RLG00000010322

DDB1- and CUL4-associated factor homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013230
Physical Location & Seq
Reverse (-)
653782 .. 662363
8582 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010322

Sequence Viewer

Length: 5880 bp
ATGGAGGCCGCCATGGACGACGAGCTACAGACCCAGACCCAGGGCCAAGGCCAAGCCGCGCCACCAGCTCCGCCGCCTCCCCCGGCGCCGGAATCGCAGGCAGGCGAGGAGCCGGAGGAAGACGACGAAGACGAGGAGCCGAAGAATGAAGACGATGAAATGGTGGCCAAGGCTCAGAAGCTCATGGATAAGATTATTGCTTCGCCTAATAACCCCAGCCCCACTGTTCTTCACGCCCTCGCTTCACTTCTTGAAACGCAGGAGAAACGATACATGGAGGAGAGTGGTCATTCTTCCAGCAATGGTCGTGCTTCTCATAACATTGGACGGCTAGGGAACTTGGTTCGGGAACATGATGATTTCTTTGAATTAATATCTGCCAAGTATTTGTCAGATACGAGATACTCTGCGTCCGTCCAGGCAGCTGCTGCAAGGCTGTTTCTGAGCTGTTCACTGACTTTGATTTATCCTCATGTTTTTGAAGAAGACATTTTGGAAAAAATAAAGGACTGGGTGATGGATGAGACTTCTAGTGTCTCGGTCGAATACCAGAACTGGAAGCATGATTTGGGAGGAAAGGAGGTCTCAGATTTTGAAATGCTGAAGACATATTCTACTGGACTTCTTGCTGTTTGTTTGACTGGGGGTGGTAAAGTAGTGGAAGATGTGTTGACGTCCGGATTGTCAGCCAAACTTATGCGTTATCTTCGTGTACGTGTTTTGGGGGAGTCAAGTATTAGTCAGAAGGATTCTAGTCATTTAACAGAGACTAAAAACACCTCTGGTGTAAGAGGTAGAGATGAAGGTCGGGGTAGGGTTCGGCAGGTTCTGGAAACAACTCATTTTGATGATCCAAGGGTAACAAGTGAGAGGTGCTTGGATGACCAGCCTGTTGAAGGGAACCAGGACAGAGTTATCTGTAGGCAAGCATTTGGAGGAGAACGTTGGGTTGATGGTGGAGAACCACCTGATGGAATGGATGAAGGGGTTGAGATCAATGATGTTGATGGCAGTGATTCTCGTGATTTACGTGATGGGAAACAAAAATTTGGTGATTTTGATGAAAATGGGAGAGATGACTCCTCAAGGCGCAGACCTAACCGTGGATGGGCACGATCTAGAGGGAAGGGAAGGGCAAACGAAGGCGGTGTAGAAAATGAGCAGCTGTTGACCTCACCCGGATCTGCTATTCGGTTGGGACAGGGGCGAAGCTTTAGAGACAAGAGTACATCAAAGAATTCAGATGTGAAAAAAATATCAGATTCTAGGAAGTGTTTGGGCAGGAATGCCTCTGATGTTTTGTTTCTGGAAAGAGAGGATAATGATGAGTGCTTCCAGGATTGCACAGTTGGAAGTAAGGATATCTCTGATCTAGTAAAAAAAGCTGTTAGAGCTGCAGAAGCTGAAGCTAGAGCAGCCAATGCACCTGTAGAAGCCATCAAAGCTGCTGGTGATGCTGCTGCGGAAGTTGTCAAAAGTGCCGCTTTAGAGGAATTCACAAGTACAAACAACGAAGAAGCTGCAGTTTTGGCTGCTTCAAGAGCAGCATCTACTGTAGTTGATGCCCCCAATTCAATTGAAGCTTTAAGTGTGAATGCAGTAAATACCTCTGCTAGTGCTGAACCAATAACTTCAAGCTCCGAACCTCAAAACCATGAAGATGTTGAAGAATACTTTATTCCAAGTGTTGAATCCCTTGCACAGTTGAGAGAAAAATACTGTATTCAATGTCTTGAGAATCTTGGAGAATATGTTGAAGTTCTTGGACCTGTATTGCATGAGAAGGGAGTAGACGTATGTCTTGCCTTGTTGCAACGAAATTCAAGACATAAAGAACCGTCAAAGGTCGCAATGCTTTTGCCTGATGTAATGAATCTCATATGTGCTCTGGCCGCTCACCGGAAATTTGCTGCATTGTTCGTGGATAGGGGGGGCATGCAGAAACTGCTTGCTGTCCCTAGAGTTGCTCAAACCTACTTTGGCCTTTCTTCTTGCTTATTTACGATCGGTTCGCTGCAGGTTATTATGGAGCGTGTATGTGCTCTTCCCTCAGACCTGGTTTACCAGGTGGTTGAGTTGGCTCTCCATCTTCTCGAGTGCTCACAGGATCAAGCCAGGAAAAATGCAGCCTTATTTTTTTCTGCTGCATTTGTTTTCAAGGCAGTTCTTGATGCTTTTGATGCTCAGGATGGCTTACAGAAAATACTGGGCCTTTTAAATGATGCTGCATCAGTAAGATCTGGAGTAAATTCTGGGACATTAGGACTCTCGACCTCAGGCTCACTTCGAAATGATAGGTCATCGACAGAAGTTCTGACCTCATCAGAGAAACAGATCGCTTATCACACCTGTGTTGCTTTGCGTCAGTACTTTAGAGCTAATTTGATTTTGCTTGTGGATTCTCTTCGTCCAAATAAAAACAGTCGAAGTGCGGCTCGAAATCTTCCAAGTGTAAGGGCAGCTTACAAGCCACTTGACCTTAGCAATGAAGCTATTGATGCAGTATTTCTGCAGTTACAGAAGGACCGGAAACTCGGTCCTGCATTTGTAAGAACTCGTTGGCCTGCAGTTGATAGGTTTTTGGGATATAATGGACATATTACTATGTTGGAGTTATGCCAGGCCCCACCTGTTGAGCGCTATCTACATGATTTACTTCAATATGCATTGGGTGTTCTGCACATTGTTACATTGGTACCTAGCAGTCGCAAGATGATTGTGAATTCAACATTAAGCAACAATCGTGTTGGTATAGCAGTTATATTGGATGCAGCAAGTGTTAATGGTAGTTATGTGGACCCCGAGATCATTCAGCCAGCTCTAAATGTGTTAGTCAATCTCGTTTGTCCTCCACCTTCAATCAGCAACAAACCACCTTTACTTGCACAAAGTCAGCAGTCTGTTTCTGCCCCAACCTCAAATGCCCTTGCTATAGAGACTAGAGAGAAAAATACTGAACGTAATGTCTCAGATCGAGCTGGGGAATCAGCTGTTGTAGATCGAGGCAGTGTAGCAGCACTGGGTACTGGTACACAGTCAAACAGTAGCAATGCACTATCCTCTACCCCCACAGCAACTTCTGGATTGGTGGGGGATCGCAGGATATCTTTAGGAGTTGGGGCAGGTTGTGCTGGGCTTGCGGCACAACTGGAGCAAGGCTATCGGCAAGCAAGAGAGGCTGTTCGCTCCACCAACGGTATAAAGGTTCTGCTGCATCTTCTCCAACCACGCATATATTCACCTCCTGCCGCACTCGATTGTCTACGTGCGCTTGCCTGCCGAGTCCTTCTTGGTTTAGCGAGAGATGATACAATTGCACACATATTGACAAAGCTCCAGGTTGGAAAGAAACTATCAGAACTAATTCGAGATCAAACACAAGGAGCTGAGCAAGGCAGGTGGCAATCTGAACTTTCACAGGCAGCAATCGAGTTAATGGCGATTGTGACGAATTCTGGACGTGCTAGCACATTGGCTGCCACTGATGCCGCTATGCCAACTTTGAGGCGCATAGAACGGGCAGCTATAGCTGCTGCCACTCCTATCACTTACCATTCCAGGGAGCTTTTGGTTCTAATACATGAACACCTACAGGCATCTGGTTTGGCCACAACAGCTGCTTCACTGCTAAAAGAGGCCCAGTTGACGCCTTTGCCATCCTTGGCTGCCCCCTCATCTCTTGTTCATCAAGCTACACAAGAAGCCTCATCTCTGCAGCTTCAGTGGCCCTCTGGTCGCACCCCTATTGGATTTCTTACCAACAAATCAAAGATAGCAAGGGAGGAGGATTCAAGCTTGAAATGTGATTCTACTGTCTCATACTCAAAGAAAAAACCTTTGGTTTTCTCTCCAAGTTTTTGTCTTCAGTCAAGGAATCCGTTCCAGTCCCATGACTCTCACCCAACATTGGCCACAAATGTCTTAAGTGCATCGAAAGAGTTATCTGCACCTGCAAATGCATCAGAAGCTCCATCAGATATCCTGCCGAAACCTAGTATTGATACAGAGTACCAGTGTAAGACACCCATCCTGTTGCCGATGAAAAGAAAGTTATCCGAGTTAAAGGATCCCGGATGTTTGCCATCATCTGGGAAACGCATCCACACCGGGGACCAGGGATATCGGTCTCCAATTTTTCCCACACCCAATATTGTGCGGAAAAGCGGTCTACTAACTGATCTGGCTGGGTTCTCTACTCCTACTTCCAACATGAGAGATCAACATTGTCGATCCACACCTGCTTGTTTTTCATCAGAGTACCTGGATGATAACCAGTATGGTAATTCTAGTGTAGGTCTGGCAACACCATCCTCCCAACTTGGGCTTCAAGGCGACTGTCAACCAAGCAACCCAGAAAGATTGACTCTGGACTCTTTGGTTGTTCAGTATCTGAAGCACCAGCATCGCCAGTGTCCTGCCCCTATAACCACTCTTCCACCACTGTCCCTGTTACAGCCACATGTTTGTCCCGAACCAAGACGAACCCTTGATGCCCCTTCAAATGTAACTGCCCGGCTTGGCACCCGTGAGTTCAGAAGTATGTATGGTGGGGTGCATGGAAACCGCAGGGATCGTCAGTTTGTTTACAGTAGATTCCGGCCATGGCGGACTTGTAGGGATGAGACTGGTACCCCTTTGACATGCATCTCTTTTCTGAGTGACACTGCTCGTATTGCGGTAGGCAGTCATGGTGGAGAGCTCAAAATTTTTGATTCCAATAGCAGCAATGTATTGGAGAGTTGTCCAAGCCACCAGTCTCCTGTAACGCTTGTTCAGACATATCATTCAGGTGAAACCGAATTGGTGCTGTCTTCTAGTTCAGAAGATGTGAGGTTGTGGGATGCATCTACAGTCTCATCTGGGCCAATGTATTCATATGACGGGTGCAAGGCTGCAAGATTTAGCAATTCTGGGGATATTTTTGCAGCCCTATCATCAGAGCTGGCTCAAAAAGAAATTCTAATATATGACATCCAAACTAACCGGTTGGAATCAAAACTGTCAGATACAGCTGCTAGCACGGGACGTGGACATTCATATTCTCATATACACTTCAACCCTTCAGACACAATGCTGCTCTGGAATGGTGTCCTATGGGACAGGCGGGTTTCCAGCCCTGTTCATCGTTTTGATCAGTTTACGGATTATGGGGGTGGTGGCTTCCATCCTGCTGGGAACGAGGTGATTATAAACTCTGAGGTCTGGGATCTCCGGAAGTTTAGACTCCTCCGAAGTGTACCTTCATTAGACCAAACTACAATAACTTTTAATGCCCGTGGTGATGTAATTTATGCAATCCTCCGGAGAAATCTTGATGATGTAATGTCAGCGGTGAATACCCGTCGCGTAAAGCATCCTCTCTTTGCCGCTTTTCGCACAGTGGATGCGGTTAACTACTCGGACATTGCCACGATTCCGGTTGATCGTTGTGTCCTCGACTTTGCAACGGAGCTAACTGATTCATTTCTTGGGCTGATCACAATGGATGACCAAGATGAGATGTTTGCTTCTGCTAGGGTTTATGAAATTGGTCGCCGAAAGCCGACAGATGATGATTCTGATCCTGATGATGCCGAGAGTGAAGAGGATGATGATGATGAAGAAGACGACGACGATGCGGATATGGACACTCTACTGGGCCCTGATCTTGACGGGGATGGTGATAGTGATATGAGTAATGATGATGATGATGATAGTGGGAGCGACCTTGATGACGACGATGAGGGAGATTATATGATGGATGGGGACTTTGATGGAGGAGGTGGAATGCTTGAGATTTTGACAGACGGTGAGGAAGATGACGATGACAGTGATGAACTGGTTGAATCCTTTAGCAGTGGTGATGAGGAAGATTTTGCAGGCCATGGTTTCGGTTATTAA

Protein Analysis

1960

Amino Acids

213.36

Weight (kDa)

4.95

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 1544 - 1619 9.9e-07 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 1544 - 1617 1.4e-06 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 1545 - 1669 9.9e-08 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 1546 - 1692 1.7e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013230)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31160
fragaria_vesca FvH4_2g30560
malus_domestica MD08G1132100.v1.1 MD15G1113300.v1.1
prunus_persica Prupe.1G463700_v2.0.a1
pyrus_communis pycom08g11060 pycom15g10160
rosa_chinensis RchiOBHm_Chr6g0312521
rosa_laevigata RLG00000010322
rosa_multiflora Rmu_sc0003094.1_g000015 Rmu_sc0035272.1_g000001
rosa_roxburghii Rroxscaffold_7G00156780
rosa_rugosa Rorug06G0396000
rosa_samantha Rh6AG513400 Rh6BG526600 Rh6CG531500 Rh6DG515500
rosa_wichuraiana Rw6G044640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 5189
AarI CACCTGC 3 cut(s) 3387, 3958, 4246
AatII GACGTC 1 cut(s) 677
Acc36I ACCTGC 6 cut(s) 814, 2008, 3113, 3387, 3958, 4246
Acc65I GGTACC 2 cut(s) 2695, 4628
AccB1I GGYRCC 4 cut(s) 85, 2695, 4520, 4628
AccB7I CCANNNNNTGG 2 cut(s) 971, 4088
AccBSI CCGCTC 1 cut(s) 1895
AccI GTMKAC 3 cut(s) 1791, 3262, 4168
AccII CGCG 2 cut(s) 59, 5346
AccIII TCCGGA 3 cut(s) 677, 5211, 5301
AclI AACGTT 1 cut(s) 943
AcoI YGGCCR 5 cut(s) 165, 1890, 3606, 3909, 4598
AcuI CTGAAG 6 cut(s) 623, 1425, 3704, 3848, 4412, 5046
AcyI GRCGYC 3 cut(s) 86, 674, 3647
AfeI AGCGCT 1 cut(s) 2639
AflII CTTAAG 1 cut(s) 3922
AflIII ACRYGT 2 cut(s) 715, 4459
AgeI ACCGGT 1 cut(s) 4983
AjiI CACGTC 2 cut(s) 3461, 5027
AjuI GAANNNNNNNTTGG 6 cut(s) 551, 583, 3821, 3853, 4308, 4340
AleI CACNNNNGTG 1 cut(s) 2349
Alw21I GWGCWC 4 cut(s) 1888, 2044, 2102, 4701
AlwNI CAGNNNCTG 5 cut(s) 428, 829, 1403, 1945, 4390
Ama87I CYCGRG 2 cut(s) 2093, 2801
Aor13HI TCCGGA 3 cut(s) 677, 5211, 5301
Aor51HI AGCGCT 1 cut(s) 2639
ApaI GGGCCC 1 cut(s) 5642
AseI ATTAAT 1 cut(s) 371
AsiGI ACCGGT 1 cut(s) 4983
Asp700I GAANNNNTTC 2 cut(s) 3363, 3878
Asp718I GGTACC 2 cut(s) 2695, 4628
AspLEI GCGC 6 cut(s) 61, 88, 1092, 2640, 3271, 3510
AsuC2I CCSGG 5 cut(s) 83, 1179, 4071, 4108, 4513
AsuII TTCGAA 1 cut(s) 2287
AsuNHI GCTAGC 2 cut(s) 3464, 5015
AvaI CYCGRG 2 cut(s) 2093, 2801
AvaII GGWCC 5 cut(s) 1766, 2524, 2537, 2797, 4111
AxyI CCTNAGG 1 cut(s) 2275
BaeGI GKGCMC 2 cut(s) 1114, 5642
BaeI ACNNNNGTAYC 2 cut(s) 2679, 2712
BalI TGGCCA 3 cut(s) 167, 3608, 3911
BamHI GGATCC 1 cut(s) 4066
BanI GGYRCC 4 cut(s) 85, 2695, 4520, 4628
BanII GRGCYC 2 cut(s) 4701, 5642
BarI GAAGNNNNNNTAC 2 cut(s) 2028, 2060
BauI CACGAG 1 cut(s) 1020
BbsI GAAGAC 8 cut(s) 126, 135, 156, 492, 611, 3854, 4803, 5610
Bbv12I GWGCWC 4 cut(s) 1888, 2044, 2102, 4701
BceAI ACGGC 1 cut(s) 344
BcgI CGANNNNNNTGC 8 cut(s) 1502, 1536, 3143, 3177, 4385, 4419, 5597, 5631
BclI TGATCA 2 cut(s) 5131, 5475
BcnI CCSGG 5 cut(s) 83, 1179, 4071, 4108, 4513
BfoI RGCGCY 2 cut(s) 89, 2641
BfrI CTTAAG 1 cut(s) 3922
BfuAI ACCTGC 6 cut(s) 814, 2008, 3113, 3387, 3958, 4246
BglII AGATCT 1 cut(s) 2237
BlpI GCTNAGC 1 cut(s) 3387
BmcAI AGTACT 1 cut(s) 2369
Bme18I GGWCC 5 cut(s) 1766, 2524, 2537, 2797, 4111
BmeT110I CYCGRG 2 cut(s) 2093, 2801
BmgBI CACGTC 2 cut(s) 3461, 5027
BmrI ACTGGG 6 cut(s) 520, 651, 2216, 3029, 3634, 5645
BmtI GCTAGC 2 cut(s) 3468, 5019
BmuI ACTGGG 6 cut(s) 520, 651, 2216, 3029, 3634, 5645
BoxI GACNNNNGTC 1 cut(s) 1797
BpiI GAAGAC 8 cut(s) 126, 135, 156, 492, 611, 3854, 4803, 5610
BpmI CTGGAG 3 cut(s) 2262, 3170, 3320
Bpu10I CCTNAGC 2 cut(s) 2184, 2480
Bpu1102I GCTNAGC 1 cut(s) 3387
Bpu14I TTCGAA 1 cut(s) 2287
BpuEI CTTGAG 3 cut(s) 1069, 1754, 5792
BpuMI CCSGG 5 cut(s) 83, 1179, 4071, 4108, 4513
BsaAI YACGTR 3 cut(s) 716, 1031, 3266
BsaBI GATNNNNATC 1 cut(s) 5559
BsaHI GRCGYC 3 cut(s) 86, 674, 3647
BsaI GGTCTC 2 cut(s) 589, 4131
BsaWI WCCGGW 7 cut(s) 677, 1899, 2526, 4983, 5211, 5301, 5416
BsaXI ACNNNNNCTCC 4 cut(s) 1737, 1767, 1779, 1809
Bse118I RCCGGY 1 cut(s) 4983
Bse21I CCTNAGG 1 cut(s) 2275
Bse3DI GCAATG 6 cut(s) 307, 1857, 2491, 3055, 4732, 5403
Bse8I GATNNNNATC 1 cut(s) 5559
BseAI TCCGGA 3 cut(s) 677, 5211, 5301
BseJI GATNNNNATC 1 cut(s) 5559
BseMI GCAATG 6 cut(s) 307, 1857, 2491, 3055, 4732, 5403
BseRI GAGGAG 8 cut(s) 122, 149, 293, 951, 1072, 3797, 5216, 5772
BseSI GKGCMC 2 cut(s) 1114, 5642
BseYI CCCAGC 5 cut(s) 215, 2978, 3131, 4184, 5171
BsgI GTGCAG 2 cut(s) 2663, 3930
Bsh1236I CGCG 2 cut(s) 59, 5346
Bsh1285I CGRYCG 2 cut(s) 543, 2007
BshNI GGYRCC 4 cut(s) 85, 2695, 4520, 4628
BshTI ACCGGT 1 cut(s) 4983
BsiEI CGRYCG 2 cut(s) 543, 2007
BsiHKAI GWGCWC 4 cut(s) 1888, 2044, 2102, 4701
BsiHKCI CYCGRG 2 cut(s) 2093, 2801
BsmI GAATGC 3 cut(s) 1291, 1600, 5772
Bso31I GGTCTC 2 cut(s) 589, 4131
BsoBI CYCGRG 2 cut(s) 2093, 2801
Bsp119I TTCGAA 1 cut(s) 2287
Bsp120I GGGCCC 1 cut(s) 5638
Bsp1286I GDGCHC 6 cut(s) 1114, 1888, 2044, 2102, 4701, 5642
Bsp13I TCCGGA 3 cut(s) 677, 5211, 5301
Bsp1720I GCTNAGC 1 cut(s) 3387
Bsp19I CCATGG 3 cut(s) 12, 4601, 5863
BspEI TCCGGA 3 cut(s) 677, 5211, 5301
BspFNI CGCG 2 cut(s) 59, 5346
BspMAI CTGCAG 6 cut(s) 1399, 1525, 2019, 2514, 2569, 3717
BspMI ACCTGC 6 cut(s) 814, 2008, 3113, 3387, 3958, 4246
BspOI GCTAGC 2 cut(s) 3468, 5019
BspQI GCTCTTC 1 cut(s) 2049
BspT104I TTCGAA 1 cut(s) 2287
BspT107I GGYRCC 4 cut(s) 85, 2695, 4520, 4628
BspTI CTTAAG 1 cut(s) 3922
BspTNI GGTCTC 2 cut(s) 589, 4131
BsrBI CCGCTC 1 cut(s) 1895
BsrDI GCAATG 6 cut(s) 307, 1857, 2491, 3055, 4732, 5403
BsrFI RCCGGY 1 cut(s) 4983
BssAI RCCGGY 1 cut(s) 4983
BssNI GRCGYC 3 cut(s) 86, 674, 3647
BssSI CACGAG 1 cut(s) 1020
BssT1I CCWWGG 7 cut(s) 12, 46, 168, 854, 3660, 4601, 5863
Bst2BI CACGAG 1 cut(s) 1020
Bst6I CTCTTC 4 cut(s) 2049, 2409, 4437, 5577
BstACI GRCGYC 3 cut(s) 86, 674, 3647
BstAFI CTTAAG 1 cut(s) 3922
BstAPI GCANNNNNTGC 4 cut(s) 428, 1421, 1945, 3128
BstBAI YACGTR 3 cut(s) 716, 1031, 3266
BstBI TTCGAA 1 cut(s) 2287
BstDSI CCRYGG 5 cut(s) 12, 1102, 4601, 5275, 5863
BstENI CCTNNNNNAGG 1 cut(s) 894
BstFNI CGCG 2 cut(s) 59, 5346
BstH2I RGCGCY 2 cut(s) 89, 2641
BstHHI GCGC 6 cut(s) 61, 88, 1092, 2640, 3271, 3510
BstMCI CGRYCG 2 cut(s) 543, 2007
BstNSI RCATGY 3 cut(s) 1939, 4463, 4644
BstPAI GACNNNNGTC 1 cut(s) 1797
BstSLI GKGCMC 2 cut(s) 1114, 5642
BstUI CGCG 2 cut(s) 59, 5346
BstV2I GAAGAC 8 cut(s) 126, 135, 156, 492, 611, 3854, 4803, 5610
BstX2I RGATCY 4 cut(s) 1181, 2237, 4066, 5206
BstXI CCANNNNNNTGG 1 cut(s) 5171
BstYI RGATCY 4 cut(s) 1181, 2237, 4066, 5206
Bsu36I CCTNAGG 1 cut(s) 2275
BtgI CCRYGG 5 cut(s) 12, 1102, 4601, 5275, 5863
BtgZI GCGATG 1 cut(s) 4388
BtrI CACGTC 2 cut(s) 3461, 5027
BtsI GCAGTG 5 cut(s) 1018, 3013, 3623, 4662, 5842
BveI ACCTGC 6 cut(s) 814, 2008, 3113, 3387, 3958, 4246
CaiI CAGNNNCTG 5 cut(s) 428, 829, 1403, 1945, 4390
CfoI GCGC 6 cut(s) 61, 88, 1092, 2640, 3271, 3510
Cfr10I RCCGGY 1 cut(s) 4983
CseI GACGC 3 cut(s) 399, 2351, 3655
CsiI ACCWGGT 2 cut(s) 2055, 2064
CspAI ACCGGT 1 cut(s) 4983
CspCI CAANNNNNGTGG 2 cut(s) 3380, 3415
DinI GGCGCC 1 cut(s) 87
DraI TTTAAA 1 cut(s) 2217
EaeI YGGCCR 5 cut(s) 165, 1890, 3606, 3909, 4598
Eam1104I CTCTTC 4 cut(s) 2049, 2409, 4437, 5577
EarI CTCTTC 4 cut(s) 2049, 2409, 4437, 5577
EciI GGCGGA 2 cut(s) 60, 4621
Ecl136II GAGCTC 1 cut(s) 4699
Eco130I CCWWGG 7 cut(s) 12, 46, 168, 854, 3660, 4601, 5863
Eco24I GRGCYC 2 cut(s) 4701, 5642
Eco31I GGTCTC 2 cut(s) 589, 4131
Eco32I GATATC 4 cut(s) 1363, 3105, 3979, 4121
Eco47I GGWCC 5 cut(s) 1766, 2524, 2537, 2797, 4111
Eco47III AGCGCT 1 cut(s) 2639
Eco53kI GAGCTC 1 cut(s) 4699
Eco57I CTGAAG 6 cut(s) 623, 1425, 3704, 3848, 4412, 5046
Eco81I CCTNAGG 1 cut(s) 2275
Eco88I CYCGRG 2 cut(s) 2093, 2801
EcoICRI GAGCTC 1 cut(s) 4699
EcoNI CCTNNNNNAGG 1 cut(s) 894
EcoO109I RGGNCCY 2 cut(s) 2623, 5639
EcoRI GAATTC 4 cut(s) 1237, 1493, 2722, 3451
EcoRV GATATC 4 cut(s) 1363, 3105, 3979, 4121
EcoT14I CCWWGG 7 cut(s) 12, 46, 168, 854, 3660, 4601, 5863
EcoT22I ATGCAT 4 cut(s) 2668, 3961, 4646, 4846
EcoT38I GRGCYC 2 cut(s) 4701, 5642
EgeI GGCGCC 1 cut(s) 87
EheI GGCGCC 1 cut(s) 87
ErhI CCWWGG 7 cut(s) 12, 46, 168, 854, 3660, 4601, 5863
FalI AAGNNNNNCTT 6 cut(s) 1468, 1500, 2446, 2478, 5224, 5256
FauI CCCGC 1 cut(s) 5097
FauNDI CATATG 2 cut(s) 1880, 4876
FbaI TGATCA 2 cut(s) 5131, 5475
FblI GTMKAC 3 cut(s) 1791, 3262, 4168
FriOI GRGCYC 2 cut(s) 4701, 5642
GlaI GCGC 6 cut(s) 60, 87, 1091, 2639, 3270, 3509
GsaI CCCAGC 5 cut(s) 219, 2982, 3135, 4188, 5175
GsuI CTGGAG 3 cut(s) 2262, 3170, 3320
HaeII RGCGCY 2 cut(s) 89, 2641
HgaI GACGC 3 cut(s) 399, 2351, 3655
HhaI GCGC 6 cut(s) 61, 88, 1092, 2640, 3271, 3510
Hin1I GRCGYC 3 cut(s) 86, 674, 3647
Hin6I GCGC 6 cut(s) 59, 86, 1090, 2638, 3269, 3508
HinP1I GCGC 6 cut(s) 59, 86, 1090, 2638, 3269, 3508
HincII GTYRAC 5 cut(s) 672, 1170, 3645, 4340, 5392
HindII GTYRAC 5 cut(s) 672, 1170, 3645, 4340, 5392
HindIII AAGCTT 3 cut(s) 1210, 1581, 3793
HpaI GTTAAC 1 cut(s) 5392
Hpy99I CGWCG 6 cut(s) 23, 128, 5346, 5612, 5615, 5720
HpyCH4IV ACGT 9 cut(s) 674, 715, 943, 1030, 1794, 2959, 3265, 3460, 5026
HpySE526I ACGT 9 cut(s) 674, 715, 943, 1030, 1794, 2959, 3265, 3460, 5026
Hsp92I GRCGYC 3 cut(s) 86, 674, 3647
HspAI GCGC 6 cut(s) 59, 86, 1090, 2638, 3269, 3508
KasI GGCGCC 1 cut(s) 85
Kpn2I TCCGGA 3 cut(s) 677, 5211, 5301
KpnI GGTACC 2 cut(s) 2699, 4632
Ksp22I TGATCA 2 cut(s) 5131, 5475
KspAI GTTAAC 1 cut(s) 5392
LguI GCTCTTC 1 cut(s) 2049
MabI ACCWGGT 2 cut(s) 2055, 2064
MaeII ACGT 9 cut(s) 674, 715, 943, 1030, 1794, 2959, 3265, 3460, 5026
MaeIII GTNAC 8 cut(s) 859, 2514, 2686, 3445, 4449, 4504, 4658, 4762
MbiI CCGCTC 1 cut(s) 1895
MfeI CAATTG 2 cut(s) 1575, 3312
MflI RGATCY 4 cut(s) 1181, 2237, 4066, 5206
MhlI GDGCHC 6 cut(s) 1114, 1888, 2044, 2102, 4701, 5642
MlsI TGGCCA 3 cut(s) 167, 3608, 3911
MluNI TGGCCA 3 cut(s) 167, 3608, 3911
Mly113I GGCGCC 1 cut(s) 86
MlyI GAGTC 8 cut(s) 737, 1073, 2259, 3291, 3887, 4357, 4364, 5217
MmeI TCCRAC 6 cut(s) 1330, 2589, 3247, 3322, 4230, 4968
Mox20I TGGCCA 3 cut(s) 167, 3608, 3911
Mph1103I ATGCAT 4 cut(s) 2668, 3961, 4646, 4846
MroI TCCGGA 3 cut(s) 677, 5211, 5301
MroXI GAANNNNTTC 2 cut(s) 3363, 3878
MscI TGGCCA 3 cut(s) 167, 3608, 3911
MslI CAYNNNNRTG 4 cut(s) 846, 1659, 2349, 4788
Msp20I TGGCCA 3 cut(s) 167, 3608, 3911
MspA1I CMGCKG 6 cut(s) 425, 1165, 2990, 3617, 5012, 5330
MspCI CTTAAG 1 cut(s) 3922
MunI CAATTG 2 cut(s) 1575, 3312
Mva1269I GAATGC 3 cut(s) 1291, 1600, 5772
MvnI CGCG 2 cut(s) 59, 5346
NarI GGCGCC 1 cut(s) 86
NciI CCSGG 5 cut(s) 83, 1179, 4071, 4108, 4513
NcoI CCATGG 3 cut(s) 12, 4601, 5863
NdeI CATATG 2 cut(s) 1880, 4876
NheI GCTAGC 2 cut(s) 3464, 5015
NmeAIII GCCGAG 2 cut(s) 3305, 5599
NmuCI GTSAC 2 cut(s) 3445, 4658
NsiI ATGCAT 4 cut(s) 2668, 3961, 4646, 4846
NspI RCATGY 3 cut(s) 1939, 4463, 4644
NspV TTCGAA 1 cut(s) 2287
OliI CACNNNNGTG 1 cut(s) 2349
PaeI GCATGC 1 cut(s) 1939
PaeR7I CTCGAG 1 cut(s) 2093
PaqCI CACCTGC 3 cut(s) 3387, 3958, 4246
PasI CCCWGGG 1 cut(s) 40
PciI ACATGT 1 cut(s) 4459
PciSI GCTCTTC 1 cut(s) 2049
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 1027
PctI GAATGC 3 cut(s) 1291, 1600, 5772
PdmI GAANNNNTTC 2 cut(s) 3363, 3878
PflMI CCANNNNNTGG 2 cut(s) 971, 4088
PfoI TCCNGGA 2 cut(s) 1335, 4069
PinAI ACCGGT 1 cut(s) 4983
Ple19I CGATCG 1 cut(s) 2007
PleI GAGTC 8 cut(s) 736, 1073, 2259, 3290, 3887, 4357, 4364, 5217
PluTI GGCGCC 1 cut(s) 89
PpsI GAGTC 8 cut(s) 736, 1073, 2259, 3290, 3887, 4357, 4364, 5217
Ppu21I YACGTR 3 cut(s) 716, 1031, 3266
PscI ACATGT 1 cut(s) 4459
PshAI GACNNNNGTC 1 cut(s) 1797
PshBI ATTAAT 1 cut(s) 371
PsiI TTATAA 1 cut(s) 5189
Psp124BI GAGCTC 1 cut(s) 4701
Psp1406I AACGTT 1 cut(s) 943
PspFI CCCAGC 5 cut(s) 215, 2978, 3131, 4184, 5171
PspOMI GGGCCC 1 cut(s) 5638
PsrI GAACNNNNNNTAC 2 cut(s) 4755, 4787
PstI CTGCAG 6 cut(s) 1399, 1525, 2019, 2514, 2569, 3717
PstNI CAGNNNCTG 5 cut(s) 428, 829, 1403, 1945, 4390
PsuI RGATCY 4 cut(s) 1181, 2237, 4066, 5206
PvuI CGATCG 1 cut(s) 2007
PvuII CAGCTG 5 cut(s) 425, 1165, 2990, 3617, 5012
RseI CAYNNNNRTG 4 cut(s) 846, 1659, 2349, 4788
SacI GAGCTC 1 cut(s) 4701
SapI GCTCTTC 1 cut(s) 2049
ScaI AGTACT 1 cut(s) 2369
SchI GAGTC 8 cut(s) 737, 1073, 2259, 3291, 3887, 4357, 4364, 5217
SduI GDGCHC 6 cut(s) 1114, 1888, 2044, 2102, 4701, 5642
SexAI ACCWGGT 2 cut(s) 2055, 2064
SfoI GGCGCC 1 cut(s) 87
Sfr274I CTCGAG 1 cut(s) 2093
SfuI TTCGAA 1 cut(s) 2287
SinI GGWCC 5 cut(s) 1766, 2524, 2537, 2797, 4111
SlaI CTCGAG 1 cut(s) 2093
SmiMI CAYNNNNRTG 4 cut(s) 846, 1659, 2349, 4788
SmlI CTYRAG 5 cut(s) 1084, 1733, 2093, 3922, 5771
SmoI CTYRAG 5 cut(s) 1084, 1733, 2093, 3922, 5771
SphI GCATGC 1 cut(s) 1939
SspDI GGCGCC 1 cut(s) 85
SspI AATATT 1 cut(s) 4150
SstI GAGCTC 1 cut(s) 4701
StyI CCWWGG 7 cut(s) 12, 46, 168, 854, 3660, 4601, 5863
TaiI ACGT 9 cut(s) 677, 718, 946, 1033, 1797, 2962, 3268, 3463, 5029
TaqII GACCGA 3 cut(s) 529, 2525, 4113
TatI WGTACW 3 cut(s) 1226, 1502, 2367
TseFI GTSAC 2 cut(s) 3445, 4658
Tsp45I GTSAC 2 cut(s) 3445, 4658
TspGWI ACGGA 4 cut(s) 403, 3867, 5156, 5462
Van91I CCANNNNNTGG 2 cut(s) 971, 4088
Vha464I CTTAAG 1 cut(s) 3922
VpaK11BI GGWCC 5 cut(s) 1766, 2524, 2537, 2797, 4111
VspI ATTAAT 1 cut(s) 371
XagI CCTNNNNNAGG 1 cut(s) 894
XbaI TCTAGA 1 cut(s) 1118
XceI RCATGY 3 cut(s) 1939, 4463, 4644
XcmI CCANNNNNNNNNTGG 1 cut(s) 3565
XhoI CTCGAG 1 cut(s) 2093
XmiI GTMKAC 3 cut(s) 1791, 3262, 4168
XmnI GAANNNNTTC 2 cut(s) 3363, 3878
ZraI GACGTC 1 cut(s) 675
ZrmI AGTACT 1 cut(s) 2369
Zsp2I ATGCAT 4 cut(s) 2668, 3961, 4646, 4846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.