RchiOBHm_Chr7g0207681

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0017729
Physical Location & Seq
Reverse (-)
24937882 .. 24938759
878 bp
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UTR
Exon/CDS
Intron
PRQ18585

Sequence Viewer

Length: 429 bp
ATGTTCAGCAAACATGGGGTTGTATTAAACTTCACCTGCATGGAAATGAAAGATGGAGAACAGCCTGACAATGCAAATTGCTCGCCTGAGGGGTTAGTCCGGCAAGTAAAGATGGCAACCAAGAGTGTTGGAATTGAACTTGCAGGAGAGAATGCTCTGGAGAGGTATGATTCTGGTGCATATGGACAAGTTTTGGCCACAAGCAGATCAGATTTCGGCAATCCGTTGAGTGCTTTCACATACCTAAGATTGAATAAGAGATTGTTCGAAGGGGACAACTGGCGGAACATGGTTGAATTTGTGAAAGGCATGGCTGAAGGTGGCCGGAATGAAAGACTGTCAGAGTGTGACTCCACTGGAACCAACCTTTTTGTCCGCCTTATCAAGGAGAAGAATGTCCAGGAAGAAAAGGAAACTGTTCTTGTGTAG

Protein Analysis

142

Amino Acids

15.89

Weight (kDa)

5.3

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 1 - 100 1.5e-39 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 44
AciI CCGC 2 cut(s) 283, 376
AcoI YGGCCR 2 cut(s) 195, 322
AcsI RAATTY 1 cut(s) 296
AcuI CTGAAG 1 cut(s) 336
AfiI CCNNNNNNNGG 1 cut(s) 385
AgsI TTSAA 3 cut(s) 137, 253, 296
AjnI CCWGG 1 cut(s) 399
AoxI GGCC 2 cut(s) 195, 322
ApoI RAATTY 1 cut(s) 296
Asp700I GAANNNNTTC 1 cut(s) 417
AsuHPI GGTGA 1 cut(s) 25
AsuII TTCGAA 1 cut(s) 267
AxyI CCTNAGG 1 cut(s) 87
BalI TGGCCA 1 cut(s) 197
BccI CCATC 2 cut(s) 47, 106
BcgI CGANNNNNNTGC 2 cut(s) 63, 97
BciT130I CCWGG 1 cut(s) 401
BfuAI ACCTGC 1 cut(s) 44
Bme1390I CCNGG 1 cut(s) 401
BmiI GGNNCC 1 cut(s) 361
BmrFI CCNGG 1 cut(s) 401
BplI GAGNNNNNCTC 2 cut(s) 335, 367
BpmI CTGGAG 1 cut(s) 179
Bpu14I TTCGAA 1 cut(s) 267
Bsc4I CCNNNNNNNGG 1 cut(s) 385
Bse1I ACTGG 2 cut(s) 284, 361
Bse21I CCTNAGG 1 cut(s) 87
BseBI CCWGG 1 cut(s) 401
BseLI CCNNNNNNNGG 1 cut(s) 385
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 2 cut(s) 284, 361
BshFI GGCC 2 cut(s) 197, 324
BsiSI CCGG 2 cut(s) 100, 325
BslFI GGGAC 1 cut(s) 287
BslI CCNNNNNNNGG 1 cut(s) 385
BsmFI GGGAC 1 cut(s) 287
BsmI GAATGC 1 cut(s) 157
BsnI GGCC 2 cut(s) 197, 324
Bsp119I TTCGAA 1 cut(s) 267
Bsp143I GATC 1 cut(s) 206
BspACI CCGC 2 cut(s) 283, 376
BspANI GGCC 2 cut(s) 197, 324
BspCNI CTCAG 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 361
BspMI ACCTGC 1 cut(s) 44
BspT104I TTCGAA 1 cut(s) 267
BsrI ACTGG 2 cut(s) 284, 361
BssMI GATC 1 cut(s) 206
Bst2UI CCWGG 1 cut(s) 401
Bst4CI ACNGT 2 cut(s) 339, 418
BstBI TTCGAA 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 83
BstDEI CTNAG 2 cut(s) 87, 245
BstENI CCTNNNNNAGG 1 cut(s) 383
BstKTI GATC 1 cut(s) 209
BstMBI GATC 1 cut(s) 206
BstNI CCWGG 1 cut(s) 401
BstSCI CCNGG 1 cut(s) 399
Bsu36I CCTNAGG 1 cut(s) 87
BsuRI GGCC 2 cut(s) 197, 324
BtsIMutI CAGTG 1 cut(s) 354
BveI ACCTGC 1 cut(s) 44
Cac8I GCNNGC 1 cut(s) 83
CviAII CATG 4 cut(s) 14, 40, 289, 310
CviJI RGCY 4 cut(s) 64, 197, 314, 324
CviKI_1 RGCY 4 cut(s) 64, 197, 314, 324
DdeI CTNAG 2 cut(s) 87, 245
DpnI GATC 1 cut(s) 208
DpnII GATC 1 cut(s) 206
EaeI YGGCCR 2 cut(s) 195, 322
EciI GGCGGA 2 cut(s) 298, 365
Eco57I CTGAAG 1 cut(s) 336
Eco81I CCTNAGG 1 cut(s) 87
EcoNI CCTNNNNNAGG 1 cut(s) 383
EcoRII CCWGG 1 cut(s) 399
FaeI CATG 4 cut(s) 17, 43, 292, 313
FaiI YATR 8 cut(s) 15, 41, 168, 181, 183, 241, 290, 311
FaqI GGGAC 1 cut(s) 287
FatI CATG 4 cut(s) 13, 39, 288, 309
FauNDI CATATG 1 cut(s) 181
GsuI CTGGAG 1 cut(s) 179
HaeIII GGCC 2 cut(s) 197, 324
HapII CCGG 2 cut(s) 100, 325
Hin1II CATG 4 cut(s) 17, 43, 292, 313
HinfI GANTC 2 cut(s) 170, 350
HpaII CCGG 2 cut(s) 100, 325
HphI GGTGA 1 cut(s) 25
Hpy188I TCNGA 2 cut(s) 211, 343
Hpy188III TCNNGA 1 cut(s) 158
HpyAV CCTTC 2 cut(s) 263, 311
HpyCH4III ACNGT 2 cut(s) 339, 418
HpyCH4V TGCA 4 cut(s) 39, 74, 143, 179
HpyF3I CTNAG 2 cut(s) 87, 245
Hsp92II CATG 4 cut(s) 17, 43, 292, 313
Kzo9I GATC 1 cut(s) 206
MaeIII GTNAC 1 cut(s) 347
MalI GATC 1 cut(s) 208
MboI GATC 1 cut(s) 206
MboII GAAGA 2 cut(s) 403, 416
MlsI TGGCCA 1 cut(s) 197
MluCI AATT 3 cut(s) 76, 132, 296
MluNI TGGCCA 1 cut(s) 197
MlyI GAGTC 1 cut(s) 344
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 2 cut(s) 82, 156
Mox20I TGGCCA 1 cut(s) 197
MroXI GAANNNNTTC 1 cut(s) 417
MscI TGGCCA 1 cut(s) 197
MseI TTAA 1 cut(s) 26
MslI CAYNNNNRTG 2 cut(s) 38, 44
Msp20I TGGCCA 1 cut(s) 197
MspI CCGG 2 cut(s) 100, 325
MspR9I CCNGG 1 cut(s) 401
Mva1269I GAATGC 1 cut(s) 157
MvaI CCWGG 1 cut(s) 401
NdeI CATATG 1 cut(s) 181
NdeII GATC 1 cut(s) 206
NlaIII CATG 4 cut(s) 17, 43, 292, 313
NlaIV GGNNCC 1 cut(s) 361
NmuCI GTSAC 1 cut(s) 347
NspV TTCGAA 1 cut(s) 267
PaqCI CACCTGC 1 cut(s) 44
PctI GAATGC 1 cut(s) 157
PdmI GAANNNNTTC 1 cut(s) 417
PfeI GAWTC 1 cut(s) 170
PfoI TCCNGGA 1 cut(s) 399
PleI GAGTC 1 cut(s) 344
PpsI GAGTC 1 cut(s) 344
Psp6I CCWGG 1 cut(s) 399
PspGI CCWGG 1 cut(s) 399
PspN4I GGNNCC 1 cut(s) 361
RseI CAYNNNNRTG 2 cut(s) 38, 44
SaqAI TTAA 1 cut(s) 26
Sau3AI GATC 1 cut(s) 206
SchI GAGTC 1 cut(s) 344
ScrFI CCNGG 1 cut(s) 401
SetI ASST 5 cut(s) 38, 167, 246, 322, 369
SfuI TTCGAA 1 cut(s) 267
SmiMI CAYNNNNRTG 2 cut(s) 38, 44
Sse9I AATT 3 cut(s) 76, 132, 296
SsiI CCGC 2 cut(s) 283, 376
StyD4I CCNGG 1 cut(s) 399
TaaI ACNGT 2 cut(s) 339, 418
TaqI TCGA 1 cut(s) 267
TasI AATT 3 cut(s) 76, 132, 296
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 1 cut(s) 26
Tru9I TTAA 1 cut(s) 26
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 1 cut(s) 347
Tsp45I GTSAC 1 cut(s) 347
TspDTI ATGAA 2 cut(s) 62, 345
TspGWI ACGGA 1 cut(s) 213
TspRI CASTG 1 cut(s) 361
XagI CCTNNNNNAGG 1 cut(s) 383
XapI RAATTY 1 cut(s) 296
XmnI GAANNNNTTC 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.