Rh4DG153300

Ras-induced vulval development antagonist

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008597
Physical Location & Seq
Reverse (-)
30512904 .. 30513407
504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG153300.1

Sequence Viewer

Length: 504 bp
ATGGATGAGAATAGTATTGAGGCCTTGAAATTGAAGGAAATCCTTGAGGCTCAGAGGAACCCTTCTTTGGATTTGGATAATGAACCTGTGGTTGGGCCAATGCCCTTGCCTAGAGCCGAAGGGCATATTAGTTATGGTGGAGCGCTTAGGCCTGGTGAAGGTGATGCCATTGCACAGTATGTTCAACAAGGGAAGCGTATTCCACGAAGAGGAGAAGTTGGTCTTTCTGCAGAGGAGATTCAGAAGTTTGAGGGTCTTGGCTATGTGATGAGTGGTAGTAGGCACCAGAGGATGAATGCCATTCGTATTAGAAAGGAAAACCAGGTTTATAGTGCTGAGGATAAGAGGGCATTGGCCATGTTTAACTATGAGGAGAAAGCAAAGCGTGAGCACAAGGTTATGGCTGATTTGCAGCGGCTGGTTCAGCGCCATATTGGACAGGATGTTGGTCCTACTCACGATCCCTTTTCTTCCAAGGCCTCCGAGGATCCTGATGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SynMuv_product

Protein Analysis

167

Amino Acids

18.76

Weight (kDa)

5.6

Isoelectric Point (pI)

50.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nkap_C PF06047 44 - 142 8.9e-48 NF-kappa-B-activating protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 282
AciI CCGC 1 cut(s) 415
AclWI GGATC 3 cut(s) 455, 482, 495
AcoI YGGCCR 1 cut(s) 354
AfeI AGCGCT 1 cut(s) 144
AfiI CCNNNNNNNGG 4 cut(s) 67, 92, 158, 209
AgsI TTSAA 3 cut(s) 28, 34, 185
AjnI CCWGG 2 cut(s) 151, 321
AjuI GAANNNNNNNTTGG 4 cut(s) 50, 75, 82, 107
Alw21I GWGCWC 1 cut(s) 393
AlwI GGATC 3 cut(s) 455, 482, 495
AlwNI CAGNNNCTG 1 cut(s) 418
Aor51HI AGCGCT 1 cut(s) 144
AoxI GGCC 5 cut(s) 21, 95, 149, 354, 477
ApeKI GCWGC 1 cut(s) 412
AspLEI GCGC 2 cut(s) 145, 429
AspS9I GGNCC 2 cut(s) 95, 449
AsuHPI GGTGA 2 cut(s) 167, 173
AvaII GGWCC 1 cut(s) 449
BalI TGGCCA 1 cut(s) 356
BamHI GGATCC 1 cut(s) 487
BanI GGYRCC 1 cut(s) 282
Bbv12I GWGCWC 1 cut(s) 393
BbvCI CCTCAGC 1 cut(s) 336
BbvI GCAGC 1 cut(s) 424
BciT130I CCWGG 2 cut(s) 153, 323
BfaI CTAG 1 cut(s) 111
BfmI CTRYAG 1 cut(s) 228
BfoI RGCGCY 2 cut(s) 146, 430
BisI GCNGC 2 cut(s) 413, 416
BlsI GCNGC 2 cut(s) 414, 417
Bme1390I CCNGG 2 cut(s) 153, 323
Bme18I GGWCC 1 cut(s) 449
BmgT120I GGNCC 2 cut(s) 95, 449
BmiI GGNNCC 3 cut(s) 59, 284, 489
BmrFI CCNGG 2 cut(s) 153, 323
BmsI GCATC 1 cut(s) 154
Bpu10I CCTNAGC 2 cut(s) 146, 336
BpuEI CTTGAG 1 cut(s) 65
BsaJI CCNNGG 2 cut(s) 474, 483
Bsc4I CCNNNNNNNGG 4 cut(s) 67, 92, 158, 209
Bse3DI GCAATG 1 cut(s) 168
BseBI CCWGG 2 cut(s) 153, 323
BseDI CCNNGG 2 cut(s) 474, 483
BseGI GGATG 3 cut(s) 10, 297, 448
BseLI CCNNNNNNNGG 4 cut(s) 67, 92, 158, 209
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 2 cut(s) 65, 327
BseRI GAGGAG 3 cut(s) 225, 248, 386
BseXI GCAGC 1 cut(s) 424
BshFI GGCC 5 cut(s) 23, 97, 151, 356, 479
BshNI GGYRCC 1 cut(s) 282
BsiHKAI GWGCWC 1 cut(s) 393
BslI CCNNNNNNNGG 4 cut(s) 67, 92, 158, 209
BsmI GAATGC 1 cut(s) 301
BsnI GGCC 5 cut(s) 23, 97, 151, 356, 479
Bsp1286I GDGCHC 1 cut(s) 393
Bsp143I GATC 2 cut(s) 460, 487
BspACI CCGC 1 cut(s) 415
BspANI GGCC 5 cut(s) 23, 97, 151, 356, 479
BspCNI CTCAG 2 cut(s) 64, 328
BspLI GGNNCC 3 cut(s) 59, 284, 489
BspMAI CTGCAG 1 cut(s) 232
BspPI GGATC 3 cut(s) 455, 482, 495
BspT107I GGYRCC 1 cut(s) 282
BsrDI GCAATG 1 cut(s) 168
BssECI CCNNGG 2 cut(s) 474, 483
BssMI GATC 2 cut(s) 460, 487
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 2 cut(s) 153, 323
Bst4CI ACNGT 1 cut(s) 177
Bst6I CTCTTC 1 cut(s) 202
BstDEI CTNAG 3 cut(s) 51, 146, 336
BstENI CCTNNNNNAGG 1 cut(s) 156
BstF5I GGATG 3 cut(s) 10, 297, 448
BstH2I RGCGCY 2 cut(s) 146, 430
BstHHI GCGC 2 cut(s) 145, 429
BstKTI GATC 2 cut(s) 463, 490
BstMBI GATC 2 cut(s) 460, 487
BstMWI GCNNNNNNNGC 1 cut(s) 424
BstNI CCWGG 2 cut(s) 153, 323
BstSCI CCNGG 2 cut(s) 151, 321
BstSFI CTRYAG 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 424
BstX2I RGATCY 1 cut(s) 487
BstYI RGATCY 1 cut(s) 487
BsuRI GGCC 5 cut(s) 23, 97, 151, 356, 479
BtsCI GGATG 3 cut(s) 10, 297, 448
CaiI CAGNNNCTG 1 cut(s) 418
CfoI GCGC 2 cut(s) 145, 429
Cfr13I GGNCC 2 cut(s) 95, 449
CsiI ACCWGGT 1 cut(s) 321
CviAII CATG 1 cut(s) 358
DdeI CTNAG 3 cut(s) 51, 146, 336
DpnI GATC 2 cut(s) 462, 489
DpnII GATC 2 cut(s) 460, 487
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 1 cut(s) 202
EarI CTCTTC 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 474
Eco147I AGGCCT 3 cut(s) 23, 151, 479
Eco47I GGWCC 1 cut(s) 449
Eco47III AGCGCT 1 cut(s) 144
EcoNI CCTNNNNNAGG 1 cut(s) 156
EcoRII CCWGG 2 cut(s) 151, 321
EcoT14I CCWWGG 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 1 cut(s) 361
FaiI YATR 9 cut(s) 126, 135, 180, 264, 330, 359, 369, 401, 432
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FatI CATG 1 cut(s) 357
Fnu4HI GCNGC 2 cut(s) 413, 416
FokI GGATG 3 cut(s) 17, 304, 455
Fsp4HI GCNGC 2 cut(s) 413, 416
FspBI CTAG 1 cut(s) 111
GlaI GCGC 2 cut(s) 144, 428
GluI GCNGC 2 cut(s) 413, 416
HaeII RGCGCY 2 cut(s) 146, 430
HaeIII GGCC 5 cut(s) 23, 97, 151, 356, 479
HhaI GCGC 2 cut(s) 145, 429
Hin1II CATG 1 cut(s) 361
Hin6I GCGC 2 cut(s) 143, 427
HinP1I GCGC 2 cut(s) 143, 427
HinfI GANTC 1 cut(s) 238
HphI GGTGA 2 cut(s) 167, 173
Hpy188I TCNGA 3 cut(s) 54, 243, 484
Hpy188III TCNNGA 2 cut(s) 458, 491
HpyAV CCTTC 4 cut(s) 28, 72, 113, 152
HpyCH4III ACNGT 1 cut(s) 177
HpyCH4V TGCA 3 cut(s) 173, 230, 412
HpyF10VI GCNNNNNNNGC 1 cut(s) 424
HpyF3I CTNAG 3 cut(s) 51, 146, 336
Hsp92II CATG 1 cut(s) 361
HspAI GCGC 2 cut(s) 143, 427
Kzo9I GATC 2 cut(s) 460, 487
LmnI GCTCC 1 cut(s) 140
LpnPI CCDG 8 cut(s) 99, 138, 165, 299, 308, 335, 404, 425
Lsp1109I GCAGC 1 cut(s) 424
LweI GCATC 1 cut(s) 154
MabI ACCWGGT 1 cut(s) 321
MaeI CTAG 1 cut(s) 111
MalI GATC 2 cut(s) 462, 489
MboI GATC 2 cut(s) 460, 487
MboII GAAGA 2 cut(s) 219, 462
MflI RGATCY 1 cut(s) 487
MhlI GDGCHC 1 cut(s) 393
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 1 cut(s) 29
MluNI TGGCCA 1 cut(s) 356
Mox20I TGGCCA 1 cut(s) 356
MscI TGGCCA 1 cut(s) 356
MseI TTAA 1 cut(s) 363
Msp20I TGGCCA 1 cut(s) 356
MspA1I CMGCKG 1 cut(s) 415
MspR9I CCNGG 2 cut(s) 153, 323
Mva1269I GAATGC 1 cut(s) 301
MvaI CCWGG 2 cut(s) 153, 323
MwoI GCNNNNNNNGC 1 cut(s) 424
NdeII GATC 2 cut(s) 460, 487
NlaIII CATG 1 cut(s) 361
NlaIV GGNNCC 3 cut(s) 59, 284, 489
PceI AGGCCT 3 cut(s) 23, 151, 479
PctI GAATGC 1 cut(s) 301
PfeI GAWTC 1 cut(s) 238
PkrI GCNGC 2 cut(s) 414, 417
Psp6I CCWGG 2 cut(s) 151, 321
PspGI CCWGG 2 cut(s) 151, 321
PspN4I GGNNCC 3 cut(s) 59, 284, 489
PspPI GGNCC 2 cut(s) 95, 449
PstI CTGCAG 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 418
PsuI RGATCY 1 cut(s) 487
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 2 cut(s) 413, 416
Sau3AI GATC 2 cut(s) 460, 487
Sau96I GGNCC 2 cut(s) 95, 449
ScrFI CCNGG 2 cut(s) 153, 323
SduI GDGCHC 1 cut(s) 393
SetI ASST 4 cut(s) 88, 163, 327, 399
SexAI ACCWGGT 1 cut(s) 321
SfaNI GCATC 1 cut(s) 154
SfcI CTRYAG 1 cut(s) 228
SinI GGWCC 1 cut(s) 449
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 1 cut(s) 29
SseBI AGGCCT 3 cut(s) 23, 151, 479
SsiI CCGC 1 cut(s) 415
SspMI CTAG 1 cut(s) 111
StuI AGGCCT 3 cut(s) 23, 151, 479
StyD4I CCNGG 2 cut(s) 151, 321
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 1 cut(s) 177
TasI AATT 1 cut(s) 29
TauI GCSGC 1 cut(s) 418
TfiI GAWTC 1 cut(s) 238
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TseI GCWGC 1 cut(s) 412
TspDTI ATGAA 2 cut(s) 96, 308
VpaK11BI GGWCC 1 cut(s) 449
XagI CCTNNNNNAGG 1 cut(s) 156
XspI CTAG 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.