RLG00000011724
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016267
Physical Location & Seq
Forward (+)
12206769 .. 12207767
999 bp
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UTR
Exon/CDS
Intron
RLM00000011724

Sequence Viewer

Length: 804 bp
ATGGCGGTGGCGACAATCGGAGCGGCGTCCCTCGCTCCGATCTTCCAAGAGCTCTGTGATCAAGTCACCCAATCCATGGAGAAGAACACAAAGCTCAAAACCCTCCTGGGATTTTTCCAGTACACGCTCAATTCCTTCAGAGACCGAATCATCCAGCCCGTTCAAGAAGGCCAGCAGATTTTCCACGATCAAGACGACAAGATCGAGTCCGTCGTCGTCGAATTGCAGAAGGGAGCGAAGCTAGTCGCGAAGGCCGTGGAGGCCGAAACGGTGCCGTTGAGGACAGAGACGGCGGAGGCGGCGGTGGAGGAGGAGAGGGGGAGTGGTGAAGAGGAAGAGGCGGCGCTGGTTGTGTTTCGGAGGTTGTTTGAGGCGGTGAGAGAGGCGGAGGAGAAGAACGAGGCGCCCAGGATGTTTAGGTACCTGGTTTCGGATATAGAGAGCACATTGTACTCGTTGCAGCCGTTTTTGGAGGAGATGGCGGCCCACAACAAGGTGTTGGACCGGCCCAAGGAGGAGGTGAGGAGTTTCAGAGTGGAGATGGAGAAGGGGGTGGAGGTGGTTCGGAGGAGCGGTGGGGTTGGTAAGAAGTGGGAGGGATACAGCAAGAAGAAATATGAGTGTATTGATGGGCTGTTGGGGCTGAATCAGTGTCTGCAGAGACAGGTGTGTATTCTGAGATGGCAGATGGCCAAGAATGCGATCCAGGCATTGGATTTGGTGAGGAAAATGGAGAGGGCGGTGAATGAGATCGAAAGCAATGTGGGAGTGCTGGAGCAAGCTGGAGTTGCTAGCACAAGTTGA

Protein Analysis

268

Amino Acids

30.13

Weight (kDa)

5.17

Isoelectric Point (pI)

54.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 118 - 232 6.7e-12 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 420
AccB1I GGYRCC 3 cut(s) 271, 403, 420
AccB7I CCANNNNNTGG 2 cut(s) 76, 712
AccBSI CCGCTC 2 cut(s) 23, 573
AccII CGCG 1 cut(s) 248
AclWI GGATC 1 cut(s) 697
AcoI YGGCCR 1 cut(s) 690
AcuI CTGAAG 1 cut(s) 121
AcyI GRCGYC 2 cut(s) 26, 404
AfaI GTAC 3 cut(s) 122, 422, 452
AfiI CCNNNNNNNGG 5 cut(s) 76, 430, 493, 511, 712
AgsI TTSAA 1 cut(s) 164
AjnI CCWGG 4 cut(s) 105, 407, 423, 705
AluBI AGCT 4 cut(s) 52, 94, 241, 782
AluI AGCT 4 cut(s) 52, 94, 241, 782
Alw21I GWGCWC 2 cut(s) 54, 446
Alw26I GTCTC 3 cut(s) 135, 281, 655
AlwI GGATC 1 cut(s) 697
AlwNI CAGNNNCTG 1 cut(s) 655
AoxI GGCC 6 cut(s) 169, 252, 261, 483, 506, 690
ApeKI GCWGC 1 cut(s) 460
Asp718I GGTACC 1 cut(s) 420
AspLEI GCGC 2 cut(s) 346, 406
AspS9I GGNCC 3 cut(s) 484, 502, 507
AsuHPI GGTGA 6 cut(s) 58, 338, 388, 532, 733, 754
AsuNHI GCTAGC 1 cut(s) 791
AvaII GGWCC 1 cut(s) 502
BalI TGGCCA 1 cut(s) 692
BanI GGYRCC 3 cut(s) 271, 403, 420
BanII GRGCYC 1 cut(s) 54
Bbv12I GWGCWC 2 cut(s) 54, 446
BbvI GCAGC 1 cut(s) 472
BccI CCATC 5 cut(s) 472, 535, 623, 675, 682
BceAI ACGGC 4 cut(s) 239, 259, 306, 448
BciT130I CCWGG 4 cut(s) 107, 409, 425, 707
BciVI GTATCC 1 cut(s) 593
BclI TGATCA 1 cut(s) 58
BcoDI GTCTC 3 cut(s) 135, 281, 655
BfaI CTAG 2 cut(s) 242, 792
BfmI CTRYAG 1 cut(s) 656
BfoI RGCGCY 2 cut(s) 347, 407
BfuI GTATCC 1 cut(s) 593
BglI GCCNNNNNGGC 1 cut(s) 260
BisI GCNGC 5 cut(s) 24, 300, 342, 461, 483
BlsI GCNGC 5 cut(s) 25, 301, 343, 462, 484
Bme1390I CCNGG 4 cut(s) 107, 409, 425, 707
Bme18I GGWCC 1 cut(s) 502
BmgT120I GGNCC 3 cut(s) 484, 502, 507
BmiI GGNNCC 3 cut(s) 273, 405, 422
BmrFI CCNGG 4 cut(s) 107, 409, 425, 707
BmtI GCTAGC 1 cut(s) 795
BpmI CTGGAG 2 cut(s) 794, 804
BsaHI GRCGYC 2 cut(s) 26, 404
BsaI GGTCTC 1 cut(s) 135
BsaJI CCNNGG 5 cut(s) 75, 106, 255, 407, 510
BsaXI ACNNNNNCTCC 2 cut(s) 559, 589
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 430, 493, 511, 712
Bse118I RCCGGY 1 cut(s) 504
Bse1I ACTGG 1 cut(s) 118
Bse3DI GCAATG 1 cut(s) 766
BseBI CCWGG 4 cut(s) 107, 409, 425, 707
BseDI CCNNGG 5 cut(s) 75, 106, 255, 407, 510
BseGI GGATG 2 cut(s) 150, 417
BseLI CCNNNNNNNGG 5 cut(s) 76, 430, 493, 511, 712
BseMI GCAATG 1 cut(s) 766
BseMII CTCAG 1 cut(s) 668
BseNI ACTGG 1 cut(s) 118
BseRI GAGGAG 7 cut(s) 323, 326, 404, 488, 530, 538, 583
BseXI GCAGC 1 cut(s) 472
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 6 cut(s) 171, 254, 263, 485, 508, 692
BshNI GGYRCC 3 cut(s) 271, 403, 420
BsiHKAI GWGCWC 2 cut(s) 54, 446
BsiSI CCGG 1 cut(s) 505
BslFI GGGAC 1 cut(s) 13
BslI CCNNNNNNNGG 5 cut(s) 76, 430, 493, 511, 712
BsmAI GTCTC 3 cut(s) 135, 281, 655
BsmBI CGTCTC 1 cut(s) 281
BsmFI GGGAC 1 cut(s) 13
BsmI GAATGC 1 cut(s) 703
BsnI GGCC 6 cut(s) 171, 254, 263, 485, 508, 692
Bso31I GGTCTC 1 cut(s) 135
Bsp1286I GDGCHC 2 cut(s) 54, 446
Bsp143I GATC 6 cut(s) 39, 58, 187, 201, 702, 750
Bsp19I CCATGG 1 cut(s) 75
Bsp68I TCGCGA 1 cut(s) 248
BspANI GGCC 6 cut(s) 171, 254, 263, 485, 508, 692
BspCNI CTCAG 1 cut(s) 669
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 3 cut(s) 273, 405, 422
BspMAI CTGCAG 1 cut(s) 660
BspOI GCTAGC 1 cut(s) 795
BspPI GGATC 1 cut(s) 697
BspT107I GGYRCC 3 cut(s) 271, 403, 420
BspTNI GGTCTC 1 cut(s) 135
BsrBI CCGCTC 2 cut(s) 23, 573
BsrDI GCAATG 1 cut(s) 766
BsrFI RCCGGY 1 cut(s) 504
BsrI ACTGG 1 cut(s) 118
BssAI RCCGGY 1 cut(s) 504
BssECI CCNNGG 5 cut(s) 75, 106, 255, 407, 510
BssMI GATC 6 cut(s) 39, 58, 187, 201, 702, 750
BssNI GRCGYC 2 cut(s) 26, 404
BssT1I CCWWGG 2 cut(s) 75, 510
Bst2UI CCWGG 4 cut(s) 107, 409, 425, 707
Bst4CI ACNGT 1 cut(s) 271
Bst6I CTCTTC 2 cut(s) 324, 330
BstACI GRCGYC 2 cut(s) 26, 404
BstC8I GCNNGC 3 cut(s) 173, 780, 793
BstDEI CTNAG 1 cut(s) 677
BstDSI CCRYGG 2 cut(s) 75, 255
BstF5I GGATG 2 cut(s) 150, 417
BstFNI CGCG 1 cut(s) 248
BstH2I RGCGCY 2 cut(s) 347, 407
BstHHI GCGC 2 cut(s) 346, 406
BstKTI GATC 6 cut(s) 42, 61, 190, 204, 705, 753
BstMAI GTCTC 3 cut(s) 135, 281, 655
BstMBI GATC 6 cut(s) 39, 58, 187, 201, 702, 750
BstMWI GCNNNNNNNGC 7 cut(s) 32, 260, 299, 640, 698, 707, 788
BstNI CCWGG 4 cut(s) 107, 409, 425, 707
BstSCI CCNGG 4 cut(s) 105, 407, 423, 705
BstSFI CTRYAG 1 cut(s) 656
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 1 cut(s) 472
BsuI GTATCC 1 cut(s) 593
BsuRI GGCC 6 cut(s) 171, 254, 263, 485, 508, 692
BtgI CCRYGG 2 cut(s) 75, 255
BtsCI GGATG 2 cut(s) 150, 417
BtsIMutI CAGTG 1 cut(s) 656
BtuMI TCGCGA 1 cut(s) 248
Cac8I GCNNGC 3 cut(s) 173, 780, 793
CaiI CAGNNNCTG 1 cut(s) 655
CfoI GCGC 2 cut(s) 346, 406
Cfr10I RCCGGY 1 cut(s) 504
Cfr13I GGNCC 3 cut(s) 484, 502, 507
CseI GACGC 1 cut(s) 15
CsiI ACCWGGT 1 cut(s) 423
Csp6I GTAC 3 cut(s) 121, 421, 451
CviAII CATG 1 cut(s) 76
CviQI GTAC 3 cut(s) 121, 421, 451
DdeI CTNAG 1 cut(s) 677
DinI GGCGCC 1 cut(s) 405
DpnI GATC 6 cut(s) 41, 60, 189, 203, 704, 752
DpnII GATC 6 cut(s) 39, 58, 187, 201, 702, 750
EaeI YGGCCR 1 cut(s) 690
Eam1104I CTCTTC 2 cut(s) 324, 330
EarI CTCTTC 2 cut(s) 324, 330
EciI GGCGGA 2 cut(s) 308, 401
Ecl136II GAGCTC 1 cut(s) 52
Eco130I CCWWGG 2 cut(s) 75, 510
Eco24I GRGCYC 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 135
Eco47I GGWCC 1 cut(s) 502
Eco53kI GAGCTC 1 cut(s) 52
Eco57I CTGAAG 1 cut(s) 121
EcoICRI GAGCTC 1 cut(s) 52
EcoRII CCWGG 4 cut(s) 105, 407, 423, 705
EcoT14I CCWWGG 2 cut(s) 75, 510
EcoT38I GRGCYC 1 cut(s) 54
EgeI GGCGCC 1 cut(s) 405
EheI GGCGCC 1 cut(s) 405
ErhI CCWWGG 2 cut(s) 75, 510
Esp3I CGTCTC 1 cut(s) 281
FaeI CATG 1 cut(s) 79
FaiI YATR 3 cut(s) 77, 437, 618
FaqI GGGAC 1 cut(s) 13
FatI CATG 1 cut(s) 75
FbaI TGATCA 1 cut(s) 58
Fnu4HI GCNGC 5 cut(s) 24, 300, 342, 461, 483
FokI GGATG 2 cut(s) 137, 424
FriOI GRGCYC 1 cut(s) 54
Fsp4HI GCNGC 5 cut(s) 24, 300, 342, 461, 483
FspBI CTAG 2 cut(s) 242, 792
GlaI GCGC 2 cut(s) 345, 405
GluI GCNGC 5 cut(s) 24, 300, 342, 461, 483
GsuI CTGGAG 2 cut(s) 794, 804
HaeII RGCGCY 2 cut(s) 347, 407
HaeIII GGCC 6 cut(s) 171, 254, 263, 485, 508, 692
HapII CCGG 1 cut(s) 505
HgaI GACGC 1 cut(s) 15
HhaI GCGC 2 cut(s) 346, 406
Hin1I GRCGYC 2 cut(s) 26, 404
Hin1II CATG 1 cut(s) 79
Hin6I GCGC 2 cut(s) 344, 404
HinP1I GCGC 2 cut(s) 344, 404
HinfI GANTC 3 cut(s) 147, 206, 646
HpaII CCGG 1 cut(s) 505
HphI GGTGA 6 cut(s) 58, 338, 388, 532, 733, 754
Hpy166II GTNNAC 1 cut(s) 123
Hpy188I TCNGA 8 cut(s) 20, 39, 140, 360, 433, 533, 567, 678
Hpy188III TCNNGA 3 cut(s) 164, 191, 247
Hpy8I GTNNAC 1 cut(s) 123
Hpy99I CGWCG 3 cut(s) 215, 218, 221
HpyAV CCTTC 5 cut(s) 145, 161, 223, 244, 541
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4V TGCA 3 cut(s) 226, 460, 658
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 260, 299, 640, 698, 707, 788
HpyF3I CTNAG 1 cut(s) 677
Hsp92I GRCGYC 2 cut(s) 26, 404
Hsp92II CATG 1 cut(s) 79
HspAI GCGC 2 cut(s) 344, 404
KasI GGCGCC 1 cut(s) 403
KpnI GGTACC 1 cut(s) 424
Ksp22I TGATCA 1 cut(s) 58
Kzo9I GATC 6 cut(s) 39, 58, 187, 201, 702, 750
LmnI GCTCC 5 cut(s) 20, 40, 233, 570, 775
Lsp1109I GCAGC 1 cut(s) 472
MabI ACCWGGT 1 cut(s) 423
MaeI CTAG 2 cut(s) 242, 792
MaeIII GTNAC 1 cut(s) 64
MalI GATC 6 cut(s) 41, 60, 189, 203, 704, 752
MbiI CCGCTC 2 cut(s) 23, 573
MboI GATC 6 cut(s) 39, 58, 187, 201, 702, 750
MboII GAAGA 6 cut(s) 34, 94, 341, 347, 406, 622
MhlI GDGCHC 2 cut(s) 54, 446
MlsI TGGCCA 1 cut(s) 692
MluCI AATT 2 cut(s) 130, 221
MluNI TGGCCA 1 cut(s) 692
Mly113I GGCGCC 1 cut(s) 404
MlyI GAGTC 1 cut(s) 215
MmeI TCCRAC 1 cut(s) 480
Mox20I TGGCCA 1 cut(s) 692
MscI TGGCCA 1 cut(s) 692
Msp20I TGGCCA 1 cut(s) 692
MspI CCGG 1 cut(s) 505
MspR9I CCNGG 4 cut(s) 107, 409, 425, 707
Mva1269I GAATGC 1 cut(s) 703
MvaI CCWGG 4 cut(s) 107, 409, 425, 707
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 7 cut(s) 32, 260, 299, 640, 698, 707, 788
NarI GGCGCC 1 cut(s) 404
NcoI CCATGG 1 cut(s) 75
NdeII GATC 6 cut(s) 39, 58, 187, 201, 702, 750
NheI GCTAGC 1 cut(s) 791
NlaIII CATG 1 cut(s) 79
NlaIV GGNNCC 3 cut(s) 273, 405, 422
NmuCI GTSAC 1 cut(s) 64
NruI TCGCGA 1 cut(s) 248
PcsI WCGNNNNNNNCGW 5 cut(s) 192, 201, 210, 252, 461
PctI GAATGC 1 cut(s) 703
PfeI GAWTC 2 cut(s) 147, 646
PflMI CCANNNNNTGG 2 cut(s) 76, 712
PkrI GCNGC 5 cut(s) 25, 301, 343, 462, 484
PleI GAGTC 1 cut(s) 214
PluTI GGCGCC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 54
Psp6I CCWGG 4 cut(s) 105, 407, 423, 705
PspGI CCWGG 4 cut(s) 105, 407, 423, 705
PspN4I GGNNCC 3 cut(s) 273, 405, 422
PspPI GGNCC 3 cut(s) 484, 502, 507
PstI CTGCAG 1 cut(s) 660
PstNI CAGNNNCTG 1 cut(s) 655
RruI TCGCGA 1 cut(s) 248
RsaI GTAC 3 cut(s) 122, 422, 452
RsaNI GTAC 3 cut(s) 121, 421, 451
SacI GAGCTC 1 cut(s) 54
SatI GCNGC 5 cut(s) 24, 300, 342, 461, 483
Sau3AI GATC 6 cut(s) 39, 58, 187, 201, 702, 750
Sau96I GGNCC 3 cut(s) 484, 502, 507
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 4 cut(s) 107, 409, 425, 707
SduI GDGCHC 2 cut(s) 54, 446
SexAI ACCWGGT 1 cut(s) 423
SfcI CTRYAG 1 cut(s) 656
SfiI GGCCNNNNNGGCC 1 cut(s) 260
SfoI GGCGCC 1 cut(s) 405
SinI GGWCC 1 cut(s) 502
Sse9I AATT 2 cut(s) 130, 221
SspDI GGCGCC 1 cut(s) 403
SspMI CTAG 2 cut(s) 242, 792
SstI GAGCTC 1 cut(s) 54
StyD4I CCNGG 4 cut(s) 105, 407, 423, 705
StyI CCWWGG 2 cut(s) 75, 510
TaaI ACNGT 1 cut(s) 271
TaqI TCGA 3 cut(s) 204, 219, 753
TaqII GACCGA 1 cut(s) 159
TasI AATT 2 cut(s) 130, 221
TatI WGTACW 2 cut(s) 120, 450
TauI GCSGC 4 cut(s) 26, 302, 344, 485
TfiI GAWTC 2 cut(s) 147, 646
TscAI CASTG 1 cut(s) 656
TseFI GTSAC 1 cut(s) 64
TseI GCWGC 1 cut(s) 460
Tsp45I GTSAC 1 cut(s) 64
TspGWI ACGGA 1 cut(s) 199
TspRI CASTG 1 cut(s) 656
Van91I CCANNNNNTGG 2 cut(s) 76, 712
VpaK11BI GGWCC 1 cut(s) 502
XspI CTAG 2 cut(s) 242, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.