Rw6G044640

DDB1- and CUL4-associated factor homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0013230
Physical Location & Seq
Forward (+)
67751861 .. 67761373
9513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G044640.1

Sequence Viewer

Length: 5880 bp
ATGGAGGCCGGCATGGACGACGAGCTACAGACCCAGGGCCAGGGCCAAGGCCAAGCCGCGCCACCAGCTCCGCCGCCTCCCCCGGCGCCGGAATCGCAGGCAGGCGAGGAGCCGGAGGAAGACGACGAAGACGAGGAGCCGAAGAATGAAGACGATGAAATGGTGGCCAAGGCTCAGAAGCTCATGGATAAGATTATTGCTTCGCCTGATAACCCCAGCCCCACTGTTCTTCACGCCCTCGCTTCACTTCTTGAAACGCAGGAGAAACGATACATGGAGGAGAGTGGTCATTCTTCCAGCAATGGTCGTGCTTCTCATAACATTGGACGGTTGGGGAACTTGGTTCGGGAACATGATGACTTCTTTGAATTAATATCTGCCAAGTATTTGTCAGATACGAGATACTCTGCCTCCGTCCAGGCAGCTGCTGCAAGGCTGTTTCTGAGCTGTTCGCTGACTTTGATTTATCCTCATGTTTTTGAAGAAGACGTTTTGGAGAAAATAAAGGACTGGGTGATGGATGAGACTTCCAGTGTCTCGGTCGAATACCAGAACTGGAAGCATGATTTGGGAGGAAAGGAGGTCTCAGATTTTGAAATGCTGAAGACATATTCTACTGGACTTCTTGCTGTTTGTTTGACTGGGGGTCAAGTAGTGGAAGATGTGTTGACGTCCGGATTGTCAGCCAAACTTATGCGTTATCTTCGTGTCCGTGTTTTGGGGGAGTCAAGTATTAGTCAGAAGGATTCTAGTCATTTAACAGAGACTAAAAACACCTCTGGTGTAAGAGGTAGAGATGAAGGTCGGGGTAGGGTTCGGCAAGTTCTGGAAACAACTCATTTTGATGATCCAAGGGTAACAAGTGAGAGATGCTTGGATGACCAGACTGTTGAAGGGAACCAGGACAGAAGTATCTGTAGGCAAGCGTTTGGAGGAGAACGTTGGGTTGATGGTGGAGAACCACCTGATGGAATGGATGAAGGGGTTGAGATCAATGATGTTGATGGCAGTGATTCTCGTGATTTACGTGATGGGAAACAAAAATTTGGTGATTTTGATGAAAATGGGAGAGATGACTCCTCAAGGCGCAGACCTAACCGTGGATGGGCACGATCCAGAGGGAAGGGAAGGGCAAACGAAGGCGGTGTAGAAAATGAGCAGCTGTTGACCTCACCCGGATCTGCTATTCGGTTGGGACAGGGGCGAAGCTTTAGAGACAAGAGTACATCAAAGAATTCAGACGTGAAAAAAATATCAGATTCTAGGAAGTGTTTGGGCAGGAATGCTTCTGATGTTTTGTTTCTGGAAAGAGAGGATAATGATGAGTGCTTCCAGGATTGCACAGTTGGAAGTAAGGATATCTCTGATCTAGTAAAAAAAGCAGTTCGAGCTGCAGAAGCTGAAGCTAGAGCAGCCAATGCACCTGTAGAAGCCATCAAAGCTGCTGGTGATGCTGCTGCGGAAGTTGTCAAAAGTGCAGCTTTAGAGGAATTCACAACTACAAATAACGAAGAAGCTGCAGTTTTGGCTGCTTCAAGAGCGGCATCTACTGTAATTGATGCCGCCAATTCAATTGAAGCTCTAAGTGCGAATGCAGTAAATACCTCTGCTAGTGCTGAACCAATAACTTCAAGCTCTGAACCCCAAAACCATGAAGATGTTGAAGAATACTTTATTCCGAGTGTTGAATCCCTTGCACAGTTGAGAGAAAAATACTGTATTCAATGTCTTGAGACTCTTGGAGAATATGTTGAAGTTCTTGGACCTGTATTGCATGAGAAGGGAGTAGACGTATGTCTTGCCTTGTTGCAACGAAATTCAAGACATAAAGAACTGTCGAAGGTCGCAATGCTTTTGCCTGATGTAATGAAGCTCATATGTGCTCTGGCTGCTCACCGGAAATTTGCTGCATTGTTCGTGGATAGGGGCGGCATGCAGAAACTGCTTGCTGTCCCTAGAGTTGCTCAAACCTACTTTGGCCTTTCTTCTTGCTTATTTACGATCGGTTCGCTGCAGGGTATAATGGAGCGTGTATGTGCTCTTCCCTCAGACCTGGTTTACCAGGTGGTTGAGTTGGCTCTCCATCTTCTCGAGTGCTCACAGGATCAAGCCAGGAAAAATGCAGCCTTATTTTTTTCTGCTGCATTTGTTTTCAAGGCAGTTCTTGATGCTTTTGATGCTCAGGATGGCTTACAGAAAATACTGGGCCTTTTAAATGATGCTGCATCAGTAAGATCTGGAGTAAATTCTGGGACATTAGGACTCTCGACATCAGGCTCACTTCGAAATGATAGGTCACCGACAGAAGTTCTGACTTCATCAGAGAAACAGATCGCTTATCACACCTGTGTTGCTTTGCGTCAGTACTTTAGAGCTCATTTGATTTTGCTTGTGGATTCTCTTCGTCCAAATAAAAACAGTCGAAGTGCGGCTCGAAATCTTCCTAGTGTAAGGGCAGCTTACAAGCCACTTGACCTTAGCAACGAAGCTATTGATGCAGTATTTCTGCAGTTACAGAAGGACCGGAAACTCGGTCCTGCATTTGTAAGAACTCGTTGGCCTGCAGTTGATAGGTTTTTGGGATATAATGGACATATTACTATGTTGGAGTTATGCCAGGCCCCACCTGTTGAGCGCTATCTACATGATTTACTTCAATATGCATTGGGTGTTCTGCACATTGTTACATTGGTACCTAGCAGTCGCAAGATGATTGTGAATTCAACATTAAGCAACAATCGTGTTGGTATAGCAGTTATATTGGATGCAGCAAGTGTTAATGGTAGTTATGTGGACCCCGAGATCATTCAGCCAGCTCTAAATGTGTTGGTCAATCTCGTTTGTCCTCCACCTTCAATCAGCAACAAACCACCTTTACTTGCACAAAGTCAGCAGTCTGTTTCTGCCCCAACCTCAAATGCCCTTGCTATAGAGACTAGAGAGAAAAATACTGAACGTAATGTCTCAGATCGAGCTGGGGAACCAGCTGTTGTAGATCGAGGCAGTGTAGCAGCACTGGGTACTGGTACACAGTCAAACAGTAACAATGCACTATCCTCTACCCCCACAGCAACTTCTGGATTGGTGGGGGATCGCAGGATATCTTTAGGAGTTGGGGCAGGTTGTGCTGGGCTTGCGGCACAACTGGAGCAAGGCTATCGGCAAGCAAGAGAGGCTGTTCGCTCCACCAACGGTATAAAGGTTCTGCTGCATCTTCTCCAACCACGCATATATTCACCTCCTGCCGCACTCGATTGTCTACGTGCGCTTGCCTGCCGAGTCCTTCTTGGTTTAGCGAGAGATGATACAATTGCACACATATTGACAAAGCTCCAGGTTGGAAAGAAACTATCAGAACTAATTCGAGATCAAACACAAGGAGCTGAGCAAGGCAGGTGGCAATCTGAACTTTCACAGGCAGCAATCGAGTTAATGGCGATTGTGACGAATTCTGGACGTGCTAGCACATTGGCTGCCACTGATGCCGCTATGCCAACTTTGAGGCGCATAGAACGGGCAGCTATAGCTGCTGCCACTCCTATCACTTACCATTCCAGGGAGCTTTTGCTTCTAATACATGAACACCTACAGGCATCTGGTTTGGCCACAACAGCTGCTTCACTGCTAAGAGAGGCCCAGTTGATGCCTCTGCCATCCTTGGCTGCCCCCTCATCTCTTGTTCATCAAGCTACACAAGAAGCCTCATCTCTGCAGCTTCAGTGGCCCTCTGGTCGCACCCCTATTGGATTTCTTACCAACAAATCAAAGATAGCAAGGGAGGAGGATTCAAGCTTGAAATGTGATTCTTCTGTCTCATACTCAAAGAAAAAACCTTTGGTTTTCTCTCCAAGTTTTTGTCTTCAGTCAAGGAATCCGTTCCAGTCCCATGACTCTCACCCAACATTGGCCACAAATGTCTTAAGTGCATCGAAAGAGTTATCCGCACCTGCAAATTCATCAGAAGCTCCATCAGATATCCTGCCGAAACCTAGTATTGATACAGAGTACCAGTGTAAGACACCCATCCTGTTGCCGATGAAAAGAAAGTTATCCGAGTTAAAGGATCCTGGATGTTTGCCATCATCTGGGAAACGCATCCACACCGGGGACCAGGGATATCGGTCTCCAATTTTTCCCACACCCAATATTGTGCGGAAAAGCAGTCTACTAACTGATCTGGCTGGGTTCTCTAGTCCTACTTCCAACATGAGAGATCAACATTGTCGATCCACACCTGCTTGTTTTTCATCAGAGTACCTGGATGATAACCAGTATGGTAATTCTAGTGTAGGTCTGGCAACACCATCCTCCCAACTTGGGCTTCAAAACGACCCTCAACCAAGCAACCCAGAAAGATTGACTCTGGACTCTTTGGTTGTTCAGTATCTGAAGCACCAGCATCGCCAGTGTCCTGCCCCTATAACCACTCTTCCACCACTGTCCCTGTTACAGCCACATGTTTGTCCCGAACCAAGACGAACCCTTGATGCCCCTTCAAATGTAACTGCCCGGCTTGGCACCCGTGAGTTCAGAAGTATGTATGGTGGGGTGCATGGAAACCGCAGGGATCGTCAGTTTGTTTACAGTAGATTCCGGCCATGGCGGACTTGTAGGGATGGTACTGGTACCCCTTTGACATGCATCTCTTTTCTCAGTGACACTGCTCGTATTGCAGTAGGCAGTCATGGTGGAGAGCTCAAAATTTTTGATTCCAATAGCAGCAATCTATTGGAGAGTTGTCCAAGCCACCAGTCTCCTGTAACGCTTGTTCAGACATATCATTCAGGTGAAACCGAATTGGTGCTGTCTTCTAGTTCAGAAGATGTGAGGTTGTGGGATGCATCTACAGTCTCATCTGGGCCAATGCATTCATATGAAGGGTGCAAGGCTGCAAGATTTAGCAATTCTGGGGATGTTTTTGCAGCCCTATCATCAGAGCTGGCGCAAAAAGAAATTCTAATATATGACATCCAAACTAACCGGTTGGAATCAAAACTGTCAGATACAGCTTCTAGCACGGGACGTGGACATTCATATTCTCATATACACTTCAACCCTTCAGACACAATGCTGCTCTGGAATGGTGTCCTATGGGACAGGCGGGTTTCCAGCCCTGTTCATCGTTTTGATCAGTTTACGGATTATGGGGGTGGTGGCTTCCATCCTGCTGGGAACGAGGTGATTATAAACTCTGAGGTCTGGGATCTCCGGAACTTTAGACTCCTCCGAAGTGTACCTTCTTTAGACCAAACTACAATAACTTTTAATGCCCGTGGTGATGTAATTTATGCAATCCTCCGGAGAAATCTTGATGATGTAATGTCGGCGGTGAATACCCGTCGCGTAAAGCATCCTCTCTTTGCTGCTTTCCGCACGGTGGATGCGGTTAACTACTCGGACATTGCCACGATTCCGGTTGATCGTTGTGTCCTCGACTTTGCAACAGAGCCAACTGATTCATTTCTTGGGCTGATCACAATGGATGACCAAGATGAGATGTTTGCTTCTGCTAGGGTTTATGAAATTGGTCGCCGAAAGCCGACAGATGATGATTCTGATCCTGATGATGCCGAGAGTGAAGACGACGACGATGATGAAGAAGACGACGACGATGCGGATATGGACACTTTACTAGGCCCTGATCTTGACGGGGATGATGATAGTGATATGAGTAATGATGATGATGATGATGATGATGGGAGCGACCTTGATGAGGACGATGAGGGAGATTATATGATGGATGGGGACTTTGATGGAGGAGGTGGAATGCTTGAGATTTTGACAGACGGTGAGGAAGATGACGATGACAGTGATGGACTGGTTGAATCCTTTAGCAGTGGTGATGAGGAAGATTTTGCAGGCCATGGTTTTGGTTATTAA

Protein Analysis

1959

Amino Acids

213.41

Weight (kDa)

4.94

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 1542 - 1616 1.7e-06 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 1543 - 1618 9.8e-07 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 1544 - 1669 3.3e-08 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 1546 - 1691 1.5e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013230)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31160
fragaria_vesca FvH4_2g30560
malus_domestica MD08G1132100.v1.1 MD15G1113300.v1.1
prunus_persica Prupe.1G463700_v2.0.a1
pyrus_communis pycom08g11060 pycom15g10160
rosa_chinensis RchiOBHm_Chr6g0312521
rosa_laevigata RLG00000010322
rosa_multiflora Rmu_sc0003094.1_g000015 Rmu_sc0035272.1_g000001
rosa_roxburghii Rroxscaffold_7G00156780
rosa_rugosa Rorug06G0396000
rosa_samantha Rh6AG513400 Rh6BG526600 Rh6CG531500 Rh6DG515500
rosa_wichuraiana Rw6G044640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 5186
AarI CACCTGC 3 cut(s) 3384, 3955, 4243
AatII GACGTC 1 cut(s) 674
Acc36I ACCTGC 4 cut(s) 3110, 3384, 3955, 4243
Acc65I GGTACC 2 cut(s) 2692, 4625
AccB1I GGYRCC 4 cut(s) 85, 2692, 4517, 4625
AccB7I CCANNNNNTGG 2 cut(s) 968, 4085
AccBSI CCGCTC 1 cut(s) 1541
AccI GTMKAC 3 cut(s) 1788, 3259, 4165
AccII CGCG 2 cut(s) 59, 5343
AccIII TCCGGA 3 cut(s) 674, 5208, 5298
AclI AACGTT 1 cut(s) 940
AcoI YGGCCR 4 cut(s) 165, 3603, 3906, 4595
AcuI CTGAAG 6 cut(s) 623, 1422, 3701, 3845, 4409, 5043
AcyI GRCGYC 2 cut(s) 86, 671
AfeI AGCGCT 1 cut(s) 2636
AflII CTTAAG 1 cut(s) 3919
AflIII ACRYGT 1 cut(s) 4456
AgeI ACCGGT 1 cut(s) 4980
AhdI GACNNNNNGTC 1 cut(s) 645
AjiI CACGTC 3 cut(s) 1243, 3458, 5024
AjuI GAANNNNNNNTTGG 6 cut(s) 551, 583, 3818, 3850, 4305, 4337
AleI CACNNNNGTG 1 cut(s) 2346
Alw21I GWGCWC 5 cut(s) 1885, 2041, 2099, 2377, 4698
AlwNI CAGNNNCTG 4 cut(s) 428, 1400, 1942, 4387
Ama87I CYCGRG 2 cut(s) 2090, 2798
Aor13HI TCCGGA 3 cut(s) 674, 5208, 5298
Aor51HI AGCGCT 1 cut(s) 2636
AseI ATTAAT 1 cut(s) 371
AsiGI ACCGGT 1 cut(s) 4980
Asp700I GAANNNNTTC 2 cut(s) 3360, 3875
Asp718I GGTACC 2 cut(s) 2692, 4625
AspLEI GCGC 7 cut(s) 61, 88, 1089, 2637, 3268, 3507, 4945
AsuC2I CCSGG 4 cut(s) 83, 1176, 4105, 4510
AsuII TTCGAA 1 cut(s) 2284
AsuNHI GCTAGC 1 cut(s) 3461
AvaI CYCGRG 2 cut(s) 2090, 2798
AvaII GGWCC 5 cut(s) 1763, 2521, 2534, 2794, 4108
BaeGI GKGCMC 1 cut(s) 1111
BaeI ACNNNNGTAYC 4 cut(s) 895, 928, 2676, 2709
BalI TGGCCA 3 cut(s) 167, 3605, 3908
BamHI GGATCC 1 cut(s) 4063
BanI GGYRCC 4 cut(s) 85, 2692, 4517, 4625
BanII GRGCYC 2 cut(s) 2377, 4698
BarI GAAGNNNNNNTAC 2 cut(s) 2025, 2057
BauI CACGAG 1 cut(s) 1017
BbsI GAAGAC 9 cut(s) 126, 135, 156, 492, 611, 3851, 4800, 5586, 5607
Bbv12I GWGCWC 5 cut(s) 1885, 2041, 2099, 2377, 4698
BcgI CGANNNNNNTGC 8 cut(s) 1499, 1533, 3140, 3174, 4382, 4416, 5594, 5628
BclI TGATCA 2 cut(s) 5128, 5472
BcnI CCSGG 4 cut(s) 83, 1176, 4105, 4510
BfoI RGCGCY 2 cut(s) 89, 2638
BfrI CTTAAG 1 cut(s) 3919
BfuAI ACCTGC 4 cut(s) 3110, 3384, 3955, 4243
BglII AGATCT 1 cut(s) 2234
BlpI GCTNAGC 1 cut(s) 3384
BmcAI AGTACT 1 cut(s) 2366
Bme18I GGWCC 5 cut(s) 1763, 2521, 2534, 2794, 4108
BmeRI GACNNNNNGTC 1 cut(s) 645
BmeT110I CYCGRG 2 cut(s) 2090, 2798
BmgBI CACGTC 3 cut(s) 1243, 3458, 5024
BmrI ACTGGG 5 cut(s) 520, 651, 2213, 3026, 3631
BmtI GCTAGC 1 cut(s) 3465
BmuI ACTGGG 5 cut(s) 520, 651, 2213, 3026, 3631
BoxI GACNNNNGTC 1 cut(s) 1794
BpiI GAAGAC 9 cut(s) 126, 135, 156, 492, 611, 3851, 4800, 5586, 5607
BpmI CTGGAG 3 cut(s) 2259, 3167, 3317
Bpu10I CCTNAGC 2 cut(s) 2181, 2477
Bpu1102I GCTNAGC 1 cut(s) 3384
Bpu14I TTCGAA 1 cut(s) 2284
BpuEI CTTGAG 3 cut(s) 1066, 1751, 5792
BpuMI CCSGG 4 cut(s) 83, 1176, 4105, 4510
BsaAI YACGTR 2 cut(s) 1028, 3263
BsaBI GATNNNNATC 1 cut(s) 5556
BsaHI GRCGYC 2 cut(s) 86, 671
BsaI GGTCTC 2 cut(s) 589, 4128
BsaWI WCCGGW 7 cut(s) 674, 1896, 2523, 4980, 5208, 5298, 5413
BsaXI ACNNNNNCTCC 6 cut(s) 395, 425, 1734, 1764, 1776, 1806
Bse118I RCCGGY 2 cut(s) 8, 4980
Bse3DI GCAATG 3 cut(s) 307, 1854, 5400
Bse8I GATNNNNATC 1 cut(s) 5556
BseAI TCCGGA 3 cut(s) 674, 5208, 5298
BseJI GATNNNNATC 1 cut(s) 5556
BseMI GCAATG 3 cut(s) 307, 1854, 5400
BseMII CTCAG 9 cut(s) 188, 434, 600, 2061, 2195, 2979, 3375, 4666, 5184
BseRI GAGGAG 8 cut(s) 122, 149, 293, 948, 1069, 3794, 5213, 5772
BseSI GKGCMC 1 cut(s) 1111
BseYI CCCAGC 5 cut(s) 215, 2975, 3128, 4181, 5168
BsgI GTGCAG 2 cut(s) 1497, 2660
Bsh1236I CGCG 2 cut(s) 59, 5343
Bsh1285I CGRYCG 2 cut(s) 543, 2004
BshNI GGYRCC 4 cut(s) 85, 2692, 4517, 4625
BshTI ACCGGT 1 cut(s) 4980
BsiEI CGRYCG 2 cut(s) 543, 2004
BsiHKAI GWGCWC 5 cut(s) 1885, 2041, 2099, 2377, 4698
BsiHKCI CYCGRG 2 cut(s) 2090, 2798
BsmI GAATGC 4 cut(s) 1288, 1597, 4867, 5772
Bso31I GGTCTC 2 cut(s) 589, 4128
BsoBI CYCGRG 2 cut(s) 2090, 2798
Bsp119I TTCGAA 1 cut(s) 2284
Bsp1286I GDGCHC 6 cut(s) 1111, 1885, 2041, 2099, 2377, 4698
Bsp13I TCCGGA 3 cut(s) 674, 5208, 5298
Bsp1720I GCTNAGC 1 cut(s) 3384
Bsp19I CCATGG 2 cut(s) 4598, 5863
BspCNI CTCAG 9 cut(s) 187, 435, 599, 2060, 2194, 2978, 3376, 4665, 5185
BspEI TCCGGA 3 cut(s) 674, 5208, 5298
BspFNI CGCG 2 cut(s) 59, 5343
BspMAI CTGCAG 6 cut(s) 1396, 1522, 2016, 2511, 2566, 3714
BspMI ACCTGC 4 cut(s) 3110, 3384, 3955, 4243
BspOI GCTAGC 1 cut(s) 3465
BspQI GCTCTTC 1 cut(s) 2046
BspT104I TTCGAA 1 cut(s) 2284
BspT107I GGYRCC 4 cut(s) 85, 2692, 4517, 4625
BspTI CTTAAG 1 cut(s) 3919
BspTNI GGTCTC 2 cut(s) 589, 4128
BsrBI CCGCTC 1 cut(s) 1541
BsrDI GCAATG 3 cut(s) 307, 1854, 5400
BsrFI RCCGGY 2 cut(s) 8, 4980
BssAI RCCGGY 2 cut(s) 8, 4980
BssNI GRCGYC 2 cut(s) 86, 671
BssSI CACGAG 1 cut(s) 1017
BssT1I CCWWGG 6 cut(s) 46, 168, 851, 3657, 4598, 5863
Bst2BI CACGAG 1 cut(s) 1017
Bst6I CTCTTC 3 cut(s) 2046, 2406, 4434
BstACI GRCGYC 2 cut(s) 86, 671
BstAFI CTTAAG 1 cut(s) 3919
BstAPI GCANNNNNTGC 4 cut(s) 428, 1418, 1942, 3125
BstBAI YACGTR 2 cut(s) 1028, 3263
BstBI TTCGAA 1 cut(s) 2284
BstDSI CCRYGG 4 cut(s) 1099, 4598, 5272, 5863
BstEII GGTNACC 1 cut(s) 2295
BstENI CCTNNNNNAGG 1 cut(s) 5711
BstFNI CGCG 2 cut(s) 59, 5343
BstH2I RGCGCY 2 cut(s) 89, 2638
BstHHI GCGC 7 cut(s) 61, 88, 1089, 2637, 3268, 3507, 4945
BstMCI CGRYCG 2 cut(s) 543, 2004
BstNSI RCATGY 3 cut(s) 1936, 4460, 4641
BstPAI GACNNNNGTC 1 cut(s) 1794
BstPI GGTNACC 1 cut(s) 2295
BstSLI GKGCMC 1 cut(s) 1111
BstUI CGCG 2 cut(s) 59, 5343
BstV2I GAAGAC 9 cut(s) 126, 135, 156, 492, 611, 3851, 4800, 5586, 5607
BstX2I RGATCY 4 cut(s) 1178, 2234, 4063, 5203
BstXI CCANNNNNNTGG 2 cut(s) 5168, 5870
BstYI RGATCY 4 cut(s) 1178, 2234, 4063, 5203
BtgI CCRYGG 4 cut(s) 1099, 4598, 5272, 5863
BtgZI GCGATG 1 cut(s) 4385
BtrI CACGTC 3 cut(s) 1243, 3458, 5024
BtsI GCAGTG 5 cut(s) 1015, 3010, 3620, 4659, 5842
BveI ACCTGC 4 cut(s) 3110, 3384, 3955, 4243
CaiI CAGNNNCTG 4 cut(s) 428, 1400, 1942, 4387
CfoI GCGC 7 cut(s) 61, 88, 1089, 2637, 3268, 3507, 4945
Cfr10I RCCGGY 2 cut(s) 8, 4980
CseI GACGC 1 cut(s) 2348
CsiI ACCWGGT 2 cut(s) 2052, 2061
CspAI ACCGGT 1 cut(s) 4980
CspCI CAANNNNNGTGG 2 cut(s) 3377, 3412
DinI GGCGCC 1 cut(s) 87
DraI TTTAAA 1 cut(s) 2214
DriI GACNNNNNGTC 1 cut(s) 645
EaeI YGGCCR 4 cut(s) 165, 3603, 3906, 4595
Eam1104I CTCTTC 3 cut(s) 2046, 2406, 4434
Eam1105I GACNNNNNGTC 1 cut(s) 645
EarI CTCTTC 3 cut(s) 2046, 2406, 4434
EciI GGCGGA 2 cut(s) 60, 4618
Ecl136II GAGCTC 2 cut(s) 2375, 4696
Eco130I CCWWGG 6 cut(s) 46, 168, 851, 3657, 4598, 5863
Eco24I GRGCYC 2 cut(s) 2377, 4698
Eco31I GGTCTC 2 cut(s) 589, 4128
Eco32I GATATC 4 cut(s) 1360, 3102, 3976, 4118
Eco47I GGWCC 5 cut(s) 1763, 2521, 2534, 2794, 4108
Eco47III AGCGCT 1 cut(s) 2636
Eco53kI GAGCTC 2 cut(s) 2375, 4696
Eco57I CTGAAG 6 cut(s) 623, 1422, 3701, 3845, 4409, 5043
Eco88I CYCGRG 2 cut(s) 2090, 2798
Eco91I GGTNACC 1 cut(s) 2295
EcoICRI GAGCTC 2 cut(s) 2375, 4696
EcoNI CCTNNNNNAGG 1 cut(s) 5711
EcoO109I RGGNCCY 2 cut(s) 2620, 5636
EcoO65I GGTNACC 1 cut(s) 2295
EcoRI GAATTC 4 cut(s) 1234, 1490, 2719, 3448
EcoRV GATATC 4 cut(s) 1360, 3102, 3976, 4118
EcoT14I CCWWGG 6 cut(s) 46, 168, 851, 3657, 4598, 5863
EcoT22I ATGCAT 4 cut(s) 2665, 4643, 4843, 4869
EcoT38I GRGCYC 2 cut(s) 2377, 4698
EgeI GGCGCC 1 cut(s) 87
EheI GGCGCC 1 cut(s) 87
ErhI CCWWGG 6 cut(s) 46, 168, 851, 3657, 4598, 5863
FalI AAGNNNNNCTT 6 cut(s) 1465, 1497, 2443, 2475, 5221, 5253
FauI CCCGC 1 cut(s) 5094
FauNDI CATATG 2 cut(s) 1877, 4873
FbaI TGATCA 2 cut(s) 5128, 5472
FblI GTMKAC 3 cut(s) 1788, 3259, 4165
FriOI GRGCYC 2 cut(s) 2377, 4698
GlaI GCGC 7 cut(s) 60, 87, 1088, 2636, 3267, 3506, 4944
GsaI CCCAGC 5 cut(s) 219, 2979, 3132, 4185, 5172
GsuI CTGGAG 3 cut(s) 2259, 3167, 3317
HaeII RGCGCY 2 cut(s) 89, 2638
HgaI GACGC 1 cut(s) 2348
HhaI GCGC 7 cut(s) 61, 88, 1089, 2637, 3268, 3507, 4945
Hin1I GRCGYC 2 cut(s) 86, 671
Hin6I GCGC 7 cut(s) 59, 86, 1087, 2635, 3266, 3505, 4943
HinP1I GCGC 7 cut(s) 59, 86, 1087, 2635, 3266, 3505, 4943
HincII GTYRAC 3 cut(s) 669, 1167, 5389
HindII GTYRAC 3 cut(s) 669, 1167, 5389
HindIII AAGCTT 2 cut(s) 1207, 3790
HpaI GTTAAC 1 cut(s) 5389
Hpy99I CGWCG 7 cut(s) 23, 128, 5343, 5588, 5591, 5609, 5612
Hsp92I GRCGYC 2 cut(s) 86, 671
HspAI GCGC 7 cut(s) 59, 86, 1087, 2635, 3266, 3505, 4943
KasI GGCGCC 1 cut(s) 85
Kpn2I TCCGGA 3 cut(s) 674, 5208, 5298
KpnI GGTACC 2 cut(s) 2696, 4629
KroI GCCGGC 1 cut(s) 8
KroNI GCCGGC 1 cut(s) 10
Ksp22I TGATCA 2 cut(s) 5128, 5472
KspAI GTTAAC 1 cut(s) 5389
LguI GCTCTTC 1 cut(s) 2046
MabI ACCWGGT 2 cut(s) 2052, 2061
MbiI CCGCTC 1 cut(s) 1541
MfeI CAATTG 2 cut(s) 1572, 3309
MflI RGATCY 4 cut(s) 1178, 2234, 4063, 5203
MhlI GDGCHC 6 cut(s) 1111, 1885, 2041, 2099, 2377, 4698
MlsI TGGCCA 3 cut(s) 167, 3605, 3908
MluNI TGGCCA 3 cut(s) 167, 3605, 3908
Mly113I GGCGCC 1 cut(s) 86
MlyI GAGTC 9 cut(s) 734, 1070, 1729, 2256, 3288, 3884, 4354, 4361, 5214
MmeI TCCRAC 6 cut(s) 1327, 2586, 3244, 3319, 4227, 4965
Mox20I TGGCCA 3 cut(s) 167, 3605, 3908
Mph1103I ATGCAT 4 cut(s) 2665, 4643, 4843, 4869
MroI TCCGGA 3 cut(s) 674, 5208, 5298
MroNI GCCGGC 1 cut(s) 8
MroXI GAANNNNTTC 2 cut(s) 3360, 3875
MscI TGGCCA 3 cut(s) 167, 3605, 3908
MslI CAYNNNNRTG 5 cut(s) 843, 1656, 2346, 4785, 4872
Msp20I TGGCCA 3 cut(s) 167, 3605, 3908
MspA1I CMGCKG 4 cut(s) 425, 1162, 2987, 3614
MspCI CTTAAG 1 cut(s) 3919
MunI CAATTG 2 cut(s) 1572, 3309
Mva1269I GAATGC 4 cut(s) 1288, 1597, 4867, 5772
MvnI CGCG 2 cut(s) 59, 5343
NaeI GCCGGC 1 cut(s) 10
NarI GGCGCC 1 cut(s) 86
NciI CCSGG 4 cut(s) 83, 1176, 4105, 4510
NcoI CCATGG 2 cut(s) 4598, 5863
NdeI CATATG 2 cut(s) 1877, 4873
NgoMIV GCCGGC 1 cut(s) 8
NheI GCTAGC 1 cut(s) 3461
NmeAIII GCCGAG 2 cut(s) 3302, 5596
NmuCI GTSAC 3 cut(s) 2295, 3442, 4655
NsiI ATGCAT 4 cut(s) 2665, 4643, 4843, 4869
NspI RCATGY 3 cut(s) 1936, 4460, 4641
NspV TTCGAA 1 cut(s) 2284
OliI CACNNNNGTG 1 cut(s) 2346
PaeI GCATGC 1 cut(s) 1936
PaeR7I CTCGAG 1 cut(s) 2090
PaqCI CACCTGC 3 cut(s) 3384, 3955, 4243
PasI CCCWGGG 1 cut(s) 34
PciI ACATGT 1 cut(s) 4456
PciSI GCTCTTC 1 cut(s) 2046
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 1024
PctI GAATGC 4 cut(s) 1288, 1597, 4867, 5772
PdiI GCCGGC 1 cut(s) 10
PdmI GAANNNNTTC 2 cut(s) 3360, 3875
PflMI CCANNNNNTGG 2 cut(s) 968, 4085
PfoI TCCNGGA 2 cut(s) 1332, 4066
PinAI ACCGGT 1 cut(s) 4980
Ple19I CGATCG 1 cut(s) 2004
PleI GAGTC 9 cut(s) 733, 1070, 1729, 2256, 3287, 3884, 4354, 4361, 5214
PluTI GGCGCC 1 cut(s) 89
PpsI GAGTC 9 cut(s) 733, 1070, 1729, 2256, 3287, 3884, 4354, 4361, 5214
Ppu21I YACGTR 2 cut(s) 1028, 3263
PscI ACATGT 1 cut(s) 4456
PshAI GACNNNNGTC 1 cut(s) 1794
PshBI ATTAAT 1 cut(s) 371
PsiI TTATAA 1 cut(s) 5186
Psp124BI GAGCTC 2 cut(s) 2377, 4698
Psp1406I AACGTT 1 cut(s) 940
PspEI GGTNACC 1 cut(s) 2295
PspFI CCCAGC 5 cut(s) 215, 2975, 3128, 4181, 5168
PsrI GAACNNNNNNTAC 2 cut(s) 4752, 4784
PstI CTGCAG 6 cut(s) 1396, 1522, 2016, 2511, 2566, 3714
PstNI CAGNNNCTG 4 cut(s) 428, 1400, 1942, 4387
PsuI RGATCY 4 cut(s) 1178, 2234, 4063, 5203
PvuI CGATCG 1 cut(s) 2004
PvuII CAGCTG 4 cut(s) 425, 1162, 2987, 3614
RseI CAYNNNNRTG 5 cut(s) 843, 1656, 2346, 4785, 4872
SacI GAGCTC 2 cut(s) 2377, 4698
SapI GCTCTTC 1 cut(s) 2046
ScaI AGTACT 1 cut(s) 2366
SchI GAGTC 9 cut(s) 734, 1070, 1729, 2256, 3288, 3884, 4354, 4361, 5214
SduI GDGCHC 6 cut(s) 1111, 1885, 2041, 2099, 2377, 4698
SexAI ACCWGGT 2 cut(s) 2052, 2061
SfoI GGCGCC 1 cut(s) 87
Sfr274I CTCGAG 1 cut(s) 2090
SfuI TTCGAA 1 cut(s) 2284
SinI GGWCC 5 cut(s) 1763, 2521, 2534, 2794, 4108
SlaI CTCGAG 1 cut(s) 2090
SmiMI CAYNNNNRTG 5 cut(s) 843, 1656, 2346, 4785, 4872
SmlI CTYRAG 5 cut(s) 1081, 1730, 2090, 3919, 5771
SmoI CTYRAG 5 cut(s) 1081, 1730, 2090, 3919, 5771
SphI GCATGC 1 cut(s) 1936
SspDI GGCGCC 1 cut(s) 85
SspI AATATT 1 cut(s) 4147
SstI GAGCTC 2 cut(s) 2377, 4698
StyI CCWWGG 6 cut(s) 46, 168, 851, 3657, 4598, 5863
TaqII GACCGA 3 cut(s) 529, 2522, 4110
TatI WGTACW 2 cut(s) 1223, 2364
TauI GCSGC 9 cut(s) 59, 76, 1544, 1565, 1932, 2432, 3140, 3248, 3488
TseFI GTSAC 3 cut(s) 2295, 3442, 4655
Tsp45I GTSAC 3 cut(s) 2295, 3442, 4655
TspGWI ACGGA 4 cut(s) 403, 701, 3864, 5153
Van91I CCANNNNNTGG 2 cut(s) 968, 4085
Vha464I CTTAAG 1 cut(s) 3919
VpaK11BI GGWCC 5 cut(s) 1763, 2521, 2534, 2794, 4108
VspI ATTAAT 1 cut(s) 371
XagI CCTNNNNNAGG 1 cut(s) 5711
XceI RCATGY 3 cut(s) 1936, 4460, 4641
XhoI CTCGAG 1 cut(s) 2090
XmiI GTMKAC 3 cut(s) 1788, 3259, 4165
XmnI GAANNNNTTC 2 cut(s) 3360, 3875
ZraI GACGTC 1 cut(s) 672
ZrmI AGTACT 1 cut(s) 2366
Zsp2I ATGCAT 4 cut(s) 2665, 4643, 4843, 4869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.