RCHIOBHM_MTG0498221

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Mt
OG0017099
Physical Location & Seq
Reverse (-)
36154 .. 36847
694 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15598

Sequence Viewer

Length: 672 bp
ATGGTAACAGCTGGCGTTTTTATGATAGCAAGGTGCTCCCCTTTATTTGAATACCCACCTACGGCTTTGATTGTTATTACTTCTGCAGGAGCTACGACGTCATTCCTTGCGGCAACCACTGGAATATTACAGAACGATCTAAAGAGGGTCATAGCTTATTCAACTTGCAGTCAATTAGGCTATATGATCTTTGCTTGCGGCATCTCTAACTATTCGGTTAGCGTCTTTCACTTAATGAATCACGCGTTTTTCAAAGCATTACTATTCCTGAGTGCAGGTTCGGTGATTCATGCCATGTCGGATGAGCAAGATATGCGGAAGATGGGAGGGCTTGCCTCCTCGTTCCCTTTTACCTATGCCATGATGCTCATGGGCAGCTTATCTCTAATTGGATTCCCTTTTCTAACTGGATTTTATTCCAAAGATGTGATCTTAGAGCTCGCTTACACTAAGTATACCATCAGTGGGAACTTTGCTTTCTGGTTGGGAAGTGTCTCTGTCCTTTTCACTTCTTATTACTCCTTTCGTTTACTTTTTCTAACATTTCTAGTACCAACAAATTCATTCGGGCGAGACATCTTACGATGTCATGATGCGCCCATTCCTATGGCCATTCCTTCAATACTTCTGGCTCTCGGGAGTCTCTTTGTAGGATACTTGGCCAAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko05012 map00190 map01100 map04714 map04723 map05012
Pfam Domains
Protein Families
NADH5_C Proton_antipo_M Proton_antipo_N

Protein Analysis

223

Amino Acids

24.39

Weight (kDa)

8.67

Isoelectric Point (pI)

35.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 1 - 161 3e-40 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017099)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00513
fragaria_vesca FvH4_6g34420 FvH4_c1g00470
malus_domestica MD13G1126900.v1.1
pyrus_communis pycom12401g00270 pycom17g12740
rosa_chinensis RchiOBHm_MTg0498221
rosa_laevigata RLG00000000086 RLG00000000118 RLG00000000360 RLG00000000372 RLG00000000388
rosa_wichuraiana Rw0G023320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 101
Acc36I ACCTGC 1 cut(s) 266
AccI GTMKAC 1 cut(s) 455
AccII CGCG 1 cut(s) 245
AciI CCGC 3 cut(s) 110, 198, 316
AcoI YGGCCR 2 cut(s) 609, 660
AcsI RAATTY 1 cut(s) 559
AcyI GRCGYC 1 cut(s) 98
AfaI GTAC 1 cut(s) 552
AfiI CCNNNNNNNGG 2 cut(s) 61, 465
AflIII ACRYGT 1 cut(s) 243
AgsI TTSAA 4 cut(s) 50, 162, 253, 621
AluBI AGCT 5 cut(s) 11, 92, 155, 378, 439
AluI AGCT 5 cut(s) 11, 92, 155, 378, 439
Alw21I GWGCWC 2 cut(s) 38, 441
Alw26I GTCTC 3 cut(s) 499, 567, 647
Ama87I CYCGRG 1 cut(s) 635
AoxI GGCC 2 cut(s) 609, 660
ApeKI GCWGC 1 cut(s) 375
ApoI RAATTY 1 cut(s) 559
AspLEI GCGC 1 cut(s) 598
AsuHPI GGTGA 1 cut(s) 295
AvaI CYCGRG 1 cut(s) 635
BalI TGGCCA 2 cut(s) 611, 662
BanII GRGCYC 1 cut(s) 441
Bbv12I GWGCWC 2 cut(s) 38, 441
BbvI GCAGC 1 cut(s) 387
BccI CCATC 2 cut(s) 316, 467
BceAI ACGGC 1 cut(s) 78
BciVI GTATCC 1 cut(s) 647
BcoDI GTCTC 3 cut(s) 499, 567, 647
BfaI CTAG 1 cut(s) 548
BfmI CTRYAG 1 cut(s) 84
BfuAI ACCTGC 1 cut(s) 266
BfuI GTATCC 1 cut(s) 647
BisI GCNGC 3 cut(s) 111, 199, 376
BlsI GCNGC 3 cut(s) 112, 200, 377
BmeT110I CYCGRG 1 cut(s) 635
BmsI GCATC 3 cut(s) 210, 354, 583
BsaHI GRCGYC 1 cut(s) 98
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 465
Bse1I ACTGG 2 cut(s) 124, 412
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 2 cut(s) 61, 465
BseMII CTCAG 1 cut(s) 260
BseNI ACTGG 2 cut(s) 124, 412
BseRI GAGGAG 1 cut(s) 328
BseXI GCAGC 1 cut(s) 387
BsgI GTGCAG 1 cut(s) 294
Bsh1236I CGCG 1 cut(s) 245
BshFI GGCC 2 cut(s) 611, 662
BsiHKAI GWGCWC 2 cut(s) 38, 441
BsiHKCI CYCGRG 1 cut(s) 635
BslI CCNNNNNNNGG 2 cut(s) 61, 465
BsmAI GTCTC 3 cut(s) 499, 567, 647
BsnI GGCC 2 cut(s) 611, 662
BsoBI CYCGRG 1 cut(s) 635
Bsp1286I GDGCHC 2 cut(s) 38, 441
Bsp143I GATC 3 cut(s) 136, 186, 429
BspACI CCGC 3 cut(s) 110, 198, 316
BspANI GGCC 2 cut(s) 611, 662
BspCNI CTCAG 1 cut(s) 261
BspFNI CGCG 1 cut(s) 245
BspHI TCATGA 1 cut(s) 589
BspMAI CTGCAG 1 cut(s) 88
BspMI ACCTGC 1 cut(s) 266
BsrI ACTGG 2 cut(s) 124, 412
BssMI GATC 3 cut(s) 136, 186, 429
BssNAI GTATAC 1 cut(s) 456
BssNI GRCGYC 1 cut(s) 98
Bst1107I GTATAC 1 cut(s) 456
BstACI GRCGYC 1 cut(s) 98
BstAPI GCANNNNNTGC 1 cut(s) 313
BstC8I GCNNGC 4 cut(s) 13, 196, 333, 441
BstDEI CTNAG 3 cut(s) 269, 433, 450
BstF5I GGATG 1 cut(s) 307
BstFNI CGCG 1 cut(s) 245
BstHHI GCGC 1 cut(s) 598
BstKTI GATC 3 cut(s) 139, 189, 432
BstMAI GTCTC 3 cut(s) 499, 567, 647
BstMBI GATC 3 cut(s) 136, 186, 429
BstMWI GCNNNNNNNGC 1 cut(s) 313
BstSFI CTRYAG 1 cut(s) 84
BstUI CGCG 1 cut(s) 245
BstV1I GCAGC 1 cut(s) 387
BstXI CCANNNNNNTGG 1 cut(s) 607
BstZ17I GTATAC 1 cut(s) 456
BsuI GTATCC 1 cut(s) 647
BsuRI GGCC 2 cut(s) 611, 662
BtsCI GGATG 1 cut(s) 307
BtsIMutI CAGTG 2 cut(s) 117, 469
BveI ACCTGC 1 cut(s) 266
Cac8I GCNNGC 4 cut(s) 13, 196, 333, 441
CciI TCATGA 1 cut(s) 589
CfoI GCGC 1 cut(s) 598
CseI GACGC 1 cut(s) 211
Csp6I GTAC 1 cut(s) 551
CviAII CATG 5 cut(s) 290, 295, 361, 370, 590
CviQI GTAC 1 cut(s) 551
DdeI CTNAG 3 cut(s) 269, 433, 450
DpnI GATC 3 cut(s) 138, 188, 431
DpnII GATC 3 cut(s) 136, 186, 429
EaeI YGGCCR 2 cut(s) 609, 660
Ecl136II GAGCTC 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 441
Eco53kI GAGCTC 1 cut(s) 439
Eco88I CYCGRG 1 cut(s) 635
EcoICRI GAGCTC 1 cut(s) 439
EcoT38I GRGCYC 1 cut(s) 441
FaeI CATG 5 cut(s) 293, 298, 364, 373, 593
FatI CATG 5 cut(s) 289, 294, 360, 369, 589
FblI GTMKAC 1 cut(s) 455
Fnu4HI GCNGC 3 cut(s) 111, 199, 376
FokI GGATG 1 cut(s) 314
FriOI GRGCYC 1 cut(s) 441
Fsp4HI GCNGC 3 cut(s) 111, 199, 376
FspBI CTAG 1 cut(s) 548
GlaI GCGC 1 cut(s) 597
GluI GCNGC 3 cut(s) 111, 199, 376
HaeIII GGCC 2 cut(s) 611, 662
HgaI GACGC 1 cut(s) 211
HhaI GCGC 1 cut(s) 598
Hin1I GRCGYC 1 cut(s) 98
Hin1II CATG 5 cut(s) 293, 298, 364, 373, 593
Hin6I GCGC 1 cut(s) 596
HinP1I GCGC 1 cut(s) 596
HinfI GANTC 4 cut(s) 238, 286, 393, 640
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 2 cut(s) 456, 530
Hpy188I TCNGA 1 cut(s) 301
Hpy188III TCNNGA 3 cut(s) 268, 590, 637
Hpy8I GTNNAC 2 cut(s) 456, 530
Hpy99I CGWCG 1 cut(s) 100
HpyAV CCTTC 1 cut(s) 627
HpyCH4IV ACGT 1 cut(s) 98
HpyCH4V TGCA 3 cut(s) 86, 168, 275
HpyF10VI GCNNNNNNNGC 1 cut(s) 313
HpyF3I CTNAG 3 cut(s) 269, 433, 450
HpySE526I ACGT 1 cut(s) 98
Hsp92I GRCGYC 1 cut(s) 98
Hsp92II CATG 5 cut(s) 293, 298, 364, 373, 593
HspAI GCGC 1 cut(s) 596
Kzo9I GATC 3 cut(s) 136, 186, 429
LmnI GCTCC 2 cut(s) 41, 89
LpnPI CCDG 7 cut(s) 72, 105, 261, 281, 393, 466, 614
Lsp1109I GCAGC 1 cut(s) 387
LweI GCATC 3 cut(s) 210, 354, 583
MaeI CTAG 1 cut(s) 548
MaeII ACGT 1 cut(s) 98
MaeIII GTNAC 1 cut(s) 4
MalI GATC 3 cut(s) 138, 188, 431
MboI GATC 3 cut(s) 136, 186, 429
MboII GAAGA 1 cut(s) 331
MhlI GDGCHC 2 cut(s) 38, 441
MlsI TGGCCA 2 cut(s) 611, 662
MluCI AATT 3 cut(s) 173, 387, 559
MluI ACGCGT 1 cut(s) 243
MluNI TGGCCA 2 cut(s) 611, 662
MlyI GAGTC 1 cut(s) 649
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 4 cut(s) 138, 320, 346, 349
Mox20I TGGCCA 2 cut(s) 611, 662
MscI TGGCCA 2 cut(s) 611, 662
MseI TTAA 1 cut(s) 233
MslI CAYNNNNRTG 1 cut(s) 605
Msp20I TGGCCA 2 cut(s) 611, 662
MspA1I CMGCKG 1 cut(s) 11
MvnI CGCG 1 cut(s) 245
MwoI GCNNNNNNNGC 1 cut(s) 313
NdeII GATC 3 cut(s) 136, 186, 429
NlaIII CATG 5 cut(s) 293, 298, 364, 373, 593
PagI TCATGA 1 cut(s) 589
PfeI GAWTC 3 cut(s) 238, 286, 393
PkrI GCNGC 3 cut(s) 112, 200, 377
PleI GAGTC 1 cut(s) 648
PpsI GAGTC 1 cut(s) 648
Psp124BI GAGCTC 1 cut(s) 441
PstI CTGCAG 1 cut(s) 88
PvuII CAGCTG 1 cut(s) 11
RsaI GTAC 1 cut(s) 552
RsaNI GTAC 1 cut(s) 551
RseI CAYNNNNRTG 1 cut(s) 605
SacI GAGCTC 1 cut(s) 441
SaqAI TTAA 1 cut(s) 233
SatI GCNGC 3 cut(s) 111, 199, 376
Sau3AI GATC 3 cut(s) 136, 186, 429
SchI GAGTC 1 cut(s) 649
SduI GDGCHC 2 cut(s) 38, 441
SfaNI GCATC 3 cut(s) 210, 354, 583
SfcI CTRYAG 1 cut(s) 84
SmiMI CAYNNNNRTG 1 cut(s) 605
Sse9I AATT 3 cut(s) 173, 387, 559
SsiI CCGC 3 cut(s) 110, 198, 316
SspI AATATT 1 cut(s) 126
SspMI CTAG 1 cut(s) 548
SstI GAGCTC 1 cut(s) 441
TaiI ACGT 1 cut(s) 101
TasI AATT 3 cut(s) 173, 387, 559
TauI GCSGC 2 cut(s) 113, 201
TfiI GAWTC 3 cut(s) 238, 286, 393
Tru1I TTAA 1 cut(s) 233
Tru9I TTAA 1 cut(s) 233
TscAI CASTG 2 cut(s) 124, 469
TseI GCWGC 1 cut(s) 375
TspDTI ATGAA 3 cut(s) 251, 278, 552
TspRI CASTG 2 cut(s) 124, 469
XapI RAATTY 1 cut(s) 559
XcmI CCANNNNNNNNNTGG 1 cut(s) 367
XmiI GTMKAC 1 cut(s) 455
XspI CTAG 1 cut(s) 548
ZraI GACGTC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.