RLG00000007353

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014038
Physical Location & Seq
Reverse (-)
16090491 .. 16100740
10250 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007353

Sequence Viewer

Length: 3324 bp
ATGGCTGCAAATTCACTTCCAATACTATATGCTCTTGTTCTCGCACTGAGCCTGTCCGCGTTTCAATCCGAGTCGGCGCCTCAAGCTTTCCGGAGAGATCCAGGTCACCCTCAGTGGCACCACGGCGCCTTCCACGACGTCCGTGACACCATTCGCTCCGATGTCCGCCGCATGCTCCATTCTCGCGCCGAGGTGCCGTTCCAGGTTCCACTGGAAGTGAATGTGGTGCTTGTTGGTTTCAATGGTGATGGAGGCTATAGGTACTCGGTGGATGCGCACAAATTGGAGGAGCTGCTCAAGATCAGCTTCCCAACACACCGACCGGCGTGTCTTGAGACCGGGGAGGCTCTCGATATCGAGCATCAAATTGTCTACAATGCCTTCCCTGCTGGGCAGCCGGAGCTTATAGCTCTCGAGAAGGCTTTGAAAGAGGCGATGGTTCCTGCAGGAAATGCGAGAGAGACTGAATTTGGAAGGGAAGTACCTCTTTTTGAGGTGGATGCAACTGTAGTGGAGCCGGTATTTCAGAGGTTGTATTCCTATATATTTGACACAGACAATTCCGCTGTTTCTGCTATGGAGTTGGATCGCCAAGTGCCAAGTGCAATATTTGTGGTCAATTTTGATAAGGTCAGAATGGATCCTAGGAATAAGGAAATTGATCTTGACAACTTGATGTATGGAAAAATTACACAACTAACTGATGATGATATGCAAAAACAAGAGGGAGACTATATTTATCGCTATCGGTACAATGGAGGAGGTGCCTCTCAAGTTTGGCTGGGCTATGGCAGATATGTAGTGATAGATCTCTCGGCCGGCCCGTGTACTTATGGAAAGATCGAAACTGAAGAGGGAACTGTCAGTTCTCGGACCCTGCCACGACTACGGAATATTATGTTTCCAAGAGGAATGGGTGCACCTAGTGAACAATCTACACATGATGTATTTGTTGGACAACTTGCCTCTCTAGTATCCACCACGATTGAGCATGTTATAGCTCCAGATGTTAGATTTGAAACTGTTGATCTGACAACAAGGCTGCTTTTACCAATAATTGTGCTGCAAAATCATAATCGGTACAATATTATAGAGAAGGGCCACAACTACAGTATAAATATTGAAGCAATTGAGGAACAGGTGAAGAAAATGGTTCATGATGGGCAAGAAGTTGTGATTGTTGGGGGTTCACATTCATTACATCGCCATGAGAAGTTGGCAATTGCCGTTTCAAAAGCTATGCGAGGCCATTCCCTTCAAGAAACTAGGAATGATGGACGCTTTCATGTTCATACCAAGACATATCTGGATGGTGCTCTTCTTAAAGAAGAGATGGAACGTTCTGCTGATGTGCTTGCTGCTGGTTTGCTTGAGGTGGCTGACCCATCTCTTTCGAGTAAATTTTTCCTCCGCCAGCATTGGAATGATGAATCTGATGGTGCAAGTGATTCTATACTCAAACATAAACCTCTTTGGTCTACTTATGACAAAAAAGGTGGCAAGAAGAAAAAGAAAAAAGAAAGGAAACAGGGAGATCTGTACCGAACTTATGGAACAAGAGTAATTCCTGTCTTTGTGCTATCATTGGCTGATGTGGACCCACACCTTATGATGGAAGACGAAAGTCTTGTATGGACAAGCAAAGATGTCGTGATTGTACTTGAGCATCAAAATCAGCAGATACCTCTTAGTTATGTGTCAGAAACACAGAGAAGGCATGCCGAACCATCTCAGGTACAGCGCCATATATTGGCAGGGCTTGCTTCTGCTGTGGCAGGGTTGAGTGCACCATATGAGAAGGCTTCTCATGTACATGAAAGGCCAGTCGTGAATTGGCTATGGGCAGCTGGGTGTCATCCATTTGGACCATTCTCTAATACTTCTAAAGTCAGCCAAATGCTTCAAGATGTTGCATTGAGGAACTCAATATATGCGCGTGTAGATTCTGCACTCCACAAAATCCGTGATACGTCAGAGGCTGTACAAACATTTGCTGCCGAGTATCTGAAAACTCCGCTTGGTGAGCCAGTGAAGGGCAAGAAGAACAAAACCACCACTGAGCTATGGGTGGAGAAGTTTTACAAGAAGACCACTAACTTGCCTGAACCTTTCCCACATGAATTAGTTGATAGATTGGAGAACTTCTTAAATAACCTTGAGGAACAGCTAGTAGATTTGTCTTCATCATTGTATGGTCACCGGCTACAAGATGCACATTTCAATAGTTCTGAGATTCTCCAGAGTTCCATATTTACCCAGCAGTATGTAGATCATGTTTTGTCAAACGAGAGGGAAAAGATGAAATGCTGCAACATTGAGTACAAATATCCTGTGCAGTCATCTCAAACTTATGTCTATGGGGGGATTCTTCTTGCTGGATTCGTTAAGCAACAGGCATTGCAATCTCTTCATAATGTTATCACCTCAAAGATGTCTAGAGGATGGCAAAAACCACTCGAAAGGTTTATGTTTAAATATGTAGAGCTTTGCGTTGAGTTGCGCAAGGTTATTTTTGCCAAGGATGGTCTGATTAAGTACCGCAATATATGTCAACAAGTAAATGTCAGCTTCTTGGAAGAGGTCATCAATCTGAGCAGGCTGGGAAAGGACCGCAAAGTGGCTACTCCTTGGTTCAAGTTTCTGTGGGAATCCTACAGAACGGTGCTGGACATCTTATGGAACAACTCGAAGTTCGATGCTCTTTATGCGCCTTCCAGTTCTGCAAGAAATACAAACGAACAAGAGTTTCGCAGGTTATGTGGAACCATCAGGAAGCATTTGGCAAATCTGAACAAAGATCAAAGAAACCAGGCTGGCCTATCTTCACCTGAGAGCTTACAAGAAGCATTCCCTTGTATTGAAGATACTCAAAGATTGATGTGCATGGTTAAGAAAACCCCTAAAGCATTGATAGTGGTTGACTATGCCAAGCTAGCAGAATTCTTTGGGGTTTCTGATAGCCACCTCGATCAATCACGCCTACGCATTGCTCAAGCTGTTCACAGTCGATTAGAAAGGGTAGTCCTCGGTGGTCACGACCTATCTTCAGTCAATTACACCTTCGAAGTCCAAGCCATGGGCGCCGAGAAGCTTCCTTTCGTCCTCCCCACCGTCTTCACCATCAGTCCACACGCCGACGACCTGGAGGCCCTCCAGAAGTACGCCAAGCTCATTTCCAACCACGACAAGAACTCCAACCATGTCAAGGAGCTCGTCCATGGCATCATCGAGGGCGAGATACGGGTGCTCGCAGCCTCGATGACGATGGAGGAGGTCTTCAAAGGGACCAAAGAGTTCAAGAAGGAGGTGTTTGAGAAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1108

Amino Acids

125.61

Weight (kDa)

6.63

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7906 PF25483 69 - 335 6.7e-57 Domain of unknown function (DUF7906)
eIF3a_PCI_TPR-like PF22591 796 - 862 9.7e-16 eIF3a, PCI domain, TPR-like region
eIF3a_PCI_TPR-like PF22591 868 - 947 7.5e-18 eIF3a, PCI domain, TPR-like region
Band_7 PF01145 1013 - 1106 6.8e-06 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014038)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16180
fragaria_vesca FvH4_4g19930 FvH4_4g19930
malus_domestica MD16G1152500.v1.1
prunus_persica Prupe.1G115700_v2.0.a1 Prupe.1G115700_v2.0.a1
pyrus_communis pycom16g12970
rosa_chinensis RchiOBHm_Chr4g0425571
rosa_laevigata RLG00000007353
rosa_multiflora Rmu_sc0001900.1_g000001 Rmu_sc0003958.1_g000018
rosa_roxburghii Rroxscaffold_5G00367540
rosa_rugosa Rorug04G0204200
rosa_samantha Rh4AG261100 Rh4BG267000 Rh4CG278200
rosa_wichuraiana Rw4G022610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 327
AatII GACGTC 1 cut(s) 141
Acc16I TGCGCA 2 cut(s) 276, 2501
Acc36I ACCTGC 1 cut(s) 2742
AccB1I GGYRCC 6 cut(s) 76, 117, 125, 193, 764, 3080
AccB7I CCANNNNNTGG 2 cut(s) 1750, 3076
AccI GTMKAC 2 cut(s) 372, 1478
AccII CGCG 3 cut(s) 59, 186, 1936
AccIII TCCGGA 1 cut(s) 90
AciI CCGC 8 cut(s) 57, 166, 169, 564, 1411, 2015, 2539, 2611
AclI AACGTT 1 cut(s) 1339
AclWI GGATC 4 cut(s) 92, 594, 635, 648
AcoI YGGCCR 1 cut(s) 816
AcsI RAATTY 4 cut(s) 10, 467, 1400, 2939
AcuI CTGAAG 2 cut(s) 870, 3030
AcyI GRCGYC 4 cut(s) 77, 126, 138, 3081
AdeI CACNNNGTG 1 cut(s) 926
AfiI CCNNNNNNNGG 6 cut(s) 1612, 1750, 2033, 2617, 3076, 3205
AjnI CCWGG 4 cut(s) 100, 201, 2808, 3141
Alw21I GWGCWC 5 cut(s) 922, 1318, 1789, 3213, 3249
Alw26I GTCTC 3 cut(s) 329, 455, 723
Alw44I GTGCAC 2 cut(s) 918, 1785
AlwI GGATC 4 cut(s) 92, 594, 635, 648
AlwNI CAGNNNCTG 1 cut(s) 1979
Ama87I CYCGRG 1 cut(s) 413
Aor13HI TCCGGA 1 cut(s) 90
AoxI GGCC 7 cut(s) 816, 820, 1099, 1246, 1820, 2815, 3147
ApaLI GTGCAC 2 cut(s) 918, 1785
ApoI RAATTY 4 cut(s) 10, 467, 1400, 2939
AspA2I CCTAGG 1 cut(s) 644
AspLEI GCGC 9 cut(s) 79, 128, 188, 277, 1743, 1936, 2502, 2710, 3083
AspS9I GGNCC 8 cut(s) 821, 873, 1099, 1597, 1865, 2608, 3148, 3285
AsuC2I CCSGG 1 cut(s) 340
AsuHPI GGTGA 8 cut(s) 98, 257, 1153, 2033, 2189, 2413, 2817, 3109
AsuII TTCGAA 1 cut(s) 3063
AsuNHI GCTAGC 1 cut(s) 2932
AvaI CYCGRG 1 cut(s) 413
AvaII GGWCC 5 cut(s) 873, 1597, 1865, 2608, 3285
AvrII CCTAGG 1 cut(s) 644
BaeGI GKGCMC 2 cut(s) 922, 1789
BamHI GGATCC 1 cut(s) 640
BanI GGYRCC 6 cut(s) 76, 117, 125, 193, 764, 3080
BanII GRGCYC 1 cut(s) 3213
BbsI GAAGAC 5 cut(s) 1623, 2093, 2172, 3106, 3268
Bbv12I GWGCWC 5 cut(s) 922, 1318, 1789, 3213, 3249
BbvI GCAGC 9 cut(s) 279, 406, 1029, 1050, 1345, 1856, 1981, 2294, 3263
BceAI ACGGC 3 cut(s) 139, 181, 1211
BcgI CGANNNNNNTGC 2 cut(s) 1630, 1664
BciT130I CCWGG 4 cut(s) 102, 203, 2810, 3143
BciVI GTATCC 1 cut(s) 985
BcnI CCSGG 1 cut(s) 340
BcoDI GTCTC 3 cut(s) 329, 455, 723
BfaI CTAG 7 cut(s) 645, 924, 971, 1266, 2168, 2436, 2933
BfmI CTRYAG 5 cut(s) 256, 444, 507, 1108, 2653
BfoI RGCGCY 4 cut(s) 80, 129, 1744, 3084
BfuAI ACCTGC 1 cut(s) 2742
BfuI GTATCC 1 cut(s) 985
BglII AGATCT 2 cut(s) 808, 1534
BlnI CCTAGG 1 cut(s) 644
Bme1390I CCNGG 5 cut(s) 102, 203, 340, 2810, 3143
Bme18I GGWCC 5 cut(s) 873, 1597, 1865, 2608, 3285
BmeT110I CYCGRG 1 cut(s) 413
BmgT120I GGNCC 8 cut(s) 821, 873, 1099, 1597, 1865, 2608, 3148, 3285
BmrFI CCNGG 5 cut(s) 102, 203, 340, 2810, 3143
BmsI GCATC 7 cut(s) 262, 370, 490, 1675, 2200, 2686, 3231
BmtI GCTAGC 1 cut(s) 2936
BoxI GACNNNNGTC 1 cut(s) 1623
BpiI GAAGAC 5 cut(s) 1623, 2093, 2172, 3106, 3268
BpmI CTGGAG 4 cut(s) 987, 2222, 3137, 3164
Bpu14I TTCGAA 1 cut(s) 3063
BpuEI CTTGAG 8 cut(s) 66, 281, 353, 756, 1391, 1682, 2177, 2976
BpuMI CCSGG 1 cut(s) 340
BsaHI GRCGYC 4 cut(s) 77, 126, 138, 3081
BsaI GGTCTC 1 cut(s) 329
BsaJI CCNNGG 9 cut(s) 121, 189, 339, 644, 2517, 2627, 3025, 3075, 3217
BsaWI WCCGGW 1 cut(s) 90
BsaXI ACNNNNNCTCC 2 cut(s) 3176, 3206
Bsc4I CCNNNNNNNGG 6 cut(s) 1612, 1750, 2033, 2617, 3076, 3205
Bse118I RCCGGY 4 cut(s) 322, 517, 818, 2199
Bse1I ACTGG 4 cut(s) 216, 1823, 2027, 2715
Bse3DI GCAATG 2 cut(s) 2396, 2985
BseAI TCCGGA 1 cut(s) 90
BseBI CCWGG 4 cut(s) 102, 203, 2810, 3143
BseDI CCNNGG 9 cut(s) 121, 189, 339, 644, 2517, 2627, 3025, 3075, 3217
BseGI GGATG 6 cut(s) 277, 505, 1315, 1855, 2447, 2527
BseLI CCNNNNNNNGG 6 cut(s) 1612, 1750, 2033, 2617, 3076, 3205
BseMI GCAATG 2 cut(s) 2396, 2985
BseMII CTCAG 7 cut(s) 38, 125, 1745, 2049, 2220, 2582, 2820
BseNI ACTGG 4 cut(s) 216, 1823, 2027, 2715
BseRI GAGGAG 3 cut(s) 302, 774, 3284
BseSI GKGCMC 2 cut(s) 922, 1789
BseX3I CGGCCG 1 cut(s) 816
BseXI GCAGC 9 cut(s) 279, 406, 1029, 1050, 1345, 1856, 1981, 2294, 3263
BseYI CCCAGC 5 cut(s) 389, 781, 1847, 2256, 2599
BsgI GTGCAG 2 cut(s) 1932, 2354
Bsh1236I CGCG 3 cut(s) 59, 186, 1936
Bsh1285I CGRYCG 2 cut(s) 323, 819
BshFI GGCC 7 cut(s) 818, 822, 1101, 1248, 1822, 2817, 3149
BshNI GGYRCC 6 cut(s) 76, 117, 125, 193, 764, 3080
BsiEI CGRYCG 2 cut(s) 323, 819
BsiHKAI GWGCWC 5 cut(s) 922, 1318, 1789, 3213, 3249
BsiHKCI CYCGRG 1 cut(s) 413
BsiSI CCGG 7 cut(s) 91, 323, 339, 398, 518, 819, 2200
BslFI GGGAC 1 cut(s) 3298
BslI CCNNNNNNNGG 6 cut(s) 1612, 1750, 2033, 2617, 3076, 3205
BsmAI GTCTC 3 cut(s) 329, 455, 723
BsmFI GGGAC 1 cut(s) 3298
BsmI GAATGC 1 cut(s) 2846
BsnI GGCC 7 cut(s) 818, 822, 1101, 1248, 1822, 2817, 3149
Bso31I GGTCTC 1 cut(s) 329
BsoBI CYCGRG 1 cut(s) 413
Bsp119I TTCGAA 1 cut(s) 3063
Bsp1286I GDGCHC 5 cut(s) 922, 1318, 1789, 3213, 3249
Bsp13I TCCGGA 1 cut(s) 90
Bsp1407I TGTACA 2 cut(s) 1810, 1981
Bsp19I CCATGG 2 cut(s) 3075, 3217
BspACI CCGC 8 cut(s) 57, 166, 169, 564, 1411, 2015, 2539, 2611
BspANI GGCC 7 cut(s) 818, 822, 1101, 1248, 1822, 2817, 3149
BspCNI CTCAG 7 cut(s) 39, 124, 1744, 2050, 2221, 2583, 2821
BspEI TCCGGA 1 cut(s) 90
BspFNI CGCG 3 cut(s) 59, 186, 1936
BspHI TCATGA 1 cut(s) 1156
BspMAI CTGCAG 1 cut(s) 448
BspMI ACCTGC 1 cut(s) 2742
BspOI GCTAGC 1 cut(s) 2936
BspPI GGATC 4 cut(s) 92, 594, 635, 648
BspQI GCTCTTC 1 cut(s) 1323
BspT104I TTCGAA 1 cut(s) 3063
BspT107I GGYRCC 6 cut(s) 76, 117, 125, 193, 764, 3080
BspTNI GGTCTC 1 cut(s) 329
BsrDI GCAATG 2 cut(s) 2396, 2985
BsrFI RCCGGY 4 cut(s) 322, 517, 818, 2199
BsrGI TGTACA 2 cut(s) 1810, 1981
BsrI ACTGG 4 cut(s) 216, 1823, 2027, 2715
BssAI RCCGGY 4 cut(s) 322, 517, 818, 2199
BssECI CCNNGG 9 cut(s) 121, 189, 339, 644, 2517, 2627, 3025, 3075, 3217
BssNI GRCGYC 4 cut(s) 77, 126, 138, 3081
BssT1I CCWWGG 5 cut(s) 644, 2517, 2627, 3075, 3217
Bst2UI CCWGG 4 cut(s) 102, 203, 2810, 3143
Bst4CI ACNGT 7 cut(s) 508, 862, 1024, 1112, 2662, 3005, 3112
Bst6I CTCTTC 5 cut(s) 846, 1323, 1323, 2412, 2571
BstACI GRCGYC 4 cut(s) 77, 126, 138, 3081
BstAPI GCANNNNNTGC 2 cut(s) 452, 1760
BstAUI TGTACA 2 cut(s) 1810, 1981
BstBI TTCGAA 1 cut(s) 3063
BstDEI CTNAG 8 cut(s) 47, 111, 1688, 1731, 2058, 2229, 2591, 2829
BstDSI CCRYGG 3 cut(s) 121, 3075, 3217
BstEII GGTNACC 2 cut(s) 104, 2195
BstF5I GGATG 6 cut(s) 277, 505, 1315, 1855, 2447, 2527
BstFNI CGCG 3 cut(s) 59, 186, 1936
BstH2I RGCGCY 4 cut(s) 80, 129, 1744, 3084
BstHHI GCGC 9 cut(s) 79, 128, 188, 277, 1743, 1936, 2502, 2710, 3083
BstMAI GTCTC 3 cut(s) 329, 455, 723
BstMCI CGRYCG 2 cut(s) 323, 819
BstNI CCWGG 4 cut(s) 102, 203, 2810, 3143
BstNSI RCATGY 3 cut(s) 175, 995, 1721
BstPAI GACNNNNGTC 1 cut(s) 1623
BstPI GGTNACC 2 cut(s) 104, 2195
BstSCI CCNGG 5 cut(s) 100, 201, 338, 2808, 3141
BstSFI CTRYAG 5 cut(s) 256, 444, 507, 1108, 2653
BstSLI GKGCMC 2 cut(s) 922, 1789
BstUI CGCG 3 cut(s) 59, 186, 1936
BstV1I GCAGC 9 cut(s) 279, 406, 1029, 1050, 1345, 1856, 1981, 2294, 3263
BstV2I GAAGAC 5 cut(s) 1623, 2093, 2172, 3106, 3268
BstX2I RGATCY 4 cut(s) 97, 640, 808, 1534
BstYI RGATCY 4 cut(s) 97, 640, 808, 1534
BstZI CGGCCG 1 cut(s) 816
BsuI GTATCC 1 cut(s) 985
BsuRI GGCC 7 cut(s) 818, 822, 1101, 1248, 1822, 2817, 3149
BtgI CCRYGG 3 cut(s) 121, 3075, 3217
BtgZI GCGATG 2 cut(s) 449, 1187
BtsCI GGATG 6 cut(s) 277, 505, 1315, 1855, 2447, 2527
BtsIMutI CAGTG 5 cut(s) 44, 119, 209, 2034, 2055
BveI ACCTGC 1 cut(s) 2742
CaiI CAGNNNCTG 1 cut(s) 1979
CciI TCATGA 1 cut(s) 1156
CfoI GCGC 9 cut(s) 79, 128, 188, 277, 1743, 1936, 2502, 2710, 3083
Cfr10I RCCGGY 4 cut(s) 322, 517, 818, 2199
Cfr13I GGNCC 8 cut(s) 821, 873, 1099, 1597, 1865, 2608, 3148, 3285
CseI GACGC 1 cut(s) 1287
CspCI CAANNNNNGTGG 8 cut(s) 492, 527, 594, 629, 967, 1002, 1477, 1512
DdeI CTNAG 8 cut(s) 47, 111, 1688, 1731, 2058, 2229, 2591, 2829
DinI GGCGCC 3 cut(s) 78, 127, 3082
DraI TTTAAA 1 cut(s) 2473
DraIII CACNNNGTG 1 cut(s) 926
DrdI GACNNNNNNGTC 1 cut(s) 327
DseDI GACNNNNNNGTC 1 cut(s) 327
EaeI YGGCCR 1 cut(s) 816
EagI CGGCCG 1 cut(s) 816
Eam1104I CTCTTC 5 cut(s) 846, 1323, 1323, 2412, 2571
EarI CTCTTC 5 cut(s) 846, 1323, 1323, 2412, 2571
EciI GGCGGA 2 cut(s) 155, 1400
Ecl136II GAGCTC 1 cut(s) 3211
EclXI CGGCCG 1 cut(s) 816
Eco130I CCWWGG 5 cut(s) 644, 2517, 2627, 3075, 3217
Eco24I GRGCYC 1 cut(s) 3213
Eco31I GGTCTC 1 cut(s) 329
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 5 cut(s) 873, 1597, 1865, 2608, 3285
Eco52I CGGCCG 1 cut(s) 816
Eco53kI GAGCTC 1 cut(s) 3211
Eco57I CTGAAG 2 cut(s) 870, 3030
Eco88I CYCGRG 1 cut(s) 413
Eco91I GGTNACC 2 cut(s) 104, 2195
EcoICRI GAGCTC 1 cut(s) 3211
EcoO109I RGGNCCY 1 cut(s) 3148
EcoO65I GGTNACC 2 cut(s) 104, 2195
EcoRI GAATTC 1 cut(s) 2939
EcoRII CCWGG 4 cut(s) 100, 201, 2808, 3141
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 5 cut(s) 644, 2517, 2627, 3075, 3217
EcoT38I GRGCYC 1 cut(s) 3213
EgeI GGCGCC 3 cut(s) 78, 127, 3082
EheI GGCGCC 3 cut(s) 78, 127, 3082
ErhI CCWWGG 5 cut(s) 644, 2517, 2627, 3075, 3217
FalI AAGNNNNNCTT 4 cut(s) 290, 322, 471, 503
FaqI GGGAC 1 cut(s) 3298
FauNDI CATATG 1 cut(s) 1792
FblI GTMKAC 2 cut(s) 372, 1478
FokI GGATG 6 cut(s) 284, 512, 1322, 1842, 2454, 2534
FriOI GRGCYC 1 cut(s) 3213
FseI GGCCGGCC 1 cut(s) 822
FspAI RTGCGCAY 1 cut(s) 276
FspBI CTAG 7 cut(s) 645, 924, 971, 1266, 2168, 2436, 2933
FspI TGCGCA 2 cut(s) 276, 2501
GlaI GCGC 9 cut(s) 78, 127, 187, 276, 1742, 1935, 2501, 2709, 3082
GsaI CCCAGC 5 cut(s) 393, 785, 1851, 2260, 2603
GsuI CTGGAG 4 cut(s) 987, 2222, 3137, 3164
HaeII RGCGCY 4 cut(s) 80, 129, 1744, 3084
HaeIII GGCC 7 cut(s) 818, 822, 1101, 1248, 1822, 2817, 3149
HapII CCGG 7 cut(s) 91, 323, 339, 398, 518, 819, 2200
HgaI GACGC 1 cut(s) 1287
HhaI GCGC 9 cut(s) 79, 128, 188, 277, 1743, 1936, 2502, 2710, 3083
Hin1I GRCGYC 4 cut(s) 77, 126, 138, 3081
Hin6I GCGC 9 cut(s) 77, 126, 186, 275, 1741, 1934, 2500, 2708, 3081
HinP1I GCGC 9 cut(s) 77, 126, 186, 275, 1741, 1934, 2500, 2708, 3081
HincII GTYRAC 2 cut(s) 2552, 2920
HindII GTYRAC 2 cut(s) 2552, 2920
HindIII AAGCTT 2 cut(s) 84, 3089
HinfI GANTC 8 cut(s) 71, 1430, 1448, 1943, 2233, 2365, 2379, 2648
HpaII CCGG 7 cut(s) 91, 323, 339, 398, 518, 819, 2200
HphI GGTGA 8 cut(s) 98, 257, 1153, 2033, 2189, 2413, 2817, 3109
Hpy99I CGWCG 2 cut(s) 140, 3140
HpyCH4III ACNGT 7 cut(s) 508, 862, 1024, 1112, 2662, 3005, 3112
HpyCH4IV ACGT 3 cut(s) 138, 1339, 1970
HpyF3I CTNAG 8 cut(s) 47, 111, 1688, 1731, 2058, 2229, 2591, 2829
HpySE526I ACGT 3 cut(s) 138, 1339, 1970
Hsp92I GRCGYC 4 cut(s) 77, 126, 138, 3081
HspAI GCGC 9 cut(s) 77, 126, 186, 275, 1741, 1934, 2500, 2708, 3081
KasI GGCGCC 3 cut(s) 76, 125, 3080
Kpn2I TCCGGA 1 cut(s) 90
KroI GCCGGC 1 cut(s) 818
KroNI GCCGGC 1 cut(s) 820
LguI GCTCTTC 1 cut(s) 1323
LmnI GCTCC 7 cut(s) 161, 180, 289, 400, 514, 1006, 3208
Lsp1109I GCAGC 9 cut(s) 279, 406, 1029, 1050, 1345, 1856, 1981, 2294, 3263
LweI GCATC 7 cut(s) 262, 370, 490, 1675, 2200, 2686, 3231
MaeI CTAG 7 cut(s) 645, 924, 971, 1266, 2168, 2436, 2933
MaeII ACGT 3 cut(s) 138, 1339, 1970
MaeIII GTNAC 4 cut(s) 104, 143, 2195, 3032
MfeI CAATTG 2 cut(s) 1128, 1221
MflI RGATCY 4 cut(s) 97, 640, 808, 1534
MhlI GDGCHC 5 cut(s) 922, 1318, 1789, 3213, 3249
Mly113I GGCGCC 3 cut(s) 77, 126, 3081
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 4 cut(s) 564, 934, 3201, 3219
MroI TCCGGA 1 cut(s) 90
MroNI GCCGGC 1 cut(s) 818
MseI TTAA 6 cut(s) 1323, 2147, 2385, 2472, 2532, 2889
MslI CAYNNNNRTG 3 cut(s) 1206, 1422, 1812
MspA1I CMGCKG 2 cut(s) 566, 1847
MspI CCGG 7 cut(s) 91, 323, 339, 398, 518, 819, 2200
MspR9I CCNGG 5 cut(s) 102, 203, 340, 2810, 3143
MunI CAATTG 2 cut(s) 1128, 1221
Mva1269I GAATGC 1 cut(s) 2846
MvaI CCWGG 4 cut(s) 102, 203, 2810, 3143
MvnI CGCG 3 cut(s) 59, 186, 1936
NaeI GCCGGC 1 cut(s) 820
NarI GGCGCC 3 cut(s) 77, 126, 3081
NciI CCSGG 1 cut(s) 340
NcoI CCATGG 2 cut(s) 3075, 3217
NdeI CATATG 1 cut(s) 1792
NgoMIV GCCGGC 1 cut(s) 818
NheI GCTAGC 1 cut(s) 2932
NmeAIII GCCGAG 4 cut(s) 214, 794, 2023, 3109
NmuCI GTSAC 4 cut(s) 104, 143, 2195, 3032
NsbI TGCGCA 2 cut(s) 276, 2501
NspI RCATGY 3 cut(s) 175, 995, 1721
NspV TTCGAA 1 cut(s) 3063
PaeI GCATGC 2 cut(s) 175, 1721
PaeR7I CTCGAG 1 cut(s) 413
PagI TCATGA 1 cut(s) 1156
PciSI GCTCTTC 1 cut(s) 1323
PcsI WCGNNNNNNNCGW 2 cut(s) 821, 3033
PctI GAATGC 1 cut(s) 2846
PdiI GCCGGC 1 cut(s) 820
PfeI GAWTC 7 cut(s) 1430, 1448, 1943, 2233, 2365, 2379, 2648
PflMI CCANNNNNTGG 2 cut(s) 1750, 3076
PleI GAGTC 1 cut(s) 79
PluTI GGCGCC 3 cut(s) 80, 129, 3084
PpsI GAGTC 1 cut(s) 79
PshAI GACNNNNGTC 1 cut(s) 1623
Psp124BI GAGCTC 1 cut(s) 3213
Psp1406I AACGTT 1 cut(s) 1339
Psp6I CCWGG 4 cut(s) 100, 201, 2808, 3141
PspEI GGTNACC 2 cut(s) 104, 2195
PspFI CCCAGC 5 cut(s) 389, 781, 1847, 2256, 2599
PspGI CCWGG 4 cut(s) 100, 201, 2808, 3141
PspPI GGNCC 8 cut(s) 821, 873, 1099, 1597, 1865, 2608, 3148, 3285
PstI CTGCAG 1 cut(s) 448
PstNI CAGNNNCTG 1 cut(s) 1979
PsuI RGATCY 4 cut(s) 97, 640, 808, 1534
PvuII CAGCTG 1 cut(s) 1847
RigI GGCCGGCC 1 cut(s) 822
RseI CAYNNNNRTG 3 cut(s) 1206, 1422, 1812
SacI GAGCTC 1 cut(s) 3213
SapI GCTCTTC 1 cut(s) 1323
SaqAI TTAA 6 cut(s) 1323, 2147, 2385, 2472, 2532, 2889
Sau96I GGNCC 8 cut(s) 821, 873, 1099, 1597, 1865, 2608, 3148, 3285
SbfI CCTGCAGG 1 cut(s) 448
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 5 cut(s) 102, 203, 340, 2810, 3143
SdaI CCTGCAGG 1 cut(s) 448
SduI GDGCHC 5 cut(s) 922, 1318, 1789, 3213, 3249
SfaNI GCATC 7 cut(s) 262, 370, 490, 1675, 2200, 2686, 3231
SfcI CTRYAG 5 cut(s) 256, 444, 507, 1108, 2653
SfoI GGCGCC 3 cut(s) 78, 127, 3082
Sfr274I CTCGAG 1 cut(s) 413
SfuI TTCGAA 1 cut(s) 3063
SinI GGWCC 5 cut(s) 873, 1597, 1865, 2608, 3285
SlaI CTCGAG 1 cut(s) 413
SmiMI CAYNNNNRTG 3 cut(s) 1206, 1422, 1812
SmlI CTYRAG 9 cut(s) 81, 296, 332, 413, 771, 1370, 1661, 2156, 2991
SmoI CTYRAG 9 cut(s) 81, 296, 332, 413, 771, 1370, 1661, 2156, 2991
SphI GCATGC 2 cut(s) 175, 1721
Sse8387I CCTGCAGG 1 cut(s) 448
SsiI CCGC 8 cut(s) 57, 166, 169, 564, 1411, 2015, 2539, 2611
SspDI GGCGCC 3 cut(s) 76, 125, 3080
SspI AATATT 4 cut(s) 609, 895, 1087, 1120
SspMI CTAG 7 cut(s) 645, 924, 971, 1266, 2168, 2436, 2933
SstI GAGCTC 1 cut(s) 3213
StyD4I CCNGG 5 cut(s) 100, 201, 338, 2808, 3141
StyI CCWWGG 5 cut(s) 644, 2517, 2627, 3075, 3217
TaaI ACNGT 7 cut(s) 508, 862, 1024, 1112, 2662, 3005, 3112
TaiI ACGT 3 cut(s) 141, 1342, 1973
TatI WGTACW 5 cut(s) 827, 1657, 1810, 1981, 2319
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 7 cut(s) 1430, 1448, 1943, 2233, 2365, 2379, 2648
Tru1I TTAA 6 cut(s) 1323, 2147, 2385, 2472, 2532, 2889
Tru9I TTAA 6 cut(s) 1323, 2147, 2385, 2472, 2532, 2889
TscAI CASTG 5 cut(s) 51, 119, 216, 2034, 2062
TseFI GTSAC 4 cut(s) 104, 143, 2195, 3032
Tsp45I GTSAC 4 cut(s) 104, 143, 2195, 3032
TspGWI ACGGA 3 cut(s) 131, 904, 1952
TspRI CASTG 5 cut(s) 51, 119, 216, 2034, 2062
Van91I CCANNNNNTGG 2 cut(s) 1750, 3076
VneI GTGCAC 2 cut(s) 918, 1785
VpaK11BI GGWCC 5 cut(s) 873, 1597, 1865, 2608, 3285
XapI RAATTY 4 cut(s) 10, 467, 1400, 2939
XbaI TCTAGA 1 cut(s) 2435
XceI RCATGY 3 cut(s) 175, 995, 1721
XcmI CCANNNNNNNNNTGG 2 cut(s) 1303, 1830
XhoI CTCGAG 1 cut(s) 413
XmaJI CCTAGG 1 cut(s) 644
XmiI GTMKAC 2 cut(s) 372, 1478
XspI CTAG 7 cut(s) 645, 924, 971, 1266, 2168, 2436, 2933
ZraI GACGTC 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.