RLG00000000086

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Unknown
OG0017099
Physical Location & Seq
Reverse (-)
456720 .. 457097
378 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000086

Sequence Viewer

Length: 378 bp
ATGTCGGATGAGCAAGATATGCGGAAGATGGGAGGGCTTGCCTCCTCGTTCCCTTTTACCTATGCCATGATGCTCATGGGCAGCTTATCTCTAATTGGATTCCCTTTTCTAACTGGATTTTATTCCAAAGATGTGATCTTAGAGCTCGCTTACACTAAGTATACCATCAGTGGGAACTTTGCTTTCTGGTTGGGAAGTGTCTCTGTCCTTTTCACTTCTTATTACTCCTTTCGTTTACTTTTTCTAACATTTCTAGTACCAACAAATTCATTCGGGCGAGACATCTTACGATGTCATGATGCGCCCATTCCTATGGCCATTCCTTCAATACTTCTGGCTCTCGGGAGTCTCTTTGTAGGATACTTGGCCAAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko05012 map00190 map01100 map04714 map04723 map05012
Pfam Domains
Protein Families
Proton_antipo_M Proton_antipo_N

Protein Analysis

126

Amino Acids

13.93

Weight (kDa)

7.84

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 2 - 63 6.1e-10 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017099)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00513
fragaria_vesca FvH4_6g34420 FvH4_c1g00470
malus_domestica MD13G1126900.v1.1
pyrus_communis pycom12401g00270 pycom17g12740
rosa_chinensis RchiOBHm_MTg0498221
rosa_laevigata RLG00000000086 RLG00000000118 RLG00000000360 RLG00000000372 RLG00000000388
rosa_wichuraiana Rw0G023320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 161
AciI CCGC 1 cut(s) 22
AcoI YGGCCR 2 cut(s) 315, 366
AcsI RAATTY 1 cut(s) 265
AfaI GTAC 1 cut(s) 258
AfiI CCNNNNNNNGG 1 cut(s) 171
AgsI TTSAA 1 cut(s) 327
AluBI AGCT 2 cut(s) 84, 145
AluI AGCT 2 cut(s) 84, 145
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 3 cut(s) 205, 273, 353
Ama87I CYCGRG 1 cut(s) 341
AoxI GGCC 2 cut(s) 315, 366
ApeKI GCWGC 1 cut(s) 81
ApoI RAATTY 1 cut(s) 265
AspLEI GCGC 1 cut(s) 304
AvaI CYCGRG 1 cut(s) 341
BalI TGGCCA 2 cut(s) 317, 368
BanII GRGCYC 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 1 cut(s) 93
BccI CCATC 2 cut(s) 22, 173
BciVI GTATCC 1 cut(s) 353
BcoDI GTCTC 3 cut(s) 205, 273, 353
BfaI CTAG 1 cut(s) 254
BfuI GTATCC 1 cut(s) 353
BisI GCNGC 1 cut(s) 82
BlsI GCNGC 1 cut(s) 83
BmeT110I CYCGRG 1 cut(s) 341
BmsI GCATC 2 cut(s) 60, 289
Bsc4I CCNNNNNNNGG 1 cut(s) 171
Bse1I ACTGG 1 cut(s) 118
BseGI GGATG 1 cut(s) 13
BseLI CCNNNNNNNGG 1 cut(s) 171
BseNI ACTGG 1 cut(s) 118
BseRI GAGGAG 1 cut(s) 34
BseXI GCAGC 1 cut(s) 93
BshFI GGCC 2 cut(s) 317, 368
BsiHKAI GWGCWC 1 cut(s) 147
BsiHKCI CYCGRG 1 cut(s) 341
BslI CCNNNNNNNGG 1 cut(s) 171
BsmAI GTCTC 3 cut(s) 205, 273, 353
BsnI GGCC 2 cut(s) 317, 368
BsoBI CYCGRG 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 147
Bsp143I GATC 1 cut(s) 135
BspACI CCGC 1 cut(s) 22
BspANI GGCC 2 cut(s) 317, 368
BspHI TCATGA 1 cut(s) 295
BsrI ACTGG 1 cut(s) 118
BssMI GATC 1 cut(s) 135
BssNAI GTATAC 1 cut(s) 162
Bst1107I GTATAC 1 cut(s) 162
BstAPI GCANNNNNTGC 1 cut(s) 19
BstC8I GCNNGC 2 cut(s) 39, 147
BstDEI CTNAG 2 cut(s) 139, 156
BstF5I GGATG 1 cut(s) 13
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 1 cut(s) 138
BstMAI GTCTC 3 cut(s) 205, 273, 353
BstMBI GATC 1 cut(s) 135
BstMWI GCNNNNNNNGC 1 cut(s) 19
BstV1I GCAGC 1 cut(s) 93
BstXI CCANNNNNNTGG 1 cut(s) 313
BstZ17I GTATAC 1 cut(s) 162
BsuI GTATCC 1 cut(s) 353
BsuRI GGCC 2 cut(s) 317, 368
BtsCI GGATG 1 cut(s) 13
BtsIMutI CAGTG 1 cut(s) 175
Cac8I GCNNGC 2 cut(s) 39, 147
CciI TCATGA 1 cut(s) 295
CfoI GCGC 1 cut(s) 304
Csp6I GTAC 1 cut(s) 257
CviAII CATG 3 cut(s) 67, 76, 296
CviJI RGCY 6 cut(s) 37, 84, 145, 317, 338, 368
CviKI_1 RGCY 6 cut(s) 37, 84, 145, 317, 338, 368
CviQI GTAC 1 cut(s) 257
DdeI CTNAG 2 cut(s) 139, 156
DpnI GATC 1 cut(s) 137
DpnII GATC 1 cut(s) 135
EaeI YGGCCR 2 cut(s) 315, 366
Ecl136II GAGCTC 1 cut(s) 145
Eco24I GRGCYC 1 cut(s) 147
Eco53kI GAGCTC 1 cut(s) 145
Eco88I CYCGRG 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 145
EcoT38I GRGCYC 1 cut(s) 147
FaeI CATG 3 cut(s) 70, 79, 299
FaiI YATR 7 cut(s) 20, 63, 68, 77, 162, 297, 314
FatI CATG 3 cut(s) 66, 75, 295
FblI GTMKAC 1 cut(s) 161
Fnu4HI GCNGC 1 cut(s) 82
FokI GGATG 1 cut(s) 20
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 1 cut(s) 82
FspBI CTAG 1 cut(s) 254
GlaI GCGC 1 cut(s) 303
GluI GCNGC 1 cut(s) 82
HaeIII GGCC 2 cut(s) 317, 368
HhaI GCGC 1 cut(s) 304
Hin1II CATG 3 cut(s) 70, 79, 299
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HinfI GANTC 2 cut(s) 99, 346
Hpy166II GTNNAC 2 cut(s) 162, 236
Hpy188I TCNGA 1 cut(s) 7
Hpy188III TCNNGA 2 cut(s) 296, 343
Hpy8I GTNNAC 2 cut(s) 162, 236
HpyAV CCTTC 1 cut(s) 333
HpyF10VI GCNNNNNNNGC 1 cut(s) 19
HpyF3I CTNAG 2 cut(s) 139, 156
Hsp92II CATG 3 cut(s) 70, 79, 299
HspAI GCGC 1 cut(s) 302
Kzo9I GATC 1 cut(s) 135
LpnPI CCDG 3 cut(s) 99, 172, 320
Lsp1109I GCAGC 1 cut(s) 93
LweI GCATC 2 cut(s) 60, 289
MaeI CTAG 1 cut(s) 254
MalI GATC 1 cut(s) 137
MboI GATC 1 cut(s) 135
MboII GAAGA 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 147
MlsI TGGCCA 2 cut(s) 317, 368
MluCI AATT 2 cut(s) 93, 265
MluNI TGGCCA 2 cut(s) 317, 368
MlyI GAGTC 1 cut(s) 355
MnlI CCTC 3 cut(s) 26, 52, 55
Mox20I TGGCCA 2 cut(s) 317, 368
MscI TGGCCA 2 cut(s) 317, 368
MslI CAYNNNNRTG 1 cut(s) 311
Msp20I TGGCCA 2 cut(s) 317, 368
MwoI GCNNNNNNNGC 1 cut(s) 19
NdeII GATC 1 cut(s) 135
NlaIII CATG 3 cut(s) 70, 79, 299
PagI TCATGA 1 cut(s) 295
PfeI GAWTC 1 cut(s) 99
PkrI GCNGC 1 cut(s) 83
PleI GAGTC 1 cut(s) 354
PpsI GAGTC 1 cut(s) 354
Psp124BI GAGCTC 1 cut(s) 147
RsaI GTAC 1 cut(s) 258
RsaNI GTAC 1 cut(s) 257
RseI CAYNNNNRTG 1 cut(s) 311
SacI GAGCTC 1 cut(s) 147
SatI GCNGC 1 cut(s) 82
Sau3AI GATC 1 cut(s) 135
SchI GAGTC 1 cut(s) 355
SduI GDGCHC 1 cut(s) 147
SetI ASST 3 cut(s) 62, 86, 147
SfaNI GCATC 2 cut(s) 60, 289
SmiMI CAYNNNNRTG 1 cut(s) 311
Sse9I AATT 2 cut(s) 93, 265
SsiI CCGC 1 cut(s) 22
SspMI CTAG 1 cut(s) 254
SstI GAGCTC 1 cut(s) 147
TasI AATT 2 cut(s) 93, 265
TfiI GAWTC 1 cut(s) 99
TscAI CASTG 1 cut(s) 175
TseI GCWGC 1 cut(s) 81
TspDTI ATGAA 1 cut(s) 258
TspRI CASTG 1 cut(s) 175
XapI RAATTY 1 cut(s) 265
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XmiI GTMKAC 1 cut(s) 161
XspI CTAG 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.