RLG00000000372

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017099
Physical Location & Seq
Reverse (-)
757617 .. 759640
2024 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000372

Sequence Viewer

Length: 1101 bp
ATGAATTGCCGAGTAGATAGATCAGATCCGGACGCGGCTGTTGCTCCGCGGCGATACGGACTCGACCCGCTCCTACCCACCCCGGGGCACTATAGCATGTCGGGAATAAGGGGGGACATACTGGACGCAGATAAAGCAGCTACAAAAGCTATGCCTGTCAATCGAGTAGGTGATTTTGGATTAGCTCCTGGGATTTTGGGCCGTTTTACTCTCTTTCAAACAGTAGACTTTTCAACCATTTTTGCTCGTGCTAGTGCCCCCAGAAATTCTTGGATTTCTTGCAATATGAGATTTAATGCCATAACTCTTATTTGTATTTTACTTTTTATTGGTGCTGTTGGGAAATCTGCACAGATAGGATTGCATACTTGGTCACCCGATGCTATGGAGGGTCCCACTCCAGTATCCGCTTCGATTCATGCAGCTACTATGGTAACAGCTGGCGTTTTTATGATAGCAAGGTGCTCCCCTTTATTTGAATACCCACCTACAGCTTTGATTGTTATTACTTCTGCAGGAGCTACGACGTCATTCCTTGCGGCAACCACTGGAATATTACAGAACGATCTAAAGAGGGTCATAGCTTATTCAACTTGCAGTCAATTAGGCTATATGATCTTTGCTTGCGGCATCTCTAACTATTCGGTTAGCGTCTTTCACTTAATGAATCACGCGTTTTTCAAAGCATTACTATTCCTGAGTGCGGGTTCGGTGATTCATGCCATGTCGGATGAGCAAGATATGCGGAAGATGGGAGGGCTTGCCTCCTCGTTCCCTTTTACCTATGCCATGATGCTCATGGGCAGCTTATCTCTAATTGGATTCCCTTTTCTAACTGGATTTTATTCCAAAGATGTGATCTTAGAGCTCGCTTACACTAAGTATACCATCAGTGGGAACTTTGCTTTCTGGTTGGGAAGTGTCTCTGTCCTTTTCACTTCTTATTACTCCTTTCGTTTACTTTTTCTAACATTTCTAGTACCAACAAATTCATTCGGGCGAGACATCTTACGATGTCATGATGCGCCCATTCCTATGGCCATTCCTTCAATACTTCTGGCTCTCGGGAGTCTCTTTGTAGGATACTTGGCCAAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko05012 map00190 map01100 map04714 map04723 map05012
Pfam Domains
Protein Families
Proton_antipo_M Proton_antipo_N

Protein Analysis

367

Amino Acids

39.63

Weight (kDa)

8.8

Isoelectric Point (pI)

34.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 31 - 304 2.2e-65 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017099)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00513
fragaria_vesca FvH4_6g34420 FvH4_c1g00470
malus_domestica MD13G1126900.v1.1
pyrus_communis pycom12401g00270 pycom17g12740
rosa_chinensis RchiOBHm_MTg0498221
rosa_laevigata RLG00000000086 RLG00000000118 RLG00000000360 RLG00000000372 RLG00000000388
rosa_wichuraiana Rw0G023320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 530
AccBSI CCGCTC 1 cut(s) 70
AccI GTMKAC 2 cut(s) 225, 884
AccII CGCG 3 cut(s) 35, 49, 674
AccIII TCCGGA 1 cut(s) 28
AciI CCGC 9 cut(s) 35, 47, 49, 68, 408, 539, 627, 704, 745
AclWI GGATC 1 cut(s) 20
AcoI YGGCCR 2 cut(s) 1038, 1089
AcsI RAATTY 2 cut(s) 265, 988
AcyI GRCGYC 1 cut(s) 527
AfaI GTAC 1 cut(s) 981
AfiI CCNNNNNNNGG 4 cut(s) 83, 84, 703, 894
AflIII ACRYGT 1 cut(s) 672
AgsI TTSAA 6 cut(s) 218, 234, 479, 591, 682, 1050
AjnI CCWGG 1 cut(s) 187
Alw21I GWGCWC 2 cut(s) 467, 870
Alw26I GTCTC 3 cut(s) 928, 996, 1076
AlwI GGATC 1 cut(s) 20
Ama87I CYCGRG 2 cut(s) 82, 1064
Aor13HI TCCGGA 1 cut(s) 28
AoxI GGCC 3 cut(s) 199, 1038, 1089
ApeKI GCWGC 3 cut(s) 137, 422, 804
ApoI RAATTY 2 cut(s) 265, 988
AspLEI GCGC 1 cut(s) 1027
AspS9I GGNCC 2 cut(s) 199, 392
AsuC2I CCSGG 2 cut(s) 83, 84
AsuHPI GGTGA 3 cut(s) 182, 366, 724
AvaI CYCGRG 2 cut(s) 82, 1064
AvaII GGWCC 1 cut(s) 392
BaeGI GKGCMC 2 cut(s) 90, 259
BaeI ACNNNNGTAYC 2 cut(s) 387, 420
BalI TGGCCA 2 cut(s) 1040, 1091
BanII GRGCYC 1 cut(s) 870
BauI CACGAG 1 cut(s) 246
Bbv12I GWGCWC 2 cut(s) 467, 870
BbvI GCAGC 3 cut(s) 149, 434, 816
BccI CCATC 2 cut(s) 745, 896
BceAI ACGGC 1 cut(s) 186
BciT130I CCWGG 1 cut(s) 189
BciVI GTATCC 2 cut(s) 415, 1076
BcnI CCSGG 2 cut(s) 83, 84
BcoDI GTCTC 3 cut(s) 928, 996, 1076
BfaI CTAG 2 cut(s) 252, 977
BfmI CTRYAG 3 cut(s) 91, 489, 513
BfuI GTATCC 2 cut(s) 415, 1076
BisI GCNGC 7 cut(s) 36, 50, 138, 423, 540, 628, 805
BlsI GCNGC 7 cut(s) 37, 51, 139, 424, 541, 629, 806
Bme1390I CCNGG 3 cut(s) 83, 84, 189
Bme18I GGWCC 1 cut(s) 392
BmeT110I CYCGRG 2 cut(s) 82, 1064
BmgT120I GGNCC 2 cut(s) 199, 392
BmiI GGNNCC 2 cut(s) 393, 394
BmrFI CCNGG 3 cut(s) 83, 84, 189
BmsI GCATC 4 cut(s) 370, 639, 783, 1012
BpmI CTGGAG 1 cut(s) 384
BpuMI CCSGG 2 cut(s) 83, 84
BsaHI GRCGYC 1 cut(s) 527
BsaJI CCNNGG 5 cut(s) 47, 81, 82, 83, 188
BsaWI WCCGGW 1 cut(s) 28
Bsc4I CCNNNNNNNGG 4 cut(s) 83, 84, 703, 894
Bse1I ACTGG 4 cut(s) 126, 401, 553, 841
BseAI TCCGGA 1 cut(s) 28
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 5 cut(s) 47, 81, 82, 83, 188
BseGI GGATG 1 cut(s) 736
BseLI CCNNNNNNNGG 4 cut(s) 83, 84, 703, 894
BseMII CTCAG 1 cut(s) 689
BseNI ACTGG 4 cut(s) 126, 401, 553, 841
BseRI GAGGAG 1 cut(s) 757
BseSI GKGCMC 2 cut(s) 90, 259
BseXI GCAGC 3 cut(s) 149, 434, 816
BsgI GTGCAG 1 cut(s) 333
Bsh1236I CGCG 3 cut(s) 35, 49, 674
BshFI GGCC 3 cut(s) 201, 1040, 1091
BsiHKAI GWGCWC 2 cut(s) 467, 870
BsiHKCI CYCGRG 2 cut(s) 82, 1064
BsiSI CCGG 2 cut(s) 29, 83
BslFI GGGAC 2 cut(s) 128, 378
BslI CCNNNNNNNGG 4 cut(s) 83, 84, 703, 894
BsmAI GTCTC 3 cut(s) 928, 996, 1076
BsmFI GGGAC 2 cut(s) 128, 378
BsnI GGCC 3 cut(s) 201, 1040, 1091
BsoBI CYCGRG 2 cut(s) 82, 1064
Bsp1286I GDGCHC 4 cut(s) 90, 259, 467, 870
Bsp13I TCCGGA 1 cut(s) 28
Bsp143I GATC 5 cut(s) 20, 25, 565, 615, 858
BspACI CCGC 9 cut(s) 35, 47, 49, 68, 408, 539, 627, 704, 745
BspANI GGCC 3 cut(s) 201, 1040, 1091
BspCNI CTCAG 1 cut(s) 690
BspEI TCCGGA 1 cut(s) 28
BspFNI CGCG 3 cut(s) 35, 49, 674
BspHI TCATGA 1 cut(s) 1018
BspLI GGNNCC 2 cut(s) 393, 394
BspMAI CTGCAG 1 cut(s) 517
BspPI GGATC 1 cut(s) 20
BsrBI CCGCTC 1 cut(s) 70
BsrI ACTGG 4 cut(s) 126, 401, 553, 841
BssECI CCNNGG 5 cut(s) 47, 81, 82, 83, 188
BssMI GATC 5 cut(s) 20, 25, 565, 615, 858
BssNAI GTATAC 1 cut(s) 885
BssNI GRCGYC 1 cut(s) 527
BssSI CACGAG 1 cut(s) 246
Bst1107I GTATAC 1 cut(s) 885
Bst2BI CACGAG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 1 cut(s) 223
BstACI GRCGYC 1 cut(s) 527
BstAPI GCANNNNNTGC 1 cut(s) 742
BstC8I GCNNGC 4 cut(s) 442, 625, 762, 870
BstDEI CTNAG 3 cut(s) 698, 862, 879
BstDSI CCRYGG 1 cut(s) 47
BstEII GGTNACC 1 cut(s) 372
BstF5I GGATG 1 cut(s) 736
BstFNI CGCG 3 cut(s) 35, 49, 674
BstHHI GCGC 1 cut(s) 1027
BstKTI GATC 5 cut(s) 23, 28, 568, 618, 861
BstMAI GTCTC 3 cut(s) 928, 996, 1076
BstMBI GATC 5 cut(s) 20, 25, 565, 615, 858
BstMWI GCNNNNNNNGC 4 cut(s) 41, 134, 146, 742
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 1 cut(s) 100
BstPI GGTNACC 1 cut(s) 372
BstSCI CCNGG 3 cut(s) 81, 82, 187
BstSFI CTRYAG 3 cut(s) 91, 489, 513
BstSLI GKGCMC 2 cut(s) 90, 259
BstUI CGCG 3 cut(s) 35, 49, 674
BstV1I GCAGC 3 cut(s) 149, 434, 816
BstX2I RGATCY 1 cut(s) 25
BstXI CCANNNNNNTGG 1 cut(s) 1036
BstYI RGATCY 1 cut(s) 25
BstZ17I GTATAC 1 cut(s) 885
BsuI GTATCC 2 cut(s) 415, 1076
BsuRI GGCC 3 cut(s) 201, 1040, 1091
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 1 cut(s) 736
BtsIMutI CAGTG 2 cut(s) 546, 898
Cac8I GCNNGC 4 cut(s) 442, 625, 762, 870
CciI TCATGA 1 cut(s) 1018
CfoI GCGC 1 cut(s) 1027
Cfr13I GGNCC 2 cut(s) 199, 392
Cfr42I CCGCGG 1 cut(s) 50
Cfr9I CCCGGG 1 cut(s) 82
CseI GACGC 3 cut(s) 41, 134, 640
Csp6I GTAC 1 cut(s) 980
CviAII CATG 7 cut(s) 97, 419, 719, 724, 790, 799, 1019
CviQI GTAC 1 cut(s) 980
DdeI CTNAG 3 cut(s) 698, 862, 879
DpnI GATC 5 cut(s) 22, 27, 567, 617, 860
DpnII GATC 5 cut(s) 20, 25, 565, 615, 858
EaeI YGGCCR 2 cut(s) 1038, 1089
Ecl136II GAGCTC 1 cut(s) 868
Eco24I GRGCYC 1 cut(s) 870
Eco47I GGWCC 1 cut(s) 392
Eco53kI GAGCTC 1 cut(s) 868
Eco88I CYCGRG 2 cut(s) 82, 1064
Eco91I GGTNACC 1 cut(s) 372
EcoICRI GAGCTC 1 cut(s) 868
EcoO109I RGGNCCY 1 cut(s) 392
EcoO65I GGTNACC 1 cut(s) 372
EcoRII CCWGG 1 cut(s) 187
EcoT38I GRGCYC 1 cut(s) 870
FaeI CATG 7 cut(s) 100, 422, 722, 727, 793, 802, 1022
FaqI GGGAC 2 cut(s) 128, 378
FatI CATG 7 cut(s) 96, 418, 718, 723, 789, 798, 1018
FauI CCCGC 2 cut(s) 75, 697
FblI GTMKAC 2 cut(s) 225, 884
Fnu4HI GCNGC 7 cut(s) 36, 50, 138, 423, 540, 628, 805
FokI GGATG 1 cut(s) 743
FriOI GRGCYC 1 cut(s) 870
Fsp4HI GCNGC 7 cut(s) 36, 50, 138, 423, 540, 628, 805
FspBI CTAG 2 cut(s) 252, 977
GlaI GCGC 1 cut(s) 1026
GluI GCNGC 7 cut(s) 36, 50, 138, 423, 540, 628, 805
GsuI CTGGAG 1 cut(s) 384
HaeIII GGCC 3 cut(s) 201, 1040, 1091
HapII CCGG 2 cut(s) 29, 83
HgaI GACGC 3 cut(s) 41, 134, 640
HhaI GCGC 1 cut(s) 1027
Hin1I GRCGYC 1 cut(s) 527
Hin1II CATG 7 cut(s) 100, 422, 722, 727, 793, 802, 1022
Hin6I GCGC 1 cut(s) 1025
HinP1I GCGC 1 cut(s) 1025
HinfI GANTC 6 cut(s) 60, 415, 667, 715, 822, 1069
HpaII CCGG 2 cut(s) 29, 83
HphI GGTGA 3 cut(s) 182, 366, 724
Hpy166II GTNNAC 3 cut(s) 226, 885, 959
Hpy188I TCNGA 2 cut(s) 25, 730
Hpy188III TCNNGA 5 cut(s) 29, 102, 697, 1019, 1066
Hpy8I GTNNAC 3 cut(s) 226, 885, 959
Hpy99I CGWCG 1 cut(s) 529
HpyAV CCTTC 1 cut(s) 1056
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 1 cut(s) 527
HpyCH4V TGCA 6 cut(s) 282, 350, 364, 422, 515, 597
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 134, 146, 742
HpyF3I CTNAG 3 cut(s) 698, 862, 879
HpySE526I ACGT 1 cut(s) 527
Hsp92I GRCGYC 1 cut(s) 527
Hsp92II CATG 7 cut(s) 100, 422, 722, 727, 793, 802, 1022
HspAI GCGC 1 cut(s) 1025
KflI GGGWCCC 1 cut(s) 392
Kpn2I TCCGGA 1 cut(s) 28
KspI CCGCGG 1 cut(s) 50
Kzo9I GATC 5 cut(s) 20, 25, 565, 615, 858
LmnI GCTCC 5 cut(s) 49, 75, 190, 470, 518
Lsp1109I GCAGC 3 cut(s) 149, 434, 816
LweI GCATC 4 cut(s) 370, 639, 783, 1012
MaeI CTAG 2 cut(s) 252, 977
MaeII ACGT 1 cut(s) 527
MaeIII GTNAC 2 cut(s) 372, 433
MalI GATC 5 cut(s) 22, 27, 567, 617, 860
MbiI CCGCTC 1 cut(s) 70
MboI GATC 5 cut(s) 20, 25, 565, 615, 858
MboII GAAGA 1 cut(s) 760
MflI RGATCY 1 cut(s) 25
MhlI GDGCHC 4 cut(s) 90, 259, 467, 870
MlsI TGGCCA 2 cut(s) 1040, 1091
MluCI AATT 5 cut(s) 4, 265, 602, 816, 988
MluI ACGCGT 1 cut(s) 672
MluNI TGGCCA 2 cut(s) 1040, 1091
MlyI GAGTC 2 cut(s) 54, 1078
MmeI TCCRAC 1 cut(s) 708
MnlI CCTC 5 cut(s) 382, 567, 749, 775, 778
Mox20I TGGCCA 2 cut(s) 1040, 1091
MroI TCCGGA 1 cut(s) 28
MscI TGGCCA 2 cut(s) 1040, 1091
MseI TTAA 2 cut(s) 294, 662
MslI CAYNNNNRTG 1 cut(s) 1034
Msp20I TGGCCA 2 cut(s) 1040, 1091
MspA1I CMGCKG 2 cut(s) 49, 440
MspI CCGG 2 cut(s) 29, 83
MspR9I CCNGG 3 cut(s) 83, 84, 189
MvaI CCWGG 1 cut(s) 189
MvnI CGCG 3 cut(s) 35, 49, 674
MwoI GCNNNNNNNGC 4 cut(s) 41, 134, 146, 742
NciI CCSGG 2 cut(s) 83, 84
NdeII GATC 5 cut(s) 20, 25, 565, 615, 858
NlaIII CATG 7 cut(s) 100, 422, 722, 727, 793, 802, 1022
NlaIV GGNNCC 2 cut(s) 393, 394
NmeAIII GCCGAG 1 cut(s) 35
NmuCI GTSAC 1 cut(s) 372
NspI RCATGY 1 cut(s) 100
PagI TCATGA 1 cut(s) 1018
PfeI GAWTC 4 cut(s) 415, 667, 715, 822
PkrI GCNGC 7 cut(s) 37, 51, 139, 424, 541, 629, 806
PleI GAGTC 2 cut(s) 54, 1077
PpsI GAGTC 2 cut(s) 54, 1077
PpuMI RGGWCCY 1 cut(s) 392
Psp124BI GAGCTC 1 cut(s) 870
Psp5II RGGWCCY 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 187
PspEI GGTNACC 1 cut(s) 372
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 2 cut(s) 393, 394
PspPI GGNCC 2 cut(s) 199, 392
PspPPI RGGWCCY 1 cut(s) 392
PstI CTGCAG 1 cut(s) 517
PsuI RGATCY 1 cut(s) 25
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 1 cut(s) 981
RsaNI GTAC 1 cut(s) 980
RseI CAYNNNNRTG 1 cut(s) 1034
SacI GAGCTC 1 cut(s) 870
SacII CCGCGG 1 cut(s) 50
SaqAI TTAA 2 cut(s) 294, 662
SatI GCNGC 7 cut(s) 36, 50, 138, 423, 540, 628, 805
Sau3AI GATC 5 cut(s) 20, 25, 565, 615, 858
Sau96I GGNCC 2 cut(s) 199, 392
SchI GAGTC 2 cut(s) 54, 1078
ScrFI CCNGG 3 cut(s) 83, 84, 189
SduI GDGCHC 4 cut(s) 90, 259, 467, 870
SfaNI GCATC 4 cut(s) 370, 639, 783, 1012
SfcI CTRYAG 3 cut(s) 91, 489, 513
Sfr303I CCGCGG 1 cut(s) 50
SgrBI CCGCGG 1 cut(s) 50
SinI GGWCC 1 cut(s) 392
SmaI CCCGGG 1 cut(s) 84
SmiMI CAYNNNNRTG 1 cut(s) 1034
Sse9I AATT 5 cut(s) 4, 265, 602, 816, 988
SsiI CCGC 9 cut(s) 35, 47, 49, 68, 408, 539, 627, 704, 745
SspI AATATT 1 cut(s) 555
SspMI CTAG 2 cut(s) 252, 977
SstI GAGCTC 1 cut(s) 870
StyD4I CCNGG 3 cut(s) 81, 82, 187
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 1 cut(s) 530
TaqI TCGA 3 cut(s) 63, 163, 413
TasI AATT 5 cut(s) 4, 265, 602, 816, 988
TauI GCSGC 4 cut(s) 38, 52, 542, 630
TfiI GAWTC 4 cut(s) 415, 667, 715, 822
Tru1I TTAA 2 cut(s) 294, 662
Tru9I TTAA 2 cut(s) 294, 662
TscAI CASTG 2 cut(s) 553, 898
TseFI GTSAC 1 cut(s) 372
TseI GCWGC 3 cut(s) 137, 422, 804
Tsp45I GTSAC 1 cut(s) 372
TspDTI ATGAA 5 cut(s) 17, 407, 680, 707, 981
TspGWI ACGGA 1 cut(s) 72
TspMI CCCGGG 1 cut(s) 82
TspRI CASTG 2 cut(s) 553, 898
VpaK11BI GGWCC 1 cut(s) 392
XapI RAATTY 2 cut(s) 265, 988
XceI RCATGY 1 cut(s) 100
XcmI CCANNNNNNNNNTGG 1 cut(s) 796
XmaI CCCGGG 1 cut(s) 82
XmiI GTMKAC 2 cut(s) 225, 884
XspI CTAG 2 cut(s) 252, 977
ZraI GACGTC 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.