Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0016031

Physical Location & Seq

Genomic Coordinates Reverse (-)

71852717 .. 71856082

Gene Structure 3366 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG416600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2748 bp

ATGGGTCGATCCTCTTTCATTTCAACCCCAAAAATGAAGCTCACTCGTCCGCATCGTCCCCTCCCAAACCCTCTCATTCACTCTAAACCCAGAAATCTCAGTACCTCTTCCAATTCAACCCCCAAAGATAATGACGAAAACGACGACCCCCACTTCATTTCCAACCTATCCGACGTCGTTCGAGGGAACCAGAGCTGGAGAATCGCCTTCAGCGACCCCTTCATTTCGAGCTCGTTAAAACCCCACCACGTCGAGAAGGTTCTGATCCAGAACGTAAGGAATCCCAGGTTAGCCTTGCGCTTCTTTAATTTTCTGGGCTTGCATAAATCCTTCAACCACTCAACGGCGTCGTTTTGCATTCTGATTCATGCCCTCGTTCAGTGTAGCCTCTTTTGGCCTGCCACCTCGCTTTTACAGACCCTCTTGCTTCGTGGGTTGAACCCAACTGAGGTTTTCCGGTCCCTTTTGAATTCATATAAGAAATTTAATTGCGCGACGAGTTTGGGTTTTGATTTGTTGGTGCAAAACTATGTGCAAAATAAGAGAGTGTTGGATGGTGTTGTGGTTGTGAGGCTGATGAGGGAGTGTAAAATGGAGCCTGAAGTTAGAACCTTGAATGCTCTTTTAAATGGGTTGGTGAGAATTAGGCATTTCAATATGGTTTTGCAGCTGTTTGATGAGTTGGTCAATGTTGGTCTTCGGCCGGATGCTTATATGTACACTGTGGTGGTTAGGAGTTTGTGTGAGTTGAAGGATTTGCACAAGGCTAATGAAGTGATTCGGTACGCGGAATCAAATGGGTGTGAGTTGAGTGTTGTTACGTATAATGTGTTGATTCATGGACTCTGCAAGAGCCAGAGAGTTTGGGAGGCGGTAGAAATCAAGAATTTATTGAGTCGAAAGGGATTGAAAGCAGATATGGTTACTTATTGTACATTGGTGCTTGGGTTGTGTAAAGTGCAAGAATTTGAGGCTGGTGTGGAGCTAATGAAGGAAATGATTCGGCTGGGGTTTGTTCCAAGTGCAGCGGCTCTTTCGGGTCTTATGGAGGGATTGAGGAGGAAGGGGAAGATTGGAGATGCTTTTGATTTAGTGAACAGAATGGGAGAAGTTGGGGTGGTGCCTAATTTGTTTGCTTATAATGCATTGATCAATTCTTTGTGTAAAGACGGGAAGCTGGATGAGGCAGAATTACTCTTTGACAACATGGGGACGAAGGGTTTGTTTCCCAATGATGTTACATATTCTATTTTGATTGATTCATTCTGTCGAAGGGGAATATTGGACACTGCACATTGTTATTTTAATAAAATGATCAATGCAAGGATTAGATTAACTGTATATCCCTACAATTCTTTGATAAGTGGGGAGTGCAAGTTTGGAAAGTTGAGTGTGGCGGAATCTCTCTTCAGTGAGATGATGAGTAGAAGTGTGGAACCAACTGTCGTTACTTATACGTCACTGATAAGTGGATACTGTAAAGGAGGAGAAATGCAGAAGGCATTCATACTGTATCATGAAATGATGGGAAGAGGCATTGCACCAAATACTTATACCTCCTCTGCAATTATTTCAGGTCTTTGTCGTGCAAGCATGATGGCTGAAGCAAGTAAATTCTTTGATGAAATGGTGGAAAGGGGCATTATGCCAAACGAGGTGACATACAATCTTTTGATTGATGGGTACTGTAGGGAAGGTAACATTACTAGAGCTTTTGAATTGCTTGATGAAATGCTCAAAAGGGGGCTTCTACCAGACACATATACATATAGACCCTTAATAAGTGGTCTTTGTTCCACAAGTGGAGTGTCTGAAGCCAAAAAGTTTGTAGATGACCTACACAAGAAGAATTATAAGTTAAATGAGATGTGCTACAGTGCACTGCTGTATGGTTATTGCAAGGAAGGAAGATTATATGATGCATTAGGTGCCTGTCGTGACATGAGTGAAAGAGGAGTAGACATGGATCTAGTGTGTTATGCCATACTTATCTGTGGAGCTCTGAAACAACAGGATACCCGAAGATTATTTGGTCTCGTAAATGAGATGCATAGTCATGGATTGAGGCCTGATAATGTAATCTATACAAGTATGATCGATGCATATGGGAAAACAGGAAAACTTGATAAGGCATTTGGACTTTGGGATATAATGGTTGATGAAGGATGCTCTCCCAATGTTGTAACATACACAGCCTTGATCTATGGATTATGTAAGGCAGGCTGTATTGATAAAGCCGAGCTTCTTTGCAAGGATATGCTCGTGGGTGATGCCCTTCCCAATCATGTCACATATGGTTGTTTCCTAGATCACTTCAGCAAAGAAGGTAGCATGGAGAAAGCTGTGCAACTACACAACACCATGCTTGCTGGGTTCTCAGCCAATACTGTAACATACAATATACTTATTAGGGGTTTCTGCAAAATGAGTAAGTTTCATGAAGCTTCAGAACTCTTGCTTGAGATGACAAATAATGGTGTTTACCCCGATTGCATCACATATTCAACATTTATCTTTGAGTATTGTAGGATGGGCAATTTGCCAGAAGCTGTTAGGTTGTGGGATGTTATGCTAGATAGGGGTCTGAAGCCTGATATTCTAGCATATAACTTTTTGATATATGGTTGCTGTGTCACTGGAGAACTAACCAAGGCTTTTGAGTTGCGTGATGACATGATGAGAAGAGGGCTGAAGCCAAATCGAGTTACATATAACACACTAAGTCGTGGAACTTGCCTTACTAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

915

Amino Acids

103.1

Weight (kDa)

8.36

Isoelectric Point (pI)

28.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	200 - 248	3.1e-08	PPR repeat family
PPR_3	PF13812	224 - 280	4.2e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	240 - 284	2.1e-08	PPR repeat family
PPR_1	PF12854	266 - 294	6.1e-09	PPR repeat
PPR_2	PF13041	271 - 319	5.8e-12	PPR repeat family
PPR_long	PF17177	323 - 439	8.3e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	372 - 403	1.3e-11	PPR repeat
PPR_2	PF13041	375 - 424	2.1e-17	PPR repeat family
PPR	PF01535	379 - 408	1.9e-07	PPR repeat
PPR_1	PF12854	406 - 438	9.2e-09	PPR repeat
PPR_3	PF13812	436 - 492	1.9e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	449 - 494	1.2e-14	PPR repeat family
PPR	PF01535	450 - 477	5.6e-06	PPR repeat
PPR_3	PF13812	468 - 525	4.1e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	477 - 508	2.3e-10	PPR repeat
PPR_2	PF13041	480 - 529	1.3e-17	PPR repeat family
PPR	PF01535	483 - 513	4.8e-09	PPR repeat
PPR_1	PF12854	511 - 544	2.6e-08	PPR repeat
PPR	PF01535	519 - 548	1e-05	PPR repeat
PPR_2	PF13041	522 - 564	1.4e-14	PPR repeat family
PPR_long	PF17177	532 - 637	1.5e-08	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	536 - 644	8.3e-10	Fungal tetratrico peptide repeats
PPR_3	PF13812	540 - 595	1.1e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	546 - 578	1e-14	PPR repeat
PPR_2	PF13041	550 - 598	2.7e-19	PPR repeat family
PPR	PF01535	553 - 583	7.1e-10	PPR repeat
PPR_2	PF13041	621 - 666	3e-07	PPR repeat family
PPR_3	PF13812	679 - 734	7.3e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	687 - 719	1.5e-07	PPR repeat
PPR_2	PF13041	690 - 739	7.2e-18	PPR repeat family
PPR	PF01535	693 - 723	2.6e-08	PPR repeat
PPR_1	PF12854	722 - 753	4.4e-11	PPR repeat
PPR_2	PF13041	725 - 771	2e-09	PPR repeat family
PPR_2	PF13041	760 - 808	2e-12	PPR repeat family
PPR_1	PF12854	791 - 822	1.7e-09	PPR repeat
PPR_2	PF13041	795 - 843	1.7e-14	PPR repeat family
PPR	PF01535	797 - 827	1.5e-07	PPR repeat
PPR_1	PF12854	825 - 857	4.4e-09	PPR repeat
PPR_2	PF13041	834 - 877	6.7e-10	PPR repeat family
PPR_3	PF13812	854 - 907	5.4e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	860 - 892	1.6e-06	PPR repeat
PPR_2	PF13041	864 - 912	3.4e-14	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016031)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G59900
fragaria_vesca	FvH4_3g35360
malus_domestica	MD11G1144200.v1.1
prunus_persica	Prupe.6G082100_v2.0.a1
pyrus_communis	pycom11g09360
rosa_chinensis	RchiOBHm_Chr5g0063471
rosa_laevigata	RLG00000035637
rosa_multiflora	Rmu_sc0016394.1_g000003
rosa_roxburghii	Rroxscaffold_1G00017250
rosa_rugosa	Rorug05G0357500
rosa_samantha	Rh5AG416600 Rh5BG431800 Rh5CG455100 Rh5DG445300
rosa_wichuraiana	Rw5G039140

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1140, 1854
AatII	GACGTC	1 cut(s)	177
AccB1I	GGYRCC	2 cut(s)	1120, 1928
AccI	GTMKAC	1 cut(s)	1959
AccII	CGCG	2 cut(s)	494, 788
AciI	CCGC	5 cut(s)	50, 788, 872, 1028, 1397
AclWI	GGATC	3 cut(s)	3, 259, 1974
AcoI	YGGCCR	1 cut(s)	701
AcsI	RAATTY	5 cut(s)	469, 482, 886, 965, 1613
AcuI	CTGAAG	9 cut(s)	193, 621, 1393, 1623, 1833, 2299, 2430, 2606, 2711
AcyI	GRCGYC	2 cut(s)	174, 347
AfaI	GTAC	5 cut(s)	103, 719, 785, 934, 1685
AfiI	CCNNNNNNNGG	2 cut(s)	343, 448
AjiI	CACGTC	1 cut(s)	250
AjnI	CCWGG	1 cut(s)	284
AleI	CACNNNNGTG	1 cut(s)	725
Alw21I	GWGCWC	3 cut(s)	233, 1882, 2002
Alw26I	GTCTC	1 cut(s)	2039
Alw44I	GTGCAC	1 cut(s)	1878
AlwI	GGATC	3 cut(s)	3, 259, 1974
AlwNI	CAGNNNCTG	1 cut(s)	2549
AoxI	GGCC	3 cut(s)	395, 701, 2066
ApaLI	GTGCAC	1 cut(s)	1878
ApeKI	GCWGC	2 cut(s)	667, 1025
ApoI	RAATTY	5 cut(s)	469, 482, 886, 965, 1613
ArsI	GACNNNNNNTTYG	2 cut(s)	1204, 1236
Asp700I	GAANNNNTTC	3 cut(s)	777, 999, 1502
AspLEI	GCGC	2 cut(s)	300, 494
AspS9I	GGNCC	1 cut(s)	459
AsuHPI	GGTGA	3 cut(s)	649, 1669, 2279
AvaII	GGWCC	1 cut(s)	459
BaeGI	GKGCMC	1 cut(s)	1882
BanI	GGYRCC	2 cut(s)	1120, 1928
BanII	GRGCYC	2 cut(s)	233, 2002
BauI	CACGAG	1 cut(s)	2261
BbsI	GAAGAC	1 cut(s)	689
Bbv12I	GWGCWC	3 cut(s)	233, 1882, 2002
BbvI	GCAGC	2 cut(s)	679, 1037
BccI	CCATC	5 cut(s)	548, 1519, 1591, 1673, 2524
BceAI	ACGGC	1 cut(s)	360
BciT130I	CCWGG	1 cut(s)	286
BciVI	GTATCC	2 cut(s)	1466, 2008
BclI	TGATCA	2 cut(s)	1149, 1314
BcoDI	GTCTC	1 cut(s)	2039
BfaI	CTAG	6 cut(s)	1707, 1970, 2306, 2573, 2600, 2742
BfmI	CTRYAG	2 cut(s)	1687, 1873
BfuI	GTATCC	2 cut(s)	1466, 2008
BisI	GCNGC	3 cut(s)	668, 1026, 1029
BlsI	GCNGC	3 cut(s)	669, 1027, 1030
Bme1390I	CCNGG	1 cut(s)	286
Bme18I	GGWCC	1 cut(s)	459
BmgBI	CACGTC	1 cut(s)	250
BmgT120I	GGNCC	1 cut(s)	459
BmiI	GGNNCC	6 cut(s)	188, 461, 597, 1122, 1437, 1930
BmrFI	CCNGG	1 cut(s)	286
BmsI	GCATC	9 cut(s)	61, 697, 1069, 1909, 2037, 2089, 2156, 2260, 2502
BpiI	GAAGAC	1 cut(s)	689
BpmI	CTGGAG	2 cut(s)	217, 2658
BpuEI	CTTGAG	1 cut(s)	2480
Bsa29I	ATCGAT	1 cut(s)	2097
BsaAI	YACGTR	1 cut(s)	822
BsaHI	GRCGYC	2 cut(s)	174, 347
BsaI	GGTCTC	1 cut(s)	2039
BsaJI	CCNNGG	2 cut(s)	284, 2649
BsaWI	WCCGGW	1 cut(s)	456
BsaXI	ACNNNNNCTCC	4 cut(s)	727, 757, 1361, 1391
Bsc4I	CCNNNNNNNGG	2 cut(s)	343, 448
Bse1I	ACTGG	1 cut(s)	2641
Bse3DI	GCAATG	1 cut(s)	1536
BseBI	CCWGG	1 cut(s)	286
BseCI	ATCGAT	1 cut(s)	2097
BseDI	CCNNGG	2 cut(s)	284, 2649
BseGI	GGATG	6 cut(s)	559, 712, 1186, 2171, 2535, 2569
BseLI	CCNNNNNNNGG	2 cut(s)	343, 448
BseMI	GCAATG	1 cut(s)	1536
BseMII	CTCAG	3 cut(s)	112, 438, 2391
BseNI	ACTGG	1 cut(s)	2641
BseRI	GAGGAG	4 cut(s)	1072, 1500, 1549, 1968
BseSI	GKGCMC	1 cut(s)	1882
BseX3I	CGGCCG	1 cut(s)	701
BseXI	GCAGC	2 cut(s)	679, 1037
BseYI	CCCAGC	2 cut(s)	1006, 2369
BsgI	GTGCAG	2 cut(s)	1044, 1275
Bsh1236I	CGCG	2 cut(s)	494, 788
Bsh1285I	CGRYCG	1 cut(s)	704
BshFI	GGCC	3 cut(s)	397, 703, 2068
BshNI	GGYRCC	2 cut(s)	1120, 1928
BshVI	ATCGAT	1 cut(s)	2097
BsiEI	CGRYCG	1 cut(s)	704
BsiHKAI	GWGCWC	3 cut(s)	233, 1882, 2002
BsiSI	CCGG	2 cut(s)	457, 704
BslFI	GGGAC	3 cut(s)	42, 445, 1225
BslI	CCNNNNNNNGG	2 cut(s)	343, 448
BsmAI	GTCTC	1 cut(s)	2039
BsmFI	GGGAC	3 cut(s)	42, 445, 1225
BsmI	GAATGC	3 cut(s)	357, 622, 1502
BsnI	GGCC	3 cut(s)	397, 703, 2068
Bso31I	GGTCTC	1 cut(s)	2039
Bsp1286I	GDGCHC	3 cut(s)	233, 1882, 2002
Bsp1407I	TGTACA	2 cut(s)	717, 932
Bsp143I	GATC	8 cut(s)	8, 264, 1149, 1314, 1966, 2094, 2199, 2308
BspACI	CCGC	5 cut(s)	50, 788, 872, 1028, 1397
BspANI	GGCC	3 cut(s)	397, 703, 2068
BspCNI	CTCAG	3 cut(s)	111, 439, 2390
BspDI	ATCGAT	1 cut(s)	2097
BspFNI	CGCG	2 cut(s)	494, 788
BspHI	TCATGA	2 cut(s)	1516, 2437
BspLI	GGNNCC	6 cut(s)	188, 461, 597, 1122, 1437, 1930
BspPI	GGATC	3 cut(s)	3, 259, 1974
BspT107I	GGYRCC	2 cut(s)	1120, 1928
BspTNI	GGTCTC	1 cut(s)	2039
BsrDI	GCAATG	1 cut(s)	1536
BsrGI	TGTACA	2 cut(s)	717, 932
BsrI	ACTGG	1 cut(s)	2641
BssECI	CCNNGG	2 cut(s)	284, 2649
BssMI	GATC	8 cut(s)	8, 264, 1149, 1314, 1966, 2094, 2199, 2308
BssNI	GRCGYC	2 cut(s)	174, 347
BssSI	CACGAG	1 cut(s)	2261
BssT1I	CCWWGG	1 cut(s)	2649
Bst2BI	CACGAG	1 cut(s)	2261
Bst2UI	CCWGG	1 cut(s)	286
Bst4CI	ACNGT	8 cut(s)	724, 1339, 1444, 1478, 1512, 1688, 1877, 2389
Bst6I	CTCTTC	4 cut(s)	112, 1412, 1525, 2677
BstACI	GRCGYC	2 cut(s)	174, 347
BstAPI	GCANNNNNTGC	1 cut(s)	1928
BstAUI	TGTACA	2 cut(s)	717, 932
BstBAI	YACGTR	1 cut(s)	822
BstC8I	GCNNGC	5 cut(s)	320, 399, 1591, 2221, 2367
BstDEI	CTNAG	4 cut(s)	98, 447, 2377, 2720
BstF5I	GGATG	6 cut(s)	559, 712, 1186, 2171, 2535, 2569
BstFNI	CGCG	2 cut(s)	494, 788
BstHHI	GCGC	2 cut(s)	300, 494
BstKTI	GATC	8 cut(s)	11, 267, 1152, 1317, 1969, 2097, 2202, 2311
BstMAI	GTCTC	1 cut(s)	2039
BstMBI	GATC	8 cut(s)	8, 264, 1149, 1314, 1966, 2094, 2199, 2308
BstMCI	CGRYCG	1 cut(s)	704
BstMWI	GCNNNNNNNGC	2 cut(s)	1142, 1928
BstNI	CCWGG	1 cut(s)	286
BstSCI	CCNGG	1 cut(s)	284
BstSFI	CTRYAG	2 cut(s)	1687, 1873
BstSLI	GKGCMC	1 cut(s)	1882
BstSNI	TACGTA	1 cut(s)	822
BstUI	CGCG	2 cut(s)	494, 788
BstV1I	GCAGC	2 cut(s)	679, 1037
BstV2I	GAAGAC	1 cut(s)	689
BstX2I	RGATCY	1 cut(s)	1966
BstYI	RGATCY	1 cut(s)	1966
BstZI	CGGCCG	1 cut(s)	701
Bsu15I	ATCGAT	1 cut(s)	2097
BsuI	GTATCC	2 cut(s)	1466, 2008
BsuRI	GGCC	3 cut(s)	397, 703, 2068
BsuTUI	ATCGAT	1 cut(s)	2097
BtrI	CACGTC	1 cut(s)	250
BtsCI	GGATG	6 cut(s)	559, 712, 1186, 2171, 2535, 2569
BtsI	GCAGTG	2 cut(s)	1287, 1880
BtsIMutI	CAGTG	8 cut(s)	386, 720, 1287, 1417, 1460, 1880, 1882, 2634
Cac8I	GCNNGC	5 cut(s)	320, 399, 1591, 2221, 2367
CaiI	CAGNNNCTG	1 cut(s)	2549
CciI	TCATGA	2 cut(s)	1516, 2437
CfoI	GCGC	2 cut(s)	300, 494
Cfr13I	GGNCC	1 cut(s)	459
ClaI	ATCGAT	1 cut(s)	2097
CseI	GACGC	1 cut(s)	336
Csp6I	GTAC	5 cut(s)	102, 718, 784, 933, 1684
CviQI	GTAC	5 cut(s)	102, 718, 784, 933, 1684
DdeI	CTNAG	4 cut(s)	98, 447, 2377, 2720
DpnI	GATC	8 cut(s)	10, 266, 1151, 1316, 1968, 2096, 2201, 2310
DpnII	GATC	8 cut(s)	8, 264, 1149, 1314, 1966, 2094, 2199, 2308
DraI	TTTAAA	1 cut(s)	627
EaeI	YGGCCR	1 cut(s)	701
EagI	CGGCCG	1 cut(s)	701
Eam1104I	CTCTTC	4 cut(s)	112, 1412, 1525, 2677
EarI	CTCTTC	4 cut(s)	112, 1412, 1525, 2677
EciI	GGCGGA	1 cut(s)	1412
Ecl136II	GAGCTC	2 cut(s)	231, 2000
EclXI	CGGCCG	1 cut(s)	701
Eco105I	TACGTA	1 cut(s)	822
Eco130I	CCWWGG	1 cut(s)	2649
Eco147I	AGGCCT	1 cut(s)	2068
Eco24I	GRGCYC	2 cut(s)	233, 2002
Eco31I	GGTCTC	1 cut(s)	2039
Eco47I	GGWCC	1 cut(s)	459
Eco52I	CGGCCG	1 cut(s)	701
Eco53kI	GAGCTC	2 cut(s)	231, 2000
Eco57I	CTGAAG	9 cut(s)	193, 621, 1393, 1623, 1833, 2299, 2430, 2606, 2711
EcoICRI	GAGCTC	2 cut(s)	231, 2000
EcoRI	GAATTC	1 cut(s)	469
EcoRII	CCWGG	1 cut(s)	284
EcoT14I	CCWWGG	1 cut(s)	2649
EcoT22I	ATGCAT	4 cut(s)	1147, 1924, 2052, 2104
EcoT38I	GRGCYC	2 cut(s)	233, 2002
ErhI	CCWWGG	1 cut(s)	2649
FalI	AAGNNNNNCTT	2 cut(s)	2226, 2258
FaqI	GGGAC	3 cut(s)	42, 445, 1225
FauNDI	CATATG	2 cut(s)	2104, 2293
FbaI	TGATCA	2 cut(s)	1149, 1314
FblI	GTMKAC	1 cut(s)	1959
Fnu4HI	GCNGC	3 cut(s)	668, 1026, 1029
FokI	GGATG	6 cut(s)	566, 719, 1193, 2178, 2542, 2576
FriOI	GRGCYC	2 cut(s)	233, 2002
Fsp4HI	GCNGC	3 cut(s)	668, 1026, 1029
FspBI	CTAG	6 cut(s)	1707, 1970, 2306, 2573, 2600, 2742
GlaI	GCGC	2 cut(s)	299, 493
GluI	GCNGC	3 cut(s)	668, 1026, 1029
GsaI	CCCAGC	2 cut(s)	1010, 2373
GsuI	CTGGAG	2 cut(s)	217, 2658
HaeIII	GGCC	3 cut(s)	397, 703, 2068
HapII	CCGG	2 cut(s)	457, 704
HgaI	GACGC	1 cut(s)	336
HhaI	GCGC	2 cut(s)	300, 494
Hin1I	GRCGYC	2 cut(s)	174, 347
Hin6I	GCGC	2 cut(s)	298, 492
HinP1I	GCGC	2 cut(s)	298, 492
HindIII	AAGCTT	1 cut(s)	2442
HpaII	CCGG	2 cut(s)	457, 704
HphI	GGTGA	3 cut(s)	649, 1669, 2279
Hpy166II	GTNNAC	5 cut(s)	720, 1096, 1880, 1960, 2482
Hpy188I	TCNGA	7 cut(s)	172, 264, 363, 1813, 2004, 2449, 2586
Hpy188III	TCNNGA	6 cut(s)	253, 268, 883, 1517, 1937, 2438
Hpy8I	GTNNAC	5 cut(s)	720, 1096, 1880, 1960, 2482
Hpy99I	CGWCG	6 cut(s)	146, 176, 179, 254, 352, 499
HpyCH4III	ACNGT	8 cut(s)	724, 1339, 1444, 1478, 1512, 1688, 1877, 2389
HpyCH4IV	ACGT	5 cut(s)	174, 249, 273, 821, 1457
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1142, 1928
HpyF3I	CTNAG	4 cut(s)	98, 447, 2377, 2720
HpySE526I	ACGT	5 cut(s)	174, 249, 273, 821, 1457
Hsp92I	GRCGYC	2 cut(s)	174, 347
HspAI	GCGC	2 cut(s)	298, 492
Ksp22I	TGATCA	2 cut(s)	1149, 1314
Kzo9I	GATC	8 cut(s)	8, 264, 1149, 1314, 1966, 2094, 2199, 2308
LmnI	GCTCC	3 cut(s)	595, 982, 1997
Lsp1109I	GCAGC	2 cut(s)	679, 1037
LweI	GCATC	9 cut(s)	61, 697, 1069, 1909, 2037, 2089, 2156, 2260, 2502
MaeI	CTAG	6 cut(s)	1707, 1970, 2306, 2573, 2600, 2742
MaeII	ACGT	5 cut(s)	174, 249, 273, 821, 1457
MalI	GATC	8 cut(s)	10, 266, 1151, 1316, 1968, 2096, 2201, 2310
MboI	GATC	8 cut(s)	8, 264, 1149, 1314, 1966, 2094, 2199, 2308
MboII	GAAGA	9 cut(s)	99, 689, 1081, 1399, 1542, 1858, 1920, 2034, 2694
MflI	RGATCY	1 cut(s)	1966
MhlI	GDGCHC	3 cut(s)	233, 1882, 2002
MlyI	GAGTC	2 cut(s)	837, 904
MmeI	TCCRAC	3 cut(s)	186, 195, 531
Mph1103I	ATGCAT	4 cut(s)	1147, 1924, 2052, 2104
MroXI	GAANNNNTTC	3 cut(s)	777, 999, 1502
MseI	TTAA	8 cut(s)	236, 306, 486, 626, 1305, 1334, 1778, 1859
MslI	CAYNNNNRTG	2 cut(s)	725, 2055
MspA1I	CMGCKG	2 cut(s)	670, 1028
MspI	CCGG	2 cut(s)	457, 704
MspR9I	CCNGG	1 cut(s)	286
Mva1269I	GAATGC	3 cut(s)	357, 622, 1502
MvaI	CCWGG	1 cut(s)	286
MvnI	CGCG	2 cut(s)	494, 788
MwoI	GCNNNNNNNGC	2 cut(s)	1142, 1928
NdeI	CATATG	2 cut(s)	2104, 2293
NdeII	GATC	8 cut(s)	8, 264, 1149, 1314, 1966, 2094, 2199, 2308
NlaIV	GGNNCC	6 cut(s)	188, 461, 597, 1122, 1437, 1930
NmeAIII	GCCGAG	1 cut(s)	2263
NmuCI	GTSAC	5 cut(s)	1458, 1657, 1937, 2287, 2632
NsiI	ATGCAT	4 cut(s)	1147, 1924, 2052, 2104
OliI	CACNNNNGTG	1 cut(s)	725
PagI	TCATGA	2 cut(s)	1516, 2437
PceI	AGGCCT	1 cut(s)	2068
PcsI	WCGNNNNNNNCGW	3 cut(s)	52, 141, 210
PctI	GAATGC	3 cut(s)	357, 622, 1502
PdmI	GAANNNNTTC	3 cut(s)	777, 999, 1502
PfeI	GAWTC	9 cut(s)	201, 280, 364, 778, 791, 835, 1000, 1259, 1400
PkrI	GCNGC	3 cut(s)	669, 1027, 1030
PleI	GAGTC	2 cut(s)	837, 903
PpsI	GAGTC	2 cut(s)	837, 903
Ppu21I	YACGTR	1 cut(s)	822
PsiI	TTATAA	2 cut(s)	1140, 1854
Psp124BI	GAGCTC	2 cut(s)	233, 2002
Psp6I	CCWGG	1 cut(s)	284
PspFI	CCCAGC	2 cut(s)	1006, 2369
PspGI	CCWGG	1 cut(s)	284
PspN4I	GGNNCC	6 cut(s)	188, 461, 597, 1122, 1437, 1930
PspPI	GGNCC	1 cut(s)	459
PsrI	GAACNNNNNNTAC	1 cut(s)	2722
PstNI	CAGNNNCTG	1 cut(s)	2549
PsuI	RGATCY	1 cut(s)	1966
PvuII	CAGCTG	1 cut(s)	670
RsaI	GTAC	5 cut(s)	103, 719, 785, 934, 1685
RsaNI	GTAC	5 cut(s)	102, 718, 784, 933, 1684
RseI	CAYNNNNRTG	2 cut(s)	725, 2055
SacI	GAGCTC	2 cut(s)	233, 2002
SaqAI	TTAA	8 cut(s)	236, 306, 486, 626, 1305, 1334, 1778, 1859
SatI	GCNGC	3 cut(s)	668, 1026, 1029
Sau3AI	GATC	8 cut(s)	8, 264, 1149, 1314, 1966, 2094, 2199, 2308
Sau96I	GGNCC	1 cut(s)	459
SchI	GAGTC	2 cut(s)	837, 904
ScrFI	CCNGG	1 cut(s)	286
SduI	GDGCHC	3 cut(s)	233, 1882, 2002
SfaNI	GCATC	9 cut(s)	61, 697, 1069, 1909, 2037, 2089, 2156, 2260, 2502
SfcI	CTRYAG	2 cut(s)	1687, 1873
SinI	GGWCC	1 cut(s)	459
SmiMI	CAYNNNNRTG	2 cut(s)	725, 2055
SmlI	CTYRAG	1 cut(s)	2459
SmoI	CTYRAG	1 cut(s)	2459
SnaBI	TACGTA	1 cut(s)	822
SseBI	AGGCCT	1 cut(s)	2068
SsiI	CCGC	5 cut(s)	50, 788, 872, 1028, 1397
SspI	AATATT	1 cut(s)	1281
SspMI	CTAG	6 cut(s)	1707, 1970, 2306, 2573, 2600, 2742
SstI	GAGCTC	2 cut(s)	233, 2002
StuI	AGGCCT	1 cut(s)	2068
StyD4I	CCNGG	1 cut(s)	284
StyI	CCWWGG	1 cut(s)	2649
TaaI	ACNGT	8 cut(s)	724, 1339, 1444, 1478, 1512, 1688, 1877, 2389
TaiI	ACGT	5 cut(s)	177, 252, 276, 824, 1460
TaqI	TCGA	8 cut(s)	7, 181, 227, 252, 898, 1270, 2097, 2701
TatI	WGTACW	2 cut(s)	717, 932
TauI	GCSGC	1 cut(s)	1031
TfiI	GAWTC	9 cut(s)	201, 280, 364, 778, 791, 835, 1000, 1259, 1400
Tru1I	TTAA	8 cut(s)	236, 306, 486, 626, 1305, 1334, 1778, 1859
Tru9I	TTAA	8 cut(s)	236, 306, 486, 626, 1305, 1334, 1778, 1859
TscAI	CASTG	8 cut(s)	386, 727, 1294, 1417, 1467, 1882, 1887, 2641
TseFI	GTSAC	5 cut(s)	1458, 1657, 1937, 2287, 2632
TseI	GCWGC	2 cut(s)	667, 1025
Tsp45I	GTSAC	5 cut(s)	1458, 1657, 1937, 2287, 2632
TspRI	CASTG	8 cut(s)	386, 727, 1294, 1417, 1467, 1882, 1887, 2641
VneI	GTGCAC	1 cut(s)	1878
VpaK11BI	GGWCC	1 cut(s)	459
XapI	RAATTY	5 cut(s)	469, 482, 886, 965, 1613
XmiI	GTMKAC	1 cut(s)	1959
XmnI	GAANNNNTTC	3 cut(s)	777, 999, 1502
XspI	CTAG	6 cut(s)	1707, 1970, 2306, 2573, 2600, 2742
ZraI	GACGTC	1 cut(s)	175
Zsp2I	ATGCAT	4 cut(s)	1147, 1924, 2052, 2104

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.