RchiOBHm_Chr7g0207331

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0017729
Physical Location & Seq
Reverse (-)
24716093 .. 24716476
384 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18555

Sequence Viewer

Length: 384 bp
ATGGAGAACATCCTGATTCCTGACAATGCAAACTGCTTGCCTGAGGGGTTAGTCCGGCAAGTAAAGATGGCAACCAAGAGTGCTGGAATTGAACTTGCAGGAGAGAATGCACTGGAGAGGTATGATTCTGGTGCATATGGACAAGTTTTGGCCACAAGCAGGTCAGATTCCGGCAATGCGTTGAGTGCTTTCACATACCTAAGATTGAATGAGAGATTGTTCGAAGGGATCAACTGGCGGAACATGGTTGAATTTGTGAAAGGCATGGCTGAAGGTGGCCGGAATGAAAGACTGTCAGAGTGTGACTCCACTGGAACCTACCTTTTTGTCCGCTTTATCAAGGAGAAGAATGTCCAGGAAGAAAGGGAAACTGTTCTTGTGTAG

Protein Analysis

127

Amino Acids

14.21

Weight (kDa)

4.96

Isoelectric Point (pI)

44.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 6 - 85 1.2e-27 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 150
AciI CCGC 2 cut(s) 238, 331
AclWI GGATC 1 cut(s) 236
AcoI YGGCCR 2 cut(s) 150, 277
AcsI RAATTY 1 cut(s) 251
AcuI CTGAAG 1 cut(s) 291
AfiI CCNNNNNNNGG 1 cut(s) 159
AgsI TTSAA 3 cut(s) 92, 208, 251
AjnI CCWGG 1 cut(s) 354
AlwI GGATC 1 cut(s) 236
AoxI GGCC 2 cut(s) 150, 277
ApoI RAATTY 1 cut(s) 251
Asp700I GAANNNNTTC 1 cut(s) 372
AsuII TTCGAA 1 cut(s) 222
AxyI CCTNAGG 1 cut(s) 42
BalI TGGCCA 1 cut(s) 152
BccI CCATC 1 cut(s) 61
BciT130I CCWGG 1 cut(s) 356
BfuAI ACCTGC 1 cut(s) 150
Bme1390I CCNGG 1 cut(s) 356
BmiI GGNNCC 1 cut(s) 316
BmrFI CCNGG 1 cut(s) 356
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 1 cut(s) 134
Bpu14I TTCGAA 1 cut(s) 222
Bsc4I CCNNNNNNNGG 1 cut(s) 159
Bse1I ACTGG 3 cut(s) 117, 239, 316
Bse21I CCTNAGG 1 cut(s) 42
Bse3DI GCAATG 1 cut(s) 181
BseBI CCWGG 1 cut(s) 356
BseGI GGATG 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 159
BseMI GCAATG 1 cut(s) 181
BseMII CTCAG 1 cut(s) 33
BseNI ACTGG 3 cut(s) 117, 239, 316
BshFI GGCC 2 cut(s) 152, 279
BsiSI CCGG 3 cut(s) 55, 171, 280
BslI CCNNNNNNNGG 1 cut(s) 159
BsmI GAATGC 1 cut(s) 112
BsnI GGCC 2 cut(s) 152, 279
Bsp119I TTCGAA 1 cut(s) 222
Bsp143I GATC 1 cut(s) 228
BspACI CCGC 2 cut(s) 238, 331
BspANI GGCC 2 cut(s) 152, 279
BspCNI CTCAG 1 cut(s) 34
BspLI GGNNCC 1 cut(s) 316
BspMI ACCTGC 1 cut(s) 150
BspPI GGATC 1 cut(s) 236
BspT104I TTCGAA 1 cut(s) 222
BsrDI GCAATG 1 cut(s) 181
BsrI ACTGG 3 cut(s) 117, 239, 316
BssMI GATC 1 cut(s) 228
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 2 cut(s) 294, 373
BstBI TTCGAA 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 2 cut(s) 42, 200
BstF5I GGATG 1 cut(s) 9
BstKTI GATC 1 cut(s) 231
BstMBI GATC 1 cut(s) 228
BstMWI GCNNNNNNNGC 1 cut(s) 185
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 354
Bsu36I CCTNAGG 1 cut(s) 42
BsuRI GGCC 2 cut(s) 152, 279
BtsCI GGATG 1 cut(s) 9
BtsIMutI CAGTG 2 cut(s) 110, 309
BveI ACCTGC 1 cut(s) 150
Cac8I GCNNGC 1 cut(s) 38
CviAII CATG 2 cut(s) 244, 265
CviJI RGCY 3 cut(s) 152, 269, 279
CviKI_1 RGCY 3 cut(s) 152, 269, 279
DdeI CTNAG 2 cut(s) 42, 200
DpnI GATC 1 cut(s) 230
DpnII GATC 1 cut(s) 228
EaeI YGGCCR 2 cut(s) 150, 277
EciI GGCGGA 1 cut(s) 253
Eco57I CTGAAG 1 cut(s) 291
Eco81I CCTNAGG 1 cut(s) 42
EcoRII CCWGG 1 cut(s) 354
FaeI CATG 2 cut(s) 247, 268
FaiI YATR 6 cut(s) 123, 136, 138, 196, 245, 266
FatI CATG 2 cut(s) 243, 264
FauNDI CATATG 1 cut(s) 136
GsuI CTGGAG 1 cut(s) 134
HaeIII GGCC 2 cut(s) 152, 279
HapII CCGG 3 cut(s) 55, 171, 280
Hin1II CATG 2 cut(s) 247, 268
HinfI GANTC 4 cut(s) 16, 125, 167, 305
HpaII CCGG 3 cut(s) 55, 171, 280
Hpy188I TCNGA 2 cut(s) 166, 298
Hpy188III TCNNGA 2 cut(s) 13, 20
HpyAV CCTTC 2 cut(s) 218, 266
HpyCH4III ACNGT 2 cut(s) 294, 373
HpyCH4V TGCA 4 cut(s) 29, 98, 110, 134
HpyF10VI GCNNNNNNNGC 1 cut(s) 185
HpyF3I CTNAG 2 cut(s) 42, 200
Hsp92II CATG 2 cut(s) 247, 268
Kzo9I GATC 1 cut(s) 228
MaeIII GTNAC 1 cut(s) 302
MalI GATC 1 cut(s) 230
MboI GATC 1 cut(s) 228
MboII GAAGA 2 cut(s) 358, 371
MlsI TGGCCA 1 cut(s) 152
MluCI AATT 2 cut(s) 87, 251
MluNI TGGCCA 1 cut(s) 152
MlyI GAGTC 1 cut(s) 299
MnlI CCTC 2 cut(s) 37, 111
Mox20I TGGCCA 1 cut(s) 152
MroXI GAANNNNTTC 1 cut(s) 372
MscI TGGCCA 1 cut(s) 152
Msp20I TGGCCA 1 cut(s) 152
MspI CCGG 3 cut(s) 55, 171, 280
MspR9I CCNGG 1 cut(s) 356
Mva1269I GAATGC 1 cut(s) 112
MvaI CCWGG 1 cut(s) 356
MwoI GCNNNNNNNGC 1 cut(s) 185
NdeI CATATG 1 cut(s) 136
NdeII GATC 1 cut(s) 228
NlaIII CATG 2 cut(s) 247, 268
NlaIV GGNNCC 1 cut(s) 316
NmuCI GTSAC 1 cut(s) 302
NspV TTCGAA 1 cut(s) 222
PctI GAATGC 1 cut(s) 112
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 3 cut(s) 16, 125, 167
PfoI TCCNGGA 1 cut(s) 354
PleI GAGTC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 354
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 316
Sau3AI GATC 1 cut(s) 228
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 1 cut(s) 356
SetI ASST 6 cut(s) 122, 164, 201, 277, 320, 324
SfuI TTCGAA 1 cut(s) 222
Sse9I AATT 2 cut(s) 87, 251
SsiI CCGC 2 cut(s) 238, 331
StyD4I CCNGG 1 cut(s) 354
TaaI ACNGT 2 cut(s) 294, 373
TaqI TCGA 1 cut(s) 222
TasI AATT 2 cut(s) 87, 251
TfiI GAWTC 3 cut(s) 16, 125, 167
TscAI CASTG 2 cut(s) 117, 316
TseFI GTSAC 1 cut(s) 302
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 1 cut(s) 300
TspRI CASTG 2 cut(s) 117, 316
XapI RAATTY 1 cut(s) 251
XmnI GAANNNNTTC 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.