RLG00000000360

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017099
Physical Location & Seq
Forward (+)
707496 .. 708572
1077 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000360

Sequence Viewer

Length: 1017 bp
ATGTTGGTGACTGGAGATAACTCTCTTCAATTATTCCTAGGATGGGAGGGAGTAGGTCTTGCTTCATATTTGTTAATTCATTTCTGGTTTACACGACTTCAGGCAGATAAAGCAGCTACAAAAGCTATGCCTGTCAATCGAGTAGGTGATTTTGGATTAGCTCCTGGGATTTTGGGCCGTTTTACTCTCTTTCAAACAGTAGACTTTTCAACCATTTTTGCTCGTGCTAGTGCCCCCAGAAATTCTTGGATTTCTTGCAATATGAGATTTAATGCCATAACTCTTATTTGTATTTTACTTTTTATTGGTGCTGTTGGGAAATCTGCACAGATAGGATTGCATACTTGGTCACCCGATGCTATGGAGGGTCCCACTCCAGTATCCGCTTCGATTCATGCAGCTACTATGGTAACAGCTGGCGTTTTTATGATAGCAAGGTGCTCCCCTTTATTTGAATACCCACCTACAGCTTTGATTGTTATTACTTCTGCAGGAGCTACGACGTCATTCCTTGCGGCAACCACTGGAATATTACAGAACGATCTAAAGAGGGTCATAGCTTATTCAACTTGCAGTCAATTAGGCTATATGATCTTTGCTTGCGGCATCTCTAACTATTCGGTTAGCGTCTTTCACTTAATGAATCACGCGTTTTTCAAAGCATTACTATTCCTGAGTGCGGGTTCGGTGATTCATGCCATGTCGGATGAGCAAGATATGCGGAAGATGGGAGGGCTTGCCTCCTCGTTCCCTTTTACCTATGCCATGATGCTCATGGGCAGCTTATCTCTAATTGGATTCCCTTTTCTAACTGGATTTTATTCCAAAGATGTGATCTTAGAGCTCGCTTACACTAAGTATACCATCAGTGGGAACTTTGCTTTCTGGTTGGGAATACCAACAAATTCATTCGGGCGAGACATCTTACGATGTCATGATGCGCCCATTCCTATGGCCATTCCTTCAATACTTCTGGCTCTCGGGAGTCTCTTTGTAGGATACTTGGCCAAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko05012 map00190 map01100 map04714 map04723 map05012
Pfam Domains
Protein Families
Proton_antipo_M Proton_antipo_N

Protein Analysis

339

Amino Acids

36.58

Weight (kDa)

8.72

Isoelectric Point (pI)

30.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 7 - 292 2.1e-74 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017099)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00513
fragaria_vesca FvH4_6g34420 FvH4_c1g00470
malus_domestica MD13G1126900.v1.1
pyrus_communis pycom12401g00270 pycom17g12740
rosa_chinensis RchiOBHm_MTg0498221
rosa_laevigata RLG00000000086 RLG00000000118 RLG00000000360 RLG00000000372 RLG00000000388
rosa_wichuraiana Rw0G023320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 506
AccI GTMKAC 2 cut(s) 201, 860
AccII CGCG 1 cut(s) 650
AciI CCGC 5 cut(s) 384, 515, 603, 680, 721
AcoI YGGCCR 2 cut(s) 954, 1005
AcsI RAATTY 2 cut(s) 241, 904
AcuI CTGAAG 1 cut(s) 83
AcyI GRCGYC 1 cut(s) 503
AfiI CCNNNNNNNGG 3 cut(s) 43, 679, 870
AflIII ACRYGT 1 cut(s) 648
AgsI TTSAA 7 cut(s) 29, 194, 210, 455, 567, 658, 966
AjnI CCWGG 1 cut(s) 163
Alw21I GWGCWC 2 cut(s) 443, 846
Alw26I GTCTC 2 cut(s) 912, 992
Ama87I CYCGRG 1 cut(s) 980
AoxI GGCC 3 cut(s) 175, 954, 1005
ApeKI GCWGC 3 cut(s) 113, 398, 780
ApoI RAATTY 2 cut(s) 241, 904
AspA2I CCTAGG 1 cut(s) 37
AspLEI GCGC 1 cut(s) 943
AspS9I GGNCC 2 cut(s) 175, 368
AsuHPI GGTGA 4 cut(s) 19, 158, 342, 700
AvaI CYCGRG 1 cut(s) 980
AvaII GGWCC 1 cut(s) 368
AvrII CCTAGG 1 cut(s) 37
BaeGI GKGCMC 1 cut(s) 235
BaeI ACNNNNGTAYC 2 cut(s) 363, 396
BalI TGGCCA 2 cut(s) 956, 1007
BanII GRGCYC 1 cut(s) 846
BauI CACGAG 1 cut(s) 222
Bbv12I GWGCWC 2 cut(s) 443, 846
BbvI GCAGC 3 cut(s) 125, 410, 792
BccI CCATC 3 cut(s) 36, 721, 872
BceAI ACGGC 1 cut(s) 162
BciT130I CCWGG 1 cut(s) 165
BciVI GTATCC 2 cut(s) 391, 992
BcoDI GTCTC 2 cut(s) 912, 992
BfaI CTAG 2 cut(s) 38, 228
BfmI CTRYAG 2 cut(s) 465, 489
BfuI GTATCC 2 cut(s) 391, 992
BisI GCNGC 5 cut(s) 114, 399, 516, 604, 781
BlnI CCTAGG 1 cut(s) 37
BlsI GCNGC 5 cut(s) 115, 400, 517, 605, 782
Bme1390I CCNGG 1 cut(s) 165
Bme18I GGWCC 1 cut(s) 368
BmeT110I CYCGRG 1 cut(s) 980
BmgT120I GGNCC 2 cut(s) 175, 368
BmiI GGNNCC 2 cut(s) 369, 370
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 4 cut(s) 346, 615, 759, 928
BpmI CTGGAG 2 cut(s) 33, 360
BsaHI GRCGYC 1 cut(s) 503
BsaJI CCNNGG 2 cut(s) 37, 164
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 679, 870
Bse1I ACTGG 4 cut(s) 16, 377, 529, 817
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 2 cut(s) 37, 164
BseGI GGATG 2 cut(s) 47, 712
BseLI CCNNNNNNNGG 3 cut(s) 43, 679, 870
BseMII CTCAG 1 cut(s) 665
BseNI ACTGG 4 cut(s) 16, 377, 529, 817
BseRI GAGGAG 1 cut(s) 733
BseSI GKGCMC 1 cut(s) 235
BseXI GCAGC 3 cut(s) 125, 410, 792
BsgI GTGCAG 1 cut(s) 309
Bsh1236I CGCG 1 cut(s) 650
BshFI GGCC 3 cut(s) 177, 956, 1007
BsiHKAI GWGCWC 2 cut(s) 443, 846
BsiHKCI CYCGRG 1 cut(s) 980
BslFI GGGAC 1 cut(s) 354
BslI CCNNNNNNNGG 3 cut(s) 43, 679, 870
BsmAI GTCTC 2 cut(s) 912, 992
BsmFI GGGAC 1 cut(s) 354
BsnI GGCC 3 cut(s) 177, 956, 1007
BsoBI CYCGRG 1 cut(s) 980
Bsp1286I GDGCHC 3 cut(s) 235, 443, 846
Bsp143I GATC 3 cut(s) 541, 591, 834
BspACI CCGC 5 cut(s) 384, 515, 603, 680, 721
BspANI GGCC 3 cut(s) 177, 956, 1007
BspCNI CTCAG 1 cut(s) 666
BspFNI CGCG 1 cut(s) 650
BspHI TCATGA 1 cut(s) 934
BspLI GGNNCC 2 cut(s) 369, 370
BspMAI CTGCAG 1 cut(s) 493
BsrI ACTGG 4 cut(s) 16, 377, 529, 817
BssECI CCNNGG 2 cut(s) 37, 164
BssMI GATC 3 cut(s) 541, 591, 834
BssNAI GTATAC 1 cut(s) 861
BssNI GRCGYC 1 cut(s) 503
BssSI CACGAG 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 37
Bst1107I GTATAC 1 cut(s) 861
Bst2BI CACGAG 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 1 cut(s) 199
Bst6I CTCTTC 1 cut(s) 30
BstACI GRCGYC 1 cut(s) 503
BstAPI GCANNNNNTGC 1 cut(s) 718
BstC8I GCNNGC 4 cut(s) 418, 601, 738, 846
BstDEI CTNAG 3 cut(s) 674, 838, 855
BstEII GGTNACC 1 cut(s) 348
BstF5I GGATG 2 cut(s) 47, 712
BstFNI CGCG 1 cut(s) 650
BstHHI GCGC 1 cut(s) 943
BstKTI GATC 3 cut(s) 544, 594, 837
BstMAI GTCTC 2 cut(s) 912, 992
BstMBI GATC 3 cut(s) 541, 591, 834
BstMWI GCNNNNNNNGC 3 cut(s) 110, 122, 718
BstNI CCWGG 1 cut(s) 165
BstPI GGTNACC 1 cut(s) 348
BstSCI CCNGG 1 cut(s) 163
BstSFI CTRYAG 2 cut(s) 465, 489
BstSLI GKGCMC 1 cut(s) 235
BstUI CGCG 1 cut(s) 650
BstV1I GCAGC 3 cut(s) 125, 410, 792
BstXI CCANNNNNNTGG 1 cut(s) 952
BstZ17I GTATAC 1 cut(s) 861
BsuI GTATCC 2 cut(s) 391, 992
BsuRI GGCC 3 cut(s) 177, 956, 1007
BtsCI GGATG 2 cut(s) 47, 712
BtsIMutI CAGTG 2 cut(s) 522, 874
Cac8I GCNNGC 4 cut(s) 418, 601, 738, 846
CciI TCATGA 1 cut(s) 934
CfoI GCGC 1 cut(s) 943
Cfr13I GGNCC 2 cut(s) 175, 368
CseI GACGC 1 cut(s) 616
CviAII CATG 6 cut(s) 395, 695, 700, 766, 775, 935
DdeI CTNAG 3 cut(s) 674, 838, 855
DpnI GATC 3 cut(s) 543, 593, 836
DpnII GATC 3 cut(s) 541, 591, 834
EaeI YGGCCR 2 cut(s) 954, 1005
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 844
Eco130I CCWWGG 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 846
Eco47I GGWCC 1 cut(s) 368
Eco53kI GAGCTC 1 cut(s) 844
Eco57I CTGAAG 1 cut(s) 83
Eco88I CYCGRG 1 cut(s) 980
Eco91I GGTNACC 1 cut(s) 348
EcoICRI GAGCTC 1 cut(s) 844
EcoO109I RGGNCCY 1 cut(s) 368
EcoO65I GGTNACC 1 cut(s) 348
EcoRII CCWGG 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 37
EcoT38I GRGCYC 1 cut(s) 846
ErhI CCWWGG 1 cut(s) 37
FaeI CATG 6 cut(s) 398, 698, 703, 769, 778, 938
FaqI GGGAC 1 cut(s) 354
FatI CATG 6 cut(s) 394, 694, 699, 765, 774, 934
FauI CCCGC 1 cut(s) 673
FblI GTMKAC 2 cut(s) 201, 860
Fnu4HI GCNGC 5 cut(s) 114, 399, 516, 604, 781
FokI GGATG 2 cut(s) 54, 719
FriOI GRGCYC 1 cut(s) 846
Fsp4HI GCNGC 5 cut(s) 114, 399, 516, 604, 781
FspBI CTAG 2 cut(s) 38, 228
GlaI GCGC 1 cut(s) 942
GluI GCNGC 5 cut(s) 114, 399, 516, 604, 781
GsuI CTGGAG 2 cut(s) 33, 360
HaeIII GGCC 3 cut(s) 177, 956, 1007
HgaI GACGC 1 cut(s) 616
HhaI GCGC 1 cut(s) 943
Hin1I GRCGYC 1 cut(s) 503
Hin1II CATG 6 cut(s) 398, 698, 703, 769, 778, 938
Hin6I GCGC 1 cut(s) 941
HinP1I GCGC 1 cut(s) 941
HinfI GANTC 5 cut(s) 391, 643, 691, 798, 985
HphI GGTGA 4 cut(s) 19, 158, 342, 700
Hpy166II GTNNAC 3 cut(s) 90, 202, 861
Hpy188I TCNGA 1 cut(s) 706
Hpy188III TCNNGA 3 cut(s) 673, 935, 982
Hpy8I GTNNAC 3 cut(s) 90, 202, 861
Hpy99I CGWCG 1 cut(s) 505
HpyAV CCTTC 1 cut(s) 972
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4IV ACGT 1 cut(s) 503
HpyCH4V TGCA 6 cut(s) 258, 326, 340, 398, 491, 573
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 122, 718
HpyF3I CTNAG 3 cut(s) 674, 838, 855
HpySE526I ACGT 1 cut(s) 503
Hsp92I GRCGYC 1 cut(s) 503
Hsp92II CATG 6 cut(s) 398, 698, 703, 769, 778, 938
HspAI GCGC 1 cut(s) 941
KflI GGGWCCC 1 cut(s) 368
Kzo9I GATC 3 cut(s) 541, 591, 834
LmnI GCTCC 3 cut(s) 166, 446, 494
Lsp1109I GCAGC 3 cut(s) 125, 410, 792
LweI GCATC 4 cut(s) 346, 615, 759, 928
MaeI CTAG 2 cut(s) 38, 228
MaeII ACGT 1 cut(s) 503
MaeIII GTNAC 3 cut(s) 7, 348, 409
MalI GATC 3 cut(s) 543, 593, 836
MboI GATC 3 cut(s) 541, 591, 834
MboII GAAGA 2 cut(s) 17, 736
MhlI GDGCHC 3 cut(s) 235, 443, 846
MlsI TGGCCA 2 cut(s) 956, 1007
MluCI AATT 6 cut(s) 29, 75, 241, 578, 792, 904
MluI ACGCGT 1 cut(s) 648
MluNI TGGCCA 2 cut(s) 956, 1007
MlyI GAGTC 1 cut(s) 994
MmeI TCCRAC 1 cut(s) 684
MnlI CCTC 6 cut(s) 40, 358, 543, 725, 751, 754
Mox20I TGGCCA 2 cut(s) 956, 1007
MscI TGGCCA 2 cut(s) 956, 1007
MseI TTAA 3 cut(s) 74, 270, 638
MslI CAYNNNNRTG 1 cut(s) 950
Msp20I TGGCCA 2 cut(s) 956, 1007
MspA1I CMGCKG 1 cut(s) 416
MspR9I CCNGG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 165
MvnI CGCG 1 cut(s) 650
MwoI GCNNNNNNNGC 3 cut(s) 110, 122, 718
NdeII GATC 3 cut(s) 541, 591, 834
NlaIII CATG 6 cut(s) 398, 698, 703, 769, 778, 938
NlaIV GGNNCC 2 cut(s) 369, 370
NmuCI GTSAC 2 cut(s) 7, 348
PagI TCATGA 1 cut(s) 934
PfeI GAWTC 4 cut(s) 391, 643, 691, 798
PkrI GCNGC 5 cut(s) 115, 400, 517, 605, 782
PleI GAGTC 1 cut(s) 993
PpsI GAGTC 1 cut(s) 993
PpuMI RGGWCCY 1 cut(s) 368
Psp124BI GAGCTC 1 cut(s) 846
Psp5II RGGWCCY 1 cut(s) 368
Psp6I CCWGG 1 cut(s) 163
PspEI GGTNACC 1 cut(s) 348
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 2 cut(s) 369, 370
PspPI GGNCC 2 cut(s) 175, 368
PspPPI RGGWCCY 1 cut(s) 368
PstI CTGCAG 1 cut(s) 493
PvuII CAGCTG 1 cut(s) 416
RseI CAYNNNNRTG 1 cut(s) 950
SacI GAGCTC 1 cut(s) 846
SaqAI TTAA 3 cut(s) 74, 270, 638
SatI GCNGC 5 cut(s) 114, 399, 516, 604, 781
Sau3AI GATC 3 cut(s) 541, 591, 834
Sau96I GGNCC 2 cut(s) 175, 368
SchI GAGTC 1 cut(s) 994
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 3 cut(s) 235, 443, 846
SfaNI GCATC 4 cut(s) 346, 615, 759, 928
SfcI CTRYAG 2 cut(s) 465, 489
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 1 cut(s) 950
Sse9I AATT 6 cut(s) 29, 75, 241, 578, 792, 904
SsiI CCGC 5 cut(s) 384, 515, 603, 680, 721
SspI AATATT 1 cut(s) 531
SspMI CTAG 2 cut(s) 38, 228
SstI GAGCTC 1 cut(s) 846
StyD4I CCNGG 1 cut(s) 163
StyI CCWWGG 1 cut(s) 37
TaaI ACNGT 1 cut(s) 199
TaiI ACGT 1 cut(s) 506
TaqI TCGA 2 cut(s) 139, 389
TasI AATT 6 cut(s) 29, 75, 241, 578, 792, 904
TauI GCSGC 2 cut(s) 518, 606
TfiI GAWTC 4 cut(s) 391, 643, 691, 798
Tru1I TTAA 3 cut(s) 74, 270, 638
Tru9I TTAA 3 cut(s) 74, 270, 638
TscAI CASTG 2 cut(s) 529, 874
TseFI GTSAC 2 cut(s) 7, 348
TseI GCWGC 3 cut(s) 113, 398, 780
Tsp45I GTSAC 2 cut(s) 7, 348
TspDTI ATGAA 6 cut(s) 54, 68, 383, 656, 683, 897
TspRI CASTG 2 cut(s) 529, 874
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 2 cut(s) 241, 904
XcmI CCANNNNNNNNNTGG 1 cut(s) 772
XmaJI CCTAGG 1 cut(s) 37
XmiI GTMKAC 2 cut(s) 201, 860
XspI CTAG 2 cut(s) 38, 228
ZraI GACGTC 1 cut(s) 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.