Rh6BG330100

Type I inositol 1,4,5-trisphosphate 5-phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0012832
Physical Location & Seq
Forward (+)
56305732 .. 56308857
3126 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG330100.1

Sequence Viewer

Length: 1572 bp
ATGATAGATGGGAATTCAAAGAAAAGCAAGCTTTCATGGCCCAAGACACTGGTCAAGAAGTGGCTCAACATCAAGAACAAAGCTGAGGATTTTCACGCAGACGATGTTGTTTATGGAGGTGGTGATGAAGAGTGGAGGAACAACTATTCAGAGAGGGAAGCATGCACAATCAAGAAAAGCAAAACAGAAAGGTTAAGCAAGAGGCACTCTGATAAAGTCCGGCGAGGTAAGATTGACATGGACGCATCACAGGTTACAGACGTACAAAATTATAGGTTTCAAGAAATAGTTCCTTTGAATGCTGGCAATGTGTTGGGGACAGAGGACAATGCCCCGGCTAAAAAATGGGTAGCCCTTATAAGGAAGACTCTAAATAGTCTTCCCGGCGCAAGTGGTGGTTGCCATACACCTTCGCCAGTTCCTGATCCAATTGTGGAGTTGGATTCAGACTTTGAGGGATCAACAAGGCAGAAGGCCTCCTCCTTCTTCCACCGCCGGTCCTTTCAATCCTTGAGCCGCAGCATGAGAATGGAGAATGACATGTCAATGCCACAACCCCAGCTTGATCGACGGTACAGTGTTTGTGATCGAGTTATTTTTGGGAATAGACCGAGTGACTATGACCCCAATATAAGATGGGGTTCCTCTGATGATGATAATGGACCTGGTGAGTCACCTGTTGTCACACAGTATTCACCAATGTCCAATAGTGGATCATTTTCGATAGAGGATAGAGAAAGACAGCAAGGGAACTCAAGGTACTGCTTGGTTGCCAGCAAGCAAATGGTTGGGATATTTTTAACGGTGTGGGTGAGAAGTGATCTCAGAGATGATGTTCGCAATATGAAGGTGTCTTGTGTTGGCAGAGGATTGATGGGCTATCTTGGAAATAAGGGTTCAATTTCAATTAGCATGTCCTTGCACCAAACAAGCTTCTGCTTCATCTGTAGTCATCTAACCTCTGGGCAGAAGGACGGAGATGAACTTCGAAGAAACTCTGATGTCTTGGAGATCCTTAGGAAGACAAGGTTTCCCAGGGTTAATGGAATGGGAGATGAAAGCTCTCCTCAGACTATACTAGAGCATGATCGAATCATTTGGCTCGGTGATTTGAATTATCGCATTGCCCTTTCTTACCGCTATGCAAAAGCTCTTGTTGAGATGCGCAACTGGAGGGCATTGTTAGAGAAGGACCAGCTAAGGATAGAGCAGAGACGAGGACGTGTCTTTGAGGGATGGAGTGAAGGGAAGATATATTTCCCTCCAACATACAAGTATTTGAACAATTCAGATAGGTATGCCGGTGATGATAGGCACAACAAGGAGAAGCGAAGAACTCCAGCATGGTGTGATCGTATATTATGGCATGGAAGAGGCCTCCTTCAATTGTCCTATGTTCGTGGGGAGTCGAGGTTCTCAGATCACAGGCCAGTTTACAGCGTATTTCTGGCAGAGGTCGAGTCCATTAACCGTAACCGAATCAAGAAAAGCATGAGTTGTTCCAGTTCCAGGATTGAGGTAGAGGAGCTGTTGCCACACTCGCGTGGATACTCCCAACTAAATTTCTATTGA

Protein Analysis

523

Amino Acids

60.21

Weight (kDa)

9.33

Isoelectric Point (pI)

57.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos2 PF22669 247 - 480 2.1e-63 Endonuclease/Exonuclease/phosphatase family 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63240 AT3G63240
fragaria_vesca FvH4_2g23170
malus_domestica MD03G1261100.v1.1 MD11G1281400.v1.1
prunus_persica Prupe.8G236900_v2.0.a1
pyrus_communis pycom03g20850 pycom11g24940
rosa_chinensis RchiOBHm_Chr6g0290421
rosa_laevigata RLG00000012220
rosa_multiflora Rmu_sc0002890.1_g000001
rosa_roxburghii Rroxscaffold_7G00177080
rosa_rugosa Rorug06G0212600
rosa_samantha Rh6AG322600 Rh6BG330100 Rh6CG336100
rosa_wichuraiana Rw6G027880 Rw6G027970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 359
Acc16I TGCGCA 1 cut(s) 1166
AccII CGCG 1 cut(s) 1543
AciI CCGC 3 cut(s) 493, 517, 1138
AclWI GGATC 4 cut(s) 419, 466, 721, 1006
AcsI RAATTY 2 cut(s) 13, 1561
AfaI GTAC 3 cut(s) 264, 575, 761
AfiI CCNNNNNNNGG 2 cut(s) 360, 1509
AflIII ACRYGT 2 cut(s) 540, 1222
AgsI TTSAA 9 cut(s) 18, 281, 298, 506, 900, 906, 1114, 1282, 1385
AjiI CACGTC 1 cut(s) 1223
AjnI CCWGG 3 cut(s) 664, 1034, 1508
AleI CACNNNNGTG 1 cut(s) 1542
AloI GAACNNNNNNTCC 2 cut(s) 1397, 1429
AluBI AGCT 8 cut(s) 31, 83, 562, 933, 1062, 1151, 1198, 1528
AluI AGCT 8 cut(s) 31, 83, 562, 933, 1062, 1151, 1198, 1528
Alw26I GTCTC 1 cut(s) 1207
AlwI GGATC 4 cut(s) 419, 466, 721, 1006
AlwNI CAGNNNCTG 1 cut(s) 422
AoxI GGCC 4 cut(s) 38, 474, 1375, 1427
ApeKI GCWGC 1 cut(s) 519
ApoI RAATTY 2 cut(s) 13, 1561
Asp700I GAANNNNTTC 1 cut(s) 288
AspLEI GCGC 2 cut(s) 389, 1167
AspS9I GGNCC 4 cut(s) 39, 498, 662, 1192
AsuC2I CCSGG 2 cut(s) 335, 384
AsuHPI GGTGA 7 cut(s) 134, 666, 680, 687, 823, 1118, 1316
AsuII TTCGAA 1 cut(s) 988
AvaII GGWCC 3 cut(s) 498, 662, 1192
AxyI CCTNAGG 1 cut(s) 1016
BbsI GAAGAC 3 cut(s) 371, 371, 1028
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 531
BccI CCATC 4 cut(s) 2, 630, 868, 1230
BciT130I CCWGG 3 cut(s) 666, 1036, 1510
BciVI GTATCC 1 cut(s) 1541
BcnI CCSGG 2 cut(s) 335, 384
BcoDI GTCTC 1 cut(s) 1207
BfaI CTAG 1 cut(s) 1079
BfmI CTRYAG 1 cut(s) 946
BfuI GTATCC 1 cut(s) 1541
BisI GCNGC 2 cut(s) 517, 520
BlsI GCNGC 2 cut(s) 518, 521
Bme1390I CCNGG 5 cut(s) 335, 384, 666, 1036, 1510
Bme18I GGWCC 3 cut(s) 498, 662, 1192
BmgBI CACGTC 1 cut(s) 1223
BmgT120I GGNCC 4 cut(s) 39, 498, 662, 1192
BmiI GGNNCC 1 cut(s) 643
BmrFI CCNGG 5 cut(s) 335, 384, 666, 1036, 1510
BmsI GCATC 2 cut(s) 254, 1152
BoxI GACNNNNGTC 1 cut(s) 50
BpiI GAAGAC 3 cut(s) 371, 371, 1028
BpmI CTGGAG 2 cut(s) 1192, 1323
Bpu10I CCTNAGC 2 cut(s) 84, 1199
Bpu14I TTCGAA 1 cut(s) 988
BpuEI CTTGAG 2 cut(s) 532, 739
BpuMI CCSGG 2 cut(s) 335, 384
BsaJI CCNNGG 3 cut(s) 333, 1034, 1035
BsaXI ACNNNNNCTCC 2 cut(s) 1397, 1427
Bsc4I CCNNNNNNNGG 2 cut(s) 360, 1509
Bse118I RCCGGY 2 cut(s) 495, 1301
Bse1I ACTGG 5 cut(s) 54, 416, 1175, 1430, 1503
Bse21I CCTNAGG 1 cut(s) 1016
Bse3DI GCAATG 2 cut(s) 313, 1122
BseBI CCWGG 3 cut(s) 666, 1036, 1510
BseDI CCNNGG 3 cut(s) 333, 1034, 1035
BseGI GGATG 1 cut(s) 1241
BseLI CCNNNNNNNGG 2 cut(s) 360, 1509
BseMI GCAATG 2 cut(s) 313, 1122
BseMII CTCAG 4 cut(s) 75, 838, 1082, 1431
BseNI ACTGG 5 cut(s) 54, 416, 1175, 1430, 1503
BseRI GAGGAG 3 cut(s) 469, 1056, 1538
BseXI GCAGC 1 cut(s) 531
BseYI CCCAGC 1 cut(s) 558
Bsh1236I CGCG 1 cut(s) 1543
BshFI GGCC 4 cut(s) 40, 476, 1377, 1429
BsiSI CCGG 5 cut(s) 220, 335, 384, 496, 1302
BslFI GGGAC 1 cut(s) 331
BslI CCNNNNNNNGG 2 cut(s) 360, 1509
BsmAI GTCTC 1 cut(s) 1207
BsmBI CGTCTC 1 cut(s) 1207
BsmFI GGGAC 1 cut(s) 331
BsmI GAATGC 1 cut(s) 304
BsnI GGCC 4 cut(s) 40, 476, 1377, 1429
Bsp119I TTCGAA 1 cut(s) 988
BspACI CCGC 3 cut(s) 493, 517, 1138
BspANI GGCC 4 cut(s) 40, 476, 1377, 1429
BspCNI CTCAG 4 cut(s) 76, 837, 1081, 1430
BspFNI CGCG 1 cut(s) 1543
BspLI GGNNCC 1 cut(s) 643
BspPI GGATC 4 cut(s) 419, 466, 721, 1006
BspT104I TTCGAA 1 cut(s) 988
BsrDI GCAATG 2 cut(s) 313, 1122
BsrFI RCCGGY 2 cut(s) 495, 1301
BsrI ACTGG 5 cut(s) 54, 416, 1175, 1430, 1503
BssAI RCCGGY 2 cut(s) 495, 1301
BssECI CCNNGG 3 cut(s) 333, 1034, 1035
Bst2UI CCWGG 3 cut(s) 666, 1036, 1510
Bst4CI ACNGT 5 cut(s) 573, 578, 690, 805, 1472
Bst6I CTCTTC 2 cut(s) 123, 1366
BstBI TTCGAA 1 cut(s) 988
BstC8I GCNNGC 5 cut(s) 29, 163, 304, 775, 779
BstDEI CTNAG 6 cut(s) 84, 824, 1016, 1068, 1199, 1417
BstF5I GGATG 1 cut(s) 1241
BstFNI CGCG 1 cut(s) 1543
BstHHI GCGC 2 cut(s) 389, 1167
BstMAI GTCTC 1 cut(s) 1207
BstMWI GCNNNNNNNGC 2 cut(s) 37, 1540
BstNI CCWGG 3 cut(s) 666, 1036, 1510
BstNSI RCATGY 3 cut(s) 165, 544, 916
BstPAI GACNNNNGTC 1 cut(s) 50
BstSCI CCNGG 5 cut(s) 333, 382, 664, 1034, 1508
BstSFI CTRYAG 1 cut(s) 946
BstUI CGCG 1 cut(s) 1543
BstV1I GCAGC 1 cut(s) 531
BstV2I GAAGAC 3 cut(s) 371, 371, 1028
BstX2I RGATCY 1 cut(s) 1011
BstXI CCANNNNNNTGG 1 cut(s) 49
BstYI RGATCY 1 cut(s) 1011
Bsu36I CCTNAGG 1 cut(s) 1016
BsuI GTATCC 1 cut(s) 1541
BsuRI GGCC 4 cut(s) 40, 476, 1377, 1429
BtrI CACGTC 1 cut(s) 1223
BtsCI GGATG 1 cut(s) 1241
BtsIMutI CAGTG 2 cut(s) 47, 583
Cac8I GCNNGC 5 cut(s) 29, 163, 304, 775, 779
CaiI CAGNNNCTG 1 cut(s) 422
CfoI GCGC 2 cut(s) 389, 1167
Cfr10I RCCGGY 2 cut(s) 495, 1301
Cfr13I GGNCC 4 cut(s) 39, 498, 662, 1192
CseI GACGC 1 cut(s) 251
CsiI ACCWGGT 1 cut(s) 664
Csp6I GTAC 3 cut(s) 263, 574, 760
CviQI GTAC 3 cut(s) 263, 574, 760
DdeI CTNAG 6 cut(s) 84, 824, 1016, 1068, 1199, 1417
Eam1104I CTCTTC 2 cut(s) 123, 1366
EarI CTCTTC 2 cut(s) 123, 1366
Eco147I AGGCCT 2 cut(s) 476, 1377
Eco47I GGWCC 3 cut(s) 498, 662, 1192
Eco81I CCTNAGG 1 cut(s) 1016
EcoRI GAATTC 1 cut(s) 13
EcoRII CCWGG 3 cut(s) 664, 1034, 1508
Esp3I CGTCTC 1 cut(s) 1207
FaqI GGGAC 1 cut(s) 331
Fnu4HI GCNGC 2 cut(s) 517, 520
FokI GGATG 1 cut(s) 1248
Fsp4HI GCNGC 2 cut(s) 517, 520
FspBI CTAG 1 cut(s) 1079
FspI TGCGCA 1 cut(s) 1166
GlaI GCGC 2 cut(s) 388, 1166
GluI GCNGC 2 cut(s) 517, 520
GsaI CCCAGC 1 cut(s) 562
GsuI CTGGAG 2 cut(s) 1192, 1323
HaeIII GGCC 4 cut(s) 40, 476, 1377, 1429
HapII CCGG 5 cut(s) 220, 335, 384, 496, 1302
HgaI GACGC 1 cut(s) 251
HhaI GCGC 2 cut(s) 389, 1167
Hin6I GCGC 2 cut(s) 387, 1165
HinP1I GCGC 2 cut(s) 387, 1165
HindIII AAGCTT 2 cut(s) 29, 931
HinfI GANTC 7 cut(s) 367, 443, 671, 1092, 1406, 1460, 1479
HpaII CCGG 5 cut(s) 220, 335, 384, 496, 1302
HphI GGTGA 7 cut(s) 134, 666, 680, 687, 823, 1118, 1316
Hpy166II GTNNAC 1 cut(s) 1435
Hpy188I TCNGA 9 cut(s) 151, 211, 448, 649, 827, 1000, 1071, 1291, 1420
Hpy188III TCNNGA 6 cut(s) 55, 73, 172, 281, 422, 1483
Hpy8I GTNNAC 1 cut(s) 1435
Hpy99I CGWCG 1 cut(s) 573
HpyAV CCTTC 8 cut(s) 420, 466, 493, 841, 964, 1183, 1238, 1391
HpyCH4III ACNGT 5 cut(s) 573, 578, 690, 805, 1472
HpyCH4IV ACGT 2 cut(s) 261, 1222
HpyCH4V TGCA 3 cut(s) 165, 922, 1145
HpyF10VI GCNNNNNNNGC 2 cut(s) 37, 1540
HpyF3I CTNAG 6 cut(s) 84, 824, 1016, 1068, 1199, 1417
HpySE526I ACGT 2 cut(s) 261, 1222
HspAI GCGC 2 cut(s) 387, 1165
LmnI GCTCC 1 cut(s) 1525
Lsp1109I GCAGC 1 cut(s) 531
LweI GCATC 2 cut(s) 254, 1152
MabI ACCWGGT 1 cut(s) 664
MaeI CTAG 1 cut(s) 1079
MaeII ACGT 2 cut(s) 261, 1222
MaeIII GTNAC 5 cut(s) 253, 614, 672, 682, 1472
MboII GAAGA 9 cut(s) 140, 371, 376, 478, 1002, 1033, 1261, 1344, 1383
MfeI CAATTG 2 cut(s) 429, 1385
MflI RGATCY 1 cut(s) 1011
MluCI AATT 9 cut(s) 13, 268, 429, 900, 906, 1114, 1285, 1385, 1561
MlyI GAGTC 4 cut(s) 361, 680, 1415, 1469
MmeI TCCRAC 2 cut(s) 420, 1289
MroXI GAANNNNTTC 1 cut(s) 288
MseI TTAA 4 cut(s) 194, 800, 1041, 1467
MslI CAYNNNNRTG 3 cut(s) 527, 545, 1542
MspI CCGG 5 cut(s) 220, 335, 384, 496, 1302
MspR9I CCNGG 5 cut(s) 335, 384, 666, 1036, 1510
MunI CAATTG 2 cut(s) 429, 1385
Mva1269I GAATGC 1 cut(s) 304
MvaI CCWGG 3 cut(s) 666, 1036, 1510
MvnI CGCG 1 cut(s) 1543
MwoI GCNNNNNNNGC 2 cut(s) 37, 1540
NciI CCSGG 2 cut(s) 335, 384
NlaIV GGNNCC 1 cut(s) 643
NmuCI GTSAC 3 cut(s) 614, 672, 682
NsbI TGCGCA 1 cut(s) 1166
NspI RCATGY 3 cut(s) 165, 544, 916
NspV TTCGAA 1 cut(s) 988
OliI CACNNNNGTG 1 cut(s) 1542
PaeI GCATGC 1 cut(s) 165
PasI CCCWGGG 1 cut(s) 1035
PceI AGGCCT 2 cut(s) 476, 1377
PciI ACATGT 1 cut(s) 540
PctI GAATGC 1 cut(s) 304
PdmI GAANNNNTTC 1 cut(s) 288
PfeI GAWTC 3 cut(s) 443, 1092, 1479
PfoI TCCNGGA 1 cut(s) 1508
PkrI GCNGC 2 cut(s) 518, 521
PleI GAGTC 4 cut(s) 361, 679, 1414, 1468
PpsI GAGTC 4 cut(s) 361, 679, 1414, 1468
PscI ACATGT 1 cut(s) 540
PshAI GACNNNNGTC 1 cut(s) 50
PsiI TTATAA 1 cut(s) 359
Psp6I CCWGG 3 cut(s) 664, 1034, 1508
PspFI CCCAGC 1 cut(s) 558
PspGI CCWGG 3 cut(s) 664, 1034, 1508
PspN4I GGNNCC 1 cut(s) 643
PspPI GGNCC 4 cut(s) 39, 498, 662, 1192
PsrI GAACNNNNNNTAC 2 cut(s) 743, 775
PstNI CAGNNNCTG 1 cut(s) 422
PsuI RGATCY 1 cut(s) 1011
RsaI GTAC 3 cut(s) 264, 575, 761
RsaNI GTAC 3 cut(s) 263, 574, 760
RseI CAYNNNNRTG 3 cut(s) 527, 545, 1542
SaqAI TTAA 4 cut(s) 194, 800, 1041, 1467
SatI GCNGC 2 cut(s) 517, 520
Sau96I GGNCC 4 cut(s) 39, 498, 662, 1192
SchI GAGTC 4 cut(s) 361, 680, 1415, 1469
ScrFI CCNGG 5 cut(s) 335, 384, 666, 1036, 1510
SexAI ACCWGGT 1 cut(s) 664
SfaNI GCATC 2 cut(s) 254, 1152
SfcI CTRYAG 1 cut(s) 946
SfuI TTCGAA 1 cut(s) 988
SinI GGWCC 3 cut(s) 498, 662, 1192
SmiMI CAYNNNNRTG 3 cut(s) 527, 545, 1542
SmlI CTYRAG 2 cut(s) 511, 754
SmoI CTYRAG 2 cut(s) 511, 754
SphI GCATGC 1 cut(s) 165
Sse9I AATT 9 cut(s) 13, 268, 429, 900, 906, 1114, 1285, 1385, 1561
SseBI AGGCCT 2 cut(s) 476, 1377
SsiI CCGC 3 cut(s) 493, 517, 1138
SspMI CTAG 1 cut(s) 1079
StuI AGGCCT 2 cut(s) 476, 1377
StyD4I CCNGG 5 cut(s) 333, 382, 664, 1034, 1508
TaaI ACNGT 5 cut(s) 573, 578, 690, 805, 1472
TaiI ACGT 2 cut(s) 264, 1225
TaqI TCGA 7 cut(s) 568, 589, 722, 988, 1090, 1409, 1458
TaqII GACCGA 1 cut(s) 625
TasI AATT 9 cut(s) 13, 268, 429, 900, 906, 1114, 1285, 1385, 1561
TauI GCSGC 1 cut(s) 519
TfiI GAWTC 3 cut(s) 443, 1092, 1479
Tru1I TTAA 4 cut(s) 194, 800, 1041, 1467
Tru9I TTAA 4 cut(s) 194, 800, 1041, 1467
TscAI CASTG 2 cut(s) 54, 583
TseFI GTSAC 3 cut(s) 614, 672, 682
TseI GCWGC 1 cut(s) 519
Tsp45I GTSAC 3 cut(s) 614, 672, 682
TspDTI ATGAA 6 cut(s) 24, 141, 860, 931, 996, 1071
TspGWI ACGGA 1 cut(s) 990
TspRI CASTG 2 cut(s) 54, 583
VpaK11BI GGWCC 3 cut(s) 498, 662, 1192
XapI RAATTY 2 cut(s) 13, 1561
XceI RCATGY 3 cut(s) 165, 544, 916
XcmI CCANNNNNNNNNTGG 1 cut(s) 781
XmnI GAANNNNTTC 1 cut(s) 288
XspI CTAG 1 cut(s) 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.