Rroxscaffold_1G00017250

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016031
Physical Location & Seq
Forward (+)
21405335 .. 21408049
2715 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00017250.1

Sequence Viewer

Length: 2715 bp
ATGAAGCTCACTCGTCCGCATCGTCCCCTCCCAAACCCTCTCATTCACTCTAAACCCAGAAATCTCAGTACCTCTTCCGATTCAACCCCCAAATATGATGACGAAAACGACGACCCCCACTTCATTTCCAACCTATCCGACGTCGTTCGAGGGAAGCAGAGCTGGAGAATCGCCTTCAGCGACCCCTTCATTTCGAGCTCGTTAAAACCCCACCACGTCGAGAAGGTTCTGATCCAGAACGTAAGGAATCCCAGGTTAGCCTTGCGCTTCTTTAATTTTCTGGGCTTGCATAAATCCTTCAACCACTCAACGGCGTCGTTTTGTGTTCTGATTCACGCCCTCGTTCAGTGTAGCCTCTTTTGGCCTGCCACCTCGCTTTTACAGACCCTCTTGCTTCGTGGGTTGAACCCAACTGAGGTTTTCCAGTCCCTTTTGAATTCATATAAGAAATGTAATTGCGCCACGAGTTTGGGTTTTGATTTGTTGGTGCAAAACTATGTGCAAAATAAGAGAGTGTTGGATGGTGTTGTGGTTGTGAGGTTGATGAGGGAGTGTAAAATGGTGCCTGAAGTTAGAACCTTGAATGCTCTTTTAAATGGGTTGGTGAGAATTAGGCATTTCAATATGGTTTTGCAGCTGTTTGATGAGTTGGTCAATGTTGGTCTTCGGCCTGATGCTTATACGTACACTGTGGTGGTTAGGAGTTTGTGTGAGTTGAAGGATTTGCATAAGGCTAATGAAGTGATTCGGTACGCGGAATCAAATGGGTGTGAGTTGAGTGTTGTTACGTATAATGTGTTGATTCATGGACTCTGCAAGAGCCAGAGAGTTTGGGAGGCGGTAGAAATCAAGAATTCATTGAGTCGAAAGGGATTGAAAGCGGATATGGTTACTTATTGTACATTGGTGCTTGGGTTGTGTAAAGTGCAAGAATTTGAGGTTGGTGTGGAGCTAATGAAGGAAATGATTCAGCTGGGGTTTGTTCCAAGTGCAGCAGCTCTTTCGGGTCTTATGGAAGGATTGAGGAGGAAGGGGAAGATTGGAGATGCTTTTGATTTAGTGAACAGAATGGGAGAAGTTGGGGTGGTGCCTAATTTGTTTGCTTATAATGCATTGATCAATTCTTTGTGTAAAGACGGGAAGCTGGATGAGGCAGAATTACTCTTTGACAACATGGGGACTAAGGGTTTGTTTCCCAATGATGTTACATATTCTATTTTGATTGATTCATTCTGTCGAAGGGGAATATTGGACACTGCACATTGTTATTTTAATAAAATGATCAATGCTAGGATTAGATTAACTGTATATCCCTACAATTCTTTGATAAGTGGGGAGTGCAAGTTTGGAAAGTTAAGTGTGGCGGAATCTCTCTTCAGTGAGATGATGAGTAGAAGTGTGGAACCAACTGTTGTTACTTATACGTCACTGATAAGTGGATACTGTAAAGGAGGAGAACTGCAGAAGGCATTCATACTGTATCATGAAATGATGGGAAGAGGCATTGCACCAAATACTTATACCTTCTCTGCAATTATTTCAGGTCTTTGTCGTGCAAGCATGATGGCTGAAGCAAGTAAATTCTTTGATGAAATGGTGAAAAGGGGCATTATGCCAAACGAGGTGACATACAATCTTTTGATTGATGGGTACTGTAGGGAAGGTAACATTACTAGAGCTTTTAAATTGCTTGATGAAATGCTCAAAAAGGGGCTTCTACCGGACACATATACATATAGACCCTTAATAAGTGGTCTTTGTTCCACAAGTGGAGTGTCTGAAGCCAAAAAGTTTGTAGATGACCTTCACAAGAAGAATTGTAAGTTAAATGAGATGTGCTACAGTGCACTGCTGTATGGTTATTGCAAGGAAGGAAGATTATATGATGCATTAGGTGCCTGTCGTGACATGAGTGAAAGAGGAGTAGACATGGATCTAGTGTGTTATGCCATACTTATCTGTGGAGCTCTGAAACAACAGGATACCCGAAGATTATTTGGTCTCGTAAATGAGATGCATAGTCAAGGATTGAGGCCTGATAATGTAATCTATACAAGTATGATTGATGCATACGGGAAATCAGGAAAACTTGATAAGGCATTTGGACTTTGGGATATAATGGTTGGTGAAGGATGCTCTCCTAATGTTGTAACATACACAGCCTTGATCTATGGATTATGTAAGGCAGGCTGTATTGATAAAGCCGAGCTTCTTTGCAAGGATATGCTCGTCGGTGATGCCCTTCCCAATCATGTCACATATGGTTGTTTCCTAGATCACTTCAGCAAAGAAGGTAGCATGGAGAAAGCTGTGCAACTGCACAACACCATGCTTGCTGGGTTCTCAGCCAATACTGTCACATACAATATACTTATTAGGGGTTTCTGCAAAATGGGTAATTTTCATGAAGCTTCAGAACTCTTGCTTGAGATGACAAACAATGGTGTTTACCCTGATTGTATCACCTATTCAACATTTATCTTTGAGTATTGTAGGATGGGCAATTTGCCAGAAGCTGTTAGGTTGTGGGATGTTATGCTAGATAGGGGTCTGAAGCCTGATATTCTAGCGTATAACTTTTTGATATATGGTTGCTGTGTCACTGGAGAACTAACCAAGGCTTTTGAGTTGCGTGATGACATGATGAGAAGAGGGCTGAAGCCAAATCGAGTTACATATAACACACTAAGCCGTGGAACTTGCCTTACTAGGTAA

Protein Analysis

904

Amino Acids

101.62

Weight (kDa)

8.39

Isoelectric Point (pI)

26.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 189 - 237 6.3e-11 PPR repeat family
PPR_3 PF13812 213 - 269 3.2e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 234 - 273 4.9e-07 PPR repeat family
PPR_1 PF12854 255 - 283 5.3e-09 PPR repeat
PPR_2 PF13041 260 - 308 1.7e-12 PPR repeat family
PPR_long PF17177 313 - 428 1.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 337 - 378 3.2e-08 PPR repeat family
PPR_1 PF12854 361 - 392 1.2e-11 PPR repeat
PPR_2 PF13041 364 - 413 2e-17 PPR repeat family
PPR PF01535 368 - 397 1.9e-07 PPR repeat
PPR_1 PF12854 395 - 427 9.1e-09 PPR repeat
PPR_3 PF13812 425 - 479 2.9e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 438 - 483 1.1e-14 PPR repeat family
PPR PF01535 439 - 466 5.5e-06 PPR repeat
PPR_3 PF13812 457 - 514 1.8e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 466 - 497 1.4e-10 PPR repeat
PPR_2 PF13041 469 - 518 1.2e-18 PPR repeat family
PPR PF01535 472 - 502 6.8e-09 PPR repeat
PPR_long PF17177 493 - 626 2e-10 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 500 - 533 3.6e-11 PPR repeat
PPR PF01535 507 - 537 8.7e-07 PPR repeat
PPR_2 PF13041 511 - 553 6.7e-15 PPR repeat family
TPR_24 PF23276 525 - 633 1.2e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 529 - 584 3.9e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 535 - 567 1e-14 PPR repeat
PPR_2 PF13041 539 - 587 1.8e-19 PPR repeat family
PPR PF01535 542 - 572 9.1e-10 PPR repeat
PPR_2 PF13041 582 - 623 4.8e-06 PPR repeat family
PPR_2 PF13041 610 - 655 3e-07 PPR repeat family
PPR_3 PF13812 668 - 723 1.5e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 676 - 708 3e-08 PPR repeat
PPR_2 PF13041 679 - 728 2.4e-17 PPR repeat family
PPR PF01535 682 - 712 2.3e-08 PPR repeat
PPR_1 PF12854 711 - 742 4.4e-11 PPR repeat
PPR_2 PF13041 714 - 756 8.7e-09 PPR repeat family
PPR_3 PF13812 776 - 827 3.1e-06 Pentatricopeptide repeat domain
PPR_long PF17177 779 - 896 5.7e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 780 - 811 3.4e-11 PPR repeat
PPR_2 PF13041 784 - 832 3.6e-15 PPR repeat family
PPR PF01535 786 - 816 8.8e-09 PPR repeat
PPR_1 PF12854 814 - 846 4.4e-09 PPR repeat
PPR_2 PF13041 821 - 866 4.8e-11 PPR repeat family
PPR_3 PF13812 843 - 896 5.3e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 849 - 881 1.6e-06 PPR repeat
PPR_2 PF13041 853 - 901 3.3e-14 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59900
fragaria_vesca FvH4_3g35360
malus_domestica MD11G1144200.v1.1
prunus_persica Prupe.6G082100_v2.0.a1
pyrus_communis pycom11g09360
rosa_chinensis RchiOBHm_Chr5g0063471
rosa_laevigata RLG00000035637
rosa_multiflora Rmu_sc0016394.1_g000003
rosa_roxburghii Rroxscaffold_1G00017250
rosa_rugosa Rorug05G0357500
rosa_samantha Rh5AG416600 Rh5BG431800 Rh5CG455100 Rh5DG445300
rosa_wichuraiana Rw5G039140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1107
AatII GACGTC 1 cut(s) 144
AccB1I GGYRCC 3 cut(s) 562, 1087, 1895
AccI GTMKAC 1 cut(s) 1926
AccII CGCG 1 cut(s) 755
AciI CCGC 5 cut(s) 17, 755, 839, 881, 1364
AclWI GGATC 2 cut(s) 226, 1941
AcsI RAATTY 4 cut(s) 436, 853, 932, 1580
AcuI CTGAAG 9 cut(s) 160, 588, 1360, 1590, 1800, 2266, 2397, 2573, 2678
AcyI GRCGYC 2 cut(s) 141, 314
AfaI GTAC 5 cut(s) 70, 686, 752, 901, 1652
AfiI CCNNNNNNNGG 2 cut(s) 310, 415
AgsI TTSAA 9 cut(s) 84, 301, 406, 436, 583, 622, 718, 877, 2472
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 1 cut(s) 251
AjuI GAANNNNNNNTTGG 2 cut(s) 924, 956
AleI CACNNNNGTG 1 cut(s) 692
Alw21I GWGCWC 3 cut(s) 200, 1849, 1969
Alw26I GTCTC 1 cut(s) 2006
Alw44I GTGCAC 1 cut(s) 1845
AlwI GGATC 2 cut(s) 226, 1941
AlwNI CAGNNNCTG 1 cut(s) 2516
AoxI GGCC 3 cut(s) 362, 668, 2033
ApaLI GTGCAC 1 cut(s) 1845
ApeKI GCWGC 3 cut(s) 634, 992, 995
ApoI RAATTY 4 cut(s) 436, 853, 932, 1580
ArsI GACNNNNNNTTYG 2 cut(s) 1171, 1203
Asp700I GAANNNNTTC 3 cut(s) 744, 966, 1469
AspLEI GCGC 2 cut(s) 267, 461
AsuHPI GGTGA 6 cut(s) 616, 1609, 1636, 2138, 2246, 2455
BaeGI GKGCMC 1 cut(s) 1849
BanI GGYRCC 3 cut(s) 562, 1087, 1895
BanII GRGCYC 2 cut(s) 200, 1969
BarI GAAGNNNNNNTAC 2 cut(s) 1788, 1820
BauI CACGAG 1 cut(s) 463
BbsI GAAGAC 1 cut(s) 656
Bbv12I GWGCWC 3 cut(s) 200, 1849, 1969
BbvI GCAGC 3 cut(s) 646, 1004, 1007
BccI CCATC 5 cut(s) 515, 1486, 1558, 1640, 2491
BceAI ACGGC 2 cut(s) 327, 2676
BcgI CGANNNNNNTGC 2 cut(s) 984, 1018
BciT130I CCWGG 1 cut(s) 253
BciVI GTATCC 2 cut(s) 1433, 1975
BclI TGATCA 2 cut(s) 1116, 1281
BcoDI GTCTC 1 cut(s) 2006
BfaI CTAG 7 cut(s) 1290, 1674, 1937, 2273, 2540, 2567, 2709
BfmI CTRYAG 3 cut(s) 1460, 1654, 1840
BfuI GTATCC 2 cut(s) 1433, 1975
BisI GCNGC 3 cut(s) 635, 993, 996
BlsI GCNGC 3 cut(s) 636, 994, 997
Bme1390I CCNGG 1 cut(s) 253
BmgBI CACGTC 1 cut(s) 217
BmiI GGNNCC 4 cut(s) 564, 1089, 1404, 1897
BmrFI CCNGG 1 cut(s) 253
BmsI GCATC 8 cut(s) 28, 664, 1036, 1876, 2004, 2056, 2123, 2227
BpiI GAAGAC 1 cut(s) 656
BpmI CTGGAG 2 cut(s) 184, 2625
BpuEI CTTGAG 1 cut(s) 2447
BsaAI YACGTR 2 cut(s) 684, 789
BsaHI GRCGYC 2 cut(s) 141, 314
BsaI GGTCTC 1 cut(s) 2006
BsaJI CCNNGG 3 cut(s) 251, 2616, 2692
BsaWI WCCGGW 1 cut(s) 1720
BsaXI ACNNNNNCTCC 4 cut(s) 694, 724, 1328, 1358
Bsc4I CCNNNNNNNGG 2 cut(s) 310, 415
Bse1I ACTGG 2 cut(s) 424, 2608
Bse3DI GCAATG 1 cut(s) 1503
BseBI CCWGG 1 cut(s) 253
BseDI CCNNGG 3 cut(s) 251, 2616, 2692
BseGI GGATG 5 cut(s) 526, 1153, 2138, 2502, 2536
BseLI CCNNNNNNNGG 2 cut(s) 310, 415
BseMI GCAATG 1 cut(s) 1503
BseMII CTCAG 3 cut(s) 79, 405, 2358
BseNI ACTGG 2 cut(s) 424, 2608
BseRI GAGGAG 3 cut(s) 1039, 1467, 1935
BseSI GKGCMC 1 cut(s) 1849
BseXI GCAGC 3 cut(s) 646, 1004, 1007
BseYI CCCAGC 2 cut(s) 973, 2336
BsgI GTGCAG 3 cut(s) 1011, 1242, 2303
Bsh1236I CGCG 1 cut(s) 755
BshFI GGCC 3 cut(s) 364, 670, 2035
BshNI GGYRCC 3 cut(s) 562, 1087, 1895
BsiHKAI GWGCWC 3 cut(s) 200, 1849, 1969
BsiSI CCGG 1 cut(s) 1721
BslFI GGGAC 3 cut(s) 9, 412, 1192
BslI CCNNNNNNNGG 2 cut(s) 310, 415
BsmAI GTCTC 1 cut(s) 2006
BsmFI GGGAC 3 cut(s) 9, 412, 1192
BsmI GAATGC 2 cut(s) 589, 1469
BsnI GGCC 3 cut(s) 364, 670, 2035
Bso31I GGTCTC 1 cut(s) 2006
Bsp1286I GDGCHC 3 cut(s) 200, 1849, 1969
Bsp1407I TGTACA 1 cut(s) 899
Bsp143I GATC 6 cut(s) 231, 1116, 1281, 1933, 2166, 2275
BspACI CCGC 5 cut(s) 17, 755, 839, 881, 1364
BspANI GGCC 3 cut(s) 364, 670, 2035
BspCNI CTCAG 3 cut(s) 78, 406, 2357
BspFNI CGCG 1 cut(s) 755
BspHI TCATGA 2 cut(s) 1483, 2404
BspLI GGNNCC 4 cut(s) 564, 1089, 1404, 1897
BspMAI CTGCAG 1 cut(s) 1464
BspPI GGATC 2 cut(s) 226, 1941
BspT107I GGYRCC 3 cut(s) 562, 1087, 1895
BspTNI GGTCTC 1 cut(s) 2006
BsrDI GCAATG 1 cut(s) 1503
BsrGI TGTACA 1 cut(s) 899
BsrI ACTGG 2 cut(s) 424, 2608
BssECI CCNNGG 3 cut(s) 251, 2616, 2692
BssMI GATC 6 cut(s) 231, 1116, 1281, 1933, 2166, 2275
BssNI GRCGYC 2 cut(s) 141, 314
BssSI CACGAG 1 cut(s) 463
BssT1I CCWWGG 1 cut(s) 2616
Bst2BI CACGAG 1 cut(s) 463
Bst2UI CCWGG 1 cut(s) 253
Bst4CI ACNGT 8 cut(s) 691, 1306, 1411, 1445, 1479, 1655, 1844, 2356
Bst6I CTCTTC 4 cut(s) 79, 1379, 1492, 2644
BstACI GRCGYC 2 cut(s) 141, 314
BstAPI GCANNNNNTGC 1 cut(s) 1895
BstAUI TGTACA 1 cut(s) 899
BstBAI YACGTR 2 cut(s) 684, 789
BstC8I GCNNGC 5 cut(s) 287, 366, 1558, 2188, 2334
BstDEI CTNAG 5 cut(s) 65, 414, 1182, 2344, 2687
BstDSI CCRYGG 1 cut(s) 2692
BstF5I GGATG 5 cut(s) 526, 1153, 2138, 2502, 2536
BstFNI CGCG 1 cut(s) 755
BstHHI GCGC 2 cut(s) 267, 461
BstKTI GATC 6 cut(s) 234, 1119, 1284, 1936, 2169, 2278
BstMAI GTCTC 1 cut(s) 2006
BstMBI GATC 6 cut(s) 231, 1116, 1281, 1933, 2166, 2275
BstMWI GCNNNNNNNGC 2 cut(s) 1109, 1895
BstNI CCWGG 1 cut(s) 253
BstSCI CCNGG 1 cut(s) 251
BstSFI CTRYAG 3 cut(s) 1460, 1654, 1840
BstSLI GKGCMC 1 cut(s) 1849
BstSNI TACGTA 2 cut(s) 684, 789
BstUI CGCG 1 cut(s) 755
BstV1I GCAGC 3 cut(s) 646, 1004, 1007
BstV2I GAAGAC 1 cut(s) 656
BstX2I RGATCY 1 cut(s) 1933
BstXI CCANNNNNNTGG 1 cut(s) 469
BstYI RGATCY 1 cut(s) 1933
BsuI GTATCC 2 cut(s) 1433, 1975
BsuRI GGCC 3 cut(s) 364, 670, 2035
BtgI CCRYGG 1 cut(s) 2692
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 5 cut(s) 526, 1153, 2138, 2502, 2536
BtsI GCAGTG 2 cut(s) 1254, 1847
BtsIMutI CAGTG 8 cut(s) 353, 687, 1254, 1384, 1427, 1847, 1849, 2601
Cac8I GCNNGC 5 cut(s) 287, 366, 1558, 2188, 2334
CaiI CAGNNNCTG 1 cut(s) 2516
CciI TCATGA 2 cut(s) 1483, 2404
CfoI GCGC 2 cut(s) 267, 461
CseI GACGC 1 cut(s) 303
Csp6I GTAC 5 cut(s) 69, 685, 751, 900, 1651
CviQI GTAC 5 cut(s) 69, 685, 751, 900, 1651
DdeI CTNAG 5 cut(s) 65, 414, 1182, 2344, 2687
DpnI GATC 6 cut(s) 233, 1118, 1283, 1935, 2168, 2277
DpnII GATC 6 cut(s) 231, 1116, 1281, 1933, 2166, 2275
DraI TTTAAA 2 cut(s) 594, 1684
Eam1104I CTCTTC 4 cut(s) 79, 1379, 1492, 2644
EarI CTCTTC 4 cut(s) 79, 1379, 1492, 2644
EciI GGCGGA 1 cut(s) 1379
Ecl136II GAGCTC 2 cut(s) 198, 1967
Eco105I TACGTA 2 cut(s) 684, 789
Eco130I CCWWGG 1 cut(s) 2616
Eco147I AGGCCT 1 cut(s) 2035
Eco24I GRGCYC 2 cut(s) 200, 1969
Eco31I GGTCTC 1 cut(s) 2006
Eco53kI GAGCTC 2 cut(s) 198, 1967
Eco57I CTGAAG 9 cut(s) 160, 588, 1360, 1590, 1800, 2266, 2397, 2573, 2678
EcoICRI GAGCTC 2 cut(s) 198, 1967
EcoRI GAATTC 2 cut(s) 436, 853
EcoRII CCWGG 1 cut(s) 251
EcoT14I CCWWGG 1 cut(s) 2616
EcoT22I ATGCAT 4 cut(s) 1114, 1891, 2019, 2071
EcoT38I GRGCYC 2 cut(s) 200, 1969
ErhI CCWWGG 1 cut(s) 2616
FalI AAGNNNNNCTT 2 cut(s) 2193, 2225
FaqI GGGAC 3 cut(s) 9, 412, 1192
FauNDI CATATG 1 cut(s) 2260
FbaI TGATCA 2 cut(s) 1116, 1281
FblI GTMKAC 1 cut(s) 1926
Fnu4HI GCNGC 3 cut(s) 635, 993, 996
FokI GGATG 5 cut(s) 533, 1160, 2145, 2509, 2543
FriOI GRGCYC 2 cut(s) 200, 1969
Fsp4HI GCNGC 3 cut(s) 635, 993, 996
FspBI CTAG 7 cut(s) 1290, 1674, 1937, 2273, 2540, 2567, 2709
GlaI GCGC 2 cut(s) 266, 460
GluI GCNGC 3 cut(s) 635, 993, 996
GsaI CCCAGC 2 cut(s) 977, 2340
GsuI CTGGAG 2 cut(s) 184, 2625
HaeIII GGCC 3 cut(s) 364, 670, 2035
HapII CCGG 1 cut(s) 1721
HgaI GACGC 1 cut(s) 303
HhaI GCGC 2 cut(s) 267, 461
Hin1I GRCGYC 2 cut(s) 141, 314
Hin6I GCGC 2 cut(s) 265, 459
HinP1I GCGC 2 cut(s) 265, 459
HindIII AAGCTT 1 cut(s) 2409
HpaII CCGG 1 cut(s) 1721
HphI GGTGA 6 cut(s) 616, 1609, 1636, 2138, 2246, 2455
Hpy166II GTNNAC 5 cut(s) 687, 1063, 1847, 1927, 2449
Hpy188I TCNGA 8 cut(s) 79, 139, 231, 330, 1780, 1971, 2416, 2553
Hpy188III TCNNGA 7 cut(s) 220, 235, 850, 1484, 1904, 2082, 2405
Hpy8I GTNNAC 5 cut(s) 687, 1063, 1847, 1927, 2449
Hpy99I CGWCG 6 cut(s) 113, 143, 146, 221, 319, 2234
HpyCH4III ACNGT 8 cut(s) 691, 1306, 1411, 1445, 1479, 1655, 1844, 2356
HpyCH4IV ACGT 6 cut(s) 141, 216, 240, 683, 788, 1424
HpyF10VI GCNNNNNNNGC 2 cut(s) 1109, 1895
HpyF3I CTNAG 5 cut(s) 65, 414, 1182, 2344, 2687
HpySE526I ACGT 6 cut(s) 141, 216, 240, 683, 788, 1424
Hsp92I GRCGYC 2 cut(s) 141, 314
HspAI GCGC 2 cut(s) 265, 459
Ksp22I TGATCA 2 cut(s) 1116, 1281
Kzo9I GATC 6 cut(s) 231, 1116, 1281, 1933, 2166, 2275
LmnI GCTCC 2 cut(s) 949, 1964
Lsp1109I GCAGC 3 cut(s) 646, 1004, 1007
LweI GCATC 8 cut(s) 28, 664, 1036, 1876, 2004, 2056, 2123, 2227
MaeI CTAG 7 cut(s) 1290, 1674, 1937, 2273, 2540, 2567, 2709
MaeII ACGT 6 cut(s) 141, 216, 240, 683, 788, 1424
MalI GATC 6 cut(s) 233, 1118, 1283, 1935, 2168, 2277
MboI GATC 6 cut(s) 231, 1116, 1281, 1933, 2166, 2275
MboII GAAGA 9 cut(s) 66, 656, 1048, 1366, 1509, 1825, 1887, 2001, 2661
MflI RGATCY 1 cut(s) 1933
MhlI GDGCHC 3 cut(s) 200, 1849, 1969
MlyI GAGTC 2 cut(s) 804, 871
MmeI TCCRAC 3 cut(s) 153, 162, 498
Mph1103I ATGCAT 4 cut(s) 1114, 1891, 2019, 2071
MroXI GAANNNNTTC 3 cut(s) 744, 966, 1469
MseI TTAA 9 cut(s) 203, 273, 593, 1272, 1301, 1355, 1683, 1745, 1826
MslI CAYNNNNRTG 1 cut(s) 692
MspA1I CMGCKG 2 cut(s) 637, 973
MspI CCGG 1 cut(s) 1721
MspR9I CCNGG 1 cut(s) 253
Mva1269I GAATGC 2 cut(s) 589, 1469
MvaI CCWGG 1 cut(s) 253
MvnI CGCG 1 cut(s) 755
MwoI GCNNNNNNNGC 2 cut(s) 1109, 1895
NdeI CATATG 1 cut(s) 2260
NdeII GATC 6 cut(s) 231, 1116, 1281, 1933, 2166, 2275
NlaIV GGNNCC 4 cut(s) 564, 1089, 1404, 1897
NmeAIII GCCGAG 1 cut(s) 2230
NmuCI GTSAC 6 cut(s) 1425, 1624, 1904, 2254, 2356, 2599
NsiI ATGCAT 4 cut(s) 1114, 1891, 2019, 2071
OliI CACNNNNGTG 1 cut(s) 692
PagI TCATGA 2 cut(s) 1483, 2404
PceI AGGCCT 1 cut(s) 2035
PcsI WCGNNNNNNNCGW 3 cut(s) 19, 108, 177
PctI GAATGC 2 cut(s) 589, 1469
PdmI GAANNNNTTC 3 cut(s) 744, 966, 1469
PkrI GCNGC 3 cut(s) 636, 994, 997
PleI GAGTC 2 cut(s) 804, 870
PpsI GAGTC 2 cut(s) 804, 870
Ppu21I YACGTR 2 cut(s) 684, 789
PsiI TTATAA 1 cut(s) 1107
Psp124BI GAGCTC 2 cut(s) 200, 1969
Psp6I CCWGG 1 cut(s) 251
PspFI CCCAGC 2 cut(s) 973, 2336
PspGI CCWGG 1 cut(s) 251
PspN4I GGNNCC 4 cut(s) 564, 1089, 1404, 1897
PsrI GAACNNNNNNTAC 1 cut(s) 2689
PstI CTGCAG 1 cut(s) 1464
PstNI CAGNNNCTG 1 cut(s) 2516
PsuI RGATCY 1 cut(s) 1933
PvuII CAGCTG 2 cut(s) 637, 973
RsaI GTAC 5 cut(s) 70, 686, 752, 901, 1652
RsaNI GTAC 5 cut(s) 69, 685, 751, 900, 1651
RseI CAYNNNNRTG 1 cut(s) 692
SacI GAGCTC 2 cut(s) 200, 1969
SaqAI TTAA 9 cut(s) 203, 273, 593, 1272, 1301, 1355, 1683, 1745, 1826
SatI GCNGC 3 cut(s) 635, 993, 996
Sau3AI GATC 6 cut(s) 231, 1116, 1281, 1933, 2166, 2275
SchI GAGTC 2 cut(s) 804, 871
ScrFI CCNGG 1 cut(s) 253
SduI GDGCHC 3 cut(s) 200, 1849, 1969
SfaNI GCATC 8 cut(s) 28, 664, 1036, 1876, 2004, 2056, 2123, 2227
SfcI CTRYAG 3 cut(s) 1460, 1654, 1840
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 1 cut(s) 2426
SmoI CTYRAG 1 cut(s) 2426
SnaBI TACGTA 2 cut(s) 684, 789
SseBI AGGCCT 1 cut(s) 2035
SsiI CCGC 5 cut(s) 17, 755, 839, 881, 1364
SspI AATATT 1 cut(s) 1248
SspMI CTAG 7 cut(s) 1290, 1674, 1937, 2273, 2540, 2567, 2709
SstI GAGCTC 2 cut(s) 200, 1969
StuI AGGCCT 1 cut(s) 2035
StyD4I CCNGG 1 cut(s) 251
StyI CCWWGG 1 cut(s) 2616
TaaI ACNGT 8 cut(s) 691, 1306, 1411, 1445, 1479, 1655, 1844, 2356
TaiI ACGT 6 cut(s) 144, 219, 243, 686, 791, 1427
TaqI TCGA 6 cut(s) 148, 194, 219, 865, 1237, 2668
TatI WGTACW 1 cut(s) 899
Tru1I TTAA 9 cut(s) 203, 273, 593, 1272, 1301, 1355, 1683, 1745, 1826
Tru9I TTAA 9 cut(s) 203, 273, 593, 1272, 1301, 1355, 1683, 1745, 1826
TscAI CASTG 8 cut(s) 353, 694, 1261, 1384, 1434, 1849, 1854, 2608
TseFI GTSAC 6 cut(s) 1425, 1624, 1904, 2254, 2356, 2599
TseI GCWGC 3 cut(s) 634, 992, 995
Tsp45I GTSAC 6 cut(s) 1425, 1624, 1904, 2254, 2356, 2599
TspRI CASTG 8 cut(s) 353, 694, 1261, 1384, 1434, 1849, 1854, 2608
VneI GTGCAC 1 cut(s) 1845
XapI RAATTY 4 cut(s) 436, 853, 932, 1580
XmiI GTMKAC 1 cut(s) 1926
XmnI GAANNNNTTC 3 cut(s) 744, 966, 1469
XspI CTAG 7 cut(s) 1290, 1674, 1937, 2273, 2540, 2567, 2709
ZraI GACGTC 1 cut(s) 142
Zsp2I ATGCAT 4 cut(s) 1114, 1891, 2019, 2071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.