Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0007570

Physical Location & Seq

Genomic Coordinates Forward (+)

7981918 .. 7985898

Gene Structure 3981 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000006518

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2130 bp

ATGGCAGCCACATCGCCATCTCCGAACTCATCTCCTTCAGCTGTGCCACCAGCTTCTAGTGCTAGTTCCACACCACCACCACAGCCCAATGTGGAATCACCAGATTCCTCAAACACTACAACTCAATCTCCACCACCTGATGTTCCATCAGCTCCTCCTCCAACTGCATCATCACCACCTCCAAATCCATCCTCCCCTTCAGTACCTACACAATCTGATCCACCAGCCTCACCGCCACCGCCTTCTTCATCCAATGTGCCTCCACCATCTTCAAATTCTCCACCCCCACCATCATCTGACCCACCAAAGAGTTCATCTTCCCCACTACCACCAACGTCTGATCCACCTGCAAGTTCACCTCCGCCACCGCAGCCAGTCAGCTCACCTCCCCCACCGCAGCCAGTGAGTTCACCTCCACCACCAACTTCAAGTCCACCTAAGACTTCACCTCCACCTCCATCATCAACGCCACCCAAAAGTTCACCTCCACCTCCATCATCAACACCGCCCAAAAGTTCACCTCCACCACCATCAGAGCCGCCTGAGAAATCTCCTCCACCTCCACCAGCAAATCCCCCTGAAACATCACCACCGCCTCCACCAACATCAAAGCCCCCTGAAACGTCACCACCGCCACCACCAACATTAAAGCCACCTGAGAAATCACCACCACCTCCAGAATCTGAGCCACCCAAAAGTTCACCTCCACCACCAGCATCAACTCCTCCTAAAAGTTCACCTCCTCCACAGGCATCAGAGCCTCCAAGCAGTTCACCCCCACCTTCAAGCCCCAACCCTCCCCCTCCACCCGGTTCTCAACCAACCCCTGTAGCCCCACCTCCGAAGTCATCAGCTCCACCACCCACAATACGACTCTCACCACCTCCACCCTCAGATGATGCTCCACCACCAACTACTCAAATTCCAAGCCCACCATCAAATATTCCAAGTTCAAACAATTCCAATCCTAATGTACCACCAAGTTCAAATGCCACAAGTAATAGTGGCCTCAGCACTGGAGGTGCAGTGGCAATTAGTGTAGTGGCTGGGATAATAGTGCTTAGCTTCATTGCATTCGCTGTATGGTGCATGAGGAGGAAGCCAAAGAAAAAGGTTTCTGGATTTGGAGGTAATGTTATGCCATCGCCGATGGTATCTTCTCCCCAATCAGATTCATCATCATTCTTGAAGGCACATTCTTCAGCTCCCCTTATAGGAAGTGGTTCTGGCAGTGATTTCGTGAATTCTCCTGCAGAACCGGCAGGAGGCAATTCTAGACCTTGGTTTACTTATGACGAACTATTTAAGGCTACAAATGGCTTTTCATCGCAGAATCTTTTGGGGGAAGGTGGATTTGGCTCTGTATATAAGGGAATCCTGCCAGATGATAGAGAGGTGGCTGTAAAACAATTGAAAATAGGTGGGAGTCAGGGTGAGAGAGAATTCAAAGCTGAAGTTGAGACCATTAGTCGCGTTCACCACCGCCATTTGGTTTCTTTGGTGGGCTATTGCATTTCTGAGAACCAAAGGCTGCTTGTCTATGAATATGTCTCTAATGATACCCTCTATTTCCATCTTCATGGAGAAGGTAGGCCAGTTCTTGACTGGGCAACACGTGTTAAAGTTGCTGCTGGTGCAGCTCGTGGAATCGCCTATCTTCATGAAGACTGCCATCCTCGCATTATTCACAGGGATATAAAGTCATCAAACATTCTATTGGATAACAACTTTGAAGCTCGGGTTTCAGATTTTGGACTTGCCAAATTGTGTCTGGATGCAAATACACATATAAGTACACGTGTCATGGGAACTTTTGGATATGTGGCCCCTGAGTATGCTTCAAGTGGCAAACTGACTGAGAAATCTGATGTGTACTCGTTTGGAGTTGTTCTTCTGGAGTTGATTACTGGACGGAAGGCTGTGGATGCATCCCAACCGTTGGGAGATGAGAGTCTAGTTGAATGGGTAAACTTAATGATCAATCTTTTATATCTTCTATTCGCTACCGATTGTTGCATGTTCCCACTATCAATATTGCTTTTACTTGTTCTTAGACAGGAAATTGATATTTCATATCTAATATTTTTGAAGTCTAAATTTAATAAATCATATCTTCATAGCCAAGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

710

Amino Acids

73.65

Weight (kDa)

6.29

Isoelectric Point (pI)

87.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	442 - 642	7e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	444 - 639	2.7e-43	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007570)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26150 AT1G26150 AT1G68690 AT1G68690 AT1G68690 AT1G68690
fragaria_vesca	FvH4_4g28470 FvH4_4g28470 FvH4_4g28470
malus_domestica	MD13G1050900.v1.1 MD16G1051300.v1.1
prunus_persica	Prupe.1G301200_v2.0.a1 Prupe.1G301200_v2.0.a1
pyrus_communis	pycom13g04500 pycom16g04510
rosa_chinensis	RchiOBHm_Chr4g0436441
rosa_laevigata	RLG00000006518
rosa_multiflora	Rmu_sc0000579.1_g000005
rosa_rugosa	Rorug04G0293900
rosa_samantha	Rh4AG348400 Rh4BG357100 Rh4CG371600 Rh4DG350800
rosa_wichuraiana	Rw4G030450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	355
Acc36I	ACCTGC	1 cut(s)	355
AccB7I	CCANNNNNTGG	1 cut(s)	1939
AccII	CGCG	1 cut(s)	1473
AclWI	GGATC	2 cut(s)	212, 335
AcsI	RAATTY	5 cut(s)	274, 921, 1243, 1442, 2096
AcuI	CTGAAG	4 cut(s)	21, 183, 1185, 1473
AcvI	CACGTG	2 cut(s)	1616, 1799
AfaI	GTAC	4 cut(s)	204, 975, 1795, 1874
AfiI	CCNNNNNNNGG	5 cut(s)	809, 1214, 1265, 1489, 1939
AflIII	ACRYGT	4 cut(s)	1613, 1615, 1796, 1798
AhdI	GACNNNNNGTC	1 cut(s)	1467
AjuI	GAANNNNNNNTTGG	2 cut(s)	1338, 1370
Alw26I	GTCTC	2 cut(s)	1454, 1555
AlwI	GGATC	2 cut(s)	212, 335
AlwNI	CAGNNNCTG	1 cut(s)	683
Ama87I	CYCGRG	1 cut(s)	1737
AoxI	GGCC	3 cut(s)	1006, 1592, 1824
ApeKI	GCWGC	6 cut(s)	5, 370, 397, 1531, 1628, 1637
ApoI	RAATTY	5 cut(s)	274, 921, 1243, 1442, 2096
Asp700I	GAANNNNTTC	1 cut(s)	1222
AspS9I	GGNCC	1 cut(s)	1825
AsuC2I	CCSGG	1 cut(s)	810
AvaI	CYCGRG	1 cut(s)	1737
BauI	CACGAG	1 cut(s)	1641
BbrPI	CACGTG	2 cut(s)	1616, 1799
BbsI	GAAGAC	1 cut(s)	1671
BbvCI	CCTCAGC	1 cut(s)	1010
BbvI	GCAGC	6 cut(s)	17, 382, 409, 1518, 1615, 1649
BcgI	CGANNNNNNTGC	3 cut(s)	28, 1219, 1253
BclI	TGATCA	1 cut(s)	1977
BcnI	CCSGG	1 cut(s)	810
BcoDI	GTCTC	2 cut(s)	1454, 1555
BfaI	CTAG	4 cut(s)	57, 63, 1275, 1955
BfmI	CTRYAG	2 cut(s)	828, 1251
BfuAI	ACCTGC	1 cut(s)	355
BisI	GCNGC	7 cut(s)	6, 371, 398, 539, 1532, 1629, 1638
BlpI	GCTNAGC	1 cut(s)	1061
BlsI	GCNGC	7 cut(s)	7, 372, 399, 540, 1533, 1630, 1639
Bme1390I	CCNGG	1 cut(s)	810
BmeRI	GACNNNNNGTC	1 cut(s)	1467
BmeT110I	CYCGRG	1 cut(s)	1737
BmgT120I	GGNCC	1 cut(s)	1825
BmiI	GGNNCC	1 cut(s)	1827
BmrFI	CCNGG	1 cut(s)	810
BmrI	ACTGGG	1 cut(s)	1615
BmsI	GCATC	7 cut(s)	176, 725, 761, 889, 1765, 1915, 1937
BmuI	ACTGGG	1 cut(s)	1615
BpiI	GAAGAC	1 cut(s)	1671
BplI	GAGNNNNNCTC	2 cut(s)	397, 429
BpmI	CTGGAG	3 cut(s)	660, 1038, 1916
Bpu10I	CCTNAGC	1 cut(s)	1010
Bpu1102I	GCTNAGC	1 cut(s)	1061
BpuMI	CCSGG	1 cut(s)	810
BsaAI	YACGTR	2 cut(s)	1616, 1799
BsaI	GGTCTC	1 cut(s)	1454
BsaJI	CCNNGG	2 cut(s)	1280, 2122
Bsc4I	CCNNNNNNNGG	5 cut(s)	809, 1214, 1265, 1489, 1939
Bse118I	RCCGGY	1 cut(s)	1258
Bse1I	ACTGG	6 cut(s)	374, 401, 1021, 1595, 1610, 1912
Bse3DI	GCAATG	1 cut(s)	1068
BseDI	CCNNGG	2 cut(s)	1280, 2122
BseGI	GGATG	6 cut(s)	188, 248, 1672, 1780, 1928, 1930
BseLI	CCNNNNNNNGG	5 cut(s)	809, 1214, 1265, 1489, 1939
BseMI	GCAATG	1 cut(s)	1068
BseMII	CTCAG	8 cut(s)	534, 648, 675, 906, 1024, 1509, 1821, 1848
BseNI	ACTGG	6 cut(s)	374, 401, 1021, 1595, 1610, 1912
BseRI	GAGGAG	6 cut(s)	144, 147, 543, 714, 732, 1108
BseXI	GCAGC	6 cut(s)	17, 382, 409, 1518, 1615, 1649
BseYI	CCCAGC	1 cut(s)	1046
BsgI	GTGCAG	2 cut(s)	1044, 1656
Bsh1236I	CGCG	1 cut(s)	1473
BshFI	GGCC	3 cut(s)	1008, 1594, 1826
BsiHKCI	CYCGRG	1 cut(s)	1737
BsiSI	CCGG	2 cut(s)	810, 1259
BslI	CCNNNNNNNGG	5 cut(s)	809, 1214, 1265, 1489, 1939
BsmAI	GTCTC	2 cut(s)	1454, 1555
BsmI	GAATGC	1 cut(s)	1073
BsnI	GGCC	3 cut(s)	1008, 1594, 1826
Bso31I	GGTCTC	1 cut(s)	1454
BsoBI	CYCGRG	1 cut(s)	1737
Bsp143I	GATC	3 cut(s)	217, 340, 1977
Bsp1720I	GCTNAGC	1 cut(s)	1061
BspANI	GGCC	3 cut(s)	1008, 1594, 1826
BspCNI	CTCAG	8 cut(s)	535, 649, 676, 905, 1023, 1510, 1822, 1849
BspFNI	CGCG	1 cut(s)	1473
BspHI	TCATGA	1 cut(s)	1660
BspLI	GGNNCC	1 cut(s)	1827
BspMAI	CTGCAG	1 cut(s)	1255
BspMI	ACCTGC	1 cut(s)	355
BspPI	GGATC	2 cut(s)	212, 335
BspTNI	GGTCTC	1 cut(s)	1454
BsrDI	GCAATG	1 cut(s)	1068
BsrFI	RCCGGY	1 cut(s)	1258
BsrI	ACTGG	6 cut(s)	374, 401, 1021, 1595, 1610, 1912
BssAI	RCCGGY	1 cut(s)	1258
BssECI	CCNNGG	2 cut(s)	1280, 2122
BssMI	GATC	3 cut(s)	217, 340, 1977
BssSI	CACGAG	1 cut(s)	1641
BssT1I	CCWWGG	2 cut(s)	1280, 2122
Bst2BI	CACGAG	1 cut(s)	1641
Bst4CI	ACNGT	1 cut(s)	1938
BstBAI	YACGTR	2 cut(s)	1616, 1799
BstF5I	GGATG	6 cut(s)	188, 248, 1672, 1780, 1928, 1930
BstFNI	CGCG	1 cut(s)	1473
BstKTI	GATC	3 cut(s)	220, 343, 1980
BstMAI	GTCTC	2 cut(s)	1454, 1555
BstMBI	GATC	3 cut(s)	217, 340, 1977
BstMWI	GCNNNNNNNGC	7 cut(s)	59, 370, 1259, 1634, 1637, 1677, 1925
BstNSI	RCATGY	1 cut(s)	2020
BstSCI	CCNGG	1 cut(s)	808
BstSFI	CTRYAG	2 cut(s)	828, 1251
BstUI	CGCG	1 cut(s)	1473
BstV1I	GCAGC	6 cut(s)	17, 382, 409, 1518, 1615, 1649
BstV2I	GAAGAC	1 cut(s)	1671
BstXI	CCANNNNNNTGG	1 cut(s)	1580
BsuRI	GGCC	3 cut(s)	1008, 1594, 1826
BtgZI	GCGATG	2 cut(s)	1128, 1311
BtsCI	GGATG	6 cut(s)	188, 248, 1672, 1780, 1928, 1930
BtsI	GCAGTG	2 cut(s)	1032, 1237
BtsIMutI	CAGTG	4 cut(s)	408, 1014, 1032, 1237
BveI	ACCTGC	1 cut(s)	355
CaiI	CAGNNNCTG	1 cut(s)	683
CciI	TCATGA	1 cut(s)	1660
Cfr10I	RCCGGY	1 cut(s)	1258
Cfr13I	GGNCC	1 cut(s)	1825
Csp6I	GTAC	4 cut(s)	203, 974, 1794, 1873
CviAII	CATG	5 cut(s)	1090, 1580, 1661, 1804, 2017
CviQI	GTAC	4 cut(s)	203, 974, 1794, 1873
DpnI	GATC	3 cut(s)	219, 342, 1979
DpnII	GATC	3 cut(s)	217, 340, 1977
DriI	GACNNNNNGTC	1 cut(s)	1467
Eam1105I	GACNNNNNGTC	1 cut(s)	1467
EciI	GGCGGA	1 cut(s)	351
Eco130I	CCWWGG	2 cut(s)	1280, 2122
Eco31I	GGTCTC	1 cut(s)	1454
Eco57I	CTGAAG	4 cut(s)	21, 183, 1185, 1473
Eco72I	CACGTG	2 cut(s)	1616, 1799
Eco88I	CYCGRG	1 cut(s)	1737
EcoRI	GAATTC	2 cut(s)	1243, 1442
EcoT14I	CCWWGG	2 cut(s)	1280, 2122
EcoT22I	ATGCAT	1 cut(s)	1930
ErhI	CCWWGG	2 cut(s)	1280, 2122
FaeI	CATG	5 cut(s)	1093, 1583, 1664, 1807, 2020
FatI	CATG	5 cut(s)	1089, 1579, 1660, 1803, 2016
FbaI	TGATCA	1 cut(s)	1977
Fnu4HI	GCNGC	7 cut(s)	6, 371, 398, 539, 1532, 1629, 1638
FokI	GGATG	6 cut(s)	175, 235, 1659, 1787, 1915, 1937
Fsp4HI	GCNGC	7 cut(s)	6, 371, 398, 539, 1532, 1629, 1638
FspBI	CTAG	4 cut(s)	57, 63, 1275, 1955
GluI	GCNGC	7 cut(s)	6, 371, 398, 539, 1532, 1629, 1638
GsaI	CCCAGC	1 cut(s)	1050
GsuI	CTGGAG	3 cut(s)	660, 1038, 1916
HaeIII	GGCC	3 cut(s)	1008, 1594, 1826
HapII	CCGG	2 cut(s)	810, 1259
Hin1II	CATG	5 cut(s)	1093, 1583, 1664, 1807, 2020
HpaII	CCGG	2 cut(s)	810, 1259
Hpy188III	TCNNGA	9 cut(s)	677, 1119, 1186, 1240, 1275, 1601, 1661, 1772, 1895
HpyAV	CCTTC	8 cut(s)	45, 207, 252, 792, 1183, 1340, 1580, 1909
HpyCH4III	ACNGT	1 cut(s)	1938
HpyCH4IV	ACGT	4 cut(s)	335, 623, 1615, 1798
HpyF10VI	GCNNNNNNNGC	7 cut(s)	59, 370, 1259, 1634, 1637, 1677, 1925
HpySE526I	ACGT	4 cut(s)	335, 623, 1615, 1798
Hsp92II	CATG	5 cut(s)	1093, 1583, 1664, 1807, 2020
Ksp22I	TGATCA	1 cut(s)	1977
Kzo9I	GATC	3 cut(s)	217, 340, 1977
LmnI	GCTCC	4 cut(s)	157, 859, 907, 1210
Lsp1109I	GCAGC	6 cut(s)	17, 382, 409, 1518, 1615, 1649
LweI	GCATC	7 cut(s)	176, 725, 761, 889, 1765, 1915, 1937
MaeI	CTAG	4 cut(s)	57, 63, 1275, 1955
MaeII	ACGT	4 cut(s)	335, 623, 1615, 1798
MaeIII	GTNAC	1 cut(s)	624
MalI	GATC	3 cut(s)	219, 342, 1979
MboI	GATC	3 cut(s)	217, 340, 1977
MfeI	CAATTG	1 cut(s)	1409
MlyI	GAGTC	3 cut(s)	867, 1435, 1960
MmeI	TCCRAC	1 cut(s)	185
Mph1103I	ATGCAT	1 cut(s)	1930
MroXI	GAANNNNTTC	1 cut(s)	1222
MseI	TTAA	5 cut(s)	647, 1305, 1620, 1973, 2100
MslI	CAYNNNNRTG	1 cut(s)	1578
MspA1I	CMGCKG	1 cut(s)	41
MspI	CCGG	2 cut(s)	810, 1259
MspR9I	CCNGG	1 cut(s)	810
MunI	CAATTG	1 cut(s)	1409
Mva1269I	GAATGC	1 cut(s)	1073
MvnI	CGCG	1 cut(s)	1473
MwoI	GCNNNNNNNGC	7 cut(s)	59, 370, 1259, 1634, 1637, 1677, 1925
NciI	CCSGG	1 cut(s)	810
NdeII	GATC	3 cut(s)	217, 340, 1977
NlaIII	CATG	5 cut(s)	1093, 1583, 1664, 1807, 2020
NlaIV	GGNNCC	1 cut(s)	1827
NmuCI	GTSAC	1 cut(s)	624
NsiI	ATGCAT	1 cut(s)	1930
NspI	RCATGY	1 cut(s)	2020
PagI	TCATGA	1 cut(s)	1660
PaqCI	CACCTGC	1 cut(s)	355
PcsI	WCGNNNNNNNCGW	1 cut(s)	20
PctI	GAATGC	1 cut(s)	1073
PdmI	GAANNNNTTC	1 cut(s)	1222
PfeI	GAWTC	7 cut(s)	95, 104, 680, 1172, 1333, 1374, 1647
PflMI	CCANNNNNTGG	1 cut(s)	1939
PkrI	GCNGC	7 cut(s)	7, 372, 399, 540, 1533, 1630, 1639
PleI	GAGTC	3 cut(s)	867, 1434, 1959
PmaCI	CACGTG	2 cut(s)	1616, 1799
PmlI	CACGTG	2 cut(s)	1616, 1799
PpsI	GAGTC	3 cut(s)	867, 1434, 1959
Ppu21I	YACGTR	2 cut(s)	1616, 1799
PspCI	CACGTG	2 cut(s)	1616, 1799
PspFI	CCCAGC	1 cut(s)	1046
PspN4I	GGNNCC	1 cut(s)	1827
PspPI	GGNCC	1 cut(s)	1825
PstI	CTGCAG	1 cut(s)	1255
PstNI	CAGNNNCTG	1 cut(s)	683
PvuII	CAGCTG	1 cut(s)	41
RsaI	GTAC	4 cut(s)	204, 975, 1795, 1874
RsaNI	GTAC	4 cut(s)	203, 974, 1794, 1873
RseI	CAYNNNNRTG	1 cut(s)	1578
SaqAI	TTAA	5 cut(s)	647, 1305, 1620, 1973, 2100
SatI	GCNGC	7 cut(s)	6, 371, 398, 539, 1532, 1629, 1638
Sau3AI	GATC	3 cut(s)	217, 340, 1977
Sau96I	GGNCC	1 cut(s)	1825
SchI	GAGTC	3 cut(s)	867, 1435, 1960
ScrFI	CCNGG	1 cut(s)	810
SfaNI	GCATC	7 cut(s)	176, 725, 761, 889, 1765, 1915, 1937
SfcI	CTRYAG	2 cut(s)	828, 1251
SmiMI	CAYNNNNRTG	1 cut(s)	1578
SspI	AATATT	3 cut(s)	943, 2034, 2082
SspMI	CTAG	4 cut(s)	57, 63, 1275, 1955
StyD4I	CCNGG	1 cut(s)	808
StyI	CCWWGG	2 cut(s)	1280, 2122
TaaI	ACNGT	1 cut(s)	1938
TaiI	ACGT	4 cut(s)	338, 626, 1618, 1801
TatI	WGTACW	2 cut(s)	1793, 1872
TauI	GCSGC	1 cut(s)	541
TfiI	GAWTC	7 cut(s)	95, 104, 680, 1172, 1333, 1374, 1647
Tru1I	TTAA	5 cut(s)	647, 1305, 1620, 1973, 2100
Tru9I	TTAA	5 cut(s)	647, 1305, 1620, 1973, 2100
TscAI	CASTG	4 cut(s)	408, 1021, 1032, 1237
TseFI	GTSAC	1 cut(s)	624
TseI	GCWGC	6 cut(s)	5, 370, 397, 1531, 1628, 1637
Tsp45I	GTSAC	1 cut(s)	624
TspGWI	ACGGA	1 cut(s)	1927
TspRI	CASTG	4 cut(s)	408, 1021, 1032, 1237
Van91I	CCANNNNNTGG	1 cut(s)	1939
XapI	RAATTY	5 cut(s)	274, 921, 1243, 1442, 2096
XbaI	TCTAGA	1 cut(s)	1274
XceI	RCATGY	1 cut(s)	2020
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1602, 1768
XmnI	GAANNNNTTC	1 cut(s)	1222
XspI	CTAG	4 cut(s)	57, 63, 1275, 1955
Zsp2I	ATGCAT	1 cut(s)	1930

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.