Rh6AG513400

DDB1- and CUL4-associated factor homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0013230
Physical Location & Seq
Forward (+)
68377580 .. 68386881
9302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG513400.1

Sequence Viewer

Length: 5883 bp
ATGGAGGCCGCCATGGACGACGAGCTACAGACCCAGGGCCAGGGCCAAGGCCAAGCCGCGCCACCAGCTCCGCCGCCTCCCCCGGCGCCGGAATCGCAGGCAGGCGAGGAGCCGGAGGAAGACGACGAAGACGAGGAGCCGAAGAATGAAGACGATGAAATGGTGGCCAAGGCTCAGAAGCTCATGGATAAGATTATTGCTTCGCCTGATAACCCCAGCCCCACTGTTCTTCACGCCCTCGCTTCACTTCTGGAAACGCAGGAGAAACGATACATGGAGGAGAGTGGTCATTCTTCCAGCAATGGTCGTGCTTCTCATAACATTGGACGGCTAGGGAACTTGGTTCGGGAACATGATGACTTCTTTGAATTAATATCTGCCAAGTATTTGTCAGATACGAGATACTCTGCGTCCGTCCAGGCAGCTGCTGCAAGGCTGTTTCTGAGCTGTTCACTGACTTTGATTTATCCTCATGTTTTTGAAGAAGACGTTTTGGAGAAAATAAAGGACTGGGTGATGGATGAGACTTCCAGTATCTCGGTCGAATACCAGAACTGGAAGCATGATTTGGGAGGAAAGGAGGTCTCAGATTTTGAAATGCTGAAGACATATTCTACTGGACTTCTTGCTGTTTGTTTGACTGGGGATGGTCAAGTAGTGGAAGATGTGTTGACGTCCGGATTGTCAGCCAAACTTATGCGTTATCTTCGTGTCCGTGTTTTGGGGGAGTCAAGTATTAGTCAGAAGGATTCTAGTCATTTAACAGAGACTAAAAACACCTCTGGTGTAAGAGGTAGAGATGAAGGTCGGGGTAGGGTTCGGCAGGTTCTGGAAACAACTCATTTTGATGATCCAAGGGTAACAAGTGAGAGATGCTTGGAGGACCAGACTGTTGAAGGGAACCAGGACAGAAGTATCTGTAGGCAAGCGTTTGGAGGAGAACGTTGGGTTGATGGTGGAGAACCACCTGATGGAATGGATGAAGGGGTTGAGATCAATGATGTTGATGGCAGTGATTCTCGTGATTTACGTGATGGGAAACAAAAATTTGGTGATTTTGATGAAAATGGGAGAGATGACTCCTCAAGGCGCAGACCTAACCGTGGATGGGCACGATCTAGAGGGAAGGGAAGGGCAAACGAAGGTGGTGTAGAAAATGAGCAGCTGTTGACCTCACCCGGATCTGCTATTCGGTTGGGACAGGGGCGAAGCTTTAGAGACAAGAGTACATCAAAGAATTCAGACGTGAAAAAAATATCAGATTCTAGGAAGTGTTTGGGCAGGAATGCTTCTGATGTTTTGTTTCTGGAAAGAGAGGATAATGATGAGTGCTTCCAGGATTGCACAGTTGGAAGTAAGGATATCTCTGATCTAGTAAAAAAAGCAGTTCGAGCTGCAGAAGCTGAAGCTAGAGCAGCCAATGCACCTGTAGAAGCCATCAAAGCTGCTGGTGATGCTGCTGCGGAAGTTGTCAAATGTGCAGCTTTAGAGGAATTCACAACTACAAACAACGAAGAAGCTGCAGTTTTGGCTGCTTCAAGAGCAGCATCTACTGTAATTGATGCCGCCAATTCAATTGAAGCTCTAAGTGCGAATGCAGTAAATACCTCTGGTAGTGCTGAACCAATAACTTCAAGCTCCGAACCCCAAAACCATGAAGATGTTGAAGAATACTTTATTCCGAGTGTTGAATCCCTTGCACAGTTGAGAGAAAAATACTGTATTCAATGTCTTGAGTCTCTTGGAGAATATGTTGAAGTTCTTGGACCTGTATTGCATGAGAAGGGAGTAGACGTATGTCTTGCCTTGTTGCAACGAAATTCAAGACATAAAGAACCGTCGAAGGTCGCAATGCTTTTGCCTGATGTAATGAAACTCATATGTGCTCTGGCTGCTCACCGGAAATTTGCTGCATTGTTCGTGGATAGGGGTGGCATGCAGAAACTGCTTGCTGTCCCTAGAGTTGCTCAAACGTACTTTGGCCTTTCTTCTTGCTTATTTACGATTGGTTCGCTGCAGGGTATAATGGAGCGTGTATGTGCTCTTCCCTCAGACCTGGTTTACCAGGTGGTTGAGTTGGCTCTCCATCTTCTTGAGTGCTCACAGGATCAAGCCAGGAAAAATACAGCCTTATTTTTTTATGCTGCATTTGTTTTCAAGGCAGTTCTTGATGCTTTTGATGCTCAGGATGGCTTACAGAAAATACTGGGCCTTTTAAATGATGCTGCATCAGTAAGATCTGGAGTAAATTCTGGGACATTAGGACTCTCGACATCAGGCTCACTTCGAAATGATAGGTCACAGACAGAAGTTCTGACCTCATCAGAGAAACAGATCGCTTATCACACCTGTGTTGCTTTGCGTCAGTACTTTAGAGCTCATTTGATTTTGCTTGTGGATTCTCTTCGTCCAAATAAAAACAGTCGAAGTGCGGCTCGAAATCTTCCTAGTGTAAGGGCAGCTTACAAGCCACTTGACCTTAGCAACGAAGCTATTGATGCAGTATTTCTGCAGTTACAGAAGGACCGGAAACTCGGTCCTGCATTTGTAAGAACTCGTTGGCCTGCAGTTGATAGGTTTTTGGGATATAATGGACATATTACCATGTTGGAGTTATGCCAGGCCCCACCTGTTGAGCGCTATCTACATGATTTACTTCAATATGCATTGGGTGTTCTGCACATTGTTACATTGGTACCTAGCAGTCGCAAGATGATTGTGAATTCAACATTAAGCAACAATCGTGTTGGTATAGCAGTTATATTGGATGCAGCAAGTGTTAATGGTAGTTATGTGGACCCCGAGATCATTCAGCCAGCTCTAAATGTGTTAGTCAATCTCGTTTGTCCTCCACCTTCAATCAGCAACAAACCACCTTTACTTGCACAAAGTCAGCAGTCTGTTTCTGCCCCAACCTCAAATGCCCTTGCTATAGAGACTAGAGAGAAAAATACTGAACGTAATGTCTCAGATCGAGCTGGGGAATCAGCTGTTGTAGATCGAGGCAGTGTAGCAGCACTGGGTACTGGTACACAGTCAAACAGTAGCAATGCACTATCCTCTACCCCCACAGCAACTTCTGGATTGGTGGGGGATCGCAGGATATCTTTAGGAGTTGGGGCAGGTTGTGCTGGGCTTGCGGCACAACTGGAGCAAGGCTATCGGCAAGCAAGAGAGGCTGTTCGCTCCACCAACGGTATAAAGGTTCTGCTGCATCTTCTCCAACCACGCATATATTCACCTCCTGCCGCACTCGATTGTCTACGTGCGCTTGCCTGCCGAGTCCTTCTTGGTTTAGCGAGAGATGATACAATTGCACACATATTGACAAAGCTCCAGGTTGGAAAGAAACTATCAGAACTAATTCGAGATCAAACACAAGGAGCTGAGCAAGGCAGGTGGCAATCTGAACTTTCACAGGCAGCAATCGAGTTAATGGCGATTGTGACGAATTCTGGACGTGCTAGCACATTGGCTGCCACTGATGCCGCTATGCCAACTTTGAGGCGCATAGAACGGGCAGCTATAGCTGCTGCCACTCCTATCACTTACCATTCCAGGGAGCTTTTGCTTCTAATACATGAACACCTACAGGCATCTGGTTTGGCCACAACAGCTGCTTCACTGCTAAGAGAGGCCCAGTTGATGCCTTTGCCATCCTTGGCTGCCCCCTCATCTCTTGTTCATCAAGCTACACAAGAAGCCTCATCTCTGCAGCTTCAGTGGCCCTCTGGTCGCACCCCTATTGGATTTCTTACCAACAAATCAAAGATAGCAAGGGAGGAGGATTCAAGCTTGAAATGTGATTCTTCTGTCTCATACTCAAAGAAAAAACCTTTGATTTTCTCTCCAAGTTTTTGTCTTCAGTCAAGGAATCCGTTCCAGTCCCATGACTCTCACCCAACATTGGCCACAAATGTCTTAAGTGCATCGAAAGAGTTATCTGCACCTGCAAATGCATCAGAAGCTCCGTCAGATATCCTGCCGAAACCTAGTATTGATACAGAGTACCAGTGTAAGACACCCATCCTGTTGCCGATGAAAAGAAAGTTATCCGAGTTAAAGGATCCTGGATGTTTGCCATCATCTGGGAAACGCATCCACACCGGGGACCAGGGATATCGGTCTCCAATTTTTCCCACACCCAATATTGTGCGGAAAAGCGGTCTACTAACTGATCTGGCTGGGTTCTCTAGTCCTACTTCCAACATGAGAGATCAACATTGTCGATCCACACCTGCTTGTTTTTCATCAGAGTACCTGGATGATAACCAGTATGGTAATTCTAGTGTAGGTCTGGCAACACCATCCTCCCAACTTGGGCTTCAAAACGACCCTCAACCAAGCAACCCAGAAAGATTGACTCTGGACTCTTTGGTTGTTCAGTATCTGAAGCACCAGCATCGCCAGTGTCCTGCCCCTATAACCACTCTTCCACCACTGTCCCTTTTACAGCCACATGTTTGTCCCGAACCAAGACGAACCCTTGATGCCCCTTCAAATGTAACTGCCCGGCTTGGCACCCGTGAGTTCAGAAGTATGTATGGTGGGGTGCATGGAAACCGCAGGGATCGTCAGTTTGTTTACAGTAGATTCCGGCCATGGCGGACTTGTAGGGATGGTACTGGTACCCCTTTGACATGCATCTCTTTTCTCAGTGACACTGCTCATATTGCAGTAGGCAGTCATGGTGGAGAGCTCAAAATTTTTGATTCCAATAGCAGCAATGTATTGGAGAGTTGTCCAAGCCACCAGTCTCCTGTAACGCTTGTTCAGACATATCATTCAGGTGAAACCGAATTGGTGCTGTCTTCTAGTTCAGAAGATGTGAGGTTGTGGGATGCATCTACAGTCTCATCTGGGCCAATGCATTCATATGAAGGGTGCAAGGCTGCAAGATTTAGCAATTCTGGGGATGTTTTTGCAGCCCTATCATCAGAGCTGGCGCAAAAAGAAATTCTAATATATGACATCCAAACTAACCGGTTGGAATCAAAACTGTCAGATACAGCTTCTAGCACGGGACGTGGACATTCATATTCTCATATACACTTCAACCCTTCAGACACAATGCTGCTCTGGAATGGTGTCCTATGGGACAGGCGGGTTTCCAGCCCTGTTCATCGTTTTGATCAGTTTACGGATTATGGGGGTGGTGGCTTCCATCCTGCTGGGAACGAGGTGATTATAAACTCTGAGGTCTGGGATCTCCGGAACTTTAGACTCCTCCGAAGTGTACCTTCTTTAGACCAAACTACAATAACTTTTAATGCCCGTGGTGATGTAATTTATGCAATCCTCCGGAGAAATCTTGATGATGTAATGTCGGCAGTGAATACCCGTCGCGTAAAGCATCCTCTCTTTGCTGCTTTCCGCACGGTGGATGCGGTTAACTACTCGGACATTGCCACGATTCCGGTTGATCGTTGTGTCCTCGACTTTGCAACAGAGCCAACTGATTCATTTCTTGGGCTGATCACAATGGATGACCAAGATGAGATGTTTGCTTCTGCTAGGGTTTATGAAATTGGTCGCCGAAAGCCGACAGATGATGATTCTGATCCTGATGATGCCGAGAGTGAAGAGGACGATGATGATGAAGAAGACGACGACGATGCGGATATGGACACTTTACTAGGCCCTGATCTTGACGGGGATGATGATAGTGATATGAGTAATGATGATGATGATGATGATAGTGGGAGCGACCTTGATGAGGATGATGAGGGAGATTATATGATGGATGGGGACTTTGATGGAGGAGGTGGAATGCTTGAGATTTTGACAGACGGTGAGGAAGATGACGATGACAGTGATGGACTGGTTGAATCCTTTAGCAGTGGTGATGAGGAAGATTTTGCAGGCCATGGTTTCGGTTATTAA

Protein Analysis

1960

Amino Acids

213.56

Weight (kDa)

4.94

Isoelectric Point (pI)

50.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 1543 - 1617 5.3e-07 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 1544 - 1619 1.8e-07 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 1545 - 1670 1.4e-08 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 1547 - 1692 9e-08 THOC3 beta-propeller domain
WD40_Prp19 PF24814 1547 - 1714 7.9e-06 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013230)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31160
fragaria_vesca FvH4_2g30560
malus_domestica MD08G1132100.v1.1 MD15G1113300.v1.1
prunus_persica Prupe.1G463700_v2.0.a1
pyrus_communis pycom08g11060 pycom15g10160
rosa_chinensis RchiOBHm_Chr6g0312521
rosa_laevigata RLG00000010322
rosa_multiflora Rmu_sc0003094.1_g000015 Rmu_sc0035272.1_g000001
rosa_roxburghii Rroxscaffold_7G00156780
rosa_rugosa Rorug06G0396000
rosa_samantha Rh6AG513400 Rh6BG526600 Rh6CG531500 Rh6DG515500
rosa_wichuraiana Rw6G044640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 5189
AarI CACCTGC 3 cut(s) 3387, 3958, 4246
AatII GACGTC 1 cut(s) 677
Acc36I ACCTGC 5 cut(s) 814, 3113, 3387, 3958, 4246
Acc65I GGTACC 2 cut(s) 2695, 4628
AccB1I GGYRCC 4 cut(s) 85, 2695, 4520, 4628
AccB7I CCANNNNNTGG 2 cut(s) 971, 4088
AccI GTMKAC 3 cut(s) 1791, 3262, 4168
AccII CGCG 2 cut(s) 59, 5346
AccIII TCCGGA 3 cut(s) 677, 5211, 5301
AclI AACGTT 1 cut(s) 943
AcoI YGGCCR 4 cut(s) 165, 3606, 3909, 4598
AcuI CTGAAG 6 cut(s) 623, 1425, 3704, 3848, 4412, 5046
AcyI GRCGYC 2 cut(s) 86, 674
AfeI AGCGCT 1 cut(s) 2639
AflII CTTAAG 1 cut(s) 3922
AflIII ACRYGT 1 cut(s) 4459
AgeI ACCGGT 1 cut(s) 4983
AjiI CACGTC 3 cut(s) 1246, 3461, 5027
AjuI GAANNNNNNNTTGG 4 cut(s) 551, 583, 4308, 4340
AleI CACNNNNGTG 1 cut(s) 2349
Alw21I GWGCWC 5 cut(s) 1888, 2044, 2102, 2380, 4701
AlwNI CAGNNNCTG 5 cut(s) 428, 829, 1403, 1945, 4390
Ama87I CYCGRG 1 cut(s) 2801
Aor13HI TCCGGA 3 cut(s) 677, 5211, 5301
Aor51HI AGCGCT 1 cut(s) 2639
AseI ATTAAT 1 cut(s) 371
AsiGI ACCGGT 1 cut(s) 4983
Asp700I GAANNNNTTC 2 cut(s) 3363, 3878
Asp718I GGTACC 2 cut(s) 2695, 4628
AspLEI GCGC 7 cut(s) 61, 88, 1092, 2640, 3271, 3510, 4948
AsuC2I CCSGG 4 cut(s) 83, 1179, 4108, 4513
AsuII TTCGAA 1 cut(s) 2287
AsuNHI GCTAGC 1 cut(s) 3464
AvaI CYCGRG 1 cut(s) 2801
AvaII GGWCC 6 cut(s) 883, 1766, 2524, 2537, 2797, 4111
BaeGI GKGCMC 1 cut(s) 1114
BaeI ACNNNNGTAYC 4 cut(s) 898, 931, 2679, 2712
BalI TGGCCA 3 cut(s) 167, 3608, 3911
BamHI GGATCC 1 cut(s) 4066
BanI GGYRCC 4 cut(s) 85, 2695, 4520, 4628
BanII GRGCYC 2 cut(s) 2380, 4701
BarI GAAGNNNNNNTAC 2 cut(s) 2028, 2060
BauI CACGAG 1 cut(s) 1020
BbsI GAAGAC 8 cut(s) 126, 135, 156, 492, 611, 3854, 4803, 5610
Bbv12I GWGCWC 5 cut(s) 1888, 2044, 2102, 2380, 4701
BceAI ACGGC 1 cut(s) 344
BcgI CGANNNNNNTGC 8 cut(s) 1502, 1536, 3143, 3177, 4385, 4419, 5597, 5631
BclI TGATCA 2 cut(s) 5131, 5475
BcnI CCSGG 4 cut(s) 83, 1179, 4108, 4513
BfoI RGCGCY 2 cut(s) 89, 2641
BfrI CTTAAG 1 cut(s) 3922
BfuAI ACCTGC 5 cut(s) 814, 3113, 3387, 3958, 4246
BglII AGATCT 1 cut(s) 2237
BlpI GCTNAGC 1 cut(s) 3387
BmcAI AGTACT 1 cut(s) 2369
Bme18I GGWCC 6 cut(s) 883, 1766, 2524, 2537, 2797, 4111
BmeT110I CYCGRG 1 cut(s) 2801
BmgBI CACGTC 3 cut(s) 1246, 3461, 5027
BmrI ACTGGG 5 cut(s) 520, 651, 2216, 3029, 3634
BmtI GCTAGC 1 cut(s) 3468
BmuI ACTGGG 5 cut(s) 520, 651, 2216, 3029, 3634
BoxI GACNNNNGTC 1 cut(s) 1797
BpiI GAAGAC 8 cut(s) 126, 135, 156, 492, 611, 3854, 4803, 5610
BpmI CTGGAG 3 cut(s) 2262, 3170, 3320
Bpu10I CCTNAGC 2 cut(s) 2184, 2480
Bpu1102I GCTNAGC 1 cut(s) 3387
Bpu14I TTCGAA 1 cut(s) 2287
BpuEI CTTGAG 4 cut(s) 1069, 1754, 2114, 5795
BpuMI CCSGG 4 cut(s) 83, 1179, 4108, 4513
BsaAI YACGTR 2 cut(s) 1031, 3266
BsaBI GATNNNNATC 1 cut(s) 5559
BsaHI GRCGYC 2 cut(s) 86, 674
BsaI GGTCTC 2 cut(s) 589, 4131
BsaWI WCCGGW 7 cut(s) 677, 1899, 2526, 4983, 5211, 5301, 5416
BsaXI ACNNNNNCTCC 4 cut(s) 1737, 1767, 1779, 1809
Bse118I RCCGGY 1 cut(s) 4983
Bse3DI GCAATG 5 cut(s) 307, 1857, 3055, 4732, 5403
Bse8I GATNNNNATC 1 cut(s) 5559
BseAI TCCGGA 3 cut(s) 677, 5211, 5301
BseJI GATNNNNATC 1 cut(s) 5559
BseMI GCAATG 5 cut(s) 307, 1857, 3055, 4732, 5403
BseMII CTCAG 9 cut(s) 188, 434, 600, 2064, 2198, 2982, 3378, 4669, 5187
BseRI GAGGAG 8 cut(s) 122, 149, 293, 951, 1072, 3797, 5216, 5775
BseSI GKGCMC 1 cut(s) 1114
BseYI CCCAGC 5 cut(s) 215, 2978, 3131, 4184, 5171
BsgI GTGCAG 3 cut(s) 1500, 2663, 3930
Bsh1236I CGCG 2 cut(s) 59, 5346
Bsh1285I CGRYCG 1 cut(s) 543
BshNI GGYRCC 4 cut(s) 85, 2695, 4520, 4628
BshTI ACCGGT 1 cut(s) 4983
BsiEI CGRYCG 1 cut(s) 543
BsiHKAI GWGCWC 5 cut(s) 1888, 2044, 2102, 2380, 4701
BsiHKCI CYCGRG 1 cut(s) 2801
BsmI GAATGC 4 cut(s) 1291, 1600, 4870, 5775
Bso31I GGTCTC 2 cut(s) 589, 4131
BsoBI CYCGRG 1 cut(s) 2801
Bsp119I TTCGAA 1 cut(s) 2287
Bsp1286I GDGCHC 6 cut(s) 1114, 1888, 2044, 2102, 2380, 4701
Bsp13I TCCGGA 3 cut(s) 677, 5211, 5301
Bsp1720I GCTNAGC 1 cut(s) 3387
Bsp19I CCATGG 3 cut(s) 12, 4601, 5866
BspCNI CTCAG 9 cut(s) 187, 435, 599, 2063, 2197, 2981, 3379, 4668, 5188
BspEI TCCGGA 3 cut(s) 677, 5211, 5301
BspFNI CGCG 2 cut(s) 59, 5346
BspMAI CTGCAG 6 cut(s) 1399, 1525, 2019, 2514, 2569, 3717
BspMI ACCTGC 5 cut(s) 814, 3113, 3387, 3958, 4246
BspOI GCTAGC 1 cut(s) 3468
BspQI GCTCTTC 1 cut(s) 2049
BspT104I TTCGAA 1 cut(s) 2287
BspT107I GGYRCC 4 cut(s) 85, 2695, 4520, 4628
BspTI CTTAAG 1 cut(s) 3922
BspTNI GGTCTC 2 cut(s) 589, 4131
BsrDI GCAATG 5 cut(s) 307, 1857, 3055, 4732, 5403
BsrFI RCCGGY 1 cut(s) 4983
BssAI RCCGGY 1 cut(s) 4983
BssNI GRCGYC 2 cut(s) 86, 674
BssSI CACGAG 1 cut(s) 1020
BssT1I CCWWGG 7 cut(s) 12, 46, 168, 854, 3660, 4601, 5866
Bst2BI CACGAG 1 cut(s) 1020
Bst6I CTCTTC 4 cut(s) 2049, 2409, 4437, 5577
BstACI GRCGYC 2 cut(s) 86, 674
BstAFI CTTAAG 1 cut(s) 3922
BstAPI GCANNNNNTGC 4 cut(s) 428, 1421, 1945, 3128
BstBAI YACGTR 2 cut(s) 1031, 3266
BstBI TTCGAA 1 cut(s) 2287
BstDSI CCRYGG 5 cut(s) 12, 1102, 4601, 5275, 5866
BstENI CCTNNNNNAGG 1 cut(s) 5714
BstFNI CGCG 2 cut(s) 59, 5346
BstH2I RGCGCY 2 cut(s) 89, 2641
BstHHI GCGC 7 cut(s) 61, 88, 1092, 2640, 3271, 3510, 4948
BstMCI CGRYCG 1 cut(s) 543
BstNSI RCATGY 3 cut(s) 1939, 4463, 4644
BstPAI GACNNNNGTC 1 cut(s) 1797
BstSLI GKGCMC 1 cut(s) 1114
BstUI CGCG 2 cut(s) 59, 5346
BstV2I GAAGAC 8 cut(s) 126, 135, 156, 492, 611, 3854, 4803, 5610
BstX2I RGATCY 4 cut(s) 1181, 2237, 4066, 5206
BstXI CCANNNNNNTGG 1 cut(s) 5171
BstYI RGATCY 4 cut(s) 1181, 2237, 4066, 5206
BtgI CCRYGG 5 cut(s) 12, 1102, 4601, 5275, 5866
BtgZI GCGATG 1 cut(s) 4388
BtrI CACGTC 3 cut(s) 1246, 3461, 5027
BtsI GCAGTG 6 cut(s) 1018, 3013, 3623, 4662, 5337, 5845
BveI ACCTGC 5 cut(s) 814, 3113, 3387, 3958, 4246
CaiI CAGNNNCTG 5 cut(s) 428, 829, 1403, 1945, 4390
CfoI GCGC 7 cut(s) 61, 88, 1092, 2640, 3271, 3510, 4948
Cfr10I RCCGGY 1 cut(s) 4983
CseI GACGC 2 cut(s) 399, 2351
CsiI ACCWGGT 2 cut(s) 2055, 2064
CspAI ACCGGT 1 cut(s) 4983
CspCI CAANNNNNGTGG 2 cut(s) 3380, 3415
DinI GGCGCC 1 cut(s) 87
DraI TTTAAA 1 cut(s) 2217
EaeI YGGCCR 4 cut(s) 165, 3606, 3909, 4598
Eam1104I CTCTTC 4 cut(s) 2049, 2409, 4437, 5577
EarI CTCTTC 4 cut(s) 2049, 2409, 4437, 5577
EciI GGCGGA 2 cut(s) 60, 4621
Ecl136II GAGCTC 2 cut(s) 2378, 4699
Eco130I CCWWGG 7 cut(s) 12, 46, 168, 854, 3660, 4601, 5866
Eco24I GRGCYC 2 cut(s) 2380, 4701
Eco31I GGTCTC 2 cut(s) 589, 4131
Eco32I GATATC 4 cut(s) 1363, 3105, 3979, 4121
Eco47I GGWCC 6 cut(s) 883, 1766, 2524, 2537, 2797, 4111
Eco47III AGCGCT 1 cut(s) 2639
Eco53kI GAGCTC 2 cut(s) 2378, 4699
Eco57I CTGAAG 6 cut(s) 623, 1425, 3704, 3848, 4412, 5046
Eco88I CYCGRG 1 cut(s) 2801
EcoICRI GAGCTC 2 cut(s) 2378, 4699
EcoNI CCTNNNNNAGG 1 cut(s) 5714
EcoO109I RGGNCCY 2 cut(s) 2623, 5639
EcoRI GAATTC 4 cut(s) 1237, 1493, 2722, 3451
EcoRV GATATC 4 cut(s) 1363, 3105, 3979, 4121
EcoT14I CCWWGG 7 cut(s) 12, 46, 168, 854, 3660, 4601, 5866
EcoT22I ATGCAT 5 cut(s) 2668, 3961, 4646, 4846, 4872
EcoT38I GRGCYC 2 cut(s) 2380, 4701
EgeI GGCGCC 1 cut(s) 87
EheI GGCGCC 1 cut(s) 87
ErhI CCWWGG 7 cut(s) 12, 46, 168, 854, 3660, 4601, 5866
FalI AAGNNNNNCTT 4 cut(s) 2446, 2478, 5224, 5256
FauI CCCGC 1 cut(s) 5097
FauNDI CATATG 2 cut(s) 1880, 4876
FbaI TGATCA 2 cut(s) 5131, 5475
FblI GTMKAC 3 cut(s) 1791, 3262, 4168
FriOI GRGCYC 2 cut(s) 2380, 4701
GlaI GCGC 7 cut(s) 60, 87, 1091, 2639, 3270, 3509, 4947
GsaI CCCAGC 5 cut(s) 219, 2982, 3135, 4188, 5175
GsuI CTGGAG 3 cut(s) 2262, 3170, 3320
HaeII RGCGCY 2 cut(s) 89, 2641
HgaI GACGC 2 cut(s) 399, 2351
HhaI GCGC 7 cut(s) 61, 88, 1092, 2640, 3271, 3510, 4948
Hin1I GRCGYC 2 cut(s) 86, 674
Hin6I GCGC 7 cut(s) 59, 86, 1090, 2638, 3269, 3508, 4946
HinP1I GCGC 7 cut(s) 59, 86, 1090, 2638, 3269, 3508, 4946
HincII GTYRAC 3 cut(s) 672, 1170, 5392
HindII GTYRAC 3 cut(s) 672, 1170, 5392
HindIII AAGCTT 2 cut(s) 1210, 3793
HpaI GTTAAC 1 cut(s) 5392
Hpy99I CGWCG 6 cut(s) 23, 128, 1843, 5346, 5612, 5615
Hsp92I GRCGYC 2 cut(s) 86, 674
HspAI GCGC 7 cut(s) 59, 86, 1090, 2638, 3269, 3508, 4946
KasI GGCGCC 1 cut(s) 85
Kpn2I TCCGGA 3 cut(s) 677, 5211, 5301
KpnI GGTACC 2 cut(s) 2699, 4632
Ksp22I TGATCA 2 cut(s) 5131, 5475
KspAI GTTAAC 1 cut(s) 5392
LguI GCTCTTC 1 cut(s) 2049
MabI ACCWGGT 2 cut(s) 2055, 2064
MaeIII GTNAC 8 cut(s) 859, 2298, 2514, 2686, 3445, 4504, 4658, 4762
MfeI CAATTG 2 cut(s) 1575, 3312
MflI RGATCY 4 cut(s) 1181, 2237, 4066, 5206
MhlI GDGCHC 6 cut(s) 1114, 1888, 2044, 2102, 2380, 4701
MlsI TGGCCA 3 cut(s) 167, 3608, 3911
MluNI TGGCCA 3 cut(s) 167, 3608, 3911
Mly113I GGCGCC 1 cut(s) 86
MlyI GAGTC 9 cut(s) 737, 1073, 1745, 2259, 3291, 3887, 4357, 4364, 5217
MmeI TCCRAC 6 cut(s) 1330, 2589, 3247, 3322, 4230, 4968
Mox20I TGGCCA 3 cut(s) 167, 3608, 3911
Mph1103I ATGCAT 5 cut(s) 2668, 3961, 4646, 4846, 4872
MroI TCCGGA 3 cut(s) 677, 5211, 5301
MroXI GAANNNNTTC 2 cut(s) 3363, 3878
MscI TGGCCA 3 cut(s) 167, 3608, 3911
MslI CAYNNNNRTG 5 cut(s) 846, 1659, 2349, 4788, 4875
Msp20I TGGCCA 3 cut(s) 167, 3608, 3911
MspA1I CMGCKG 4 cut(s) 425, 1165, 2990, 3617
MspCI CTTAAG 1 cut(s) 3922
MunI CAATTG 2 cut(s) 1575, 3312
Mva1269I GAATGC 4 cut(s) 1291, 1600, 4870, 5775
MvnI CGCG 2 cut(s) 59, 5346
NarI GGCGCC 1 cut(s) 86
NciI CCSGG 4 cut(s) 83, 1179, 4108, 4513
NcoI CCATGG 3 cut(s) 12, 4601, 5866
NdeI CATATG 2 cut(s) 1880, 4876
NheI GCTAGC 1 cut(s) 3464
NmeAIII GCCGAG 2 cut(s) 3305, 5599
NmuCI GTSAC 3 cut(s) 2298, 3445, 4658
NsiI ATGCAT 5 cut(s) 2668, 3961, 4646, 4846, 4872
NspI RCATGY 3 cut(s) 1939, 4463, 4644
NspV TTCGAA 1 cut(s) 2287
OliI CACNNNNGTG 1 cut(s) 2349
PaeI GCATGC 1 cut(s) 1939
PaqCI CACCTGC 3 cut(s) 3387, 3958, 4246
PasI CCCWGGG 1 cut(s) 34
PciI ACATGT 1 cut(s) 4459
PciSI GCTCTTC 1 cut(s) 2049
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 1027
PctI GAATGC 4 cut(s) 1291, 1600, 4870, 5775
PdmI GAANNNNTTC 2 cut(s) 3363, 3878
PflMI CCANNNNNTGG 2 cut(s) 971, 4088
PfoI TCCNGGA 2 cut(s) 1335, 4069
PinAI ACCGGT 1 cut(s) 4983
PleI GAGTC 9 cut(s) 736, 1073, 1744, 2259, 3290, 3887, 4357, 4364, 5217
PluTI GGCGCC 1 cut(s) 89
PpsI GAGTC 9 cut(s) 736, 1073, 1744, 2259, 3290, 3887, 4357, 4364, 5217
Ppu21I YACGTR 2 cut(s) 1031, 3266
PscI ACATGT 1 cut(s) 4459
PshAI GACNNNNGTC 1 cut(s) 1797
PshBI ATTAAT 1 cut(s) 371
PsiI TTATAA 1 cut(s) 5189
Psp124BI GAGCTC 2 cut(s) 2380, 4701
Psp1406I AACGTT 1 cut(s) 943
PspFI CCCAGC 5 cut(s) 215, 2978, 3131, 4184, 5171
PsrI GAACNNNNNNTAC 2 cut(s) 4755, 4787
PstI CTGCAG 6 cut(s) 1399, 1525, 2019, 2514, 2569, 3717
PstNI CAGNNNCTG 5 cut(s) 428, 829, 1403, 1945, 4390
PsuI RGATCY 4 cut(s) 1181, 2237, 4066, 5206
PvuII CAGCTG 4 cut(s) 425, 1165, 2990, 3617
RseI CAYNNNNRTG 5 cut(s) 846, 1659, 2349, 4788, 4875
SacI GAGCTC 2 cut(s) 2380, 4701
SapI GCTCTTC 1 cut(s) 2049
ScaI AGTACT 1 cut(s) 2369
SchI GAGTC 9 cut(s) 737, 1073, 1745, 2259, 3291, 3887, 4357, 4364, 5217
SduI GDGCHC 6 cut(s) 1114, 1888, 2044, 2102, 2380, 4701
SexAI ACCWGGT 2 cut(s) 2055, 2064
SfoI GGCGCC 1 cut(s) 87
SfuI TTCGAA 1 cut(s) 2287
SinI GGWCC 6 cut(s) 883, 1766, 2524, 2537, 2797, 4111
SmiMI CAYNNNNRTG 5 cut(s) 846, 1659, 2349, 4788, 4875
SmlI CTYRAG 5 cut(s) 1084, 1733, 2093, 3922, 5774
SmoI CTYRAG 5 cut(s) 1084, 1733, 2093, 3922, 5774
SphI GCATGC 1 cut(s) 1939
SspDI GGCGCC 1 cut(s) 85
SspI AATATT 1 cut(s) 4150
SstI GAGCTC 2 cut(s) 2380, 4701
StyI CCWWGG 7 cut(s) 12, 46, 168, 854, 3660, 4601, 5866
TaqII GACCGA 3 cut(s) 529, 2525, 4113
TatI WGTACW 2 cut(s) 1226, 2367
TauI GCSGC 8 cut(s) 11, 59, 76, 1568, 2435, 3143, 3251, 3491
TseFI GTSAC 3 cut(s) 2298, 3445, 4658
Tsp45I GTSAC 3 cut(s) 2298, 3445, 4658
TspGWI ACGGA 5 cut(s) 403, 704, 3867, 3960, 5156
Van91I CCANNNNNTGG 2 cut(s) 971, 4088
Vha464I CTTAAG 1 cut(s) 3922
VpaK11BI GGWCC 6 cut(s) 883, 1766, 2524, 2537, 2797, 4111
VspI ATTAAT 1 cut(s) 371
XagI CCTNNNNNAGG 1 cut(s) 5714
XbaI TCTAGA 1 cut(s) 1118
XceI RCATGY 3 cut(s) 1939, 4463, 4644
XmiI GTMKAC 3 cut(s) 1791, 3262, 4168
XmnI GAANNNNTTC 2 cut(s) 3363, 3878
ZraI GACGTC 1 cut(s) 675
ZrmI AGTACT 1 cut(s) 2369
Zsp2I ATGCAT 5 cut(s) 2668, 3961, 4646, 4846, 4872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.