, 2410
Tsp45I GTSAC 6 cut(s) 367, 1001, 1363, 1372, 1444, 2474
TspGWI ACGGA 1 cut(s) 1382
Vha464I CTTAAG 1 cut(s) 785
VpaK11BI GGWCC 2 cut(s) 259, 1234
XagI CCTNNNNNAGG 1 cut(s) 796
XapI RAATTY 2 cut(s) 40, 149
XceI RCATGY 7 cut(s) 292, 909, 1030, 1504, 1704, 1955, 1991
XcmI CCANNNNNNNNNTGG 2 cut(s) 1307, 2091
XmiI GTMKAC 1 cut(s) 2370
XmnI GAANNNNTTC 2 cut(s) 963, 2441
Zsp2I ATGCAT 2 cut(s) 595, 1781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.