Rh6CG336100

Type I inositol 1,4,5-trisphosphate 5-phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0012832
Physical Location & Seq
Forward (+)
52410899 .. 52416113
5215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG336100.1

Sequence Viewer

Length: 1440 bp
ATGGACGTATCACAGGTTACAGACGTACAAAACTATAGGATTTTTGTAGCTACATGGAATGTGGCTGGAAAATCTCCTCCGAGTTGTTTGAATCTAGAAGATTGGCTTCATACCTCTCCTCCTGCTGACATTTATGTTCTAGGGTTTCAAGAAATAGTTCCTTTGAATGCTGGCAATGTGTTGGGGACAGAGGACAATGCCCCGGCTAAAAAATGGGTAGCCCTTATAAGGAAGACTCTAAATAGTCTTCCCGGCACAAGTGGTGGTTGCCATACACCTTCGCCAGTTCCTGATCCAATTGTGGAGTTGGATTCAGACTTTGAGGGATCAACAAGGCAGAAGGCCTCCTCCTTCTTCCACCGCCGGTCCTTTCAATCCTTGAGCCGCAGCATGAGAATGGAGAATGACATGTCAATGCCACAACCCCAGCTTGATCGACGGTACAGTGTTTGTGATCGAGTTATTTTTGGGAATAGACCGAGTGACTATGACCCCAATGTAAGATGGGGTTCCTCTGATGATGATAATGGACCTGGTGAGTCACCTGTTGTCACACAGTATTCACCAATGTCCAATAGTGGATCATTTTCAATAGAGGATAGAGAAAGACAGCAAGGGAACTCAAGGTACTGCTTGGTTGCCAGCAAGCAAATGGTTGGGATATTTTTAACGGTGTGGGTGAGAAGTGATCTCAGAGATGATGTTCGCAATATGAAGGTGTCTTGTGTTGGCAGAGGATTGATGGGCTATCTTGGAAATAAGGGTTCAATTTCAATTAGCATGTCCTTGCACCAAACAAGCTTCTGCTTCATCTGTAGTCATCTAACCTCTGGGCAGAAGGACGGAGATGAACTTCGAAGAAACTCTGATGTCTTGGAGATCCTTAGGAAGACAAGGTTTCCCAGGGTTAATGGAATGGGAGATGAAAGCTCTCCTCAGACTATACTAGAGCATGATCGGATCATTTGGCTCGGTGATTTGAATTATCGCATTGCCCTTTCCTACCGCTATGCAAAAGCTCTTGTTGAGATGCGCAACTGGAGGGCATTGTTAGAGAAGGACCAGCTGAGGATAGAGCAGAGACGAGGACGTGTCTTTGGGGGATGGAGTGAAGGGAAGATATATTTCCCTCCGACATACAAGTATTTGAACAATTCAGATAGGTATGCCGGTGAAGATAGGCACAACAAGGAGAAGCGAAGAACTCCAGCATGGTGTGATCGTATATTATGGCATGGAAGAGGCCTCCTTCAATTGTCCTATGTTCGTGGGGAGTCGAGGTTCTCAGATCACAGGCCAGTTTACAGCGTATTTCTGGCAGAGGTCGAGTCCATTAACCGTAACCGAATCAAGAAAAGCATGAGTTGTTCCAGTTCCAGGATTGAGGTAGAGGAGCTGTTGCCACACTCGTGTGGATACTCCCAACTAAATTTCTATTGA

Protein Analysis

479

Amino Acids

54.7

Weight (kDa)

8.8

Isoelectric Point (pI)

61.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 17 - 431 2e-06 Endonuclease/Exonuclease/phosphatase family
Exo_endo_phos2 PF22669 18 - 82 1.6e-07 Endonuclease/Exonuclease/phosphatase family 2
Exo_endo_phos2 PF22669 203 - 436 4.3e-63 Endonuclease/Exonuclease/phosphatase family 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63240 AT3G63240
fragaria_vesca FvH4_2g23170
malus_domestica MD03G1261100.v1.1 MD11G1281400.v1.1
prunus_persica Prupe.8G236900_v2.0.a1
pyrus_communis pycom03g20850 pycom11g24940
rosa_chinensis RchiOBHm_Chr6g0290421
rosa_laevigata RLG00000012220
rosa_multiflora Rmu_sc0002890.1_g000001
rosa_roxburghii Rroxscaffold_7G00177080
rosa_rugosa Rorug06G0212600
rosa_samantha Rh6AG322600 Rh6BG330100 Rh6CG336100
rosa_wichuraiana Rw6G027880 Rw6G027970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 227
Acc16I TGCGCA 1 cut(s) 1034
AciI CCGC 3 cut(s) 361, 385, 1006
AclWI GGATC 5 cut(s) 287, 334, 589, 874, 968
AcsI RAATTY 1 cut(s) 1429
AfaI GTAC 3 cut(s) 27, 443, 629
AfiI CCNNNNNNNGG 2 cut(s) 228, 1377
AflIII ACRYGT 2 cut(s) 408, 1090
AjiI CACGTC 1 cut(s) 1091
AjnI CCWGG 3 cut(s) 532, 902, 1376
AleI CACNNNNGTG 2 cut(s) 1408, 1410
AloI GAACNNNNNNTCC 2 cut(s) 1265, 1297
AluBI AGCT 7 cut(s) 50, 430, 801, 930, 1019, 1066, 1396
AluI AGCT 7 cut(s) 50, 430, 801, 930, 1019, 1066, 1396
Alw26I GTCTC 1 cut(s) 1075
AlwI GGATC 5 cut(s) 287, 334, 589, 874, 968
AlwNI CAGNNNCTG 1 cut(s) 290
AoxI GGCC 3 cut(s) 342, 1243, 1295
ApeKI GCWGC 1 cut(s) 387
ApoI RAATTY 1 cut(s) 1429
Asp700I GAANNNNTTC 1 cut(s) 156
AspLEI GCGC 1 cut(s) 1035
AspS9I GGNCC 3 cut(s) 366, 530, 1060
AsuC2I CCSGG 2 cut(s) 203, 252
AsuHPI GGTGA 6 cut(s) 534, 548, 555, 691, 986, 1184
AsuII TTCGAA 1 cut(s) 856
AvaII GGWCC 3 cut(s) 366, 530, 1060
AxyI CCTNAGG 1 cut(s) 884
BauI CACGAG 1 cut(s) 1408
BbsI GAAGAC 3 cut(s) 239, 239, 896
BbvCI CCTCAGC 1 cut(s) 1067
BbvI GCAGC 1 cut(s) 399
BccI CCATC 3 cut(s) 498, 736, 1098
BciT130I CCWGG 3 cut(s) 534, 904, 1378
BciVI GTATCC 1 cut(s) 1409
BcnI CCSGG 2 cut(s) 203, 252
BcoDI GTCTC 1 cut(s) 1075
BfaI CTAG 3 cut(s) 95, 140, 947
BfmI CTRYAG 2 cut(s) 34, 814
BfuI GTATCC 1 cut(s) 1409
BisI GCNGC 2 cut(s) 385, 388
BlsI GCNGC 2 cut(s) 386, 389
Bme1390I CCNGG 5 cut(s) 203, 252, 534, 904, 1378
Bme18I GGWCC 3 cut(s) 366, 530, 1060
BmgBI CACGTC 1 cut(s) 1091
BmgT120I GGNCC 3 cut(s) 366, 530, 1060
BmiI GGNNCC 1 cut(s) 511
BmrFI CCNGG 5 cut(s) 203, 252, 534, 904, 1378
BmsI GCATC 1 cut(s) 1020
BpiI GAAGAC 3 cut(s) 239, 239, 896
BpmI CTGGAG 2 cut(s) 1060, 1191
Bpu10I CCTNAGC 1 cut(s) 1067
Bpu14I TTCGAA 1 cut(s) 856
BpuEI CTTGAG 2 cut(s) 400, 607
BpuMI CCSGG 2 cut(s) 203, 252
BsaJI CCNNGG 3 cut(s) 201, 902, 903
BsaXI ACNNNNNCTCC 4 cut(s) 103, 133, 1265, 1295
Bsc4I CCNNNNNNNGG 2 cut(s) 228, 1377
Bse118I RCCGGY 2 cut(s) 363, 1169
Bse1I ACTGG 4 cut(s) 284, 1043, 1298, 1371
Bse21I CCTNAGG 1 cut(s) 884
Bse3DI GCAATG 2 cut(s) 181, 990
BseBI CCWGG 3 cut(s) 534, 904, 1378
BseDI CCNNGG 3 cut(s) 201, 902, 903
BseGI GGATG 1 cut(s) 1109
BseLI CCNNNNNNNGG 2 cut(s) 228, 1377
BseMI GCAATG 2 cut(s) 181, 990
BseMII CTCAG 4 cut(s) 706, 950, 1058, 1299
BseNI ACTGG 4 cut(s) 284, 1043, 1298, 1371
BseRI GAGGAG 5 cut(s) 66, 108, 337, 924, 1406
BseXI GCAGC 1 cut(s) 399
BseYI CCCAGC 1 cut(s) 426
BshFI GGCC 3 cut(s) 344, 1245, 1297
BsiSI CCGG 4 cut(s) 203, 252, 364, 1170
BslFI GGGAC 1 cut(s) 199
BslI CCNNNNNNNGG 2 cut(s) 228, 1377
BsmAI GTCTC 1 cut(s) 1075
BsmBI CGTCTC 1 cut(s) 1075
BsmFI GGGAC 1 cut(s) 199
BsmI GAATGC 1 cut(s) 172
BsnI GGCC 3 cut(s) 344, 1245, 1297
Bsp119I TTCGAA 1 cut(s) 856
BspACI CCGC 3 cut(s) 361, 385, 1006
BspANI GGCC 3 cut(s) 344, 1245, 1297
BspCNI CTCAG 4 cut(s) 705, 949, 1059, 1298
BspLI GGNNCC 1 cut(s) 511
BspPI GGATC 5 cut(s) 287, 334, 589, 874, 968
BspT104I TTCGAA 1 cut(s) 856
BsrDI GCAATG 2 cut(s) 181, 990
BsrFI RCCGGY 2 cut(s) 363, 1169
BsrI ACTGG 4 cut(s) 284, 1043, 1298, 1371
BssAI RCCGGY 2 cut(s) 363, 1169
BssECI CCNNGG 3 cut(s) 201, 902, 903
BssSI CACGAG 1 cut(s) 1408
Bst2BI CACGAG 1 cut(s) 1408
Bst2UI CCWGG 3 cut(s) 534, 904, 1378
Bst4CI ACNGT 5 cut(s) 441, 446, 558, 673, 1340
Bst6I CTCTTC 1 cut(s) 1234
BstBI TTCGAA 1 cut(s) 856
BstC8I GCNNGC 3 cut(s) 172, 643, 647
BstDEI CTNAG 5 cut(s) 692, 884, 936, 1067, 1285
BstF5I GGATG 1 cut(s) 1109
BstHHI GCGC 1 cut(s) 1035
BstMAI GTCTC 1 cut(s) 1075
BstNI CCWGG 3 cut(s) 534, 904, 1378
BstNSI RCATGY 2 cut(s) 412, 784
BstSCI CCNGG 5 cut(s) 201, 250, 532, 902, 1376
BstSFI CTRYAG 2 cut(s) 34, 814
BstV1I GCAGC 1 cut(s) 399
BstV2I GAAGAC 3 cut(s) 239, 239, 896
BstX2I RGATCY 1 cut(s) 879
BstYI RGATCY 1 cut(s) 879
Bsu36I CCTNAGG 1 cut(s) 884
BsuI GTATCC 1 cut(s) 1409
BsuRI GGCC 3 cut(s) 344, 1245, 1297
BtrI CACGTC 1 cut(s) 1091
BtsCI GGATG 1 cut(s) 1109
BtsIMutI CAGTG 1 cut(s) 451
Cac8I GCNNGC 3 cut(s) 172, 643, 647
CaiI CAGNNNCTG 1 cut(s) 290
CfoI GCGC 1 cut(s) 1035
Cfr10I RCCGGY 2 cut(s) 363, 1169
Cfr13I GGNCC 3 cut(s) 366, 530, 1060
CsiI ACCWGGT 1 cut(s) 532
Csp6I GTAC 3 cut(s) 26, 442, 628
CviAII CATG 8 cut(s) 54, 391, 409, 781, 953, 1212, 1235, 1360
CviQI GTAC 3 cut(s) 26, 442, 628
DdeI CTNAG 5 cut(s) 692, 884, 936, 1067, 1285
Eam1104I CTCTTC 1 cut(s) 1234
EarI CTCTTC 1 cut(s) 1234
Eco147I AGGCCT 2 cut(s) 344, 1245
Eco47I GGWCC 3 cut(s) 366, 530, 1060
Eco81I CCTNAGG 1 cut(s) 884
EcoRII CCWGG 3 cut(s) 532, 902, 1376
Esp3I CGTCTC 1 cut(s) 1075
FaeI CATG 8 cut(s) 57, 394, 412, 784, 956, 1215, 1238, 1363
FalI AAGNNNNNCTT 2 cut(s) 90, 122
FaqI GGGAC 1 cut(s) 199
FatI CATG 8 cut(s) 53, 390, 408, 780, 952, 1211, 1234, 1359
Fnu4HI GCNGC 2 cut(s) 385, 388
FokI GGATG 1 cut(s) 1116
Fsp4HI GCNGC 2 cut(s) 385, 388
FspBI CTAG 3 cut(s) 95, 140, 947
FspI TGCGCA 1 cut(s) 1034
GlaI GCGC 1 cut(s) 1034
GluI GCNGC 2 cut(s) 385, 388
GsaI CCCAGC 1 cut(s) 430
GsuI CTGGAG 2 cut(s) 1060, 1191
HaeIII GGCC 3 cut(s) 344, 1245, 1297
HapII CCGG 4 cut(s) 203, 252, 364, 1170
HhaI GCGC 1 cut(s) 1035
Hin1II CATG 8 cut(s) 57, 394, 412, 784, 956, 1215, 1238, 1363
Hin6I GCGC 1 cut(s) 1033
HinP1I GCGC 1 cut(s) 1033
HindIII AAGCTT 1 cut(s) 799
HinfI GANTC 7 cut(s) 91, 235, 311, 539, 1274, 1328, 1347
HpaII CCGG 4 cut(s) 203, 252, 364, 1170
HphI GGTGA 6 cut(s) 534, 548, 555, 691, 986, 1184
Hpy166II GTNNAC 1 cut(s) 1303
Hpy188III TCNNGA 4 cut(s) 95, 149, 290, 1351
Hpy8I GTNNAC 1 cut(s) 1303
Hpy99I CGWCG 1 cut(s) 441
HpyAV CCTTC 8 cut(s) 288, 334, 361, 709, 832, 1051, 1106, 1259
HpyCH4III ACNGT 5 cut(s) 441, 446, 558, 673, 1340
HpyCH4IV ACGT 3 cut(s) 6, 24, 1090
HpyCH4V TGCA 2 cut(s) 790, 1013
HpyF3I CTNAG 5 cut(s) 692, 884, 936, 1067, 1285
HpySE526I ACGT 3 cut(s) 6, 24, 1090
Hsp92II CATG 8 cut(s) 57, 394, 412, 784, 956, 1215, 1238, 1363
HspAI GCGC 1 cut(s) 1033
LmnI GCTCC 1 cut(s) 1393
Lsp1109I GCAGC 1 cut(s) 399
LweI GCATC 1 cut(s) 1020
MabI ACCWGGT 1 cut(s) 532
MaeI CTAG 3 cut(s) 95, 140, 947
MaeII ACGT 3 cut(s) 6, 24, 1090
MaeIII GTNAC 5 cut(s) 16, 482, 540, 550, 1340
MfeI CAATTG 2 cut(s) 297, 1253
MflI RGATCY 1 cut(s) 879
MluCI AATT 7 cut(s) 297, 768, 774, 982, 1153, 1253, 1429
MlyI GAGTC 4 cut(s) 229, 548, 1283, 1337
MmeI TCCRAC 2 cut(s) 288, 1157
MroXI GAANNNNTTC 1 cut(s) 156
MseI TTAA 3 cut(s) 668, 909, 1335
MslI CAYNNNNRTG 4 cut(s) 395, 413, 1408, 1410
MspA1I CMGCKG 1 cut(s) 1066
MspI CCGG 4 cut(s) 203, 252, 364, 1170
MspR9I CCNGG 5 cut(s) 203, 252, 534, 904, 1378
MunI CAATTG 2 cut(s) 297, 1253
Mva1269I GAATGC 1 cut(s) 172
MvaI CCWGG 3 cut(s) 534, 904, 1378
NciI CCSGG 2 cut(s) 203, 252
NlaIII CATG 8 cut(s) 57, 394, 412, 784, 956, 1215, 1238, 1363
NlaIV GGNNCC 1 cut(s) 511
NmuCI GTSAC 3 cut(s) 482, 540, 550
NsbI TGCGCA 1 cut(s) 1034
NspI RCATGY 2 cut(s) 412, 784
NspV TTCGAA 1 cut(s) 856
OliI CACNNNNGTG 2 cut(s) 1408, 1410
PasI CCCWGGG 1 cut(s) 903
PceI AGGCCT 2 cut(s) 344, 1245
PciI ACATGT 1 cut(s) 408
PctI GAATGC 1 cut(s) 172
PdmI GAANNNNTTC 1 cut(s) 156
PfeI GAWTC 3 cut(s) 91, 311, 1347
PfoI TCCNGGA 1 cut(s) 1376
PkrI GCNGC 2 cut(s) 386, 389
PleI GAGTC 4 cut(s) 229, 547, 1282, 1336
PpsI GAGTC 4 cut(s) 229, 547, 1282, 1336
PscI ACATGT 1 cut(s) 408
PsiI TTATAA 1 cut(s) 227
Psp6I CCWGG 3 cut(s) 532, 902, 1376
PspFI CCCAGC 1 cut(s) 426
PspGI CCWGG 3 cut(s) 532, 902, 1376
PspN4I GGNNCC 1 cut(s) 511
PspPI GGNCC 3 cut(s) 366, 530, 1060
PsrI GAACNNNNNNTAC 2 cut(s) 611, 643
PstNI CAGNNNCTG 1 cut(s) 290
PsuI RGATCY 1 cut(s) 879
PvuII CAGCTG 1 cut(s) 1066
RsaI GTAC 3 cut(s) 27, 443, 629
RsaNI GTAC 3 cut(s) 26, 442, 628
RseI CAYNNNNRTG 4 cut(s) 395, 413, 1408, 1410
SaqAI TTAA 3 cut(s) 668, 909, 1335
SatI GCNGC 2 cut(s) 385, 388
Sau96I GGNCC 3 cut(s) 366, 530, 1060
SchI GAGTC 4 cut(s) 229, 548, 1283, 1337
ScrFI CCNGG 5 cut(s) 203, 252, 534, 904, 1378
SexAI ACCWGGT 1 cut(s) 532
SfaNI GCATC 1 cut(s) 1020
SfcI CTRYAG 2 cut(s) 34, 814
SfuI TTCGAA 1 cut(s) 856
SinI GGWCC 3 cut(s) 366, 530, 1060
SmiMI CAYNNNNRTG 4 cut(s) 395, 413, 1408, 1410
SmlI CTYRAG 2 cut(s) 379, 622
SmoI CTYRAG 2 cut(s) 379, 622
Sse9I AATT 7 cut(s) 297, 768, 774, 982, 1153, 1253, 1429
SseBI AGGCCT 2 cut(s) 344, 1245
SsiI CCGC 3 cut(s) 361, 385, 1006
SspMI CTAG 3 cut(s) 95, 140, 947
StuI AGGCCT 2 cut(s) 344, 1245
StyD4I CCNGG 5 cut(s) 201, 250, 532, 902, 1376
TaaI ACNGT 5 cut(s) 441, 446, 558, 673, 1340
TaiI ACGT 3 cut(s) 9, 27, 1093
TaqI TCGA 5 cut(s) 436, 457, 856, 1277, 1326
TaqII GACCGA 1 cut(s) 493
TasI AATT 7 cut(s) 297, 768, 774, 982, 1153, 1253, 1429
TauI GCSGC 1 cut(s) 387
TfiI GAWTC 3 cut(s) 91, 311, 1347
Tru1I TTAA 3 cut(s) 668, 909, 1335
Tru9I TTAA 3 cut(s) 668, 909, 1335
TscAI CASTG 1 cut(s) 451
TseFI GTSAC 3 cut(s) 482, 540, 550
TseI GCWGC 1 cut(s) 387
Tsp45I GTSAC 3 cut(s) 482, 540, 550
TspDTI ATGAA 5 cut(s) 98, 728, 799, 864, 939
TspGWI ACGGA 1 cut(s) 858
TspRI CASTG 1 cut(s) 451
VpaK11BI GGWCC 3 cut(s) 366, 530, 1060
XapI RAATTY 1 cut(s) 1429
XbaI TCTAGA 1 cut(s) 94
XceI RCATGY 2 cut(s) 412, 784
XcmI CCANNNNNNNNNTGG 1 cut(s) 649
XmnI GAANNNNTTC 1 cut(s) 156
XspI CTAG 3 cut(s) 95, 140, 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.