Rroxscaffold_7G00156780

DDB1- and CUL4-associated factor homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013230
Physical Location & Seq
Reverse (-)
688227 .. 698055
9829 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00156780.1

Sequence Viewer

Length: 5877 bp
ATGGAGGCCGCCATGGACGACGAGCTACAGACTCAGGGCCAGGGCCAAGGCCAAGCCGCGCCACCAGCTCCGCCTCCTCCCCCGGCGCCGGAATCGCAGGCAGGCGAGGAGCCGGAGGAAGACGACGAAGACGAGGAGCCGAAGAATGAAGACGATGAAATGGTGGCCAAGGCTCAGAAGCTCATGGATAAGATTATTGCTTCGCCTGATAACCCCAGCCCCACTGTTCTTCACGCCCTCGCTTCACTTCTTGAAACGCAGGAGAAACGATACATGGAGGAGAGTGGTCATTCTTCCAGCAATGGTCGTGCTTCTCATAACATTGGACGGCTAGGGAACTTGGTTCGGGAACATGATGACTTCTTTGAATTAATATCTGCCAAGTATTTGTCAGATACGAGATACTCTGCGTCCGTCCAGGCAGCTGCTGCAAGGCTGTTTCTGAGCTGTTCACTGACTTTGATTTATCCTCATGTTTTTGAAGAAGACGTTTTGGAGAAAATAAAGGACTGGGTGATGGATGAGACTTCCAGTGTCTCGGTCGAATACCAGAACTGGAAGCATGATTTGGGAGGAAAGGAGGTCTCAGATTTTGAAATGCTGAAGACATATTCTACTGGACTTCTTGCTGTTTGTTTGACTGGGGGTGGTCAAGTGGTGGAAGATGTGTTGACGTCCGGATTGTCAGCCAAACTTATGCGTTATCTTCGTGTCCGTGTTTTGGGGGAGTCAAGTATTAATCAGAAGGATTCTAGTCATTTAACAGAGACTAAAAACACCTCTGGTGTAAGAGGTAGAGATGAAGGTCGGGGTAGGGTTCGGCAGGTTCTGGAAACAACTCATTTTGATGATCCAAGGGTAACAAGTGAGAGATGCTTGGATGACCAGACTGTTGAAGGGAACCAGGACAGAGGTATCTGTAGGCAAGCGTTTGGAGGAGAACGTTGGGGTGATGGTGGAGAACCACCTGATGGAATGGATGAAGGGGTTGAGATCAATGATGTTGATGGCAGTGATTCTCGTGATTTACGCGATGGGAAACAAAAATTTGGTGATCTTGATGAAAATGGGAGAGATGACTCCTCAAGGCGCAGACCTAACCGTGGATGGGCACGATCTAGAGGGAAGGGAAGGGCAAACGAAGGCGGTGTAGAAAATGAGCAGTCGTTGACCTCACCCGGATCTGCTATTCGGTTGGGACAGGGGCGAAGCTTTAGAGACAAGAGTACATCAAAGAATTCAGACGTGAAAAAAATATCAGATTCTAGGAAGTGTTTGGGCAGGAATGCTTCTGATGTTTTGTTTCTGGAAAGAGAGGATAATGATGAGTGCTTCCAGGACTGCACAGTTGGAAGTAAGGATATCTCTGATCTAGTAAAAAAAGCAGTCAGAGCTGCAGAAGCTGAAGCTAGAGCAGCCAATGCACCTGTAGAAGCCATCAAAGCTGCTGGTGATGCTGCTGCGGAAGTTGTCAAAAGTTCAGCTTTAGAGGAATTCACAACTACAAACAATGAAGAAGCTGCAGTTTTGGCTGCTTCAAGAGCAGCATCTACTGTAATTGATGCCGCCAATTCAATTGAAGCTCTAAGTGCGAATGCAGTAAATACCTCTGCTAGTGCTGAACCAATAACTTCAAGCTCCGAACCTCAAAACCATGAAGATGTTGAAGAATACTTTATTCCAAGTGTTGAATCCCTTGCACAGTTGAGAGAAAAATACTGTATTCAATGTCTTGAGACTCTTGGAGAATATGTTGAAGTTCTTGGACCTGTATTGCATGAGAAGGGAGTAGACGTATGTCTTGCCTTGTTGCAACGAAATTCAAGACATAAAGAACCGTCAAAGGTCGCAATGCTTTTGCCTGATGTAATGAAGCTCATATGTGCTCTGGCTGCTCACCGGAAATTTGCTGCATTGTTTGTGGATAGGGGCGGCATGCAGAAACTGCTTGCTGTCCCTAGAGTTGCTCAAACCTACTTTGGCCTTTCTTCTTGCTTATTTACGATCGGTTCGCTGCAGGGTATAATGGAGCGTGTATGTGCTCTTCCCTCAGACCTGGTTTACCAGGTGGTTGAGTTGGCTCTCCATCTTCTCGAGTGCTCACAGGATCAAGCCAGGAAAAATGCAGCCTTATTTTTTTCTGCTGCATTTGTTTTCAAGGCAGTTCTTGATGCTTTTGATGCTCAGGATGGCTTACAGAAAATACTGGGCCTTTTAAATGATGCTGCATCAGTAAGATCTGGAGTAAATTCTGGGACATTAGGACTCTCGACCTCAGGCTCACTTCGAAATGATAGGTCACCGACAGAAGTTCTGACCTCATCAGAGAAACAGATCGCTTATCACACCTGTGTTGCTTTGCGTCAGTACTTTAGAGCTCATTTGATTTTGCTTGTGGATTCTCTTCGTCCAAATAAAAACAGTCGAAGTGCAGCTCGAAATCTTCCAAGTGTAAGGGCAGCTTACAAGCCACTTGACCTTAGCAACGAAGCTATTGATGCAGTATTTCTGCAGTTACAGAAGGACCGGAAACTCGGTCCTGCATTTGTAAGAACTCGTTGGCCTGCAGTTGATAGGTTTTTGGGATATAATGGACATATTACTATGTTGGAGTTATGCCAGGCCCCACCTGTTGAGCGCTATCTACATGATTTACTTCAATATGCATTGGGTGTTCTGCACATTGTTACATTGGTACCTAGCAGTCGCAAGATGATTGTGAATTCAACATTAAGCAACAATCGTGTTGGTATAGCAGTTATATTGGATGCAGCAAGTGTTAATGGTAGTTATGTGGACCCCGAGATAATTCAGCCAGCTCTAAATGTGTTAGTCAATCTCGTTTGCCCTCCACCTTCAATCAGCAACAAACCACCTTTACTTGCACAAAGTCAGCAGTCTGTTTCTGCCCCAACCTCAAATGCCCTTGCTATAGAGACTAGAGAGAAAAATGCTGAACGTAATGTCTCAGATCGAGCTGGGGAATCAGCTGGTGTAGATCGAGGCAGTGTAGCAGCACTGGGTACTGGTACACAGTCAAACAGTAGCAATGCACTATCCTCTACCCCCACAGCAACTTCTGGATTGGTGGGGGATCGCAGGATATCTTTAGGAGTTGGGGCAGGTTGTGCTGGGCTTGCGGCACAACTGGAGCAAGGCTATCGGCAAGCAAGAGAGGCTGTTCGCTCCACCAACGGTATAAAGGTTCTGCTGCATCTTCTCCAACCACGCATATATTCACCTCCTGCCGCACTCGATTGTCTACGTGCGCTTGCCTGCCGAGTCCTTCTTGGTTTAGCGAGAGATGATACAATTGCACACATATTGACAAAGCTCCAGGTTGGAAAGAAACTATCAGAACTAATTCGAGATCAAACACAAGGAGCTGAGCAAGGCAGGTGGCAATCTGAACTTTCACAGGCAGCAATCGAGTTAATGGCGATTGTGACGAATTCTGGACGTGCTAGCACATTGGCTGCCACTGATGCCGCTATGCCAACTTTGAGGCGCATAGAACGGGCAGCTATAGCTGCTGCCACTCCTATCACTTACCATTCCAGGGAGCTTTTGCTTCTAATACATGAACACCTACAGGCATCTGGTTTGGCCACAACAGCTGCTTCACTGCTAAAAGAGGCCCAGTTGATGCCTTTGCCATCCTTGGCTGCCCCCTCTTCTTTTGTTCATCAAGCTACACAAGAAGCCTCATCTCTGCAGCTTCAGTGGCCCTCTGGTCGCACCCCTATTGGATTTCTTACCAACAAATCAAAGATAGCAAGGGAGGAGGATTCAAGCTTGAAATGTGATTCTACTGTCTCATACTCAAAGAAAAAACCTTTGGTTTTCTCTCCAAGTTTTTGTCTTCAGTCAAGGAATCCGTTCCAGTCCCATGACTCTCACCCAACATTGGCCACAAATGTCTTAAGTGCATCGAAAGAGTTATCTGCACCTGCAAATGCATCAGAAGCTCCATCAGATATCCTGCCGAAACCTAGTATTGATACAGAGCACCAGTGTAAGACACCCATCCTGTTGCCGATAAAAAGAAAGTTATCCGAGTTAAAGGATCCTGGATGTTTGCCATCAGGGAAACGCATCCATACCGGGGACCAGGGATATCGGTCTCCAATTTTTCCCACACCCAATATTGTGCGGAAAAGCGGTCTACTAACTGATCTGGCTGGGTTCTCTACTCCTACTTCCAACATGAGAGATCAACATTGTCGATCCACACCTGCTTGTTTTTCATCAGAGTACCTGGATGATAACCAGTATGGTAATTCTAGTGTAGGTCTGGCAACACCATCCTCCCAACTTGGGCTTCAAAACGACCCTCAACCAAGCAACCCAGAAAGATTGACTCTGGACTCTTTGGTTGTTCAGTATCTGAAGCACCAGCATCGCCAGTGTCCTGCCCCTATAACCACTCTTCCACCACTGTCCCTGTTACAGCCACATGTTTGTCCCGAACCAAGACGAACCCTTGATGCCCCTTCAAATGTAACTGCCCGGCTTGGCACCCGTGAGTTCAGAAGTATGTATGGTGGGGTGCATGGAAACCGCAGGGATCGTCAGTTTGTTTACAGTAGATTCCGGCCATGGCGGACTTGTAGGGATGATACTGGTACCCCTTTGACATGCATCTCTTTTCTGAGTGACACTGCTCATATTGCAGTAGGCAGTCATGGTGGAGAGCTCAAAATTTTTGATTCCAATAGCAGCAATGTATTGGAGAGTTGTCCAAGCCACCAGTCTCCTGTAACGCTTGTTCAGACATATCATTCAGGTGAAACCGAATTGGTGCTGTCTTCTAGTTCAGAAGATGTGAGGTTGTGGGATGCATCTACAGTCTCATCTGGGCCAATGCATTCATATGAAGGGTGCAAGGCTGCAAGATTTAGCAATTCTGGGGATGTTTTTGCATCCCTATCATCAGAGCTGGCACAAAAAGAAATTCTAATATATGACATCCAAACTAACCGGTTGGAATCAAAACTGTCAGATACAGCTGCTAGCACGGGACGTGGACATTCATATTCTCATATACACTTCAACCCTTCAGACACAATGCTGCTCTGGAATGGTGTCCTATGGGACAGGCGGGTTTCCAGCCCTGTTCATCGTTTTGATCAGTTTACGGATTATGGGGGTGGTGGCTTCCATCCTGCTGGGAACGAGGTGATTATAAACTCTGAGGTCTGGGATCTCCGGAACTTTAGACTCCTCCGAAGTGTACCTTCGTTAGACCAAACTACAATAACTTTTAATGCCCGTGGTGATGTAATTTATGCAATCCTCCGGAGAAATCTTGATGATGTAATGTCAGCGGTGAATACCCGTCGTGTAAAGCATCCTCTCTTTGCCGCTTTCCGCACAGTGGATGCGGTTAACTACTCGGACATTGCCACGATTCCGGTTGATCGTTGTGTCCTCGACTTTGCAACAGAGCCAACTGATTCATTTCTTGGGCTGATCACAATGGATGACCAAGATGAGATGTTTGCTTCTGCTAGGGTTTATGAAATTGGTCGCCGAAAGCCGACAGATGATGATTCTGATCCTGATGATGCCGAGAGTGAAGAGGATGATGATGATGAAGAAGATGACGACGATGCGGATATGGACACTCTACTGGGCCCTGATCTTGACGGGGATGATGATAGTGATATGAGTAATGATGATGATGATGATAGTGGAAGCGACCTTGATGACGACGATGAGGGAGATTATATGATGGATGGGGACTTTGATGGAGGAGGTGGAATGCTTGAGATTTTGACAGACGGTGAGGAAGATGACGATGACAGTGATGAACTGGTTGAATCCTTTAGCAGTGGTGATGAGGAAGATTTTGCAGGCCATGGTTTCGGTTATTAA

Protein Analysis

1958

Amino Acids

213.08

Weight (kDa)

4.95

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 1541 - 1661 9.8e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 1543 - 1618 1.8e-07 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 1543 - 1618 3.4e-07 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 1546 - 1691 3.9e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 1546 - 1617 5.3e-06 WDR5 beta-propeller domain
WD40_Prp19 PF24814 1546 - 1743 4.3e-06 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013230)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31160
fragaria_vesca FvH4_2g30560
malus_domestica MD08G1132100.v1.1 MD15G1113300.v1.1
prunus_persica Prupe.1G463700_v2.0.a1
pyrus_communis pycom08g11060 pycom15g10160
rosa_chinensis RchiOBHm_Chr6g0312521
rosa_laevigata RLG00000010322
rosa_multiflora Rmu_sc0003094.1_g000015 Rmu_sc0035272.1_g000001
rosa_roxburghii Rroxscaffold_7G00156780
rosa_rugosa Rorug06G0396000
rosa_samantha Rh6AG513400 Rh6BG526600 Rh6CG531500 Rh6DG515500
rosa_wichuraiana Rw6G044640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 5186
AarI CACCTGC 3 cut(s) 3387, 3958, 4243
AatII GACGTC 1 cut(s) 677
Acc36I ACCTGC 5 cut(s) 814, 3113, 3387, 3958, 4243
Acc65I GGTACC 2 cut(s) 2695, 4625
AccB1I GGYRCC 4 cut(s) 85, 2695, 4517, 4625
AccB7I CCANNNNNTGG 1 cut(s) 971
AccI GTMKAC 3 cut(s) 1791, 3262, 4165
AccII CGCG 2 cut(s) 59, 1032
AccIII TCCGGA 3 cut(s) 677, 5208, 5298
AclI AACGTT 1 cut(s) 943
AcoI YGGCCR 4 cut(s) 165, 3606, 3909, 4595
AcuI CTGAAG 6 cut(s) 623, 1425, 3704, 3848, 4409, 5043
AcyI GRCGYC 2 cut(s) 86, 674
AfaI GTAC 8 cut(s) 1228, 2369, 2697, 3025, 3031, 4256, 4627, 5235
AfeI AGCGCT 1 cut(s) 2639
AflII CTTAAG 1 cut(s) 3922
AflIII ACRYGT 1 cut(s) 4456
AgeI ACCGGT 1 cut(s) 4980
AjiI CACGTC 3 cut(s) 1246, 3461, 5024
AjuI GAANNNNNNNTTGG 6 cut(s) 551, 583, 3821, 3853, 4305, 4337
AleI CACNNNNGTG 1 cut(s) 2349
Alw21I GWGCWC 6 cut(s) 1888, 2044, 2102, 2380, 4011, 4698
AlwNI CAGNNNCTG 5 cut(s) 428, 829, 1403, 1945, 4387
Ama87I CYCGRG 2 cut(s) 2093, 2801
Aor13HI TCCGGA 3 cut(s) 677, 5208, 5298
Aor51HI AGCGCT 1 cut(s) 2639
ApaI GGGCCC 1 cut(s) 5639
AseI ATTAAT 2 cut(s) 371, 738
AsiGI ACCGGT 1 cut(s) 4980
Asp700I GAANNNNTTC 2 cut(s) 3363, 3878
Asp718I GGTACC 2 cut(s) 2695, 4625
AspLEI GCGC 6 cut(s) 61, 88, 1092, 2640, 3271, 3510
AsuC2I CCSGG 4 cut(s) 83, 1179, 4105, 4510
AsuII TTCGAA 1 cut(s) 2287
AsuNHI GCTAGC 2 cut(s) 3464, 5012
AvaI CYCGRG 2 cut(s) 2093, 2801
AvaII GGWCC 5 cut(s) 1766, 2524, 2537, 2797, 4108
AxyI CCTNAGG 1 cut(s) 2275
BaeGI GKGCMC 2 cut(s) 1114, 5639
BaeI ACNNNNGTAYC 4 cut(s) 898, 931, 2679, 2712
BalI TGGCCA 3 cut(s) 167, 3608, 3911
BamHI GGATCC 1 cut(s) 4066
BanI GGYRCC 4 cut(s) 85, 2695, 4517, 4625
BanII GRGCYC 3 cut(s) 2380, 4698, 5639
BarI GAAGNNNNNNTAC 2 cut(s) 2028, 2060
BauI CACGAG 1 cut(s) 1020
BbsI GAAGAC 7 cut(s) 126, 135, 156, 492, 611, 3854, 4800
Bbv12I GWGCWC 6 cut(s) 1888, 2044, 2102, 2380, 4011, 4698
BceAI ACGGC 1 cut(s) 344
BcgI CGANNNNNNTGC 4 cut(s) 3143, 3177, 4382, 4416
BclI TGATCA 2 cut(s) 5128, 5472
BcnI CCSGG 4 cut(s) 83, 1179, 4105, 4510
BfoI RGCGCY 2 cut(s) 89, 2641
BfrI CTTAAG 1 cut(s) 3922
BfuAI ACCTGC 5 cut(s) 814, 3113, 3387, 3958, 4243
BglII AGATCT 1 cut(s) 2237
BlpI GCTNAGC 1 cut(s) 3387
BmcAI AGTACT 1 cut(s) 2369
Bme18I GGWCC 5 cut(s) 1766, 2524, 2537, 2797, 4108
BmeT110I CYCGRG 2 cut(s) 2093, 2801
BmgBI CACGTC 3 cut(s) 1246, 3461, 5024
BmrI ACTGGG 6 cut(s) 520, 651, 2216, 3029, 3634, 5642
BmtI GCTAGC 2 cut(s) 3468, 5016
BmuI ACTGGG 6 cut(s) 520, 651, 2216, 3029, 3634, 5642
BoxI GACNNNNGTC 1 cut(s) 1797
BpiI GAAGAC 7 cut(s) 126, 135, 156, 492, 611, 3854, 4800
BpmI CTGGAG 3 cut(s) 2262, 3170, 3320
Bpu10I CCTNAGC 2 cut(s) 2184, 2480
Bpu1102I GCTNAGC 1 cut(s) 3387
Bpu14I TTCGAA 1 cut(s) 2287
BpuEI CTTGAG 3 cut(s) 1069, 1754, 5789
BpuMI CCSGG 4 cut(s) 83, 1179, 4105, 4510
BsaAI YACGTR 1 cut(s) 3266
BsaBI GATNNNNATC 1 cut(s) 5556
BsaHI GRCGYC 2 cut(s) 86, 674
BsaI GGTCTC 2 cut(s) 589, 4128
BsaWI WCCGGW 7 cut(s) 677, 1899, 2526, 4980, 5208, 5298, 5413
BsaXI ACNNNNNCTCC 4 cut(s) 1737, 1767, 1779, 1809
Bse118I RCCGGY 1 cut(s) 4980
Bse21I CCTNAGG 1 cut(s) 2275
Bse3DI GCAATG 5 cut(s) 307, 1857, 3055, 4729, 5400
Bse8I GATNNNNATC 1 cut(s) 5556
BseAI TCCGGA 3 cut(s) 677, 5208, 5298
BseJI GATNNNNATC 1 cut(s) 5556
BseMI GCAATG 5 cut(s) 307, 1857, 3055, 4729, 5400
BseRI GAGGAG 9 cut(s) 66, 122, 149, 293, 951, 1072, 3797, 5213, 5769
BseSI GKGCMC 2 cut(s) 1114, 5639
BseYI CCCAGC 5 cut(s) 215, 2978, 3131, 4181, 5168
BsgI GTGCAG 4 cut(s) 1327, 2451, 2663, 3930
Bsh1236I CGCG 2 cut(s) 59, 1032
Bsh1285I CGRYCG 2 cut(s) 543, 2007
BshNI GGYRCC 4 cut(s) 85, 2695, 4517, 4625
BshTI ACCGGT 1 cut(s) 4980
BsiEI CGRYCG 2 cut(s) 543, 2007
BsiHKAI GWGCWC 6 cut(s) 1888, 2044, 2102, 2380, 4011, 4698
BsiHKCI CYCGRG 2 cut(s) 2093, 2801
BsmI GAATGC 4 cut(s) 1291, 1600, 4867, 5769
Bso31I GGTCTC 2 cut(s) 589, 4128
BsoBI CYCGRG 2 cut(s) 2093, 2801
Bsp119I TTCGAA 1 cut(s) 2287
Bsp120I GGGCCC 1 cut(s) 5635
Bsp1286I GDGCHC 8 cut(s) 1114, 1888, 2044, 2102, 2380, 4011, 4698, 5639
Bsp13I TCCGGA 3 cut(s) 677, 5208, 5298
Bsp1720I GCTNAGC 1 cut(s) 3387
Bsp19I CCATGG 3 cut(s) 12, 4598, 5860
BspEI TCCGGA 3 cut(s) 677, 5208, 5298
BspFNI CGCG 2 cut(s) 59, 1032
BspMAI CTGCAG 6 cut(s) 1399, 1525, 2019, 2514, 2569, 3717
BspMI ACCTGC 5 cut(s) 814, 3113, 3387, 3958, 4243
BspOI GCTAGC 2 cut(s) 3468, 5016
BspQI GCTCTTC 1 cut(s) 2049
BspT104I TTCGAA 1 cut(s) 2287
BspT107I GGYRCC 4 cut(s) 85, 2695, 4517, 4625
BspTI CTTAAG 1 cut(s) 3922
BspTNI GGTCTC 2 cut(s) 589, 4128
BsrDI GCAATG 5 cut(s) 307, 1857, 3055, 4729, 5400
BsrFI RCCGGY 1 cut(s) 4980
BssAI RCCGGY 1 cut(s) 4980
BssNI GRCGYC 2 cut(s) 86, 674
BssSI CACGAG 1 cut(s) 1020
BssT1I CCWWGG 7 cut(s) 12, 46, 168, 854, 3660, 4598, 5860
Bst2BI CACGAG 1 cut(s) 1020
Bst6I CTCTTC 5 cut(s) 2049, 2409, 3679, 4434, 5574
BstACI GRCGYC 2 cut(s) 86, 674
BstAFI CTTAAG 1 cut(s) 3922
BstAPI GCANNNNNTGC 4 cut(s) 428, 1421, 1945, 3128
BstBAI YACGTR 1 cut(s) 3266
BstBI TTCGAA 1 cut(s) 2287
BstDSI CCRYGG 5 cut(s) 12, 1102, 4598, 5272, 5860
BstEII GGTNACC 1 cut(s) 2298
BstFNI CGCG 2 cut(s) 59, 1032
BstH2I RGCGCY 2 cut(s) 89, 2641
BstHHI GCGC 6 cut(s) 61, 88, 1092, 2640, 3271, 3510
BstMCI CGRYCG 2 cut(s) 543, 2007
BstNSI RCATGY 3 cut(s) 1939, 4460, 4641
BstPAI GACNNNNGTC 1 cut(s) 1797
BstPI GGTNACC 1 cut(s) 2298
BstSLI GKGCMC 2 cut(s) 1114, 5639
BstUI CGCG 2 cut(s) 59, 1032
BstV2I GAAGAC 7 cut(s) 126, 135, 156, 492, 611, 3854, 4800
BstX2I RGATCY 4 cut(s) 1181, 2237, 4066, 5203
BstXI CCANNNNNNTGG 1 cut(s) 5168
BstYI RGATCY 4 cut(s) 1181, 2237, 4066, 5203
Bsu36I CCTNAGG 1 cut(s) 2275
BtgI CCRYGG 5 cut(s) 12, 1102, 4598, 5272, 5860
BtgZI GCGATG 2 cut(s) 1047, 4385
BtrI CACGTC 3 cut(s) 1246, 3461, 5024
BtsI GCAGTG 5 cut(s) 1018, 3013, 3623, 4659, 5839
BveI ACCTGC 5 cut(s) 814, 3113, 3387, 3958, 4243
CaiI CAGNNNCTG 5 cut(s) 428, 829, 1403, 1945, 4387
CfoI GCGC 6 cut(s) 61, 88, 1092, 2640, 3271, 3510
Cfr10I RCCGGY 1 cut(s) 4980
CseI GACGC 2 cut(s) 399, 2351
CsiI ACCWGGT 2 cut(s) 2055, 2064
Csp6I GTAC 8 cut(s) 1227, 2368, 2696, 3024, 3030, 4255, 4626, 5234
CspAI ACCGGT 1 cut(s) 4980
CspCI CAANNNNNGTGG 2 cut(s) 3380, 3415
CviQI GTAC 8 cut(s) 1227, 2368, 2696, 3024, 3030, 4255, 4626, 5234
DinI GGCGCC 1 cut(s) 87
DraI TTTAAA 1 cut(s) 2217
EaeI YGGCCR 4 cut(s) 165, 3606, 3909, 4595
Eam1104I CTCTTC 5 cut(s) 2049, 2409, 3679, 4434, 5574
EarI CTCTTC 5 cut(s) 2049, 2409, 3679, 4434, 5574
EciI GGCGGA 2 cut(s) 60, 4618
Ecl136II GAGCTC 2 cut(s) 2378, 4696
Eco130I CCWWGG 7 cut(s) 12, 46, 168, 854, 3660, 4598, 5860
Eco24I GRGCYC 3 cut(s) 2380, 4698, 5639
Eco31I GGTCTC 2 cut(s) 589, 4128
Eco32I GATATC 4 cut(s) 1363, 3105, 3979, 4118
Eco47I GGWCC 5 cut(s) 1766, 2524, 2537, 2797, 4108
Eco47III AGCGCT 1 cut(s) 2639
Eco53kI GAGCTC 2 cut(s) 2378, 4696
Eco57I CTGAAG 6 cut(s) 623, 1425, 3704, 3848, 4409, 5043
Eco81I CCTNAGG 1 cut(s) 2275
Eco88I CYCGRG 2 cut(s) 2093, 2801
Eco91I GGTNACC 1 cut(s) 2298
EcoICRI GAGCTC 2 cut(s) 2378, 4696
EcoO109I RGGNCCY 2 cut(s) 2623, 5636
EcoO65I GGTNACC 1 cut(s) 2298
EcoRI GAATTC 4 cut(s) 1237, 1493, 2722, 3451
EcoRV GATATC 4 cut(s) 1363, 3105, 3979, 4118
EcoT14I CCWWGG 7 cut(s) 12, 46, 168, 854, 3660, 4598, 5860
EcoT22I ATGCAT 5 cut(s) 2668, 3961, 4643, 4843, 4869
EcoT38I GRGCYC 3 cut(s) 2380, 4698, 5639
EgeI GGCGCC 1 cut(s) 87
EheI GGCGCC 1 cut(s) 87
ErhI CCWWGG 7 cut(s) 12, 46, 168, 854, 3660, 4598, 5860
FalI AAGNNNNNCTT 6 cut(s) 1468, 1500, 2446, 2478, 5221, 5253
FauI CCCGC 1 cut(s) 5094
FauNDI CATATG 2 cut(s) 1880, 4873
FbaI TGATCA 2 cut(s) 5128, 5472
FblI GTMKAC 3 cut(s) 1791, 3262, 4165
FriOI GRGCYC 3 cut(s) 2380, 4698, 5639
GlaI GCGC 6 cut(s) 60, 87, 1091, 2639, 3270, 3509
GsaI CCCAGC 5 cut(s) 219, 2982, 3135, 4185, 5172
GsuI CTGGAG 3 cut(s) 2262, 3170, 3320
HaeII RGCGCY 2 cut(s) 89, 2641
HgaI GACGC 2 cut(s) 399, 2351
HhaI GCGC 6 cut(s) 61, 88, 1092, 2640, 3271, 3510
Hin1I GRCGYC 2 cut(s) 86, 674
Hin6I GCGC 6 cut(s) 59, 86, 1090, 2638, 3269, 3508
HinP1I GCGC 6 cut(s) 59, 86, 1090, 2638, 3269, 3508
HincII GTYRAC 3 cut(s) 672, 1170, 5389
HindII GTYRAC 3 cut(s) 672, 1170, 5389
HindIII AAGCTT 2 cut(s) 1210, 3793
HpaI GTTAAC 1 cut(s) 5389
Hpy99I CGWCG 5 cut(s) 23, 128, 5343, 5612, 5717
HpyCH4IV ACGT 9 cut(s) 489, 674, 943, 1245, 1794, 2959, 3265, 3460, 5023
HpySE526I ACGT 9 cut(s) 489, 674, 943, 1245, 1794, 2959, 3265, 3460, 5023
Hsp92I GRCGYC 2 cut(s) 86, 674
HspAI GCGC 6 cut(s) 59, 86, 1090, 2638, 3269, 3508
KasI GGCGCC 1 cut(s) 85
Kpn2I TCCGGA 3 cut(s) 677, 5208, 5298
KpnI GGTACC 2 cut(s) 2699, 4629
Ksp22I TGATCA 2 cut(s) 5128, 5472
KspAI GTTAAC 1 cut(s) 5389
LguI GCTCTTC 1 cut(s) 2049
MabI ACCWGGT 2 cut(s) 2055, 2064
MaeII ACGT 9 cut(s) 489, 674, 943, 1245, 1794, 2959, 3265, 3460, 5023
MaeIII GTNAC 9 cut(s) 859, 2298, 2514, 2686, 3445, 4446, 4501, 4655, 4759
MfeI CAATTG 2 cut(s) 1575, 3312
MflI RGATCY 4 cut(s) 1181, 2237, 4066, 5203
MhlI GDGCHC 8 cut(s) 1114, 1888, 2044, 2102, 2380, 4011, 4698, 5639
MlsI TGGCCA 3 cut(s) 167, 3608, 3911
MluNI TGGCCA 3 cut(s) 167, 3608, 3911
Mly113I GGCGCC 1 cut(s) 86
MmeI TCCRAC 6 cut(s) 1330, 2589, 3247, 3322, 4227, 4965
Mox20I TGGCCA 3 cut(s) 167, 3608, 3911
Mph1103I ATGCAT 5 cut(s) 2668, 3961, 4643, 4843, 4869
MroI TCCGGA 3 cut(s) 677, 5208, 5298
MroXI GAANNNNTTC 2 cut(s) 3363, 3878
MscI TGGCCA 3 cut(s) 167, 3608, 3911
MslI CAYNNNNRTG 5 cut(s) 846, 1659, 2349, 4785, 4872
Msp20I TGGCCA 3 cut(s) 167, 3608, 3911
MspA1I CMGCKG 5 cut(s) 425, 2990, 3617, 5009, 5327
MspCI CTTAAG 1 cut(s) 3922
MunI CAATTG 2 cut(s) 1575, 3312
Mva1269I GAATGC 4 cut(s) 1291, 1600, 4867, 5769
MvnI CGCG 2 cut(s) 59, 1032
NarI GGCGCC 1 cut(s) 86
NciI CCSGG 4 cut(s) 83, 1179, 4105, 4510
NcoI CCATGG 3 cut(s) 12, 4598, 5860
NdeI CATATG 2 cut(s) 1880, 4873
NheI GCTAGC 2 cut(s) 3464, 5012
NmeAIII GCCGAG 2 cut(s) 3305, 5596
NmuCI GTSAC 3 cut(s) 2298, 3445, 4655
NsiI ATGCAT 5 cut(s) 2668, 3961, 4643, 4843, 4869
NspI RCATGY 3 cut(s) 1939, 4460, 4641
NspV TTCGAA 1 cut(s) 2287
OliI CACNNNNGTG 1 cut(s) 2349
PaeI GCATGC 1 cut(s) 1939
PaeR7I CTCGAG 1 cut(s) 2093
PaqCI CACCTGC 3 cut(s) 3387, 3958, 4243
PciI ACATGT 1 cut(s) 4456
PciSI GCTCTTC 1 cut(s) 2049
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 4 cut(s) 1291, 1600, 4867, 5769
PdmI GAANNNNTTC 2 cut(s) 3363, 3878
PflMI CCANNNNNTGG 1 cut(s) 971
PfoI TCCNGGA 2 cut(s) 1335, 4069
PinAI ACCGGT 1 cut(s) 4980
Ple19I CGATCG 1 cut(s) 2007
PluTI GGCGCC 1 cut(s) 89
Ppu21I YACGTR 1 cut(s) 3266
PscI ACATGT 1 cut(s) 4456
PshAI GACNNNNGTC 1 cut(s) 1797
PshBI ATTAAT 2 cut(s) 371, 738
PsiI TTATAA 1 cut(s) 5186
Psp124BI GAGCTC 2 cut(s) 2380, 4698
Psp1406I AACGTT 1 cut(s) 943
PspEI GGTNACC 1 cut(s) 2298
PspFI CCCAGC 5 cut(s) 215, 2978, 3131, 4181, 5168
PspOMI GGGCCC 1 cut(s) 5635
PsrI GAACNNNNNNTAC 2 cut(s) 4752, 4784
PstI CTGCAG 6 cut(s) 1399, 1525, 2019, 2514, 2569, 3717
PstNI CAGNNNCTG 5 cut(s) 428, 829, 1403, 1945, 4387
PsuI RGATCY 4 cut(s) 1181, 2237, 4066, 5203
PvuI CGATCG 1 cut(s) 2007
PvuII CAGCTG 4 cut(s) 425, 2990, 3617, 5009
RsaI GTAC 8 cut(s) 1228, 2369, 2697, 3025, 3031, 4256, 4627, 5235
RsaNI GTAC 8 cut(s) 1227, 2368, 2696, 3024, 3030, 4255, 4626, 5234
RseI CAYNNNNRTG 5 cut(s) 846, 1659, 2349, 4785, 4872
SacI GAGCTC 2 cut(s) 2380, 4698
SapI GCTCTTC 1 cut(s) 2049
ScaI AGTACT 1 cut(s) 2369
SduI GDGCHC 8 cut(s) 1114, 1888, 2044, 2102, 2380, 4011, 4698, 5639
SexAI ACCWGGT 2 cut(s) 2055, 2064
SfoI GGCGCC 1 cut(s) 87
Sfr274I CTCGAG 1 cut(s) 2093
SfuI TTCGAA 1 cut(s) 2287
SinI GGWCC 5 cut(s) 1766, 2524, 2537, 2797, 4108
SlaI CTCGAG 1 cut(s) 2093
SmiMI CAYNNNNRTG 5 cut(s) 846, 1659, 2349, 4785, 4872
SmlI CTYRAG 5 cut(s) 1084, 1733, 2093, 3922, 5768
SmoI CTYRAG 5 cut(s) 1084, 1733, 2093, 3922, 5768
SphI GCATGC 1 cut(s) 1939
SspDI GGCGCC 1 cut(s) 85
SspI AATATT 1 cut(s) 4147
SstI GAGCTC 2 cut(s) 2380, 4698
StyI CCWWGG 7 cut(s) 12, 46, 168, 854, 3660, 4598, 5860
TaiI ACGT 9 cut(s) 492, 677, 946, 1248, 1797, 2962, 3268, 3463, 5026
TaqII GACCGA 3 cut(s) 529, 2525, 4110
TatI WGTACW 2 cut(s) 1226, 2367
TauI GCSGC 8 cut(s) 11, 59, 1568, 1935, 3143, 3251, 3491, 5366
TseFI GTSAC 3 cut(s) 2298, 3445, 4655
Tsp45I GTSAC 3 cut(s) 2298, 3445, 4655
TspGWI ACGGA 4 cut(s) 403, 704, 3867, 5153
Van91I CCANNNNNTGG 1 cut(s) 971
Vha464I CTTAAG 1 cut(s) 3922
VpaK11BI GGWCC 5 cut(s) 1766, 2524, 2537, 2797, 4108
VspI ATTAAT 2 cut(s) 371, 738
XbaI TCTAGA 1 cut(s) 1118
XceI RCATGY 3 cut(s) 1939, 4460, 4641
XhoI CTCGAG 1 cut(s) 2093
XmiI GTMKAC 3 cut(s) 1791, 3262, 4165
XmnI GAANNNNTTC 2 cut(s) 3363, 3878
ZraI GACGTC 1 cut(s) 675
ZrmI AGTACT 1 cut(s) 2369
Zsp2I ATGCAT 5 cut(s) 2668, 3961, 4643, 4843, 4869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.