Rw0G003210

Ras-induced vulval development antagonist

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00109
OG0008597
Physical Location & Seq
Reverse (-)
28956 .. 30470
1515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G003210.1

Sequence Viewer

Length: 1515 bp
ATGGCGAGGCCTTCTTCCACCGTTCATGCCCCCAATCGACGATTCCGTTCTGATCACGATAACAGCCGTTACTCCCCGCCGGACTATGACAACTCACCTGACGACCGCCGCCACCGTCGCAGCCCCAGCTATGAAAGGTACGACCGCCACCGAAATCGCTCTTACTCTCCTGATTACCCTAGCCCTAGGTCCAACGGAGGCCGAAGATTCGGTCGGGCCGGGAACGACCGGTCTTACTCGCCTCGACGAAGCCCTAGGTCCAACGGAGGGCCGGAGTCTTTACCCAGGAGATTCGGGCGCGCCGGGAACGATAGGGACCGGAATGGAAGGTGGCCGGAGTCGGAGGAGTCCGACGAGGAGCTGAAGGGGCTGAATATTGAGGAGTACCGAAGGCTGAAGAGGCAGAAGCTGAGGAAGGCGTTGAGGAGTTGTATCTGGAATGTGACGCCGAGCCCGCCGAGGAATGAGAACGATGAATTCGAATTGGAGGACAAGGCTGACGAGATTCCAGAGGTTCTTGGTGAAGAGAACAACGATTCAGGCGAGAAATTGAAGGAAAAAGCCAAGAGTGAATCGGAGTCTGAATCGGAATCGGAGGATTCGCGGTCGAGAAAGAGGAAGAAGAGTTCGGGTTCTAAGCGTAGGAAGAAGAGTTCGAGATACAGTGATAGCGAATCGGAGAGCGAGAGTGATGGTGAGTCAGATGAGGAAGAGGATCACAGAAGGAGAAGGAAATCTAGAACCAGGAGTAAGCGAAAGAAGAGCAGAAGAGAAAGGAGAGGAAGGAAGAGTAGGCGGAGCAGCGATTCCGATGAGAGTGAAGCAAGTGATAGTGAGGAGGGTTCAGATTCTTCTGATAGGGTAAAGTCGAAGAAGAAGAAGGGAAAGGTGAGCAGTAAGAGTAAGAAGAAGTACACTGAAACGGAGAGTGAGGATTCTGGTTCAGAGAAGAGTTTGGATTCTGAAGTTGATGCCAAGCCTAAAGCTCTAGTCGAAGTGGAGGAGCCAGAAATGGATGAGAATAGTATTGAGGCCTTGAAATTGAAGGAAATCCTTGAGGCTCAGAGGAACCCTTCTTTGGATTTGGATAATGAACCTGTGGTTGGGCCAATGCCCTTGCCTAGAGCCGAAGGGCATATTAGTTATGGTGGAGCGCTTAGGCCTGGTGAAGGTGATGCCATTGCACAGTATGTTCAACAAGGGAAGCGTATTCCACGAAGAGGAGAAGTTGGTCTTTCTGCAGAGGAGATTCAGAAGTTTGAGGGTCTTGGCTATGTGATGAGTGGTAGTAGGCACCAGAGGATGAATGCCATTCGTATTAGAAAGGAAAACCAGGTTTATAGTGCTGAGGATAAGAGGGCATTGGCCATGTTTAACTATGAGGAGAAAGCAAAGCGTGAGCACAAGGTTATGGCTGATTTGCAGCGACTGGTTCAGCGCCATATTGGACAGGATGTTGGTCCTACTCATGATCCCTTTTCTTCCAAGGCCTCCGAGGATCCTGATGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SynMuv_product

Protein Analysis

504

Amino Acids

57.89

Weight (kDa)

9.09

Isoelectric Point (pI)

90.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nkap_C PF06047 381 - 479 9.5e-47 NF-kappa-B-activating protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1293
AccII CGCG 2 cut(s) 300, 604
AciI CCGC 7 cut(s) 77, 106, 109, 145, 455, 604, 796
AclWI GGATC 4 cut(s) 723, 1466, 1493, 1506
AcoI YGGCCR 2 cut(s) 332, 1365
AcsI RAATTY 1 cut(s) 476
AcuI CTGAAG 3 cut(s) 383, 416, 984
AcyI GRCGYC 1 cut(s) 446
AfaI GTAC 3 cut(s) 140, 386, 914
AfeI AGCGCT 1 cut(s) 1155
AfiI CCNNNNNNNGG 5 cut(s) 267, 1078, 1103, 1169, 1220
AgeI ACCGGT 1 cut(s) 228
AgsI TTSAA 4 cut(s) 553, 1039, 1045, 1196
AjnI CCWGG 4 cut(s) 284, 743, 1162, 1332
AjuI GAANNNNNNNTTGG 6 cut(s) 26, 58, 1061, 1086, 1093, 1118
AloI GAACNNNNNNTCC 4 cut(s) 610, 637, 642, 669
AluBI AGCT 4 cut(s) 129, 361, 409, 986
AluI AGCT 4 cut(s) 129, 361, 409, 986
Alw21I GWGCWC 1 cut(s) 1404
AlwI GGATC 4 cut(s) 723, 1466, 1493, 1506
AlwNI CAGNNNCTG 2 cut(s) 409, 1429
Aor51HI AGCGCT 1 cut(s) 1155
ApeKI GCWGC 3 cut(s) 120, 801, 1423
ApoI RAATTY 1 cut(s) 476
ArsI GACNNNNNNTTYG 2 cut(s) 196, 228
AscI GGCGCGCC 1 cut(s) 298
AsiGI ACCGGT 1 cut(s) 228
AspA2I CCTAGG 2 cut(s) 185, 254
AspLEI GCGC 4 cut(s) 300, 302, 1156, 1440
AspS9I GGNCC 7 cut(s) 189, 216, 258, 269, 316, 1106, 1460
AsuC2I CCSGG 2 cut(s) 220, 304
AsuHPI GGTGA 6 cut(s) 87, 533, 707, 901, 1178, 1184
AsuII TTCGAA 1 cut(s) 480
AvaII GGWCC 4 cut(s) 189, 258, 316, 1460
AvrII CCTAGG 2 cut(s) 185, 254
BalI TGGCCA 1 cut(s) 1367
BamHI GGATCC 1 cut(s) 1498
BanI GGYRCC 1 cut(s) 1293
BanII GRGCYC 1 cut(s) 455
Bbv12I GWGCWC 1 cut(s) 1404
BbvCI CCTCAGC 2 cut(s) 410, 1347
BbvI GCAGC 3 cut(s) 132, 813, 1435
BccI CCATC 1 cut(s) 686
BceAI ACGGC 1 cut(s) 51
BciT130I CCWGG 4 cut(s) 286, 745, 1164, 1334
BclI TGATCA 1 cut(s) 52
BcnI CCSGG 2 cut(s) 220, 304
BfaI CTAG 6 cut(s) 180, 186, 255, 738, 989, 1122
BfmI CTRYAG 1 cut(s) 1239
BfoI RGCGCY 2 cut(s) 1157, 1441
BisI GCNGC 4 cut(s) 109, 121, 802, 1424
BlnI CCTAGG 2 cut(s) 185, 254
BlsI GCNGC 4 cut(s) 110, 122, 803, 1425
Bme1390I CCNGG 6 cut(s) 220, 286, 304, 745, 1164, 1334
Bme18I GGWCC 4 cut(s) 189, 258, 316, 1460
BmgT120I GGNCC 7 cut(s) 189, 216, 258, 269, 316, 1106, 1460
BmiI GGNNCC 5 cut(s) 317, 1005, 1070, 1295, 1500
BmrFI CCNGG 6 cut(s) 220, 286, 304, 745, 1164, 1334
BmsI GCATC 2 cut(s) 961, 1165
Bpu10I CCTNAGC 3 cut(s) 410, 1157, 1347
Bpu14I TTCGAA 1 cut(s) 480
BpuEI CTTGAG 1 cut(s) 1076
BpuMI CCSGG 2 cut(s) 220, 304
BsaHI GRCGYC 1 cut(s) 446
BsaJI CCNNGG 6 cut(s) 185, 254, 284, 458, 1485, 1494
BsaWI WCCGGW 2 cut(s) 228, 318
Bsc4I CCNNNNNNNGG 5 cut(s) 267, 1078, 1103, 1169, 1220
Bse118I RCCGGY 1 cut(s) 228
Bse1I ACTGG 1 cut(s) 1434
Bse3DI GCAATG 1 cut(s) 1179
BseBI CCWGG 4 cut(s) 286, 745, 1164, 1334
BseDI CCNNGG 6 cut(s) 185, 254, 284, 458, 1485, 1494
BseGI GGATG 3 cut(s) 1021, 1308, 1459
BseLI CCNNNNNNNGG 5 cut(s) 267, 1078, 1103, 1169, 1220
BseMI GCAATG 1 cut(s) 1179
BseMII CTCAG 3 cut(s) 401, 1076, 1338
BseNI ACTGG 1 cut(s) 1434
BsePI GCGCGC 1 cut(s) 298
BseRI GAGGAG 9 cut(s) 359, 371, 395, 439, 851, 1016, 1236, 1259, 1397
BseXI GCAGC 3 cut(s) 132, 813, 1435
BseYI CCCAGC 1 cut(s) 125
Bsh1236I CGCG 2 cut(s) 300, 604
Bsh1285I CGRYCG 5 cut(s) 106, 145, 214, 229, 608
BshNI GGYRCC 1 cut(s) 1293
BshTI ACCGGT 1 cut(s) 228
BsiEI CGRYCG 5 cut(s) 106, 145, 214, 229, 608
BsiHKAI GWGCWC 1 cut(s) 1404
BsiSI CCGG 7 cut(s) 80, 219, 229, 272, 303, 319, 335
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 5 cut(s) 267, 1078, 1103, 1169, 1220
BsmFI GGGAC 1 cut(s) 329
BsmI GAATGC 1 cut(s) 1312
Bsp119I TTCGAA 1 cut(s) 480
Bsp1286I GDGCHC 2 cut(s) 455, 1404
Bsp143I GATC 4 cut(s) 52, 715, 1471, 1498
BspACI CCGC 7 cut(s) 77, 106, 109, 145, 455, 604, 796
BspCNI CTCAG 3 cut(s) 402, 1075, 1339
BspFNI CGCG 2 cut(s) 300, 604
BspHI TCATGA 1 cut(s) 1468
BspLI GGNNCC 5 cut(s) 317, 1005, 1070, 1295, 1500
BspMAI CTGCAG 1 cut(s) 1243
BspPI GGATC 4 cut(s) 723, 1466, 1493, 1506
BspQI GCTCTTC 1 cut(s) 755
BspT104I TTCGAA 1 cut(s) 480
BspT107I GGYRCC 1 cut(s) 1293
BsrDI GCAATG 1 cut(s) 1179
BsrFI RCCGGY 1 cut(s) 228
BsrI ACTGG 1 cut(s) 1434
BssAI RCCGGY 1 cut(s) 228
BssECI CCNNGG 6 cut(s) 185, 254, 284, 458, 1485, 1494
BssHII GCGCGC 1 cut(s) 298
BssMI GATC 4 cut(s) 52, 715, 1471, 1498
BssNI GRCGYC 1 cut(s) 446
BssT1I CCWWGG 3 cut(s) 185, 254, 1485
Bst2UI CCWGG 4 cut(s) 286, 745, 1164, 1334
Bst4CI ACNGT 4 cut(s) 22, 116, 665, 1188
BstACI GRCGYC 1 cut(s) 446
BstBI TTCGAA 1 cut(s) 480
BstC8I GCNNGC 2 cut(s) 300, 455
BstDEI CTNAG 5 cut(s) 410, 636, 1062, 1157, 1347
BstENI CCTNNNNNAGG 1 cut(s) 1167
BstF5I GGATG 3 cut(s) 1021, 1308, 1459
BstFNI CGCG 2 cut(s) 300, 604
BstH2I RGCGCY 2 cut(s) 1157, 1441
BstHHI GCGC 4 cut(s) 300, 302, 1156, 1440
BstKTI GATC 4 cut(s) 55, 718, 1474, 1501
BstMBI GATC 4 cut(s) 52, 715, 1471, 1498
BstMCI CGRYCG 5 cut(s) 106, 145, 214, 229, 608
BstMWI GCNNNNNNNGC 5 cut(s) 117, 126, 367, 400, 454
BstNI CCWGG 4 cut(s) 286, 745, 1164, 1334
BstSCI CCNGG 6 cut(s) 218, 284, 302, 743, 1162, 1332
BstSFI CTRYAG 1 cut(s) 1239
BstUI CGCG 2 cut(s) 300, 604
BstV1I GCAGC 3 cut(s) 132, 813, 1435
BstX2I RGATCY 1 cut(s) 1498
BstYI RGATCY 1 cut(s) 1498
BtsCI GGATG 3 cut(s) 1021, 1308, 1459
BtsIMutI CAGTG 2 cut(s) 670, 915
Cac8I GCNNGC 2 cut(s) 300, 455
CaiI CAGNNNCTG 2 cut(s) 409, 1429
CciI TCATGA 1 cut(s) 1468
CfoI GCGC 4 cut(s) 300, 302, 1156, 1440
Cfr10I RCCGGY 1 cut(s) 228
Cfr13I GGNCC 7 cut(s) 189, 216, 258, 269, 316, 1106, 1460
CseI GACGC 1 cut(s) 454
CsiI ACCWGGT 1 cut(s) 1332
Csp6I GTAC 3 cut(s) 139, 385, 913
CspAI ACCGGT 1 cut(s) 228
CviAII CATG 3 cut(s) 26, 1369, 1469
CviQI GTAC 3 cut(s) 139, 385, 913
DdeI CTNAG 5 cut(s) 410, 636, 1062, 1157, 1347
DpnI GATC 4 cut(s) 54, 717, 1473, 1500
DpnII GATC 4 cut(s) 52, 715, 1471, 1498
EaeI YGGCCR 2 cut(s) 332, 1365
EciI GGCGGA 1 cut(s) 811
Eco130I CCWWGG 3 cut(s) 185, 254, 1485
Eco147I AGGCCT 4 cut(s) 10, 1034, 1162, 1490
Eco24I GRGCYC 1 cut(s) 455
Eco47I GGWCC 4 cut(s) 189, 258, 316, 1460
Eco47III AGCGCT 1 cut(s) 1155
Eco57I CTGAAG 3 cut(s) 383, 416, 984
EcoNI CCTNNNNNAGG 1 cut(s) 1167
EcoRI GAATTC 1 cut(s) 476
EcoRII CCWGG 4 cut(s) 284, 743, 1162, 1332
EcoT14I CCWWGG 3 cut(s) 185, 254, 1485
EcoT38I GRGCYC 1 cut(s) 455
ErhI CCWWGG 3 cut(s) 185, 254, 1485
FaeI CATG 3 cut(s) 29, 1372, 1472
FalI AAGNNNNNCTT 2 cut(s) 1218, 1250
FaqI GGGAC 1 cut(s) 329
FatI CATG 3 cut(s) 25, 1368, 1468
FauI CCCGC 2 cut(s) 84, 462
FbaI TGATCA 1 cut(s) 52
Fnu4HI GCNGC 4 cut(s) 109, 121, 802, 1424
FokI GGATG 3 cut(s) 1028, 1315, 1466
FriOI GRGCYC 1 cut(s) 455
Fsp4HI GCNGC 4 cut(s) 109, 121, 802, 1424
FspBI CTAG 6 cut(s) 180, 186, 255, 738, 989, 1122
GlaI GCGC 4 cut(s) 299, 301, 1155, 1439
GluI GCNGC 4 cut(s) 109, 121, 802, 1424
GsaI CCCAGC 1 cut(s) 129
HaeII RGCGCY 2 cut(s) 1157, 1441
HapII CCGG 7 cut(s) 80, 219, 229, 272, 303, 319, 335
HgaI GACGC 1 cut(s) 454
HhaI GCGC 4 cut(s) 300, 302, 1156, 1440
Hin1I GRCGYC 1 cut(s) 446
Hin1II CATG 3 cut(s) 29, 1372, 1472
Hin6I GCGC 4 cut(s) 298, 300, 1154, 1438
HinP1I GCGC 4 cut(s) 298, 300, 1154, 1438
HpaII CCGG 7 cut(s) 80, 219, 229, 272, 303, 319, 335
HphI GGTGA 6 cut(s) 87, 533, 707, 901, 1178, 1184
Hpy166II GTNNAC 1 cut(s) 915
Hpy188III TCNNGA 9 cut(s) 56, 170, 436, 509, 609, 657, 738, 1469, 1502
Hpy8I GTNNAC 1 cut(s) 915
Hpy99I CGWCG 4 cut(s) 42, 120, 249, 356
HpyCH4III ACNGT 4 cut(s) 22, 116, 665, 1188
HpyCH4V TGCA 3 cut(s) 1184, 1241, 1423
HpyF10VI GCNNNNNNNGC 5 cut(s) 117, 126, 367, 400, 454
HpyF3I CTNAG 5 cut(s) 410, 636, 1062, 1157, 1347
Hsp92I GRCGYC 1 cut(s) 446
Hsp92II CATG 3 cut(s) 29, 1372, 1472
HspAI GCGC 4 cut(s) 298, 300, 1154, 1438
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 4 cut(s) 52, 715, 1471, 1498
LguI GCTCTTC 1 cut(s) 755
LmnI GCTCC 4 cut(s) 358, 798, 1003, 1151
Lsp1109I GCAGC 3 cut(s) 132, 813, 1435
LweI GCATC 2 cut(s) 961, 1165
MabI ACCWGGT 1 cut(s) 1332
MaeI CTAG 6 cut(s) 180, 186, 255, 738, 989, 1122
MaeIII GTNAC 2 cut(s) 68, 442
MalI GATC 4 cut(s) 54, 717, 1473, 1500
MboI GATC 4 cut(s) 52, 715, 1471, 1498
MflI RGATCY 1 cut(s) 1498
MhlI GDGCHC 2 cut(s) 455, 1404
MlsI TGGCCA 1 cut(s) 1367
MluCI AATT 4 cut(s) 476, 482, 548, 1040
MluNI TGGCCA 1 cut(s) 1367
MlyI GAGTC 5 cut(s) 284, 347, 356, 587, 707
MmeI TCCRAC 4 cut(s) 216, 285, 321, 375
Mox20I TGGCCA 1 cut(s) 1367
MscI TGGCCA 1 cut(s) 1367
MseI TTAA 1 cut(s) 1374
Msp20I TGGCCA 1 cut(s) 1367
MspI CCGG 7 cut(s) 80, 219, 229, 272, 303, 319, 335
MspR9I CCNGG 6 cut(s) 220, 286, 304, 745, 1164, 1334
Mva1269I GAATGC 1 cut(s) 1312
MvaI CCWGG 4 cut(s) 286, 745, 1164, 1334
MvnI CGCG 2 cut(s) 300, 604
MwoI GCNNNNNNNGC 5 cut(s) 117, 126, 367, 400, 454
NciI CCSGG 2 cut(s) 220, 304
NdeII GATC 4 cut(s) 52, 715, 1471, 1498
NlaIII CATG 3 cut(s) 29, 1372, 1472
NlaIV GGNNCC 5 cut(s) 317, 1005, 1070, 1295, 1500
NmeAIII GCCGAG 2 cut(s) 474, 483
NmuCI GTSAC 1 cut(s) 442
NspV TTCGAA 1 cut(s) 480
PagI TCATGA 1 cut(s) 1468
PalAI GGCGCGCC 1 cut(s) 298
PauI GCGCGC 1 cut(s) 298
PceI AGGCCT 4 cut(s) 10, 1034, 1162, 1490
PciSI GCTCTTC 1 cut(s) 755
PcsI WCGNNNNNNNCGW 3 cut(s) 43, 477, 540
PctI GAATGC 1 cut(s) 1312
PinAI ACCGGT 1 cut(s) 228
PkrI GCNGC 4 cut(s) 110, 122, 803, 1425
PleI GAGTC 5 cut(s) 283, 346, 355, 586, 706
PpsI GAGTC 5 cut(s) 283, 346, 355, 586, 706
Psp6I CCWGG 4 cut(s) 284, 743, 1162, 1332
PspFI CCCAGC 1 cut(s) 125
PspGI CCWGG 4 cut(s) 284, 743, 1162, 1332
PspN4I GGNNCC 5 cut(s) 317, 1005, 1070, 1295, 1500
PspPI GGNCC 7 cut(s) 189, 216, 258, 269, 316, 1106, 1460
PstI CTGCAG 1 cut(s) 1243
PstNI CAGNNNCTG 2 cut(s) 409, 1429
PsuI RGATCY 1 cut(s) 1498
PteI GCGCGC 1 cut(s) 298
RsaI GTAC 3 cut(s) 140, 386, 914
RsaNI GTAC 3 cut(s) 139, 385, 913
SapI GCTCTTC 1 cut(s) 755
SaqAI TTAA 1 cut(s) 1374
SatI GCNGC 4 cut(s) 109, 121, 802, 1424
Sau3AI GATC 4 cut(s) 52, 715, 1471, 1498
Sau96I GGNCC 7 cut(s) 189, 216, 258, 269, 316, 1106, 1460
SchI GAGTC 5 cut(s) 284, 347, 356, 587, 707
ScrFI CCNGG 6 cut(s) 220, 286, 304, 745, 1164, 1334
SduI GDGCHC 2 cut(s) 455, 1404
SexAI ACCWGGT 1 cut(s) 1332
SfaNI GCATC 2 cut(s) 961, 1165
SfcI CTRYAG 1 cut(s) 1239
SfuI TTCGAA 1 cut(s) 480
SgsI GGCGCGCC 1 cut(s) 298
SinI GGWCC 4 cut(s) 189, 258, 316, 1460
SmlI CTYRAG 1 cut(s) 1055
SmoI CTYRAG 1 cut(s) 1055
Sse9I AATT 4 cut(s) 476, 482, 548, 1040
SseBI AGGCCT 4 cut(s) 10, 1034, 1162, 1490
SsiI CCGC 7 cut(s) 77, 106, 109, 145, 455, 604, 796
SspI AATATT 1 cut(s) 376
SspMI CTAG 6 cut(s) 180, 186, 255, 738, 989, 1122
StuI AGGCCT 4 cut(s) 10, 1034, 1162, 1490
StyD4I CCNGG 6 cut(s) 218, 284, 302, 743, 1162, 1332
StyI CCWWGG 3 cut(s) 185, 254, 1485
TaaI ACNGT 4 cut(s) 22, 116, 665, 1188
TaqI TCGA 7 cut(s) 37, 244, 480, 608, 656, 869, 993
TaqII GACCGA 1 cut(s) 200
TasI AATT 4 cut(s) 476, 482, 548, 1040
TatI WGTACW 1 cut(s) 912
TauI GCSGC 1 cut(s) 111
Tru1I TTAA 1 cut(s) 1374
Tru9I TTAA 1 cut(s) 1374
TscAI CASTG 2 cut(s) 670, 922
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 3 cut(s) 120, 801, 1423
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 5 cut(s) 14, 147, 489, 1107, 1319
TspGWI ACGGA 4 cut(s) 35, 210, 279, 938
TspRI CASTG 2 cut(s) 670, 922
VpaK11BI GGWCC 4 cut(s) 189, 258, 316, 1460
XagI CCTNNNNNAGG 1 cut(s) 1167
XapI RAATTY 1 cut(s) 476
XbaI TCTAGA 1 cut(s) 737
XmaJI CCTAGG 2 cut(s) 185, 254
XspI CTAG 6 cut(s) 180, 186, 255, 738, 989, 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.