RLG00000000388

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017099
Physical Location & Seq
Reverse (-)
795626 .. 797650
2025 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000388

Sequence Viewer

Length: 873 bp
ATGAATTGCCGAGTAGATAGATCAGATCCGGACGCGGCTGTTGCTCCGCGGCGATACGGACTCGACCCGCTCCTACCCACCCCGGGGCACTATAGCATGTCGGGAATAAGGGGGGACATACTGGACATAGGATTGCATACTTGGTCACCCGATGCTATGGAGGGTCCCACTCCAGTATCCGCTTCGATTCATGCAGCTACTATGGTAACAGCTGGCGTTTTTATGATAGCAAGGTGCTCCCCTTTATTTGAATACCCACCTACAGCTTTGATTGTTATTACTTCTGCAGGAGCTACGACGTCATTCCTTGCGGCAACCACTGGAATATTACAGAACGATCTAAAGAGGGTCATAGCTTATTCAACTTGCAGTCAATTAGGCTATATGATCTTTGCTTGCGGCATCTCTAACTATTCGGTTAGCGTCTTTCACTTAATGAATCACGCGTTTTTCAAAGCATTACTATTCCTGAGTGCGGGTTCGGTGATTCATGCCATGTCGGATGAGCAAGATATGCGGAAGATGGGAGGGCTTGCCTCCTCGTTCCCTTTTACCTATGCCATGATGCTCATGGGCAGCTTATCTCTAATTGGATTCCCTTTTCTAACTGGATTTTATTCCAAAGATGTGATCTTAGAGCTCGCTTACACTAAGTATACCATCAGTGGGAACTTTGCTTTCTGGTTGGGAAGTGTCTCTGTCCTTTTCACTTCTTATTACTCCTTTCGTTTACTTTTTCTAACATTTCTAGTACCAACAAATTCATTCGGGCGAGACATCTTACGATGTCATGATGCGCCCATTCCTATGGCCATTCCTTCAATACTTCTGGCTCTCGGGAGTCTCTTTGTAGGATACTTGGCCAAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko05012 map00190 map01100 map04714 map04723 map05012
Pfam Domains
Protein Families
NADH5_C Proton_antipo_M Proton_antipo_N

Protein Analysis

291

Amino Acids

31.5

Weight (kDa)

7.03

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 43 - 228 6.1e-53 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017099)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00513
fragaria_vesca FvH4_6g34420 FvH4_c1g00470
malus_domestica MD13G1126900.v1.1
pyrus_communis pycom12401g00270 pycom17g12740
rosa_chinensis RchiOBHm_MTg0498221
rosa_laevigata RLG00000000086 RLG00000000118 RLG00000000360 RLG00000000372 RLG00000000388
rosa_wichuraiana Rw0G023320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 302
AccBSI CCGCTC 1 cut(s) 70
AccI GTMKAC 1 cut(s) 656
AccII CGCG 3 cut(s) 35, 49, 446
AccIII TCCGGA 1 cut(s) 28
AciI CCGC 9 cut(s) 35, 47, 49, 68, 180, 311, 399, 476, 517
AclWI GGATC 1 cut(s) 20
AcoI YGGCCR 2 cut(s) 810, 861
AcsI RAATTY 1 cut(s) 760
AcyI GRCGYC 1 cut(s) 299
AfaI GTAC 1 cut(s) 753
AfiI CCNNNNNNNGG 4 cut(s) 83, 84, 475, 666
AflIII ACRYGT 1 cut(s) 444
AgsI TTSAA 4 cut(s) 251, 363, 454, 822
AluBI AGCT 7 cut(s) 197, 212, 266, 293, 356, 579, 640
AluI AGCT 7 cut(s) 197, 212, 266, 293, 356, 579, 640
Alw21I GWGCWC 2 cut(s) 239, 642
Alw26I GTCTC 3 cut(s) 700, 768, 848
AlwI GGATC 1 cut(s) 20
Ama87I CYCGRG 2 cut(s) 82, 836
Aor13HI TCCGGA 1 cut(s) 28
AoxI GGCC 2 cut(s) 810, 861
ApeKI GCWGC 2 cut(s) 194, 576
ApoI RAATTY 1 cut(s) 760
AspLEI GCGC 1 cut(s) 799
AspS9I GGNCC 1 cut(s) 164
AsuC2I CCSGG 2 cut(s) 83, 84
AsuHPI GGTGA 2 cut(s) 138, 496
AvaI CYCGRG 2 cut(s) 82, 836
AvaII GGWCC 1 cut(s) 164
BaeGI GKGCMC 1 cut(s) 90
BaeI ACNNNNGTAYC 2 cut(s) 159, 192
BalI TGGCCA 2 cut(s) 812, 863
BanII GRGCYC 1 cut(s) 642
Bbv12I GWGCWC 2 cut(s) 239, 642
BbvI GCAGC 2 cut(s) 206, 588
BccI CCATC 2 cut(s) 517, 668
BciVI GTATCC 2 cut(s) 187, 848
BcnI CCSGG 2 cut(s) 83, 84
BcoDI GTCTC 3 cut(s) 700, 768, 848
BfaI CTAG 1 cut(s) 749
BfmI CTRYAG 3 cut(s) 91, 261, 285
BfuI GTATCC 2 cut(s) 187, 848
BisI GCNGC 6 cut(s) 36, 50, 195, 312, 400, 577
BlsI GCNGC 6 cut(s) 37, 51, 196, 313, 401, 578
Bme1390I CCNGG 2 cut(s) 83, 84
Bme18I GGWCC 1 cut(s) 164
BmeT110I CYCGRG 2 cut(s) 82, 836
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 2 cut(s) 165, 166
BmrFI CCNGG 2 cut(s) 83, 84
BmsI GCATC 4 cut(s) 142, 411, 555, 784
BpmI CTGGAG 1 cut(s) 156
BpuMI CCSGG 2 cut(s) 83, 84
BsaHI GRCGYC 1 cut(s) 299
BsaJI CCNNGG 4 cut(s) 47, 81, 82, 83
BsaWI WCCGGW 1 cut(s) 28
Bsc4I CCNNNNNNNGG 4 cut(s) 83, 84, 475, 666
Bse1I ACTGG 4 cut(s) 126, 173, 325, 613
BseAI TCCGGA 1 cut(s) 28
BseDI CCNNGG 4 cut(s) 47, 81, 82, 83
BseGI GGATG 1 cut(s) 508
BseLI CCNNNNNNNGG 4 cut(s) 83, 84, 475, 666
BseMII CTCAG 1 cut(s) 461
BseNI ACTGG 4 cut(s) 126, 173, 325, 613
BseRI GAGGAG 1 cut(s) 529
BseSI GKGCMC 1 cut(s) 90
BseXI GCAGC 2 cut(s) 206, 588
Bsh1236I CGCG 3 cut(s) 35, 49, 446
BshFI GGCC 2 cut(s) 812, 863
BsiHKAI GWGCWC 2 cut(s) 239, 642
BsiHKCI CYCGRG 2 cut(s) 82, 836
BsiSI CCGG 2 cut(s) 29, 83
BslFI GGGAC 2 cut(s) 128, 150
BslI CCNNNNNNNGG 4 cut(s) 83, 84, 475, 666
BsmAI GTCTC 3 cut(s) 700, 768, 848
BsmFI GGGAC 2 cut(s) 128, 150
BsnI GGCC 2 cut(s) 812, 863
BsoBI CYCGRG 2 cut(s) 82, 836
Bsp1286I GDGCHC 3 cut(s) 90, 239, 642
Bsp13I TCCGGA 1 cut(s) 28
Bsp143I GATC 5 cut(s) 20, 25, 337, 387, 630
BspACI CCGC 9 cut(s) 35, 47, 49, 68, 180, 311, 399, 476, 517
BspANI GGCC 2 cut(s) 812, 863
BspCNI CTCAG 1 cut(s) 462
BspEI TCCGGA 1 cut(s) 28
BspFNI CGCG 3 cut(s) 35, 49, 446
BspHI TCATGA 1 cut(s) 790
BspLI GGNNCC 2 cut(s) 165, 166
BspMAI CTGCAG 1 cut(s) 289
BspPI GGATC 1 cut(s) 20
BsrBI CCGCTC 1 cut(s) 70
BsrI ACTGG 4 cut(s) 126, 173, 325, 613
BssECI CCNNGG 4 cut(s) 47, 81, 82, 83
BssMI GATC 5 cut(s) 20, 25, 337, 387, 630
BssNAI GTATAC 1 cut(s) 657
BssNI GRCGYC 1 cut(s) 299
Bst1107I GTATAC 1 cut(s) 657
BstACI GRCGYC 1 cut(s) 299
BstAPI GCANNNNNTGC 1 cut(s) 514
BstC8I GCNNGC 4 cut(s) 214, 397, 534, 642
BstDEI CTNAG 3 cut(s) 470, 634, 651
BstDSI CCRYGG 1 cut(s) 47
BstEII GGTNACC 1 cut(s) 144
BstF5I GGATG 1 cut(s) 508
BstFNI CGCG 3 cut(s) 35, 49, 446
BstHHI GCGC 1 cut(s) 799
BstKTI GATC 5 cut(s) 23, 28, 340, 390, 633
BstMAI GTCTC 3 cut(s) 700, 768, 848
BstMBI GATC 5 cut(s) 20, 25, 337, 387, 630
BstMWI GCNNNNNNNGC 2 cut(s) 41, 514
BstNSI RCATGY 1 cut(s) 100
BstPI GGTNACC 1 cut(s) 144
BstSCI CCNGG 2 cut(s) 81, 82
BstSFI CTRYAG 3 cut(s) 91, 261, 285
BstSLI GKGCMC 1 cut(s) 90
BstUI CGCG 3 cut(s) 35, 49, 446
BstV1I GCAGC 2 cut(s) 206, 588
BstX2I RGATCY 1 cut(s) 25
BstXI CCANNNNNNTGG 1 cut(s) 808
BstYI RGATCY 1 cut(s) 25
BstZ17I GTATAC 1 cut(s) 657
BsuI GTATCC 2 cut(s) 187, 848
BsuRI GGCC 2 cut(s) 812, 863
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 1 cut(s) 508
BtsIMutI CAGTG 2 cut(s) 318, 670
Cac8I GCNNGC 4 cut(s) 214, 397, 534, 642
CciI TCATGA 1 cut(s) 790
CfoI GCGC 1 cut(s) 799
Cfr13I GGNCC 1 cut(s) 164
Cfr42I CCGCGG 1 cut(s) 50
Cfr9I CCCGGG 1 cut(s) 82
CseI GACGC 2 cut(s) 41, 412
Csp6I GTAC 1 cut(s) 752
CviAII CATG 7 cut(s) 97, 191, 491, 496, 562, 571, 791
CviQI GTAC 1 cut(s) 752
DdeI CTNAG 3 cut(s) 470, 634, 651
DpnI GATC 5 cut(s) 22, 27, 339, 389, 632
DpnII GATC 5 cut(s) 20, 25, 337, 387, 630
EaeI YGGCCR 2 cut(s) 810, 861
Ecl136II GAGCTC 1 cut(s) 640
Eco24I GRGCYC 1 cut(s) 642
Eco47I GGWCC 1 cut(s) 164
Eco53kI GAGCTC 1 cut(s) 640
Eco88I CYCGRG 2 cut(s) 82, 836
Eco91I GGTNACC 1 cut(s) 144
EcoICRI GAGCTC 1 cut(s) 640
EcoO109I RGGNCCY 1 cut(s) 164
EcoO65I GGTNACC 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 642
FaeI CATG 7 cut(s) 100, 194, 494, 499, 565, 574, 794
FaqI GGGAC 2 cut(s) 128, 150
FatI CATG 7 cut(s) 96, 190, 490, 495, 561, 570, 790
FauI CCCGC 2 cut(s) 75, 469
FblI GTMKAC 1 cut(s) 656
Fnu4HI GCNGC 6 cut(s) 36, 50, 195, 312, 400, 577
FokI GGATG 1 cut(s) 515
FriOI GRGCYC 1 cut(s) 642
Fsp4HI GCNGC 6 cut(s) 36, 50, 195, 312, 400, 577
FspBI CTAG 1 cut(s) 749
GlaI GCGC 1 cut(s) 798
GluI GCNGC 6 cut(s) 36, 50, 195, 312, 400, 577
GsuI CTGGAG 1 cut(s) 156
HaeIII GGCC 2 cut(s) 812, 863
HapII CCGG 2 cut(s) 29, 83
HgaI GACGC 2 cut(s) 41, 412
HhaI GCGC 1 cut(s) 799
Hin1I GRCGYC 1 cut(s) 299
Hin1II CATG 7 cut(s) 100, 194, 494, 499, 565, 574, 794
Hin6I GCGC 1 cut(s) 797
HinP1I GCGC 1 cut(s) 797
HinfI GANTC 6 cut(s) 60, 187, 439, 487, 594, 841
HpaII CCGG 2 cut(s) 29, 83
HphI GGTGA 2 cut(s) 138, 496
Hpy166II GTNNAC 2 cut(s) 657, 731
Hpy188I TCNGA 2 cut(s) 25, 502
Hpy188III TCNNGA 5 cut(s) 29, 102, 469, 791, 838
Hpy8I GTNNAC 2 cut(s) 657, 731
Hpy99I CGWCG 1 cut(s) 301
HpyAV CCTTC 1 cut(s) 828
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 4 cut(s) 136, 194, 287, 369
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 514
HpyF3I CTNAG 3 cut(s) 470, 634, 651
HpySE526I ACGT 1 cut(s) 299
Hsp92I GRCGYC 1 cut(s) 299
Hsp92II CATG 7 cut(s) 100, 194, 494, 499, 565, 574, 794
HspAI GCGC 1 cut(s) 797
KflI GGGWCCC 1 cut(s) 164
Kpn2I TCCGGA 1 cut(s) 28
KspI CCGCGG 1 cut(s) 50
Kzo9I GATC 5 cut(s) 20, 25, 337, 387, 630
LmnI GCTCC 4 cut(s) 49, 75, 242, 290
Lsp1109I GCAGC 2 cut(s) 206, 588
LweI GCATC 4 cut(s) 142, 411, 555, 784
MaeI CTAG 1 cut(s) 749
MaeII ACGT 1 cut(s) 299
MaeIII GTNAC 2 cut(s) 144, 205
MalI GATC 5 cut(s) 22, 27, 339, 389, 632
MbiI CCGCTC 1 cut(s) 70
MboI GATC 5 cut(s) 20, 25, 337, 387, 630
MboII GAAGA 1 cut(s) 532
MflI RGATCY 1 cut(s) 25
MhlI GDGCHC 3 cut(s) 90, 239, 642
MlsI TGGCCA 2 cut(s) 812, 863
MluCI AATT 4 cut(s) 4, 374, 588, 760
MluI ACGCGT 1 cut(s) 444
MluNI TGGCCA 2 cut(s) 812, 863
MlyI GAGTC 2 cut(s) 54, 850
MmeI TCCRAC 1 cut(s) 480
MnlI CCTC 5 cut(s) 154, 339, 521, 547, 550
Mox20I TGGCCA 2 cut(s) 812, 863
MroI TCCGGA 1 cut(s) 28
MscI TGGCCA 2 cut(s) 812, 863
MseI TTAA 1 cut(s) 434
MslI CAYNNNNRTG 1 cut(s) 806
Msp20I TGGCCA 2 cut(s) 812, 863
MspA1I CMGCKG 2 cut(s) 49, 212
MspI CCGG 2 cut(s) 29, 83
MspR9I CCNGG 2 cut(s) 83, 84
MvnI CGCG 3 cut(s) 35, 49, 446
MwoI GCNNNNNNNGC 2 cut(s) 41, 514
NciI CCSGG 2 cut(s) 83, 84
NdeII GATC 5 cut(s) 20, 25, 337, 387, 630
NlaIII CATG 7 cut(s) 100, 194, 494, 499, 565, 574, 794
NlaIV GGNNCC 2 cut(s) 165, 166
NmeAIII GCCGAG 1 cut(s) 35
NmuCI GTSAC 1 cut(s) 144
NspI RCATGY 1 cut(s) 100
PagI TCATGA 1 cut(s) 790
PfeI GAWTC 4 cut(s) 187, 439, 487, 594
PkrI GCNGC 6 cut(s) 37, 51, 196, 313, 401, 578
PleI GAGTC 2 cut(s) 54, 849
PpsI GAGTC 2 cut(s) 54, 849
PpuMI RGGWCCY 1 cut(s) 164
Psp124BI GAGCTC 1 cut(s) 642
Psp5II RGGWCCY 1 cut(s) 164
PspEI GGTNACC 1 cut(s) 144
PspN4I GGNNCC 2 cut(s) 165, 166
PspPI GGNCC 1 cut(s) 164
PspPPI RGGWCCY 1 cut(s) 164
PstI CTGCAG 1 cut(s) 289
PsuI RGATCY 1 cut(s) 25
PvuII CAGCTG 1 cut(s) 212
RsaI GTAC 1 cut(s) 753
RsaNI GTAC 1 cut(s) 752
RseI CAYNNNNRTG 1 cut(s) 806
SacI GAGCTC 1 cut(s) 642
SacII CCGCGG 1 cut(s) 50
SaqAI TTAA 1 cut(s) 434
SatI GCNGC 6 cut(s) 36, 50, 195, 312, 400, 577
Sau3AI GATC 5 cut(s) 20, 25, 337, 387, 630
Sau96I GGNCC 1 cut(s) 164
SchI GAGTC 2 cut(s) 54, 850
ScrFI CCNGG 2 cut(s) 83, 84
SduI GDGCHC 3 cut(s) 90, 239, 642
SfaNI GCATC 4 cut(s) 142, 411, 555, 784
SfcI CTRYAG 3 cut(s) 91, 261, 285
Sfr303I CCGCGG 1 cut(s) 50
SgrBI CCGCGG 1 cut(s) 50
SinI GGWCC 1 cut(s) 164
SmaI CCCGGG 1 cut(s) 84
SmiMI CAYNNNNRTG 1 cut(s) 806
Sse9I AATT 4 cut(s) 4, 374, 588, 760
SsiI CCGC 9 cut(s) 35, 47, 49, 68, 180, 311, 399, 476, 517
SspI AATATT 1 cut(s) 327
SspMI CTAG 1 cut(s) 749
SstI GAGCTC 1 cut(s) 642
StyD4I CCNGG 2 cut(s) 81, 82
TaiI ACGT 1 cut(s) 302
TaqI TCGA 2 cut(s) 63, 185
TasI AATT 4 cut(s) 4, 374, 588, 760
TauI GCSGC 4 cut(s) 38, 52, 314, 402
TfiI GAWTC 4 cut(s) 187, 439, 487, 594
Tru1I TTAA 1 cut(s) 434
Tru9I TTAA 1 cut(s) 434
TscAI CASTG 2 cut(s) 325, 670
TseFI GTSAC 1 cut(s) 144
TseI GCWGC 2 cut(s) 194, 576
Tsp45I GTSAC 1 cut(s) 144
TspDTI ATGAA 5 cut(s) 17, 179, 452, 479, 753
TspGWI ACGGA 1 cut(s) 72
TspMI CCCGGG 1 cut(s) 82
TspRI CASTG 2 cut(s) 325, 670
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 1 cut(s) 760
XceI RCATGY 1 cut(s) 100
XcmI CCANNNNNNNNNTGG 1 cut(s) 568
XmaI CCCGGG 1 cut(s) 82
XmiI GTMKAC 1 cut(s) 656
XspI CTAG 1 cut(s) 749
ZraI GACGTC 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.