Rh4AG158600

Ras-induced vulval development antagonist

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0008597
Physical Location & Seq
Reverse (-)
40289760 .. 40296134
6375 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG158600.1

Sequence Viewer

Length: 1503 bp
ATGGCGAGGCCTTCTTCCACCGTTCATGCCCCCAATCGACGATTCCGTTCTGATCACGATAACGGCCGTTACTCCCCGCCGGACTATGACAACTCACCCGACGACCGCCGCCACCGTCGCAGCCCCAGCTATGAAAGGTACGACCGCCACCGAAATAGCTCTTACTCTCCTGATTACCCTAGCCCTAGGTCCAACGGAGGCCGAAGATTCGGTCGGGCCGGGAACGACCGGTCTTACTCGCCTCGACGAAGCCCTAGGTCCAACGGAGGGCCGGAGTCTTTACCCAGGAGATTCGGGCGCGCCGGGAACGATAGGGACCGGAATGGAAGGTGGCCGGAGTCGGAGGAGTCCGACGAGGAGCTGAAGGGGCTGAATCTTGAGGAGTACCGAAGGCTGAAGAGGCAGAAGCTGAGGAAGGCGTTGAGGAGTTGTATCTGGAATGTGACGCCGAGCCCGCCGAGGAATGAGAACGATGAATTCGAATTGGAGGACAAGGCTGAGGAGATTCCAGAGGTTCTTGGTGAAGAGAACAACGATTCAGGCGAGAAATTGAAGGACAAAGCCAGGAGTGAATCGGAGTCGGAATCGGAGGATTCGCGGTCGAGAAAGAGGAAGAAGAGTTCGGTTTCTAAGCGTAGGAAGAAGAGTTCGAGATACAGTGATAGCGAATCGGAGAGCGAGAGTGATGATGAGGAAGAGGATCGCAGAAGGAGAAAGAAATCTAGAACTAGGAGTAAGCGAAAGAAGAGCAGAAGAGAAAGGAGAAGAAGGAAGAGTAGGCGGAGCAGCGATTCTGATGAGAGTGAAGCAAGTGATAGTGAGGAGGGTTCAGATTCTTCTGATAGGGTAAAGTCGAAGAAGAAGAAGGGAAAGGTGAGCAGTAAGAGTAAGAAGAAGAAGTACTCTGAAACGGAGAGTGAGGATTCTGGTTCAGAGAAGAGTTTGGATTCTGAAGTTGATGCCAAGCCTAAAGCTCTAGTCGAAGTGGAGGAGCCAGAAATGGATGAGAATAGTATTGAGGCCTTGAAATTGAAGGAAATCCTTGAGGCTCAGAGGAACCCTTCTTTGGATTTGGATAATGAACCTGTGGTTGGGCCAATGCCCTTGCCTAGAGCCGAAGGGCATATTAGTTATGGTGGAGCGCTTAGGCCTGGTGAAGGTGATGCCATTGCACAGTATGTTCAACAAGGGAAGCGTATTCCACGAAGAGGAGAAGTTGGTCTTTCTGCAGAGGAGATTCAGAAGTTTGAGGGTCTTGGCTATGTGATGAGTGGTAGTAGGCACCAGAGGATGAATGCCATTCGTATTAGAAAGGAAAACCAGGTTTATAGTGCTGAGGATAAGAGGGCATTGGCCATGTTTAACTATGAGGAGAAAGCAAAGCGTGAGCACAAGGTTATGGCTGATTTGCAGCGGCTGGTTCAGCGCCATATTGGACAGGATGTTGGTCCTACTCACGATCCCTTTTCTTCCAAGGCCTCTGAGGATCCTGATGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SynMuv_product

Protein Analysis

500

Amino Acids

57.58

Weight (kDa)

9.32

Isoelectric Point (pI)

91.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nkap_C PF06047 377 - 475 9.4e-47 NF-kappa-B-activating protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1281
AccII CGCG 2 cut(s) 300, 598
AciI CCGC 8 cut(s) 77, 106, 109, 145, 455, 598, 781, 1414
AclWI GGATC 4 cut(s) 708, 1454, 1481, 1494
AcoI YGGCCR 3 cut(s) 64, 332, 1353
AcsI RAATTY 1 cut(s) 476
AcuI CTGAAG 3 cut(s) 383, 416, 972
AcyI GRCGYC 1 cut(s) 446
AfaI GTAC 3 cut(s) 140, 386, 902
AfeI AGCGCT 1 cut(s) 1143
AfiI CCNNNNNNNGG 5 cut(s) 267, 1066, 1091, 1157, 1208
AgeI ACCGGT 1 cut(s) 228
AgsI TTSAA 4 cut(s) 553, 1027, 1033, 1184
AjnI CCWGG 4 cut(s) 284, 563, 1150, 1320
AjuI GAANNNNNNNTTGG 6 cut(s) 26, 58, 1049, 1074, 1081, 1106
AloI GAACNNNNNNTCC 4 cut(s) 604, 631, 636, 663
AluBI AGCT 5 cut(s) 129, 159, 361, 409, 974
AluI AGCT 5 cut(s) 129, 159, 361, 409, 974
Alw21I GWGCWC 1 cut(s) 1392
AlwI GGATC 4 cut(s) 708, 1454, 1481, 1494
AlwNI CAGNNNCTG 2 cut(s) 409, 1417
Aor51HI AGCGCT 1 cut(s) 1143
ApeKI GCWGC 3 cut(s) 120, 786, 1411
ApoI RAATTY 1 cut(s) 476
ArsI GACNNNNNNTTYG 2 cut(s) 196, 228
AscI GGCGCGCC 1 cut(s) 298
AsiGI ACCGGT 1 cut(s) 228
AspA2I CCTAGG 2 cut(s) 185, 254
AspLEI GCGC 4 cut(s) 300, 302, 1144, 1428
AspS9I GGNCC 7 cut(s) 189, 216, 258, 269, 316, 1094, 1448
AsuC2I CCSGG 2 cut(s) 220, 304
AsuHPI GGTGA 5 cut(s) 87, 533, 886, 1166, 1172
AsuII TTCGAA 1 cut(s) 480
AvaII GGWCC 4 cut(s) 189, 258, 316, 1448
AvrII CCTAGG 2 cut(s) 185, 254
BalI TGGCCA 1 cut(s) 1355
BamHI GGATCC 1 cut(s) 1486
BanI GGYRCC 1 cut(s) 1281
BanII GRGCYC 1 cut(s) 455
BarI GAAGNNNNNNTAC 2 cut(s) 884, 916
Bbv12I GWGCWC 1 cut(s) 1392
BbvCI CCTCAGC 3 cut(s) 410, 498, 1335
BbvI GCAGC 3 cut(s) 132, 798, 1423
BceAI ACGGC 2 cut(s) 51, 79
BciT130I CCWGG 4 cut(s) 286, 565, 1152, 1322
BclI TGATCA 1 cut(s) 52
BcnI CCSGG 2 cut(s) 220, 304
BfaI CTAG 7 cut(s) 180, 186, 255, 723, 729, 977, 1110
BfmI CTRYAG 1 cut(s) 1227
BfoI RGCGCY 2 cut(s) 1145, 1429
BisI GCNGC 5 cut(s) 109, 121, 787, 1412, 1415
BlnI CCTAGG 2 cut(s) 185, 254
BlsI GCNGC 5 cut(s) 110, 122, 788, 1413, 1416
BmcAI AGTACT 1 cut(s) 902
Bme1390I CCNGG 6 cut(s) 220, 286, 304, 565, 1152, 1322
Bme18I GGWCC 4 cut(s) 189, 258, 316, 1448
BmgT120I GGNCC 7 cut(s) 189, 216, 258, 269, 316, 1094, 1448
BmiI GGNNCC 5 cut(s) 317, 993, 1058, 1283, 1488
BmrFI CCNGG 6 cut(s) 220, 286, 304, 565, 1152, 1322
BmsI GCATC 2 cut(s) 949, 1153
Bpu10I CCTNAGC 4 cut(s) 410, 498, 1145, 1335
Bpu14I TTCGAA 1 cut(s) 480
BpuEI CTTGAG 2 cut(s) 398, 1064
BpuMI CCSGG 2 cut(s) 220, 304
BsaHI GRCGYC 1 cut(s) 446
BsaJI CCNNGG 5 cut(s) 185, 254, 284, 458, 1473
BsaWI WCCGGW 2 cut(s) 228, 318
Bsc4I CCNNNNNNNGG 5 cut(s) 267, 1066, 1091, 1157, 1208
Bse118I RCCGGY 1 cut(s) 228
Bse3DI GCAATG 1 cut(s) 1167
BseBI CCWGG 4 cut(s) 286, 565, 1152, 1322
BseDI CCNNGG 5 cut(s) 185, 254, 284, 458, 1473
BseGI GGATG 3 cut(s) 1009, 1296, 1447
BseLI CCNNNNNNNGG 5 cut(s) 267, 1066, 1091, 1157, 1208
BseMI GCAATG 1 cut(s) 1167
BseMII CTCAG 5 cut(s) 401, 489, 1064, 1326, 1473
BsePI GCGCGC 1 cut(s) 298
BseX3I CGGCCG 1 cut(s) 64
BseXI GCAGC 3 cut(s) 132, 798, 1423
BseYI CCCAGC 1 cut(s) 125
Bsh1236I CGCG 2 cut(s) 300, 598
Bsh1285I CGRYCG 6 cut(s) 67, 106, 145, 214, 229, 602
BshNI GGYRCC 1 cut(s) 1281
BshTI ACCGGT 1 cut(s) 228
BsiEI CGRYCG 6 cut(s) 67, 106, 145, 214, 229, 602
BsiHKAI GWGCWC 1 cut(s) 1392
BsiSI CCGG 7 cut(s) 80, 219, 229, 272, 303, 319, 335
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 5 cut(s) 267, 1066, 1091, 1157, 1208
BsmFI GGGAC 1 cut(s) 329
BsmI GAATGC 1 cut(s) 1300
Bsp119I TTCGAA 1 cut(s) 480
Bsp1286I GDGCHC 2 cut(s) 455, 1392
Bsp143I GATC 4 cut(s) 52, 700, 1459, 1486
BspACI CCGC 8 cut(s) 77, 106, 109, 145, 455, 598, 781, 1414
BspCNI CTCAG 5 cut(s) 402, 490, 1063, 1327, 1474
BspFNI CGCG 2 cut(s) 300, 598
BspLI GGNNCC 5 cut(s) 317, 993, 1058, 1283, 1488
BspMAI CTGCAG 1 cut(s) 1231
BspPI GGATC 4 cut(s) 708, 1454, 1481, 1494
BspQI GCTCTTC 1 cut(s) 740
BspT104I TTCGAA 1 cut(s) 480
BspT107I GGYRCC 1 cut(s) 1281
BsrDI GCAATG 1 cut(s) 1167
BsrFI RCCGGY 1 cut(s) 228
BssAI RCCGGY 1 cut(s) 228
BssECI CCNNGG 5 cut(s) 185, 254, 284, 458, 1473
BssHII GCGCGC 1 cut(s) 298
BssMI GATC 4 cut(s) 52, 700, 1459, 1486
BssNI GRCGYC 1 cut(s) 446
BssT1I CCWWGG 3 cut(s) 185, 254, 1473
Bst2UI CCWGG 4 cut(s) 286, 565, 1152, 1322
Bst4CI ACNGT 4 cut(s) 22, 116, 659, 1176
BstACI GRCGYC 1 cut(s) 446
BstBI TTCGAA 1 cut(s) 480
BstC8I GCNNGC 2 cut(s) 300, 455
BstDEI CTNAG 7 cut(s) 410, 498, 630, 1050, 1145, 1335, 1482
BstENI CCTNNNNNAGG 1 cut(s) 1155
BstF5I GGATG 3 cut(s) 1009, 1296, 1447
BstFNI CGCG 2 cut(s) 300, 598
BstH2I RGCGCY 2 cut(s) 1145, 1429
BstHHI GCGC 4 cut(s) 300, 302, 1144, 1428
BstKTI GATC 4 cut(s) 55, 703, 1462, 1489
BstMBI GATC 4 cut(s) 52, 700, 1459, 1486
BstMCI CGRYCG 6 cut(s) 67, 106, 145, 214, 229, 602
BstMWI GCNNNNNNNGC 6 cut(s) 117, 126, 367, 400, 454, 1423
BstNI CCWGG 4 cut(s) 286, 565, 1152, 1322
BstSCI CCNGG 6 cut(s) 218, 284, 302, 563, 1150, 1320
BstSFI CTRYAG 1 cut(s) 1227
BstUI CGCG 2 cut(s) 300, 598
BstV1I GCAGC 3 cut(s) 132, 798, 1423
BstX2I RGATCY 1 cut(s) 1486
BstYI RGATCY 1 cut(s) 1486
BstZI CGGCCG 1 cut(s) 64
BtsCI GGATG 3 cut(s) 1009, 1296, 1447
BtsIMutI CAGTG 1 cut(s) 664
Cac8I GCNNGC 2 cut(s) 300, 455
CaiI CAGNNNCTG 2 cut(s) 409, 1417
CfoI GCGC 4 cut(s) 300, 302, 1144, 1428
Cfr10I RCCGGY 1 cut(s) 228
Cfr13I GGNCC 7 cut(s) 189, 216, 258, 269, 316, 1094, 1448
CseI GACGC 1 cut(s) 454
CsiI ACCWGGT 1 cut(s) 1320
Csp6I GTAC 3 cut(s) 139, 385, 901
CspAI ACCGGT 1 cut(s) 228
CviAII CATG 2 cut(s) 26, 1357
CviQI GTAC 3 cut(s) 139, 385, 901
DdeI CTNAG 7 cut(s) 410, 498, 630, 1050, 1145, 1335, 1482
DpnI GATC 4 cut(s) 54, 702, 1461, 1488
DpnII GATC 4 cut(s) 52, 700, 1459, 1486
EaeI YGGCCR 3 cut(s) 64, 332, 1353
EagI CGGCCG 1 cut(s) 64
EciI GGCGGA 1 cut(s) 796
EclXI CGGCCG 1 cut(s) 64
Eco130I CCWWGG 3 cut(s) 185, 254, 1473
Eco147I AGGCCT 4 cut(s) 10, 1022, 1150, 1478
Eco24I GRGCYC 1 cut(s) 455
Eco47I GGWCC 4 cut(s) 189, 258, 316, 1448
Eco47III AGCGCT 1 cut(s) 1143
Eco52I CGGCCG 1 cut(s) 64
Eco57I CTGAAG 3 cut(s) 383, 416, 972
EcoNI CCTNNNNNAGG 1 cut(s) 1155
EcoRI GAATTC 1 cut(s) 476
EcoRII CCWGG 4 cut(s) 284, 563, 1150, 1320
EcoT14I CCWWGG 3 cut(s) 185, 254, 1473
EcoT38I GRGCYC 1 cut(s) 455
ErhI CCWWGG 3 cut(s) 185, 254, 1473
FaeI CATG 2 cut(s) 29, 1360
FalI AAGNNNNNCTT 2 cut(s) 1206, 1238
FaqI GGGAC 1 cut(s) 329
FatI CATG 2 cut(s) 25, 1356
FauI CCCGC 2 cut(s) 84, 462
FbaI TGATCA 1 cut(s) 52
Fnu4HI GCNGC 5 cut(s) 109, 121, 787, 1412, 1415
FokI GGATG 3 cut(s) 1016, 1303, 1454
FriOI GRGCYC 1 cut(s) 455
Fsp4HI GCNGC 5 cut(s) 109, 121, 787, 1412, 1415
FspBI CTAG 7 cut(s) 180, 186, 255, 723, 729, 977, 1110
GlaI GCGC 4 cut(s) 299, 301, 1143, 1427
GluI GCNGC 5 cut(s) 109, 121, 787, 1412, 1415
GsaI CCCAGC 1 cut(s) 129
HaeII RGCGCY 2 cut(s) 1145, 1429
HapII CCGG 7 cut(s) 80, 219, 229, 272, 303, 319, 335
HgaI GACGC 1 cut(s) 454
HhaI GCGC 4 cut(s) 300, 302, 1144, 1428
Hin1I GRCGYC 1 cut(s) 446
Hin1II CATG 2 cut(s) 29, 1360
Hin6I GCGC 4 cut(s) 298, 300, 1142, 1426
HinP1I GCGC 4 cut(s) 298, 300, 1142, 1426
HpaII CCGG 7 cut(s) 80, 219, 229, 272, 303, 319, 335
HphI GGTGA 5 cut(s) 87, 533, 886, 1166, 1172
Hpy99I CGWCG 5 cut(s) 42, 104, 120, 249, 356
HpyCH4III ACNGT 4 cut(s) 22, 116, 659, 1176
HpyCH4V TGCA 3 cut(s) 1172, 1229, 1411
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 126, 367, 400, 454, 1423
HpyF3I CTNAG 7 cut(s) 410, 498, 630, 1050, 1145, 1335, 1482
Hsp92I GRCGYC 1 cut(s) 446
Hsp92II CATG 2 cut(s) 29, 1360
HspAI GCGC 4 cut(s) 298, 300, 1142, 1426
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 4 cut(s) 52, 700, 1459, 1486
LguI GCTCTTC 1 cut(s) 740
LmnI GCTCC 4 cut(s) 358, 783, 991, 1139
Lsp1109I GCAGC 3 cut(s) 132, 798, 1423
LweI GCATC 2 cut(s) 949, 1153
MabI ACCWGGT 1 cut(s) 1320
MaeI CTAG 7 cut(s) 180, 186, 255, 723, 729, 977, 1110
MaeIII GTNAC 2 cut(s) 68, 442
MalI GATC 4 cut(s) 54, 702, 1461, 1488
MboI GATC 4 cut(s) 52, 700, 1459, 1486
MflI RGATCY 1 cut(s) 1486
MhlI GDGCHC 2 cut(s) 455, 1392
MlsI TGGCCA 1 cut(s) 1355
MluCI AATT 4 cut(s) 476, 482, 548, 1028
MluNI TGGCCA 1 cut(s) 1355
MlyI GAGTC 4 cut(s) 284, 347, 356, 587
MmeI TCCRAC 5 cut(s) 216, 285, 321, 375, 561
Mox20I TGGCCA 1 cut(s) 1355
MscI TGGCCA 1 cut(s) 1355
MseI TTAA 1 cut(s) 1362
Msp20I TGGCCA 1 cut(s) 1355
MspA1I CMGCKG 1 cut(s) 1414
MspI CCGG 7 cut(s) 80, 219, 229, 272, 303, 319, 335
MspR9I CCNGG 6 cut(s) 220, 286, 304, 565, 1152, 1322
Mva1269I GAATGC 1 cut(s) 1300
MvaI CCWGG 4 cut(s) 286, 565, 1152, 1322
MvnI CGCG 2 cut(s) 300, 598
MwoI GCNNNNNNNGC 6 cut(s) 117, 126, 367, 400, 454, 1423
NciI CCSGG 2 cut(s) 220, 304
NdeII GATC 4 cut(s) 52, 700, 1459, 1486
NlaIII CATG 2 cut(s) 29, 1360
NlaIV GGNNCC 5 cut(s) 317, 993, 1058, 1283, 1488
NmeAIII GCCGAG 2 cut(s) 474, 483
NmuCI GTSAC 1 cut(s) 442
NspV TTCGAA 1 cut(s) 480
PalAI GGCGCGCC 1 cut(s) 298
PauI GCGCGC 1 cut(s) 298
PceI AGGCCT 4 cut(s) 10, 1022, 1150, 1478
PciSI GCTCTTC 1 cut(s) 740
PcsI WCGNNNNNNNCGW 3 cut(s) 43, 477, 540
PctI GAATGC 1 cut(s) 1300
PinAI ACCGGT 1 cut(s) 228
PkrI GCNGC 5 cut(s) 110, 122, 788, 1413, 1416
PleI GAGTC 4 cut(s) 283, 346, 355, 586
PpsI GAGTC 4 cut(s) 283, 346, 355, 586
Psp6I CCWGG 4 cut(s) 284, 563, 1150, 1320
PspFI CCCAGC 1 cut(s) 125
PspGI CCWGG 4 cut(s) 284, 563, 1150, 1320
PspN4I GGNNCC 5 cut(s) 317, 993, 1058, 1283, 1488
PspPI GGNCC 7 cut(s) 189, 216, 258, 269, 316, 1094, 1448
PstI CTGCAG 1 cut(s) 1231
PstNI CAGNNNCTG 2 cut(s) 409, 1417
PsuI RGATCY 1 cut(s) 1486
PteI GCGCGC 1 cut(s) 298
RsaI GTAC 3 cut(s) 140, 386, 902
RsaNI GTAC 3 cut(s) 139, 385, 901
SapI GCTCTTC 1 cut(s) 740
SaqAI TTAA 1 cut(s) 1362
SatI GCNGC 5 cut(s) 109, 121, 787, 1412, 1415
Sau3AI GATC 4 cut(s) 52, 700, 1459, 1486
Sau96I GGNCC 7 cut(s) 189, 216, 258, 269, 316, 1094, 1448
ScaI AGTACT 1 cut(s) 902
SchI GAGTC 4 cut(s) 284, 347, 356, 587
ScrFI CCNGG 6 cut(s) 220, 286, 304, 565, 1152, 1322
SduI GDGCHC 2 cut(s) 455, 1392
SexAI ACCWGGT 1 cut(s) 1320
SfaNI GCATC 2 cut(s) 949, 1153
SfcI CTRYAG 1 cut(s) 1227
SfuI TTCGAA 1 cut(s) 480
SgsI GGCGCGCC 1 cut(s) 298
SinI GGWCC 4 cut(s) 189, 258, 316, 1448
SmlI CTYRAG 2 cut(s) 377, 1043
SmoI CTYRAG 2 cut(s) 377, 1043
Sse9I AATT 4 cut(s) 476, 482, 548, 1028
SseBI AGGCCT 4 cut(s) 10, 1022, 1150, 1478
SsiI CCGC 8 cut(s) 77, 106, 109, 145, 455, 598, 781, 1414
SspMI CTAG 7 cut(s) 180, 186, 255, 723, 729, 977, 1110
StuI AGGCCT 4 cut(s) 10, 1022, 1150, 1478
StyD4I CCNGG 6 cut(s) 218, 284, 302, 563, 1150, 1320
StyI CCWWGG 3 cut(s) 185, 254, 1473
TaaI ACNGT 4 cut(s) 22, 116, 659, 1176
TaqI TCGA 7 cut(s) 37, 244, 480, 602, 650, 854, 981
TaqII GACCGA 1 cut(s) 200
TasI AATT 4 cut(s) 476, 482, 548, 1028
TatI WGTACW 1 cut(s) 900
TauI GCSGC 2 cut(s) 111, 1417
Tru1I TTAA 1 cut(s) 1362
Tru9I TTAA 1 cut(s) 1362
TscAI CASTG 1 cut(s) 664
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 3 cut(s) 120, 786, 1411
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 5 cut(s) 14, 147, 489, 1095, 1307
TspGWI ACGGA 4 cut(s) 35, 210, 279, 926
TspRI CASTG 1 cut(s) 664
VpaK11BI GGWCC 4 cut(s) 189, 258, 316, 1448
XagI CCTNNNNNAGG 1 cut(s) 1155
XapI RAATTY 1 cut(s) 476
XbaI TCTAGA 1 cut(s) 722
XmaJI CCTAGG 2 cut(s) 185, 254
XspI CTAG 7 cut(s) 180, 186, 255, 723, 729, 977, 1110
ZrmI AGTACT 1 cut(s) 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.