RLG00000009424

tRNA pseudouridine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0006106
Physical Location & Seq
Reverse (-)
52655769 .. 52659830
4062 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009424

Sequence Viewer

Length: 1233 bp
ATGGAGAGCAAGGTCTTTGTCCAATACAACCACACCGATCCCTGTGCATTTGCGCGCTGGACTACCAAGGAAAGCTTCCAGTTCATGTCAGCCAGGCCTTGGCAACAAGTCATCGACTTTTATTCCGACGTGGTAAAGGGTCAGAAGCCCTTGTCTGCGTTGTTGGGTACTACTTGTTTGAGTAAAAACAGAATTTACAATAGTATAGAGAGTGGAAATTCTCAAAATAGGGGTGGGAGGTGGGCAAGATTGACGTTCAAGATTGTGGTTTCGTATCATGGGGGTTCCTTTGATGGGTGGCAAAAGCAGCCGGACCTCTACACTGTCCAAAGTGTTGTTGAACAATCTCTTGGGAAATTTGTTGATGACAAGAAAGCCCAGATGCTGAAAGACAAAGGGTTGCCATTGGAAGGGGCTGCTACTGTCGCCGGACGGACCGATAAAGGAGTGACAGCCCTTAATCAAGTTTGTTCTTTCTATACTTGGAGAAAGGATGTGCAATCTCAAGATATTGCAGATGCTATAAATATTGCAGCACCAGGAAAACTCAGGACAGTATCAGTTACTGAGGTATCACGTGTATTCCATCCAAATTTTTCTGCCAAATGGAGGCGTTATTTGTATATATTTCCATTTAATGATGATGAGGAGGACAGGGAACAAAGCAATGAGACTGGAGAGAATACTCAAAAATTGAAAATTAAAGATAATCATTATGAGCATGAAGTTGTATCTGATAAATGCAATGAGGAAAATGTTGGAAACTTAATCATCACTGATGATGTAGAGTTTGAGACTGAGAAGAAGCCAAGGATGTTCAGCGTAAGCAGGGTGAACAAACTTTTACAGAAACTAGAAGGAAAGTTATTATCATATAAGCTATTTGCACGCGATACCAAAGCATCCAGAAATGAGGGTCCACCAACAGAGTGTTTTGTCTTCCATGCTCGAGCAAGAGAGTACAGATTACCGTGTTCAGAGCATGAAAAAGGGAGAAGGGTCATGTGTATTGAACTGGTTGCCAACCGATTCTTACGCAGGATGGTTCGGGTTCTTGTGGCAACCTCAATTAGGGAAGCCGCTGCAGGTGCAGATGAGGACGCATTGCTAAAACTGATGGATGCAACATGCAGACGTGCTACTGCTCCCCCAGCTCCACCCGATGGTTTAAGCCTTGTTGATGTTGGTTATGAAGAATTCGATCCGCTAGATTGCCTTATCTCGAAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PseudoU_synth_1

Protein Analysis

411

Amino Acids

46.61

Weight (kDa)

7.98

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_1 PF01416 332 - 398 3.1e-07 tRNA pseudouridine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1076
Acc36I ACCTGC 1 cut(s) 1076
AccB7I CCANNNNNTGG 2 cut(s) 99, 1163
AccII CGCG 2 cut(s) 55, 891
AciI CCGC 2 cut(s) 1080, 1205
AclWI GGATC 2 cut(s) 32, 1196
AcsI RAATTY 5 cut(s) 192, 217, 356, 592, 1196
AcvI CACGTG 1 cut(s) 578
AfaI GTAC 2 cut(s) 169, 962
AfiI CCNNNNNNNGG 6 cut(s) 99, 294, 410, 609, 1071, 1163
AflIII ACRYGT 1 cut(s) 577
AgsI TTSAA 4 cut(s) 259, 341, 697, 1013
AjiI CACGTC 2 cut(s) 130, 1136
AjnI CCWGG 2 cut(s) 92, 538
AjuI GAANNNNNNNTTGG 4 cut(s) 59, 91, 333, 365
AluBI AGCT 3 cut(s) 75, 880, 1154
AluI AGCT 3 cut(s) 75, 880, 1154
Alw26I GTCTC 2 cut(s) 665, 788
AlwI GGATC 2 cut(s) 32, 1196
AlwNI CAGNNNCTG 2 cut(s) 385, 566
Ama87I CYCGRG 1 cut(s) 948
AoxI GGCC 1 cut(s) 95
ApeKI GCWGC 4 cut(s) 307, 416, 533, 1082
ApoI RAATTY 5 cut(s) 192, 217, 356, 592, 1196
AspLEI GCGC 2 cut(s) 55, 57
AspS9I GGNCC 3 cut(s) 313, 435, 917
AsuHPI GGTGA 1 cut(s) 844
AvaI CYCGRG 1 cut(s) 948
AvaII GGWCC 3 cut(s) 313, 435, 917
BaeI ACNNNNGTAYC 2 cut(s) 555, 588
BbrPI CACGTG 1 cut(s) 578
BbsI GAAGAC 1 cut(s) 931
BbvI GCAGC 4 cut(s) 319, 403, 545, 1069
BccI CCATC 5 cut(s) 287, 594, 1036, 1111, 1157
BcgI CGANNNNNNTGC 2 cut(s) 26, 60
BciT130I CCWGG 2 cut(s) 94, 540
BcoDI GTCTC 2 cut(s) 665, 788
BfaI CTAG 2 cut(s) 854, 1208
BfmI CTRYAG 1 cut(s) 1083
BfuAI ACCTGC 1 cut(s) 1076
BisI GCNGC 5 cut(s) 308, 417, 534, 1080, 1083
BlsI GCNGC 5 cut(s) 309, 418, 535, 1081, 1084
Bme1390I CCNGG 2 cut(s) 94, 540
Bme18I GGWCC 3 cut(s) 313, 435, 917
BmeT110I CYCGRG 1 cut(s) 948
BmgBI CACGTC 2 cut(s) 130, 1136
BmgT120I GGNCC 3 cut(s) 313, 435, 917
BmiI GGNNCC 2 cut(s) 286, 918
BmrFI CCNGG 2 cut(s) 94, 540
BmsI GCATC 4 cut(s) 372, 508, 911, 1111
BpiI GAAGAC 1 cut(s) 931
BpmI CTGGAG 1 cut(s) 696
BpuEI CTTGAG 1 cut(s) 489
BsaAI YACGTR 1 cut(s) 578
BsaJI CCNNGG 3 cut(s) 66, 98, 809
Bsc4I CCNNNNNNNGG 6 cut(s) 99, 294, 410, 609, 1071, 1163
Bse1I ACTGG 3 cut(s) 79, 679, 1020
Bse3DI GCAATG 3 cut(s) 673, 751, 1103
BseBI CCWGG 2 cut(s) 94, 540
BseDI CCNNGG 3 cut(s) 66, 98, 809
BseGI GGATG 6 cut(s) 499, 586, 819, 902, 1047, 1126
BseLI CCNNNNNNNGG 6 cut(s) 99, 294, 410, 609, 1071, 1163
BseMI GCAATG 3 cut(s) 673, 751, 1103
BseMII CTCAG 3 cut(s) 558, 562, 789
BseNI ACTGG 3 cut(s) 79, 679, 1020
BsePI GCGCGC 1 cut(s) 53
BseRI GAGGAG 1 cut(s) 662
BseXI GCAGC 4 cut(s) 319, 403, 545, 1069
BseYI CCCAGC 1 cut(s) 1150
BsgI GTGCAG 1 cut(s) 1110
Bsh1236I CGCG 2 cut(s) 55, 891
BshFI GGCC 1 cut(s) 97
BsiHKCI CYCGRG 1 cut(s) 948
BsiSI CCGG 2 cut(s) 311, 429
BslI CCNNNNNNNGG 6 cut(s) 99, 294, 410, 609, 1071, 1163
BsmAI GTCTC 2 cut(s) 665, 788
BsnI GGCC 1 cut(s) 97
BsoBI CYCGRG 1 cut(s) 948
Bsp143I GATC 2 cut(s) 37, 1201
BspACI CCGC 2 cut(s) 1080, 1205
BspANI GGCC 1 cut(s) 97
BspCNI CTCAG 3 cut(s) 559, 561, 790
BspFNI CGCG 2 cut(s) 55, 891
BspLI GGNNCC 2 cut(s) 286, 918
BspMAI CTGCAG 1 cut(s) 1087
BspMI ACCTGC 1 cut(s) 1076
BspPI GGATC 2 cut(s) 32, 1196
BsrDI GCAATG 3 cut(s) 673, 751, 1103
BsrI ACTGG 3 cut(s) 79, 679, 1020
BssECI CCNNGG 3 cut(s) 66, 98, 809
BssHII GCGCGC 1 cut(s) 53
BssMI GATC 2 cut(s) 37, 1201
BssT1I CCWWGG 3 cut(s) 66, 98, 809
Bst2UI CCWGG 2 cut(s) 94, 540
Bst4CI ACNGT 4 cut(s) 325, 424, 556, 972
BstBAI YACGTR 1 cut(s) 578
BstC8I GCNNGC 2 cut(s) 55, 889
BstDEI CTNAG 3 cut(s) 548, 567, 798
BstENI CCTNNNNNAGG 1 cut(s) 1069
BstF5I GGATG 6 cut(s) 499, 586, 819, 902, 1047, 1126
BstFNI CGCG 2 cut(s) 55, 891
BstHHI GCGC 2 cut(s) 55, 57
BstKTI GATC 2 cut(s) 40, 1204
BstMAI GTCTC 2 cut(s) 665, 788
BstMBI GATC 2 cut(s) 37, 1201
BstMWI GCNNNNNNNGC 4 cut(s) 307, 425, 1088, 1151
BstNI CCWGG 2 cut(s) 94, 540
BstNSI RCATGY 1 cut(s) 1131
BstSCI CCNGG 2 cut(s) 92, 538
BstSFI CTRYAG 1 cut(s) 1083
BstUI CGCG 2 cut(s) 55, 891
BstV1I GCAGC 4 cut(s) 319, 403, 545, 1069
BstV2I GAAGAC 1 cut(s) 931
BsuRI GGCC 1 cut(s) 97
BtrI CACGTC 2 cut(s) 130, 1136
BtsCI GGATG 6 cut(s) 499, 586, 819, 902, 1047, 1126
BtsIMutI CAGTG 2 cut(s) 321, 774
BveI ACCTGC 1 cut(s) 1076
Cac8I GCNNGC 2 cut(s) 55, 889
CaiI CAGNNNCTG 2 cut(s) 385, 566
CfoI GCGC 2 cut(s) 55, 57
Cfr13I GGNCC 3 cut(s) 313, 435, 917
CpoI CGGWCCG 1 cut(s) 435
CseI GACGC 1 cut(s) 1109
Csp6I GTAC 2 cut(s) 168, 961
CspI CGGWCCG 1 cut(s) 435
CviAII CATG 7 cut(s) 85, 278, 722, 944, 983, 1003, 1128
CviQI GTAC 2 cut(s) 168, 961
DdeI CTNAG 3 cut(s) 548, 567, 798
DpnI GATC 2 cut(s) 39, 1203
DpnII GATC 2 cut(s) 37, 1201
Eco130I CCWWGG 3 cut(s) 66, 98, 809
Eco147I AGGCCT 1 cut(s) 97
Eco47I GGWCC 3 cut(s) 313, 435, 917
Eco72I CACGTG 1 cut(s) 578
Eco88I CYCGRG 1 cut(s) 948
EcoNI CCTNNNNNAGG 1 cut(s) 1069
EcoRI GAATTC 1 cut(s) 1196
EcoRII CCWGG 2 cut(s) 92, 538
EcoT14I CCWWGG 3 cut(s) 66, 98, 809
ErhI CCWWGG 3 cut(s) 66, 98, 809
FaeI CATG 7 cut(s) 88, 281, 725, 947, 986, 1006, 1131
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FatI CATG 7 cut(s) 84, 277, 721, 943, 982, 1002, 1127
Fnu4HI GCNGC 5 cut(s) 308, 417, 534, 1080, 1083
FokI GGATG 6 cut(s) 506, 573, 826, 889, 1054, 1133
Fsp4HI GCNGC 5 cut(s) 308, 417, 534, 1080, 1083
FspBI CTAG 2 cut(s) 854, 1208
GlaI GCGC 2 cut(s) 54, 56
GluI GCNGC 5 cut(s) 308, 417, 534, 1080, 1083
GsaI CCCAGC 1 cut(s) 1154
GsuI CTGGAG 1 cut(s) 696
HaeIII GGCC 1 cut(s) 97
HapII CCGG 2 cut(s) 311, 429
HgaI GACGC 1 cut(s) 1109
HhaI GCGC 2 cut(s) 55, 57
Hin1II CATG 7 cut(s) 88, 281, 725, 947, 986, 1006, 1131
Hin6I GCGC 2 cut(s) 53, 55
HinP1I GCGC 2 cut(s) 53, 55
HindIII AAGCTT 1 cut(s) 73
HinfI GANTC 1 cut(s) 1029
HpaII CCGG 2 cut(s) 311, 429
HphI GGTGA 1 cut(s) 844
Hpy166II GTNNAC 2 cut(s) 835, 920
Hpy188I TCNGA 4 cut(s) 127, 144, 736, 979
Hpy188III TCNNGA 5 cut(s) 259, 506, 550, 906, 1222
Hpy8I GTNNAC 2 cut(s) 835, 920
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 4 cut(s) 404, 851, 990, 1219
HpyCH4III ACNGT 4 cut(s) 325, 424, 556, 972
HpyCH4IV ACGT 4 cut(s) 129, 254, 577, 1135
HpyF10VI GCNNNNNNNGC 4 cut(s) 307, 425, 1088, 1151
HpyF3I CTNAG 3 cut(s) 548, 567, 798
HpySE526I ACGT 4 cut(s) 129, 254, 577, 1135
Hsp92II CATG 7 cut(s) 88, 281, 725, 947, 986, 1006, 1131
HspAI GCGC 2 cut(s) 53, 55
Kzo9I GATC 2 cut(s) 37, 1201
LmnI GCTCC 2 cut(s) 1150, 1159
Lsp1109I GCAGC 4 cut(s) 319, 403, 545, 1069
LweI GCATC 4 cut(s) 372, 508, 911, 1111
MaeI CTAG 2 cut(s) 854, 1208
MaeII ACGT 4 cut(s) 129, 254, 577, 1135
MaeIII GTNAC 2 cut(s) 448, 562
MalI GATC 2 cut(s) 39, 1203
MboI GATC 2 cut(s) 37, 1201
MboII GAAGA 3 cut(s) 814, 931, 1205
MluCI AATT 8 cut(s) 192, 217, 356, 592, 692, 699, 1068, 1196
MmeI TCCRAC 2 cut(s) 150, 739
MseI TTAA 5 cut(s) 459, 636, 702, 767, 1169
MspA1I CMGCKG 1 cut(s) 1082
MspI CCGG 2 cut(s) 311, 429
MspR9I CCNGG 2 cut(s) 94, 540
MvaI CCWGG 2 cut(s) 94, 540
MvnI CGCG 2 cut(s) 55, 891
MwoI GCNNNNNNNGC 4 cut(s) 307, 425, 1088, 1151
NdeII GATC 2 cut(s) 37, 1201
NlaIII CATG 7 cut(s) 88, 281, 725, 947, 986, 1006, 1131
NlaIV GGNNCC 2 cut(s) 286, 918
NmuCI GTSAC 1 cut(s) 448
NspI RCATGY 1 cut(s) 1131
PaeR7I CTCGAG 1 cut(s) 948
PaqCI CACCTGC 1 cut(s) 1076
PauI GCGCGC 1 cut(s) 53
PceI AGGCCT 1 cut(s) 97
PfeI GAWTC 1 cut(s) 1029
PflMI CCANNNNNTGG 2 cut(s) 99, 1163
PkrI GCNGC 5 cut(s) 309, 418, 535, 1081, 1084
PmaCI CACGTG 1 cut(s) 578
PmlI CACGTG 1 cut(s) 578
Ppu21I YACGTR 1 cut(s) 578
Psp6I CCWGG 2 cut(s) 92, 538
PspCI CACGTG 1 cut(s) 578
PspFI CCCAGC 1 cut(s) 1150
PspGI CCWGG 2 cut(s) 92, 538
PspN4I GGNNCC 2 cut(s) 286, 918
PspPI GGNCC 3 cut(s) 313, 435, 917
PspXI VCTCGAGB 1 cut(s) 948
PstI CTGCAG 1 cut(s) 1087
PstNI CAGNNNCTG 2 cut(s) 385, 566
PteI GCGCGC 1 cut(s) 53
RsaI GTAC 2 cut(s) 169, 962
RsaNI GTAC 2 cut(s) 168, 961
Rsr2I CGGWCCG 1 cut(s) 435
RsrII CGGWCCG 1 cut(s) 435
SaqAI TTAA 5 cut(s) 459, 636, 702, 767, 1169
SatI GCNGC 5 cut(s) 308, 417, 534, 1080, 1083
Sau3AI GATC 2 cut(s) 37, 1201
Sau96I GGNCC 3 cut(s) 313, 435, 917
ScrFI CCNGG 2 cut(s) 94, 540
SfaNI GCATC 4 cut(s) 372, 508, 911, 1111
SfcI CTRYAG 1 cut(s) 1083
Sfr274I CTCGAG 1 cut(s) 948
SinI GGWCC 3 cut(s) 313, 435, 917
SlaI CTCGAG 1 cut(s) 948
SmlI CTYRAG 2 cut(s) 504, 948
SmoI CTYRAG 2 cut(s) 504, 948
Sse9I AATT 8 cut(s) 192, 217, 356, 592, 692, 699, 1068, 1196
SseBI AGGCCT 1 cut(s) 97
SsiI CCGC 2 cut(s) 1080, 1205
SspI AATATT 1 cut(s) 529
SspMI CTAG 2 cut(s) 854, 1208
StuI AGGCCT 1 cut(s) 97
StyD4I CCNGG 2 cut(s) 92, 538
StyI CCWWGG 3 cut(s) 66, 98, 809
TaaI ACNGT 4 cut(s) 325, 424, 556, 972
TaiI ACGT 4 cut(s) 132, 257, 580, 1138
TaqI TCGA 4 cut(s) 114, 949, 1200, 1223
TaqII GACCGA 1 cut(s) 452
TasI AATT 8 cut(s) 192, 217, 356, 592, 692, 699, 1068, 1196
TatI WGTACW 1 cut(s) 960
TauI GCSGC 1 cut(s) 1082
TfiI GAWTC 1 cut(s) 1029
Tru1I TTAA 5 cut(s) 459, 636, 702, 767, 1169
Tru9I TTAA 5 cut(s) 459, 636, 702, 767, 1169
TscAI CASTG 2 cut(s) 328, 781
TseFI GTSAC 1 cut(s) 448
TseI GCWGC 4 cut(s) 307, 416, 533, 1082
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 4 cut(s) 73, 738, 999, 1206
TspGWI ACGGA 1 cut(s) 448
TspRI CASTG 2 cut(s) 328, 781
Van91I CCANNNNNTGG 2 cut(s) 99, 1163
VpaK11BI GGWCC 3 cut(s) 313, 435, 917
XagI CCTNNNNNAGG 1 cut(s) 1069
XapI RAATTY 5 cut(s) 192, 217, 356, 592, 1196
XceI RCATGY 1 cut(s) 1131
XhoI CTCGAG 1 cut(s) 948
XspI CTAG 2 cut(s) 854, 1208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.