Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0016561

Physical Location & Seq

Genomic Coordinates Reverse (-)

15954885 .. 15956238

Gene Structure 1354 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000012171

Sequence Viewer

CDS Protein Promoter (2k)

Length: 966 bp

ATGGCGAGCAGTGGCAGTGGGCAACAACTGCCGCCGTACAACCCTTACCGGCACCTGAAGACCCTTAGCGGCCACGAGAGCGCCGTGTCGTGCGTCAAATTCTCCAACGACGGAACCCTCTTGGCCTCGGCGTCGTTAGACAAGACCGTGATCCTCTGGTTGTCTTCCACCCTAGCCCTCGAGCGCCGCCTGGTGGGACACTCCGAGGGCATCTCCGACCTTTCCTGGTCCTCCGACTCCCACTACATCTGCTCCGCCTCCGACGACCGCACCCTCCGCATCTGGGACGCACGCTCCGGCGAGTGCGTCAAGACCCTCCGCGGCCACTCCGACCTCGTTTTCTGCGCCAACTTCAACCCCCAGTCCAACCTCATCGTCTCCGGCTCGTTCGACGAGACGATTCGGATTTGGGAGGTCAAGACCGGGAAGTGCCTCCACGTCATCAGAGCCCACTCGCTTCCGGTGACCTCCGTGAGCTTCAATCGCGATGGGTCGCTCATCGTCTCCGCCAGTCACGACGGGTCCTGCAAGATTTGGGACACCGCTAAAGGGACCTGCTTGAAAACCCTAATTGACGATAAGGTCCCTGCCGTTTCCTTCGCCAAGTTCTCCCCCAACGGAAAATTCGTACTCATCGCCACTCTAGATAATACCCTTAAGCTGTGCAACTACTCGACGGGAAAGTTCCTGAAGGTCTATACGGGGCATTTGAACAAAGTGTATTGCATAACATCTACATTTTCTGTGACAAATGGGAAATATATTGTTAGTGGTTCCGAGGATCACTGTGTATACTTGTGGGATCTTCAGTCGAAAACTATGATTCAGAGATTTGAAGGCCATACTGATACCGTCATCTCTGTTTCCTGTCACCCGTCGGAGAATAAGATTGCCTCCGCAGCTCTCCAAAATGATAAGACTGTCCGGATTTGGGTTCAAGATAATGCCAATTCAGCTCCTAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000123 GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005815 GO:0005856 GO:0005929 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006479 GO:0006807 GO:0006996 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0008213 GO:0009987 GO:0015630 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016573 GO:0018022 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019538 GO:0022008 GO:0022607 GO:0030030 GO:0030031 GO:0030154 GO:0030182 GO:0031175 GO:0031248 GO:0031461 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032991 GO:0034708 GO:0034968 GO:0035097 GO:0036064 GO:0036211 GO:0042995 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043543 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044441 GO:0044446 GO:0044451 GO:0044463 GO:0044464 GO:0044665 GO:0044782 GO:0048188 GO:0048468 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0051276 GO:0051568 GO:0060271 GO:0070013 GO:0070925 GO:0071339 GO:0071704 GO:0071840 GO:0080008 GO:0120025 GO:0120031 GO:0120036 GO:0120038 GO:1901564 GO:1902493 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04934 map04934

Pfam Domains

Protein Families

WD40

Protein Analysis

322

Amino Acids

35.2

Weight (kDa)

8.43

Isoelectric Point (pI)

30.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_MABP1-WDR62_2nd	PF24782	15 - 179	1.2e-17	MABP1/WDR62 second WD40 domain
WDR55	PF24796	17 - 143	7e-17	WDR55
Beta-prop_TEP1_2nd	PF25047	17 - 111	8.2e-13	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	17 - 138	2.4e-23	WDR3 second beta-propeller domain
WD40	PF00400	18 - 53	3.4e-11	WD domain, G-beta repeat
Beta-prop_EML_2	PF23414	18 - 106	6.9e-14	Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19	PF24814	18 - 105	2.7e-13	Prp19 WD40 domain
Beta-prop_IFT122_1st	PF23381	19 - 87	3.7e-06	IFT122 first beta-propeller
WD40_Gbeta	PF25391	20 - 184	2.2e-22	G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1	PF24105	20 - 156	8.3e-20	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	20 - 113	9.9e-23	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	21 - 311	5.3e-135	WDR5 beta-propeller domain
EIF3I	PF24805	21 - 106	1.9e-12	EIF3I
WD40_WDHD1_1st	PF24817	24 - 81	4.3e-06	WDHD1 first WD40 domain
Beta-prop_EML	PF23409	24 - 127	4.3e-09	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin	PF25460	24 - 102	6.4e-07	Aladin seven-bladed propeller
Beta-prop_THOC3	PF25174	25 - 111	3.9e-22	THOC3 beta-propeller domain
Beta-prop_WDR19_1st	PF23389	26 - 148	3.2e-07	WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	27 - 104	9.9e-10	WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz	PF24807	59 - 152	3e-15	CDC20/Fizzy WD40 domain
WD40	PF00400	62 - 96	3.8e-10	WD domain, G-beta repeat
EIF3I	PF24805	64 - 163	1e-16	EIF3I
WD40_WDHD1_1st	PF24817	69 - 148	3e-14	WDHD1 first WD40 domain
WD40_MABP1-WDR62_1st	PF24780	69 - 168	6.9e-09	MABP1/WDR62 first WD40 domain
WD40_Prp19	PF24814	69 - 274	3e-38	Prp19 WD40 domain
WDR90_beta-prop_4th	PF23342	72 - 197	3.2e-10	WDR90, 4th beta-propeller
NBCH_WD40	PF20426	74 - 143	4.8e-07	Neurobeachin beta propeller domain
Beta-prop_ACSF4	PF13570	76 - 187	1.3e-06	Beta-alanine-activating enzyme, beta-propeller
Beta-prop_WDR75_1st	PF23869	77 - 140	7e-09	WD repeat-containing protein 75 first beta-propeller
Beta-prop_EIPR1	PF23609	79 - 137	4.9e-06	EIPR1 beta-propeller
Beta-prop_WDR41	PF25178	79 - 150	1e-06	WDR41
Beta-prop_WDR90_POC16_2nd	PF23393	83 - 185	1.8e-11	WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	84 - 221	2.2e-14	WDR36/Utp21 first beta-propeller
Beta-prop_SCAP	PF24017	84 - 188	4.3e-09	SCAP Beta-propeller
WD40	PF00400	100 - 137	1.7e-10	WD domain, G-beta repeat
Beta-prop_WDR3_1st	PF25173	100 - 196	9.1e-23	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	101 - 163	2.6e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	101 - 195	2.2e-21	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	102 - 195	3.2e-11	TEP-1 second beta-propeller
Beta-prop_Aladin	PF25460	102 - 217	1.5e-10	Aladin seven-bladed propeller
WD40_Gbeta	PF25391	106 - 311	5.6e-20	G protein beta WD-40 repeat protein
WD40_WDHD1_1st	PF24817	113 - 278	3.2e-25	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	118 - 277	4.6e-19	Echinoderm microtubule-associated protein second beta-propeller
WDR55	PF24796	122 - 181	2.6e-08	WDR55
Beta-prop_EML	PF23409	132 - 213	7.3e-07	Echinoderm microtubule-associated protein first beta-propeller
WD40	PF00400	142 - 180	7.8e-12	WD domain, G-beta repeat
NBCH_WD40	PF20426	144 - 299	2.8e-09	Neurobeachin beta propeller domain
Beta-prop_WDR3_2nd	PF25172	145 - 200	8.2e-07	WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	146 - 229	5.6e-06	CAF1B/HIR1 beta-propeller domain
EIF3I	PF24805	148 - 232	4.9e-08	EIF3I
Beta-prop_WDR36-Utp21_2nd	PF25168	149 - 313	1.3e-13	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	167 - 314	2.8e-10	WDR36/Utp21 first beta-propeller
Beta-prop_TEP1_2nd	PF25047	196 - 312	4.5e-09	TEP-1 second beta-propeller
Beta-prop_WDR3_1st	PF25173	196 - 312	3.8e-17	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	197 - 316	1.1e-14	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd	PF25172	198 - 315	2.9e-12	WDR3 second beta-propeller domain
WDR55	PF24796	201 - 300	1.2e-09	WDR55
WD40_MABP1-WDR62_2nd	PF24782	203 - 269	3e-06	MABP1/WDR62 second WD40 domain
Beta-prop_IFT122_1st	PF23381	203 - 301	1.2e-06	IFT122 first beta-propeller
WD40_WDHD1_1st	PF24817	206 - 317	1.4e-12	WDHD1 first WD40 domain
Beta-prop_EML	PF23409	226 - 297	1.4e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40	PF00400	227 - 268	2.2e-07	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	229 - 314	7.7e-08	CAF1B/HIR1 beta-propeller domain
WD40_MABP1-WDR62_1st	PF24780	246 - 312	4.4e-07	MABP1/WDR62 first WD40 domain
WD40_Prp19	PF24814	249 - 317	5e-11	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	251 - 314	1.6e-12	THOC3 beta-propeller domain
WD40	PF00400	274 - 311	3.3e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

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Orthologous Genes (Group: OG0016561)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02730
fragaria_vesca	FvH4_2g23370
malus_domestica	MD03G1263200.v1.1
prunus_persica	Prupe.8G239500_v2.0.a1
pyrus_communis	pycom03g21070
rosa_chinensis	RchiOBHm_Chr6g0290821
rosa_laevigata	RLG00000012171
rosa_multiflora	Rmu_ssc0000069.1_g000002
rosa_roxburghii	Rroxscaffold_7G00176710
rosa_rugosa	Rorug06G0214900
rosa_samantha	Rh6BG333200 Rh6CG338900 Rh6DG326300
rosa_wichuraiana	Rw6G028300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	581
Acc36I	ACCTGC	1 cut(s)	563
AccB1I	GGYRCC	1 cut(s)	51
AccI	GTMKAC	1 cut(s)	792
AccII	CGCG	2 cut(s)	321, 486
AccIII	TCCGGA	1 cut(s)	924
AclWI	GGATC	3 cut(s)	145, 789, 810
AcoI	YGGCCR	2 cut(s)	70, 322
AcsI	RAATTY	2 cut(s)	98, 623
AcuI	CTGAAG	3 cut(s)	77, 710, 791
AcyI	GRCGYC	1 cut(s)	131
AfaI	GTAC	2 cut(s)	38, 630
AfiI	CCNNNNNNNGG	4 cut(s)	193, 283, 549, 931
AflII	CTTAAG	1 cut(s)	656
AgsI	TTSAA	6 cut(s)	355, 481, 562, 712, 836, 938
AjiI	CACGTC	1 cut(s)	439
AjnI	CCWGG	2 cut(s)	189, 224
AjuI	GAANNNNNNNTTGG	2 cut(s)	608, 640
AluBI	AGCT	4 cut(s)	477, 661, 902, 956
AluI	AGCT	4 cut(s)	477, 661, 902, 956
Alw26I	GTCTC	3 cut(s)	382, 389, 508
AlwI	GGATC	3 cut(s)	145, 789, 810
Ama87I	CYCGRG	1 cut(s)	179
Aor13HI	TCCGGA	1 cut(s)	924
AoxI	GGCC	4 cut(s)	70, 123, 322, 838
ApeKI	GCWGC	1 cut(s)	899
ApoI	RAATTY	2 cut(s)	98, 623
AspLEI	GCGC	3 cut(s)	83, 186, 347
AspS9I	GGNCC	4 cut(s)	228, 522, 552, 583
AsuC2I	CCSGG	1 cut(s)	424
AsuHPI	GGTGA	2 cut(s)	475, 863
AvaI	CYCGRG	1 cut(s)	179
AvaII	GGWCC	4 cut(s)	228, 522, 552, 583
BanI	GGYRCC	1 cut(s)	51
BanII	GRGCYC	1 cut(s)	451
BauI	CACGAG	1 cut(s)	74
BbsI	GAAGAC	2 cut(s)	65, 156
BbvI	GCAGC	1 cut(s)	911
BccI	CCATC	1 cut(s)	482
BceAI	ACGGC	3 cut(s)	19, 68, 575
BciT130I	CCWGG	2 cut(s)	191, 226
BcnI	CCSGG	1 cut(s)	424
BcoDI	GTCTC	3 cut(s)	382, 389, 508
BfaI	CTAG	2 cut(s)	173, 644
BfoI	RGCGCY	2 cut(s)	84, 187
BfrI	CTTAAG	1 cut(s)	656
BfuAI	ACCTGC	1 cut(s)	563
BisI	GCNGC	5 cut(s)	32, 70, 187, 322, 900
BlsI	GCNGC	5 cut(s)	33, 71, 188, 323, 901
Bme1390I	CCNGG	3 cut(s)	191, 226, 424
Bme18I	GGWCC	4 cut(s)	228, 522, 552, 583
BmeT110I	CYCGRG	1 cut(s)	179
BmgBI	CACGTC	1 cut(s)	439
BmgT120I	GGNCC	4 cut(s)	228, 522, 552, 583
BmiI	GGNNCC	6 cut(s)	53, 115, 523, 553, 585, 775
BmrFI	CCNGG	3 cut(s)	191, 226, 424
BmrI	ACTGGG	1 cut(s)	355
BmsI	GCATC	2 cut(s)	219, 288
BmuI	ACTGGG	1 cut(s)	355
BpiI	GAAGAC	2 cut(s)	65, 156
BplI	GAGNNNNNCTC	2 cut(s)	197, 229
Bpu10I	CCTNAGC	1 cut(s)	65
BpuMI	CCSGG	1 cut(s)	424
BsaHI	GRCGYC	1 cut(s)	131
BsaJI	CCNNGG	4 cut(s)	126, 204, 319, 777
BsaWI	WCCGGW	2 cut(s)	460, 924
BsaXI	ACNNNNNCTCC	4 cut(s)	236, 266, 278, 308
Bsc4I	CCNNNNNNNGG	4 cut(s)	193, 283, 549, 931
Bse118I	RCCGGY	1 cut(s)	48
Bse1I	ACTGG	2 cut(s)	361, 510
BseAI	TCCGGA	1 cut(s)	924
BseBI	CCWGG	2 cut(s)	191, 226
BseDI	CCNNGG	4 cut(s)	126, 204, 319, 777
BseLI	CCNNNNNNNGG	4 cut(s)	193, 283, 549, 931
BseNI	ACTGG	2 cut(s)	361, 510
BseXI	GCAGC	1 cut(s)	911
Bsh1236I	CGCG	2 cut(s)	321, 486
Bsh1285I	CGRYCG	1 cut(s)	268
BshFI	GGCC	4 cut(s)	72, 125, 324, 840
BshNI	GGYRCC	1 cut(s)	51
BsiEI	CGRYCG	1 cut(s)	268
BsiHKCI	CYCGRG	1 cut(s)	179
BsiSI	CCGG	6 cut(s)	49, 297, 381, 423, 461, 925
BslFI	GGGAC	5 cut(s)	210, 299, 551, 565, 569
BslI	CCNNNNNNNGG	4 cut(s)	193, 283, 549, 931
BsmAI	GTCTC	3 cut(s)	382, 389, 508
BsmBI	CGTCTC	3 cut(s)	382, 389, 508
BsmFI	GGGAC	5 cut(s)	210, 299, 551, 565, 569
BsnI	GGCC	4 cut(s)	72, 125, 324, 840
BsoBI	CYCGRG	1 cut(s)	179
Bsp1286I	GDGCHC	1 cut(s)	451
Bsp13I	TCCGGA	1 cut(s)	924
Bsp143I	GATC	3 cut(s)	150, 781, 802
Bsp68I	TCGCGA	1 cut(s)	486
BspANI	GGCC	4 cut(s)	72, 125, 324, 840
BspEI	TCCGGA	1 cut(s)	924
BspFNI	CGCG	2 cut(s)	321, 486
BspLI	GGNNCC	6 cut(s)	53, 115, 523, 553, 585, 775
BspMI	ACCTGC	1 cut(s)	563
BspPI	GGATC	3 cut(s)	145, 789, 810
BspT107I	GGYRCC	1 cut(s)	51
BspTI	CTTAAG	1 cut(s)	656
BsrFI	RCCGGY	1 cut(s)	48
BsrI	ACTGG	2 cut(s)	361, 510
BssAI	RCCGGY	1 cut(s)	48
BssECI	CCNNGG	4 cut(s)	126, 204, 319, 777
BssMI	GATC	3 cut(s)	150, 781, 802
BssNAI	GTATAC	1 cut(s)	793
BssNI	GRCGYC	1 cut(s)	131
BssSI	CACGAG	1 cut(s)	74
Bst1107I	GTATAC	1 cut(s)	793
Bst2BI	CACGAG	1 cut(s)	74
Bst2UI	CCWGG	2 cut(s)	191, 226
Bst4CI	ACNGT	4 cut(s)	148, 788, 853, 922
BstACI	GRCGYC	1 cut(s)	131
BstAFI	CTTAAG	1 cut(s)	656
BstAPI	GCANNNNNTGC	1 cut(s)	28
BstC8I	GCNNGC	2 cut(s)	7, 292
BstDEI	CTNAG	1 cut(s)	65
BstDSI	CCRYGG	1 cut(s)	319
BstEII	GGTNACC	1 cut(s)	463
BstFNI	CGCG	2 cut(s)	321, 486
BstH2I	RGCGCY	2 cut(s)	84, 187
BstHHI	GCGC	3 cut(s)	83, 186, 347
BstKTI	GATC	3 cut(s)	153, 784, 805
BstMAI	GTCTC	3 cut(s)	382, 389, 508
BstMBI	GATC	3 cut(s)	150, 781, 802
BstMCI	CGRYCG	1 cut(s)	268
BstMWI	GCNNNNNNNGC	6 cut(s)	28, 78, 276, 483, 899, 953
BstNI	CCWGG	2 cut(s)	191, 226
BstPI	GGTNACC	1 cut(s)	463
BstSCI	CCNGG	3 cut(s)	189, 224, 422
BstUI	CGCG	2 cut(s)	321, 486
BstV1I	GCAGC	1 cut(s)	911
BstV2I	GAAGAC	2 cut(s)	65, 156
BstX2I	RGATCY	1 cut(s)	802
BstYI	RGATCY	1 cut(s)	802
BstZ17I	GTATAC	1 cut(s)	793
BsuRI	GGCC	4 cut(s)	72, 125, 324, 840
BtgI	CCRYGG	1 cut(s)	319
BtgZI	GCGATG	2 cut(s)	501, 619
BtrI	CACGTC	1 cut(s)	439
BtsI	GCAGTG	2 cut(s)	16, 22
BtsIMutI	CAGTG	3 cut(s)	16, 22, 784
BtuMI	TCGCGA	1 cut(s)	486
BveI	ACCTGC	1 cut(s)	563
Cac8I	GCNNGC	2 cut(s)	7, 292
CfoI	GCGC	3 cut(s)	83, 186, 347
Cfr10I	RCCGGY	1 cut(s)	48
Cfr13I	GGNCC	4 cut(s)	228, 522, 552, 583
Cfr42I	CCGCGG	1 cut(s)	322
CseI	GACGC	4 cut(s)	82, 120, 295, 296
Csp6I	GTAC	2 cut(s)	37, 629
CviQI	GTAC	2 cut(s)	37, 629
DdeI	CTNAG	1 cut(s)	65
DpnI	GATC	3 cut(s)	152, 783, 804
DpnII	GATC	3 cut(s)	150, 781, 802
DrdI	GACNNNNNNGTC	1 cut(s)	581
DseDI	GACNNNNNNGTC	1 cut(s)	581
EaeI	YGGCCR	2 cut(s)	70, 322
EciI	GGCGGA	2 cut(s)	244, 496
Eco24I	GRGCYC	1 cut(s)	451
Eco47I	GGWCC	4 cut(s)	228, 522, 552, 583
Eco57I	CTGAAG	3 cut(s)	77, 710, 791
Eco88I	CYCGRG	1 cut(s)	179
Eco91I	GGTNACC	1 cut(s)	463
EcoO109I	RGGNCCY	3 cut(s)	522, 552, 583
EcoO65I	GGTNACC	1 cut(s)	463
EcoRII	CCWGG	2 cut(s)	189, 224
EcoT38I	GRGCYC	1 cut(s)	451
Esp3I	CGTCTC	3 cut(s)	382, 389, 508
FaiI	YATR	6 cut(s)	699, 728, 762, 793, 821, 843
FaqI	GGGAC	5 cut(s)	210, 299, 551, 565, 569
FblI	GTMKAC	1 cut(s)	792
Fnu4HI	GCNGC	5 cut(s)	32, 70, 187, 322, 900
FriOI	GRGCYC	1 cut(s)	451
Fsp4HI	GCNGC	5 cut(s)	32, 70, 187, 322, 900
FspBI	CTAG	2 cut(s)	173, 644
GlaI	GCGC	3 cut(s)	82, 185, 346
GluI	GCNGC	5 cut(s)	32, 70, 187, 322, 900
HaeII	RGCGCY	2 cut(s)	84, 187
HaeIII	GGCC	4 cut(s)	72, 125, 324, 840
HapII	CCGG	6 cut(s)	49, 297, 381, 423, 461, 925
HgaI	GACGC	4 cut(s)	82, 120, 295, 296
HhaI	GCGC	3 cut(s)	83, 186, 347
Hin1I	GRCGYC	1 cut(s)	131
Hin6I	GCGC	3 cut(s)	81, 184, 345
HinP1I	GCGC	3 cut(s)	81, 184, 345
HinfI	GANTC	3 cut(s)	236, 400, 823
HpaII	CCGG	6 cut(s)	49, 297, 381, 423, 461, 925
HphI	GGTGA	2 cut(s)	475, 863
Hpy166II	GTNNAC	1 cut(s)	793
Hpy188III	TCNNGA	8 cut(s)	310, 418, 485, 515, 644, 688, 925, 938
Hpy8I	GTNNAC	1 cut(s)	793
Hpy99I	CGWCG	7 cut(s)	113, 136, 266, 395, 521, 679, 880
HpyAV	CCTTC	3 cut(s)	607, 685, 830
HpyCH4III	ACNGT	4 cut(s)	148, 788, 853, 922
HpyCH4IV	ACGT	1 cut(s)	438
HpyCH4V	TGCA	3 cut(s)	528, 666, 726
HpyF10VI	GCNNNNNNNGC	6 cut(s)	28, 78, 276, 483, 899, 953
HpyF3I	CTNAG	1 cut(s)	65
HpySE526I	ACGT	1 cut(s)	438
Hsp92I	GRCGYC	1 cut(s)	131
HspAI	GCGC	3 cut(s)	81, 184, 345
Kpn2I	TCCGGA	1 cut(s)	924
KspI	CCGCGG	1 cut(s)	322
Kzo9I	GATC	3 cut(s)	150, 781, 802
LmnI	GCTCC	3 cut(s)	257, 299, 961
Lsp1109I	GCAGC	1 cut(s)	911
LweI	GCATC	2 cut(s)	219, 288
MaeI	CTAG	2 cut(s)	173, 644
MaeII	ACGT	1 cut(s)	438
MaeIII	GTNAC	4 cut(s)	463, 512, 745, 869
MalI	GATC	3 cut(s)	152, 783, 804
MboI	GATC	3 cut(s)	150, 781, 802
MboII	GAAGA	3 cut(s)	70, 156, 797
MflI	RGATCY	1 cut(s)	802
MhlI	GDGCHC	1 cut(s)	451
MluCI	AATT	4 cut(s)	98, 570, 623, 949
MlyI	GAGTC	1 cut(s)	230
MmeI	TCCRAC	7 cut(s)	129, 240, 258, 285, 354, 390, 858
MroI	TCCGGA	1 cut(s)	924
MseI	TTAA	1 cut(s)	657
MspA1I	CMGCKG	1 cut(s)	321
MspCI	CTTAAG	1 cut(s)	656
MspI	CCGG	6 cut(s)	49, 297, 381, 423, 461, 925
MspR9I	CCNGG	3 cut(s)	191, 226, 424
MvaI	CCWGG	2 cut(s)	191, 226
MvnI	CGCG	2 cut(s)	321, 486
MwoI	GCNNNNNNNGC	6 cut(s)	28, 78, 276, 483, 899, 953
NciI	CCSGG	1 cut(s)	424
NdeII	GATC	3 cut(s)	150, 781, 802
NlaIV	GGNNCC	6 cut(s)	53, 115, 523, 553, 585, 775
NmeAIII	GCCGAG	1 cut(s)	107
NmuCI	GTSAC	4 cut(s)	463, 512, 745, 869
NruI	TCGCGA	1 cut(s)	486
PaeR7I	CTCGAG	1 cut(s)	179
PcsI	WCGNNNNNNNCGW	2 cut(s)	81, 624
PfeI	GAWTC	2 cut(s)	400, 823
PkrI	GCNGC	5 cut(s)	33, 71, 188, 323, 901
PleI	GAGTC	1 cut(s)	230
PpsI	GAGTC	1 cut(s)	230
PpuMI	RGGWCCY	3 cut(s)	522, 552, 583
Psp5II	RGGWCCY	3 cut(s)	522, 552, 583
Psp6I	CCWGG	2 cut(s)	189, 224
PspEI	GGTNACC	1 cut(s)	463
PspGI	CCWGG	2 cut(s)	189, 224
PspN4I	GGNNCC	6 cut(s)	53, 115, 523, 553, 585, 775
PspPI	GGNCC	4 cut(s)	228, 522, 552, 583
PspPPI	RGGWCCY	3 cut(s)	522, 552, 583
PspXI	VCTCGAGB	1 cut(s)	179
PsuI	RGATCY	1 cut(s)	802
RruI	TCGCGA	1 cut(s)	486
RsaI	GTAC	2 cut(s)	38, 630
RsaNI	GTAC	2 cut(s)	37, 629
SacII	CCGCGG	1 cut(s)	322
SaqAI	TTAA	1 cut(s)	657
SatI	GCNGC	5 cut(s)	32, 70, 187, 322, 900
Sau3AI	GATC	3 cut(s)	150, 781, 802
Sau96I	GGNCC	4 cut(s)	228, 522, 552, 583
SchI	GAGTC	1 cut(s)	230
ScrFI	CCNGG	3 cut(s)	191, 226, 424
SduI	GDGCHC	1 cut(s)	451
SfaNI	GCATC	2 cut(s)	219, 288
Sfr274I	CTCGAG	1 cut(s)	179
Sfr303I	CCGCGG	1 cut(s)	322
SgrBI	CCGCGG	1 cut(s)	322
SinI	GGWCC	4 cut(s)	228, 522, 552, 583
SlaI	CTCGAG	1 cut(s)	179
SmlI	CTYRAG	2 cut(s)	179, 656
SmoI	CTYRAG	2 cut(s)	179, 656
Sse9I	AATT	4 cut(s)	98, 570, 623, 949
SspMI	CTAG	2 cut(s)	173, 644
StyD4I	CCNGG	3 cut(s)	189, 224, 422
TaaI	ACNGT	4 cut(s)	148, 788, 853, 922
TaiI	ACGT	1 cut(s)	441
TaqI	TCGA	4 cut(s)	180, 390, 674, 812
TasI	AATT	4 cut(s)	98, 570, 623, 949
TauI	GCSGC	4 cut(s)	34, 72, 189, 324
TfiI	GAWTC	2 cut(s)	400, 823
Tru1I	TTAA	1 cut(s)	657
Tru9I	TTAA	1 cut(s)	657
TscAI	CASTG	3 cut(s)	16, 22, 791
TseFI	GTSAC	4 cut(s)	463, 512, 745, 869
TseI	GCWGC	1 cut(s)	899
Tsp45I	GTSAC	4 cut(s)	463, 512, 745, 869
TspGWI	ACGGA	3 cut(s)	126, 460, 633
TspRI	CASTG	3 cut(s)	16, 22, 791
Vha464I	CTTAAG	1 cut(s)	656
VpaK11BI	GGWCC	4 cut(s)	228, 522, 552, 583
XapI	RAATTY	2 cut(s)	98, 623
XbaI	TCTAGA	1 cut(s)	643
XhoI	CTCGAG	1 cut(s)	179
XmiI	GTMKAC	1 cut(s)	792
XspI	CTAG	2 cut(s)	173, 644

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.