RchiOBHm_Chr6g0244381

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0017321
Physical Location & Seq
Reverse (-)
481005 .. 484187
3183 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21902

Sequence Viewer

Length: 570 bp
ATGGACCATGGTAGCTTAGGAATGGAATGGAATTACTTGATGATGAATCGGGCTAGGTTGCACTTGCAGCCATGGACTGCCAGTGTCTTGATGTTACAAAGGACCTCATCAAGCTTGTATGAAAGGCATTTCCAGAGAGTAAAAGAGTTGGTTAGCAAGCTAGAGGCTATGTTGTTTGAGGCAAAGGGATCCTGGGCAGAGGCAGAGAAGGCTGATCTAATATGTGAATTTCAATCCATCCTTCTTTTGCGGGTAATACATAACAGAAGGGTAGATATGGCAAAGGCTCAAGGCGATACTTCAGGGGCCATTGAATGGTTGAATAAATATCTTGAAATATTTATGGCCGATAATGATGCTTGGAGAGAGCTGGCAGAAATCTATGTCTCATTGCAAATAATTTGTGCAAGTTTTCACTCTTCACACCTCAAAATGCAAGGTTGGGAGCGTGGTGCAGGAGCAACACTGGCTTGTGGAACTGGTGCTTGTGTTGTAGTTGTAGCAGCAGTTCTTGAGGGTCGTGCTGGGCGGATAAGTTTTGGACTTTTGGGCTATGTGTTAGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_19

Protein Analysis

189

Amino Acids

21.28

Weight (kDa)

6.08

Isoelectric Point (pI)

33.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_EMC2 PF22890 52 - 134 5e-27 EMC2 TPR-like repeat domain
DAP_epimerase PF01678 139 - 174 1.1e-07 Diaminopimelate epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017321)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g03340
rosa_chinensis RchiOBHm_Chr5g0051541 RchiOBHm_Chr6g0244381 RchiOBHm_Chr7g0229041
rosa_laevigata RLG00000003551 RLG00000007409
rosa_multiflora Rmu_co8186706.1_g000001 Rmu_co8442279.1_g000001
rosa_roxburghii Rroxscaffold_7G00158180
rosa_rugosa Rorug05G0508200
rosa_samantha Rh6AG023300 Rh7DG291700
rosa_wichuraiana Rw2G024010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 315
AciI CCGC 2 cut(s) 250, 529
AclWI GGATC 2 cut(s) 183, 196
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 1 cut(s) 227
AcuI CTGAAG 1 cut(s) 285
AfiI CCNNNNNNNGG 1 cut(s) 315
AgsI TTSAA 4 cut(s) 233, 314, 322, 335
AjnI CCWGG 1 cut(s) 191
AluBI AGCT 4 cut(s) 15, 114, 160, 370
AluI AGCT 4 cut(s) 15, 114, 160, 370
Alw26I GTCTC 1 cut(s) 391
AlwI GGATC 2 cut(s) 183, 196
AoxI GGCC 2 cut(s) 306, 345
ApeKI GCWGC 2 cut(s) 67, 503
ApoI RAATTY 1 cut(s) 227
AspS9I GGNCC 3 cut(s) 4, 102, 306
AvaII GGWCC 2 cut(s) 4, 102
BamHI GGATCC 1 cut(s) 188
BbvI GCAGC 2 cut(s) 79, 515
BccI CCATC 1 cut(s) 245
BcgI CGANNNNNNTGC 2 cut(s) 338, 372
BciT130I CCWGG 1 cut(s) 193
BcoDI GTCTC 1 cut(s) 391
BfaI CTAG 2 cut(s) 54, 161
BisI GCNGC 2 cut(s) 68, 504
BlsI GCNGC 2 cut(s) 69, 505
Bme1390I CCNGG 1 cut(s) 193
Bme18I GGWCC 2 cut(s) 4, 102
BmgT120I GGNCC 3 cut(s) 4, 102, 306
BmiI GGNNCC 2 cut(s) 190, 307
BmrFI CCNGG 1 cut(s) 193
BmsI GCATC 1 cut(s) 346
Bpu10I CCTNAGC 1 cut(s) 16
BpuEI CTTGAG 2 cut(s) 273, 533
BsaJI CCNNGG 3 cut(s) 7, 71, 192
Bsc4I CCNNNNNNNGG 1 cut(s) 315
Bse1I ACTGG 3 cut(s) 81, 471, 484
Bse3DI GCAATG 1 cut(s) 389
BseBI CCWGG 1 cut(s) 193
BseDI CCNNGG 3 cut(s) 7, 71, 192
BseGI GGATG 1 cut(s) 237
BseLI CCNNNNNNNGG 1 cut(s) 315
BseMI GCAATG 1 cut(s) 389
BseNI ACTGG 3 cut(s) 81, 471, 484
BseXI GCAGC 2 cut(s) 79, 515
BseYI CCCAGC 1 cut(s) 524
BsgI GTGCAG 1 cut(s) 474
BshFI GGCC 2 cut(s) 308, 347
BslI CCNNNNNNNGG 1 cut(s) 315
BsmAI GTCTC 1 cut(s) 391
BsnI GGCC 2 cut(s) 308, 347
Bsp143I GATC 2 cut(s) 188, 214
Bsp19I CCATGG 2 cut(s) 7, 71
BspACI CCGC 2 cut(s) 250, 529
BspANI GGCC 2 cut(s) 308, 347
BspLI GGNNCC 2 cut(s) 190, 307
BspPI GGATC 2 cut(s) 183, 196
BsrDI GCAATG 1 cut(s) 389
BsrI ACTGG 3 cut(s) 81, 471, 484
BssECI CCNNGG 3 cut(s) 7, 71, 192
BssMI GATC 2 cut(s) 188, 214
BssT1I CCWWGG 2 cut(s) 7, 71
Bst2UI CCWGG 1 cut(s) 193
Bst6I CTCTTC 1 cut(s) 424
BstC8I GCNNGC 2 cut(s) 158, 372
BstDEI CTNAG 1 cut(s) 16
BstDSI CCRYGG 2 cut(s) 7, 71
BstF5I GGATG 1 cut(s) 237
BstKTI GATC 2 cut(s) 191, 217
BstMAI GTCTC 1 cut(s) 391
BstMBI GATC 2 cut(s) 188, 214
BstMWI GCNNNNNNNGC 3 cut(s) 67, 209, 467
BstNI CCWGG 1 cut(s) 193
BstSCI CCNGG 1 cut(s) 191
BstV1I GCAGC 2 cut(s) 79, 515
BstX2I RGATCY 1 cut(s) 188
BstYI RGATCY 1 cut(s) 188
BsuRI GGCC 2 cut(s) 308, 347
BtgI CCRYGG 2 cut(s) 7, 71
BtsCI GGATG 1 cut(s) 237
BtsIMutI CAGTG 2 cut(s) 88, 464
Cac8I GCNNGC 2 cut(s) 158, 372
Cfr13I GGNCC 3 cut(s) 4, 102, 306
CviAII CATG 2 cut(s) 8, 72
DdeI CTNAG 1 cut(s) 16
DpnI GATC 2 cut(s) 190, 216
DpnII GATC 2 cut(s) 188, 214
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 1 cut(s) 424
EarI CTCTTC 1 cut(s) 424
EciI GGCGGA 1 cut(s) 544
Eco130I CCWWGG 2 cut(s) 7, 71
Eco47I GGWCC 2 cut(s) 4, 102
Eco57I CTGAAG 1 cut(s) 285
EcoO109I RGGNCCY 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 191
EcoT14I CCWWGG 2 cut(s) 7, 71
ErhI CCWWGG 2 cut(s) 7, 71
FaeI CATG 2 cut(s) 11, 75
FatI CATG 2 cut(s) 7, 71
FauI CCCGC 1 cut(s) 243
Fnu4HI GCNGC 2 cut(s) 68, 504
FokI GGATG 1 cut(s) 224
Fsp4HI GCNGC 2 cut(s) 68, 504
FspBI CTAG 2 cut(s) 54, 161
GluI GCNGC 2 cut(s) 68, 504
GsaI CCCAGC 1 cut(s) 528
HaeIII GGCC 2 cut(s) 308, 347
Hin1II CATG 2 cut(s) 11, 75
HindIII AAGCTT 1 cut(s) 112
HinfI GANTC 1 cut(s) 46
Hpy188III TCNNGA 4 cut(s) 88, 133, 332, 512
HpyAV CCTTC 3 cut(s) 202, 251, 261
HpyCH4V TGCA 6 cut(s) 61, 67, 394, 407, 436, 455
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 209, 467
HpyF3I CTNAG 1 cut(s) 16
Hsp92II CATG 2 cut(s) 11, 75
Kzo9I GATC 2 cut(s) 188, 214
LmnI GCTCC 2 cut(s) 445, 458
Lsp1109I GCAGC 2 cut(s) 79, 515
LweI GCATC 1 cut(s) 346
MaeI CTAG 2 cut(s) 54, 161
MaeIII GTNAC 1 cut(s) 93
MalI GATC 2 cut(s) 190, 216
MboI GATC 2 cut(s) 188, 214
MboII GAAGA 1 cut(s) 411
MflI RGATCY 1 cut(s) 188
MluCI AATT 3 cut(s) 31, 227, 399
MnlI CCTC 6 cut(s) 115, 157, 172, 193, 437, 508
MspR9I CCNGG 1 cut(s) 193
MvaI CCWGG 1 cut(s) 193
MwoI GCNNNNNNNGC 3 cut(s) 67, 209, 467
NcoI CCATGG 2 cut(s) 7, 71
NdeII GATC 2 cut(s) 188, 214
NlaIII CATG 2 cut(s) 11, 75
NlaIV GGNNCC 2 cut(s) 190, 307
PfeI GAWTC 1 cut(s) 46
PflMI CCANNNNNTGG 1 cut(s) 315
PkrI GCNGC 2 cut(s) 69, 505
PpuMI RGGWCCY 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 191
PspFI CCCAGC 1 cut(s) 524
PspGI CCWGG 1 cut(s) 191
PspN4I GGNNCC 2 cut(s) 190, 307
PspPI GGNCC 3 cut(s) 4, 102, 306
PspPPI RGGWCCY 1 cut(s) 102
PsrI GAACNNNNNNTAC 2 cut(s) 492, 524
PsuI RGATCY 1 cut(s) 188
SatI GCNGC 2 cut(s) 68, 504
Sau3AI GATC 2 cut(s) 188, 214
Sau96I GGNCC 3 cut(s) 4, 102, 306
ScrFI CCNGG 1 cut(s) 193
SetI ASST 8 cut(s) 17, 59, 107, 116, 162, 372, 429, 442
SfaNI GCATC 1 cut(s) 346
SinI GGWCC 2 cut(s) 4, 102
SmlI CTYRAG 2 cut(s) 288, 512
SmoI CTYRAG 2 cut(s) 288, 512
Sse9I AATT 3 cut(s) 31, 227, 399
SsiI CCGC 2 cut(s) 250, 529
SspI AATATT 1 cut(s) 339
SspMI CTAG 2 cut(s) 54, 161
StyD4I CCNGG 1 cut(s) 191
StyI CCWWGG 2 cut(s) 7, 71
TasI AATT 3 cut(s) 31, 227, 399
TfiI GAWTC 1 cut(s) 46
TscAI CASTG 2 cut(s) 88, 471
TseI GCWGC 2 cut(s) 67, 503
TspDTI ATGAA 2 cut(s) 59, 135
TspRI CASTG 2 cut(s) 88, 471
Van91I CCANNNNNTGG 1 cut(s) 315
VpaK11BI GGWCC 2 cut(s) 4, 102
XapI RAATTY 1 cut(s) 227
XspI CTAG 2 cut(s) 54, 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.