RchiOBHm_Chr4g0432411

Oxysterol-binding protein-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0011201
Physical Location & Seq
Forward (+)
56593039 .. 56598491
5453 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40102

Sequence Viewer

Length: 2451 bp
ATGAACCCGCTGTGCTGCATTGCTCCGGTTTCGATCGATCGGGACCAGGCCGACCCGGTCACGGCGAAGCAGTCCGGTCACTACCAGCTCGGACTCGACGCGGCGGTTCCCGTTCGAAACGTCAGCTACGGCTCGAAGCCGAGCTACTCCGCTCAGGTCTCGTCGGCGGGGACCGAATCGGACAGAGCCTCGTCCGCCGCGGCGCAACGCGAGGTTGAGGAAGCGTTCTGCGAGCCGAGAGATTCGAAGGTGTTCGGCGTCAGTGGAGGCGTCGCCGGAATTCTGTACAAATGGGTGAACTACGGCAAGGGGTGGAGGTCGAGGTGGTTTCTGCTGGAAGACGGCGTGCTTTCGTATTACAAGGTTCATGGACCGGACAAGATTCTGATGAGCCCGGCCAGAGAGAAAGGCGTGACGGTGATCGGAGAGGACTCGTTGAGGTATATGAAGAAGGCTAATTGGAATAGAAATCGGCTTGGCTTGCCGGCGAGGAGGCCGTGCAAGCCTTTCGGCGAAGTGCATTTGAAGGTCTCTTCAATTCGTGCCAGCAAATCAGATGATAAAAGGCTTTCCATATTCACAGGAACTAAAACTCTTCACTTGCGTTGTTTATCTAGAGAGGACAGAGCTGCATGGATTGAGGCATTGCTGGCTGCTAAAGATCTTTTCCCCAGAGTGTTGTCAAGCAATGATTTAGTGCCTTCAGAAGATATAGTTGTTTCAACAGAGAAGTTACGATTAAGATTGGCACAGGAGGGAGTTGGTGAGCCTGTTATCAAAGACTGTGAGTCAATTATGCTTTTAGAAGTCTCAGAGCTGCAAAACAAGTTGAAGGCTCTTCAGAGTAAACATGTTATGTTGTTAGACACATTGAGACAATTGGAGACAGAGAAAATAGAGCTGGAAACTACAGTAGTGGACGAAACAAAGGAGCGTGAGTCATACTGCAGTCAGGGCAACAGGAGACATAGTGATTTTTATTCCGTCATGTCAGAGGGCAGTGGAAGTGATTCTGATGCAGAAAATGAAAGTCAAGACGGAGGAGATGTTGAAACAGATGAAGATGAAGGAACATACTTTGATACTTATGATATTTTGTCTTCAGATGCTTTAAGAAGTGCATCCTACCGGAGTAGAGAAGATGCTTTAAGAAGTGCTTCCTATCGGAGTAGGGATGGTTTGGGAAACGGTGGTGGAGATTCTCTCTTTTCTGATCGATTGCATGCAGTTCAGGAGATCATGACAGTTAAATATCCATCTGTGAAAAGGAGGGATAATTTGCCAGAACCAAAGGAGAAAGATAAGCCTATTGGCTTGTGGTCAATTATCAAGGACAATATTGGAAAGGACTTATCTGGAGTTTGTCTTCCTGTTTATTTTAATGAACCACTCTCTTCATTGCAAAAGTGCTTTGAAGATCTGGAGTATTCCTACTTGGTAGATCGAGCATTGGAATGGGGAAAGCAGGGGAATGACTTGATGAGAATTCTCCACGTGGCAGCTTTTGCTGTATCTGGTTATGCATCAACAGAAGGTCGGCAGTGCAAACCCTTCAATCCTCTCCTGGGGGAGACCTATGAGGCTGATTACCCGGATAAAGGACTTCGTTTCTTTTCTGAAAAGGTGAGTCACCACCCAATGATTGTTGCCTGTCACTGTCAAGGAAGAAATTGGAAATTTTGGGCTGACTCCAACCTTAAAGGCAAGTTTTGGGGGCGTTCTATACAGCTTGATCCTGTGGGTCTGCTTAACTTGCAGTTTGAGGATGGGGAAACATTTGAGTGGAGCAAGGTCACTACTTCCATATACAATATCATACTAGGGAAAATTTACTGCGACCATTATGGCACCATGCGCATCAAGGGAAGTGGAAATTACTCATGTAAGCTAAAATTCAAAGAGCAGTCTATCATAGACCGAAACCCACATCAGGTTCATGGATTTGTTCAAGACAATCGAACTGGAGAGAAAGTAGCAATGCTGATGGGGAAGTGGGATGAGGCAATGTACTATGTTCTAGGAGATCCAACTACAAAACCAAAGGGTTATGATCCAATGACAGAGGCTGTGCTGCTCTGGGAAAGGGAAAACTACGTTACCAAGACAAGATATAATTTCTCTCCTTTTGCAATATCCCTGAATGAATTAACACCTGGACTATTGGAGAAATTGCCTCCAACAGACTCGAGGCTGAGGCCAGATCAAAGACATTTAGAAAATGGTGAATATGAATTGGCAAATGCAGAAAAACTAAGACTTGAACAGCTACAGAGACAGGCAAGGAAGTTGCAAGAGAGAGGCTGGCAGCCAAGATGGTTTCAAAGAGATCAGGACGGTTGTTATAATTACACGGGTGGGTATTGGGAAACTAGGGAGAAAAACAATTGGGATGGAATTCCTGATATTTTTGGCCAGAGTAGTGATACGCCCTCAAGTTCTGTTGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oxysterol_BP PH_11

Protein Analysis

816

Amino Acids

92.47

Weight (kDa)

5.79

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 93 - 219 1.8e-07 PH domain
PH_11 PF15413 94 - 217 1.9e-16 Pleckstrin homology domain
Oxysterol_BP PF01237 439 - 792 7.6e-127 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13170 AT1G13170
fragaria_vesca FvH4_4g25220 FvH4_4g25220
malus_domestica MD13G1105800.v1.1 MD16G1106000.v1.1
prunus_persica Prupe.1G240400_v2.0.a1
pyrus_communis pycom13g09180 pycom16g09010
rosa_chinensis RchiOBHm_Chr4g0432411
rosa_laevigata RLG00000003003 RLG00000006845
rosa_multiflora Rmu_sc0001084.1_g000022
rosa_roxburghii Rroxscaffold_5G00373880
rosa_rugosa Rorug04G0258300 Rorug04G0258400
rosa_samantha Rh4BG321800 Rh4DG317500
rosa_wichuraiana Rw4G027300 Rw7G029610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2343
Acc16I TGCGCA 1 cut(s) 1856
AccB1I GGYRCC 1 cut(s) 1847
AccBSI CCGCTC 1 cut(s) 152
AccII CGCG 3 cut(s) 101, 200, 210
AciI CCGC 8 cut(s) 8, 101, 104, 150, 167, 195, 198, 200
AclWI GGATC 3 cut(s) 1727, 2018, 2045
AcoI YGGCCR 2 cut(s) 396, 2410
AcsI RAATTY 6 cut(s) 279, 1485, 1676, 1827, 1892, 2394
AcuI CTGAAG 3 cut(s) 687, 824, 1086
AcvI CACGTG 1 cut(s) 1495
AcyI GRCGYC 2 cut(s) 258, 270
AfaI GTAC 2 cut(s) 287, 2009
AfiI CCNNNNNNNGG 4 cut(s) 61, 1565, 1598, 1930
AflIII ACRYGT 1 cut(s) 850
AhdI GACNNNNNGTC 1 cut(s) 787
AjnI CCWGG 3 cut(s) 45, 1563, 2152
Alw26I GTCTC 8 cut(s) 163, 535, 814, 868, 878, 958, 1565, 2266
AlwI GGATC 3 cut(s) 1727, 2018, 2045
AlwNI CAGNNNCTG 1 cut(s) 2066
Ama87I CYCGRG 1 cut(s) 2185
AoxI GGCC 5 cut(s) 48, 396, 494, 2195, 2410
ApeKI GCWGC 7 cut(s) 15, 629, 653, 817, 1499, 2071, 2305
ApoI RAATTY 6 cut(s) 279, 1485, 1676, 1827, 1892, 2394
Asp700I GAANNNNTTC 3 cut(s) 251, 1009, 1156
AspLEI GCGC 2 cut(s) 205, 1857
AspS9I GGNCC 3 cut(s) 43, 171, 371
AsuC2I CCSGG 3 cut(s) 56, 395, 1592
AsuHPI GGTGA 6 cut(s) 307, 430, 776, 1622, 1636, 2234
AsuII TTCGAA 2 cut(s) 115, 245
AvaI CYCGRG 1 cut(s) 2185
AvaII GGWCC 3 cut(s) 43, 171, 371
BalI TGGCCA 1 cut(s) 2412
BanI GGYRCC 1 cut(s) 1847
BanII GRGCYC 1 cut(s) 395
BarI GAAGNNNNNNTAC 2 cut(s) 128, 160
BbrPI CACGTG 1 cut(s) 1495
BbsI GAAGAC 3 cut(s) 345, 1092, 1358
BbvCI CCTCAGC 1 cut(s) 2192
BbvI GCAGC 7 cut(s) 2, 616, 640, 804, 1511, 2058, 2317
BccI CCATC 6 cut(s) 1169, 1264, 1760, 1978, 2307, 2384
BceAI ACGGC 5 cut(s) 78, 145, 319, 358, 481
BcgI CGANNNNNNTGC 2 cut(s) 490, 524
BciT130I CCWGG 3 cut(s) 47, 1565, 2154
BcnI CCSGG 3 cut(s) 56, 395, 1592
BcoDI GTCTC 8 cut(s) 163, 535, 814, 868, 878, 958, 1565, 2266
BfaI CTAG 4 cut(s) 615, 1820, 2018, 2370
BfmI CTRYAG 3 cut(s) 909, 946, 2267
BglII AGATCT 2 cut(s) 661, 1417
Bme1390I CCNGG 6 cut(s) 47, 56, 395, 1565, 1592, 2154
Bme18I GGWCC 3 cut(s) 43, 171, 371
BmeRI GACNNNNNGTC 1 cut(s) 787
BmeT110I CYCGRG 1 cut(s) 2185
BmgT120I GGNCC 3 cut(s) 43, 171, 371
BmiI GGNNCC 4 cut(s) 44, 108, 172, 1849
BmrFI CCNGG 6 cut(s) 47, 56, 395, 1565, 1592, 2154
BmsI GCATC 6 cut(s) 1006, 1096, 1130, 1132, 1532, 1866
BpiI GAAGAC 3 cut(s) 345, 1092, 1358
BplI GAGNNNNNCTC 2 cut(s) 1188, 1220
BpmI CTGGAG 3 cut(s) 1377, 1442, 1983
Bpu10I CCTNAGC 2 cut(s) 153, 2192
Bpu14I TTCGAA 2 cut(s) 115, 245
BpuEI CTTGAG 1 cut(s) 2416
BpuMI CCSGG 3 cut(s) 56, 395, 1592
Bsa29I ATCGAT 2 cut(s) 36, 1216
BsaAI YACGTR 1 cut(s) 1495
BsaHI GRCGYC 2 cut(s) 258, 270
BsaI GGTCTC 3 cut(s) 163, 535, 1565
BsaJI CCNNGG 2 cut(s) 198, 1564
BsaWI WCCGGW 4 cut(s) 25, 74, 373, 1128
BsaXI ACNNNNNCTCC 8 cut(s) 923, 953, 955, 985, 1032, 1062, 1956, 1986
Bsc4I CCNNNNNNNGG 4 cut(s) 61, 1565, 1598, 1930
Bse118I RCCGGY 1 cut(s) 484
Bse1I ACTGG 1 cut(s) 1966
Bse3DI GCAATG 6 cut(s) 18, 644, 694, 1397, 1983, 2010
BseBI CCWGG 3 cut(s) 47, 1565, 2154
BseCI ATCGAT 2 cut(s) 36, 1216
BseDI CCNNGG 2 cut(s) 198, 1564
BseGI GGATG 5 cut(s) 1121, 1180, 1771, 2002, 2395
BseLI CCNNNNNNNGG 4 cut(s) 61, 1565, 1598, 1930
BseMI GCAATG 6 cut(s) 18, 644, 694, 1397, 1983, 2010
BseMII CTCAG 3 cut(s) 167, 825, 2183
BseNI ACTGG 1 cut(s) 1966
BseRI GAGGAG 2 cut(s) 505, 1056
BseXI GCAGC 7 cut(s) 2, 616, 640, 804, 1511, 2058, 2317
Bsh1236I CGCG 3 cut(s) 101, 200, 210
Bsh1285I CGRYCG 2 cut(s) 36, 40
BshFI GGCC 5 cut(s) 50, 398, 496, 2197, 2412
BshNI GGYRCC 1 cut(s) 1847
BshVI ATCGAT 2 cut(s) 36, 1216
BsiEI CGRYCG 2 cut(s) 36, 40
BsiHKCI CYCGRG 1 cut(s) 2185
BsiSI CCGG 9 cut(s) 26, 56, 75, 276, 374, 395, 485, 1129, 1592
BslFI GGGAC 2 cut(s) 56, 184
BslI CCNNNNNNNGG 4 cut(s) 61, 1565, 1598, 1930
BsmAI GTCTC 8 cut(s) 163, 535, 814, 868, 878, 958, 1565, 2266
BsmFI GGGAC 2 cut(s) 56, 184
BsnI GGCC 5 cut(s) 50, 398, 496, 2197, 2412
Bso31I GGTCTC 3 cut(s) 163, 535, 1565
BsoBI CYCGRG 1 cut(s) 2185
Bsp119I TTCGAA 2 cut(s) 115, 245
Bsp1286I GDGCHC 1 cut(s) 395
Bsp1407I TGTACA 1 cut(s) 285
BspACI CCGC 8 cut(s) 8, 101, 104, 150, 167, 195, 198, 200
BspANI GGCC 5 cut(s) 50, 398, 496, 2197, 2412
BspCNI CTCAG 3 cut(s) 166, 824, 2184
BspDI ATCGAT 2 cut(s) 36, 1216
BspFNI CGCG 3 cut(s) 101, 200, 210
BspHI TCATGA 1 cut(s) 1239
BspLI GGNNCC 4 cut(s) 44, 108, 172, 1849
BspMAI CTGCAG 1 cut(s) 950
BspPI GGATC 3 cut(s) 1727, 2018, 2045
BspQI GCTCTTC 1 cut(s) 843
BspT104I TTCGAA 2 cut(s) 115, 245
BspT107I GGYRCC 1 cut(s) 1847
BspTNI GGTCTC 3 cut(s) 163, 535, 1565
BsrBI CCGCTC 1 cut(s) 152
BsrDI GCAATG 6 cut(s) 18, 644, 694, 1397, 1983, 2010
BsrFI RCCGGY 1 cut(s) 484
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 1 cut(s) 1966
BssAI RCCGGY 1 cut(s) 484
BssECI CCNNGG 2 cut(s) 198, 1564
BssNI GRCGYC 2 cut(s) 258, 270
Bst2UI CCWGG 3 cut(s) 47, 1565, 2154
Bst4CI ACNGT 7 cut(s) 418, 785, 913, 1190, 1246, 1658, 2336
Bst6I CTCTTC 5 cut(s) 538, 600, 843, 1399, 2439
BstACI GRCGYC 2 cut(s) 258, 270
BstAPI GCANNNNNTGC 1 cut(s) 1505
BstAUI TGTACA 1 cut(s) 285
BstBAI YACGTR 1 cut(s) 1495
BstBI TTCGAA 2 cut(s) 115, 245
BstC8I GCNNGC 9 cut(s) 233, 347, 482, 486, 503, 547, 651, 1224, 2303
BstDEI CTNAG 4 cut(s) 153, 811, 2192, 2252
BstDSI CCRYGG 1 cut(s) 198
BstF5I GGATG 5 cut(s) 1121, 1180, 1771, 2002, 2395
BstFNI CGCG 3 cut(s) 101, 200, 210
BstHHI GCGC 2 cut(s) 205, 1857
BstMAI GTCTC 8 cut(s) 163, 535, 814, 868, 878, 958, 1565, 2266
BstMCI CGRYCG 2 cut(s) 36, 40
BstMWI GCNNNNNNNGC 8 cut(s) 194, 481, 502, 650, 954, 1505, 1753, 1854
BstNI CCWGG 3 cut(s) 47, 1565, 2154
BstNSI RCATGY 2 cut(s) 854, 1226
BstSCI CCNGG 6 cut(s) 45, 54, 393, 1563, 1590, 2152
BstSFI CTRYAG 3 cut(s) 909, 946, 2267
BstUI CGCG 3 cut(s) 101, 200, 210
BstV1I GCAGC 7 cut(s) 2, 616, 640, 804, 1511, 2058, 2317
BstV2I GAAGAC 3 cut(s) 345, 1092, 1358
BstX2I RGATCY 3 cut(s) 661, 1417, 2023
BstYI RGATCY 3 cut(s) 661, 1417, 2023
Bsu15I ATCGAT 2 cut(s) 36, 1216
BsuRI GGCC 5 cut(s) 50, 398, 496, 2197, 2412
BsuTUI ATCGAT 2 cut(s) 36, 1216
BtgI CCRYGG 1 cut(s) 198
BtsCI GGATG 5 cut(s) 1121, 1180, 1771, 2002, 2395
BtsI GCAGTG 2 cut(s) 1006, 1547
BtsIMutI CAGTG 4 cut(s) 268, 1006, 1547, 1654
Cac8I GCNNGC 9 cut(s) 233, 347, 482, 486, 503, 547, 651, 1224, 2303
CaiI CAGNNNCTG 1 cut(s) 2066
CciI TCATGA 1 cut(s) 1239
CfoI GCGC 2 cut(s) 205, 1857
Cfr10I RCCGGY 1 cut(s) 484
Cfr13I GGNCC 3 cut(s) 43, 171, 371
Cfr42I CCGCGG 1 cut(s) 201
ClaI ATCGAT 2 cut(s) 36, 1216
CseI GACGC 3 cut(s) 107, 247, 259
Csp6I GTAC 2 cut(s) 286, 2008
CspCI CAANNNNNGTGG 2 cut(s) 1849, 1884
CviAII CATG 9 cut(s) 368, 633, 851, 988, 1223, 1240, 1852, 1881, 1937
CviQI GTAC 2 cut(s) 286, 2008
DdeI CTNAG 4 cut(s) 153, 811, 2192, 2252
DriI GACNNNNNGTC 1 cut(s) 787
EaeI YGGCCR 2 cut(s) 396, 2410
Eam1104I CTCTTC 5 cut(s) 538, 600, 843, 1399, 2439
Eam1105I GACNNNNNGTC 1 cut(s) 787
EarI CTCTTC 5 cut(s) 538, 600, 843, 1399, 2439
EciI GGCGGA 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 395
Eco31I GGTCTC 3 cut(s) 163, 535, 1565
Eco47I GGWCC 3 cut(s) 43, 171, 371
Eco57I CTGAAG 3 cut(s) 687, 824, 1086
Eco72I CACGTG 1 cut(s) 1495
Eco88I CYCGRG 1 cut(s) 2185
EcoRI GAATTC 3 cut(s) 279, 1485, 2394
EcoRII CCWGG 3 cut(s) 45, 1563, 2152
EcoT22I ATGCAT 1 cut(s) 1525
EcoT38I GRGCYC 1 cut(s) 395
FaeI CATG 9 cut(s) 371, 636, 854, 991, 1226, 1243, 1855, 1884, 1940
FalI AAGNNNNNCTT 2 cut(s) 1141, 1173
FaqI GGGAC 2 cut(s) 56, 184
FatI CATG 9 cut(s) 367, 632, 850, 987, 1222, 1239, 1851, 1880, 1936
FauI CCCGC 2 cut(s) 15, 160
FokI GGATG 5 cut(s) 1108, 1187, 1778, 2009, 2402
FriOI GRGCYC 1 cut(s) 395
FspAI RTGCGCAY 1 cut(s) 1856
FspBI CTAG 4 cut(s) 615, 1820, 2018, 2370
FspI TGCGCA 1 cut(s) 1856
GlaI GCGC 2 cut(s) 204, 1856
GsuI CTGGAG 3 cut(s) 1377, 1442, 1983
HaeIII GGCC 5 cut(s) 50, 398, 496, 2197, 2412
HapII CCGG 9 cut(s) 26, 56, 75, 276, 374, 395, 485, 1129, 1592
HgaI GACGC 3 cut(s) 107, 247, 259
HhaI GCGC 2 cut(s) 205, 1857
Hin1I GRCGYC 2 cut(s) 258, 270
Hin1II CATG 9 cut(s) 371, 636, 854, 991, 1226, 1243, 1855, 1884, 1940
Hin6I GCGC 2 cut(s) 203, 1855
HinP1I GCGC 2 cut(s) 203, 1855
HpaII CCGG 9 cut(s) 26, 56, 75, 276, 374, 395, 485, 1129, 1592
HphI GGTGA 6 cut(s) 307, 430, 776, 1622, 1636, 2234
Hpy166II GTNNAC 3 cut(s) 298, 848, 919
Hpy8I GTNNAC 3 cut(s) 298, 848, 919
Hpy99I CGWCG 3 cut(s) 101, 166, 275
HpyAV CCTTC 8 cut(s) 241, 445, 520, 711, 826, 1061, 1526, 1561
HpyCH4III ACNGT 7 cut(s) 418, 785, 913, 1190, 1246, 1658, 2336
HpyCH4IV ACGT 3 cut(s) 120, 1494, 2094
HpyF10VI GCNNNNNNNGC 8 cut(s) 194, 481, 502, 650, 954, 1505, 1753, 1854
HpyF3I CTNAG 4 cut(s) 153, 811, 2192, 2252
HpySE526I ACGT 3 cut(s) 120, 1494, 2094
Hsp92I GRCGYC 2 cut(s) 258, 270
Hsp92II CATG 9 cut(s) 371, 636, 854, 991, 1226, 1243, 1855, 1884, 1940
HspAI GCGC 2 cut(s) 203, 1855
KroI GCCGGC 1 cut(s) 484
KroNI GCCGGC 1 cut(s) 486
KspI CCGCGG 1 cut(s) 201
LguI GCTCTTC 1 cut(s) 843
LmnI GCTCC 3 cut(s) 28, 931, 1785
Lsp1109I GCAGC 7 cut(s) 2, 616, 640, 804, 1511, 2058, 2317
LweI GCATC 6 cut(s) 1006, 1096, 1130, 1132, 1532, 1866
MaeI CTAG 4 cut(s) 615, 1820, 2018, 2370
MaeII ACGT 3 cut(s) 120, 1494, 2094
MaeIII GTNAC 8 cut(s) 58, 77, 412, 732, 1628, 1652, 1792, 2095
MbiI CCGCTC 1 cut(s) 152
MfeI CAATTG 2 cut(s) 878, 2383
MflI RGATCY 3 cut(s) 661, 1417, 2023
MhlI GDGCHC 1 cut(s) 395
MlsI TGGCCA 1 cut(s) 2412
MluNI TGGCCA 1 cut(s) 2412
MlyI GAGTC 7 cut(s) 87, 425, 797, 947, 1636, 1682, 2177
MmeI TCCRAC 3 cut(s) 1716, 2051, 2201
Mox20I TGGCCA 1 cut(s) 2412
Mph1103I ATGCAT 1 cut(s) 1525
MroNI GCCGGC 1 cut(s) 484
MroXI GAANNNNTTC 3 cut(s) 251, 1009, 1156
MscI TGGCCA 1 cut(s) 2412
MseI TTAA 8 cut(s) 740, 1112, 1148, 1248, 1380, 1698, 1749, 2147
MslI CAYNNNNRTG 2 cut(s) 1453, 1780
Msp20I TGGCCA 1 cut(s) 2412
MspA1I CMGCKG 2 cut(s) 10, 200
MspI CCGG 9 cut(s) 26, 56, 75, 276, 374, 395, 485, 1129, 1592
MspR9I CCNGG 6 cut(s) 47, 56, 395, 1565, 1592, 2154
MunI CAATTG 2 cut(s) 878, 2383
MvaI CCWGG 3 cut(s) 47, 1565, 2154
MvnI CGCG 3 cut(s) 101, 200, 210
MwoI GCNNNNNNNGC 8 cut(s) 194, 481, 502, 650, 954, 1505, 1753, 1854
NaeI GCCGGC 1 cut(s) 486
NciI CCSGG 3 cut(s) 56, 395, 1592
NgoMIV GCCGGC 1 cut(s) 484
NlaIII CATG 9 cut(s) 371, 636, 854, 991, 1226, 1243, 1855, 1884, 1940
NlaIV GGNNCC 4 cut(s) 44, 108, 172, 1849
NmeAIII GCCGAG 2 cut(s) 165, 261
NmuCI GTSAC 6 cut(s) 58, 77, 412, 1628, 1652, 1792
NsbI TGCGCA 1 cut(s) 1856
NsiI ATGCAT 1 cut(s) 1525
NspI RCATGY 2 cut(s) 854, 1226
NspV TTCGAA 2 cut(s) 115, 245
PaeI GCATGC 1 cut(s) 1226
PaeR7I CTCGAG 1 cut(s) 2185
PagI TCATGA 1 cut(s) 1239
PciI ACATGT 1 cut(s) 850
PciSI GCTCTTC 1 cut(s) 843
PdiI GCCGGC 1 cut(s) 486
PdmI GAANNNNTTC 3 cut(s) 251, 1009, 1156
PfeI GAWTC 5 cut(s) 176, 242, 382, 1010, 1199
PflFI GACNNNGTC 1 cut(s) 56
Ple19I CGATCG 2 cut(s) 36, 40
PleI GAGTC 7 cut(s) 87, 425, 796, 946, 1635, 1682, 2177
PmaCI CACGTG 1 cut(s) 1495
PmlI CACGTG 1 cut(s) 1495
PpsI GAGTC 7 cut(s) 87, 425, 796, 946, 1635, 1682, 2177
Ppu21I YACGTR 1 cut(s) 1495
PscI ACATGT 1 cut(s) 850
PsiI TTATAA 1 cut(s) 2343
Psp6I CCWGG 3 cut(s) 45, 1563, 2152
PspCI CACGTG 1 cut(s) 1495
PspGI CCWGG 3 cut(s) 45, 1563, 2152
PspN4I GGNNCC 4 cut(s) 44, 108, 172, 1849
PspPI GGNCC 3 cut(s) 43, 171, 371
PspXI VCTCGAGB 1 cut(s) 2185
PstI CTGCAG 1 cut(s) 950
PstNI CAGNNNCTG 1 cut(s) 2066
PsuI RGATCY 3 cut(s) 661, 1417, 2023
PsyI GACNNNGTC 1 cut(s) 56
PvuI CGATCG 2 cut(s) 36, 40
RsaI GTAC 2 cut(s) 287, 2009
RsaNI GTAC 2 cut(s) 286, 2008
RseI CAYNNNNRTG 2 cut(s) 1453, 1780
SacII CCGCGG 1 cut(s) 201
SapI GCTCTTC 1 cut(s) 843
SaqAI TTAA 8 cut(s) 740, 1112, 1148, 1248, 1380, 1698, 1749, 2147
Sau96I GGNCC 3 cut(s) 43, 171, 371
SchI GAGTC 7 cut(s) 87, 425, 797, 947, 1636, 1682, 2177
ScrFI CCNGG 6 cut(s) 47, 56, 395, 1565, 1592, 2154
SduI GDGCHC 1 cut(s) 395
SfaNI GCATC 6 cut(s) 1006, 1096, 1130, 1132, 1532, 1866
SfcI CTRYAG 3 cut(s) 909, 946, 2267
Sfr274I CTCGAG 1 cut(s) 2185
Sfr303I CCGCGG 1 cut(s) 201
SfuI TTCGAA 2 cut(s) 115, 245
SgrBI CCGCGG 1 cut(s) 201
SinI GGWCC 3 cut(s) 43, 171, 371
SlaI CTCGAG 1 cut(s) 2185
SmiMI CAYNNNNRTG 2 cut(s) 1453, 1780
SmlI CTYRAG 2 cut(s) 2185, 2431
SmoI CTYRAG 2 cut(s) 2185, 2431
SphI GCATGC 1 cut(s) 1226
SsiI CCGC 8 cut(s) 8, 101, 104, 150, 167, 195, 198, 200
SspI AATATT 1 cut(s) 1339
SspMI CTAG 4 cut(s) 615, 1820, 2018, 2370
StyD4I CCNGG 6 cut(s) 45, 54, 393, 1563, 1590, 2152
TaaI ACNGT 7 cut(s) 418, 785, 913, 1190, 1246, 1658, 2336
TaiI ACGT 3 cut(s) 123, 1497, 2097
TaqII GACCGA 2 cut(s) 188, 1932
TatI WGTACW 2 cut(s) 285, 2007
TauI GCSGC 3 cut(s) 104, 200, 203
TfiI GAWTC 5 cut(s) 176, 242, 382, 1010, 1199
Tru1I TTAA 8 cut(s) 740, 1112, 1148, 1248, 1380, 1698, 1749, 2147
Tru9I TTAA 8 cut(s) 740, 1112, 1148, 1248, 1380, 1698, 1749, 2147
TscAI CASTG 4 cut(s) 268, 1006, 1547, 1661
TseFI GTSAC 6 cut(s) 58, 77, 412, 1628, 1652, 1792
TseI GCWGC 7 cut(s) 15, 629, 653, 817, 1499, 2071, 2305
Tsp45I GTSAC 6 cut(s) 58, 77, 412, 1628, 1652, 1792
TspGWI ACGGA 2 cut(s) 973, 1053
TspRI CASTG 4 cut(s) 268, 1006, 1547, 1661
Tth111I GACNNNGTC 1 cut(s) 56
VpaK11BI GGWCC 3 cut(s) 43, 171, 371
XapI RAATTY 6 cut(s) 279, 1485, 1676, 1827, 1892, 2394
XbaI TCTAGA 1 cut(s) 614
XceI RCATGY 2 cut(s) 854, 1226
XhoI CTCGAG 1 cut(s) 2185
XmnI GAANNNNTTC 3 cut(s) 251, 1009, 1156
XspI CTAG 4 cut(s) 615, 1820, 2018, 2370
Zsp2I ATGCAT 1 cut(s) 1525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.