RchiOBHm_Chr5g0063471

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0016031
Physical Location & Seq
Reverse (-)
69187305 .. 69190524
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33957

Sequence Viewer

Length: 1434 bp
ATGAAGCTCACTCGTCCGCATCGTCCCCTCCCAAACCCTCTCATTCACTCTAAACCCAGAAATCTCAGTACCTCTTCCAATTCCACCCCCAAAGATAATGACGAAAACGACGACCCCCACTTCATTTCCAACCTATCCGACGTCGTTCGAGGGAACCAGAGCTGGAGAATCGCCTTCAGCGACCCCTTCATTTCGAGCTCGTTAAAACCCCACCACGTCGAGAAGGTTCTGATCCAGAACGTAAGGAATCCCAGGAGTTTGTGTCTTTGTCGTGCAAGCATGATGGCTGAAGCAAGTAAATTCTTTGATGAAATGGTGGAAAGGGGCATTATGCCAAACGAGGTGACGTACAATCTTTTGATTGATGGGTACTGTAGGGAAGGTAACATTACTAGAGCTTTTGAATTGCTTGATGAAATGCTCAAAAGGGGGCTTCTACCGGACACATATACATATAGACCCTTAATAAGTGGTCTTTGTTCCACAAGTGGAGTGTCTGAAGCCAAAAAGTTTGTAGATGACCTACATAAGAAGAATTATAAGTTAAATGAGATGTGCTACAGTGCACTGCTGTATGGTTATTGCAAGGAAGGAAGATTATATGATGCATTAGGTGCCTGTCGTGACATGAGTGAAAGAGGAGTAGACATGGATCTAGTGTGTTATGCCATACTTATCTGTGGAGCTCTGAAACAACAGGATACCCGAAGATTATTTGGTCTCGTAAATGAGATGCATAGTCATGGATTGAGGCCTGATAATGTAATCTATACAAGTATGATTGATGCATACGGGAAAACAGGAAAACTTGATAAGGCATTTGGACTTTGGGATATAATGGTCGATGAAGGATGCTCTCCTAATGTTGTAACATACACAGCCTTGATCTATGGATTATGTAAGGCAGGCTGTATTGATAAAGCCGAGCTTCTTTGCAAGGATATGCTCGTGGGTGATACCCTTCCCAATCATGTCACATATGGTTGTTTCCTAGATCAATTCAGCAAAGAAGGTAGCATGGAGAAAGCTGTGCAACTACACAACACCATGCTTGCTGGGTTCTCAGCCAATACTGTAACATACAATATACTTATTAGGGGTTTCTGCAAAATGGGTAAGTTTCATGAAGCTTCAGAACTCTTGCTTGAGATGACAAATAATGGTGTTTACCCCGATTGCATCACCTATTCAACATTTATCTTTGAGTATTGTAGGATGGGCAATTTGCCAGAAGCTGTTAGGTTGTGGGATGTTATGCTAGATAGGGGTCTGAAGCCTGATATTCTAGCATACAACTTTTTGATACATGGTTGCTGTGTCACTGGAGAACTAACCAAGGCTTTTGAGTTGCGTGATGACATGGTGAGAAGAGGGCTGAAGCCAAATCAAGTTACATATAACACACTAAGTCGTGGAACTTGCCTTACTAGGTAA

Protein Analysis

477

Amino Acids

53.91

Weight (kDa)

6.64

Isoelectric Point (pI)

22.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 88 - 123 2.7e-10 PPR repeat family
PPR_long PF17177 93 - 199 3.9e-09 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 98 - 205 5.3e-10 Fungal tetratrico peptide repeats
PPR_3 PF13812 102 - 157 4.8e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 108 - 140 4.6e-15 PPR repeat
PPR_2 PF13041 112 - 160 1.2e-19 PPR repeat family
PPR PF01535 115 - 145 3.2e-10 PPR repeat
PPR_2 PF13041 183 - 228 1.3e-07 PPR repeat family
PPR_3 PF13812 241 - 296 3.1e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 249 - 281 6.7e-08 PPR repeat
PPR_2 PF13041 252 - 301 3.2e-18 PPR repeat family
PPR PF01535 255 - 285 1.2e-08 PPR repeat
PPR_long PF17177 261 - 352 8.4e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 284 - 315 2e-11 PPR repeat
PPR_2 PF13041 288 - 336 1.8e-09 PPR repeat family
PPR PF01535 290 - 316 6e-06 PPR repeat
PPR_3 PF13812 348 - 400 2.7e-06 Pentatricopeptide repeat domain
PPR_long PF17177 351 - 469 1.3e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 353 - 384 1.5e-11 PPR repeat
PPR_2 PF13041 357 - 404 2.3e-15 PPR repeat family
PPR PF01535 359 - 389 3.3e-09 PPR repeat
PPR_1 PF12854 387 - 419 2e-09 PPR repeat
PPR_2 PF13041 394 - 439 4e-12 PPR repeat family
PPR_3 PF13812 416 - 469 1.5e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 422 - 455 5.6e-08 PPR repeat
PPR_2 PF13041 426 - 474 4.8e-16 PPR repeat family
PPR PF01535 430 - 459 6.5e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59900
fragaria_vesca FvH4_3g35360
malus_domestica MD11G1144200.v1.1
prunus_persica Prupe.6G082100_v2.0.a1
pyrus_communis pycom11g09360
rosa_chinensis RchiOBHm_Chr5g0063471
rosa_laevigata RLG00000035637
rosa_multiflora Rmu_sc0016394.1_g000003
rosa_roxburghii Rroxscaffold_1G00017250
rosa_rugosa Rorug05G0357500
rosa_samantha Rh5AG416600 Rh5BG431800 Rh5CG455100 Rh5DG445300
rosa_wichuraiana Rw5G039140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 540
AatII GACGTC 1 cut(s) 144
AccB1I GGYRCC 1 cut(s) 614
AccI GTMKAC 1 cut(s) 645
AciI CCGC 1 cut(s) 17
AclWI GGATC 2 cut(s) 226, 660
AcsI RAATTY 1 cut(s) 299
AcuI CTGAAG 6 cut(s) 160, 309, 519, 1116, 1292, 1397
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 3 cut(s) 70, 350, 371
AgsI TTSAA 2 cut(s) 404, 1191
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 1 cut(s) 251
AluBI AGCT 9 cut(s) 7, 162, 198, 398, 686, 928, 1028, 1130, 1235
AluI AGCT 9 cut(s) 7, 162, 198, 398, 686, 928, 1028, 1130, 1235
Alw21I GWGCWC 3 cut(s) 200, 568, 688
Alw26I GTCTC 1 cut(s) 725
Alw44I GTGCAC 1 cut(s) 564
AlwI GGATC 2 cut(s) 226, 660
AlwNI CAGNNNCTG 1 cut(s) 1235
AoxI GGCC 1 cut(s) 752
ApaLI GTGCAC 1 cut(s) 564
ApoI RAATTY 1 cut(s) 299
AsuHPI GGTGA 4 cut(s) 355, 965, 1174, 1375
BaeGI GKGCMC 1 cut(s) 568
BanI GGYRCC 1 cut(s) 614
BanII GRGCYC 2 cut(s) 200, 688
BauI CACGAG 1 cut(s) 947
Bbv12I GWGCWC 3 cut(s) 200, 568, 688
BccI CCATC 3 cut(s) 277, 359, 1210
BciT130I CCWGG 1 cut(s) 253
BciVI GTATCC 1 cut(s) 694
BcoDI GTCTC 1 cut(s) 725
BfaI CTAG 6 cut(s) 393, 656, 992, 1259, 1286, 1428
BfmI CTRYAG 2 cut(s) 373, 559
BfuI GTATCC 1 cut(s) 694
Bme1390I CCNGG 1 cut(s) 253
BmgBI CACGTC 1 cut(s) 217
BmiI GGNNCC 2 cut(s) 155, 616
BmrFI CCNGG 1 cut(s) 253
BmsI GCATC 6 cut(s) 28, 595, 723, 775, 842, 1188
BpmI CTGGAG 2 cut(s) 184, 1344
BpuEI CTTGAG 1 cut(s) 1166
BsaHI GRCGYC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 725
BsaJI CCNNGG 2 cut(s) 251, 1335
BsaWI WCCGGW 1 cut(s) 439
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bse1I ACTGG 1 cut(s) 1327
BseBI CCWGG 1 cut(s) 253
BseDI CCNNGG 2 cut(s) 251, 1335
BseGI GGATG 3 cut(s) 857, 1221, 1255
BseMII CTCAG 2 cut(s) 79, 1077
BseNI ACTGG 1 cut(s) 1327
BseRI GAGGAG 1 cut(s) 654
BseSI GKGCMC 1 cut(s) 568
BseYI CCCAGC 1 cut(s) 1055
BshFI GGCC 1 cut(s) 754
BshNI GGYRCC 1 cut(s) 614
BsiHKAI GWGCWC 3 cut(s) 200, 568, 688
BsiSI CCGG 1 cut(s) 440
BslFI GGGAC 1 cut(s) 9
BsmAI GTCTC 1 cut(s) 725
BsmFI GGGAC 1 cut(s) 9
BsnI GGCC 1 cut(s) 754
Bso31I GGTCTC 1 cut(s) 725
Bsp1286I GDGCHC 3 cut(s) 200, 568, 688
Bsp143I GATC 4 cut(s) 231, 652, 885, 994
BspACI CCGC 1 cut(s) 17
BspANI GGCC 1 cut(s) 754
BspCNI CTCAG 2 cut(s) 78, 1076
BspHI TCATGA 1 cut(s) 1123
BspLI GGNNCC 2 cut(s) 155, 616
BspPI GGATC 2 cut(s) 226, 660
BspT107I GGYRCC 1 cut(s) 614
BspTNI GGTCTC 1 cut(s) 725
BsrI ACTGG 1 cut(s) 1327
BssECI CCNNGG 2 cut(s) 251, 1335
BssMI GATC 4 cut(s) 231, 652, 885, 994
BssNI GRCGYC 1 cut(s) 141
BssSI CACGAG 1 cut(s) 947
BssT1I CCWWGG 1 cut(s) 1335
Bst2BI CACGAG 1 cut(s) 947
Bst2UI CCWGG 1 cut(s) 253
Bst4CI ACNGT 3 cut(s) 374, 563, 1075
Bst6I CTCTTC 2 cut(s) 79, 1363
BstACI GRCGYC 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 614
BstC8I GCNNGC 3 cut(s) 277, 907, 1053
BstDEI CTNAG 3 cut(s) 65, 1063, 1406
BstF5I GGATG 3 cut(s) 857, 1221, 1255
BstKTI GATC 4 cut(s) 234, 655, 888, 997
BstMAI GTCTC 1 cut(s) 725
BstMBI GATC 4 cut(s) 231, 652, 885, 994
BstMWI GCNNNNNNNGC 1 cut(s) 614
BstNI CCWGG 1 cut(s) 253
BstSCI CCNGG 1 cut(s) 251
BstSFI CTRYAG 2 cut(s) 373, 559
BstSLI GKGCMC 1 cut(s) 568
BstX2I RGATCY 1 cut(s) 652
BstYI RGATCY 1 cut(s) 652
BsuI GTATCC 1 cut(s) 694
BsuRI GGCC 1 cut(s) 754
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 3 cut(s) 857, 1221, 1255
BtsI GCAGTG 1 cut(s) 566
BtsIMutI CAGTG 3 cut(s) 566, 568, 1320
Cac8I GCNNGC 3 cut(s) 277, 907, 1053
CaiI CAGNNNCTG 1 cut(s) 1235
CciI TCATGA 1 cut(s) 1123
Csp6I GTAC 3 cut(s) 69, 349, 370
CviQI GTAC 3 cut(s) 69, 349, 370
DdeI CTNAG 3 cut(s) 65, 1063, 1406
DpnI GATC 4 cut(s) 233, 654, 887, 996
DpnII GATC 4 cut(s) 231, 652, 885, 994
Eam1104I CTCTTC 2 cut(s) 79, 1363
EarI CTCTTC 2 cut(s) 79, 1363
Ecl136II GAGCTC 2 cut(s) 198, 686
Eco130I CCWWGG 1 cut(s) 1335
Eco147I AGGCCT 1 cut(s) 754
Eco24I GRGCYC 2 cut(s) 200, 688
Eco31I GGTCTC 1 cut(s) 725
Eco53kI GAGCTC 2 cut(s) 198, 686
Eco57I CTGAAG 6 cut(s) 160, 309, 519, 1116, 1292, 1397
EcoICRI GAGCTC 2 cut(s) 198, 686
EcoRII CCWGG 1 cut(s) 251
EcoT14I CCWWGG 1 cut(s) 1335
EcoT22I ATGCAT 3 cut(s) 610, 738, 790
EcoT38I GRGCYC 2 cut(s) 200, 688
ErhI CCWWGG 1 cut(s) 1335
FalI AAGNNNNNCTT 2 cut(s) 912, 944
FaqI GGGAC 1 cut(s) 9
FauNDI CATATG 1 cut(s) 979
FblI GTMKAC 1 cut(s) 645
FokI GGATG 3 cut(s) 864, 1228, 1262
FriOI GRGCYC 2 cut(s) 200, 688
FspBI CTAG 6 cut(s) 393, 656, 992, 1259, 1286, 1428
GsaI CCCAGC 1 cut(s) 1059
GsuI CTGGAG 2 cut(s) 184, 1344
HaeIII GGCC 1 cut(s) 754
HapII CCGG 1 cut(s) 440
Hin1I GRCGYC 1 cut(s) 141
HindIII AAGCTT 1 cut(s) 1128
HinfI GANTC 2 cut(s) 168, 247
HpaII CCGG 1 cut(s) 440
HphI GGTGA 4 cut(s) 355, 965, 1174, 1375
Hpy166II GTNNAC 3 cut(s) 566, 646, 1168
Hpy188I TCNGA 6 cut(s) 139, 231, 499, 690, 1135, 1272
Hpy188III TCNNGA 4 cut(s) 220, 235, 623, 1124
Hpy8I GTNNAC 3 cut(s) 566, 646, 1168
Hpy99I CGWCG 4 cut(s) 113, 143, 146, 221
HpyAV CCTTC 8 cut(s) 184, 196, 217, 374, 584, 842, 971, 1004
HpyCH4III ACNGT 3 cut(s) 374, 563, 1075
HpyCH4IV ACGT 4 cut(s) 141, 216, 240, 347
HpyF10VI GCNNNNNNNGC 1 cut(s) 614
HpyF3I CTNAG 3 cut(s) 65, 1063, 1406
HpySE526I ACGT 4 cut(s) 141, 216, 240, 347
Hsp92I GRCGYC 1 cut(s) 141
Kzo9I GATC 4 cut(s) 231, 652, 885, 994
LmnI GCTCC 1 cut(s) 683
LweI GCATC 6 cut(s) 28, 595, 723, 775, 842, 1188
MaeI CTAG 6 cut(s) 393, 656, 992, 1259, 1286, 1428
MaeII ACGT 4 cut(s) 141, 216, 240, 347
MaeIII GTNAC 8 cut(s) 343, 383, 623, 868, 973, 1075, 1318, 1390
MalI GATC 4 cut(s) 233, 654, 887, 996
MboI GATC 4 cut(s) 231, 652, 885, 994
MboII GAAGA 5 cut(s) 66, 544, 606, 720, 1380
MflI RGATCY 1 cut(s) 652
MhlI GDGCHC 3 cut(s) 200, 568, 688
MluCI AATT 6 cut(s) 79, 299, 404, 535, 998, 1222
MmeI TCCRAC 2 cut(s) 153, 162
MnlI CCTC 8 cut(s) 38, 48, 82, 143, 334, 632, 744, 1364
Mph1103I ATGCAT 3 cut(s) 610, 738, 790
MseI TTAA 3 cut(s) 203, 464, 545
MslI CAYNNNNRTG 1 cut(s) 741
MspI CCGG 1 cut(s) 440
MspR9I CCNGG 1 cut(s) 253
MvaI CCWGG 1 cut(s) 253
MwoI GCNNNNNNNGC 1 cut(s) 614
NdeI CATATG 1 cut(s) 979
NdeII GATC 4 cut(s) 231, 652, 885, 994
NlaIV GGNNCC 2 cut(s) 155, 616
NmeAIII GCCGAG 1 cut(s) 949
NmuCI GTSAC 4 cut(s) 343, 623, 973, 1318
NsiI ATGCAT 3 cut(s) 610, 738, 790
PagI TCATGA 1 cut(s) 1123
PceI AGGCCT 1 cut(s) 754
PcsI WCGNNNNNNNCGW 3 cut(s) 19, 108, 177
PfeI GAWTC 2 cut(s) 168, 247
PsiI TTATAA 1 cut(s) 540
Psp124BI GAGCTC 2 cut(s) 200, 688
Psp6I CCWGG 1 cut(s) 251
PspFI CCCAGC 1 cut(s) 1055
PspGI CCWGG 1 cut(s) 251
PspN4I GGNNCC 2 cut(s) 155, 616
PsrI GAACNNNNNNTAC 1 cut(s) 1408
PstNI CAGNNNCTG 1 cut(s) 1235
PsuI RGATCY 1 cut(s) 652
RsaI GTAC 3 cut(s) 70, 350, 371
RsaNI GTAC 3 cut(s) 69, 349, 370
RseI CAYNNNNRTG 1 cut(s) 741
SacI GAGCTC 2 cut(s) 200, 688
SaqAI TTAA 3 cut(s) 203, 464, 545
Sau3AI GATC 4 cut(s) 231, 652, 885, 994
ScrFI CCNGG 1 cut(s) 253
SduI GDGCHC 3 cut(s) 200, 568, 688
SfaNI GCATC 6 cut(s) 28, 595, 723, 775, 842, 1188
SfcI CTRYAG 2 cut(s) 373, 559
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 1 cut(s) 1145
SmoI CTYRAG 1 cut(s) 1145
Sse9I AATT 6 cut(s) 79, 299, 404, 535, 998, 1222
SseBI AGGCCT 1 cut(s) 754
SsiI CCGC 1 cut(s) 17
SspMI CTAG 6 cut(s) 393, 656, 992, 1259, 1286, 1428
SstI GAGCTC 2 cut(s) 200, 688
StuI AGGCCT 1 cut(s) 754
StyD4I CCNGG 1 cut(s) 251
StyI CCWWGG 1 cut(s) 1335
TaaI ACNGT 3 cut(s) 374, 563, 1075
TaiI ACGT 4 cut(s) 144, 219, 243, 350
TaqI TCGA 4 cut(s) 148, 194, 219, 843
TasI AATT 6 cut(s) 79, 299, 404, 535, 998, 1222
TfiI GAWTC 2 cut(s) 168, 247
Tru1I TTAA 3 cut(s) 203, 464, 545
Tru9I TTAA 3 cut(s) 203, 464, 545
TscAI CASTG 3 cut(s) 568, 573, 1327
TseFI GTSAC 4 cut(s) 343, 623, 973, 1318
Tsp45I GTSAC 4 cut(s) 343, 623, 973, 1318
TspDTI ATGAA 8 cut(s) 17, 112, 178, 324, 429, 861, 1112, 1140
TspRI CASTG 3 cut(s) 568, 573, 1327
VneI GTGCAC 1 cut(s) 564
XapI RAATTY 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 645
XspI CTAG 6 cut(s) 393, 656, 992, 1259, 1286, 1428
ZraI GACGTC 1 cut(s) 142
Zsp2I ATGCAT 3 cut(s) 610, 738, 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.