Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0016031

Physical Location & Seq

Genomic Coordinates Reverse (-)

69122869 .. 69125582

Gene Structure 2714 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G039140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2676 bp

ATGAAGCTCACTCGTCCGCATCGTCCCCTCCCAAACCCTCTCATTCACTCTAAACCCAGAAATCTCAGTACCTCTTCCAATTCCACCCCCAAAGATAATGACGAAAACGACGACCCCCACTTCATTTCCAACCTATCCGACGTCGTTCGAGGGAACCAGAGCTGGAGAATCGCCTTCAGCGACCCCTTCATTTCGAGCTCGTTAAAACCCCACCACGTCGAGAAGGTTCTGATCCAGAACGTAAGGAATCCCAGGTTAGCCTTGCGCTTCTTTAATTTTCTGGGCTTGCATAAATCCTTCAACCACTCAACGGCGTCGTTTTGCATTCTGATTCATGCCCTCGTTCAGTGTAGCCTCTTTTGGCCTGCCACCTCGCTTTTACAGACCCTCTTGCTTCGTGGGTTGAACCCAACTGAGGTTTTCCGGTCCCTTTTGAATTCATATAAGAAATTTAATTGCGCGACGAGTTTGGGTTTTGATTTGTTGGTGCAAAACTATTTGCAAAATAAGAGAGTGTTGGATGGTGTTGTGGTTGTGAGGCTGATGAGGGAGTGTAAAATGGTGCCTGAAGTTAGGACCTTGAATGCTCTTTTAAATGGGTTGGTGAGAATTAGGCATTTCAATATGGTTTTGCAGCTGTTTGATGAGTTGGTCAATGTTGGTCTTCGGCCGGATGCTTATATGTACACTGTGGTGGTTAGGAGTTTGTGTGAGTTGAAGGATTTGCACAAGGCTAATGAAGTGATTCGGTACGCGGAATCAAATGGGTGTGAGTTGAGTGTTGTTACGTATAATGTGTTGATTCATGGGCTCTGCAAGAGCCAGAGGGTTTGGGAGGCGGTAGAAATCAAGAATTCATTGAGTCGAAAGGGATTGAAAGCAGATATGGTTACTTATTGTACATTGGTGCTTGGGTTGTGTAAAGTGCAAGAATTTGAGGTTGGTGTGGAGCTGATGAAGGAAATGATTCGGCTGGGGTTTGTTCCAAGTGCAGCGGCTCTTTCGGGTCTTATGGAGGGATTGAGGAGGAAGGGGAAGATTGGAGATGCTTTTGATTTAGTGAACAGAATGGGAGAAGTTGGGGTGGTGCCTAATTTGTTTGCTTATAATGCATTGATCAATTCTTTGTGTAAAGACGGGAAGCTGGATGAGGCAGAATTACTCTTTGACAACATGGGGACGAAGGGTTTGTTTCCCAATGATGTTACATATTCTATTTTGATTGATTCATTCTGTCGAAGGGGAATATTGGACACTGCACATTGTTATTTTAATAAAATGATCAATGCTAGGATTAGATTAACTGTATATCCCTACAATTCTTTGATAAGTGGGGAGTGCAAGTTTGGAAAGTTGAGTGTGGCGGAATCTCTCTTCAGTGAGATGATGAGAAGAAGTGTGGAACCAACTGTTGTTACTTATACGTCACTGATAAGTGGATACTGTATAGGAGGAGAACTGCAGAAGGCATTTACACTGTATCATGAAATGATGGGAAGAGGCATTGCACCAAACACTTATACCTTCTCTGCAATTATTTCAGGTCTTTGTCGTGCAAGCATGATGGCTGAAGCAAGTAAATTCTTTGATGAAATGGTGGAAAGGGGCATTATGCCAAACGAGGTGACATACAATCTTTTGATTGATGGTTACTGTAGGGAAGGCAACATTACTAGAGCTTTTGAATTGCTTGATGAAATGCTCAAAAGGGGTCTTCTACCGGACACATATACATATAGACCTTTAATAAGTGGTCTCTGTTCCACGAGTGGAGTGTCTGAAGCCAAAAAGTTTGTAGATGACCTTCACAAGAAGAACTATAAGTTAAATGAGATGTGCTATAGTGCACTGCTGTATGGTTATTGCAAGGAAGGAAGATTATATGATGCATTAGGTGCCTGTCGTGACATGAGTGAAAGAGGAGTAGACATGGATCTAGTGTGTTATGCCATACTCATCTGTGGAGCTTTGAAACAACAGGACACCCGACAATTATTTGGTCTCGTAAACCAGATGCATAGTCAAGGATTGAGGCCTGATAATGTAATCTATACAAGTATGATTGATGCATACGGGAAAACAGGAAAGCTTGATAAGGCATTTGGACTTTGGGATATAATGGTTGGTGAAGGATGCTCTCCTAATGTTGTAACATACACGGCTTTGATCTATGGATTATGTAAGGCAGGCTGTATTGATAAAGCCGAGCTTCTTTGCAAGGATATGCTCGTCGGTGATGCCCTTCCCAATCATGTTACATATGGTTGTTTCCTAGATCACTTCAGCAAAGAAGGTAGCATGGAGAAAGCTGTGCAACTGCACAACACCATGCTTGCTGGGGGTTTCTGCAAAATGGGTAAGTTTCATGAAGCTTCAGAACTCTTGCTTGAGATGACAAACAATGGTGTTTACCCCGATTGCATCACCTATTCAACATTTATCTTTGAGTATTGTAGGATGGGCAATTTGCCGGAAGCTGTTAGGTTGTGGGATGTTATGCTAGATAGGGGTCTGAAGCCTGATATTCTAGCTTATAACTTTTTGATATATGGTTGCTGTGTCACTGGAGAACTAACCAAGGCTTTTGAGTTGCGTGATGACCTGATGAGAAGAGGGCTGAAGCCAAATCGAGTTACATATAACACACTAAGTCGTGGAACTGGCCTTACTAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

891

Amino Acids

100.29

Weight (kDa)

8.31

Isoelectric Point (pI)

26.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	189 - 237	3e-08	PPR repeat family
PPR_3	PF13812	213 - 269	4.1e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	228 - 273	1.6e-08	PPR repeat family
PPR_1	PF12854	255 - 283	5.3e-09	PPR repeat
PPR_2	PF13041	260 - 308	1.7e-12	PPR repeat family
PPR_long	PF17177	313 - 428	1.3e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	337 - 378	4.7e-08	PPR repeat family
PPR_1	PF12854	361 - 392	1.2e-11	PPR repeat
PPR_2	PF13041	364 - 413	2e-17	PPR repeat family
PPR	PF01535	368 - 397	1.8e-07	PPR repeat
PPR_1	PF12854	395 - 427	9e-09	PPR repeat
PPR_3	PF13812	425 - 479	1.8e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	438 - 482	2.4e-14	PPR repeat family
PPR	PF01535	439 - 466	3.5e-06	PPR repeat
PPR_3	PF13812	457 - 514	2.6e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	466 - 497	7.2e-10	PPR repeat
PPR_2	PF13041	469 - 518	1.5e-17	PPR repeat family
PPR	PF01535	472 - 502	4.1e-08	PPR repeat
PPR_long	PF17177	493 - 626	6.2e-10	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	500 - 533	3.5e-11	PPR repeat
PPR	PF01535	507 - 537	6.9e-07	PPR repeat
PPR_2	PF13041	511 - 553	1.8e-14	PPR repeat family
TPR_24	PF23276	525 - 632	1.1e-09	Fungal tetratrico peptide repeats
PPR_3	PF13812	529 - 584	1.1e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	535 - 567	9.8e-15	PPR repeat
PPR_2	PF13041	539 - 587	2.6e-19	PPR repeat family
PPR	PF01535	542 - 572	6.9e-10	PPR repeat
PPR_2	PF13041	610 - 655	2.9e-07	PPR repeat family
PPR_3	PF13812	668 - 723	2.7e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	676 - 708	1.4e-07	PPR repeat
PPR_2	PF13041	679 - 728	3.2e-17	PPR repeat family
PPR	PF01535	682 - 712	4.2e-08	PPR repeat
PPR_long	PF17177	699 - 781	3.2e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	711 - 742	4.3e-11	PPR repeat
PPR_2	PF13041	714 - 759	3.3e-09	PPR repeat family
PPR_2	PF13041	781 - 819	1.8e-08	PPR repeat family
PPR_1	PF12854	801 - 833	4.3e-09	PPR repeat
PPR_2	PF13041	808 - 853	4.2e-11	PPR repeat family
PPR_3	PF13812	830 - 883	8.5e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	840 - 886	9e-12	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016031)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G59900
fragaria_vesca	FvH4_3g35360
malus_domestica	MD11G1144200.v1.1
prunus_persica	Prupe.6G082100_v2.0.a1
pyrus_communis	pycom11g09360
rosa_chinensis	RchiOBHm_Chr5g0063471
rosa_laevigata	RLG00000035637
rosa_multiflora	Rmu_sc0016394.1_g000003
rosa_roxburghii	Rroxscaffold_1G00017250
rosa_rugosa	Rorug05G0357500
rosa_samantha	Rh5AG416600 Rh5BG431800 Rh5CG455100 Rh5DG445300
rosa_wichuraiana	Rw5G039140

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1107, 2535
AatII	GACGTC	1 cut(s)	144
AccB1I	GGYRCC	3 cut(s)	562, 1087, 1895
AccI	GTMKAC	1 cut(s)	1926
AccII	CGCG	2 cut(s)	461, 755
AciI	CCGC	5 cut(s)	17, 755, 839, 995, 1364
AclWI	GGATC	2 cut(s)	226, 1941
AcoI	YGGCCR	1 cut(s)	668
AcsI	RAATTY	5 cut(s)	436, 449, 853, 932, 1580
AcuI	CTGAAG	9 cut(s)	160, 588, 1360, 1590, 1800, 2266, 2358, 2534, 2639
AcyI	GRCGYC	2 cut(s)	141, 314
AfaI	GTAC	4 cut(s)	70, 686, 752, 901
AfiI	CCNNNNNNNGG	2 cut(s)	310, 415
AjiI	CACGTC	1 cut(s)	217
AjnI	CCWGG	1 cut(s)	251
AjuI	GAANNNNNNNTTGG	2 cut(s)	924, 956
AleI	CACNNNNGTG	1 cut(s)	692
Alw21I	GWGCWC	2 cut(s)	200, 1849
Alw26I	GTCTC	2 cut(s)	1760, 2006
Alw44I	GTGCAC	1 cut(s)	1845
AlwI	GGATC	2 cut(s)	226, 1941
AoxI	GGCC	4 cut(s)	362, 668, 2033, 2662
ApaLI	GTGCAC	1 cut(s)	1845
ApeKI	GCWGC	2 cut(s)	634, 992
ApoI	RAATTY	5 cut(s)	436, 449, 853, 932, 1580
ArsI	GACNNNNNNTTYG	2 cut(s)	1171, 1203
Asp700I	GAANNNNTTC	2 cut(s)	744, 966
AspLEI	GCGC	2 cut(s)	267, 461
AspS9I	GGNCC	2 cut(s)	426, 576
AsuHPI	GGTGA	5 cut(s)	616, 1636, 2138, 2246, 2416
AvaII	GGWCC	2 cut(s)	426, 576
BaeGI	GKGCMC	1 cut(s)	1849
BanI	GGYRCC	3 cut(s)	562, 1087, 1895
BanII	GRGCYC	2 cut(s)	200, 813
BarI	GAAGNNNNNNTAC	2 cut(s)	1788, 1820
BauI	CACGAG	1 cut(s)	1765
BbsI	GAAGAC	2 cut(s)	656, 1706
Bbv12I	GWGCWC	2 cut(s)	200, 1849
BbvI	GCAGC	2 cut(s)	646, 1004
BccI	CCATC	5 cut(s)	515, 1486, 1558, 1640, 2452
BceAI	ACGGC	2 cut(s)	327, 2175
BciT130I	CCWGG	1 cut(s)	253
BciVI	GTATCC	1 cut(s)	1433
BclI	TGATCA	2 cut(s)	1116, 1281
BcoDI	GTCTC	2 cut(s)	1760, 2006
BfaI	CTAG	7 cut(s)	1290, 1674, 1937, 2273, 2501, 2528, 2670
BfmI	CTRYAG	3 cut(s)	1460, 1654, 1840
BfuI	GTATCC	1 cut(s)	1433
BisI	GCNGC	3 cut(s)	635, 993, 996
BlsI	GCNGC	3 cut(s)	636, 994, 997
Bme1390I	CCNGG	1 cut(s)	253
Bme18I	GGWCC	2 cut(s)	426, 576
BmgBI	CACGTC	1 cut(s)	217
BmgT120I	GGNCC	2 cut(s)	426, 576
BmiI	GGNNCC	6 cut(s)	155, 428, 564, 1089, 1404, 1897
BmrFI	CCNGG	1 cut(s)	253
BmsI	GCATC	9 cut(s)	28, 664, 1036, 1876, 2004, 2056, 2123, 2227, 2430
BpiI	GAAGAC	2 cut(s)	656, 1706
BpmI	CTGGAG	2 cut(s)	184, 2586
BpuEI	CTTGAG	1 cut(s)	2408
BsaAI	YACGTR	1 cut(s)	789
BsaHI	GRCGYC	2 cut(s)	141, 314
BsaI	GGTCTC	2 cut(s)	1760, 2006
BsaJI	CCNNGG	2 cut(s)	251, 2577
BsaWI	WCCGGW	2 cut(s)	423, 1720
BsaXI	ACNNNNNCTCC	4 cut(s)	694, 724, 1328, 1358
Bsc4I	CCNNNNNNNGG	2 cut(s)	310, 415
Bse1I	ACTGG	2 cut(s)	2569, 2665
Bse3DI	GCAATG	1 cut(s)	1503
BseBI	CCWGG	1 cut(s)	253
BseDI	CCNNGG	2 cut(s)	251, 2577
BseGI	GGATG	6 cut(s)	526, 679, 1153, 2138, 2463, 2497
BseLI	CCNNNNNNNGG	2 cut(s)	310, 415
BseMI	GCAATG	1 cut(s)	1503
BseMII	CTCAG	2 cut(s)	79, 405
BseNI	ACTGG	2 cut(s)	2569, 2665
BseRI	GAGGAG	3 cut(s)	1039, 1467, 1935
BseSI	GKGCMC	1 cut(s)	1849
BseX3I	CGGCCG	1 cut(s)	668
BseXI	GCAGC	2 cut(s)	646, 1004
BseYI	CCCAGC	2 cut(s)	973, 2336
BsgI	GTGCAG	3 cut(s)	1011, 1242, 2303
Bsh1236I	CGCG	2 cut(s)	461, 755
Bsh1285I	CGRYCG	1 cut(s)	671
BshFI	GGCC	4 cut(s)	364, 670, 2035, 2664
BshNI	GGYRCC	3 cut(s)	562, 1087, 1895
BsiEI	CGRYCG	1 cut(s)	671
BsiHKAI	GWGCWC	2 cut(s)	200, 1849
BsiSI	CCGG	4 cut(s)	424, 671, 1721, 2471
BslFI	GGGAC	3 cut(s)	9, 412, 1192
BslI	CCNNNNNNNGG	2 cut(s)	310, 415
BsmAI	GTCTC	2 cut(s)	1760, 2006
BsmFI	GGGAC	3 cut(s)	9, 412, 1192
BsmI	GAATGC	2 cut(s)	324, 589
BsnI	GGCC	4 cut(s)	364, 670, 2035, 2664
Bso31I	GGTCTC	2 cut(s)	1760, 2006
Bsp1286I	GDGCHC	3 cut(s)	200, 813, 1849
Bsp1407I	TGTACA	2 cut(s)	684, 899
Bsp143I	GATC	6 cut(s)	231, 1116, 1281, 1933, 2166, 2275
BspACI	CCGC	5 cut(s)	17, 755, 839, 995, 1364
BspANI	GGCC	4 cut(s)	364, 670, 2035, 2664
BspCNI	CTCAG	2 cut(s)	78, 406
BspFNI	CGCG	2 cut(s)	461, 755
BspHI	TCATGA	2 cut(s)	1483, 2365
BspLI	GGNNCC	6 cut(s)	155, 428, 564, 1089, 1404, 1897
BspMAI	CTGCAG	1 cut(s)	1464
BspPI	GGATC	2 cut(s)	226, 1941
BspT107I	GGYRCC	3 cut(s)	562, 1087, 1895
BspTNI	GGTCTC	2 cut(s)	1760, 2006
BsrDI	GCAATG	1 cut(s)	1503
BsrGI	TGTACA	2 cut(s)	684, 899
BsrI	ACTGG	2 cut(s)	2569, 2665
BssECI	CCNNGG	2 cut(s)	251, 2577
BssMI	GATC	6 cut(s)	231, 1116, 1281, 1933, 2166, 2275
BssNI	GRCGYC	2 cut(s)	141, 314
BssSI	CACGAG	1 cut(s)	1765
BssT1I	CCWWGG	1 cut(s)	2577
Bst2BI	CACGAG	1 cut(s)	1765
Bst2UI	CCWGG	1 cut(s)	253
Bst4CI	ACNGT	6 cut(s)	691, 1306, 1411, 1445, 1479, 1655
Bst6I	CTCTTC	4 cut(s)	79, 1379, 1492, 2605
BstACI	GRCGYC	2 cut(s)	141, 314
BstAPI	GCANNNNNTGC	1 cut(s)	1895
BstAUI	TGTACA	2 cut(s)	684, 899
BstBAI	YACGTR	1 cut(s)	789
BstC8I	GCNNGC	5 cut(s)	287, 366, 1558, 2188, 2334
BstDEI	CTNAG	3 cut(s)	65, 414, 2648
BstF5I	GGATG	6 cut(s)	526, 679, 1153, 2138, 2463, 2497
BstFNI	CGCG	2 cut(s)	461, 755
BstHHI	GCGC	2 cut(s)	267, 461
BstKTI	GATC	6 cut(s)	234, 1119, 1284, 1936, 2169, 2278
BstMAI	GTCTC	2 cut(s)	1760, 2006
BstMBI	GATC	6 cut(s)	231, 1116, 1281, 1933, 2166, 2275
BstMCI	CGRYCG	1 cut(s)	671
BstMWI	GCNNNNNNNGC	2 cut(s)	1109, 1895
BstNI	CCWGG	1 cut(s)	253
BstSCI	CCNGG	1 cut(s)	251
BstSFI	CTRYAG	3 cut(s)	1460, 1654, 1840
BstSLI	GKGCMC	1 cut(s)	1849
BstSNI	TACGTA	1 cut(s)	789
BstUI	CGCG	2 cut(s)	461, 755
BstV1I	GCAGC	2 cut(s)	646, 1004
BstV2I	GAAGAC	2 cut(s)	656, 1706
BstX2I	RGATCY	1 cut(s)	1933
BstYI	RGATCY	1 cut(s)	1933
BstZI	CGGCCG	1 cut(s)	668
BsuI	GTATCC	1 cut(s)	1433
BsuRI	GGCC	4 cut(s)	364, 670, 2035, 2664
BtrI	CACGTC	1 cut(s)	217
BtsCI	GGATG	6 cut(s)	526, 679, 1153, 2138, 2463, 2497
BtsI	GCAGTG	2 cut(s)	1254, 1847
BtsIMutI	CAGTG	8 cut(s)	353, 687, 1254, 1384, 1427, 1475, 1847, 2562
Cac8I	GCNNGC	5 cut(s)	287, 366, 1558, 2188, 2334
CciI	TCATGA	2 cut(s)	1483, 2365
CfoI	GCGC	2 cut(s)	267, 461
Cfr13I	GGNCC	2 cut(s)	426, 576
CseI	GACGC	1 cut(s)	303
Csp6I	GTAC	4 cut(s)	69, 685, 751, 900
CviQI	GTAC	4 cut(s)	69, 685, 751, 900
DdeI	CTNAG	3 cut(s)	65, 414, 2648
DpnI	GATC	6 cut(s)	233, 1118, 1283, 1935, 2168, 2277
DpnII	GATC	6 cut(s)	231, 1116, 1281, 1933, 2166, 2275
DraI	TTTAAA	1 cut(s)	594
EaeI	YGGCCR	1 cut(s)	668
EagI	CGGCCG	1 cut(s)	668
Eam1104I	CTCTTC	4 cut(s)	79, 1379, 1492, 2605
EarI	CTCTTC	4 cut(s)	79, 1379, 1492, 2605
EciI	GGCGGA	1 cut(s)	1379
Ecl136II	GAGCTC	1 cut(s)	198
EclXI	CGGCCG	1 cut(s)	668
Eco105I	TACGTA	1 cut(s)	789
Eco130I	CCWWGG	1 cut(s)	2577
Eco147I	AGGCCT	1 cut(s)	2035
Eco24I	GRGCYC	2 cut(s)	200, 813
Eco31I	GGTCTC	2 cut(s)	1760, 2006
Eco47I	GGWCC	2 cut(s)	426, 576
Eco52I	CGGCCG	1 cut(s)	668
Eco53kI	GAGCTC	1 cut(s)	198
Eco57I	CTGAAG	9 cut(s)	160, 588, 1360, 1590, 1800, 2266, 2358, 2534, 2639
EcoICRI	GAGCTC	1 cut(s)	198
EcoO109I	RGGNCCY	1 cut(s)	576
EcoRI	GAATTC	2 cut(s)	436, 853
EcoRII	CCWGG	1 cut(s)	251
EcoT14I	CCWWGG	1 cut(s)	2577
EcoT22I	ATGCAT	4 cut(s)	1114, 1891, 2019, 2071
EcoT38I	GRGCYC	2 cut(s)	200, 813
ErhI	CCWWGG	1 cut(s)	2577
FalI	AAGNNNNNCTT	2 cut(s)	2193, 2225
FaqI	GGGAC	3 cut(s)	9, 412, 1192
FauNDI	CATATG	1 cut(s)	2260
FbaI	TGATCA	2 cut(s)	1116, 1281
FblI	GTMKAC	1 cut(s)	1926
Fnu4HI	GCNGC	3 cut(s)	635, 993, 996
FokI	GGATG	6 cut(s)	533, 686, 1160, 2145, 2470, 2504
FriOI	GRGCYC	2 cut(s)	200, 813
Fsp4HI	GCNGC	3 cut(s)	635, 993, 996
FspBI	CTAG	7 cut(s)	1290, 1674, 1937, 2273, 2501, 2528, 2670
GlaI	GCGC	2 cut(s)	266, 460
GluI	GCNGC	3 cut(s)	635, 993, 996
GsaI	CCCAGC	2 cut(s)	977, 2340
GsuI	CTGGAG	2 cut(s)	184, 2586
HaeIII	GGCC	4 cut(s)	364, 670, 2035, 2664
HapII	CCGG	4 cut(s)	424, 671, 1721, 2471
HgaI	GACGC	1 cut(s)	303
HhaI	GCGC	2 cut(s)	267, 461
Hin1I	GRCGYC	2 cut(s)	141, 314
Hin6I	GCGC	2 cut(s)	265, 459
HinP1I	GCGC	2 cut(s)	265, 459
HindIII	AAGCTT	2 cut(s)	2087, 2370
HpaII	CCGG	4 cut(s)	424, 671, 1721, 2471
HphI	GGTGA	5 cut(s)	616, 1636, 2138, 2246, 2416
Hpy166II	GTNNAC	6 cut(s)	687, 1063, 1847, 1927, 2008, 2410
Hpy188I	TCNGA	6 cut(s)	139, 231, 330, 1780, 2377, 2514
Hpy188III	TCNNGA	6 cut(s)	220, 235, 850, 1484, 1904, 2366
Hpy8I	GTNNAC	6 cut(s)	687, 1063, 1847, 1927, 2008, 2410
Hpy99I	CGWCG	7 cut(s)	113, 143, 146, 221, 319, 466, 2234
HpyCH4III	ACNGT	6 cut(s)	691, 1306, 1411, 1445, 1479, 1655
HpyCH4IV	ACGT	5 cut(s)	141, 216, 240, 788, 1424
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1109, 1895
HpyF3I	CTNAG	3 cut(s)	65, 414, 2648
HpySE526I	ACGT	5 cut(s)	141, 216, 240, 788, 1424
Hsp92I	GRCGYC	2 cut(s)	141, 314
HspAI	GCGC	2 cut(s)	265, 459
Ksp22I	TGATCA	2 cut(s)	1116, 1281
Kzo9I	GATC	6 cut(s)	231, 1116, 1281, 1933, 2166, 2275
LmnI	GCTCC	2 cut(s)	949, 1964
Lsp1109I	GCAGC	2 cut(s)	646, 1004
LweI	GCATC	9 cut(s)	28, 664, 1036, 1876, 2004, 2056, 2123, 2227, 2430
MaeI	CTAG	7 cut(s)	1290, 1674, 1937, 2273, 2501, 2528, 2670
MaeII	ACGT	5 cut(s)	141, 216, 240, 788, 1424
MalI	GATC	6 cut(s)	233, 1118, 1283, 1935, 2168, 2277
MboI	GATC	6 cut(s)	231, 1116, 1281, 1933, 2166, 2275
MflI	RGATCY	1 cut(s)	1933
MhlI	GDGCHC	3 cut(s)	200, 813, 1849
MlyI	GAGTC	1 cut(s)	871
MmeI	TCCRAC	3 cut(s)	153, 162, 498
Mph1103I	ATGCAT	4 cut(s)	1114, 1891, 2019, 2071
MroXI	GAANNNNTTC	2 cut(s)	744, 966
MseI	TTAA	8 cut(s)	203, 273, 453, 593, 1272, 1301, 1745, 1826
MslI	CAYNNNNRTG	1 cut(s)	692
MspA1I	CMGCKG	2 cut(s)	637, 995
MspI	CCGG	4 cut(s)	424, 671, 1721, 2471
MspR9I	CCNGG	1 cut(s)	253
Mva1269I	GAATGC	2 cut(s)	324, 589
MvaI	CCWGG	1 cut(s)	253
MvnI	CGCG	2 cut(s)	461, 755
MwoI	GCNNNNNNNGC	2 cut(s)	1109, 1895
NdeI	CATATG	1 cut(s)	2260
NdeII	GATC	6 cut(s)	231, 1116, 1281, 1933, 2166, 2275
NlaIV	GGNNCC	6 cut(s)	155, 428, 564, 1089, 1404, 1897
NmeAIII	GCCGAG	1 cut(s)	2230
NmuCI	GTSAC	4 cut(s)	1425, 1624, 1904, 2560
NsiI	ATGCAT	4 cut(s)	1114, 1891, 2019, 2071
OliI	CACNNNNGTG	1 cut(s)	692
PagI	TCATGA	2 cut(s)	1483, 2365
PceI	AGGCCT	1 cut(s)	2035
PcsI	WCGNNNNNNNCGW	3 cut(s)	19, 108, 177
PctI	GAATGC	2 cut(s)	324, 589
PdmI	GAANNNNTTC	2 cut(s)	744, 966
PfeI	GAWTC	9 cut(s)	168, 247, 331, 745, 758, 802, 967, 1226, 1367
PkrI	GCNGC	3 cut(s)	636, 994, 997
PleI	GAGTC	1 cut(s)	870
PpsI	GAGTC	1 cut(s)	870
Ppu21I	YACGTR	1 cut(s)	789
PpuMI	RGGWCCY	1 cut(s)	576
PsiI	TTATAA	2 cut(s)	1107, 2535
Psp124BI	GAGCTC	1 cut(s)	200
Psp5II	RGGWCCY	1 cut(s)	576
Psp6I	CCWGG	1 cut(s)	251
PspFI	CCCAGC	2 cut(s)	973, 2336
PspGI	CCWGG	1 cut(s)	251
PspN4I	GGNNCC	6 cut(s)	155, 428, 564, 1089, 1404, 1897
PspPI	GGNCC	2 cut(s)	426, 576
PspPPI	RGGWCCY	1 cut(s)	576
PsrI	GAACNNNNNNTAC	1 cut(s)	2650
PstI	CTGCAG	1 cut(s)	1464
PsuI	RGATCY	1 cut(s)	1933
PvuII	CAGCTG	1 cut(s)	637
RsaI	GTAC	4 cut(s)	70, 686, 752, 901
RsaNI	GTAC	4 cut(s)	69, 685, 751, 900
RseI	CAYNNNNRTG	1 cut(s)	692
SacI	GAGCTC	1 cut(s)	200
SaqAI	TTAA	8 cut(s)	203, 273, 453, 593, 1272, 1301, 1745, 1826
SatI	GCNGC	3 cut(s)	635, 993, 996
Sau3AI	GATC	6 cut(s)	231, 1116, 1281, 1933, 2166, 2275
Sau96I	GGNCC	2 cut(s)	426, 576
SchI	GAGTC	1 cut(s)	871
ScrFI	CCNGG	1 cut(s)	253
SduI	GDGCHC	3 cut(s)	200, 813, 1849
SfaNI	GCATC	9 cut(s)	28, 664, 1036, 1876, 2004, 2056, 2123, 2227, 2430
SfcI	CTRYAG	3 cut(s)	1460, 1654, 1840
SinI	GGWCC	2 cut(s)	426, 576
SmiMI	CAYNNNNRTG	1 cut(s)	692
SmlI	CTYRAG	1 cut(s)	2387
SmoI	CTYRAG	1 cut(s)	2387
SnaBI	TACGTA	1 cut(s)	789
SseBI	AGGCCT	1 cut(s)	2035
SsiI	CCGC	5 cut(s)	17, 755, 839, 995, 1364
SspI	AATATT	1 cut(s)	1248
SspMI	CTAG	7 cut(s)	1290, 1674, 1937, 2273, 2501, 2528, 2670
SstI	GAGCTC	1 cut(s)	200
StuI	AGGCCT	1 cut(s)	2035
StyD4I	CCNGG	1 cut(s)	251
StyI	CCWWGG	1 cut(s)	2577
TaaI	ACNGT	6 cut(s)	691, 1306, 1411, 1445, 1479, 1655
TaiI	ACGT	5 cut(s)	144, 219, 243, 791, 1427
TaqI	TCGA	6 cut(s)	148, 194, 219, 865, 1237, 2629
TatI	WGTACW	2 cut(s)	684, 899
TauI	GCSGC	1 cut(s)	998
TfiI	GAWTC	9 cut(s)	168, 247, 331, 745, 758, 802, 967, 1226, 1367
Tru1I	TTAA	8 cut(s)	203, 273, 453, 593, 1272, 1301, 1745, 1826
Tru9I	TTAA	8 cut(s)	203, 273, 453, 593, 1272, 1301, 1745, 1826
TscAI	CASTG	8 cut(s)	353, 694, 1261, 1384, 1434, 1482, 1854, 2569
TseFI	GTSAC	4 cut(s)	1425, 1624, 1904, 2560
TseI	GCWGC	2 cut(s)	634, 992
Tsp45I	GTSAC	4 cut(s)	1425, 1624, 1904, 2560
TspRI	CASTG	8 cut(s)	353, 694, 1261, 1384, 1434, 1482, 1854, 2569
VneI	GTGCAC	1 cut(s)	1845
VpaK11BI	GGWCC	2 cut(s)	426, 576
XapI	RAATTY	5 cut(s)	436, 449, 853, 932, 1580
XmiI	GTMKAC	1 cut(s)	1926
XmnI	GAANNNNTTC	2 cut(s)	744, 966
XspI	CTAG	7 cut(s)	1290, 1674, 1937, 2273, 2501, 2528, 2670
ZraI	GACGTC	1 cut(s)	142
Zsp2I	ATGCAT	4 cut(s)	1114, 1891, 2019, 2071

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.