RLG00000008511

Ras-induced vulval development antagonist

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0008597
Physical Location & Seq
Forward (+)
31554671 .. 31556197
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008511

Sequence Viewer

Length: 1527 bp
ATGGCGAGGCCTTCCTCCACCGTTCATGTTCCCAATCGACGATTTCGTTCTGATCACGATAACGGCCGTTACCCGCCGCCGGACTATGACAACTCACCTGACGACCGCCGCCACCGTCGCAGCCCCAGCTATGAAAGGTACGACCGCCACAGAAATCGATCTTATTCTCCTGATTATCCAAGCCCTAGGTCCAACGGAGGGCGAAGATTTGGTCGGGCCGGGAACGACCGGTCTTACTCGCCTCGACGAAGCCCTAGGTCCAACGGAGGGCCGGAGTCTTTACCCAGGAGATTCGGGCGGGCCGGGAACGACAGGGACCGGAATGGGAGGTGGCCGGAGTCGGAGGAGTCCGACGAGGAGCTGAAGGGGCTGAATGTTGAGGAGTATCGGAGGCTGAAGAGGCAGAAGCTGAGGAAGGCGTTGAGGAGTTGTATCTGGAATGTGACACCGAGCCCGCCGAGGAATGAGAACGATGAATTCGAATTGGAGGACAAGGCTGAGGAGATTCCAGAGGTTTTTGGTGAAGAGAACAACGATGCAGGCGAGAAATTGAAGGACAAAGCCAAGAGTGAATCGGAGTCTGAATCCGAATCGGAATCGGAGGATTCAAGGTCGAGAAAGAGGAAGAAGAGTTCGGTTTCTAAGGGTAGGAAGAAGAGTTCGAGATACAGTGATAGTGAATCGGAGAGCGAGAGTGATGTTGAGTCAGATGAGGAAGAGGATCGCAGAAGGAGAAGGAAATCTAGAACAAGGAGTAAGCGAAAGAAGAGCAGAAGAGAAAGGAGAAGAAGGAAGAGTAGGCGGAGCAGCGATTCCGATGAGAGTGAAGCAAGTGATAGTGAGGAGGATTCAGATTCTTCTGATAGGGTAAAGTCGAAGAAGAAGAAGAAGGGAAAGGTGAGCAGTAAGAGTAAGAAGAAGAGGTACTCTGAAACGGAGAGTGAGGATTCTGGTTCAGAGAAGAGTTTGGATTCTGAAGTTGATGCCAAGCCGAAAGCTCTAGTCGAAGTGGAGGAGCCAGAAATGGATGAGAATAGTATTGAGGCCTTGAAATTGAAGGAAATCCTTGAGGCTCAGAGGAACCCTTCATTGGATTTGGATAATGAACCTGTGGTTGGGCCAATGCCCTTGCCTAGAGCCGAAGGGCATATTAGTTATGGTGGAGCGCTTAGGCCTGGTGAAGGTGATGCCATTGCACAGTATGTTCAACAAGGGAAGCGTATTCCGCGAAGAGGAGAAGTTGGTCTTTCTGCAGAGGAGATTCAGAAGTTTGAGGGTCTTGGCTATGTGATGAGTGGTAGTAGGCACCAGAGGATGAATGCCATTCGTATTAGAAAAGAAAACCAGGTTTATAGTGCTGAGGATAAGAGGGCATTGGCCATGTTTAACTATGAGGAGAAAGCAAAGCGTGAGCACAAGGTTATGGCTGATTTGCAGCGGCTGGTTCAGCGCCATATTGGACAGGATGTTGGTCCTACTCACGATCCCTTTTCTTCCAAGGCCTCCGAGGATCCTGATGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SynMuv_product

Protein Analysis

509

Amino Acids

58.55

Weight (kDa)

9.15

Isoelectric Point (pI)

91.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nkap_C PF06047 385 - 483 9.7e-47 NF-kappa-B-activating protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1305
AccII CGCG 1 cut(s) 1228
AclWI GGATC 4 cut(s) 729, 1478, 1505, 1518
AcoI YGGCCR 3 cut(s) 64, 332, 1377
AcsI RAATTY 1 cut(s) 476
AcuI CTGAAG 3 cut(s) 383, 416, 996
AfaI GTAC 2 cut(s) 140, 926
AfeI AGCGCT 1 cut(s) 1167
AfiI CCNNNNNNNGG 7 cut(s) 79, 198, 267, 1090, 1115, 1181, 1232
AgeI ACCGGT 1 cut(s) 228
AgsI TTSAA 5 cut(s) 553, 609, 1051, 1057, 1208
AjnI CCWGG 3 cut(s) 284, 1174, 1344
AjuI GAANNNNNNNTTGG 4 cut(s) 1073, 1098, 1105, 1130
AloI GAACNNNNNNTCC 4 cut(s) 616, 643, 648, 675
AluBI AGCT 4 cut(s) 129, 361, 409, 998
AluI AGCT 4 cut(s) 129, 361, 409, 998
Alw21I GWGCWC 1 cut(s) 1416
AlwI GGATC 4 cut(s) 729, 1478, 1505, 1518
AlwNI CAGNNNCTG 2 cut(s) 409, 1441
Aor51HI AGCGCT 1 cut(s) 1167
ApeKI GCWGC 3 cut(s) 120, 807, 1435
ApoI RAATTY 1 cut(s) 476
ArsI GACNNNNNNTTYG 2 cut(s) 196, 228
AsiGI ACCGGT 1 cut(s) 228
AspA2I CCTAGG 2 cut(s) 185, 254
AspLEI GCGC 2 cut(s) 1168, 1452
AspS9I GGNCC 8 cut(s) 189, 216, 258, 269, 300, 316, 1118, 1472
AsuC2I CCSGG 2 cut(s) 220, 304
AsuHPI GGTGA 5 cut(s) 87, 533, 910, 1190, 1196
AsuII TTCGAA 1 cut(s) 480
AvaII GGWCC 4 cut(s) 189, 258, 316, 1472
AvrII CCTAGG 2 cut(s) 185, 254
BalI TGGCCA 1 cut(s) 1379
BamHI GGATCC 1 cut(s) 1510
BanI GGYRCC 1 cut(s) 1305
BanII GRGCYC 1 cut(s) 455
BarI GAAGNNNNNNTAC 2 cut(s) 908, 940
Bbv12I GWGCWC 1 cut(s) 1416
BbvCI CCTCAGC 3 cut(s) 410, 498, 1359
BbvI GCAGC 3 cut(s) 132, 819, 1447
BceAI ACGGC 2 cut(s) 51, 79
BciT130I CCWGG 3 cut(s) 286, 1176, 1346
BclI TGATCA 1 cut(s) 52
BcnI CCSGG 2 cut(s) 220, 304
BfaI CTAG 5 cut(s) 186, 255, 744, 1001, 1134
BfmI CTRYAG 1 cut(s) 1251
BfoI RGCGCY 2 cut(s) 1169, 1453
BisI GCNGC 6 cut(s) 77, 109, 121, 808, 1436, 1439
BlnI CCTAGG 2 cut(s) 185, 254
BlsI GCNGC 6 cut(s) 78, 110, 122, 809, 1437, 1440
Bme1390I CCNGG 5 cut(s) 220, 286, 304, 1176, 1346
Bme18I GGWCC 4 cut(s) 189, 258, 316, 1472
BmgT120I GGNCC 8 cut(s) 189, 216, 258, 269, 300, 316, 1118, 1472
BmiI GGNNCC 5 cut(s) 317, 1017, 1082, 1307, 1512
BmrFI CCNGG 5 cut(s) 220, 286, 304, 1176, 1346
BmsI GCATC 3 cut(s) 526, 973, 1177
Bpu10I CCTNAGC 4 cut(s) 410, 498, 1169, 1359
Bpu14I TTCGAA 1 cut(s) 480
BpuEI CTTGAG 1 cut(s) 1088
BpuMI CCSGG 2 cut(s) 220, 304
Bsa29I ATCGAT 1 cut(s) 157
BsaJI CCNNGG 6 cut(s) 185, 254, 284, 458, 1497, 1506
BsaWI WCCGGW 2 cut(s) 228, 318
Bsc4I CCNNNNNNNGG 7 cut(s) 79, 198, 267, 1090, 1115, 1181, 1232
Bse118I RCCGGY 1 cut(s) 228
Bse3DI GCAATG 1 cut(s) 1191
BseBI CCWGG 3 cut(s) 286, 1176, 1346
BseCI ATCGAT 1 cut(s) 157
BseDI CCNNGG 6 cut(s) 185, 254, 284, 458, 1497, 1506
BseGI GGATG 3 cut(s) 1033, 1320, 1471
BseLI CCNNNNNNNGG 7 cut(s) 79, 198, 267, 1090, 1115, 1181, 1232
BseMI GCAATG 1 cut(s) 1191
BseMII CTCAG 4 cut(s) 401, 489, 1088, 1350
BseX3I CGGCCG 1 cut(s) 64
BseXI GCAGC 3 cut(s) 132, 819, 1447
BseYI CCCAGC 1 cut(s) 125
Bsh1236I CGCG 1 cut(s) 1228
Bsh1285I CGRYCG 4 cut(s) 67, 106, 145, 229
BshNI GGYRCC 1 cut(s) 1305
BshTI ACCGGT 1 cut(s) 228
BshVI ATCGAT 1 cut(s) 157
BsiEI CGRYCG 4 cut(s) 67, 106, 145, 229
BsiHKAI GWGCWC 1 cut(s) 1416
BsiSI CCGG 7 cut(s) 80, 219, 229, 272, 303, 319, 335
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 7 cut(s) 79, 198, 267, 1090, 1115, 1181, 1232
BsmFI GGGAC 1 cut(s) 329
BsmI GAATGC 1 cut(s) 1324
Bsp119I TTCGAA 1 cut(s) 480
Bsp1286I GDGCHC 2 cut(s) 455, 1416
Bsp143I GATC 5 cut(s) 52, 158, 721, 1483, 1510
BspCNI CTCAG 4 cut(s) 402, 490, 1087, 1351
BspDI ATCGAT 1 cut(s) 157
BspFNI CGCG 1 cut(s) 1228
BspLI GGNNCC 5 cut(s) 317, 1017, 1082, 1307, 1512
BspMAI CTGCAG 1 cut(s) 1255
BspPI GGATC 4 cut(s) 729, 1478, 1505, 1518
BspQI GCTCTTC 1 cut(s) 761
BspT104I TTCGAA 1 cut(s) 480
BspT107I GGYRCC 1 cut(s) 1305
BsrDI GCAATG 1 cut(s) 1191
BsrFI RCCGGY 1 cut(s) 228
BssAI RCCGGY 1 cut(s) 228
BssECI CCNNGG 6 cut(s) 185, 254, 284, 458, 1497, 1506
BssMI GATC 5 cut(s) 52, 158, 721, 1483, 1510
BssT1I CCWWGG 3 cut(s) 185, 254, 1497
Bst2UI CCWGG 3 cut(s) 286, 1176, 1346
Bst4CI ACNGT 4 cut(s) 22, 116, 671, 1200
BstBI TTCGAA 1 cut(s) 480
BstC8I GCNNGC 3 cut(s) 300, 455, 541
BstDEI CTNAG 6 cut(s) 410, 498, 642, 1074, 1169, 1359
BstENI CCTNNNNNAGG 1 cut(s) 1179
BstF5I GGATG 3 cut(s) 1033, 1320, 1471
BstFNI CGCG 1 cut(s) 1228
BstH2I RGCGCY 2 cut(s) 1169, 1453
BstHHI GCGC 2 cut(s) 1168, 1452
BstKTI GATC 5 cut(s) 55, 161, 724, 1486, 1513
BstMBI GATC 5 cut(s) 52, 158, 721, 1483, 1510
BstMCI CGRYCG 4 cut(s) 67, 106, 145, 229
BstMWI GCNNNNNNNGC 6 cut(s) 117, 126, 367, 400, 1225, 1447
BstNI CCWGG 3 cut(s) 286, 1176, 1346
BstSCI CCNGG 5 cut(s) 218, 284, 302, 1174, 1344
BstSFI CTRYAG 1 cut(s) 1251
BstUI CGCG 1 cut(s) 1228
BstV1I GCAGC 3 cut(s) 132, 819, 1447
BstX2I RGATCY 1 cut(s) 1510
BstYI RGATCY 1 cut(s) 1510
BstZI CGGCCG 1 cut(s) 64
Bsu15I ATCGAT 1 cut(s) 157
BsuTUI ATCGAT 1 cut(s) 157
BtsCI GGATG 3 cut(s) 1033, 1320, 1471
BtsIMutI CAGTG 1 cut(s) 676
Cac8I GCNNGC 3 cut(s) 300, 455, 541
CaiI CAGNNNCTG 2 cut(s) 409, 1441
CfoI GCGC 2 cut(s) 1168, 1452
Cfr10I RCCGGY 1 cut(s) 228
Cfr13I GGNCC 8 cut(s) 189, 216, 258, 269, 300, 316, 1118, 1472
ClaI ATCGAT 1 cut(s) 157
CsiI ACCWGGT 1 cut(s) 1344
Csp6I GTAC 2 cut(s) 139, 925
CspAI ACCGGT 1 cut(s) 228
CviAII CATG 2 cut(s) 26, 1381
CviQI GTAC 2 cut(s) 139, 925
DdeI CTNAG 6 cut(s) 410, 498, 642, 1074, 1169, 1359
DpnI GATC 5 cut(s) 54, 160, 723, 1485, 1512
DpnII GATC 5 cut(s) 52, 158, 721, 1483, 1510
EaeI YGGCCR 3 cut(s) 64, 332, 1377
EagI CGGCCG 1 cut(s) 64
EciI GGCGGA 1 cut(s) 817
EclXI CGGCCG 1 cut(s) 64
Eco130I CCWWGG 3 cut(s) 185, 254, 1497
Eco147I AGGCCT 4 cut(s) 10, 1046, 1174, 1502
Eco24I GRGCYC 1 cut(s) 455
Eco47I GGWCC 4 cut(s) 189, 258, 316, 1472
Eco47III AGCGCT 1 cut(s) 1167
Eco52I CGGCCG 1 cut(s) 64
Eco57I CTGAAG 3 cut(s) 383, 416, 996
EcoNI CCTNNNNNAGG 1 cut(s) 1179
EcoRI GAATTC 1 cut(s) 476
EcoRII CCWGG 3 cut(s) 284, 1174, 1344
EcoT14I CCWWGG 3 cut(s) 185, 254, 1497
EcoT38I GRGCYC 1 cut(s) 455
ErhI CCWWGG 3 cut(s) 185, 254, 1497
FaeI CATG 2 cut(s) 29, 1384
FalI AAGNNNNNCTT 2 cut(s) 1230, 1262
FaqI GGGAC 1 cut(s) 329
FatI CATG 2 cut(s) 25, 1380
FauI CCCGC 3 cut(s) 81, 291, 462
FbaI TGATCA 1 cut(s) 52
Fnu4HI GCNGC 6 cut(s) 77, 109, 121, 808, 1436, 1439
FokI GGATG 3 cut(s) 1040, 1327, 1478
FriOI GRGCYC 1 cut(s) 455
Fsp4HI GCNGC 6 cut(s) 77, 109, 121, 808, 1436, 1439
FspBI CTAG 5 cut(s) 186, 255, 744, 1001, 1134
GlaI GCGC 2 cut(s) 1167, 1451
GluI GCNGC 6 cut(s) 77, 109, 121, 808, 1436, 1439
GsaI CCCAGC 1 cut(s) 129
HaeII RGCGCY 2 cut(s) 1169, 1453
HapII CCGG 7 cut(s) 80, 219, 229, 272, 303, 319, 335
HhaI GCGC 2 cut(s) 1168, 1452
Hin1II CATG 2 cut(s) 29, 1384
Hin6I GCGC 2 cut(s) 1166, 1450
HinP1I GCGC 2 cut(s) 1166, 1450
HpaII CCGG 7 cut(s) 80, 219, 229, 272, 303, 319, 335
HphI GGTGA 5 cut(s) 87, 533, 910, 1190, 1196
Hpy188III TCNNGA 9 cut(s) 56, 170, 436, 509, 615, 663, 744, 1481, 1514
Hpy99I CGWCG 4 cut(s) 42, 120, 249, 356
HpyCH4III ACNGT 4 cut(s) 22, 116, 671, 1200
HpyCH4V TGCA 4 cut(s) 539, 1196, 1253, 1435
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 126, 367, 400, 1225, 1447
HpyF3I CTNAG 6 cut(s) 410, 498, 642, 1074, 1169, 1359
Hsp92II CATG 2 cut(s) 29, 1384
HspAI GCGC 2 cut(s) 1166, 1450
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 5 cut(s) 52, 158, 721, 1483, 1510
LguI GCTCTTC 1 cut(s) 761
LmnI GCTCC 4 cut(s) 358, 804, 1015, 1163
Lsp1109I GCAGC 3 cut(s) 132, 819, 1447
LweI GCATC 3 cut(s) 526, 973, 1177
MabI ACCWGGT 1 cut(s) 1344
MaeI CTAG 5 cut(s) 186, 255, 744, 1001, 1134
MaeIII GTNAC 2 cut(s) 68, 442
MalI GATC 5 cut(s) 54, 160, 723, 1485, 1512
MboI GATC 5 cut(s) 52, 158, 721, 1483, 1510
MflI RGATCY 1 cut(s) 1510
MhlI GDGCHC 2 cut(s) 455, 1416
MlsI TGGCCA 1 cut(s) 1379
MluCI AATT 4 cut(s) 476, 482, 548, 1052
MluNI TGGCCA 1 cut(s) 1379
MlyI GAGTC 5 cut(s) 284, 347, 356, 587, 713
MmeI TCCRAC 4 cut(s) 216, 285, 321, 375
Mox20I TGGCCA 1 cut(s) 1379
MscI TGGCCA 1 cut(s) 1379
MseI TTAA 1 cut(s) 1386
Msp20I TGGCCA 1 cut(s) 1379
MspA1I CMGCKG 1 cut(s) 1438
MspI CCGG 7 cut(s) 80, 219, 229, 272, 303, 319, 335
MspR9I CCNGG 5 cut(s) 220, 286, 304, 1176, 1346
Mva1269I GAATGC 1 cut(s) 1324
MvaI CCWGG 3 cut(s) 286, 1176, 1346
MvnI CGCG 1 cut(s) 1228
MwoI GCNNNNNNNGC 6 cut(s) 117, 126, 367, 400, 1225, 1447
NciI CCSGG 2 cut(s) 220, 304
NdeII GATC 5 cut(s) 52, 158, 721, 1483, 1510
NlaIII CATG 2 cut(s) 29, 1384
NlaIV GGNNCC 5 cut(s) 317, 1017, 1082, 1307, 1512
NmeAIII GCCGAG 1 cut(s) 483
NmuCI GTSAC 1 cut(s) 442
NspV TTCGAA 1 cut(s) 480
PceI AGGCCT 4 cut(s) 10, 1046, 1174, 1502
PciSI GCTCTTC 1 cut(s) 761
PcsI WCGNNNNNNNCGW 3 cut(s) 43, 477, 540
PctI GAATGC 1 cut(s) 1324
PinAI ACCGGT 1 cut(s) 228
PkrI GCNGC 6 cut(s) 78, 110, 122, 809, 1437, 1440
PleI GAGTC 5 cut(s) 283, 346, 355, 586, 712
PpsI GAGTC 5 cut(s) 283, 346, 355, 586, 712
Psp6I CCWGG 3 cut(s) 284, 1174, 1344
PspFI CCCAGC 1 cut(s) 125
PspGI CCWGG 3 cut(s) 284, 1174, 1344
PspN4I GGNNCC 5 cut(s) 317, 1017, 1082, 1307, 1512
PspPI GGNCC 8 cut(s) 189, 216, 258, 269, 300, 316, 1118, 1472
PstI CTGCAG 1 cut(s) 1255
PstNI CAGNNNCTG 2 cut(s) 409, 1441
PsuI RGATCY 1 cut(s) 1510
RsaI GTAC 2 cut(s) 140, 926
RsaNI GTAC 2 cut(s) 139, 925
SapI GCTCTTC 1 cut(s) 761
SaqAI TTAA 1 cut(s) 1386
SatI GCNGC 6 cut(s) 77, 109, 121, 808, 1436, 1439
Sau3AI GATC 5 cut(s) 52, 158, 721, 1483, 1510
Sau96I GGNCC 8 cut(s) 189, 216, 258, 269, 300, 316, 1118, 1472
SchI GAGTC 5 cut(s) 284, 347, 356, 587, 713
ScrFI CCNGG 5 cut(s) 220, 286, 304, 1176, 1346
SduI GDGCHC 2 cut(s) 455, 1416
SexAI ACCWGGT 1 cut(s) 1344
SfaNI GCATC 3 cut(s) 526, 973, 1177
SfcI CTRYAG 1 cut(s) 1251
SfuI TTCGAA 1 cut(s) 480
SinI GGWCC 4 cut(s) 189, 258, 316, 1472
SmlI CTYRAG 1 cut(s) 1067
SmoI CTYRAG 1 cut(s) 1067
Sse9I AATT 4 cut(s) 476, 482, 548, 1052
SseBI AGGCCT 4 cut(s) 10, 1046, 1174, 1502
SspMI CTAG 5 cut(s) 186, 255, 744, 1001, 1134
StuI AGGCCT 4 cut(s) 10, 1046, 1174, 1502
StyD4I CCNGG 5 cut(s) 218, 284, 302, 1174, 1344
StyI CCWWGG 3 cut(s) 185, 254, 1497
TaaI ACNGT 4 cut(s) 22, 116, 671, 1200
TaqI TCGA 8 cut(s) 37, 157, 244, 480, 614, 662, 875, 1005
TasI AATT 4 cut(s) 476, 482, 548, 1052
TauI GCSGC 3 cut(s) 79, 111, 1441
Tru1I TTAA 1 cut(s) 1386
Tru9I TTAA 1 cut(s) 1386
TscAI CASTG 1 cut(s) 676
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 3 cut(s) 120, 807, 1435
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 6 cut(s) 14, 147, 489, 1077, 1119, 1331
TspGWI ACGGA 3 cut(s) 210, 279, 950
TspRI CASTG 1 cut(s) 676
VpaK11BI GGWCC 4 cut(s) 189, 258, 316, 1472
XagI CCTNNNNNAGG 1 cut(s) 1179
XapI RAATTY 1 cut(s) 476
XbaI TCTAGA 1 cut(s) 743
XmaJI CCTAGG 2 cut(s) 185, 254
XspI CTAG 5 cut(s) 186, 255, 744, 1001, 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.