RLG00000010026

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0007443
Physical Location & Seq
Forward (+)
61877736 .. 61881367
3632 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010026

Sequence Viewer

Length: 1986 bp
ATGGCGTCGTCTTATCTCGGCGCCTGTACTCCACGCGCCTCCCAGTTCTCCTTACACAGGCACAGACACCGATGCCCGATCCTTCTCGAGCAGGCGCGAACCGCGCCGTTCGCCGGAAACAGAGGAGTTAGCTTGAAGGTCAGGGCGGCGTCTTCGGATGGCGGCGGCGGGGACTCGTACCTCGGAATGTGGAAGAAGGCGATGGAGAACGAGAAGAAAGCCGTCAAGTTCCAGAAAATCGTCGAGAATTCAGCTCCGGCGGACAACGACGTCGCCGGCGCCGGCGAGAGCATTGTGGAGAATCTGGAGCATAAGAGCCAGGAGTTCGAGAAGATTCTGGAGGTTCCGAAAGAAGAGAGGGATCGGATTCAGCGAATGCAGGTCGTCGATAGGGCGGCGGCGGCGATCGCGGCGGCTCGCGCGCTTTTGAAAGAGAGCGGCTTGAAGGAGTCCGGTTCAGGTGAGCCGGGTTCGGGTGGAGTGAGGACTGAAGCGGATTTGGGTGGAGTGAGGACTGAGCCGGATTTGAGTGGAGCAATTGAGGCTCCTGATGAAGGAATGCAGGATGGGAGTATATCTGTGCCTCAGTCAGAAGAAAGTTCAGGAACTTGGACTCCGGGTCCAGACTTTTGGTCCTGGACCCCGCCGAGTGGTGATAGTTCAGATGATATAATTGACTTGCAGGCAGCGAAAAAGCCTTCAGCAAATCCAAACTTGAGTTTTCCAGTGAAGGAGAAAGAGCGATCCTTGGATGTTCTCTCAATTCCCCTTCAGAGTAATCTGTCTCAAATTCCTCTTCCCCCTTTGCAATCACTGAAGGAGGTCGAGAAAGTGGATGTCGCTGAGTCCACGTCCACAAAAGAGGAACATGAACTTGGACATGAGTTTTCAGTACATGCAGCAGAAGCTGCTCGTGCTCTTGATAAGGCGGATCAGGAGTCATTATCTGGTGTGTATACGGATGGAGCTAGGTGGTGGAAGGTATCTGGAGTTGAGCAAAGGCCTGATGGCGTGACGTGCAGGTGGACAATGATCAGAGGTGTCAGTGCCGACCAAGTTACTGAGTGGCAAGATAAGTACTGGGAGGCTGCCGATGAGTTAGGCCACAAGGAGCTTGGTTCAGAAAAATCGGGACGTGATGCATACGGAAATGTTTGGCGTGAACATTGGAAAGAAGCTATGTGGCAGAATTCTGGGCTTGTACATATGGAGAAATCTGCAGACAAGTGGGGAAAGAATGGTAGAGGTGATGAATGGCAAGAGAAATGGTGGGAGCATTATGATGCTTCAGGCCAAGCAGAGAAATGGGCTCATAAGTGGTGTACAATTGATCCAAACACACCAGTTGATGTTGGTCATGCTCACATTTGGCATGAAAGGTGGGGTGAAAAGTACGATGGACATGGTGGGAGTAATAAATACACTGACAAATGGGCTGAACGATGCGAGGGAGATGGTTGGTCAAAATGGGGTGACAAATGGGATGAACATTTTGATCCAAATGGCCATGGTGTGAAGCAGGGTGAAACATGGTGGGAAGGCAAGTATGGAGAGCGGTGGAACAAAACATGGGGCGAGGGCCACAATGGTTCAGGGTGGATTCACAAGTATGGGAAGAGCAGCAGCGGCGATCACTGGGATACACATCAGCAGGAAGACACCTGGTATGAGAGATTCCCTCACTTCGGTTTCTATCACTGCTTTGAAAACTCAGTTCAGCTCAGGGAAGTTCGAAAGCCATCAGAGATTGCATCGCAGATGGCTTCAGAGACTTCATCCGAGATGCCTTCTGAGACTTCGTCGGAGATGCCTTCATCAGAGATGCTTTCAGAGACTTCATCAGAGATGCCTCCAGAGACTTCATCTGAGATACCTTCAGAGACTTCATCGGAGATACCTTCAGAAATTCCATCGGACATGTCATCAGACGGTCCATTGGAGTTGCCCTCAGAGCTTCTATTGAAGAGACAATCAGAGGTGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

662

Amino Acids

73.7

Weight (kDa)

5.0

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1011
AasI GACNNNNNNGTC 1 cut(s) 269
AatII GACGTC 1 cut(s) 273
Acc36I ACCTGC 2 cut(s) 370, 1011
AccB1I GGYRCC 2 cut(s) 20, 278
AccBSI CCGCTC 2 cut(s) 438, 1555
AccI GTMKAC 1 cut(s) 956
AccII CGCG 6 cut(s) 36, 97, 104, 410, 420, 422
AclWI GGATC 6 cut(s) 73, 369, 738, 939, 1325, 1490
AcoI YGGCCR 1 cut(s) 1504
AcsI RAATTY 4 cut(s) 247, 789, 1189, 1905
AcuI CTGAAG 8 cut(s) 510, 684, 755, 836, 1272, 1749, 1860, 1884
AcyI GRCGYC 5 cut(s) 5, 21, 149, 270, 279
AfaI GTAC 7 cut(s) 28, 179, 894, 1079, 1203, 1324, 1394
AfiI CCNNNNNNNGG 6 cut(s) 57, 113, 473, 554, 650, 1685
AflIII ACRYGT 1 cut(s) 1917
AgsI TTSAA 5 cut(s) 136, 430, 445, 1706, 1963
AhdI GACNNNNNGTC 2 cut(s) 618, 631
AjiI CACGTC 3 cut(s) 852, 1017, 1136
AjnI CCWGG 3 cut(s) 318, 635, 1661
AjuI GAANNNNNNNTTGG 2 cut(s) 858, 890
AloI GAACNNNNNNTCC 2 cut(s) 598, 630
AluBI AGCT 8 cut(s) 132, 254, 908, 968, 1114, 1178, 1720, 1954
AluI AGCT 8 cut(s) 132, 254, 908, 968, 1114, 1178, 1720, 1954
Alw21I GWGCWC 1 cut(s) 919
Alw26I GTCTC 7 cut(s) 789, 1763, 1787, 1826, 1850, 1874, 1960
AlwI GGATC 6 cut(s) 73, 369, 738, 939, 1325, 1490
AlwNI CAGNNNCTG 1 cut(s) 908
Ama87I CYCGRG 1 cut(s) 86
AoxI GGCC 6 cut(s) 1001, 1102, 1291, 1504, 1579, 1980
ApeKI GCWGC 6 cut(s) 686, 899, 908, 1088, 1620, 1623
ApoI RAATTY 4 cut(s) 247, 789, 1189, 1905
AsiSI GCGATCGC 1 cut(s) 408
AspLEI GCGC 7 cut(s) 23, 38, 97, 106, 281, 422, 424
AspS9I GGNCC 5 cut(s) 620, 633, 639, 1579, 1931
AsuC2I CCSGG 2 cut(s) 468, 618
AsuHPI GGTGA 6 cut(s) 473, 665, 1259, 1397, 1484, 1535
AsuII TTCGAA 1 cut(s) 1732
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 4 cut(s) 620, 633, 639, 1931
BalI TGGCCA 1 cut(s) 1506
BanI GGYRCC 2 cut(s) 20, 278
BanII GRGCYC 1 cut(s) 1312
BauI CACGAG 1 cut(s) 912
BbsI GAAGAC 2 cut(s) 144, 1662
Bbv12I GWGCWC 1 cut(s) 919
BbvI GCAGC 6 cut(s) 698, 895, 911, 1075, 1632, 1635
BceAI ACGGC 2 cut(s) 91, 206
BciT130I CCWGG 3 cut(s) 320, 637, 1663
BciVI GTATCC 1 cut(s) 1633
BclI TGATCA 1 cut(s) 1032
BcnI CCSGG 2 cut(s) 468, 618
BcoDI GTCTC 7 cut(s) 789, 1763, 1787, 1826, 1850, 1874, 1960
BfaI CTAG 1 cut(s) 969
BfmI CTRYAG 1 cut(s) 1218
BfoI RGCGCY 2 cut(s) 24, 282
BfuAI ACCTGC 2 cut(s) 370, 1011
BfuI GTATCC 1 cut(s) 1633
BmcAI AGTACT 1 cut(s) 1079
Bme1390I CCNGG 5 cut(s) 320, 468, 618, 637, 1663
Bme18I GGWCC 4 cut(s) 620, 633, 639, 1931
BmeRI GACNNNNNGTC 2 cut(s) 618, 631
BmeT110I CYCGRG 1 cut(s) 86
BmgBI CACGTC 3 cut(s) 852, 1017, 1136
BmgT120I GGNCC 5 cut(s) 620, 633, 639, 1579, 1931
BmiI GGNNCC 6 cut(s) 22, 280, 345, 546, 621, 641
BmrFI CCNGG 5 cut(s) 320, 468, 618, 637, 1663
BmrI ACTGGG 3 cut(s) 37, 1090, 1645
BmsI GCATC 9 cut(s) 62, 1129, 1273, 1433, 1760, 1773, 1797, 1812, 1836
BmuI ACTGGG 3 cut(s) 37, 1090, 1645
BpiI GAAGAC 2 cut(s) 144, 1662
BplI GAGNNNNNCTC 4 cut(s) 1663, 1695, 1931, 1963
BpmI CTGGAG 4 cut(s) 326, 359, 1008, 1836
Bpu10I CCTNAGC 1 cut(s) 1721
Bpu14I TTCGAA 1 cut(s) 1732
BpuEI CTTGAG 1 cut(s) 736
BpuMI CCSGG 2 cut(s) 468, 618
BsaBI GATNNNNATC 1 cut(s) 1644
BsaHI GRCGYC 5 cut(s) 5, 21, 149, 270, 279
BsaJI CCNNGG 3 cut(s) 181, 747, 1507
BsaWI WCCGGW 1 cut(s) 452
BsaXI ACNNNNNCTCC 2 cut(s) 598, 628
Bsc4I CCNNNNNNNGG 6 cut(s) 57, 113, 473, 554, 650, 1685
Bse118I RCCGGY 2 cut(s) 275, 281
Bse1I ACTGG 5 cut(s) 43, 725, 1085, 1343, 1640
Bse8I GATNNNNATC 1 cut(s) 1644
BseBI CCWGG 3 cut(s) 320, 637, 1663
BseDI CCNNGG 3 cut(s) 181, 747, 1507
BseGI GGATG 7 cut(s) 163, 571, 757, 841, 967, 1489, 1775
BseJI GATNNNNATC 1 cut(s) 1644
BseLI CCNNNNNNNGG 6 cut(s) 57, 113, 473, 554, 650, 1685
BseMII CTCAG 9 cut(s) 507, 599, 834, 1053, 1725, 1735, 1782, 1857, 1962
BseNI ACTGG 5 cut(s) 43, 725, 1085, 1343, 1640
BsePI GCGCGC 1 cut(s) 420
BseRI GAGGAG 1 cut(s) 138
BseXI GCAGC 6 cut(s) 698, 895, 911, 1075, 1632, 1635
BsgI GTGCAG 1 cut(s) 1039
Bsh1236I CGCG 6 cut(s) 36, 97, 104, 410, 420, 422
Bsh1285I CGRYCG 1 cut(s) 408
BshFI GGCC 6 cut(s) 1003, 1104, 1293, 1506, 1581, 1982
BshNI GGYRCC 2 cut(s) 20, 278
BsiEI CGRYCG 1 cut(s) 408
BsiHKAI GWGCWC 1 cut(s) 919
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 8 cut(s) 114, 257, 276, 282, 453, 467, 521, 617
BslFI GGGAC 2 cut(s) 185, 1146
BslI CCNNNNNNNGG 6 cut(s) 57, 113, 473, 554, 650, 1685
BsmAI GTCTC 7 cut(s) 789, 1763, 1787, 1826, 1850, 1874, 1960
BsmFI GGGAC 2 cut(s) 185, 1146
BsmI GAATGC 2 cut(s) 381, 564
BsnI GGCC 6 cut(s) 1003, 1104, 1293, 1506, 1581, 1982
BsoBI CYCGRG 1 cut(s) 86
Bsp119I TTCGAA 1 cut(s) 1732
Bsp1286I GDGCHC 2 cut(s) 919, 1312
Bsp1407I TGTACA 2 cut(s) 1201, 1322
Bsp143I GATC 9 cut(s) 78, 361, 405, 743, 931, 1032, 1330, 1495, 1630
Bsp19I CCATGG 1 cut(s) 1507
BspANI GGCC 6 cut(s) 1003, 1104, 1293, 1506, 1581, 1982
BspCNI CTCAG 9 cut(s) 508, 598, 835, 1054, 1724, 1734, 1783, 1858, 1961
BspFNI CGCG 6 cut(s) 36, 97, 104, 410, 420, 422
BspLI GGNNCC 6 cut(s) 22, 280, 345, 546, 621, 641
BspMAI CTGCAG 1 cut(s) 1222
BspMI ACCTGC 2 cut(s) 370, 1011
BspPI GGATC 6 cut(s) 73, 369, 738, 939, 1325, 1490
BspQI GCTCTTC 1 cut(s) 1610
BspT104I TTCGAA 1 cut(s) 1732
BspT107I GGYRCC 2 cut(s) 20, 278
BsrBI CCGCTC 2 cut(s) 438, 1555
BsrFI RCCGGY 2 cut(s) 275, 281
BsrGI TGTACA 2 cut(s) 1201, 1322
BsrI ACTGG 5 cut(s) 43, 725, 1085, 1343, 1640
BssAI RCCGGY 2 cut(s) 275, 281
BssECI CCNNGG 3 cut(s) 181, 747, 1507
BssHII GCGCGC 1 cut(s) 420
BssMI GATC 9 cut(s) 78, 361, 405, 743, 931, 1032, 1330, 1495, 1630
BssNAI GTATAC 1 cut(s) 957
BssNI GRCGYC 5 cut(s) 5, 21, 149, 270, 279
BssSI CACGAG 1 cut(s) 912
BssT1I CCWWGG 2 cut(s) 747, 1507
Bst1107I GTATAC 1 cut(s) 957
Bst2BI CACGAG 1 cut(s) 912
Bst2UI CCWGG 3 cut(s) 320, 637, 1663
Bst4CI ACNGT 1 cut(s) 1931
Bst6I CTCTTC 4 cut(s) 348, 801, 1610, 1958
BstACI GRCGYC 5 cut(s) 5, 21, 149, 270, 279
BstAPI GCANNNNNTGC 1 cut(s) 908
BstAUI TGTACA 2 cut(s) 1201, 1322
BstBI TTCGAA 1 cut(s) 1732
BstC8I GCNNGC 6 cut(s) 93, 277, 283, 418, 422, 684
BstDEI CTNAG 9 cut(s) 516, 585, 843, 1062, 1711, 1721, 1791, 1866, 1948
BstDSI CCRYGG 1 cut(s) 1507
BstENI CCTNNNNNAGG 2 cut(s) 55, 552
BstF5I GGATG 7 cut(s) 163, 571, 757, 841, 967, 1489, 1775
BstFNI CGCG 6 cut(s) 36, 97, 104, 410, 420, 422
BstH2I RGCGCY 2 cut(s) 24, 282
BstHHI GCGC 7 cut(s) 23, 38, 97, 106, 281, 422, 424
BstKTI GATC 9 cut(s) 81, 364, 408, 746, 934, 1035, 1333, 1498, 1633
BstMAI GTCTC 7 cut(s) 789, 1763, 1787, 1826, 1850, 1874, 1960
BstMBI GATC 9 cut(s) 78, 361, 405, 743, 931, 1032, 1330, 1495, 1630
BstMCI CGRYCG 1 cut(s) 408
BstNI CCWGG 3 cut(s) 320, 637, 1663
BstNSI RCATGY 2 cut(s) 899, 1921
BstSCI CCNGG 5 cut(s) 318, 466, 616, 635, 1661
BstSFI CTRYAG 1 cut(s) 1218
BstUI CGCG 6 cut(s) 36, 97, 104, 410, 420, 422
BstV1I GCAGC 6 cut(s) 698, 895, 911, 1075, 1632, 1635
BstV2I GAAGAC 2 cut(s) 144, 1662
BstXI CCANNNNNNTGG 1 cut(s) 630
BstZ17I GTATAC 1 cut(s) 957
BsuI GTATCC 1 cut(s) 1633
BsuRI GGCC 6 cut(s) 1003, 1104, 1293, 1506, 1581, 1982
BtgI CCRYGG 1 cut(s) 1507
BtgZI GCGATG 2 cut(s) 215, 1737
BtrI CACGTC 3 cut(s) 852, 1017, 1136
BtsCI GGATG 7 cut(s) 163, 571, 757, 841, 967, 1489, 1775
BtsI GCAGTG 1 cut(s) 1696
BtsIMutI CAGTG 6 cut(s) 732, 812, 1051, 1422, 1633, 1696
BveI ACCTGC 2 cut(s) 370, 1011
Cac8I GCNNGC 6 cut(s) 93, 277, 283, 418, 422, 684
CaiI CAGNNNCTG 1 cut(s) 908
CfoI GCGC 7 cut(s) 23, 38, 97, 106, 281, 422, 424
Cfr10I RCCGGY 2 cut(s) 275, 281
Cfr13I GGNCC 5 cut(s) 620, 633, 639, 1579, 1931
CseI GACGC 1 cut(s) 138
CsiI ACCWGGT 1 cut(s) 1661
Csp6I GTAC 7 cut(s) 27, 178, 893, 1078, 1202, 1323, 1393
CviQI GTAC 7 cut(s) 27, 178, 893, 1078, 1202, 1323, 1393
DdeI CTNAG 9 cut(s) 516, 585, 843, 1062, 1711, 1721, 1791, 1866, 1948
DinI GGCGCC 2 cut(s) 22, 280
DpnI GATC 9 cut(s) 80, 363, 407, 745, 933, 1034, 1332, 1497, 1632
DpnII GATC 9 cut(s) 78, 361, 405, 743, 931, 1032, 1330, 1495, 1630
DrdI GACNNNNNNGTC 1 cut(s) 269
DriI GACNNNNNGTC 2 cut(s) 618, 631
DseDI GACNNNNNNGTC 1 cut(s) 269
EaeI YGGCCR 1 cut(s) 1504
Eam1104I CTCTTC 4 cut(s) 348, 801, 1610, 1958
Eam1105I GACNNNNNGTC 2 cut(s) 618, 631
EarI CTCTTC 4 cut(s) 348, 801, 1610, 1958
EciI GGCGGA 2 cut(s) 275, 944
Eco130I CCWWGG 2 cut(s) 747, 1507
Eco147I AGGCCT 1 cut(s) 1003
Eco24I GRGCYC 1 cut(s) 1312
Eco47I GGWCC 4 cut(s) 620, 633, 639, 1931
Eco57I CTGAAG 8 cut(s) 510, 684, 755, 836, 1272, 1749, 1860, 1884
Eco88I CYCGRG 1 cut(s) 86
EcoNI CCTNNNNNAGG 2 cut(s) 55, 552
EcoRI GAATTC 2 cut(s) 247, 1189
EcoRII CCWGG 3 cut(s) 318, 635, 1661
EcoT14I CCWWGG 2 cut(s) 747, 1507
EcoT22I ATGCAT 1 cut(s) 1144
EcoT38I GRGCYC 1 cut(s) 1312
EgeI GGCGCC 2 cut(s) 22, 280
EheI GGCGCC 2 cut(s) 22, 280
ErhI CCWWGG 2 cut(s) 747, 1507
FaqI GGGAC 2 cut(s) 185, 1146
FauI CCCGC 2 cut(s) 161, 651
FauNDI CATATG 1 cut(s) 1206
FbaI TGATCA 1 cut(s) 1032
FblI GTMKAC 1 cut(s) 956
FokI GGATG 7 cut(s) 170, 578, 764, 848, 974, 1496, 1762
FriOI GRGCYC 1 cut(s) 1312
FspBI CTAG 1 cut(s) 969
GlaI GCGC 7 cut(s) 22, 37, 96, 105, 280, 421, 423
GsuI CTGGAG 4 cut(s) 326, 359, 1008, 1836
HaeII RGCGCY 2 cut(s) 24, 282
HaeIII GGCC 6 cut(s) 1003, 1104, 1293, 1506, 1581, 1982
HapII CCGG 8 cut(s) 114, 257, 276, 282, 453, 467, 521, 617
HgaI GACGC 1 cut(s) 138
HhaI GCGC 7 cut(s) 23, 38, 97, 106, 281, 422, 424
Hin1I GRCGYC 5 cut(s) 5, 21, 149, 270, 279
Hin6I GCGC 7 cut(s) 21, 36, 95, 104, 279, 420, 422
HinP1I GCGC 7 cut(s) 21, 36, 95, 104, 279, 420, 422
HpaII CCGG 8 cut(s) 114, 257, 276, 282, 453, 467, 521, 617
HphI GGTGA 6 cut(s) 473, 665, 1259, 1397, 1484, 1535
Hpy166II GTNNAC 6 cut(s) 849, 855, 957, 1026, 1163, 1323
Hpy8I GTNNAC 6 cut(s) 849, 855, 957, 1026, 1163, 1323
Hpy99I CGWCG 6 cut(s) 10, 245, 272, 275, 389, 1804
HpyCH4III ACNGT 1 cut(s) 1931
HpyCH4IV ACGT 4 cut(s) 270, 851, 1016, 1135
HpyCH4V TGCA 9 cut(s) 379, 562, 682, 808, 899, 1020, 1142, 1220, 1751
HpyF3I CTNAG 9 cut(s) 516, 585, 843, 1062, 1711, 1721, 1791, 1866, 1948
HpySE526I ACGT 4 cut(s) 270, 851, 1016, 1135
Hsp92I GRCGYC 5 cut(s) 5, 21, 149, 270, 279
HspAI GCGC 7 cut(s) 21, 36, 95, 104, 279, 420, 422
KasI GGCGCC 2 cut(s) 20, 278
KroI GCCGGC 2 cut(s) 275, 281
KroNI GCCGGC 2 cut(s) 277, 283
Ksp22I TGATCA 1 cut(s) 1032
Kzo9I GATC 9 cut(s) 78, 361, 405, 743, 931, 1032, 1330, 1495, 1630
LguI GCTCTTC 1 cut(s) 1610
LmnI GCTCC 7 cut(s) 259, 307, 533, 550, 965, 1111, 1273
Lsp1109I GCAGC 6 cut(s) 698, 895, 911, 1075, 1632, 1635
LweI GCATC 9 cut(s) 62, 1129, 1273, 1433, 1760, 1773, 1797, 1812, 1836
MabI ACCWGGT 1 cut(s) 1661
MaeI CTAG 1 cut(s) 969
MaeII ACGT 4 cut(s) 270, 851, 1016, 1135
MaeIII GTNAC 3 cut(s) 1012, 1057, 1472
MalI GATC 9 cut(s) 80, 363, 407, 745, 933, 1034, 1332, 1497, 1632
MbiI CCGCTC 2 cut(s) 438, 1555
MboI GATC 9 cut(s) 78, 361, 405, 743, 931, 1032, 1330, 1495, 1630
MfeI CAATTG 2 cut(s) 537, 1326
MhlI GDGCHC 2 cut(s) 919, 1312
MlsI TGGCCA 1 cut(s) 1506
MluCI AATT 8 cut(s) 247, 537, 672, 762, 789, 1189, 1326, 1905
MluNI TGGCCA 1 cut(s) 1506
Mly113I GGCGCC 2 cut(s) 21, 279
MlyI GAGTC 5 cut(s) 167, 458, 607, 854, 947
MmeI TCCRAC 1 cut(s) 1782
Mox20I TGGCCA 1 cut(s) 1506
Mph1103I ATGCAT 1 cut(s) 1144
MreI CGCCGGCG 2 cut(s) 275, 281
MroNI GCCGGC 2 cut(s) 275, 281
MscI TGGCCA 1 cut(s) 1506
MslI CAYNNNNRTG 2 cut(s) 1281, 1608
Msp20I TGGCCA 1 cut(s) 1506
MspA1I CMGCKG 1 cut(s) 1626
MspI CCGG 8 cut(s) 114, 257, 276, 282, 453, 467, 521, 617
MspR9I CCNGG 5 cut(s) 320, 468, 618, 637, 1663
MunI CAATTG 2 cut(s) 537, 1326
Mva1269I GAATGC 2 cut(s) 381, 564
MvaI CCWGG 3 cut(s) 320, 637, 1663
MvnI CGCG 6 cut(s) 36, 97, 104, 410, 420, 422
NaeI GCCGGC 2 cut(s) 277, 283
NarI GGCGCC 2 cut(s) 21, 279
NciI CCSGG 2 cut(s) 468, 618
NcoI CCATGG 1 cut(s) 1507
NdeI CATATG 1 cut(s) 1206
NdeII GATC 9 cut(s) 78, 361, 405, 743, 931, 1032, 1330, 1495, 1630
NgoMIV GCCGGC 2 cut(s) 275, 281
NlaIV GGNNCC 6 cut(s) 22, 280, 345, 546, 621, 641
NmeAIII GCCGAG 1 cut(s) 672
NmuCI GTSAC 2 cut(s) 1012, 1472
NsiI ATGCAT 1 cut(s) 1144
NspI RCATGY 2 cut(s) 899, 1921
NspV TTCGAA 1 cut(s) 1732
PaeR7I CTCGAG 1 cut(s) 86
PaqCI CACCTGC 1 cut(s) 1011
PauI GCGCGC 1 cut(s) 420
PceI AGGCCT 1 cut(s) 1003
PciI ACATGT 1 cut(s) 1917
PciSI GCTCTTC 1 cut(s) 1610
PcsI WCGNNNNNNNCGW 1 cut(s) 370
PctI GAATGC 2 cut(s) 381, 564
PdiI GCCGGC 2 cut(s) 277, 283
PfeI GAWTC 5 cut(s) 301, 334, 367, 1600, 1674
PflFI GACNNNGTC 1 cut(s) 1798
PfoI TCCNGGA 1 cut(s) 635
Ple19I CGATCG 1 cut(s) 408
PleI GAGTC 5 cut(s) 167, 457, 607, 853, 946
PluTI GGCGCC 2 cut(s) 24, 282
PpsI GAGTC 5 cut(s) 167, 457, 607, 853, 946
PscI ACATGT 1 cut(s) 1917
Psp6I CCWGG 3 cut(s) 318, 635, 1661
PspGI CCWGG 3 cut(s) 318, 635, 1661
PspN4I GGNNCC 6 cut(s) 22, 280, 345, 546, 621, 641
PspPI GGNCC 5 cut(s) 620, 633, 639, 1579, 1931
PstI CTGCAG 1 cut(s) 1222
PstNI CAGNNNCTG 1 cut(s) 908
PsyI GACNNNGTC 1 cut(s) 1798
PteI GCGCGC 1 cut(s) 420
PvuI CGATCG 1 cut(s) 408
RgaI GCGATCGC 1 cut(s) 408
RsaI GTAC 7 cut(s) 28, 179, 894, 1079, 1203, 1324, 1394
RsaNI GTAC 7 cut(s) 27, 178, 893, 1078, 1202, 1323, 1393
RseI CAYNNNNRTG 2 cut(s) 1281, 1608
SapI GCTCTTC 1 cut(s) 1610
Sau3AI GATC 9 cut(s) 78, 361, 405, 743, 931, 1032, 1330, 1495, 1630
Sau96I GGNCC 5 cut(s) 620, 633, 639, 1579, 1931
ScaI AGTACT 1 cut(s) 1079
SchI GAGTC 5 cut(s) 167, 458, 607, 854, 947
ScrFI CCNGG 5 cut(s) 320, 468, 618, 637, 1663
SduI GDGCHC 2 cut(s) 919, 1312
SexAI ACCWGGT 1 cut(s) 1661
SfaAI GCGATCGC 1 cut(s) 408
SfaNI GCATC 9 cut(s) 62, 1129, 1273, 1433, 1760, 1773, 1797, 1812, 1836
SfcI CTRYAG 1 cut(s) 1218
SfoI GGCGCC 2 cut(s) 22, 280
Sfr274I CTCGAG 1 cut(s) 86
SfuI TTCGAA 1 cut(s) 1732
SgfI GCGATCGC 1 cut(s) 408
SgrAI CRCCGGYG 2 cut(s) 275, 281
SinI GGWCC 4 cut(s) 620, 633, 639, 1931
SlaI CTCGAG 1 cut(s) 86
SmiMI CAYNNNNRTG 2 cut(s) 1281, 1608
SmlI CTYRAG 2 cut(s) 86, 715
SmoI CTYRAG 2 cut(s) 86, 715
Sse9I AATT 8 cut(s) 247, 537, 672, 762, 789, 1189, 1326, 1905
SseBI AGGCCT 1 cut(s) 1003
SspDI GGCGCC 2 cut(s) 20, 278
SspMI CTAG 1 cut(s) 969
StuI AGGCCT 1 cut(s) 1003
StyD4I CCNGG 5 cut(s) 318, 466, 616, 635, 1661
StyI CCWWGG 2 cut(s) 747, 1507
TaaI ACNGT 1 cut(s) 1931
TaiI ACGT 4 cut(s) 273, 854, 1019, 1138
TaqI TCGA 6 cut(s) 87, 243, 327, 387, 825, 1732
TasI AATT 8 cut(s) 247, 537, 672, 762, 789, 1189, 1326, 1905
TatI WGTACW 5 cut(s) 26, 892, 1077, 1201, 1322
TfiI GAWTC 5 cut(s) 301, 334, 367, 1600, 1674
TscAI CASTG 6 cut(s) 732, 819, 1051, 1429, 1640, 1703
TseFI GTSAC 2 cut(s) 1012, 1472
TseI GCWGC 6 cut(s) 686, 899, 908, 1088, 1620, 1623
Tsp45I GTSAC 2 cut(s) 1012, 1472
TspGWI ACGGA 2 cut(s) 974, 1161
TspRI CASTG 6 cut(s) 732, 819, 1051, 1429, 1640, 1703
Tth111I GACNNNGTC 1 cut(s) 1798
VpaK11BI GGWCC 4 cut(s) 620, 633, 639, 1931
XagI CCTNNNNNAGG 2 cut(s) 55, 552
XapI RAATTY 4 cut(s) 247, 789, 1189, 1905
XceI RCATGY 2 cut(s) 899, 1921
XcmI CCANNNNNNNNNTGG 1 cut(s) 1112
XhoI CTCGAG 1 cut(s) 86
XmiI GTMKAC 1 cut(s) 956
XspI CTAG 1 cut(s) 969
ZraI GACGTC 1 cut(s) 271
ZrmI AGTACT 1 cut(s) 1079
Zsp2I ATGCAT 1 cut(s) 1144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.