RchiOBHm_Chr5g0025161

protein trichome birefringence-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0015726
Physical Location & Seq
Forward (+)
19143843 .. 19146997
3155 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30485

Sequence Viewer

Length: 549 bp
ATGGCCCCTTCATCATCGGAGCATCCGGGAAATTTTTCTGTCAATCGATGGACTACGAAAGCTCGTCTGCAAATTTACCAGATGCCCACCCTTCTCAATGAGATTTGGAACCCCAACCCCAATCTCAAATGCCCACTCTTCTCTTTCAAAGGAGTACTAGAGAAGAAGAACAATGAGTCGCAAATAGGCACCAAATGCTACTTGTTTGATGGGAGTTGGATTTACGATGATACATACCCACTCTACGACACATCCAGCTGCCCTTTTATTGACAGAGAGTTCGACTGTCAGAAAAATGGATGCCCGGAGTTCGACTCTCTGAGCTATAACCAGTGGCAGTCACTAACATGTATGCTGCATTCTGCAGTACCCAAAGCCAAATATACTTCATCCAGTAAAGGAGATTTCTATACGCTTCTTTTGCCAGAATATGAAGTTTCTGTGATGTTTTCTCGCAATAAGTTTCTAGTGGATCTGGTCCAAACAAAGGAGGGCATGGTTCTAAACCTTGACTCTATTGAAGGAGGGCATGGTTCACAGCTTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

182

Amino Acids

20.85

Weight (kDa)

5.35

Isoelectric Point (pI)

48.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 65 - 117 1.7e-14 PMR5 N terminal Domain
PC-Esterase PF13839 105 - 176 2.8e-14 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 188
AclWI GGATC 1 cut(s) 480
AcsI RAATTY 2 cut(s) 31, 72
AfaI GTAC 2 cut(s) 156, 369
AfiI CCNNNNNNNGG 1 cut(s) 487
AflIII ACRYGT 1 cut(s) 347
AgsI TTSAA 2 cut(s) 148, 521
AluBI AGCT 4 cut(s) 62, 258, 324, 541
AluI AGCT 4 cut(s) 62, 258, 324, 541
AlwI GGATC 1 cut(s) 480
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 258, 355
ApoI RAATTY 2 cut(s) 31, 72
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
Asp700I GAANNNNTTC 1 cut(s) 34
AspS9I GGNCC 2 cut(s) 4, 478
AsuC2I CCSGG 2 cut(s) 27, 305
AvaII GGWCC 1 cut(s) 478
BanI GGYRCC 1 cut(s) 188
BbvI GCAGC 2 cut(s) 245, 342
BccI CCATC 2 cut(s) 42, 203
BcnI CCSGG 2 cut(s) 27, 305
BfaI CTAG 2 cut(s) 158, 467
BfmI CTRYAG 1 cut(s) 363
BisI GCNGC 2 cut(s) 259, 356
BlsI GCNGC 2 cut(s) 260, 357
BmcAI AGTACT 1 cut(s) 156
Bme1390I CCNGG 2 cut(s) 27, 305
Bme18I GGWCC 1 cut(s) 478
BmgT120I GGNCC 2 cut(s) 4, 478
BmiI GGNNCC 3 cut(s) 6, 110, 190
BmrFI CCNGG 2 cut(s) 27, 305
BmsI GCATC 3 cut(s) 31, 72, 290
BplI GAGNNNNNCTC 2 cut(s) 299, 331
BpuMI CCSGG 2 cut(s) 27, 305
Bsa29I ATCGAT 1 cut(s) 46
Bsc4I CCNNNNNNNGG 1 cut(s) 487
Bse1I ACTGG 2 cut(s) 331, 393
BseCI ATCGAT 1 cut(s) 46
BseGI GGATG 4 cut(s) 22, 251, 305, 389
BseLI CCNNNNNNNGG 1 cut(s) 487
BseMII CTCAG 1 cut(s) 311
BseNI ACTGG 2 cut(s) 331, 393
BseXI GCAGC 2 cut(s) 245, 342
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 188
BshVI ATCGAT 1 cut(s) 46
BsiSI CCGG 2 cut(s) 26, 305
BslI CCNNNNNNNGG 1 cut(s) 487
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 1 cut(s) 472
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 312
BspDI ATCGAT 1 cut(s) 46
BspLI GGNNCC 3 cut(s) 6, 110, 190
BspMAI CTGCAG 1 cut(s) 367
BspPI GGATC 1 cut(s) 480
BspT107I GGYRCC 1 cut(s) 188
BsrI ACTGG 2 cut(s) 331, 393
BssMI GATC 1 cut(s) 472
Bst4CI ACNGT 1 cut(s) 287
Bst6I CTCTTC 1 cut(s) 143
BstAPI GCANNNNNTGC 1 cut(s) 195
BstDEI CTNAG 1 cut(s) 320
BstF5I GGATG 4 cut(s) 22, 251, 305, 389
BstKTI GATC 1 cut(s) 475
BstMBI GATC 1 cut(s) 472
BstMWI GCNNNNNNNGC 2 cut(s) 195, 421
BstNSI RCATGY 1 cut(s) 351
BstSCI CCNGG 2 cut(s) 25, 303
BstSFI CTRYAG 1 cut(s) 363
BstV1I GCAGC 2 cut(s) 245, 342
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
Bsu15I ATCGAT 1 cut(s) 46
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 46
BtsCI GGATG 4 cut(s) 22, 251, 305, 389
BtsIMutI CAGTG 1 cut(s) 338
Cfr13I GGNCC 2 cut(s) 4, 478
ClaI ATCGAT 1 cut(s) 46
Csp6I GTAC 2 cut(s) 155, 368
CviAII CATG 3 cut(s) 348, 496, 530
CviJI RGCY 6 cut(s) 5, 62, 258, 324, 377, 541
CviKI_1 RGCY 6 cut(s) 5, 62, 258, 324, 377, 541
CviQI GTAC 2 cut(s) 155, 368
DdeI CTNAG 1 cut(s) 320
DpnI GATC 1 cut(s) 474
DpnII GATC 1 cut(s) 472
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Eco47I GGWCC 1 cut(s) 478
FaeI CATG 3 cut(s) 351, 499, 533
FaiI YATR 9 cut(s) 235, 327, 349, 353, 384, 411, 432, 497, 531
FatI CATG 3 cut(s) 347, 495, 529
Fnu4HI GCNGC 2 cut(s) 259, 356
FokI GGATG 4 cut(s) 9, 238, 312, 376
Fsp4HI GCNGC 2 cut(s) 259, 356
FspBI CTAG 2 cut(s) 158, 467
GluI GCNGC 2 cut(s) 259, 356
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 26, 305
Hin1II CATG 3 cut(s) 351, 499, 533
HinfI GANTC 3 cut(s) 176, 314, 512
HpaII CCGG 2 cut(s) 26, 305
Hpy166II GTNNAC 1 cut(s) 536
Hpy188I TCNGA 3 cut(s) 19, 291, 321
Hpy8I GTNNAC 1 cut(s) 536
HpyAV CCTTC 3 cut(s) 18, 101, 515
HpyCH4III ACNGT 1 cut(s) 287
HpyCH4V TGCA 3 cut(s) 70, 358, 365
HpyF10VI GCNNNNNNNGC 2 cut(s) 195, 421
HpyF3I CTNAG 1 cut(s) 320
Hsp92II CATG 3 cut(s) 351, 499, 533
Kzo9I GATC 1 cut(s) 472
LmnI GCTCC 1 cut(s) 19
LpnPI CCDG 8 cut(s) 39, 92, 268, 318, 344, 406, 438, 461
Lsp1109I GCAGC 2 cut(s) 245, 342
LweI GCATC 3 cut(s) 31, 72, 290
MaeI CTAG 2 cut(s) 158, 467
MaeIII GTNAC 1 cut(s) 339
MalI GATC 1 cut(s) 474
MboI GATC 1 cut(s) 472
MboII GAAGA 3 cut(s) 130, 175, 178
MflI RGATCY 1 cut(s) 472
MluCI AATT 2 cut(s) 31, 72
MlyI GAGTC 3 cut(s) 185, 308, 506
MmeI TCCRAC 1 cut(s) 197
MnlI CCTC 2 cut(s) 484, 518
MroXI GAANNNNTTC 1 cut(s) 34
MslI CAYNNNNRTG 1 cut(s) 346
MspA1I CMGCKG 1 cut(s) 258
MspI CCGG 2 cut(s) 26, 305
MspR9I CCNGG 2 cut(s) 27, 305
Mva1269I GAATGC 1 cut(s) 358
MwoI GCNNNNNNNGC 2 cut(s) 195, 421
NciI CCSGG 2 cut(s) 27, 305
NdeII GATC 1 cut(s) 472
NlaIII CATG 3 cut(s) 351, 499, 533
NlaIV GGNNCC 3 cut(s) 6, 110, 190
NmuCI GTSAC 1 cut(s) 339
NspI RCATGY 1 cut(s) 351
PciI ACATGT 1 cut(s) 347
PctI GAATGC 1 cut(s) 358
PdmI GAANNNNTTC 1 cut(s) 34
PfoI TCCNGGA 1 cut(s) 25
PkrI GCNGC 2 cut(s) 260, 357
PleI GAGTC 3 cut(s) 184, 308, 506
PpsI GAGTC 3 cut(s) 184, 308, 506
PscI ACATGT 1 cut(s) 347
PspN4I GGNNCC 3 cut(s) 6, 110, 190
PspPI GGNCC 2 cut(s) 4, 478
PstI CTGCAG 1 cut(s) 367
PsuI RGATCY 1 cut(s) 472
PvuII CAGCTG 1 cut(s) 258
RsaI GTAC 2 cut(s) 156, 369
RsaNI GTAC 2 cut(s) 155, 368
RseI CAYNNNNRTG 1 cut(s) 346
SatI GCNGC 2 cut(s) 259, 356
Sau3AI GATC 1 cut(s) 472
Sau96I GGNCC 2 cut(s) 4, 478
ScaI AGTACT 1 cut(s) 156
SchI GAGTC 3 cut(s) 185, 308, 506
ScrFI CCNGG 2 cut(s) 27, 305
SetI ASST 5 cut(s) 64, 260, 326, 510, 543
SfaNI GCATC 3 cut(s) 31, 72, 290
SfcI CTRYAG 1 cut(s) 363
SinI GGWCC 1 cut(s) 478
SmiMI CAYNNNNRTG 1 cut(s) 346
Sse9I AATT 2 cut(s) 31, 72
SspMI CTAG 2 cut(s) 158, 467
StyD4I CCNGG 2 cut(s) 25, 303
TaaI ACNGT 1 cut(s) 287
TaqI TCGA 3 cut(s) 46, 282, 312
TasI AATT 2 cut(s) 31, 72
TatI WGTACW 1 cut(s) 154
TscAI CASTG 1 cut(s) 338
TseFI GTSAC 1 cut(s) 339
TseI GCWGC 2 cut(s) 258, 355
Tsp45I GTSAC 1 cut(s) 339
TspDTI ATGAA 2 cut(s) 378, 447
TspRI CASTG 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 478
XapI RAATTY 2 cut(s) 31, 72
XceI RCATGY 1 cut(s) 351
XmnI GAANNNNTTC 1 cut(s) 34
XspI CTAG 2 cut(s) 158, 467
ZrmI AGTACT 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.