RchiOBHm_Chr6g0246091

L-galactose dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011375
Physical Location & Seq
Reverse (-)
1772575 .. 1777859
5285 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22059

Sequence Viewer

Length: 1107 bp
ATGATGCTCAAATCTTGTTATAAAGTACATAATGGGCATTTCGGTTGCTGCAACCCAACCCAAGATATTTCAGTTTCCAGAGACATATTCCCCACTTCCACTATTTCACTTCCTAAATTCCTTCACAACCAAACCTCTGTCAAAGCAATGGCCAAGGTTGAACTTCGAGAACTGGGTAACACAGGGCTCAAGCTCAGCTGCGTTGGCTTCGGCGCTTCCCCTCTCGGCAACGTCTTCGGCCCAGTCTCCGACGAAGAAGCCGTCGGCTCCGTTCGTGAAGCTTTTCGCCGGGGCATCAACTTCTTCGACACCTCTCCGTACTATGGAGGGACATTGTCAGAGAAGGTGCTTGGTAAAACACTAAAGGCTCTAGGAGTTCCAAGGAGTGAGTATATTGTGGCAACAAAGTGTGGACGATATGCTGAAGGTTTCGATTTCAGTGCTGATAGAGTGACTAAGAGCATCGATGAGAGCTTGGAAAGGTTGCAGCTAGATTACGTTGATATATTGCAATGCCATGACATTGAATTTGGAAATCTCGATCAGATTGTCAATGAGACAATTCCCACTCTACAGAAACTGAAAGAAGCAGGGAAGATCCATTTCATTGGTATCACGGGACTTCCTTTGGGAGTTTTCACATATGTTCTTGACCGGGTACCACCTGGCACAGTGGACGTTGTTCTGTCATATTGCCATTACAGTATTAATGATTCAGCACTGGAGGATTTACTGCCTTACCTGAAGAGCAAAGGTGTTGGTGTAATTAGTGCTTCTCCACTTGCAATGGGGCTTCTCACTGAGCGTGGTCCTCCAGAATGGCATCCAGCATCTCCTGAACTCAAGTCTACTTGTCAAGCTGCTGCTTTGTATTGTAAAGAGAGAGGGAAGAATATTTCAAAGTTAGCTATGCAATACAGCTTGTCCAATAAGGATATCTCATCAGTGCTGGTTGGCATGAACTCTGTTAAACAGGTTGAGGAAAATGTTGCTGCTGCTGTGGAGCTTTCAACTGTTGGGAAAGATGAGCAAACCATATCAGAGGTTGAAGCTATTCTGAAACCTGTCAAGAATCAGACTTGGCCTAGTGGAATCCAGCAGAGCTAA

Protein Analysis

368

Amino Acids

40.06

Weight (kDa)

6.03

Isoelectric Point (pI)

34.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 68 - 337 3.9e-55 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011375)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33670
fragaria_vesca FvH4_2g02330 FvH4_2g02330
malus_domestica MD05G1113100.v1.1 MD10G1115600.v1.1
prunus_persica Prupe.8G157600_v2.0.a1 Prupe.8G157600_v2.0.a1
pyrus_communis pycom05g10830 pycom10g10010
rosa_chinensis RchiOBHm_Chr6g0246091
rosa_laevigata RLG00000015271
rosa_multiflora Rmu_sc0004042.1_g000009 Rmu_sc0009451.1_g000013
rosa_roxburghii Rroxscaffold_7G00214970
rosa_rugosa Rorug05G0519000
rosa_samantha Rh6AG033300 Rh6BG028800 Rh6CG029000 Rh6DG028100
rosa_wichuraiana Rw6G002680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 21
Acc65I GGTACC 1 cut(s) 658
AccB1I GGYRCC 1 cut(s) 658
AccI GTMKAC 1 cut(s) 848
AclWI GGATC 1 cut(s) 592
AcoI YGGCCR 1 cut(s) 150
AcsI RAATTY 2 cut(s) 116, 527
AcuI CTGAAG 2 cut(s) 444, 764
AfaI GTAC 3 cut(s) 27, 320, 660
AfiI CCNNNNNNNGG 1 cut(s) 323
AgsI TTSAA 5 cut(s) 161, 527, 900, 1011, 1049
AjnI CCWGG 1 cut(s) 664
Alw26I GTCTC 3 cut(s) 75, 250, 551
AlwI GGATC 1 cut(s) 592
AlwNI CAGNNNCTG 1 cut(s) 580
AoxI GGCC 3 cut(s) 150, 238, 1082
ApeKI GCWGC 7 cut(s) 48, 198, 487, 860, 863, 992, 995
ApoI RAATTY 2 cut(s) 116, 527
ArsI GACNNNNNNTTYG 4 cut(s) 246, 278, 512, 544
AseI ATTAAT 1 cut(s) 708
Asp700I GAANNNNTTC 2 cut(s) 282, 1053
Asp718I GGTACC 1 cut(s) 658
AspLEI GCGC 1 cut(s) 215
AspS9I GGNCC 2 cut(s) 239, 809
AsuC2I CCSGG 2 cut(s) 290, 656
AvaII GGWCC 1 cut(s) 809
BalI TGGCCA 1 cut(s) 152
BanI GGYRCC 1 cut(s) 658
BanII GRGCYC 1 cut(s) 189
BbsI GAAGAC 1 cut(s) 226
BbvI GCAGC 7 cut(s) 35, 185, 499, 847, 850, 979, 982
BceAI ACGGC 1 cut(s) 245
BcgI CGANNNNNNTGC 4 cut(s) 217, 251, 422, 456
BciT130I CCWGG 1 cut(s) 666
BcnI CCSGG 2 cut(s) 290, 656
BcoDI GTCTC 3 cut(s) 75, 250, 551
BfaI CTAG 3 cut(s) 371, 491, 1086
BfmI CTRYAG 1 cut(s) 572
BfoI RGCGCY 1 cut(s) 216
BisI GCNGC 7 cut(s) 49, 199, 488, 861, 864, 993, 996
BlpI GCTNAGC 1 cut(s) 194
BlsI GCNGC 7 cut(s) 50, 200, 489, 862, 865, 994, 997
Bme1390I CCNGG 3 cut(s) 290, 656, 666
Bme18I GGWCC 1 cut(s) 809
BmgT120I GGNCC 2 cut(s) 239, 809
BmiI GGNNCC 2 cut(s) 268, 660
BmrFI CCNGG 3 cut(s) 290, 656, 666
BmrI ACTGGG 2 cut(s) 182, 236
BmsI GCATC 4 cut(s) 303, 471, 832, 839
BmuI ACTGGG 2 cut(s) 182, 236
BpiI GAAGAC 1 cut(s) 226
BpmI CTGGAG 2 cut(s) 743, 798
Bpu1102I GCTNAGC 1 cut(s) 194
BpuEI CTTGAG 2 cut(s) 173, 827
BpuMI CCSGG 2 cut(s) 290, 656
Bsa29I ATCGAT 1 cut(s) 465
BsaJI CCNNGG 3 cut(s) 153, 289, 380
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse1I ACTGG 3 cut(s) 177, 242, 726
Bse3DI GCAATG 3 cut(s) 153, 518, 792
BseBI CCWGG 1 cut(s) 666
BseCI ATCGAT 1 cut(s) 465
BseDI CCNNGG 3 cut(s) 153, 289, 380
BseGI GGATG 1 cut(s) 823
BseLI CCNNNNNNNGG 1 cut(s) 323
BseMI GCAATG 3 cut(s) 153, 518, 792
BseMII CTCAG 2 cut(s) 208, 792
BseNI ACTGG 3 cut(s) 177, 242, 726
BseXI GCAGC 7 cut(s) 35, 185, 499, 847, 850, 979, 982
BshFI GGCC 3 cut(s) 152, 240, 1084
BshNI GGYRCC 1 cut(s) 658
BshVI ATCGAT 1 cut(s) 465
BsiSI CCGG 2 cut(s) 289, 655
BslFI GGGAC 2 cut(s) 343, 633
BslI CCNNNNNNNGG 1 cut(s) 323
BsmAI GTCTC 3 cut(s) 75, 250, 551
BsmFI GGGAC 2 cut(s) 343, 633
BsnI GGCC 3 cut(s) 152, 240, 1084
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 2 cut(s) 541, 597
Bsp1720I GCTNAGC 1 cut(s) 194
BspANI GGCC 3 cut(s) 152, 240, 1084
BspCNI CTCAG 2 cut(s) 207, 793
BspDI ATCGAT 1 cut(s) 465
BspLI GGNNCC 2 cut(s) 268, 660
BspPI GGATC 1 cut(s) 592
BspQI GCTCTTC 1 cut(s) 740
BspT107I GGYRCC 1 cut(s) 658
BsrDI GCAATG 3 cut(s) 153, 518, 792
BsrI ACTGG 3 cut(s) 177, 242, 726
BssECI CCNNGG 3 cut(s) 153, 289, 380
BssMI GATC 2 cut(s) 541, 597
BssT1I CCWWGG 2 cut(s) 153, 380
Bst2UI CCWGG 1 cut(s) 666
Bst4CI ACNGT 3 cut(s) 673, 704, 1015
Bst6I CTCTTC 1 cut(s) 740
BstDEI CTNAG 3 cut(s) 194, 456, 801
BstF5I GGATG 1 cut(s) 823
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 1 cut(s) 215
BstKTI GATC 2 cut(s) 544, 600
BstMAI GTCTC 3 cut(s) 75, 250, 551
BstMBI GATC 2 cut(s) 541, 597
BstMWI GCNNNNNNNGC 1 cut(s) 204
BstNI CCWGG 1 cut(s) 666
BstSCI CCNGG 3 cut(s) 288, 654, 664
BstSFI CTRYAG 1 cut(s) 572
BstV1I GCAGC 7 cut(s) 35, 185, 499, 847, 850, 979, 982
BstV2I GAAGAC 1 cut(s) 226
BstX2I RGATCY 1 cut(s) 597
BstXI CCANNNNNNTGG 1 cut(s) 608
BstYI RGATCY 1 cut(s) 597
Bsu15I ATCGAT 1 cut(s) 465
BsuRI GGCC 3 cut(s) 152, 240, 1084
BsuTUI ATCGAT 1 cut(s) 465
BtsCI GGATG 1 cut(s) 823
BtsIMutI CAGTG 5 cut(s) 445, 678, 719, 798, 951
CaiI CAGNNNCTG 1 cut(s) 580
CfoI GCGC 1 cut(s) 215
Cfr13I GGNCC 2 cut(s) 239, 809
ClaI ATCGAT 1 cut(s) 465
Csp6I GTAC 3 cut(s) 26, 319, 659
CviAII CATG 2 cut(s) 518, 958
CviQI GTAC 3 cut(s) 26, 319, 659
DdeI CTNAG 3 cut(s) 194, 456, 801
DpnI GATC 2 cut(s) 543, 599
DpnII GATC 2 cut(s) 541, 597
EaeI YGGCCR 1 cut(s) 150
Eam1104I CTCTTC 1 cut(s) 740
EarI CTCTTC 1 cut(s) 740
Eco130I CCWWGG 2 cut(s) 153, 380
Eco24I GRGCYC 1 cut(s) 189
Eco32I GATATC 1 cut(s) 937
Eco47I GGWCC 1 cut(s) 809
Eco57I CTGAAG 2 cut(s) 444, 764
EcoRII CCWGG 1 cut(s) 664
EcoRV GATATC 1 cut(s) 937
EcoT14I CCWWGG 2 cut(s) 153, 380
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 2 cut(s) 153, 380
FaeI CATG 2 cut(s) 521, 961
FaqI GGGAC 2 cut(s) 343, 633
FatI CATG 2 cut(s) 517, 957
FauNDI CATATG 1 cut(s) 643
FblI GTMKAC 1 cut(s) 848
Fnu4HI GCNGC 7 cut(s) 49, 199, 488, 861, 864, 993, 996
FokI GGATG 1 cut(s) 810
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 7 cut(s) 49, 199, 488, 861, 864, 993, 996
FspBI CTAG 3 cut(s) 371, 491, 1086
GlaI GCGC 1 cut(s) 214
GluI GCNGC 7 cut(s) 49, 199, 488, 861, 864, 993, 996
GsuI CTGGAG 2 cut(s) 743, 798
HaeII RGCGCY 1 cut(s) 216
HaeIII GGCC 3 cut(s) 152, 240, 1084
HapII CCGG 2 cut(s) 289, 655
HhaI GCGC 1 cut(s) 215
Hin1II CATG 2 cut(s) 521, 961
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 3 cut(s) 713, 1072, 1092
HpaII CCGG 2 cut(s) 289, 655
Hpy166II GTNNAC 3 cut(s) 413, 676, 849
Hpy188I TCNGA 6 cut(s) 250, 340, 546, 1042, 1059, 1077
Hpy188III TCNNGA 8 cut(s) 78, 167, 275, 539, 650, 815, 836, 1069
Hpy8I GTNNAC 3 cut(s) 413, 676, 849
Hpy99I CGWCG 2 cut(s) 254, 266
HpyAV CCTTC 3 cut(s) 131, 337, 419
HpyCH4III ACNGT 3 cut(s) 673, 704, 1015
HpyCH4IV ACGT 3 cut(s) 231, 498, 678
HpyCH4V TGCA 5 cut(s) 51, 487, 511, 785, 913
HpyF10VI GCNNNNNNNGC 1 cut(s) 204
HpyF3I CTNAG 3 cut(s) 194, 456, 801
HpySE526I ACGT 3 cut(s) 231, 498, 678
Hsp92II CATG 2 cut(s) 521, 961
HspAI GCGC 1 cut(s) 213
KpnI GGTACC 1 cut(s) 662
Kzo9I GATC 2 cut(s) 541, 597
LguI GCTCTTC 1 cut(s) 740
LmnI GCTCC 2 cut(s) 272, 1003
Lsp1109I GCAGC 7 cut(s) 35, 185, 499, 847, 850, 979, 982
LweI GCATC 4 cut(s) 303, 471, 832, 839
MaeI CTAG 3 cut(s) 371, 491, 1086
MaeII ACGT 3 cut(s) 231, 498, 678
MaeIII GTNAC 2 cut(s) 176, 451
MalI GATC 2 cut(s) 543, 599
MboI GATC 2 cut(s) 541, 597
MboII GAAGA 6 cut(s) 226, 266, 295, 607, 757, 901
MflI RGATCY 1 cut(s) 597
MhlI GDGCHC 1 cut(s) 189
MlsI TGGCCA 1 cut(s) 152
MluCI AATT 4 cut(s) 116, 527, 561, 765
MluNI TGGCCA 1 cut(s) 152
MmeI TCCRAC 1 cut(s) 273
MnlI CCTC 9 cut(s) 145, 231, 320, 322, 718, 822, 878, 973, 1036
Mox20I TGGCCA 1 cut(s) 152
MroXI GAANNNNTTC 2 cut(s) 282, 1053
MscI TGGCCA 1 cut(s) 152
MseI TTAA 2 cut(s) 708, 969
Msp20I TGGCCA 1 cut(s) 152
MspA1I CMGCKG 1 cut(s) 198
MspI CCGG 2 cut(s) 289, 655
MspR9I CCNGG 3 cut(s) 290, 656, 666
MvaI CCWGG 1 cut(s) 666
MwoI GCNNNNNNNGC 1 cut(s) 204
NciI CCSGG 2 cut(s) 290, 656
NdeI CATATG 1 cut(s) 643
NdeII GATC 2 cut(s) 541, 597
NlaIII CATG 2 cut(s) 521, 961
NlaIV GGNNCC 2 cut(s) 268, 660
NmeAIII GCCGAG 1 cut(s) 204
NmuCI GTSAC 1 cut(s) 451
PciSI GCTCTTC 1 cut(s) 740
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PdmI GAANNNNTTC 2 cut(s) 282, 1053
PfeI GAWTC 3 cut(s) 713, 1072, 1092
PflFI GACNNNGTC 1 cut(s) 334
PkrI GCNGC 7 cut(s) 50, 200, 489, 862, 865, 994, 997
PshBI ATTAAT 1 cut(s) 708
PsiI TTATAA 1 cut(s) 21
Psp6I CCWGG 1 cut(s) 664
PspGI CCWGG 1 cut(s) 664
PspN4I GGNNCC 2 cut(s) 268, 660
PspPI GGNCC 2 cut(s) 239, 809
PstNI CAGNNNCTG 1 cut(s) 580
PsuI RGATCY 1 cut(s) 597
PsyI GACNNNGTC 1 cut(s) 334
PvuII CAGCTG 1 cut(s) 198
RsaI GTAC 3 cut(s) 27, 320, 660
RsaNI GTAC 3 cut(s) 26, 319, 659
SapI GCTCTTC 1 cut(s) 740
SaqAI TTAA 2 cut(s) 708, 969
SatI GCNGC 7 cut(s) 49, 199, 488, 861, 864, 993, 996
Sau3AI GATC 2 cut(s) 541, 597
Sau96I GGNCC 2 cut(s) 239, 809
ScrFI CCNGG 3 cut(s) 290, 656, 666
SduI GDGCHC 1 cut(s) 189
SfaNI GCATC 4 cut(s) 303, 471, 832, 839
SfcI CTRYAG 1 cut(s) 572
SinI GGWCC 1 cut(s) 809
SmlI CTYRAG 2 cut(s) 188, 842
SmoI CTYRAG 2 cut(s) 188, 842
Sse9I AATT 4 cut(s) 116, 527, 561, 765
SspI AATATT 1 cut(s) 895
SspMI CTAG 3 cut(s) 371, 491, 1086
StyD4I CCNGG 3 cut(s) 288, 654, 664
StyI CCWWGG 2 cut(s) 153, 380
TaaI ACNGT 3 cut(s) 673, 704, 1015
TaiI ACGT 3 cut(s) 234, 501, 681
TaqI TCGA 5 cut(s) 166, 306, 432, 465, 540
TasI AATT 4 cut(s) 116, 527, 561, 765
TatI WGTACW 1 cut(s) 25
TfiI GAWTC 3 cut(s) 713, 1072, 1092
Tru1I TTAA 2 cut(s) 708, 969
Tru9I TTAA 2 cut(s) 708, 969
TscAI CASTG 5 cut(s) 445, 678, 726, 805, 951
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 7 cut(s) 48, 198, 487, 860, 863, 992, 995
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 2 cut(s) 595, 974
TspGWI ACGGA 2 cut(s) 259, 306
TspRI CASTG 5 cut(s) 445, 678, 726, 805, 951
Tth111I GACNNNGTC 1 cut(s) 334
VpaK11BI GGWCC 1 cut(s) 809
VspI ATTAAT 1 cut(s) 708
XapI RAATTY 2 cut(s) 116, 527
XmiI GTMKAC 1 cut(s) 848
XmnI GAANNNNTTC 2 cut(s) 282, 1053
XspI CTAG 3 cut(s) 371, 491, 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.