Rh7AG232300

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0017729
Physical Location & Seq
Reverse (-)
22408872 .. 22409261
390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG232300.1

Sequence Viewer

Length: 390 bp
ATGGAAATGAAAGATGGAGAACAGCCTGACAATGCAAATTGCTCGCCTGAGGGGTTAGTCCGGCAAGTAAAGATGGCAACCAAGAGTGTTGGAATTGAACTTGCAGGAGAGAATGCTCTGGAGAGGTATGATTCTGGTGCATATGGACAAGTTTTGGCCACAAGCAGATCAGATTTCGGCAATCCGTTGAGTGCTTTCACATACCTAAGATTGAATAAGAGATTGTTCGAAGGGGAAAACTGGCGGAACATGGTTGAATTTGTGAAAGGCATGGCTGAAGGTGGCCGGAATGAAAGACTGTCAGAGTGTGACTCCACTGGAACCAACCTTTTTGTCCGCCTTATCAAGGAGAAGAATGTCCAGGAAGAAAAGGAAACTGTTCTTGTGTAG

Protein Analysis

129

Amino Acids

14.44

Weight (kDa)

5.01

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 1 - 87 2.5e-31 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 244, 337
AcoI YGGCCR 2 cut(s) 156, 283
AcsI RAATTY 1 cut(s) 257
AcuI CTGAAG 1 cut(s) 297
AfiI CCNNNNNNNGG 1 cut(s) 346
AgsI TTSAA 3 cut(s) 98, 214, 257
AjnI CCWGG 1 cut(s) 360
AoxI GGCC 2 cut(s) 156, 283
ApoI RAATTY 1 cut(s) 257
Asp700I GAANNNNTTC 1 cut(s) 378
AsuII TTCGAA 1 cut(s) 228
AxyI CCTNAGG 1 cut(s) 48
BalI TGGCCA 1 cut(s) 158
BccI CCATC 2 cut(s) 8, 67
BcgI CGANNNNNNTGC 2 cut(s) 24, 58
BciT130I CCWGG 1 cut(s) 362
Bme1390I CCNGG 1 cut(s) 362
BmiI GGNNCC 1 cut(s) 322
BmrFI CCNGG 1 cut(s) 362
BplI GAGNNNNNCTC 2 cut(s) 296, 328
BpmI CTGGAG 1 cut(s) 140
Bpu14I TTCGAA 1 cut(s) 228
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse1I ACTGG 2 cut(s) 245, 322
Bse21I CCTNAGG 1 cut(s) 48
BseBI CCWGG 1 cut(s) 362
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMII CTCAG 1 cut(s) 39
BseNI ACTGG 2 cut(s) 245, 322
BshFI GGCC 2 cut(s) 158, 285
BsiSI CCGG 2 cut(s) 61, 286
BslI CCNNNNNNNGG 1 cut(s) 346
BsmI GAATGC 1 cut(s) 118
BsnI GGCC 2 cut(s) 158, 285
Bsp119I TTCGAA 1 cut(s) 228
Bsp143I GATC 1 cut(s) 167
BspACI CCGC 2 cut(s) 244, 337
BspANI GGCC 2 cut(s) 158, 285
BspCNI CTCAG 1 cut(s) 40
BspLI GGNNCC 1 cut(s) 322
BspT104I TTCGAA 1 cut(s) 228
BsrI ACTGG 2 cut(s) 245, 322
BssMI GATC 1 cut(s) 167
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 300, 379
BstBI TTCGAA 1 cut(s) 228
BstC8I GCNNGC 1 cut(s) 44
BstDEI CTNAG 2 cut(s) 48, 206
BstENI CCTNNNNNAGG 1 cut(s) 344
BstKTI GATC 1 cut(s) 170
BstMBI GATC 1 cut(s) 167
BstNI CCWGG 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 360
Bsu36I CCTNAGG 1 cut(s) 48
BsuRI GGCC 2 cut(s) 158, 285
BtsIMutI CAGTG 1 cut(s) 315
Cac8I GCNNGC 1 cut(s) 44
CviAII CATG 2 cut(s) 250, 271
CviJI RGCY 4 cut(s) 25, 158, 275, 285
CviKI_1 RGCY 4 cut(s) 25, 158, 275, 285
DdeI CTNAG 2 cut(s) 48, 206
DpnI GATC 1 cut(s) 169
DpnII GATC 1 cut(s) 167
EaeI YGGCCR 2 cut(s) 156, 283
EciI GGCGGA 2 cut(s) 259, 326
Eco57I CTGAAG 1 cut(s) 297
Eco81I CCTNAGG 1 cut(s) 48
EcoNI CCTNNNNNAGG 1 cut(s) 344
EcoRII CCWGG 1 cut(s) 360
FaeI CATG 2 cut(s) 253, 274
FaiI YATR 6 cut(s) 129, 142, 144, 202, 251, 272
FatI CATG 2 cut(s) 249, 270
FauNDI CATATG 1 cut(s) 142
GsuI CTGGAG 1 cut(s) 140
HaeIII GGCC 2 cut(s) 158, 285
HapII CCGG 2 cut(s) 61, 286
Hin1II CATG 2 cut(s) 253, 274
HinfI GANTC 2 cut(s) 131, 311
HpaII CCGG 2 cut(s) 61, 286
Hpy188I TCNGA 2 cut(s) 172, 304
Hpy188III TCNNGA 1 cut(s) 119
HpyAV CCTTC 2 cut(s) 224, 272
HpyCH4III ACNGT 2 cut(s) 300, 379
HpyCH4V TGCA 3 cut(s) 35, 104, 140
HpyF3I CTNAG 2 cut(s) 48, 206
Hsp92II CATG 2 cut(s) 253, 274
Kzo9I GATC 1 cut(s) 167
MaeIII GTNAC 1 cut(s) 308
MalI GATC 1 cut(s) 169
MboI GATC 1 cut(s) 167
MboII GAAGA 2 cut(s) 364, 377
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 3 cut(s) 37, 93, 257
MluNI TGGCCA 1 cut(s) 158
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 70
MnlI CCTC 2 cut(s) 43, 117
Mox20I TGGCCA 1 cut(s) 158
MroXI GAANNNNTTC 1 cut(s) 378
MscI TGGCCA 1 cut(s) 158
Msp20I TGGCCA 1 cut(s) 158
MspI CCGG 2 cut(s) 61, 286
MspR9I CCNGG 1 cut(s) 362
Mva1269I GAATGC 1 cut(s) 118
MvaI CCWGG 1 cut(s) 362
NdeI CATATG 1 cut(s) 142
NdeII GATC 1 cut(s) 167
NlaIII CATG 2 cut(s) 253, 274
NlaIV GGNNCC 1 cut(s) 322
NmuCI GTSAC 1 cut(s) 308
NspV TTCGAA 1 cut(s) 228
PctI GAATGC 1 cut(s) 118
PdmI GAANNNNTTC 1 cut(s) 378
PfeI GAWTC 1 cut(s) 131
PfoI TCCNGGA 1 cut(s) 360
PleI GAGTC 1 cut(s) 305
PpsI GAGTC 1 cut(s) 305
Psp6I CCWGG 1 cut(s) 360
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 1 cut(s) 322
Sau3AI GATC 1 cut(s) 167
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 1 cut(s) 362
SetI ASST 4 cut(s) 128, 207, 283, 330
SfuI TTCGAA 1 cut(s) 228
Sse9I AATT 3 cut(s) 37, 93, 257
SsiI CCGC 2 cut(s) 244, 337
StyD4I CCNGG 1 cut(s) 360
TaaI ACNGT 2 cut(s) 300, 379
TaqI TCGA 1 cut(s) 228
TasI AATT 3 cut(s) 37, 93, 257
TfiI GAWTC 1 cut(s) 131
TscAI CASTG 1 cut(s) 322
TseFI GTSAC 1 cut(s) 308
Tsp45I GTSAC 1 cut(s) 308
TspDTI ATGAA 2 cut(s) 23, 306
TspGWI ACGGA 1 cut(s) 174
TspRI CASTG 1 cut(s) 322
XagI CCTNNNNNAGG 1 cut(s) 344
XapI RAATTY 1 cut(s) 257
XmnI GAANNNNTTC 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.