Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0009114

Physical Location & Seq

Genomic Coordinates Forward (+)

11453212 .. 11457357

Gene Structure 4146 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000001179

Sequence Viewer

CDS Protein Promoter (2k)

Length: 894 bp

ATGTACGGACACGCCCACCCCATGGACATGCAAAACCAGATCCCCGCCGGCGGCGGCAACGACGACGATGCGCCGGGCCCGGAGTCCATCGACAACGCCCACATTCACTACGAAGGCCACACGCTCGAGGACGGCGGGGTCGTCGTCGAGGAGGCCACCTCCGATGACGTTTACATTCACGGCGGAAATACCGACCTGGACATGGCTATTCCTTACGAGGGCTCCAGTCAGCTCACGCTCTCGTTTCGTGGCCAAGTCTACGTCTTTGACTCCGTTACGCCTGATAAGGTTCAAGCTGTGCTATTACTGTTGGGGGGCTCGGAATCATCTTCTGGTGCCCAAGGTGTAGACATGACAACTCAGAATCAGAGGGCCATAACAGAGTTTCCAACAGGCTGTAATCAGCCTCATAGAGCAGCATCGTTAAATAGGTTTCGTCAAAAGAGGAAAGAACGATGCTTTGATAAGAAAGTCAGATATAGTGTCCGTCAAGAAGTTGCCCTCAGGATGCAGCGTAACAAGGGGCAATTTACTTCTTCCAAAAAGTCAGACGGAGATTACAGCTGGGGTTCTGGCCAAGAGTCAAGGCAAGATGACAGCAATGCAGAAACCTCATGCACACATTGTGGAACAAGTTCAAAGAGCACACCAATGATGCGGCGTGGTCCATCTGGTCCAAGGTCTCTTTGCAATGCTTGTGGGCTATTCTGGGCAAATAGGGGGGTTTTGAGGGATATTTCCAAGAGACCACTGGGTCTTTCTCAGGCTGCTGGAGAACAGGGTGAGGCTGAAGCTGATGACTCAGACACTGGAACGGCCATCGTTGTACACAATGCTGCAGATGCTGTTCCCTTATCTAATGGTGATAACAAGGCTTTAGTTGCTGAAACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000976 GO:0000977 GO:0000981 GO:0001012 GO:0001067 GO:0001085 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005667 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CCT GATA tify

Protein Analysis

298

Amino Acids

31.96

Weight (kDa)

5.27

Isoelectric Point (pI)

37.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tify	PF06200	74 - 105	2e-13	tify domain
CCT	PF06203	138 - 180	7.8e-16	CCT motif
Jas_motif	PF09425	138 - 160	2.4e-06	Jas motif
GATA	PF00320	206 - 242	4e-14	GATA zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009114)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24470 AT4G24470 AT4G24470
fragaria_vesca	FvH4_5g34500 FvH4_5g34500
malus_domestica	MD08G1200800.v1.1 MD15G1387700.v1.1
prunus_persica	Prupe.1G534300_v2.0.a1
pyrus_communis	pycom08g17440 pycom15g34800
rosa_chinensis	RchiOBHm_Chr7g0235141
rosa_laevigata	RLG00000001179
rosa_multiflora	Rmu_sc0002239.1_g000022 Rmu_sc0007762.1_g000002
rosa_roxburghii	Rroxscaffold_3G00226250
rosa_rugosa	Rorug07G0288200.1 Rorug07G0288300
rosa_samantha	Rh7AG442900 Rh7BG415600 Rh7CG463800 Rh7DG432600
rosa_wichuraiana	Rw7G036820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	137, 753
AccB1I	GGYRCC	1 cut(s)	335
AccB7I	CCANNNNNTGG	1 cut(s)	22
AccI	GTMKAC	2 cut(s)	258, 348
AciI	CCGC	6 cut(s)	45, 51, 54, 135, 183, 658
AclWI	GGATC	1 cut(s)	34
AcoI	YGGCCR	3 cut(s)	250, 574, 816
AcuI	CTGAAG	1 cut(s)	810
AdeI	CACNNNGTG	1 cut(s)	626
AfaI	GTAC	2 cut(s)	5, 828
AfiI	CCNNNNNNNGG	4 cut(s)	22, 50, 202, 218
AgsI	TTSAA	2 cut(s)	293, 639
AjnI	CCWGG	1 cut(s)	195
AluBI	AGCT	4 cut(s)	232, 296, 564, 794
AluI	AGCT	4 cut(s)	232, 296, 564, 794
Alw21I	GWGCWC	1 cut(s)	647
Alw26I	GTCTC	2 cut(s)	687, 739
AlwI	GGATC	1 cut(s)	34
AlwNI	CAGNNNCTG	2 cut(s)	809, 845
Ama87I	CYCGRG	1 cut(s)	125
AoxI	GGCC	7 cut(s)	76, 115, 153, 250, 372, 574, 816
ApaI	GGGCCC	1 cut(s)	80
ApeKI	GCWGC	4 cut(s)	416, 511, 767, 836
Asp700I	GAANNNNTTC	1 cut(s)	634
AspLEI	GCGC	1 cut(s)	73
AspS9I	GGNCC	5 cut(s)	76, 77, 372, 665, 674
AsuC2I	CCSGG	2 cut(s)	75, 80
AsuHPI	GGTGA	2 cut(s)	794, 875
AvaI	CYCGRG	1 cut(s)	125
AvaII	GGWCC	2 cut(s)	665, 674
AxyI	CCTNAGG	1 cut(s)	503
BaeGI	GKGCMC	2 cut(s)	80, 340
BalI	TGGCCA	2 cut(s)	252, 576
BanI	GGYRCC	1 cut(s)	335
BanII	GRGCYC	3 cut(s)	80, 224, 320
Bbv12I	GWGCWC	1 cut(s)	647
BbvI	GCAGC	4 cut(s)	428, 523, 754, 823
BccI	CCATC	3 cut(s)	95, 676, 827
BceAI	ACGGC	3 cut(s)	148, 196, 831
BcgI	CGANNNNNNTGC	2 cut(s)	50, 84
BciT130I	CCWGG	1 cut(s)	197
BcnI	CCSGG	2 cut(s)	75, 80
BcoDI	GTCTC	2 cut(s)	687, 739
BfmI	CTRYAG	1 cut(s)	837
BisI	GCNGC	7 cut(s)	52, 55, 417, 512, 659, 768, 837
BlsI	GCNGC	7 cut(s)	53, 56, 418, 513, 660, 769, 838
Bme1390I	CCNGG	3 cut(s)	75, 80, 197
Bme18I	GGWCC	2 cut(s)	665, 674
BmeT110I	CYCGRG	1 cut(s)	125
BmgT120I	GGNCC	5 cut(s)	76, 77, 372, 665, 674
BmiI	GGNNCC	3 cut(s)	78, 223, 337
BmrFI	CCNGG	3 cut(s)	75, 80, 197
BmrI	ACTGGG	1 cut(s)	761
BmsI	GCATC	6 cut(s)	58, 428, 446, 498, 645, 832
BmuI	ACTGGG	1 cut(s)	761
BplI	GAGNNNNNCTC	2 cut(s)	143, 175
BpmI	CTGGAG	2 cut(s)	208, 792
BpuMI	CCSGG	2 cut(s)	75, 80
BsaI	GGTCTC	2 cut(s)	687, 739
BsaJI	CCNNGG	3 cut(s)	21, 340, 677
BsaXI	ACNNNNNCTCC	2 cut(s)	206, 236
Bsc4I	CCNNNNNNNGG	4 cut(s)	22, 50, 202, 218
Bse118I	RCCGGY	1 cut(s)	47
Bse1I	ACTGG	3 cut(s)	225, 756, 814
Bse21I	CCTNAGG	1 cut(s)	503
Bse3DI	GCAATG	2 cut(s)	607, 697
BseBI	CCWGG	1 cut(s)	197
BseDI	CCNNGG	3 cut(s)	21, 340, 677
BseGI	GGATG	1 cut(s)	513
BseLI	CCNNNNNNNGG	4 cut(s)	22, 50, 202, 218
BseMI	GCAATG	2 cut(s)	607, 697
BseMII	CTCAG	4 cut(s)	374, 517, 776, 816
BseNI	ACTGG	3 cut(s)	225, 756, 814
BseRI	GAGGAG	1 cut(s)	164
BseSI	GKGCMC	2 cut(s)	80, 340
BseXI	GCAGC	4 cut(s)	428, 523, 754, 823
BseYI	CCCAGC	1 cut(s)	564
BshFI	GGCC	7 cut(s)	78, 117, 155, 252, 374, 576, 818
BshNI	GGYRCC	1 cut(s)	335
BsiHKAI	GWGCWC	1 cut(s)	647
BsiHKCI	CYCGRG	1 cut(s)	125
BsiSI	CCGG	3 cut(s)	48, 74, 80
BslI	CCNNNNNNNGG	4 cut(s)	22, 50, 202, 218
BsmAI	GTCTC	2 cut(s)	687, 739
BsnI	GGCC	7 cut(s)	78, 117, 155, 252, 374, 576, 818
Bso31I	GGTCTC	2 cut(s)	687, 739
BsoBI	CYCGRG	1 cut(s)	125
Bsp120I	GGGCCC	1 cut(s)	76
Bsp1286I	GDGCHC	5 cut(s)	80, 224, 320, 340, 647
Bsp1407I	TGTACA	1 cut(s)	826
Bsp143I	GATC	1 cut(s)	39
Bsp19I	CCATGG	1 cut(s)	21
BspACI	CCGC	6 cut(s)	45, 51, 54, 135, 183, 658
BspANI	GGCC	7 cut(s)	78, 117, 155, 252, 374, 576, 818
BspCNI	CTCAG	4 cut(s)	373, 516, 775, 815
BspLI	GGNNCC	3 cut(s)	78, 223, 337
BspMAI	CTGCAG	1 cut(s)	841
BspPI	GGATC	1 cut(s)	34
BspT107I	GGYRCC	1 cut(s)	335
BspTNI	GGTCTC	2 cut(s)	687, 739
BsrDI	GCAATG	2 cut(s)	607, 697
BsrFI	RCCGGY	1 cut(s)	47
BsrGI	TGTACA	1 cut(s)	826
BsrI	ACTGG	3 cut(s)	225, 756, 814
BssAI	RCCGGY	1 cut(s)	47
BssECI	CCNNGG	3 cut(s)	21, 340, 677
BssMI	GATC	1 cut(s)	39
BssT1I	CCWWGG	3 cut(s)	21, 340, 677
Bst2UI	CCWGG	1 cut(s)	197
Bst4CI	ACNGT	1 cut(s)	309
BstAUI	TGTACA	1 cut(s)	826
BstC8I	GCNNGC	1 cut(s)	49
BstDEI	CTNAG	5 cut(s)	360, 503, 762, 802, 891
BstDSI	CCRYGG	1 cut(s)	21
BstF5I	GGATG	1 cut(s)	513
BstHHI	GCGC	1 cut(s)	73
BstKTI	GATC	1 cut(s)	42
BstMAI	GTCTC	2 cut(s)	687, 739
BstMBI	GATC	1 cut(s)	39
BstMWI	GCNNNNNNNGC	2 cut(s)	842, 881
BstNI	CCWGG	1 cut(s)	197
BstNSI	RCATGY	1 cut(s)	31
BstSCI	CCNGG	3 cut(s)	73, 78, 195
BstSFI	CTRYAG	1 cut(s)	837
BstSLI	GKGCMC	2 cut(s)	80, 340
BstV1I	GCAGC	4 cut(s)	428, 523, 754, 823
BstX2I	RGATCY	1 cut(s)	39
BstYI	RGATCY	1 cut(s)	39
Bsu36I	CCTNAGG	1 cut(s)	503
BsuRI	GGCC	7 cut(s)	78, 117, 155, 252, 374, 576, 818
BtgI	CCRYGG	1 cut(s)	21
BtsCI	GGATG	1 cut(s)	513
BtsIMutI	CAGTG	2 cut(s)	749, 807
Cac8I	GCNNGC	1 cut(s)	49
CaiI	CAGNNNCTG	2 cut(s)	809, 845
CfoI	GCGC	1 cut(s)	73
Cfr10I	RCCGGY	1 cut(s)	47
Cfr13I	GGNCC	5 cut(s)	76, 77, 372, 665, 674
Csp6I	GTAC	2 cut(s)	4, 827
CspCI	CAANNNNNGTGG	2 cut(s)	679, 714
CviAII	CATG	5 cut(s)	22, 28, 202, 352, 615
CviQI	GTAC	2 cut(s)	4, 827
DdeI	CTNAG	5 cut(s)	360, 503, 762, 802, 891
DpnI	GATC	1 cut(s)	41
DpnII	GATC	1 cut(s)	39
DraIII	CACNNNGTG	1 cut(s)	626
DrdI	GACNNNNNNGTC	2 cut(s)	137, 753
DseDI	GACNNNNNNGTC	2 cut(s)	137, 753
EaeI	YGGCCR	3 cut(s)	250, 574, 816
EciI	GGCGGA	1 cut(s)	198
Eco130I	CCWWGG	3 cut(s)	21, 340, 677
Eco24I	GRGCYC	3 cut(s)	80, 224, 320
Eco31I	GGTCTC	2 cut(s)	687, 739
Eco47I	GGWCC	2 cut(s)	665, 674
Eco57I	CTGAAG	1 cut(s)	810
Eco81I	CCTNAGG	1 cut(s)	503
Eco88I	CYCGRG	1 cut(s)	125
EcoRII	CCWGG	1 cut(s)	195
EcoT14I	CCWWGG	3 cut(s)	21, 340, 677
EcoT38I	GRGCYC	3 cut(s)	80, 224, 320
ErhI	CCWWGG	3 cut(s)	21, 340, 677
FaeI	CATG	5 cut(s)	25, 31, 205, 355, 618
FaiI	YATR	8 cut(s)	23, 29, 203, 353, 377, 411, 480, 616
FatI	CATG	5 cut(s)	21, 27, 201, 351, 614
FauI	CCCGC	2 cut(s)	52, 128
FblI	GTMKAC	2 cut(s)	258, 348
Fnu4HI	GCNGC	7 cut(s)	52, 55, 417, 512, 659, 768, 837
FokI	GGATG	1 cut(s)	520
FriOI	GRGCYC	3 cut(s)	80, 224, 320
Fsp4HI	GCNGC	7 cut(s)	52, 55, 417, 512, 659, 768, 837
GlaI	GCGC	1 cut(s)	72
GluI	GCNGC	7 cut(s)	52, 55, 417, 512, 659, 768, 837
GsaI	CCCAGC	1 cut(s)	568
GsuI	CTGGAG	2 cut(s)	208, 792
HaeIII	GGCC	7 cut(s)	78, 117, 155, 252, 374, 576, 818
HapII	CCGG	3 cut(s)	48, 74, 80
HhaI	GCGC	1 cut(s)	73
Hin1II	CATG	5 cut(s)	25, 31, 205, 355, 618
Hin6I	GCGC	1 cut(s)	71
HinP1I	GCGC	1 cut(s)	71
HinfI	GANTC	6 cut(s)	83, 269, 323, 364, 581, 800
HpaII	CCGG	3 cut(s)	48, 74, 80
HphI	GGTGA	2 cut(s)	794, 875
Hpy166II	GTNNAC	4 cut(s)	172, 259, 349, 829
Hpy188I	TCNGA	7 cut(s)	163, 322, 363, 369, 476, 550, 805
Hpy188III	TCNNGA	2 cut(s)	491, 505
Hpy8I	GTNNAC	4 cut(s)	172, 259, 349, 829
Hpy99I	CGWCG	4 cut(s)	65, 68, 146, 149
HpyAV	CCTTC	1 cut(s)	107
HpyCH4III	ACNGT	1 cut(s)	309
HpyCH4IV	ACGT	2 cut(s)	168, 261
HpyCH4V	TGCA	6 cut(s)	31, 511, 605, 618, 690, 839
HpyF10VI	GCNNNNNNNGC	2 cut(s)	842, 881
HpyF3I	CTNAG	5 cut(s)	360, 503, 762, 802, 891
HpySE526I	ACGT	2 cut(s)	168, 261
Hsp92II	CATG	5 cut(s)	25, 31, 205, 355, 618
HspAI	GCGC	1 cut(s)	71
KroI	GCCGGC	1 cut(s)	47
KroNI	GCCGGC	1 cut(s)	49
Kzo9I	GATC	1 cut(s)	39
LmnI	GCTCC	1 cut(s)	227
Lsp1109I	GCAGC	4 cut(s)	428, 523, 754, 823
LweI	GCATC	6 cut(s)	58, 428, 446, 498, 645, 832
MaeII	ACGT	2 cut(s)	168, 261
MaeIII	GTNAC	2 cut(s)	274, 515
MalI	GATC	1 cut(s)	41
MboI	GATC	1 cut(s)	39
MboII	GAAGA	2 cut(s)	321, 528
MflI	RGATCY	1 cut(s)	39
MhlI	GDGCHC	5 cut(s)	80, 224, 320, 340, 647
MlsI	TGGCCA	2 cut(s)	252, 576
MluCI	AATT	1 cut(s)	527
MluNI	TGGCCA	2 cut(s)	252, 576
MlyI	GAGTC	4 cut(s)	92, 263, 590, 794
MmeI	TCCRAC	1 cut(s)	413
Mox20I	TGGCCA	2 cut(s)	252, 576
MreI	CGCCGGCG	1 cut(s)	47
MroNI	GCCGGC	1 cut(s)	47
MroXI	GAANNNNTTC	1 cut(s)	634
MscI	TGGCCA	2 cut(s)	252, 576
MseI	TTAA	1 cut(s)	425
MslI	CAYNNNNRTG	2 cut(s)	26, 650
Msp20I	TGGCCA	2 cut(s)	252, 576
MspA1I	CMGCKG	1 cut(s)	564
MspI	CCGG	3 cut(s)	48, 74, 80
MspR9I	CCNGG	3 cut(s)	75, 80, 197
MvaI	CCWGG	1 cut(s)	197
MwoI	GCNNNNNNNGC	2 cut(s)	842, 881
NaeI	GCCGGC	1 cut(s)	49
NciI	CCSGG	2 cut(s)	75, 80
NcoI	CCATGG	1 cut(s)	21
NdeII	GATC	1 cut(s)	39
NgoMIV	GCCGGC	1 cut(s)	47
NlaIII	CATG	5 cut(s)	25, 31, 205, 355, 618
NlaIV	GGNNCC	3 cut(s)	78, 223, 337
NspI	RCATGY	1 cut(s)	31
PaeR7I	CTCGAG	1 cut(s)	125
PcsI	WCGNNNNNNNCGW	1 cut(s)	138
PdiI	GCCGGC	1 cut(s)	49
PdmI	GAANNNNTTC	1 cut(s)	634
PfeI	GAWTC	2 cut(s)	323, 364
PflMI	CCANNNNNTGG	1 cut(s)	22
PkrI	GCNGC	7 cut(s)	53, 56, 418, 513, 660, 769, 838
PleI	GAGTC	4 cut(s)	91, 263, 589, 794
PpsI	GAGTC	4 cut(s)	91, 263, 589, 794
Psp6I	CCWGG	1 cut(s)	195
PspFI	CCCAGC	1 cut(s)	564
PspGI	CCWGG	1 cut(s)	195
PspN4I	GGNNCC	3 cut(s)	78, 223, 337
PspOMI	GGGCCC	1 cut(s)	76
PspPI	GGNCC	5 cut(s)	76, 77, 372, 665, 674
PspXI	VCTCGAGB	1 cut(s)	125
PstI	CTGCAG	1 cut(s)	841
PstNI	CAGNNNCTG	2 cut(s)	809, 845
PsuI	RGATCY	1 cut(s)	39
PvuII	CAGCTG	1 cut(s)	564
RsaI	GTAC	2 cut(s)	5, 828
RsaNI	GTAC	2 cut(s)	4, 827
RseI	CAYNNNNRTG	2 cut(s)	26, 650
SaqAI	TTAA	1 cut(s)	425
SatI	GCNGC	7 cut(s)	52, 55, 417, 512, 659, 768, 837
Sau3AI	GATC	1 cut(s)	39
Sau96I	GGNCC	5 cut(s)	76, 77, 372, 665, 674
SchI	GAGTC	4 cut(s)	92, 263, 590, 794
ScrFI	CCNGG	3 cut(s)	75, 80, 197
SduI	GDGCHC	5 cut(s)	80, 224, 320, 340, 647
SfaNI	GCATC	6 cut(s)	58, 428, 446, 498, 645, 832
SfcI	CTRYAG	1 cut(s)	837
Sfr274I	CTCGAG	1 cut(s)	125
SgrAI	CRCCGGYG	1 cut(s)	47
SinI	GGWCC	2 cut(s)	665, 674
SlaI	CTCGAG	1 cut(s)	125
SmiMI	CAYNNNNRTG	2 cut(s)	26, 650
SmlI	CTYRAG	1 cut(s)	125
SmoI	CTYRAG	1 cut(s)	125
Sse9I	AATT	1 cut(s)	527
SsiI	CCGC	6 cut(s)	45, 51, 54, 135, 183, 658
StyD4I	CCNGG	3 cut(s)	73, 78, 195
StyI	CCWWGG	3 cut(s)	21, 340, 677
TaaI	ACNGT	1 cut(s)	309
TaiI	ACGT	2 cut(s)	171, 264
TaqI	TCGA	3 cut(s)	90, 126, 147
TasI	AATT	1 cut(s)	527
TatI	WGTACW	1 cut(s)	826
TauI	GCSGC	3 cut(s)	54, 57, 661
TfiI	GAWTC	2 cut(s)	323, 364
Tru1I	TTAA	1 cut(s)	425
Tru9I	TTAA	1 cut(s)	425
TscAI	CASTG	2 cut(s)	756, 814
TseI	GCWGC	4 cut(s)	416, 511, 767, 836
TspGWI	ACGGA	4 cut(s)	21, 262, 476, 567
TspRI	CASTG	2 cut(s)	756, 814
Van91I	CCANNNNNTGG	1 cut(s)	22
VpaK11BI	GGWCC	2 cut(s)	665, 674
XceI	RCATGY	1 cut(s)	31
XcmI	CCANNNNNNNNNTGG	1 cut(s)	748
XhoI	CTCGAG	1 cut(s)	125
XmiI	GTMKAC	2 cut(s)	258, 348
XmnI	GAANNNNTTC	1 cut(s)	634

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.