RchiOBHm_Chr4g0440851

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005085
Physical Location & Seq
Reverse (-)
62815078 .. 62819832
4755 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40879

Sequence Viewer

Length: 1692 bp
ATGAAGATTGACACTTCGTTTTGCTGCTTCATTTTGTTTCTGGTTTTGGGTTCTATCAGCTGCGTGTCAGCTCTAGCGAATGATGGAGTTGTAGCCAACAGAAGATCTCTCAAATTTAGTCTAGGAGATGAGAATCTGGGGTCTTGGCAAAATGAGCTATCTGAGACTGCAGAGGCCCCAGGGCCTTCGAGCGAAGGTCCTGAGCTTGTATTGGCGGCGAAAAGGACCAAGAGGCCTGATATTCTTGATGGGTTCAAGCGTTATCGAGGAGGATGGGACATAGCGAATCGCCATTACTGGGCTTCTGTTGGATTCACAGGTGCCGCTGGTTTCATTATTTCTGTCCTATGGTTTGTTGCTTTTGGCATCGTTCTTGTGGTTCATCATTGCTGTGGATGGAGAATAAACATTAAAGATGAAGGATCACCCCGCTCACAGAAGATGTGTCTGATTATTCTGATAGTCTTCACATGTGCTGCCACGGTTGGATGCATTCTTCTTTCTGTTGGGCAAGCTGAGTTTCATGAAGAAGTCCTGCATACTCTAAATTTCGTTGTAAACCAGTCAGACTACACTGTACAGACCTTGAGAAACGTCACAGAGTATCTGTCCCTTGCAAAGGATATTAATGTGGCCCAGGTTTTCCTACCTACTGATGTCATGGATAGTATTGACAAGTTGAATGTGGATCTCAACACTGCAGCAGATACATTAACAGAGAAGACAAGTGAAAATTCTGACAAAGTCAAAAAAGTCTTCAGTATTGTGCGTTCCGTTTTAATTGCCGTAGCAGCAGTAATGCTTTTCCTAGCTGTGATCGGTCTTCTTCTGTCCGTCCTTCGGCACCAACATGCAATTCACATATTCATTGTTAGTGGATGGCTACTTGTTGCAATTACATTCATTCTTTGTGGAGCCTTTGTGATCCTCAACAATGCTGTTTCTGACACCTGTATGGCAATGGAAGAATGGGTAGAAAATCCCCACGCTGAAACAGCACTTAGCAACGTCCTTCCATGTGTTGACCAGAAAACCACAAACCAGACACTAAGCCAGAGTAAAAGAATTATCAATGACATGGTCACTGTTGTCAACACGTTCATCTACACCTATGCCAATACGTATCCATCGCATTCTGATCCGTATTATTACAATCAGTCTGGACCTATGATGCCAGCTCTTTGTTACCCTTATGATTCTGAGTTAAGAGATACTCAGTGTGGGGATCAACAGGTGTCTATTACAAATGCTTCTTTGGTTTGGCAGAACTATACATGTGAAGTCTCAGCATCTGGGATGTGCATCACTCCCGGAAGGGTGAAGCCAGAGATCTACACTCAGCTAGTTGCAGCAGTTAATGAGAGCTATGCACTACAGCACTATACCCCTCCTTTACTCAGCCTCCAAGATTGTAATTTTGTCCGAGACACATTTCGAACAATCACCTCGAGTTATTGCCCCCCATTGGACCACTACCTGAAAATTGTGAATGCAGGATTGGCCCTAATATCAGTTGGAGTCTTGCTCTGTCTTCTTCTCTGGGTACTCTATGCAAACCGCCCCCAAAGGGAGGAAGCGTTTGTGAAACAATCCTTACCCGTAAAAGACAGGAGTATGAAGAGCAGTTCAAACAATCATAGTCATAATAGCAGCAGCGATGTAACAACATCCAACATATCAAACGGAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

563

Amino Acids

62.19

Weight (kDa)

5.78

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tweety PF04906 96 - 377 2.1e-06 Tweety
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 320, 843
AccBSI CCGCTC 1 cut(s) 432
AciI CCGC 4 cut(s) 215, 324, 430, 1558
AclWI GGATC 5 cut(s) 430, 696, 919, 1133, 1233
AcsI RAATTY 3 cut(s) 113, 547, 733
AcuI CTGAAG 1 cut(s) 742
AfaI GTAC 2 cut(s) 579, 1545
AfiI CCNNNNNNNGG 6 cut(s) 298, 619, 840, 1465, 1567, 1570
AflIII ACRYGT 3 cut(s) 470, 1095, 1274
AgsI TTSAA 3 cut(s) 256, 682, 1629
AjnI CCWGG 2 cut(s) 178, 636
AjuI GAANNNNNNNTTGG 4 cut(s) 840, 872, 1481, 1513
AluBI AGCT 9 cut(s) 60, 71, 157, 205, 515, 812, 1178, 1342, 1365
AluI AGCT 9 cut(s) 60, 71, 157, 205, 515, 812, 1178, 1342, 1365
Alw26I GTCTC 3 cut(s) 158, 1288, 1419
AlwI GGATC 5 cut(s) 430, 696, 919, 1133, 1233
AlwNI CAGNNNCTG 1 cut(s) 1292
Ama87I CYCGRG 1 cut(s) 1447
AoxI GGCC 5 cut(s) 174, 182, 233, 633, 1500
ApeKI GCWGC 8 cut(s) 24, 60, 476, 701, 791, 1349, 1650, 1653
ApoI RAATTY 3 cut(s) 113, 547, 733
AseI ATTAAT 1 cut(s) 627
AspS9I GGNCC 8 cut(s) 175, 182, 197, 225, 634, 1163, 1468, 1501
AsuC2I CCSGG 1 cut(s) 1311
AsuHPI GGTGA 3 cut(s) 417, 1330, 1435
AsuII TTCGAA 1 cut(s) 1435
AvaI CYCGRG 1 cut(s) 1447
AvaII GGWCC 4 cut(s) 197, 225, 1163, 1468
BanI GGYRCC 2 cut(s) 320, 843
BbsI GAAGAC 5 cut(s) 457, 728, 748, 815, 1523
BbvI GCAGC 8 cut(s) 11, 47, 463, 713, 803, 1361, 1662, 1665
BccI CCATC 6 cut(s) 77, 242, 267, 390, 873, 1135
BceAI ACGGC 1 cut(s) 770
BciT130I CCWGG 2 cut(s) 180, 638
BciVI GTATCC 1 cut(s) 1134
BcnI CCSGG 1 cut(s) 1311
BcoDI GTCTC 3 cut(s) 158, 1288, 1419
BfaI CTAG 4 cut(s) 74, 122, 809, 1343
BfmI CTRYAG 3 cut(s) 168, 699, 1373
BfuI GTATCC 1 cut(s) 1134
BglII AGATCT 2 cut(s) 104, 1329
Bme1390I CCNGG 3 cut(s) 180, 638, 1311
Bme18I GGWCC 4 cut(s) 197, 225, 1163, 1468
BmeT110I CYCGRG 1 cut(s) 1447
BmgT120I GGNCC 8 cut(s) 175, 182, 197, 225, 634, 1163, 1468, 1501
BmiI GGNNCC 4 cut(s) 177, 322, 845, 916
BmrFI CCNGG 3 cut(s) 180, 638, 1311
BmrI ACTGGG 1 cut(s) 307
BmsI GCATC 5 cut(s) 375, 479, 1161, 1298, 1311
BmuI ACTGGG 1 cut(s) 307
BpiI GAAGAC 5 cut(s) 457, 728, 748, 815, 1523
BplI GAGNNNNNCTC 2 cut(s) 1509, 1541
Bpu10I CCTNAGC 1 cut(s) 201
Bpu14I TTCGAA 1 cut(s) 1435
BpuEI CTTGAG 1 cut(s) 607
BpuMI CCSGG 1 cut(s) 1311
BsaAI YACGTR 1 cut(s) 1122
BsaBI GATNNNNATC 2 cut(s) 132, 1301
BsaJI CCNNGG 4 cut(s) 178, 179, 480, 636
BsaXI ACNNNNNCTCC 6 cut(s) 906, 936, 1386, 1416, 1562, 1592
Bsc4I CCNNNNNNNGG 6 cut(s) 298, 619, 840, 1465, 1567, 1570
Bse1I ACTGG 2 cut(s) 302, 562
Bse3DI GCAATG 2 cut(s) 385, 966
Bse8I GATNNNNATC 2 cut(s) 132, 1301
BseBI CCWGG 2 cut(s) 180, 638
BseDI CCNNGG 4 cut(s) 178, 179, 480, 636
BseGI GGATG 6 cut(s) 278, 401, 494, 884, 1302, 1667
BseJI GATNNNNATC 2 cut(s) 132, 1301
BseLI CCNNNNNNNGG 6 cut(s) 298, 619, 840, 1465, 1567, 1570
BseMI GCAATG 2 cut(s) 385, 966
BseMII CTCAG 8 cut(s) 153, 192, 507, 1191, 1229, 1299, 1352, 1411
BseNI ACTGG 2 cut(s) 302, 562
BseRI GAGGAG 1 cut(s) 282
BseXI GCAGC 8 cut(s) 11, 47, 463, 713, 803, 1361, 1662, 1665
BshFI GGCC 5 cut(s) 176, 184, 235, 635, 1502
BshNI GGYRCC 2 cut(s) 320, 843
BsiHKCI CYCGRG 1 cut(s) 1447
BsiSI CCGG 1 cut(s) 1311
BslFI GGGAC 2 cut(s) 290, 595
BslI CCNNNNNNNGG 6 cut(s) 298, 619, 840, 1465, 1567, 1570
BsmAI GTCTC 3 cut(s) 158, 1288, 1419
BsmFI GGGAC 2 cut(s) 290, 595
BsmI GAATGC 3 cut(s) 492, 1132, 1495
BsnI GGCC 5 cut(s) 176, 184, 235, 635, 1502
BsoBI CYCGRG 1 cut(s) 1447
Bsp119I TTCGAA 1 cut(s) 1435
Bsp1407I TGTACA 1 cut(s) 577
Bsp143I GATC 8 cut(s) 104, 422, 688, 816, 924, 1138, 1225, 1329
BspACI CCGC 4 cut(s) 215, 324, 430, 1558
BspANI GGCC 5 cut(s) 176, 184, 235, 635, 1502
BspCNI CTCAG 8 cut(s) 154, 193, 508, 1192, 1228, 1298, 1351, 1410
BspHI TCATGA 1 cut(s) 523
BspLI GGNNCC 4 cut(s) 177, 322, 845, 916
BspMAI CTGCAG 2 cut(s) 172, 703
BspPI GGATC 5 cut(s) 430, 696, 919, 1133, 1233
BspQI GCTCTTC 1 cut(s) 1613
BspT104I TTCGAA 1 cut(s) 1435
BspT107I GGYRCC 2 cut(s) 320, 843
BsrBI CCGCTC 1 cut(s) 432
BsrDI GCAATG 2 cut(s) 385, 966
BsrGI TGTACA 1 cut(s) 577
BsrI ACTGG 2 cut(s) 302, 562
BssECI CCNNGG 4 cut(s) 178, 179, 480, 636
BssMI GATC 8 cut(s) 104, 422, 688, 816, 924, 1138, 1225, 1329
Bst2UI CCWGG 2 cut(s) 180, 638
Bst4CI ACNGT 3 cut(s) 484, 577, 1087
Bst6I CTCTTC 1 cut(s) 1613
BstAUI TGTACA 1 cut(s) 577
BstBAI YACGTR 1 cut(s) 1122
BstBI TTCGAA 1 cut(s) 1435
BstC8I GCNNGC 2 cut(s) 513, 1176
BstDSI CCRYGG 1 cut(s) 480
BstENI CCTNNNNNAGG 1 cut(s) 617
BstF5I GGATG 6 cut(s) 278, 401, 494, 884, 1302, 1667
BstKTI GATC 8 cut(s) 107, 425, 691, 819, 927, 1141, 1228, 1332
BstMAI GTCTC 3 cut(s) 158, 1288, 1419
BstMBI GATC 8 cut(s) 104, 422, 688, 816, 924, 1138, 1225, 1329
BstMWI GCNNNNNNNGC 4 cut(s) 154, 791, 995, 1499
BstNI CCWGG 2 cut(s) 180, 638
BstNSI RCATGY 3 cut(s) 474, 854, 1278
BstSCI CCNGG 3 cut(s) 178, 636, 1309
BstSFI CTRYAG 3 cut(s) 168, 699, 1373
BstSNI TACGTA 1 cut(s) 1122
BstV1I GCAGC 8 cut(s) 11, 47, 463, 713, 803, 1361, 1662, 1665
BstV2I GAAGAC 5 cut(s) 457, 728, 748, 815, 1523
BstX2I RGATCY 3 cut(s) 104, 688, 1329
BstYI RGATCY 3 cut(s) 104, 688, 1329
BsuI GTATCC 1 cut(s) 1134
BsuRI GGCC 5 cut(s) 176, 184, 235, 635, 1502
BtgI CCRYGG 1 cut(s) 480
BtgZI GCGATG 2 cut(s) 1113, 1671
BtsCI GGATG 6 cut(s) 278, 401, 494, 884, 1302, 1667
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 4 cut(s) 573, 696, 1083, 1223
Cac8I GCNNGC 2 cut(s) 513, 1176
CaiI CAGNNNCTG 1 cut(s) 1292
CciI TCATGA 1 cut(s) 523
Cfr13I GGNCC 8 cut(s) 175, 182, 197, 225, 634, 1163, 1468, 1501
Csp6I GTAC 2 cut(s) 578, 1544
CviAII CATG 7 cut(s) 471, 524, 661, 851, 1017, 1078, 1275
CviQI GTAC 2 cut(s) 578, 1544
DpnI GATC 8 cut(s) 106, 424, 690, 818, 926, 1140, 1227, 1331
DpnII GATC 8 cut(s) 104, 422, 688, 816, 924, 1138, 1225, 1329
Eam1104I CTCTTC 1 cut(s) 1613
EarI CTCTTC 1 cut(s) 1613
Eco105I TACGTA 1 cut(s) 1122
Eco147I AGGCCT 1 cut(s) 235
Eco47I GGWCC 4 cut(s) 197, 225, 1163, 1468
Eco57I CTGAAG 1 cut(s) 742
Eco88I CYCGRG 1 cut(s) 1447
EcoNI CCTNNNNNAGG 1 cut(s) 617
EcoO109I RGGNCCY 3 cut(s) 175, 182, 197
EcoRII CCWGG 2 cut(s) 178, 636
EcoT22I ATGCAT 1 cut(s) 494
FaeI CATG 7 cut(s) 474, 527, 664, 854, 1020, 1081, 1278
FaqI GGGAC 2 cut(s) 290, 595
FatI CATG 7 cut(s) 470, 523, 660, 850, 1016, 1077, 1274
FauI CCCGC 1 cut(s) 437
FokI GGATG 6 cut(s) 285, 408, 501, 891, 1309, 1654
FspBI CTAG 4 cut(s) 74, 122, 809, 1343
HaeIII GGCC 5 cut(s) 176, 184, 235, 635, 1502
HapII CCGG 1 cut(s) 1311
Hin1II CATG 7 cut(s) 474, 527, 664, 854, 1020, 1081, 1278
HincII GTYRAC 2 cut(s) 1024, 1093
HindII GTYRAC 2 cut(s) 1024, 1093
HinfI GANTC 6 cut(s) 133, 286, 312, 1196, 1518, 1686
HpaII CCGG 1 cut(s) 1311
HphI GGTGA 3 cut(s) 417, 1330, 1435
Hpy166II GTNNAC 3 cut(s) 559, 1024, 1093
Hpy188I TCNGA 9 cut(s) 163, 450, 459, 568, 739, 946, 1138, 1201, 1424
Hpy188III TCNNGA 4 cut(s) 200, 245, 524, 1161
Hpy8I GTNNAC 3 cut(s) 559, 1024, 1093
HpyAV CCTTC 6 cut(s) 188, 195, 413, 848, 1022, 1308
HpyCH4III ACNGT 3 cut(s) 484, 577, 1087
HpyCH4IV ACGT 4 cut(s) 594, 1008, 1097, 1121
HpyF10VI GCNNNNNNNGC 4 cut(s) 154, 791, 995, 1499
HpySE526I ACGT 4 cut(s) 594, 1008, 1097, 1121
Hsp92II CATG 7 cut(s) 474, 527, 664, 854, 1020, 1081, 1278
Kzo9I GATC 8 cut(s) 104, 422, 688, 816, 924, 1138, 1225, 1329
LguI GCTCTTC 1 cut(s) 1613
LmnI GCTCC 1 cut(s) 914
Lsp1109I GCAGC 8 cut(s) 11, 47, 463, 713, 803, 1361, 1662, 1665
LweI GCATC 5 cut(s) 375, 479, 1161, 1298, 1311
MaeI CTAG 4 cut(s) 74, 122, 809, 1343
MaeII ACGT 4 cut(s) 594, 1008, 1097, 1121
MaeIII GTNAC 4 cut(s) 595, 1081, 1184, 1660
MalI GATC 8 cut(s) 106, 424, 690, 818, 926, 1140, 1227, 1331
MbiI CCGCTC 1 cut(s) 432
MboI GATC 8 cut(s) 104, 422, 688, 816, 924, 1138, 1225, 1329
MflI RGATCY 3 cut(s) 104, 688, 1329
MluCI AATT 9 cut(s) 113, 547, 733, 780, 855, 894, 1065, 1414, 1482
MlyI GAGTC 1 cut(s) 1527
MmeI TCCRAC 3 cut(s) 289, 466, 1495
MnlI CCTC 9 cut(s) 166, 225, 260, 263, 938, 1398, 1412, 1456, 1564
Mph1103I ATGCAT 1 cut(s) 494
MseI TTAA 6 cut(s) 411, 627, 713, 779, 1205, 1356
MslI CAYNNNNRTG 3 cut(s) 390, 849, 953
MspA1I CMGCKG 2 cut(s) 60, 326
MspI CCGG 1 cut(s) 1311
MspR9I CCNGG 3 cut(s) 180, 638, 1311
Mva1269I GAATGC 3 cut(s) 492, 1132, 1495
MvaI CCWGG 2 cut(s) 180, 638
MwoI GCNNNNNNNGC 4 cut(s) 154, 791, 995, 1499
NciI CCSGG 1 cut(s) 1311
NdeII GATC 8 cut(s) 104, 422, 688, 816, 924, 1138, 1225, 1329
NlaIII CATG 7 cut(s) 474, 527, 664, 854, 1020, 1081, 1278
NlaIV GGNNCC 4 cut(s) 177, 322, 845, 916
NmuCI GTSAC 2 cut(s) 595, 1081
NsiI ATGCAT 1 cut(s) 494
NspI RCATGY 3 cut(s) 474, 854, 1278
NspV TTCGAA 1 cut(s) 1435
PaeR7I CTCGAG 1 cut(s) 1447
PagI TCATGA 1 cut(s) 523
PasI CCCWGGG 1 cut(s) 179
PceI AGGCCT 1 cut(s) 235
PciI ACATGT 2 cut(s) 470, 1274
PciSI GCTCTTC 1 cut(s) 1613
PctI GAATGC 3 cut(s) 492, 1132, 1495
PfeI GAWTC 4 cut(s) 133, 286, 312, 1196
PflFI GACNNNGTC 2 cut(s) 743, 1079
PfoI TCCNGGA 1 cut(s) 1309
PleI GAGTC 1 cut(s) 1526
PpsI GAGTC 1 cut(s) 1526
Ppu21I YACGTR 1 cut(s) 1122
PpuMI RGGWCCY 1 cut(s) 197
PscI ACATGT 2 cut(s) 470, 1274
PshBI ATTAAT 1 cut(s) 627
Psp5II RGGWCCY 1 cut(s) 197
Psp6I CCWGG 2 cut(s) 178, 636
PspGI CCWGG 2 cut(s) 178, 636
PspN4I GGNNCC 4 cut(s) 177, 322, 845, 916
PspPI GGNCC 8 cut(s) 175, 182, 197, 225, 634, 1163, 1468, 1501
PspPPI RGGWCCY 1 cut(s) 197
PspXI VCTCGAGB 1 cut(s) 1447
PsrI GAACNNNNNNTAC 2 cut(s) 754, 786
PstI CTGCAG 2 cut(s) 172, 703
PstNI CAGNNNCTG 1 cut(s) 1292
PsuI RGATCY 3 cut(s) 104, 688, 1329
PsyI GACNNNGTC 2 cut(s) 743, 1079
PvuII CAGCTG 1 cut(s) 60
RsaI GTAC 2 cut(s) 579, 1545
RsaNI GTAC 2 cut(s) 578, 1544
RseI CAYNNNNRTG 3 cut(s) 390, 849, 953
SapI GCTCTTC 1 cut(s) 1613
SaqAI TTAA 6 cut(s) 411, 627, 713, 779, 1205, 1356
Sau3AI GATC 8 cut(s) 104, 422, 688, 816, 924, 1138, 1225, 1329
Sau96I GGNCC 8 cut(s) 175, 182, 197, 225, 634, 1163, 1468, 1501
SchI GAGTC 1 cut(s) 1527
ScrFI CCNGG 3 cut(s) 180, 638, 1311
SfaNI GCATC 5 cut(s) 375, 479, 1161, 1298, 1311
SfcI CTRYAG 3 cut(s) 168, 699, 1373
Sfr274I CTCGAG 1 cut(s) 1447
SfuI TTCGAA 1 cut(s) 1435
SinI GGWCC 4 cut(s) 197, 225, 1163, 1468
SlaI CTCGAG 1 cut(s) 1447
SmiMI CAYNNNNRTG 3 cut(s) 390, 849, 953
SmlI CTYRAG 2 cut(s) 586, 1447
SmoI CTYRAG 2 cut(s) 586, 1447
SnaBI TACGTA 1 cut(s) 1122
Sse9I AATT 9 cut(s) 113, 547, 733, 780, 855, 894, 1065, 1414, 1482
SseBI AGGCCT 1 cut(s) 235
SsiI CCGC 4 cut(s) 215, 324, 430, 1558
SspMI CTAG 4 cut(s) 74, 122, 809, 1343
StuI AGGCCT 1 cut(s) 235
StyD4I CCNGG 3 cut(s) 178, 636, 1309
TaaI ACNGT 3 cut(s) 484, 577, 1087
TaiI ACGT 4 cut(s) 597, 1011, 1100, 1124
TaqI TCGA 4 cut(s) 188, 265, 1435, 1448
TaqII GACCGA 1 cut(s) 809
TasI AATT 9 cut(s) 113, 547, 733, 780, 855, 894, 1065, 1414, 1482
TatI WGTACW 1 cut(s) 577
TauI GCSGC 2 cut(s) 218, 326
TfiI GAWTC 4 cut(s) 133, 286, 312, 1196
Tru1I TTAA 6 cut(s) 411, 627, 713, 779, 1205, 1356
Tru9I TTAA 6 cut(s) 411, 627, 713, 779, 1205, 1356
TscAI CASTG 4 cut(s) 580, 703, 1090, 1223
TseFI GTSAC 2 cut(s) 595, 1081
TseI GCWGC 8 cut(s) 24, 60, 476, 701, 791, 1349, 1650, 1653
Tsp45I GTSAC 2 cut(s) 595, 1081
TspGWI ACGGA 3 cut(s) 763, 823, 1131
TspRI CASTG 4 cut(s) 580, 703, 1090, 1223
Tth111I GACNNNGTC 2 cut(s) 743, 1079
VpaK11BI GGWCC 4 cut(s) 197, 225, 1163, 1468
VspI ATTAAT 1 cut(s) 627
XagI CCTNNNNNAGG 1 cut(s) 617
XapI RAATTY 3 cut(s) 113, 547, 733
XceI RCATGY 3 cut(s) 474, 854, 1278
XhoI CTCGAG 1 cut(s) 1447
XspI CTAG 4 cut(s) 74, 122, 809, 1343
Zsp2I ATGCAT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.