RchiOBHm_Chr6g0290421

Type I inositol 1,4,5-trisphosphate 5-phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0012832
Physical Location & Seq
Forward (+)
53431770 .. 53436854
5085 bp
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UTR
Exon/CDS
Intron
PRQ26056

Sequence Viewer

Length: 1677 bp
ATGATAGATGGGAATTCAAAGAAAAGCAAGCTTTCATGGCCCAAGACACTGGTCAAGAAGTGGCTCAACATCAAGAGCAAAGCTGAGGATTTTCACGCAGACGATGTTGTTTATGGAGGTGGTGATGAAGAGTGGAGGAACAACTATTCAGAGAGGGAAGCATGCACAATCAAGAAAAGCAAAACAGAAAGGTTAAGCAAGAGGCACTCTGATAAAGTCCGGCGAGGTAAGATTGACATGGACGTATCACAGGTTACAGACGTACAAAACTATAGGATTTTTGTAGCTACATGGAATGTGGCTGGAAAATCTCCTCCGAGTTGTTTGAATCTAGAAGATTGGCTTCATACCTCTCCTCCTGCTGACATTTATGTTCTAGGGTTTCAAGAAATAGTTCCTTTGAATGCTGGCAATGTGTTGGGGACAGAGGACAATGCCCCGGCTAAAAAATGGGTAGCCCTTATAAGGAAGACTCTAAATAGTCTTCCCGGCACAAGTGGTGGTTGCCATACACCTTCGCCAGTTCCTGATCCAATTGTGGAGTTGGATTCAGACTTTGAGGGATCAACAAGGCAGAAGGCCTCCTCCTTCTTCCACCGCCGGTCCTTTCAATCCTTGAGCCGCAGCATGAGAATGGAGAATGACATGTCAATGCCACAACCCCAGCTTGATCGACGGTACAGTGTTTGTGATCGAGTTATTTTTGGGAATAGACCGAGTGACTATGACCCCAATGTAAGATGGGGTTCCTCTGATGATGATAATGGACCTGGTGAGTCACCTGTTGTCACACAGTATTCACCAATGTCCAATAGTGGATCATTTTCAATAGAGGATAGAGAAAGACAGCAAGGGAACTCAAGGTACTGCTTGGTTGCCAGCAAGCAAATGGTTGGGATATTTTTAACGGTGTGGGTGAGAAGTGATCTCAGAGATGATGTTCGCAATATGAAGGTGTCTTGTGTTGGCAGAGGATTGATGGGCTATCTTGGAAATAAGGGTTCAATTTCAATTAGCATGTCCTTGCACCAAACAAGCTTCTGCTTCATCTGTAGTCATCTAACCTCTGGGCAGAAGGACGGAGATGAACTTCGAAGAAACTCTGATGTCTTGGAGATCCTTAGGAAGACAAGGTTTCCCAGGGTTAATGGAATGGGAGATGAAAGCTCTCCTCAGACTATACTAGAGCATGATCGGATCATTTGGCTCGGTGATTTGAATTATCGCATTGCCCTTTCCTACCGCTATGCAAAAGCTCTTGTTGAGATGCGCAACTGGAGGGCATTGTTAGAGAAGGACCAGCTGAGGATAGAGCAGAGACGAGGACGTGTCTTTGGGGGATGGAGTGAAGGGAAGATATATTTCCCTCCGACATACAAGTATTTGAACAATTCAGATAGGTATGCCGGTGAAGATAGGCACAACAAGGAGAAGCGAAGAACTCCAGCATGGTGTGATCGTATATTATGGCATGGAAGAGGCCTCCTTCAATTGTCCTATGTTCGTGGGGAGTCGAGGTTCTCAGATCACAGGCCAGTTTACAGCGTATTTCTGGCAGAGGTCGAGTCCATTAACCGTAACCGAATCAAGAAAAGCATGAGTTGTTCCAGTTCCAGGATTGAGGTAGAGGAGCTGTTGCCACACTCGTGTGGATACTCCCAACTAAATTTCTATTGA

Protein Analysis

558

Amino Acids

63.91

Weight (kDa)

9.15

Isoelectric Point (pI)

58.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos2 PF22669 97 - 161 2e-07 Endonuclease/Exonuclease/phosphatase family 2
Exo_endo_phos2 PF22669 282 - 515 6.2e-63 Endonuclease/Exonuclease/phosphatase family 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63240 AT3G63240
fragaria_vesca FvH4_2g23170
malus_domestica MD03G1261100.v1.1 MD11G1281400.v1.1
prunus_persica Prupe.8G236900_v2.0.a1
pyrus_communis pycom03g20850 pycom11g24940
rosa_chinensis RchiOBHm_Chr6g0290421
rosa_laevigata RLG00000012220
rosa_multiflora Rmu_sc0002890.1_g000001
rosa_roxburghii Rroxscaffold_7G00177080
rosa_rugosa Rorug06G0212600
rosa_samantha Rh6AG322600 Rh6BG330100 Rh6CG336100
rosa_wichuraiana Rw6G027880 Rw6G027970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 464
Acc16I TGCGCA 1 cut(s) 1271
AciI CCGC 3 cut(s) 598, 622, 1243
AclWI GGATC 5 cut(s) 524, 571, 826, 1111, 1205
AcsI RAATTY 2 cut(s) 13, 1666
AfaI GTAC 3 cut(s) 264, 680, 866
AfiI CCNNNNNNNGG 2 cut(s) 465, 1614
AflIII ACRYGT 2 cut(s) 645, 1327
AjiI CACGTC 1 cut(s) 1328
AjnI CCWGG 3 cut(s) 769, 1139, 1613
AleI CACNNNNGTG 2 cut(s) 1645, 1647
AloI GAACNNNNNNTCC 2 cut(s) 1502, 1534
AluBI AGCT 9 cut(s) 31, 83, 287, 667, 1038, 1167, 1256, 1303, 1633
AluI AGCT 9 cut(s) 31, 83, 287, 667, 1038, 1167, 1256, 1303, 1633
Alw26I GTCTC 1 cut(s) 1312
AlwI GGATC 5 cut(s) 524, 571, 826, 1111, 1205
AlwNI CAGNNNCTG 1 cut(s) 527
AoxI GGCC 4 cut(s) 38, 579, 1480, 1532
ApeKI GCWGC 1 cut(s) 624
ApoI RAATTY 2 cut(s) 13, 1666
Asp700I GAANNNNTTC 1 cut(s) 393
AspLEI GCGC 1 cut(s) 1272
AspS9I GGNCC 4 cut(s) 39, 603, 767, 1297
AsuC2I CCSGG 2 cut(s) 440, 489
AsuHPI GGTGA 7 cut(s) 134, 771, 785, 792, 928, 1223, 1421
AsuII TTCGAA 1 cut(s) 1093
AvaII GGWCC 3 cut(s) 603, 767, 1297
AxyI CCTNAGG 1 cut(s) 1121
BauI CACGAG 1 cut(s) 1645
BbsI GAAGAC 3 cut(s) 476, 476, 1133
BbvCI CCTCAGC 2 cut(s) 84, 1304
BbvI GCAGC 1 cut(s) 636
BccI CCATC 4 cut(s) 2, 735, 973, 1335
BciT130I CCWGG 3 cut(s) 771, 1141, 1615
BciVI GTATCC 1 cut(s) 1646
BcnI CCSGG 2 cut(s) 440, 489
BcoDI GTCTC 1 cut(s) 1312
BfaI CTAG 3 cut(s) 332, 377, 1184
BfmI CTRYAG 2 cut(s) 271, 1051
BfuI GTATCC 1 cut(s) 1646
BisI GCNGC 2 cut(s) 622, 625
BlsI GCNGC 2 cut(s) 623, 626
Bme1390I CCNGG 5 cut(s) 440, 489, 771, 1141, 1615
Bme18I GGWCC 3 cut(s) 603, 767, 1297
BmgBI CACGTC 1 cut(s) 1328
BmgT120I GGNCC 4 cut(s) 39, 603, 767, 1297
BmiI GGNNCC 1 cut(s) 748
BmrFI CCNGG 5 cut(s) 440, 489, 771, 1141, 1615
BmsI GCATC 1 cut(s) 1257
BoxI GACNNNNGTC 1 cut(s) 50
BpiI GAAGAC 3 cut(s) 476, 476, 1133
BpmI CTGGAG 2 cut(s) 1297, 1428
Bpu10I CCTNAGC 2 cut(s) 84, 1304
Bpu14I TTCGAA 1 cut(s) 1093
BpuEI CTTGAG 2 cut(s) 637, 844
BpuMI CCSGG 2 cut(s) 440, 489
BsaJI CCNNGG 3 cut(s) 438, 1139, 1140
BsaXI ACNNNNNCTCC 4 cut(s) 340, 370, 1502, 1532
Bsc4I CCNNNNNNNGG 2 cut(s) 465, 1614
Bse118I RCCGGY 2 cut(s) 600, 1406
Bse1I ACTGG 5 cut(s) 54, 521, 1280, 1535, 1608
Bse21I CCTNAGG 1 cut(s) 1121
Bse3DI GCAATG 2 cut(s) 418, 1227
BseBI CCWGG 3 cut(s) 771, 1141, 1615
BseDI CCNNGG 3 cut(s) 438, 1139, 1140
BseGI GGATG 1 cut(s) 1346
BseLI CCNNNNNNNGG 2 cut(s) 465, 1614
BseMI GCAATG 2 cut(s) 418, 1227
BseMII CTCAG 5 cut(s) 75, 943, 1187, 1295, 1536
BseNI ACTGG 5 cut(s) 54, 521, 1280, 1535, 1608
BseRI GAGGAG 5 cut(s) 303, 345, 574, 1161, 1643
BseXI GCAGC 1 cut(s) 636
BseYI CCCAGC 1 cut(s) 663
BshFI GGCC 4 cut(s) 40, 581, 1482, 1534
BsiSI CCGG 5 cut(s) 220, 440, 489, 601, 1407
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 2 cut(s) 465, 1614
BsmAI GTCTC 1 cut(s) 1312
BsmBI CGTCTC 1 cut(s) 1312
BsmFI GGGAC 1 cut(s) 436
BsmI GAATGC 1 cut(s) 409
BsnI GGCC 4 cut(s) 40, 581, 1482, 1534
Bsp119I TTCGAA 1 cut(s) 1093
BspACI CCGC 3 cut(s) 598, 622, 1243
BspANI GGCC 4 cut(s) 40, 581, 1482, 1534
BspCNI CTCAG 5 cut(s) 76, 942, 1186, 1296, 1535
BspLI GGNNCC 1 cut(s) 748
BspPI GGATC 5 cut(s) 524, 571, 826, 1111, 1205
BspT104I TTCGAA 1 cut(s) 1093
BsrDI GCAATG 2 cut(s) 418, 1227
BsrFI RCCGGY 2 cut(s) 600, 1406
BsrI ACTGG 5 cut(s) 54, 521, 1280, 1535, 1608
BssAI RCCGGY 2 cut(s) 600, 1406
BssECI CCNNGG 3 cut(s) 438, 1139, 1140
BssSI CACGAG 1 cut(s) 1645
Bst2BI CACGAG 1 cut(s) 1645
Bst2UI CCWGG 3 cut(s) 771, 1141, 1615
Bst4CI ACNGT 5 cut(s) 678, 683, 795, 910, 1577
Bst6I CTCTTC 2 cut(s) 123, 1471
BstBI TTCGAA 1 cut(s) 1093
BstC8I GCNNGC 5 cut(s) 29, 163, 409, 880, 884
BstDEI CTNAG 6 cut(s) 84, 929, 1121, 1173, 1304, 1522
BstF5I GGATG 1 cut(s) 1346
BstHHI GCGC 1 cut(s) 1272
BstMAI GTCTC 1 cut(s) 1312
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstNI CCWGG 3 cut(s) 771, 1141, 1615
BstNSI RCATGY 3 cut(s) 165, 649, 1021
BstPAI GACNNNNGTC 1 cut(s) 50
BstSCI CCNGG 5 cut(s) 438, 487, 769, 1139, 1613
BstSFI CTRYAG 2 cut(s) 271, 1051
BstV1I GCAGC 1 cut(s) 636
BstV2I GAAGAC 3 cut(s) 476, 476, 1133
BstX2I RGATCY 1 cut(s) 1116
BstXI CCANNNNNNTGG 1 cut(s) 49
BstYI RGATCY 1 cut(s) 1116
Bsu36I CCTNAGG 1 cut(s) 1121
BsuI GTATCC 1 cut(s) 1646
BsuRI GGCC 4 cut(s) 40, 581, 1482, 1534
BtrI CACGTC 1 cut(s) 1328
BtsCI GGATG 1 cut(s) 1346
BtsIMutI CAGTG 2 cut(s) 47, 688
Cac8I GCNNGC 5 cut(s) 29, 163, 409, 880, 884
CaiI CAGNNNCTG 1 cut(s) 527
CfoI GCGC 1 cut(s) 1272
Cfr10I RCCGGY 2 cut(s) 600, 1406
Cfr13I GGNCC 4 cut(s) 39, 603, 767, 1297
CsiI ACCWGGT 1 cut(s) 769
Csp6I GTAC 3 cut(s) 263, 679, 865
CviQI GTAC 3 cut(s) 263, 679, 865
DdeI CTNAG 6 cut(s) 84, 929, 1121, 1173, 1304, 1522
Eam1104I CTCTTC 2 cut(s) 123, 1471
EarI CTCTTC 2 cut(s) 123, 1471
Eco147I AGGCCT 2 cut(s) 581, 1482
Eco47I GGWCC 3 cut(s) 603, 767, 1297
Eco81I CCTNAGG 1 cut(s) 1121
EcoRI GAATTC 1 cut(s) 13
EcoRII CCWGG 3 cut(s) 769, 1139, 1613
Esp3I CGTCTC 1 cut(s) 1312
FalI AAGNNNNNCTT 2 cut(s) 327, 359
FaqI GGGAC 1 cut(s) 436
Fnu4HI GCNGC 2 cut(s) 622, 625
FokI GGATG 1 cut(s) 1353
Fsp4HI GCNGC 2 cut(s) 622, 625
FspBI CTAG 3 cut(s) 332, 377, 1184
FspI TGCGCA 1 cut(s) 1271
GlaI GCGC 1 cut(s) 1271
GluI GCNGC 2 cut(s) 622, 625
GsaI CCCAGC 1 cut(s) 667
GsuI CTGGAG 2 cut(s) 1297, 1428
HaeIII GGCC 4 cut(s) 40, 581, 1482, 1534
HapII CCGG 5 cut(s) 220, 440, 489, 601, 1407
HhaI GCGC 1 cut(s) 1272
Hin6I GCGC 1 cut(s) 1270
HinP1I GCGC 1 cut(s) 1270
HindIII AAGCTT 2 cut(s) 29, 1036
HinfI GANTC 7 cut(s) 328, 472, 548, 776, 1511, 1565, 1584
HpaII CCGG 5 cut(s) 220, 440, 489, 601, 1407
HphI GGTGA 7 cut(s) 134, 771, 785, 792, 928, 1223, 1421
Hpy166II GTNNAC 1 cut(s) 1540
Hpy188III TCNNGA 7 cut(s) 55, 73, 172, 332, 386, 527, 1588
Hpy8I GTNNAC 1 cut(s) 1540
Hpy99I CGWCG 1 cut(s) 678
HpyAV CCTTC 8 cut(s) 525, 571, 598, 946, 1069, 1288, 1343, 1496
HpyCH4III ACNGT 5 cut(s) 678, 683, 795, 910, 1577
HpyCH4IV ACGT 3 cut(s) 243, 261, 1327
HpyCH4V TGCA 3 cut(s) 165, 1027, 1250
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 6 cut(s) 84, 929, 1121, 1173, 1304, 1522
HpySE526I ACGT 3 cut(s) 243, 261, 1327
HspAI GCGC 1 cut(s) 1270
LmnI GCTCC 1 cut(s) 1630
Lsp1109I GCAGC 1 cut(s) 636
LweI GCATC 1 cut(s) 1257
MabI ACCWGGT 1 cut(s) 769
MaeI CTAG 3 cut(s) 332, 377, 1184
MaeII ACGT 3 cut(s) 243, 261, 1327
MaeIII GTNAC 5 cut(s) 253, 719, 777, 787, 1577
MfeI CAATTG 2 cut(s) 534, 1490
MflI RGATCY 1 cut(s) 1116
MluCI AATT 8 cut(s) 13, 534, 1005, 1011, 1219, 1390, 1490, 1666
MlyI GAGTC 4 cut(s) 466, 785, 1520, 1574
MmeI TCCRAC 2 cut(s) 525, 1394
MroXI GAANNNNTTC 1 cut(s) 393
MseI TTAA 4 cut(s) 194, 905, 1146, 1572
MslI CAYNNNNRTG 4 cut(s) 632, 650, 1645, 1647
MspA1I CMGCKG 1 cut(s) 1303
MspI CCGG 5 cut(s) 220, 440, 489, 601, 1407
MspR9I CCNGG 5 cut(s) 440, 489, 771, 1141, 1615
MunI CAATTG 2 cut(s) 534, 1490
Mva1269I GAATGC 1 cut(s) 409
MvaI CCWGG 3 cut(s) 771, 1141, 1615
MwoI GCNNNNNNNGC 1 cut(s) 37
NciI CCSGG 2 cut(s) 440, 489
NlaIV GGNNCC 1 cut(s) 748
NmuCI GTSAC 3 cut(s) 719, 777, 787
NsbI TGCGCA 1 cut(s) 1271
NspI RCATGY 3 cut(s) 165, 649, 1021
NspV TTCGAA 1 cut(s) 1093
OliI CACNNNNGTG 2 cut(s) 1645, 1647
PaeI GCATGC 1 cut(s) 165
PasI CCCWGGG 1 cut(s) 1140
PceI AGGCCT 2 cut(s) 581, 1482
PciI ACATGT 1 cut(s) 645
PctI GAATGC 1 cut(s) 409
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 3 cut(s) 328, 548, 1584
PfoI TCCNGGA 1 cut(s) 1613
PkrI GCNGC 2 cut(s) 623, 626
PleI GAGTC 4 cut(s) 466, 784, 1519, 1573
PpsI GAGTC 4 cut(s) 466, 784, 1519, 1573
PscI ACATGT 1 cut(s) 645
PshAI GACNNNNGTC 1 cut(s) 50
PsiI TTATAA 1 cut(s) 464
Psp6I CCWGG 3 cut(s) 769, 1139, 1613
PspFI CCCAGC 1 cut(s) 663
PspGI CCWGG 3 cut(s) 769, 1139, 1613
PspN4I GGNNCC 1 cut(s) 748
PspPI GGNCC 4 cut(s) 39, 603, 767, 1297
PsrI GAACNNNNNNTAC 2 cut(s) 848, 880
PstNI CAGNNNCTG 1 cut(s) 527
PsuI RGATCY 1 cut(s) 1116
PvuII CAGCTG 1 cut(s) 1303
RsaI GTAC 3 cut(s) 264, 680, 866
RsaNI GTAC 3 cut(s) 263, 679, 865
RseI CAYNNNNRTG 4 cut(s) 632, 650, 1645, 1647
SaqAI TTAA 4 cut(s) 194, 905, 1146, 1572
SatI GCNGC 2 cut(s) 622, 625
Sau96I GGNCC 4 cut(s) 39, 603, 767, 1297
SchI GAGTC 4 cut(s) 466, 785, 1520, 1574
ScrFI CCNGG 5 cut(s) 440, 489, 771, 1141, 1615
SexAI ACCWGGT 1 cut(s) 769
SfaNI GCATC 1 cut(s) 1257
SfcI CTRYAG 2 cut(s) 271, 1051
SfuI TTCGAA 1 cut(s) 1093
SinI GGWCC 3 cut(s) 603, 767, 1297
SmiMI CAYNNNNRTG 4 cut(s) 632, 650, 1645, 1647
SmlI CTYRAG 2 cut(s) 616, 859
SmoI CTYRAG 2 cut(s) 616, 859
SphI GCATGC 1 cut(s) 165
Sse9I AATT 8 cut(s) 13, 534, 1005, 1011, 1219, 1390, 1490, 1666
SseBI AGGCCT 2 cut(s) 581, 1482
SsiI CCGC 3 cut(s) 598, 622, 1243
SspMI CTAG 3 cut(s) 332, 377, 1184
StuI AGGCCT 2 cut(s) 581, 1482
StyD4I CCNGG 5 cut(s) 438, 487, 769, 1139, 1613
TaaI ACNGT 5 cut(s) 678, 683, 795, 910, 1577
TaiI ACGT 3 cut(s) 246, 264, 1330
TaqI TCGA 5 cut(s) 673, 694, 1093, 1514, 1563
TaqII GACCGA 1 cut(s) 730
TasI AATT 8 cut(s) 13, 534, 1005, 1011, 1219, 1390, 1490, 1666
TauI GCSGC 1 cut(s) 624
TfiI GAWTC 3 cut(s) 328, 548, 1584
Tru1I TTAA 4 cut(s) 194, 905, 1146, 1572
Tru9I TTAA 4 cut(s) 194, 905, 1146, 1572
TscAI CASTG 2 cut(s) 54, 688
TseFI GTSAC 3 cut(s) 719, 777, 787
TseI GCWGC 1 cut(s) 624
Tsp45I GTSAC 3 cut(s) 719, 777, 787
TspDTI ATGAA 7 cut(s) 24, 141, 335, 965, 1036, 1101, 1176
TspGWI ACGGA 1 cut(s) 1095
TspRI CASTG 2 cut(s) 54, 688
VpaK11BI GGWCC 3 cut(s) 603, 767, 1297
XapI RAATTY 2 cut(s) 13, 1666
XbaI TCTAGA 1 cut(s) 331
XceI RCATGY 3 cut(s) 165, 649, 1021
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 3 cut(s) 332, 377, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.